AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1163270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1163270 MtrunA17_Chr7g0260011 96.565 262 9 0 10 271 1 262 0.0 523
Msa1163270 MtrunA17_Chr1g0185331 56.977 258 88 9 10 266 1 236 4.65e-83 249
Msa1163270 MtrunA17_Chr1g0205211 36.897 290 110 12 40 265 6 286 3.06e-38 135
Msa1163270 MtrunA17_Chr3g0138651 41.259 143 55 4 155 270 258 398 1.47e-21 93.2
Msa1163270 MtrunA17_Chr1g0148411 42.373 118 46 3 173 270 204 319 1.78e-19 86.7
Msa1163270 MtrunA17_Chr2g0331941 43.011 93 41 3 173 256 261 350 3.47e-17 80.5
Msa1163270 MtrunA17_Chr8g0344991 56.522 46 20 0 224 269 39 84 1.23e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1163270 AT3G63210.1 38.710 279 133 12 11 267 1 263 1.37e-42 146
Msa1163270 AT3G22550.1 36.879 282 126 11 11 262 1 260 5.04e-42 145
Msa1163270 AT5G11460.1 26.394 269 117 6 68 260 44 307 3.81e-22 94.4
Msa1163270 AT2G25690.2 45.833 96 43 4 182 270 218 311 6.76e-17 79.3
Msa1163270 AT2G25690.1 45.833 96 43 4 182 270 218 311 6.76e-17 79.3
Msa1163270 AT1G53885.1 45.588 68 37 0 202 269 20 87 2.53e-13 65.9
Msa1163270 AT1G53903.1 45.588 68 37 0 202 269 20 87 2.53e-13 65.9

Find 65 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTAGTATCTAGCTATTT+TGG 0.059941 7_4:-54413702 Msa1163270:five_prime_UTR
AAAACTATCCCTTCTTTATT+TGG 0.094272 7_4:-54413471 Msa1163270:CDS
CCAAGTAATGACTCTTAATT+TGG 0.147550 7_4:-54412218 Msa1163270:three_prime_UTR
TTGAAATCTCTAATAAATTT+TGG 0.167799 7_4:+54413444 None:intergenic
CAAAAGATATATTTATTTAT+AGG 0.182500 7_4:-54412882 Msa1163270:intron
GATAAATCAAAGTGGTTTAT+TGG 0.185687 7_4:-54413741 Msa1163270:five_prime_UTR
CCAAAAGGCTTGACTATTAT+AGG 0.223407 7_4:-54413778 Msa1163270:five_prime_UTR
CTGTGATCAGCCATTAAATT+TGG 0.239971 7_4:+54413531 None:intergenic
ACTCTTAATTTGGTTGCTTC+TGG 0.254056 7_4:-54412208 Msa1163270:three_prime_UTR
CCTATAATAGTCAAGCCTTT+TGG 0.291226 7_4:+54413778 None:intergenic
GGCATTGGTCTTGCTCTTAT+AGG 0.291963 7_4:-54413270 Msa1163270:CDS
AACAATTCTAACTCATCTTA+TGG 0.293044 7_4:-54412952 Msa1163270:CDS
ATGTTATCCCCTTATGGTTT+TGG 0.298858 7_4:-54413354 Msa1163270:CDS
AGTGACCAAGCCAAATTTAA+TGG 0.303564 7_4:-54413541 Msa1163270:CDS
ACTAGAATGTTATCCCCTTA+TGG 0.303568 7_4:-54413360 Msa1163270:CDS
TCGAATATATGAGTGGTTCT+TGG 0.324933 7_4:+54413015 None:intergenic
ATATGAGTGGTTCTTGGATT+AGG 0.342533 7_4:+54413021 None:intergenic
CCAAATTAAGAGTCATTACT+TGG 0.351609 7_4:+54412218 None:intergenic
CTCACTCAAAGAAAGAAGTT+TGG 0.359826 7_4:+54413086 None:intergenic
CAATTGAATCATCATCCTTA+AGG 0.370108 7_4:+54413244 None:intergenic
TTCTGAGAGAGAACTATTGT+TGG 0.407584 7_4:+54413413 None:intergenic
CTGCTTCATCCTAAAACAAT+GGG 0.411662 7_4:+54412177 None:intergenic
TAGAGTGCATATTTGAAATC+AGG 0.419096 7_4:-54412484 Msa1163270:three_prime_UTR
AATATAGGAGATGGCAAAGT+TGG 0.431921 7_4:+54413153 None:intergenic
TGCTCTTATAGGTAACCTTA+AGG 0.442219 7_4:-54413259 Msa1163270:CDS
ACTTCTTTCTTTGAGTGAGA+TGG 0.446621 7_4:-54413082 Msa1163270:CDS
TTTGGTGAACCAAATAAAGA+AGG 0.452111 7_4:+54413462 None:intergenic
GTGGATAACCAAAACCATAA+GGG 0.454650 7_4:+54413346 None:intergenic
CACAGAGAAATGGTGTTAGA+TGG 0.461833 7_4:-54412629 Msa1163270:CDS
TAGTGTCAAGAATTGAAGTT+GGG 0.468986 7_4:+54413379 None:intergenic
AGTGGATAACCAAAACCATA+AGG 0.471666 7_4:+54413345 None:intergenic
ACCTTCTCATCAAGAGGCAT+TGG 0.472949 7_4:-54413285 Msa1163270:CDS
ATCAGCCATTAAATTTGGCT+TGG 0.474809 7_4:+54413536 None:intergenic
TTTGTCTTTGAGCTCTCCAA+TGG 0.480930 7_4:-54412275 Msa1163270:three_prime_UTR
TCTCTTATCTCTATATCCAT+TGG 0.489719 7_4:+54412259 None:intergenic
CAAATTCCGTAAGTGTGGTT+CGG 0.500194 7_4:-54412418 Msa1163270:three_prime_UTR
GACTCAAATGGAGAAAATAT+AGG 0.506068 7_4:+54413138 None:intergenic
TATACTTGTGTGATCTCTCA+TGG 0.508508 7_4:-54413045 Msa1163270:CDS
AGAATGCAGGCACAGAGAAA+TGG 0.509379 7_4:-54412639 Msa1163270:CDS
ACCAATGCCTCTTGATGAGA+AGG 0.509626 7_4:+54413284 None:intergenic
AGTACTAATGCTTATTCTTG+AGG 0.509882 7_4:-54412580 Msa1163270:three_prime_UTR
CTAGTGTCAAGAATTGAAGT+TGG 0.516669 7_4:+54413378 None:intergenic
CCAAAATAGCTAGATACTAG+AGG 0.518062 7_4:+54413702 None:intergenic
TCTGCTTCATCCTAAAACAA+TGG 0.518966 7_4:+54412176 None:intergenic
GAGAGAACTATTGTTGGTTG+TGG 0.537065 7_4:+54413419 None:intergenic
AATGGAGAAAATATAGGAGA+TGG 0.552366 7_4:+54413144 None:intergenic
AACAAAACCTTCTCATCAAG+AGG 0.557222 7_4:-54413291 Msa1163270:CDS
AAGTACATAAACATTCCAAA+AGG 0.561300 7_4:-54413793 Msa1163270:five_prime_UTR
TTCATCAAATTCCGTAAGTG+TGG 0.570593 7_4:-54412423 Msa1163270:three_prime_UTR
GGATATAGAGATAAGAGAGA+GGG 0.577792 7_4:-54412254 Msa1163270:three_prime_UTR
TTGGTGAACCAAATAAAGAA+GGG 0.578160 7_4:+54413463 None:intergenic
CAAAATAGCTAGATACTAGA+GGG 0.581790 7_4:+54413703 None:intergenic
CTTTCTGCAGTAGAGAATGC+AGG 0.590215 7_4:-54412652 Msa1163270:CDS
TGGATAACCAAAACCATAAG+GGG 0.594533 7_4:+54413347 None:intergenic
TTTGATTTATCATCTATGCA+AGG 0.594681 7_4:+54413753 None:intergenic
TGGATATAGAGATAAGAGAG+AGG 0.618666 7_4:-54412255 Msa1163270:three_prime_UTR
AATTCTGTTGAACAAAACAA+AGG 0.636708 7_4:-54413213 Msa1163270:CDS
GCATAGATGATAAATCAAAG+TGG 0.653145 7_4:-54413749 Msa1163270:five_prime_UTR
GTGCATGTTTAGATCAACCG+TGG 0.655998 7_4:+54412389 None:intergenic
GAGTGAGATGGAGCTTTGTG+AGG 0.659407 7_4:-54413070 Msa1163270:CDS
GTGTGGTTCGGTTAAAACCA+CGG 0.660104 7_4:-54412406 Msa1163270:three_prime_UTR
GCAATTATCGAATATATGAG+TGG 0.666245 7_4:+54413008 None:intergenic
TGCTTCATCCTAAAACAATG+GGG 0.668770 7_4:+54412178 None:intergenic
GCTTCATCCTAAAACAATGG+GGG 0.681227 7_4:+54412179 None:intergenic
CTAGTCATGATATCATAACG+AGG 0.716962 7_4:+54412504 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAGATATATTTATTTAT+AGG - chr7_4:54413057-54413076 Msa1163270:CDS 10.0%
!! CAAAAGATATATTTATTTAT+AGG - chr7_4:54413057-54413076 Msa1163270:CDS 10.0%
!!! TTGAAATCTCTAATAAATTT+TGG + chr7_4:54412498-54412517 None:intergenic 15.0%
!!! TTTATTTCTATTTTTCCTTT+TGG - chr7_4:54413627-54413646 Msa1163270:intron 15.0%
!!! TTGAAATCTCTAATAAATTT+TGG + chr7_4:54412498-54412517 None:intergenic 15.0%
!!! TTTATTTCTATTTTTCCTTT+TGG - chr7_4:54413627-54413646 Msa1163270:intron 15.0%
!!! TTTAGCACAATTTTGATTAT+TGG + chr7_4:54412448-54412467 None:intergenic 20.0%
!!! ATTTTGTTGTCATAAAAAAG+TGG + chr7_4:54412615-54412634 None:intergenic 20.0%
!!! TTTAGCACAATTTTGATTAT+TGG + chr7_4:54412448-54412467 None:intergenic 20.0%
!!! ATTTTGTTGTCATAAAAAAG+TGG + chr7_4:54412615-54412634 None:intergenic 20.0%
!! TTTGATTTATCATCTATGCA+AGG + chr7_4:54412189-54412208 None:intergenic 25.0%
! GATAAATCAAAGTGGTTTAT+TGG - chr7_4:54412198-54412217 Msa1163270:three_prime_UTR 25.0%
!! TGCAGATGATTTTTCTTTTT+GGG - chr7_4:54412340-54412359 Msa1163270:three_prime_UTR 25.0%
! AAAACTATCCCTTCTTTATT+TGG - chr7_4:54412468-54412487 Msa1163270:three_prime_UTR 25.0%
! AATTCTGTTGAACAAAACAA+AGG - chr7_4:54412726-54412745 Msa1163270:intron 25.0%
!!! AACAAAGGAAATGTTTTGTT+TGG - chr7_4:54412741-54412760 Msa1163270:intron 25.0%
!!! ATTTTGTGTATCTTTGGTTT+TGG + chr7_4:54412832-54412851 None:intergenic 25.0%
!!! TTTGGAATTTTGTGTATCTT+TGG + chr7_4:54412838-54412857 None:intergenic 25.0%
! AACAATTCTAACTCATCTTA+TGG - chr7_4:54412987-54413006 Msa1163270:CDS 25.0%
!! TCATATAACTAGATTTTTGC+TGG - chr7_4:54413210-54413229 Msa1163270:CDS 25.0%
!!! TTTCTATTTTTCCTTTTGGA+AGG - chr7_4:54413631-54413650 Msa1163270:intron 25.0%
!!! TTCTATTTTTCCTTTTGGAA+GGG - chr7_4:54413632-54413651 Msa1163270:intron 25.0%
!! TTTGATTTATCATCTATGCA+AGG + chr7_4:54412189-54412208 None:intergenic 25.0%
! GATAAATCAAAGTGGTTTAT+TGG - chr7_4:54412198-54412217 Msa1163270:three_prime_UTR 25.0%
!! TGCAGATGATTTTTCTTTTT+GGG - chr7_4:54412340-54412359 Msa1163270:three_prime_UTR 25.0%
! AAAACTATCCCTTCTTTATT+TGG - chr7_4:54412468-54412487 Msa1163270:three_prime_UTR 25.0%
! AATTCTGTTGAACAAAACAA+AGG - chr7_4:54412726-54412745 Msa1163270:intron 25.0%
!!! AACAAAGGAAATGTTTTGTT+TGG - chr7_4:54412741-54412760 Msa1163270:intron 25.0%
!!! ATTTTGTGTATCTTTGGTTT+TGG + chr7_4:54412832-54412851 None:intergenic 25.0%
!!! TTTGGAATTTTGTGTATCTT+TGG + chr7_4:54412838-54412857 None:intergenic 25.0%
! AACAATTCTAACTCATCTTA+TGG - chr7_4:54412987-54413006 Msa1163270:CDS 25.0%
!! TCATATAACTAGATTTTTGC+TGG - chr7_4:54413210-54413229 Msa1163270:CDS 25.0%
!!! TTTCTATTTTTCCTTTTGGA+AGG - chr7_4:54413631-54413650 Msa1163270:intron 25.0%
!!! TTCTATTTTTCCTTTTGGAA+GGG - chr7_4:54413632-54413651 Msa1163270:intron 25.0%
GCATAGATGATAAATCAAAG+TGG - chr7_4:54412190-54412209 Msa1163270:three_prime_UTR 30.0%
CAAAATAGCTAGATACTAGA+GGG + chr7_4:54412239-54412258 None:intergenic 30.0%
!! CTCTAGTATCTAGCTATTTT+GGG - chr7_4:54412238-54412257 Msa1163270:three_prime_UTR 30.0%
! GTGCAGATGATTTTTCTTTT+TGG - chr7_4:54412339-54412358 Msa1163270:three_prime_UTR 30.0%
TTGGTGAACCAAATAAAGAA+GGG + chr7_4:54412479-54412498 None:intergenic 30.0%
TTTGGTGAACCAAATAAAGA+AGG + chr7_4:54412480-54412499 None:intergenic 30.0%
TAGTGTCAAGAATTGAAGTT+GGG + chr7_4:54412563-54412582 None:intergenic 30.0%
CAATTGAATCATCATCCTTA+AGG + chr7_4:54412698-54412717 None:intergenic 30.0%
AATGGAGAAAATATAGGAGA+TGG + chr7_4:54412798-54412817 None:intergenic 30.0%
GACTCAAATGGAGAAAATAT+AGG + chr7_4:54412804-54412823 None:intergenic 30.0%
GCAATTATCGAATATATGAG+TGG + chr7_4:54412934-54412953 None:intergenic 30.0%
!! GATGAGTTAGAATTGTTTTG+TGG + chr7_4:54412984-54413003 None:intergenic 30.0%
AGTACTAATGCTTATTCTTG+AGG - chr7_4:54413359-54413378 Msa1163270:CDS 30.0%
! TAGAGTGCATATTTGAAATC+AGG - chr7_4:54413455-54413474 Msa1163270:CDS 30.0%
AACAAAAAAACCCTTCCAAA+AGG + chr7_4:54413645-54413664 None:intergenic 30.0%
TCTCTTATCTCTATATCCAT+TGG + chr7_4:54413683-54413702 None:intergenic 30.0%
CCAAATTAAGAGTCATTACT+TGG + chr7_4:54413724-54413743 None:intergenic 30.0%
CCAAGTAATGACTCTTAATT+TGG - chr7_4:54413721-54413740 Msa1163270:five_prime_UTR 30.0%
GCATAGATGATAAATCAAAG+TGG - chr7_4:54412190-54412209 Msa1163270:three_prime_UTR 30.0%
CAAAATAGCTAGATACTAGA+GGG + chr7_4:54412239-54412258 None:intergenic 30.0%
!! CTCTAGTATCTAGCTATTTT+GGG - chr7_4:54412238-54412257 Msa1163270:three_prime_UTR 30.0%
! GTGCAGATGATTTTTCTTTT+TGG - chr7_4:54412339-54412358 Msa1163270:three_prime_UTR 30.0%
TTGGTGAACCAAATAAAGAA+GGG + chr7_4:54412479-54412498 None:intergenic 30.0%
TTTGGTGAACCAAATAAAGA+AGG + chr7_4:54412480-54412499 None:intergenic 30.0%
TAGTGTCAAGAATTGAAGTT+GGG + chr7_4:54412563-54412582 None:intergenic 30.0%
CAATTGAATCATCATCCTTA+AGG + chr7_4:54412698-54412717 None:intergenic 30.0%
AATGGAGAAAATATAGGAGA+TGG + chr7_4:54412798-54412817 None:intergenic 30.0%
GACTCAAATGGAGAAAATAT+AGG + chr7_4:54412804-54412823 None:intergenic 30.0%
GCAATTATCGAATATATGAG+TGG + chr7_4:54412934-54412953 None:intergenic 30.0%
!! GATGAGTTAGAATTGTTTTG+TGG + chr7_4:54412984-54413003 None:intergenic 30.0%
AGTACTAATGCTTATTCTTG+AGG - chr7_4:54413359-54413378 Msa1163270:CDS 30.0%
! TAGAGTGCATATTTGAAATC+AGG - chr7_4:54413455-54413474 Msa1163270:CDS 30.0%
AACAAAAAAACCCTTCCAAA+AGG + chr7_4:54413645-54413664 None:intergenic 30.0%
TCTCTTATCTCTATATCCAT+TGG + chr7_4:54413683-54413702 None:intergenic 30.0%
CCAAATTAAGAGTCATTACT+TGG + chr7_4:54413724-54413743 None:intergenic 30.0%
CCAAGTAATGACTCTTAATT+TGG - chr7_4:54413721-54413740 Msa1163270:five_prime_UTR 30.0%
! CCTATAATAGTCAAGCCTTT+TGG + chr7_4:54412164-54412183 None:intergenic 35.0%
CCAAAAGGCTTGACTATTAT+AGG - chr7_4:54412161-54412180 Msa1163270:three_prime_UTR 35.0%
CCAAAATAGCTAGATACTAG+AGG + chr7_4:54412240-54412259 None:intergenic 35.0%
! CCTCTAGTATCTAGCTATTT+TGG - chr7_4:54412237-54412256 Msa1163270:three_prime_UTR 35.0%
AGTGACCAAGCCAAATTTAA+TGG - chr7_4:54412398-54412417 Msa1163270:three_prime_UTR 35.0%
ATCAGCCATTAAATTTGGCT+TGG + chr7_4:54412406-54412425 None:intergenic 35.0%
CTGTGATCAGCCATTAAATT+TGG + chr7_4:54412411-54412430 None:intergenic 35.0%
! TTCTGAGAGAGAACTATTGT+TGG + chr7_4:54412529-54412548 None:intergenic 35.0%
CTAGTGTCAAGAATTGAAGT+TGG + chr7_4:54412564-54412583 None:intergenic 35.0%
ACTAGAATGTTATCCCCTTA+TGG - chr7_4:54412579-54412598 Msa1163270:three_prime_UTR 35.0%
! ATGTTATCCCCTTATGGTTT+TGG - chr7_4:54412585-54412604 Msa1163270:exon 35.0%
TGGATAACCAAAACCATAAG+GGG + chr7_4:54412595-54412614 None:intergenic 35.0%
GTGGATAACCAAAACCATAA+GGG + chr7_4:54412596-54412615 None:intergenic 35.0%
AGTGGATAACCAAAACCATA+AGG + chr7_4:54412597-54412616 None:intergenic 35.0%
!!! AGGTTTTGTTTGGAACAGTT+TGG + chr7_4:54412638-54412657 None:intergenic 35.0%
!!! CTTGATGAGAAGGTTTTGTT+TGG + chr7_4:54412648-54412667 None:intergenic 35.0%
AACAAAACCTTCTCATCAAG+AGG - chr7_4:54412648-54412667 Msa1163270:CDS 35.0%
TGCTCTTATAGGTAACCTTA+AGG - chr7_4:54412680-54412699 Msa1163270:intron 35.0%
AATATAGGAGATGGCAAAGT+TGG + chr7_4:54412789-54412808 None:intergenic 35.0%
!!! GTTTTGGTTTGAGACTCAAA+TGG + chr7_4:54412816-54412835 None:intergenic 35.0%
CTCACTCAAAGAAAGAAGTT+TGG + chr7_4:54412856-54412875 None:intergenic 35.0%
ACTTCTTTCTTTGAGTGAGA+TGG - chr7_4:54412857-54412876 Msa1163270:intron 35.0%
TATACTTGTGTGATCTCTCA+TGG - chr7_4:54412894-54412913 Msa1163270:CDS 35.0%
ATATGAGTGGTTCTTGGATT+AGG + chr7_4:54412921-54412940 None:intergenic 35.0%
TCGAATATATGAGTGGTTCT+TGG + chr7_4:54412927-54412946 None:intergenic 35.0%
! AGTTTGATTGTGTTTTGCAG+AGG - chr7_4:54413256-54413275 Msa1163270:CDS 35.0%
CTAGTCATGATATCATAACG+AGG + chr7_4:54413438-54413457 None:intergenic 35.0%
TTCATCAAATTCCGTAAGTG+TGG - chr7_4:54413516-54413535 Msa1163270:CDS 35.0%
! TTTTAACCGAACCACACTTA+CGG + chr7_4:54413530-54413549 None:intergenic 35.0%
TGGATATAGAGATAAGAGAG+AGG - chr7_4:54413684-54413703 Msa1163270:intron 35.0%
GGATATAGAGATAAGAGAGA+GGG - chr7_4:54413685-54413704 Msa1163270:intron 35.0%
! ACTCTTAATTTGGTTGCTTC+TGG - chr7_4:54413731-54413750 Msa1163270:five_prime_UTR 35.0%
TGCTTCATCCTAAAACAATG+GGG + chr7_4:54413764-54413783 None:intergenic 35.0%
CTGCTTCATCCTAAAACAAT+GGG + chr7_4:54413765-54413784 None:intergenic 35.0%
TCTGCTTCATCCTAAAACAA+TGG + chr7_4:54413766-54413785 None:intergenic 35.0%
! CCTATAATAGTCAAGCCTTT+TGG + chr7_4:54412164-54412183 None:intergenic 35.0%
CCAAAAGGCTTGACTATTAT+AGG - chr7_4:54412161-54412180 Msa1163270:three_prime_UTR 35.0%
CCAAAATAGCTAGATACTAG+AGG + chr7_4:54412240-54412259 None:intergenic 35.0%
! CCTCTAGTATCTAGCTATTT+TGG - chr7_4:54412237-54412256 Msa1163270:three_prime_UTR 35.0%
AGTGACCAAGCCAAATTTAA+TGG - chr7_4:54412398-54412417 Msa1163270:three_prime_UTR 35.0%
ATCAGCCATTAAATTTGGCT+TGG + chr7_4:54412406-54412425 None:intergenic 35.0%
CTGTGATCAGCCATTAAATT+TGG + chr7_4:54412411-54412430 None:intergenic 35.0%
! TTCTGAGAGAGAACTATTGT+TGG + chr7_4:54412529-54412548 None:intergenic 35.0%
CTAGTGTCAAGAATTGAAGT+TGG + chr7_4:54412564-54412583 None:intergenic 35.0%
ACTAGAATGTTATCCCCTTA+TGG - chr7_4:54412579-54412598 Msa1163270:three_prime_UTR 35.0%
! ATGTTATCCCCTTATGGTTT+TGG - chr7_4:54412585-54412604 Msa1163270:exon 35.0%
TGGATAACCAAAACCATAAG+GGG + chr7_4:54412595-54412614 None:intergenic 35.0%
GTGGATAACCAAAACCATAA+GGG + chr7_4:54412596-54412615 None:intergenic 35.0%
AGTGGATAACCAAAACCATA+AGG + chr7_4:54412597-54412616 None:intergenic 35.0%
!!! AGGTTTTGTTTGGAACAGTT+TGG + chr7_4:54412638-54412657 None:intergenic 35.0%
!!! CTTGATGAGAAGGTTTTGTT+TGG + chr7_4:54412648-54412667 None:intergenic 35.0%
AACAAAACCTTCTCATCAAG+AGG - chr7_4:54412648-54412667 Msa1163270:CDS 35.0%
TGCTCTTATAGGTAACCTTA+AGG - chr7_4:54412680-54412699 Msa1163270:intron 35.0%
AATATAGGAGATGGCAAAGT+TGG + chr7_4:54412789-54412808 None:intergenic 35.0%
!!! GTTTTGGTTTGAGACTCAAA+TGG + chr7_4:54412816-54412835 None:intergenic 35.0%
CTCACTCAAAGAAAGAAGTT+TGG + chr7_4:54412856-54412875 None:intergenic 35.0%
ACTTCTTTCTTTGAGTGAGA+TGG - chr7_4:54412857-54412876 Msa1163270:intron 35.0%
TATACTTGTGTGATCTCTCA+TGG - chr7_4:54412894-54412913 Msa1163270:CDS 35.0%
ATATGAGTGGTTCTTGGATT+AGG + chr7_4:54412921-54412940 None:intergenic 35.0%
TCGAATATATGAGTGGTTCT+TGG + chr7_4:54412927-54412946 None:intergenic 35.0%
! AGTTTGATTGTGTTTTGCAG+AGG - chr7_4:54413256-54413275 Msa1163270:CDS 35.0%
CTAGTCATGATATCATAACG+AGG + chr7_4:54413438-54413457 None:intergenic 35.0%
TTCATCAAATTCCGTAAGTG+TGG - chr7_4:54413516-54413535 Msa1163270:CDS 35.0%
! TTTTAACCGAACCACACTTA+CGG + chr7_4:54413530-54413549 None:intergenic 35.0%
TGGATATAGAGATAAGAGAG+AGG - chr7_4:54413684-54413703 Msa1163270:intron 35.0%
GGATATAGAGATAAGAGAGA+GGG - chr7_4:54413685-54413704 Msa1163270:intron 35.0%
! ACTCTTAATTTGGTTGCTTC+TGG - chr7_4:54413731-54413750 Msa1163270:five_prime_UTR 35.0%
TGCTTCATCCTAAAACAATG+GGG + chr7_4:54413764-54413783 None:intergenic 35.0%
CTGCTTCATCCTAAAACAAT+GGG + chr7_4:54413765-54413784 None:intergenic 35.0%
TCTGCTTCATCCTAAAACAA+TGG + chr7_4:54413766-54413785 None:intergenic 35.0%
! GAGAGAACTATTGTTGGTTG+TGG + chr7_4:54412523-54412542 None:intergenic 40.0%
! CACAGAGAAATGGTGTTAGA+TGG - chr7_4:54413310-54413329 Msa1163270:CDS 40.0%
CAAATTCCGTAAGTGTGGTT+CGG - chr7_4:54413521-54413540 Msa1163270:CDS 40.0%
TTTGTCTTTGAGCTCTCCAA+TGG - chr7_4:54413664-54413683 Msa1163270:intron 40.0%
! GTTTTTGCCCCCATTGTTTT+AGG - chr7_4:54413753-54413772 Msa1163270:five_prime_UTR 40.0%
GCTTCATCCTAAAACAATGG+GGG + chr7_4:54413763-54413782 None:intergenic 40.0%
! GAGAGAACTATTGTTGGTTG+TGG + chr7_4:54412523-54412542 None:intergenic 40.0%
! CACAGAGAAATGGTGTTAGA+TGG - chr7_4:54413310-54413329 Msa1163270:CDS 40.0%
CAAATTCCGTAAGTGTGGTT+CGG - chr7_4:54413521-54413540 Msa1163270:CDS 40.0%
TTTGTCTTTGAGCTCTCCAA+TGG - chr7_4:54413664-54413683 Msa1163270:intron 40.0%
! GTTTTTGCCCCCATTGTTTT+AGG - chr7_4:54413753-54413772 Msa1163270:five_prime_UTR 40.0%
GCTTCATCCTAAAACAATGG+GGG + chr7_4:54413763-54413782 None:intergenic 40.0%
! ACCTTCTCATCAAGAGGCAT+TGG - chr7_4:54412654-54412673 Msa1163270:CDS 45.0%
ACCAATGCCTCTTGATGAGA+AGG + chr7_4:54412658-54412677 None:intergenic 45.0%
!! GGCATTGGTCTTGCTCTTAT+AGG - chr7_4:54412669-54412688 Msa1163270:intron 45.0%
CTTTCTGCAGTAGAGAATGC+AGG - chr7_4:54413287-54413306 Msa1163270:CDS 45.0%
AGAATGCAGGCACAGAGAAA+TGG - chr7_4:54413300-54413319 Msa1163270:CDS 45.0%
!! GTGTGGTTCGGTTAAAACCA+CGG - chr7_4:54413533-54413552 Msa1163270:CDS 45.0%
GTGCATGTTTAGATCAACCG+TGG + chr7_4:54413553-54413572 None:intergenic 45.0%
! ACCTTCTCATCAAGAGGCAT+TGG - chr7_4:54412654-54412673 Msa1163270:CDS 45.0%
ACCAATGCCTCTTGATGAGA+AGG + chr7_4:54412658-54412677 None:intergenic 45.0%
!! GGCATTGGTCTTGCTCTTAT+AGG - chr7_4:54412669-54412688 Msa1163270:intron 45.0%
CTTTCTGCAGTAGAGAATGC+AGG - chr7_4:54413287-54413306 Msa1163270:CDS 45.0%
AGAATGCAGGCACAGAGAAA+TGG - chr7_4:54413300-54413319 Msa1163270:CDS 45.0%
!! GTGTGGTTCGGTTAAAACCA+CGG - chr7_4:54413533-54413552 Msa1163270:CDS 45.0%
GTGCATGTTTAGATCAACCG+TGG + chr7_4:54413553-54413572 None:intergenic 45.0%
! GAGTGAGATGGAGCTTTGTG+AGG - chr7_4:54412869-54412888 Msa1163270:intron 50.0%
! GAGTGAGATGGAGCTTTGTG+AGG - chr7_4:54412869-54412888 Msa1163270:intron 50.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 54412148 54413813 54412148 ID=Msa1163270;Name=Msa1163270
chr7_4 mRNA 54412148 54413813 54412148 ID=Msa1163270-mRNA-1;Parent=Msa1163270;Name=Msa1163270-mRNA-1;_AED=0.02;_eAED=0.02;_QI=112|1|1|1|0.5|0.33|3|456|271
chr7_4 exon 54412148 54412685 54412148 ID=Msa1163270-mRNA-1:exon:15817;Parent=Msa1163270-mRNA-1
chr7_4 exon 54412883 54413616 54412883 ID=Msa1163270-mRNA-1:exon:15816;Parent=Msa1163270-mRNA-1
chr7_4 exon 54413702 54413813 54413702 ID=Msa1163270-mRNA-1:exon:15815;Parent=Msa1163270-mRNA-1
chr7_4 five_prime_UTR 54413702 54413813 54413702 ID=Msa1163270-mRNA-1:five_prime_utr;Parent=Msa1163270-mRNA-1
chr7_4 CDS 54412883 54413616 54412883 ID=Msa1163270-mRNA-1:cds;Parent=Msa1163270-mRNA-1
chr7_4 CDS 54412604 54412685 54412604 ID=Msa1163270-mRNA-1:cds;Parent=Msa1163270-mRNA-1
chr7_4 three_prime_UTR 54412148 54412603 54412148 ID=Msa1163270-mRNA-1:three_prime_utr;Parent=Msa1163270-mRNA-1
Gene Sequence

>Msa1163270

ATGATTTTTCTTTTTGGGAGAGTACATATGCTTCTAAGAAATAGATCAAGAGCAGTGACCAAGCCAAATTTAATGGCTGATCACAGTTCTCAACAATCTTCCAATAATCAAAATTGTGCTAAAAAAACTATCCCTTCTTTATTTGGTTCACCAAAATTTATTAGAGATTTCAACACCACAACCAACAATAGTTCTCTCTCAGAAGCTTTGAAAAGCCCAACTTCAATTCTTGACACTAGAATGTTATCCCCTTATGGTTTTGGTTATCCACTTTTTTATGACAACAAAATCCAAACTGTTCCAAACAAAACCTTCTCATCAAGAGGCATTGGTCTTGCTCTTATAGGTAACCTTAAGGATGATGATTCAATTGATGAGAATAATTCTGTTGAACAAAACAAAGGAAATGTTTTGTTTGGAACACAGCTTAGAGTTAAAGTACCAACTTTGCCATCTCCTATATTTTCTCCATTTGAGTCTCAAACCAAAACCAAAGATACACAAAATTCCAAACTTCTTTCTTTGAGTGAGATGGAGCTTTGTGAGGAATATACTTGTGTGATCTCTCATGGACCTAATCCAAGAACCACTCATATATTCGATAATTGCGTTGTCGAAGAGAGTTGCTGTTCTCTGCCACAAAACAATTCTAACTCATCTTATGGAAATTTTCTTAGAGTTTGTTACACTTGCAAGAAACATCTTGAGCAGACAAAAGATATATTTATTTATAGAGGAGACAAAGCTTTCTGCAGTAGAGAATGCAGGCACAGAGAAATGGTGTTAGATGGTGTAGAAAGTTTAGAAGAATTTTGA
Protein sequence

>Msa1163270

MIFLFGRVHMLLRNRSRAVTKPNLMADHSSQQSSNNQNCAKKTIPSLFGSPKFIRDFNTTTNNSSLSEALKSPTSILDTRMLSPYGFGYPLFYDNKIQTVPNKTFSSRGIGLALIGNLKDDDSIDENNSVEQNKGNVLFGTQLRVKVPTLPSPIFSPFESQTKTKDTQNSKLLSLSEMELCEEYTCVISHGPNPRTTHIFDNCVVEESCCSLPQNNSNSSYGNFLRVCYTCKKHLEQTKDIFIYRGDKAFCSRECRHREMVLDGVESLEEF*