AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1118000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1118000 MtrunA17_Chr7g0254701 98.502 267 4 0 20 286 1 267 0.0 537
Msa1118000 MtrunA17_Chr4g0060351 36.029 136 87 0 32 167 7 142 3.44e-29 108
Msa1118000 MtrunA17_Chr8g0362321 35.294 136 88 0 32 167 7 142 3.51e-29 108
Msa1118000 MtrunA17_Chr2g0316211 38.158 152 90 1 23 174 3 150 7.42e-29 107
Msa1118000 MtrunA17_Chr2g0316201 37.500 152 91 1 23 174 3 150 4.30e-28 105
Msa1118000 MtrunA17_Chr3g0131871 35.338 133 85 1 48 179 22 154 2.26e-26 102
Msa1118000 MtrunA17_Chr3g0108261 34.965 143 93 0 32 174 4 146 6.31e-25 97.4
Msa1118000 MtrunA17_Chr5g0432831 34.965 143 93 0 32 174 4 146 6.31e-25 97.4
Msa1118000 MtrunA17_Chr1g0154011 34.965 143 93 0 32 174 4 146 7.16e-25 97.1
Msa1118000 MtrunA17_Chr7g0275361 34.266 143 94 0 32 174 4 146 2.81e-24 95.5
Msa1118000 MtrunA17_Chr8g0369311 34.266 143 94 0 32 174 60 202 7.83e-24 95.9
Msa1118000 MtrunA17_Chr4g0037011 34.532 139 91 0 32 170 4 142 1.18e-23 94.0
Msa1118000 MtrunA17_Chr3g0139901 35.570 149 92 3 33 177 7 155 1.20e-23 94.4
Msa1118000 MtrunA17_Chr1g0199951 32.192 146 95 3 29 171 41 185 2.32e-18 81.3
Msa1118000 MtrunA17_Chr8g0378601 29.921 127 89 0 32 158 25 151 5.64e-18 79.3
Msa1118000 MtrunA17_Chr4g0053191 35.849 106 66 1 65 168 45 150 7.43e-18 79.0
Msa1118000 MtrunA17_Chr4g0038891 29.518 166 109 2 11 169 7 171 1.98e-17 78.2
Msa1118000 MtrunA17_Chr5g0396451 31.915 141 95 1 29 169 32 171 9.48e-17 76.3
Msa1118000 MtrunA17_Chr5g0401831 30.986 142 91 3 33 167 8 149 9.69e-17 75.9
Msa1118000 MtrunA17_Chr8g0348701 27.344 128 91 2 50 175 21 148 3.15e-16 75.1
Msa1118000 MtrunA17_Chr8g0387131 28.125 128 90 2 50 175 21 148 6.39e-16 74.3
Msa1118000 MtrunA17_Chr4g0059291 25.600 125 91 2 55 177 26 150 1.21e-12 65.1
Msa1118000 MtrunA17_Chr5g0403591 33.000 100 67 0 68 167 67 166 2.32e-12 64.3
Msa1118000 MtrunA17_Chr4g0031181 27.928 111 67 1 67 164 43 153 3.15e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1118000 AT5G05080.2 68.539 267 68 2 20 286 1 251 2.34e-128 365
Msa1118000 AT5G05080.3 68.539 267 68 2 20 286 1 251 2.34e-128 365
Msa1118000 AT5G05080.1 68.539 267 68 2 20 286 1 251 2.34e-128 365
Msa1118000 AT5G05080.4 68.539 267 68 2 20 286 1 251 2.34e-128 365
Msa1118000 AT2G02760.1 35.294 136 88 0 32 167 7 142 1.06e-28 107
Msa1118000 AT2G02760.2 35.294 136 88 0 32 167 7 142 1.06e-28 107
Msa1118000 AT2G02760.4 35.294 136 88 0 32 167 7 142 1.06e-28 107
Msa1118000 AT2G02760.3 35.294 136 88 0 32 167 7 142 1.06e-28 107
Msa1118000 AT1G14400.1 35.294 136 88 0 32 167 7 142 1.67e-28 107
Msa1118000 AT1G14400.2 35.294 136 88 0 32 167 7 142 1.67e-28 107
Msa1118000 AT1G78870.2 37.500 152 91 1 23 174 3 150 9.07e-28 105
Msa1118000 AT1G16890.2 36.842 152 92 1 23 174 3 150 2.84e-27 103
Msa1118000 AT5G50870.1 33.793 145 94 2 33 175 6 150 2.56e-26 102
Msa1118000 AT5G62540.1 35.338 133 86 0 32 164 7 139 4.34e-26 100
Msa1118000 AT1G16890.3 34.591 159 95 2 23 174 3 159 2.90e-25 99.0
Msa1118000 AT4G27960.1 34.266 143 94 0 32 174 4 146 1.03e-24 97.1
Msa1118000 AT5G41700.5 34.965 143 93 0 32 174 4 146 1.09e-24 97.1
Msa1118000 AT5G41700.2 34.965 143 93 0 32 174 4 146 1.09e-24 97.1
Msa1118000 AT5G41700.1 34.965 143 93 0 32 174 4 146 1.09e-24 97.1
Msa1118000 AT5G53300.2 34.266 143 94 0 32 174 4 146 1.80e-24 96.7
Msa1118000 AT5G53300.4 34.266 143 94 0 32 174 4 146 1.80e-24 96.7
Msa1118000 AT5G53300.1 34.266 143 94 0 32 174 4 146 1.80e-24 96.7
Msa1118000 AT1G64230.2 33.566 143 95 0 32 174 4 146 2.86e-24 95.9
Msa1118000 AT1G64230.1 33.566 143 95 0 32 174 4 146 2.86e-24 95.9
Msa1118000 AT2G16740.1 34.965 143 93 0 32 174 4 146 5.31e-24 95.1
Msa1118000 AT3G08690.2 33.566 143 95 0 32 174 4 146 7.83e-24 94.7
Msa1118000 AT3G08690.1 33.566 143 95 0 32 174 4 146 7.83e-24 94.7
Msa1118000 AT5G41700.4 35.862 145 90 2 32 174 4 147 1.36e-23 94.4
Msa1118000 AT5G56150.1 32.867 143 96 0 32 174 4 146 1.91e-23 94.0
Msa1118000 AT1G78870.3 40.909 110 61 1 23 132 3 108 2.43e-23 92.4
Msa1118000 AT4G27960.2 34.266 143 94 0 32 174 34 176 2.81e-23 94.4
Msa1118000 AT1G64230.3 32.895 152 93 2 32 174 4 155 3.27e-23 93.6
Msa1118000 AT5G50870.2 31.410 156 94 3 33 175 6 161 3.42e-23 94.7
Msa1118000 AT1G16890.1 40.909 110 65 0 65 174 8 117 4.81e-23 92.0
Msa1118000 AT1G78870.4 40.909 110 65 0 65 174 8 117 6.87e-23 91.7
Msa1118000 AT3G24515.1 37.342 158 86 3 33 177 10 167 1.51e-22 96.7
Msa1118000 AT5G25760.1 37.302 126 76 2 33 155 7 132 2.90e-22 90.9
Msa1118000 AT5G25760.2 37.302 126 76 2 33 155 7 132 2.90e-22 90.9
Msa1118000 AT3G13550.1 30.556 144 100 0 32 175 39 182 8.12e-22 90.5
Msa1118000 AT1G36340.2 40.351 114 68 0 63 176 41 154 1.31e-21 89.0
Msa1118000 AT1G36340.1 40.351 114 68 0 63 176 41 154 1.31e-21 89.0
Msa1118000 AT3G08700.2 31.944 144 97 1 32 174 4 147 2.32e-21 88.2
Msa1118000 AT3G08700.1 31.944 144 97 1 32 174 4 147 2.32e-21 88.2
Msa1118000 AT1G78870.1 40.541 111 65 1 65 174 8 118 4.14e-21 86.7
Msa1118000 AT5G41700.3 37.500 104 65 0 32 135 4 107 4.43e-21 87.4
Msa1118000 AT3G24515.3 36.842 133 77 2 49 177 63 192 2.31e-20 90.9
Msa1118000 AT3G13550.2 32.143 112 76 0 32 143 39 150 9.38e-20 84.7
Msa1118000 AT5G56150.2 35.849 106 68 0 32 137 4 109 1.13e-19 82.8
Msa1118000 AT5G53300.3 36.792 106 66 1 32 136 4 109 1.24e-19 82.4
Msa1118000 AT1G64230.5 35.714 112 72 0 63 174 77 188 1.37e-19 84.7
Msa1118000 AT1G64230.4 35.714 112 72 0 63 174 77 188 1.37e-19 84.7
Msa1118000 AT3G20060.1 33.571 140 92 1 30 169 37 175 6.42e-18 80.1
Msa1118000 AT1G50490.1 34.043 141 92 1 29 169 35 174 3.19e-17 78.2
Msa1118000 AT3G20060.2 34.426 122 79 1 48 169 10 130 5.44e-16 73.6
Msa1118000 AT1G63800.1 28.462 130 91 2 50 177 21 150 5.66e-16 74.7
Msa1118000 AT1G63800.2 28.462 130 91 2 50 177 6 135 5.90e-16 74.3
Msa1118000 AT3G57870.1 30.070 143 93 3 33 168 9 151 1.62e-15 72.8
Msa1118000 AT5G41340.1 27.344 128 91 2 50 175 21 148 1.15e-14 71.2
Msa1118000 AT2G46030.5 28.125 128 90 2 50 175 21 148 5.30e-14 69.3
Msa1118000 AT2G46030.1 28.125 128 90 2 50 175 21 148 5.30e-14 69.3
Msa1118000 AT2G18600.1 33.043 115 74 1 68 179 69 183 1.14e-13 68.6
Msa1118000 AT2G18600.2 33.043 115 74 1 68 179 69 183 1.14e-13 68.6
Msa1118000 AT2G46030.4 28.125 128 90 2 50 175 24 151 1.27e-13 68.2
Msa1118000 AT2G46030.2 28.125 128 90 2 50 175 24 151 1.27e-13 68.2
Msa1118000 AT2G46030.3 28.125 128 90 2 50 175 93 220 2.89e-13 68.6
Msa1118000 AT4G36800.2 30.986 142 92 3 33 171 34 172 3.10e-13 67.0
Msa1118000 AT4G36800.1 30.986 142 92 3 33 171 34 172 3.10e-13 67.0
Msa1118000 AT5G41340.2 28.571 105 73 2 73 175 9 113 8.12e-12 62.4
Msa1118000 AT2G32790.1 35.398 113 73 0 63 175 64 176 1.69e-11 62.0

Find 76 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCACTGTTGGCAATATT+TGG 0.133789 7_3:+66547838 None:intergenic
TTGTGTACTATTGATCTTTC+TGG 0.148256 7_3:-66546806 Msa1118000:three_prime_UTR
AAAACTAAGGCACAAATAAA+AGG 0.283841 7_3:+66546918 None:intergenic
GTCATTTACTGTTGATTACT+TGG 0.327343 7_3:-66546708 Msa1118000:three_prime_UTR
AGTTCATTTAAAGCTGATTC+TGG 0.328582 7_3:+66547606 None:intergenic
TTGTGTCAACGCGCTCAAAA+AGG 0.346414 7_3:-66547812 Msa1118000:CDS
TCCTCTTGCTCCAGTGGTTG+AGG 0.353319 7_3:+66547139 None:intergenic
CCCATAATCAACAGGTTGTT+AGG 0.362147 7_3:-66547650 Msa1118000:intron
AGAATCAGCTTTAAATGAAC+TGG 0.367979 7_3:-66547604 Msa1118000:CDS
GCAAGTTGCCTTATCACATT+AGG 0.371102 7_3:+66548386 None:intergenic
TTGCAATTTGATAGAAAGAA+AGG 0.378430 7_3:-66546897 Msa1118000:three_prime_UTR
TGTGTACTATTGATCTTTCT+GGG 0.394804 7_3:-66546805 Msa1118000:three_prime_UTR
TCTATCAAATTGCAAAACTA+AGG 0.411937 7_3:+66546905 None:intergenic
CCTCTTGCTCCAGTGGTTGA+GGG 0.414800 7_3:+66547140 None:intergenic
AAGTGTTTGAGTAAAATTAG+AGG 0.432184 7_3:+66546685 None:intergenic
ATTCAGAGCAGTAGTTGATT+CGG 0.434687 7_3:+66547244 None:intergenic
TTTAAAGCTGATTCTGGAAA+CGG 0.436344 7_3:+66547612 None:intergenic
AATATTTGGATGGAAAATCT+TGG 0.449656 7_3:+66547852 None:intergenic
GGAATCCTAGCCTTGGGTTA+CGG 0.454102 7_3:-66547786 Msa1118000:CDS
GGGGAAGGGATATGGGTAAT+GGG 0.460053 7_3:-66546842 Msa1118000:three_prime_UTR
AAGAGGAACTAGTCAGGATC+AGG 0.466133 7_3:-66547123 Msa1118000:CDS
AGGATTGGAATCCTAGCCTT+GGG 0.467861 7_3:-66547792 Msa1118000:CDS
CGGAAAACTCCACTCTCATA+AGG 0.469183 7_3:+66548170 None:intergenic
CTGTCATTGCCCTCAACCAC+TGG 0.475571 7_3:-66547149 Msa1118000:CDS
TTTCCACACTCTCCTCCTAA+AGG 0.481533 7_3:-66548122 Msa1118000:intron
GACATCAAAACCATGCCGTC+AGG 0.481655 7_3:-66547188 Msa1118000:CDS
ATCTTCCGCCTAATGTGATA+AGG 0.491201 7_3:-66548394 Msa1118000:CDS
CTGCTCATGCACCACAAAAG+AGG 0.495555 7_3:-66547037 Msa1118000:CDS
ATCAACAGTAAATGACAGTA+AGG 0.500543 7_3:+66546715 None:intergenic
AACTTTAATGCCCTCAGGAG+GGG 0.501972 7_3:+66548337 None:intergenic
ACTAGTTCCTCTTGCTCCAG+TGG 0.503278 7_3:+66547133 None:intergenic
AAGGATTGGAATCCTAGCCT+TGG 0.504209 7_3:-66547793 Msa1118000:CDS
CACTGTTGGCAATATTTGGA+TGG 0.511422 7_3:+66547842 None:intergenic
GACACAAATCTCTCCACTGT+TGG 0.513774 7_3:+66547828 None:intergenic
TTTGCTGACATTGAAGGCCC+TGG 0.514631 7_3:-66548284 Msa1118000:intron
ACTACTGCTTGAAAATTATG+AGG 0.514928 7_3:-66547574 Msa1118000:CDS
TTCGGAAGCTCCTCACTCCT+CGG 0.528489 7_3:-66548882 Msa1118000:intron
TATTTAAACACACCTTTAGG+AGG 0.530844 7_3:+66548110 None:intergenic
TGGAGCAAGAGGAACTAGTC+AGG 0.535304 7_3:-66547129 Msa1118000:CDS
CATTGGAACTGCTAAAGCTC+AGG 0.536497 7_3:-66547012 Msa1118000:CDS
TCAAAGCCGAAATTCAAAAG+TGG 0.539030 7_3:-66547275 Msa1118000:CDS
TAGTCAGGATCAGGCAGTAG+TGG 0.540085 7_3:-66547114 Msa1118000:CDS
CCCTCAACCACTGGAGCAAG+AGG 0.542063 7_3:-66547140 Msa1118000:CDS
CAGAGCAGCACCTGACGGCA+TGG 0.548312 7_3:+66547178 None:intergenic
ACGACAACTTTAATGCCCTC+AGG 0.561456 7_3:+66548332 None:intergenic
GCACCACAAAAGAGGGACAT+TGG 0.563104 7_3:-66547029 Msa1118000:CDS
TGCTCATGCACCACAAAAGA+GGG 0.567894 7_3:-66547036 Msa1118000:CDS
TAATGTTTCTTCTGCTGCCG+CGG 0.572323 7_3:-66547069 Msa1118000:CDS
TCAGCTTTAAATGAACTGGC+TGG 0.575529 7_3:-66547600 Msa1118000:CDS
TGTGATAAGGCAACTTGCCA+AGG 0.579080 7_3:-66548381 Msa1118000:CDS
AGGAGTATGCTAGACATGCC+AGG 0.585519 7_3:-66547554 Msa1118000:intron
TACTTACCATCAGCAGCACA+AGG 0.588502 7_3:+66548834 None:intergenic
GGGTTCCCTTGTGCTGCTGA+TGG 0.593752 7_3:-66548840 Msa1118000:intron
TCTTGATGAAACCCCTCCTG+AGG 0.594622 7_3:-66548348 Msa1118000:CDS
AGTCAGGATCAGGCAGTAGT+GGG 0.598334 7_3:-66547113 Msa1118000:CDS
CAACTTTAATGCCCTCAGGA+GGG 0.602990 7_3:+66548336 None:intergenic
CATCCAAATATTGCCAACAG+TGG 0.613849 7_3:-66547841 Msa1118000:CDS
CTTGATGAAACCCCTCCTGA+GGG 0.614249 7_3:-66548347 Msa1118000:CDS
GCGATAGTTTCAACTTCATG+CGG 0.617025 7_3:+66548150 None:intergenic
AGTTCTGTCACCGAGGAGTG+AGG 0.617389 7_3:+66548872 None:intergenic
AGTTGCCTTATCACATTAGG+CGG 0.618457 7_3:+66548389 None:intergenic
GCTGCAACTCCTTATGAGAG+TGG 0.619407 7_3:-66548179 Msa1118000:CDS
TAAGAATATGCCGTAACCCA+AGG 0.622851 7_3:+66547776 None:intergenic
ACAACTTTAATGCCCTCAGG+AGG 0.632908 7_3:+66548335 None:intergenic
GGTGGCAGAGCAGCACCTGA+CGG 0.636975 7_3:+66547173 None:intergenic
ACACCTTTAGGAGGAGAGTG+TGG 0.641291 7_3:+66548119 None:intergenic
AGAAACATTAGCAGACGACT+CGG 0.654224 7_3:+66547082 None:intergenic
GTGACAGAACTAAACAAAGA+GGG 0.657227 7_3:-66548860 Msa1118000:CDS
GGTGACAGAACTAAACAAAG+AGG 0.668525 7_3:-66548861 Msa1118000:CDS
TTTGTTTAGTTCTGTCACCG+AGG 0.698145 7_3:+66548865 None:intergenic
GTGGTTGAGGGCAATGACAG+TGG 0.706158 7_3:+66547152 None:intergenic
ACTTACCATCAGCAGCACAA+GGG 0.715502 7_3:+66548835 None:intergenic
GTTGAGGGCAATGACAGTGG+TGG 0.726469 7_3:+66547155 None:intergenic
TGTTGATCAGACAAACACCT+CGG 0.734036 7_3:-66547222 Msa1118000:CDS
ATGAGCAGCAGAAACTACCG+CGG 0.757246 7_3:+66547052 None:intergenic
GATGTCAGCATTAAGCACCG+AGG 0.759983 7_3:+66547205 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATAATAAAT+TGG + chr7_3:66548583-66548602 None:intergenic 0.0%
!!! TTTTCTTATATTTATTCTTT+TGG - chr7_3:66546903-66546922 Msa1118000:three_prime_UTR 10.0%
!! AAACAAAAAAAAAAAAAAAG+AGG - chr7_3:66549214-66549233 Msa1118000:intron 10.0%
!! AACAAAAAAAAAAAAAAAGA+GGG - chr7_3:66549215-66549234 Msa1118000:intron 10.0%
!! TATATATATAATAAATTGGC+AGG + chr7_3:66548579-66548598 None:intergenic 15.0%
!! ACAAAAAAAAAAAAAAAGAG+GGG - chr7_3:66549216-66549235 Msa1118000:intron 15.0%
!!! ATTTTCGAACTTTTGTTATA+TGG - chr7_3:66546975-66546994 Msa1118000:CDS 20.0%
!! TAATCTGATGAAATGATATT+AGG - chr7_3:66547101-66547120 Msa1118000:CDS 20.0%
!! ATACTAAAATCTGAAGTACA+AGG + chr7_3:66547047-66547066 None:intergenic 25.0%
!!! TTGTACTTCAGATTTTAGTA+TGG - chr7_3:66547046-66547065 Msa1118000:CDS 25.0%
! GATTATCATTCAACAATCAT+GGG + chr7_3:66547086-66547105 None:intergenic 25.0%
! AGATTATCATTCAACAATCA+TGG + chr7_3:66547087-66547106 None:intergenic 25.0%
! TGATTCGTTATATCTTGTAA+CGG - chr7_3:66547287-66547306 Msa1118000:CDS 25.0%
! TTCTATTTAAACACACCTTT+AGG + chr7_3:66547980-66547999 None:intergenic 25.0%
!! CTACATTAGTATTTTTCTGT+TGG - chr7_3:66548000-66548019 Msa1118000:intron 25.0%
! CTTTCAATTCGGAAAAATAA+AGG + chr7_3:66548103-66548122 None:intergenic 25.0%
! TATACTCGATTCTTTCAATT+CGG + chr7_3:66548114-66548133 None:intergenic 25.0%
! CCTATAACATTAAATTTGAC+AGG + chr7_3:66548201-66548220 None:intergenic 25.0%
! CCTGTCAAATTTAATGTTAT+AGG - chr7_3:66548198-66548217 Msa1118000:intron 25.0%
! AATATTTGGATGGAAAATCT+TGG + chr7_3:66548235-66548254 None:intergenic 25.0%
!! TGACTTTTTGAATTAAGTTC+TGG - chr7_3:66548377-66548396 Msa1118000:CDS 25.0%
! TATATATATATGTCGATCTG+TGG - chr7_3:66548659-66548678 Msa1118000:intron 25.0%
! AAAACTAAGGCACAAATAAA+AGG + chr7_3:66549169-66549188 None:intergenic 25.0%
! TCTATCAAATTGCAAAACTA+AGG + chr7_3:66549182-66549201 None:intergenic 25.0%
! TTGCAATTTGATAGAAAGAA+AGG - chr7_3:66549187-66549206 Msa1118000:intron 25.0%
! AAAAAAAAAAAAAAGAGGGG+AGG - chr7_3:66549219-66549238 Msa1118000:intron 25.0%
! AAAAAAAAAAAAAGAGGGGA+GGG - chr7_3:66549220-66549239 Msa1118000:intron 25.0%
! AAGTGTTTGAGTAAAATTAG+AGG + chr7_3:66549402-66549421 None:intergenic 25.0%
!!! TTTTTTGAGTTTCGATCAAG+GGG + chr7_3:66546762-66546781 None:intergenic 30.0%
!!! CTTTTTTGAGTTTCGATCAA+GGG + chr7_3:66546763-66546782 None:intergenic 30.0%
!!! TCTTTTTTGAGTTTCGATCA+AGG + chr7_3:66546764-66546783 None:intergenic 30.0%
!! GTTTCGTGATTTGTTTCATA+GGG - chr7_3:66546925-66546944 Msa1118000:three_prime_UTR 30.0%
TTAGGTGTACTAATTTCTTG+TGG - chr7_3:66547119-66547138 Msa1118000:CDS 30.0%
TTGTGAACTCTGAATTAGTT+CGG - chr7_3:66547184-66547203 Msa1118000:CDS 30.0%
AAACCTGTTGCACATTAAAT+TGG + chr7_3:66547328-66547347 None:intergenic 30.0%
!! GTGGTTGTTTTATTCCATTT+TGG - chr7_3:66547353-66547372 Msa1118000:intron 30.0%
!! GTGGTTGTTTTATTCCATTT+TGG - chr7_3:66547353-66547372 Msa1118000:intron 30.0%
TGTAGTTGTTCTATTCCATT+TGG - chr7_3:66547416-66547435 Msa1118000:intron 30.0%
GTAGTTGTTCTATTCCATTT+GGG - chr7_3:66547417-66547436 Msa1118000:intron 30.0%
TGTAGTTGTTCTATTCCATT+TGG - chr7_3:66547416-66547435 Msa1118000:intron 30.0%
GTAGTTGTTCTATTCCATTT+GGG - chr7_3:66547417-66547436 Msa1118000:intron 30.0%
ATTACACGACAAATCCAAAA+TGG + chr7_3:66547559-66547578 None:intergenic 30.0%
! GTAATTGTTCGATTCCATTT+TGG - chr7_3:66547574-66547593 Msa1118000:CDS 30.0%
!!! ATTCCATTTTGGTTCAGTAA+CGG - chr7_3:66547617-66547636 Msa1118000:CDS 30.0%
!!! TTGTATGGTGCTTTTGTTTT+AGG - chr7_3:66547655-66547674 Msa1118000:intron 30.0%
!! ATCAAGATTTTTCAACTCCT+TGG + chr7_3:66547723-66547742 None:intergenic 30.0%
! ACAATTTTTGCTGACATTGA+AGG - chr7_3:66547794-66547813 Msa1118000:CDS 30.0%
TATTTAAACACACCTTTAGG+AGG + chr7_3:66547977-66547996 None:intergenic 30.0%
! AGGAATCAATCAATGTTTTC+AGG + chr7_3:66548083-66548102 None:intergenic 30.0%
CATATTCTTATCGTAAGTCT+TGG - chr7_3:66548321-66548340 Msa1118000:CDS 30.0%
! TGAATTAAGTTCTGGTTGAT+TGG - chr7_3:66548385-66548404 Msa1118000:CDS 30.0%
! TTTAAAGCTGATTCTGGAAA+CGG + chr7_3:66548475-66548494 None:intergenic 30.0%
! AGTTCATTTAAAGCTGATTC+TGG + chr7_3:66548481-66548500 None:intergenic 30.0%
!! AGAATCAGCTTTAAATGAAC+TGG - chr7_3:66548480-66548499 Msa1118000:intron 30.0%
ACTACTGCTTGAAAATTATG+AGG - chr7_3:66548510-66548529 Msa1118000:intron 30.0%
!! ATAGCTCCACTTTTGAATTT+CGG + chr7_3:66548818-66548837 None:intergenic 30.0%
AAAAAAAAAAAAGAGGGGAG+GGG - chr7_3:66549221-66549240 Msa1118000:intron 30.0%
TTGTGTACTATTGATCTTTC+TGG - chr7_3:66549278-66549297 Msa1118000:intron 30.0%
TGTGTACTATTGATCTTTCT+GGG - chr7_3:66549279-66549298 Msa1118000:intron 30.0%
ATCAACAGTAAATGACAGTA+AGG + chr7_3:66549372-66549391 None:intergenic 30.0%
! GTCATTTACTGTTGATTACT+TGG - chr7_3:66549376-66549395 Msa1118000:intron 30.0%
!!! TCACATGTTTTTTCCCCATT+AGG - chr7_3:66546729-66546748 Msa1118000:three_prime_UTR 35.0%
!!! CACATGTTTTTTCCCCATTA+GGG - chr7_3:66546730-66546749 Msa1118000:three_prime_UTR 35.0%
ATGCTATTGTGTGTACATGA+AGG - chr7_3:66546829-66546848 Msa1118000:three_prime_UTR 35.0%
!! GGTTTCGTGATTTGTTTCAT+AGG - chr7_3:66546924-66546943 Msa1118000:three_prime_UTR 35.0%
GTGACAGAACTAAACAAAGA+GGG - chr7_3:66547224-66547243 Msa1118000:CDS 35.0%
CAAGATATAACGAATCAAGC+AGG + chr7_3:66547284-66547303 None:intergenic 35.0%
TTGCCAATTTAATGTGCAAC+AGG - chr7_3:66547322-66547341 Msa1118000:intron 35.0%
ACTACACGACAAATCCAAAA+TGG + chr7_3:66547402-66547421 None:intergenic 35.0%
ACTACACGACAAATCGAAAA+TGG + chr7_3:66547496-66547515 None:intergenic 35.0%
! GTAGTTGTTCGATTCCATTT+TGG - chr7_3:66547542-66547561 Msa1118000:intron 35.0%
AAAAAGGAAGTTGCTTACCA+GGG + chr7_3:66547820-66547839 None:intergenic 35.0%
TAAAAAGGAAGTTGCTTACC+AGG + chr7_3:66547821-66547840 None:intergenic 35.0%
! TGTGGAAGGGTACATTAAAA+AGG + chr7_3:66547836-66547855 None:intergenic 35.0%
!! CATTGATTCCCATAATCAAC+AGG - chr7_3:66548426-66548445 Msa1118000:intron 35.0%
! CCTAACAACCTGTTGATTAT+GGG + chr7_3:66548437-66548456 None:intergenic 35.0%
! ACCTAACAACCTGTTGATTA+TGG + chr7_3:66548438-66548457 None:intergenic 35.0%
GTCTTACGTAAAAACTAGCA+TGG + chr7_3:66548716-66548735 None:intergenic 35.0%
! GTTTTTACGTAAGACATTCC+CGG - chr7_3:66548721-66548740 Msa1118000:intron 35.0%
!! CTTTGATTTTGCATGGATTC+CGG + chr7_3:66548795-66548814 None:intergenic 35.0%
!! ATTTCGGCTTTGATTTTGCA+TGG + chr7_3:66548802-66548821 None:intergenic 35.0%
TCAAAGCCGAAATTCAAAAG+TGG - chr7_3:66548809-66548828 Msa1118000:intron 35.0%
ATTCAGAGCAGTAGTTGATT+CGG + chr7_3:66548843-66548862 None:intergenic 35.0%
AAAAAAAAAAAGAGGGGAGG+GGG - chr7_3:66549222-66549241 Msa1118000:intron 35.0%
!! AGGGTGCTTTTGATGCAAAT+TGG - chr7_3:66546944-66546963 Msa1118000:exon 40.0%
! GGGTGCTTTTGATGCAAATT+GGG - chr7_3:66546945-66546964 Msa1118000:exon 40.0%
! TTGTTGAAGTGTCTGCAAAC+TGG - chr7_3:66546998-66547017 Msa1118000:CDS 40.0%
GGTGTACTAATTTCTTGTGG+AGG - chr7_3:66547122-66547141 Msa1118000:CDS 40.0%
!! TTTGTTTAGTTCTGTCACCG+AGG + chr7_3:66547222-66547241 None:intergenic 40.0%
GGTGACAGAACTAAACAAAG+AGG - chr7_3:66547223-66547242 Msa1118000:CDS 40.0%
! TCCATTTTGGATTTGTCGTG+TGG - chr7_3:66547366-66547385 Msa1118000:intron 40.0%
ACCACACGACAAATCCAAAA+TGG + chr7_3:66547370-66547389 None:intergenic 40.0%
TGTGGTTGTTCGATTCCATT+TGG - chr7_3:66547447-66547466 Msa1118000:intron 40.0%
GTGGTTGTTCGATTCCATTT+GGG - chr7_3:66547448-66547467 Msa1118000:intron 40.0%
ACGACACGACAAATCCAAAA+TGG + chr7_3:66547591-66547610 None:intergenic 40.0%
! GTCGTTGTTCGATTCCATTT+TGG - chr7_3:66547606-66547625 Msa1118000:CDS 40.0%
AGCATTCTGACATGGTTGTA+TGG - chr7_3:66547640-66547659 Msa1118000:intron 40.0%
! ATTAGGCGGAAGATTTTCGT+TGG + chr7_3:66547684-66547703 None:intergenic 40.0%
ATCTTCCGCCTAATGTGATA+AGG - chr7_3:66547690-66547709 Msa1118000:intron 40.0%
AGTTGCCTTATCACATTAGG+CGG + chr7_3:66547698-66547717 None:intergenic 40.0%
GCAAGTTGCCTTATCACATT+AGG + chr7_3:66547701-66547720 None:intergenic 40.0%
GCTCGTTACATAATGTGGAA+GGG + chr7_3:66547849-66547868 None:intergenic 40.0%
AGCTCGTTACATAATGTGGA+AGG + chr7_3:66547850-66547869 None:intergenic 40.0%
CTTGAGCTCGTTACATAATG+TGG + chr7_3:66547854-66547873 None:intergenic 40.0%
GCGATAGTTTCAACTTCATG+CGG + chr7_3:66547937-66547956 None:intergenic 40.0%
CACTGTTGGCAATATTTGGA+TGG + chr7_3:66548245-66548264 None:intergenic 40.0%
CATCCAAATATTGCCAACAG+TGG - chr7_3:66548243-66548262 Msa1118000:intron 40.0%
TCTCCACTGTTGGCAATATT+TGG + chr7_3:66548249-66548268 None:intergenic 40.0%
TAAGAATATGCCGTAACCCA+AGG + chr7_3:66548311-66548330 None:intergenic 40.0%
CCCATAATCAACAGGTTGTT+AGG - chr7_3:66548434-66548453 Msa1118000:intron 40.0%
!! TCAGCTTTAAATGAACTGGC+TGG - chr7_3:66548484-66548503 Msa1118000:intron 40.0%
TGTTGATCAGACAAACACCT+CGG - chr7_3:66548862-66548881 Msa1118000:CDS 40.0%
AGAAACATTAGCAGACGACT+CGG + chr7_3:66549005-66549024 None:intergenic 40.0%
AAAAAAAAAAGAGGGGAGGG+GGG - chr7_3:66549223-66549242 Msa1118000:intron 40.0%
AAGGGGACAAAACCCTAATG+GGG + chr7_3:66546745-66546764 None:intergenic 45.0%
CAAGGGGACAAAACCCTAAT+GGG + chr7_3:66546746-66546765 None:intergenic 45.0%
TCAAGGGGACAAAACCCTAA+TGG + chr7_3:66546747-66546766 None:intergenic 45.0%
ACTTACCATCAGCAGCACAA+GGG + chr7_3:66547252-66547271 None:intergenic 45.0%
TACTTACCATCAGCAGCACA+AGG + chr7_3:66547253-66547272 None:intergenic 45.0%
AACTGCACGACAAACCCAAA+TGG + chr7_3:66547465-66547484 None:intergenic 45.0%
AACTACACGACAAGCCCAAA+TGG + chr7_3:66547528-66547547 None:intergenic 45.0%
GCTCCGTTACTGAACCAAAA+TGG + chr7_3:66547623-66547642 None:intergenic 45.0%
! AGTAACGGAGCATTCTGACA+TGG - chr7_3:66547632-66547651 Msa1118000:CDS 45.0%
TGTGATAAGGCAACTTGCCA+AGG - chr7_3:66547703-66547722 Msa1118000:intron 45.0%
AACTTTAATGCCCTCAGGAG+GGG + chr7_3:66547750-66547769 None:intergenic 45.0%
CAACTTTAATGCCCTCAGGA+GGG + chr7_3:66547751-66547770 None:intergenic 45.0%
ACAACTTTAATGCCCTCAGG+AGG + chr7_3:66547752-66547771 None:intergenic 45.0%
ACGACAACTTTAATGCCCTC+AGG + chr7_3:66547755-66547774 None:intergenic 45.0%
CGGAAAACTCCACTCTCATA+AGG + chr7_3:66547917-66547936 None:intergenic 45.0%
TTTCCACACTCTCCTCCTAA+AGG - chr7_3:66547962-66547981 Msa1118000:intron 45.0%
GACACAAATCTCTCCACTGT+TGG + chr7_3:66548259-66548278 None:intergenic 45.0%
TTGTGTCAACGCGCTCAAAA+AGG - chr7_3:66548272-66548291 Msa1118000:intron 45.0%
TCAACGCGCTCAAAAAGGAT+TGG - chr7_3:66548277-66548296 Msa1118000:intron 45.0%
AAGGATTGGAATCCTAGCCT+TGG - chr7_3:66548291-66548310 Msa1118000:CDS 45.0%
! AGGATTGGAATCCTAGCCTT+GGG - chr7_3:66548292-66548311 Msa1118000:CDS 45.0%
CTCAATTACAAGCAGATGCC+GGG + chr7_3:66548742-66548761 None:intergenic 45.0%
CCTCAATTACAAGCAGATGC+CGG + chr7_3:66548743-66548762 None:intergenic 45.0%
CGGCATCTGCTTGTAATTGA+GGG - chr7_3:66548741-66548760 Msa1118000:intron 45.0%
GGCATCTGCTTGTAATTGAG+GGG - chr7_3:66548742-66548761 Msa1118000:intron 45.0%
AAGAGGAACTAGTCAGGATC+AGG - chr7_3:66548961-66548980 Msa1118000:intron 45.0%
TAATGTTTCTTCTGCTGCCG+CGG - chr7_3:66549015-66549034 Msa1118000:intron 45.0%
TGCTCATGCACCACAAAAGA+GGG - chr7_3:66549048-66549067 Msa1118000:intron 45.0%
! GTTCCAATGTCCCTCTTTTG+TGG + chr7_3:66549061-66549080 None:intergenic 45.0%
CATTGGAACTGCTAAAGCTC+AGG - chr7_3:66549072-66549091 Msa1118000:intron 45.0%
!! ACAAATAATAATAAAAATAA+GGG + chr7_3:66549317-66549336 None:intergenic 5.0%
!! AACAAATAATAATAAAAATA+AGG + chr7_3:66549318-66549337 None:intergenic 5.0%
TGTGCAACAGGTTTGTCGTG+TGG - chr7_3:66547334-66547353 Msa1118000:intron 50.0%
TTCCATTTGGGCTTGTCGTG+TGG - chr7_3:66547429-66547448 Msa1118000:intron 50.0%
AACCACACGACAAGCCCAAA+TGG + chr7_3:66547434-66547453 None:intergenic 50.0%
! AGGCGGAAGATTTTCGTTGG+TGG + chr7_3:66547681-66547700 None:intergenic 50.0%
TCTTGATGAAACCCCTCCTG+AGG - chr7_3:66547736-66547755 Msa1118000:intron 50.0%
CTTGATGAAACCCCTCCTGA+GGG - chr7_3:66547737-66547756 Msa1118000:intron 50.0%
TTTGCTGACATTGAAGGCCC+TGG - chr7_3:66547800-66547819 Msa1118000:CDS 50.0%
GCTGCAACTCCTTATGAGAG+TGG - chr7_3:66547905-66547924 Msa1118000:intron 50.0%
! ACACCTTTAGGAGGAGAGTG+TGG + chr7_3:66547968-66547987 None:intergenic 50.0%
!! GGAATCCTAGCCTTGGGTTA+CGG - chr7_3:66548298-66548317 Msa1118000:CDS 50.0%
ATATGCCGTAACCCAAGGCT+AGG + chr7_3:66548306-66548325 None:intergenic 50.0%
! AGGAGTATGCTAGACATGCC+AGG - chr7_3:66548530-66548549 Msa1118000:intron 50.0%
TGCCAGGTGAGTTAAGCCAA+TGG - chr7_3:66548546-66548565 Msa1118000:intron 50.0%
!! CACCATTGGCTTAACTCACC+TGG + chr7_3:66548551-66548570 None:intergenic 50.0%
CCGGCATCTGCTTGTAATTG+AGG - chr7_3:66548740-66548759 Msa1118000:intron 50.0%
! GGGCTTTAATTGCGACATGC+AGG - chr7_3:66548762-66548781 Msa1118000:intron 50.0%
! GCGACATGCAGGCTTTTAAC+CGG - chr7_3:66548773-66548792 Msa1118000:intron 50.0%
! GATGTCAGCATTAAGCACCG+AGG + chr7_3:66548882-66548901 None:intergenic 50.0%
GACATCAAAACCATGCCGTC+AGG - chr7_3:66548896-66548915 Msa1118000:intron 50.0%
ACTAGTTCCTCTTGCTCCAG+TGG + chr7_3:66548954-66548973 None:intergenic 50.0%
TGGAGCAAGAGGAACTAGTC+AGG - chr7_3:66548955-66548974 Msa1118000:intron 50.0%
TAGTCAGGATCAGGCAGTAG+TGG - chr7_3:66548970-66548989 Msa1118000:intron 50.0%
AGTCAGGATCAGGCAGTAGT+GGG - chr7_3:66548971-66548990 Msa1118000:intron 50.0%
ATGAGCAGCAGAAACTACCG+CGG + chr7_3:66549035-66549054 None:intergenic 50.0%
CTGCTCATGCACCACAAAAG+AGG - chr7_3:66549047-66549066 Msa1118000:intron 50.0%
GCACCACAAAAGAGGGACAT+TGG - chr7_3:66549055-66549074 Msa1118000:intron 50.0%
GGGGAAGGGATATGGGTAAT+GGG - chr7_3:66549242-66549261 Msa1118000:intron 50.0%
TTCGGAAGCTCCTCACTCCT+CGG - chr7_3:66547202-66547221 Msa1118000:CDS 55.0%
AGTTCTGTCACCGAGGAGTG+AGG + chr7_3:66547215-66547234 None:intergenic 55.0%
ATTGGCAGGAGCAGCACCAT+TGG + chr7_3:66548565-66548584 None:intergenic 55.0%
GTTGAGGGCAATGACAGTGG+TGG + chr7_3:66548932-66548951 None:intergenic 55.0%
GTGGTTGAGGGCAATGACAG+TGG + chr7_3:66548935-66548954 None:intergenic 55.0%
CTGTCATTGCCCTCAACCAC+TGG - chr7_3:66548935-66548954 Msa1118000:intron 55.0%
CCTCTTGCTCCAGTGGTTGA+GGG + chr7_3:66548947-66548966 None:intergenic 55.0%
TCCTCTTGCTCCAGTGGTTG+AGG + chr7_3:66548948-66548967 None:intergenic 55.0%
AAAAAAGAGGGGAGGGGGGA+AGG - chr7_3:66549227-66549246 Msa1118000:intron 55.0%
AAAAAGAGGGGAGGGGGGAA+GGG - chr7_3:66549228-66549247 Msa1118000:intron 55.0%
GGGGGAAGGGATATGGGTAA+TGG - chr7_3:66549241-66549260 Msa1118000:intron 55.0%
! GGGTTCCCTTGTGCTGCTGA+TGG - chr7_3:66547244-66547263 Msa1118000:CDS 60.0%
CCCTCAACCACTGGAGCAAG+AGG - chr7_3:66548944-66548963 Msa1118000:intron 60.0%
CAGAGCAGCACCTGACGGCA+TGG + chr7_3:66548909-66548928 None:intergenic 65.0%
GGTGGCAGAGCAGCACCTGA+CGG + chr7_3:66548914-66548933 None:intergenic 65.0%
AGGGGAGGGGGGAAGGGATA+TGG - chr7_3:66549234-66549253 Msa1118000:intron 65.0%
GGGGAGGGGGGAAGGGATAT+GGG - chr7_3:66549235-66549254 Msa1118000:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 66546677 66549429 66546677 ID=Msa1118000;Name=Msa1118000
chr7_3 mRNA 66546677 66549429 66546677 ID=Msa1118000-mRNA-1;Parent=Msa1118000;Name=Msa1118000-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.83|0.71|0.85|0.83|0.71|7|285|286
chr7_3 exon 66546677 66547322 66546677 ID=Msa1118000-mRNA-1:exon:16156;Parent=Msa1118000-mRNA-1
chr7_3 exon 66547555 66547658 66547555 ID=Msa1118000-mRNA-1:exon:16155;Parent=Msa1118000-mRNA-1
chr7_3 exon 66547774 66547886 66547774 ID=Msa1118000-mRNA-1:exon:16154;Parent=Msa1118000-mRNA-1
chr7_3 exon 66548123 66548200 66548123 ID=Msa1118000-mRNA-1:exon:16153;Parent=Msa1118000-mRNA-1
chr7_3 exon 66548285 66548429 66548285 ID=Msa1118000-mRNA-1:exon:16152;Parent=Msa1118000-mRNA-1
chr7_3 exon 66548841 66548896 66548841 ID=Msa1118000-mRNA-1:exon:16151;Parent=Msa1118000-mRNA-1
chr7_3 exon 66549426 66549429 66549426 ID=Msa1118000-mRNA-1:exon:16150;Parent=Msa1118000-mRNA-1
chr7_3 CDS 66549426 66549429 66549426 ID=Msa1118000-mRNA-1:cds;Parent=Msa1118000-mRNA-1
chr7_3 CDS 66548841 66548896 66548841 ID=Msa1118000-mRNA-1:cds;Parent=Msa1118000-mRNA-1
chr7_3 CDS 66548285 66548429 66548285 ID=Msa1118000-mRNA-1:cds;Parent=Msa1118000-mRNA-1
chr7_3 CDS 66548123 66548200 66548123 ID=Msa1118000-mRNA-1:cds;Parent=Msa1118000-mRNA-1
chr7_3 CDS 66547774 66547886 66547774 ID=Msa1118000-mRNA-1:cds;Parent=Msa1118000-mRNA-1
chr7_3 CDS 66547555 66547658 66547555 ID=Msa1118000-mRNA-1:cds;Parent=Msa1118000-mRNA-1
chr7_3 CDS 66546962 66547322 66546962 ID=Msa1118000-mRNA-1:cds;Parent=Msa1118000-mRNA-1
chr7_3 three_prime_UTR 66546677 66546961 66546677 ID=Msa1118000-mRNA-1:three_prime_utr;Parent=Msa1118000-mRNA-1
Gene Sequence

>Msa1118000

ATGGCTCCTCACTCCTCGGTGACAGAACTAAACAAAGAGGGTTCCCTTGTGCTGCTGATGGCCACCAACGAAAATCTTCCGCCTAATGTGATAAGGCAACTTGCCAAGGAGTTGAAAAATCTTGATGAAACCCCTCCTGAGGGCATTAAAGTTGTCGTGAATGATGATGATTTTTCAACAATTTTTGCTGACATTGAAGGCCCTGCTGCAACTCCTTATGAGAGTGGAGTTTTCCGCATGAAGTTGAAACTATCGCATGATTTTCCACACTCTCCTCCTAAAGGATATTTCTTGACCAAGATTTTCCATCCAAATATTGCCAACAGTGGAGAGATTTGTGTCAACGCGCTCAAAAAGGATTGGAATCCTAGCCTTGGGTTACGGCATATTCTTATCGTTGTTAGGTGTCTATTGATTGAACCGTTTCCAGAATCAGCTTTAAATGAACTGGCTGGTAAACTACTGCTTGAAAATTATGAGGAGTATGCTAGACATGCCAGGCTTTTAACCGGAATCCATGCAAAATCAAAGCCGAAATTCAAAAGTGGAGCTATAACCGAATCAACTACTGCTCTGAATGTTGATCAGACAAACACCTCGGTGCTTAATGCTGACATCAAAACCATGCCGTCAGGTGCTGCTCTGCCACCACTGTCATTGCCCTCAACCACTGGAGCAAGAGGAACTAGTCAGGATCAGGCAGTAGTGGGCGTTCTGACCGAGTCGTCTGCTAATGTTTCTTCTGCTGCCGCGGTAGTTTCTGCTGCTCATGCACCACAAAAGAGGGACATTGGAACTGCTAAAGCTCAGGCAGACAAGAAGAAGCTAGATGCTAGAAAGAAAAGTTTGAAAAGATTGTGA
Protein sequence

>Msa1118000

MAPHSSVTELNKEGSLVLLMATNENLPPNVIRQLAKELKNLDETPPEGIKVVVNDDDFSTIFADIEGPAATPYESGVFRMKLKLSHDFPHSPPKGYFLTKIFHPNIANSGEICVNALKKDWNPSLGLRHILIVVRCLLIEPFPESALNELAGKLLLENYEEYARHARLLTGIHAKSKPKFKSGAITESTTALNVDQTNTSVLNADIKTMPSGAALPPLSLPSTTGARGTSQDQAVVGVLTESSANVSSAAAVVSAAHAPQKRDIGTAKAQADKKKLDARKKSLKRL*