AlfalfaGEDB | Msa1125740

M. sativa cultivar zhongmu-4 / Msa1125740

KEGG: K14488
GO: -
PFAM: PF02519.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1125740	XP_003625848.1	95.385	130	6	0	1	130	1	130	1.84e-85	255

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1125740	sp\|O65695\|SAU50_ARATH	45.902	61	33	0	53	113	46	106	9.45e-15	68.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1125740	G7KQX4	95.385	130	6	0	1	130	1	130	8.80e-86	255

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0121300	Msa1125740	0.804632	1.967722e-49	-8.615850e-47
Msa0166190	Msa1125740	0.811951	5.414027e-51	-8.615850e-47
Msa0287480	Msa1125740	0.801869	7.343898e-49	-8.615850e-47
Msa0709520	Msa1125740	0.974303	6.414843e-138	-8.615850e-47
Msa0790870	Msa1125740	0.967569	1.852958e-127	-8.615850e-47
Msa1084450	Msa1125740	0.864367	1.279704e-64	-8.615850e-47
Msa1125740	Msa1171380	0.973753	5.759597e-137	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1125740	MtrunA17_Chr7g0266581	95.385	130	6	0	1	130	1	130	1.69e-89	255
Msa1125740	MtrunA17_Chr7g0266571	90.769	130	12	0	1	130	1	130	8.47e-69	203
Msa1125740	MtrunA17_Chr1g0178451	65.185	135	36	3	1	130	1	129	1.69e-47	149
Msa1125740	MtrunA17_Chr2g0301531	54.839	62	28	0	53	114	79	140	3.86e-20	80.9
Msa1125740	MtrunA17_Chr4g0070071	42.353	85	49	0	37	121	56	140	2.06e-19	78.6
Msa1125740	MtrunA17_Chr3g0145371	45.902	61	33	0	53	113	48	108	8.93e-16	68.2
Msa1125740	MtrunA17_Chr7g0214201	43.056	72	37	1	53	120	70	141	1.12e-15	69.3
Msa1125740	MtrunA17_Chr4g0036161	45.902	61	33	0	53	113	39	99	2.16e-15	66.6
Msa1125740	MtrunA17_Chr4g0036841	40.260	77	45	1	38	113	23	99	3.02e-15	66.6
Msa1125740	MtrunA17_Chr1g0147571	44.776	67	37	0	48	114	102	168	3.11e-15	68.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1125740	AT2G36210.1	45.185	135	69	2	1	130	1	135	1.57e-32	112
Msa1125740	AT5G20810.1	51.562	64	31	0	53	116	82	145	3.01e-18	76.3
Msa1125740	AT5G20810.2	51.562	64	31	0	53	116	82	145	6.07e-18	75.9
Msa1125740	AT3G51200.1	43.421	76	35	1	38	113	36	103	1.05e-17	73.2
Msa1125740	AT5G66260.1	45.902	61	33	0	53	113	37	97	4.94e-17	71.2
Msa1125740	AT3G43120.1	46.479	71	37	1	53	123	82	151	1.05e-16	72.0
Msa1125740	AT4G34760.1	45.902	61	33	0	53	113	46	106	9.61e-16	68.2
Msa1125740	AT4G34780.1	40.964	83	45	2	38	116	16	98	1.11e-15	68.2
Msa1125740	AT2G21220.1	47.541	61	32	0	53	113	43	103	1.17e-15	67.8
Msa1125740	AT3G20220.1	42.029	69	38	2	53	119	50	118	9.10e-15	65.9

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Find 23 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTGTTGAAGGAATATA+AGG	0.315149	7_3:-77221784	Msa1125740:CDS
AACAATTGTAAGTTTGACAT+GGG	0.582976	7_3:-77221525	Msa1125740:CDS
AGAAAGCATACAATGAATTT+GGG	0.366303	7_3:-77221623	Msa1125740:CDS
AGAGATACGTGGTTCCAACC+CGG	0.610242	7_3:-77221680	Msa1125740:CDS
ATAATGGATGAGAGAGGTAC+CGG	0.538476	7_3:+77221661	None:intergenic
ATGAATTTGGGTTTGAACAG+AGG	0.563400	7_3:-77221611	Msa1125740:CDS
GAGAAAGCATACAATGAATT+TGG	0.161291	7_3:-77221624	Msa1125740:CDS
GGAGGAACGACAGAGATACG+TGG	0.777826	7_3:-77221691	Msa1125740:CDS
GGATGAGAGAGGTACCGGGT+TGG	0.623880	7_3:+77221666	None:intergenic
GTTACAATTAACAAGATGTT+AGG	0.272598	7_3:-77221885	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATCAAGAAACTTGCAAAAA+AGG	-	chr7_3:77221523-77221542	Msa1125740:CDS	30.0%
GAAACTTGCAAAAAAGGTAA+AGG	-	chr7_3:77221529-77221548	Msa1125740:CDS	30.0%
ATTCCAACCACAAAAAAAGT+TGG	-	chr7_3:77221620-77221639	Msa1125740:CDS	30.0%
TTCCAACCACAAAAAAAGTT+GGG	-	chr7_3:77221621-77221640	Msa1125740:CDS	30.0%
TCCATTATTCAAGATGTTGT+TGG	-	chr7_3:77221724-77221743	Msa1125740:CDS	30.0%
TCCAACAACATCTTGAATAA+TGG	+	chr7_3:77221728-77221747	None:intergenic	30.0%
GCAAAAAAGGTAAAGGTTGT+AGG	-	chr7_3:77221536-77221555	Msa1125740:CDS	35.0%
TCAACAACTTTAAGTGAGCA+GGG	+	chr7_3:77221577-77221596	None:intergenic	35.0%
TTCAACAACTTTAAGTGAGC+AGG	+	chr7_3:77221578-77221597	None:intergenic	35.0%
TGCTCACTTAAAGTTGTTGA+AGG	-	chr7_3:77221577-77221596	Msa1125740:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	77221500	77221892	77221500	ID=Msa1125740;Name=Msa1125740
chr7_3	mRNA	77221500	77221892	77221500	ID=Msa1125740-mRNA-1;Parent=Msa1125740;Name=Msa1125740-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|130
chr7_3	exon	77221500	77221892	77221500	ID=Msa1125740-mRNA-1:exon:21270;Parent=Msa1125740-mRNA-1
chr7_3	CDS	77221500	77221892	77221500	ID=Msa1125740-mRNA-1:cds;Parent=Msa1125740-mRNA-1

Gene Sequence

>Msa1125740

ATGTTAGGGAAAAAGATTGTATCGATCAAGAAACTTGCAAAAAAGGTAAAGGTTGTAGGTAGAGTTGACTCTGACCCTGCTCACTTAAAGTTGTTGAAGGAATATAAGGAAGAAGAAAAAATTCCAACCACAAAAAAAGTTGGGTCCAAAAAAAGTGGTGTTTTTGCACTATATGTTGGGGAGGAACGACAGAGATACGTGGTTCCAACCCGGTACCTCTCTCATCCATTATTCAAGATGTTGTTGGAGAAAGCATACAATGAATTTGGGTTTGAACAGAGGAATGGCTTGGTAGTTCCATGTAGTGTTTCAGCATTTCATGAAGTGGTTAATGCTATAGAATGTAACAATTGTAAGTTTGACATGGGAAATATTTTTGATGAATTGATTTGA

Protein sequence

>Msa1125740

MLGKKIVSIKKLAKKVKVVGRVDSDPAHLKLLKEYKEEEKIPTTKKVGSKKSGVFALYVGEERQRYVVPTRYLSHPLFKMLLEKAYNEFGFEQRNGLVVPCSVSAFHEVVNAIECNNCKFDMGNIFDELI*