AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1134530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1134530 MtrunA17_Chr7g0214791 95.735 211 7 1 1 211 1 209 8.43e-143 404
Msa1134530 MtrunA17_Chr7g0214791 100.000 145 0 0 109 253 207 351 4.30e-99 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1134530 AT1G44960.1 67.299 211 67 1 32 242 26 234 2.47e-103 301

Find 70 sgRNAs with CRISPR-Local

Find 285 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTCCGGTACTGAAATT+TGG 0.132573 7_4:+1045909 None:intergenic
TATCTGATAAACTAGGAATT+TGG 0.145494 7_4:-1050878 Msa1134530:CDS
GCTCTAAGAAATCTCAATTT+TGG 0.229017 7_4:-1046003 Msa1134530:CDS
AGTTCTTCAGGTTAGTATTC+AGG 0.245543 7_4:-1046291 Msa1134530:intron
ACTGGTGCTTCTATTCTCTT+TGG 0.281656 7_4:-1050783 Msa1134530:CDS
ACTAGATAAAATACCAACTA+TGG 0.282343 7_4:+1045967 None:intergenic
AATACTAACAACAACAAAAT+TGG 0.287741 7_4:+1045702 None:intergenic
ATCTGATAAACTAGGAATTT+GGG 0.313710 7_4:-1050877 Msa1134530:CDS
GACTTGATCTTCATTTAATC+TGG 0.331073 7_4:-1045630 Msa1134530:three_prime_UTR
AATTTCCGGTACTGAAATTT+GGG 0.342524 7_4:+1045910 None:intergenic
CCTTAGCTGCTACTGAAGTT+AGG 0.357035 7_4:-1046132 Msa1134530:CDS
GCAGCTGTTGCTACAGCCTC+TGG 0.372165 7_4:-1046025 Msa1134530:CDS
ATAAAGAATCATTGATGAAA+TGG 0.374496 7_4:-1050911 Msa1134530:CDS
TACCGATCGAACTGTCTGTT+TGG 0.377737 7_4:-1045760 Msa1134530:three_prime_UTR
TCTTTGTCAGAAGAAATTTC+CGG 0.385161 7_4:+1045896 None:intergenic
CAGCAATGGACTGGGCTAGA+AGG 0.407462 7_4:-1046258 Msa1134530:CDS
ACCGATCGAACTGTCTGTTT+GGG 0.409473 7_4:-1045759 Msa1134530:three_prime_UTR
GAATAGAAGCACCAGTTTCA+AGG 0.425167 7_4:+1050790 None:intergenic
CCTTATCTTTCTCCATTGGC+AGG 0.426737 7_4:-1050710 Msa1134530:intron
TTTCTTCTTCCTACCATTGT+CGG 0.428961 7_4:-1046097 Msa1134530:CDS
GGAAGTTTGTTCTTCTTGCT+CGG 0.432228 7_4:-1046189 Msa1134530:CDS
GTGCTTCTATTCTCTTTGGT+TGG 0.434613 7_4:-1050779 Msa1134530:CDS
GGCGGAGAAGCAATCGGAAT+CGG 0.440129 7_4:-1050945 Msa1134530:CDS
ACATCTATAGGAAGTCTTGC+TGG 0.441068 7_4:-1046049 Msa1134530:CDS
TAAGGTATAGTTAATGATAT+AGG 0.443398 7_4:+1046150 None:intergenic
ACATAATGGCGGGGTGGTGG+TGG 0.456256 7_4:-1051016 Msa1134530:exon
GAAGCAATCGGAATCGGAAT+CGG 0.456425 7_4:-1050939 Msa1134530:CDS
GCCTTACTACTCCGATCATA+TGG 0.458022 7_4:-1050966 Msa1134530:CDS
GCTTCCTTATCTTTCTCCAT+TGG 0.464011 7_4:-1050714 Msa1134530:CDS
ATCTTACAGAATACATCTAT+AGG 0.469430 7_4:-1046061 Msa1134530:CDS
AAAATCAATAACATAATGGC+GGG 0.471689 7_4:-1051026 Msa1134530:exon
AGATGTATTCTGTAAGATCA+TGG 0.472448 7_4:+1046066 None:intergenic
TTCGTCCCAAATTTCAGTAC+CGG 0.474329 7_4:-1045915 Msa1134530:CDS
ATAACATAATGGCGGGGTGG+TGG 0.489266 7_4:-1051019 Msa1134530:exon
ATTCAACCTCAGCAATGGAC+TGG 0.500375 7_4:-1046267 Msa1134530:CDS
TCATATGGCGGAGAAGCAAT+CGG 0.500801 7_4:-1050951 Msa1134530:CDS
TTCAACCTCAGCAATGGACT+GGG 0.502524 7_4:-1046266 Msa1134530:CDS
GCCATATGATCGGAGTAGTA+AGG 0.504927 7_4:+1050965 None:intergenic
CCTAACTTCAGTAGCAGCTA+AGG 0.506076 7_4:+1046132 None:intergenic
GATGTATTCTGTAAGATCAT+GGG 0.518629 7_4:+1046067 None:intergenic
CTAGATAAAATACCAACTAT+GGG 0.519361 7_4:+1045968 None:intergenic
CCTGCCAATGGAGAAAGATA+AGG 0.520262 7_4:+1050710 None:intergenic
TTGCTTCTCCGCCATATGAT+CGG 0.526914 7_4:+1050955 None:intergenic
CTCGCTCAACTCCATTTGCA+AGG 0.541452 7_4:+1046218 None:intergenic
AAAATTATCTAGTAGTGAAG+AGG 0.553803 7_4:-1045844 Msa1134530:three_prime_UTR
AATCATATGTTACCTGCCAA+TGG 0.556218 7_4:+1050698 None:intergenic
AGAGATTTATCTGATAAACT+AGG 0.558993 7_4:-1050885 Msa1134530:CDS
GTGTGAATAGCGATGTAAAG+TGG 0.559311 7_4:+1050849 None:intergenic
GGGATGCATCCGACAATGGT+AGG 0.579071 7_4:+1046088 None:intergenic
TTGAGATTTCTTAGAGCCAG+AGG 0.582555 7_4:+1046009 None:intergenic
TCTAGCCCAGTCCATTGCTG+AGG 0.592500 7_4:+1046261 None:intergenic
ATGGAGTTGAGCGAGATGGT+TGG 0.593190 7_4:-1046210 Msa1134530:CDS
CAGGAATTCAACCTCAGCAA+TGG 0.593899 7_4:-1046272 Msa1134530:CDS
TCCCAAACAGACAGTTCGAT+CGG 0.605038 7_4:+1045758 None:intergenic
CATGGGGATGCATCCGACAA+TGG 0.608315 7_4:+1046084 None:intergenic
ATGTATTCTGTAAGATCATG+GGG 0.610828 7_4:+1046068 None:intergenic
TCTGTTAGTAAACTGGTGCG+TGG 0.633463 7_4:-1045810 Msa1134530:three_prime_UTR
GTATAGTTAATGATATAGGA+TGG 0.638706 7_4:+1046154 None:intergenic
GTTTCAAGGAAAATAGCAGA+AGG 0.643085 7_4:+1050804 None:intergenic
GCAAATGGAGTTGAGCGAGA+TGG 0.646060 7_4:-1046214 Msa1134530:CDS
AAATCAATAACATAATGGCG+GGG 0.646436 7_4:-1051025 Msa1134530:exon
CAGTTCGATCGGTAAACTGA+AGG 0.647256 7_4:+1045769 None:intergenic
TTAATGATATAGGATGGCAC+AGG 0.650154 7_4:+1046160 None:intergenic
GGGGTGGTGGTGGAAAACAG+CGG 0.679901 7_4:-1051006 Msa1134530:CDS
TAGATAAAATACCAACTATG+GGG 0.680141 7_4:+1045969 None:intergenic
TCAATAACATAATGGCGGGG+TGG 0.680877 7_4:-1051022 Msa1134530:exon
TCGATCGGTAAACTGAAGGA+CGG 0.705675 7_4:+1045773 None:intergenic
TTACTACTCCGATCATATGG+CGG 0.710739 7_4:-1050963 Msa1134530:CDS
ATATGATCGGAGTAGTAAGG+CGG 0.729764 7_4:+1050968 None:intergenic
AGAAAGATAAGGAAGCAGCG+AGG 0.748647 7_4:+1050721 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAGTTGATTTAATAATTA+AGG + chr7_4:1047517-1047536 None:intergenic 10.0%
!!! TTAATTTATTATGTTGTTTT+AGG - chr7_4:1047535-1047554 Msa1134530:intron 10.0%
!! TTAATTCATTAAATTGTTAT+AGG - chr7_4:1048204-1048223 Msa1134530:intron 10.0%
!! TAGTCATTAATATTAATAAT+AGG + chr7_4:1048637-1048656 None:intergenic 10.0%
!! AAATAAACATGTTTAAATTT+AGG - chr7_4:1049469-1049488 Msa1134530:intron 10.0%
!!! AATTTTTCAACAAAATTTTA+AGG + chr7_4:1049569-1049588 None:intergenic 10.0%
!! TAGAAACTTAGAATTAAAAA+AGG - chr7_4:1046258-1046277 Msa1134530:CDS 15.0%
!! AGAAACTTAGAATTAAAAAA+GGG - chr7_4:1046259-1046278 Msa1134530:CDS 15.0%
!!! TAGAATTAGTTGAATAATTT+TGG - chr7_4:1046469-1046488 Msa1134530:intron 15.0%
!! TATATTATTAAATGTGTGTT+TGG - chr7_4:1046886-1046905 Msa1134530:intron 15.0%
!!! TGATTTTGTAAATTTGATTT+TGG - chr7_4:1046941-1046960 Msa1134530:intron 15.0%
!!! GAATAAATTTGAGTTAAATA+TGG - chr7_4:1047014-1047033 Msa1134530:intron 15.0%
!!! AATTTTTGTTTTTTCAAAAG+TGG - chr7_4:1047153-1047172 Msa1134530:intron 15.0%
!! TTAAATAAACTAATTGTGTT+TGG - chr7_4:1047621-1047640 Msa1134530:intron 15.0%
!!! TTTTGTAGTTTTTTAAATTG+TGG - chr7_4:1047785-1047804 Msa1134530:intron 15.0%
!! AAAAAAATACAAAGAATTTG+AGG - chr7_4:1048347-1048366 Msa1134530:intron 15.0%
!! CTGAAAAATCAATAACATAA+TGG - chr7_4:1045725-1045744 Msa1134530:three_prime_UTR 20.0%
!! AAAAATCAATAACATAATGG+CGG - chr7_4:1045728-1045747 Msa1134530:three_prime_UTR 20.0%
!! ATAAAGAATCATTGATGAAA+TGG - chr7_4:1045844-1045863 Msa1134530:three_prime_UTR 20.0%
!!! TTTCTTTTTCACATTTTCAT+CGG + chr7_4:1046164-1046183 None:intergenic 20.0%
!!! TAGTTGAATAATTTTGGTTA+AGG - chr7_4:1046475-1046494 Msa1134530:intron 20.0%
!!! TTGTTTTCAATTAAGTTTGT+AGG - chr7_4:1046662-1046681 Msa1134530:intron 20.0%
!!! TGTTTTCAATTAAGTTTGTA+GGG - chr7_4:1046663-1046682 Msa1134530:intron 20.0%
!!! GGTTTAAGTTTAATCAATTA+TGG - chr7_4:1047065-1047084 Msa1134530:intron 20.0%
!! TTAAGTTTAATCAATTATGG+AGG - chr7_4:1047068-1047087 Msa1134530:intron 20.0%
!!! AAAACAAAAATTGATTCTAG+AGG + chr7_4:1047145-1047164 None:intergenic 20.0%
!! TAATTGGATGTAAATTAGTT+TGG - chr7_4:1047359-1047378 Msa1134530:intron 20.0%
!! ATGATATGATGAATTCTAAT+TGG - chr7_4:1047407-1047426 Msa1134530:intron 20.0%
!! AAATTAACAACTAAAATGCT+AGG + chr7_4:1047913-1047932 None:intergenic 20.0%
!! AAACTATCAAATAAAACTCT+TGG + chr7_4:1048166-1048185 None:intergenic 20.0%
!! TTAAATTGTTATAGGAAGTT+AGG - chr7_4:1048212-1048231 Msa1134530:intron 20.0%
!! CTTAATAAACTATTTGTGTT+TGG - chr7_4:1048296-1048315 Msa1134530:intron 20.0%
!! CATACATGTTTATATCATTA+AGG - chr7_4:1048781-1048800 Msa1134530:intron 20.0%
!! AATCTTTGTAATCAAATGAT+TGG - chr7_4:1048971-1048990 Msa1134530:intron 20.0%
!!! TTGTTTTTTTTTGTTATGCT+AGG - chr7_4:1049185-1049204 Msa1134530:intron 20.0%
!!! AAATATTCTATGGTAATGAA+GGG - chr7_4:1049229-1049248 Msa1134530:intron 20.0%
!!! GCTTATTTTTGTTTTGTATA+CGG - chr7_4:1049273-1049292 Msa1134530:intron 20.0%
!! TGAGTTAAAATTGATAGAAT+AGG - chr7_4:1049377-1049396 Msa1134530:intron 20.0%
!! TGTTGAAAAATTTGATAAGT+TGG - chr7_4:1049577-1049596 Msa1134530:intron 20.0%
!!! CTTCTAAAATAAAAAATCAC+AGG + chr7_4:1049684-1049703 None:intergenic 20.0%
!! TTTATAAAAATGTATCCTCT+TGG - chr7_4:1049776-1049795 Msa1134530:intron 20.0%
!! AATTATAAGTGGTAGTTTAT+AGG - chr7_4:1050062-1050081 Msa1134530:intron 20.0%
!! ATTTGAGAAAATGGATAAAT+TGG + chr7_4:1050288-1050307 None:intergenic 20.0%
!! GTGATAAATATTTGAGAAAA+TGG + chr7_4:1050297-1050316 None:intergenic 20.0%
!! TTTATTAGTAACAACTAACA+AGG - chr7_4:1050343-1050362 Msa1134530:intron 20.0%
!! GACAATACATTATTAACATA+TGG + chr7_4:1050373-1050392 None:intergenic 20.0%
!!! TATTTTAAAATCCTTGCAAA+TGG - chr7_4:1050526-1050545 Msa1134530:intron 20.0%
!! TAAGGTATAGTTAATGATAT+AGG + chr7_4:1050608-1050627 None:intergenic 20.0%
!! AATACTAACAACAACAAAAT+TGG + chr7_4:1051056-1051075 None:intergenic 20.0%
!! AAGAGTATAGTCAACAAAAA+CGG - chr7_4:1045649-1045668 Msa1134530:three_prime_UTR 25.0%
! AAAATCAATAACATAATGGC+GGG - chr7_4:1045729-1045748 Msa1134530:three_prime_UTR 25.0%
! AGAGATTTATCTGATAAACT+AGG - chr7_4:1045870-1045889 Msa1134530:CDS 25.0%
! TATCTGATAAACTAGGAATT+TGG - chr7_4:1045877-1045896 Msa1134530:CDS 25.0%
! ATCTGATAAACTAGGAATTT+GGG - chr7_4:1045878-1045897 Msa1134530:CDS 25.0%
!!! GTTTTTGGTAAAAAAAGTAG+CGG - chr7_4:1046510-1046529 Msa1134530:intron 25.0%
! ACTGATAAGATAGTTTATAG+TGG - chr7_4:1046536-1046555 Msa1134530:intron 25.0%
! ACTTATAAGCTAACTTATAG+CGG - chr7_4:1046562-1046581 Msa1134530:intron 25.0%
!! GTTTTCAATTAAGTTTGTAG+GGG - chr7_4:1046664-1046683 Msa1134530:intron 25.0%
!! TTATTTTCATGAGCTTATAG+AGG + chr7_4:1046746-1046765 None:intergenic 25.0%
! AAGACATAAAATTGACCTTA+TGG + chr7_4:1046844-1046863 None:intergenic 25.0%
! CGATAAATTTAGTGTATGTT+TGG + chr7_4:1047181-1047200 None:intergenic 25.0%
!!! ATTTTTTGTCGATGAGAAAA+AGG - chr7_4:1047237-1047256 Msa1134530:intron 25.0%
!!! TGTAGTTATTTGTTATGTTG+TGG - chr7_4:1047300-1047319 Msa1134530:intron 25.0%
! AAATTGACAACTAAAACACT+AGG + chr7_4:1047493-1047512 None:intergenic 25.0%
!!! TTATGTTGTTTTAGGAAGTA+AGG - chr7_4:1047543-1047562 Msa1134530:intron 25.0%
! GTTTATTAAGTTAAAGAGCT+TGG + chr7_4:1048286-1048305 None:intergenic 25.0%
! AAAATACAAAGAATTTGAGG+TGG - chr7_4:1048350-1048369 Msa1134530:intron 25.0%
! TGTACTATTATGTAATGCTT+GGG - chr7_4:1048507-1048526 Msa1134530:intron 25.0%
!!! TCTTTTGTATGTCGATTTAT+AGG - chr7_4:1048817-1048836 Msa1134530:intron 25.0%
!! AAATTTTGTGGTTTCTTTGT+TGG - chr7_4:1048847-1048866 Msa1134530:intron 25.0%
! TGAAGCTTATATTAAAAGCA+GGG - chr7_4:1048879-1048898 Msa1134530:intron 25.0%
! TCATTTGATTACAAAGATTG+AGG + chr7_4:1048970-1048989 None:intergenic 25.0%
! TTTGTAATCAAATGATTGGT+TGG - chr7_4:1048975-1048994 Msa1134530:intron 25.0%
!! TAATTTGAGATGAAAGTTAC+TGG - chr7_4:1049000-1049019 Msa1134530:intron 25.0%
! TAGAATATTTGCACAGAAAA+AGG + chr7_4:1049219-1049238 None:intergenic 25.0%
!! TTTTCTGTGCAAATATTCTA+TGG - chr7_4:1049219-1049238 Msa1134530:intron 25.0%
!! CAAATATTCTATGGTAATGA+AGG - chr7_4:1049228-1049247 Msa1134530:intron 25.0%
!! AGTTTTGCATCTTCATTTTA+CGG - chr7_4:1049409-1049428 Msa1134530:intron 25.0%
!!! AAATTTGATAAGTTGGTTGA+AGG - chr7_4:1049584-1049603 Msa1134530:intron 25.0%
! CTATACCGAATTATATCATT+GGG - chr7_4:1049873-1049892 Msa1134530:intron 25.0%
! ATATATTGTCTGATTCAAGT+TGG - chr7_4:1049909-1049928 Msa1134530:intron 25.0%
! AAAGAAGCAATAACTTAAAC+TGG + chr7_4:1050002-1050021 None:intergenic 25.0%
! GTCATTGTATGAATTATAAG+TGG - chr7_4:1050051-1050070 Msa1134530:intron 25.0%
! TTGGATGTAATAATATGTGT+CGG + chr7_4:1050201-1050220 None:intergenic 25.0%
! TTGGATGTAATAATATGTGT+CGG + chr7_4:1050201-1050220 None:intergenic 25.0%
! GTATAGTTAATGATATAGGA+TGG + chr7_4:1050604-1050623 None:intergenic 25.0%
! ATCTTACAGAATACATCTAT+AGG - chr7_4:1050694-1050713 Msa1134530:intron 25.0%
! TAGATAAAATACCAACTATG+GGG + chr7_4:1050789-1050808 None:intergenic 25.0%
! CTAGATAAAATACCAACTAT+GGG + chr7_4:1050790-1050809 None:intergenic 25.0%
! ACTAGATAAAATACCAACTA+TGG + chr7_4:1050791-1050810 None:intergenic 25.0%
! AAAATTATCTAGTAGTGAAG+AGG - chr7_4:1050911-1050930 Msa1134530:CDS 25.0%
!! ATGTTTTTCTGTTAGTAAAC+TGG - chr7_4:1050938-1050957 Msa1134530:CDS 25.0%
AAATCAATAACATAATGGCG+GGG - chr7_4:1045730-1045749 Msa1134530:three_prime_UTR 30.0%
TCTTGTGTAAAAATAGGGTA+AGG + chr7_4:1046359-1046378 None:intergenic 30.0%
!!! TTACCCTATTTTTACACAAG+AGG - chr7_4:1046358-1046377 Msa1134530:intron 30.0%
TTCTAAGTTTCTAACAAGAG+AGG + chr7_4:1046454-1046473 None:intergenic 30.0%
!!! AGGTAGTTGTGATTAGTTTT+TGG - chr7_4:1046495-1046514 Msa1134530:intron 30.0%
TTAAGTTTGTAGGGGTAAAA+TGG - chr7_4:1046672-1046691 Msa1134530:intron 30.0%
ATAGAATGCTATAAGCCATA+AGG - chr7_4:1046826-1046845 Msa1134530:intron 30.0%
TTACTAAATAGACATCTAGC+TGG - chr7_4:1046862-1046881 Msa1134530:intron 30.0%
AACGTGAAGTGATTTATGTT+TGG - chr7_4:1046967-1046986 Msa1134530:intron 30.0%
AGTCGAAAGCTACAAATATT+AGG - chr7_4:1047044-1047063 Msa1134530:intron 30.0%
CGATGAGAAAAAGGAATAAT+AGG - chr7_4:1047246-1047265 Msa1134530:intron 30.0%
AGAAAAAGGAATAATAGGTG+TGG - chr7_4:1047251-1047270 Msa1134530:intron 30.0%
GATGAATTCTAATTGGATAC+TGG - chr7_4:1047414-1047433 Msa1134530:intron 30.0%
AACACTAGGTATCATCAAAA+GGG + chr7_4:1047479-1047498 None:intergenic 30.0%
AAACACTAGGTATCATCAAA+AGG + chr7_4:1047480-1047499 None:intergenic 30.0%
!! AATTGATGGTGTTTGGTAAA+TGG - chr7_4:1047569-1047588 Msa1134530:intron 30.0%
! TTTTATAGTGATGTCCAATG+AGG - chr7_4:1047753-1047772 Msa1134530:intron 30.0%
CAAAAATACTAGAACCTCAT+TGG + chr7_4:1047770-1047789 None:intergenic 30.0%
CGATAATGATGACTTCTAAT+TGG - chr7_4:1047828-1047847 Msa1134530:intron 30.0%
! AGTTTTACACTGATGTTCAA+CGG - chr7_4:1048029-1048048 Msa1134530:intron 30.0%
GTGTACTATTATGTAATGCT+TGG - chr7_4:1048506-1048525 Msa1134530:intron 30.0%
GTACTATTATGTAATGCTTG+GGG - chr7_4:1048508-1048527 Msa1134530:intron 30.0%
ATGAGTCATACTCAACTAAT+TGG - chr7_4:1048656-1048675 Msa1134530:intron 30.0%
TGAGTCATACTCAACTAATT+GGG - chr7_4:1048657-1048676 Msa1134530:intron 30.0%
AAGCTCTCTCATATAGATTA+CGG + chr7_4:1048714-1048733 None:intergenic 30.0%
AGAAACCACAAAATTTCTGA+TGG + chr7_4:1048843-1048862 None:intergenic 30.0%
GTGAAGCTTATATTAAAAGC+AGG - chr7_4:1048878-1048897 Msa1134530:intron 30.0%
TTGATCATGATAGACAAGAT+TGG + chr7_4:1049083-1049102 None:intergenic 30.0%
ATCAATCATGCAAACAGAAT+TGG - chr7_4:1049098-1049117 Msa1134530:intron 30.0%
!!! TTTTTTGTTATGCTAGGCTT+TGG - chr7_4:1049191-1049210 Msa1134530:intron 30.0%
CGGAAGTTATATTCTTTCTT+TGG - chr7_4:1049293-1049312 Msa1134530:intron 30.0%
!! TTTCTTTGGTCTTTCTTTTC+TGG - chr7_4:1049307-1049326 Msa1134530:intron 30.0%
TGAGTGTAATATCAGCAATA+AGG + chr7_4:1049631-1049650 None:intergenic 30.0%
CGGTATAGCTAAATATGTTA+AGG + chr7_4:1049861-1049880 None:intergenic 30.0%
GCTATACCGAATTATATCAT+TGG - chr7_4:1049872-1049891 Msa1134530:intron 30.0%
CCTTTCCCAATGATATAATT+CGG + chr7_4:1049881-1049900 None:intergenic 30.0%
CGAATTATATCATTGGGAAA+GGG - chr7_4:1049879-1049898 Msa1134530:intron 30.0%
TATTCCAGTGAAGCTATAAA+GGG + chr7_4:1049958-1049977 None:intergenic 30.0%
! TTGCTTCTTTGACTTTACTT+TGG - chr7_4:1050012-1050031 Msa1134530:intron 30.0%
GTTTATAGGATGAATGAATG+AGG - chr7_4:1050076-1050095 Msa1134530:intron 30.0%
!! ATGCCTTTAAAGTGAAATGT+TGG + chr7_4:1050170-1050189 None:intergenic 30.0%
! ATTTCACTTTAAAGGCATGT+CGG - chr7_4:1050172-1050191 Msa1134530:intron 30.0%
GTTAATAATGTATTGTCCTC+TGG - chr7_4:1050376-1050395 Msa1134530:intron 30.0%
ATTACATATAGAACATCCAG+AGG + chr7_4:1050395-1050414 None:intergenic 30.0%
CATAAATTCAGATCAGATCA+GGG + chr7_4:1050436-1050455 None:intergenic 30.0%
ACATAAATTCAGATCAGATC+AGG + chr7_4:1050437-1050456 None:intergenic 30.0%
!!! TATGTCATTTTGAGTTCTTC+AGG - chr7_4:1050452-1050471 Msa1134530:intron 30.0%
! TACTGAAGTTAGGTTCTTTT+TGG - chr7_4:1050633-1050652 Msa1134530:intron 30.0%
ATGTATTCTGTAAGATCATG+GGG + chr7_4:1050690-1050709 None:intergenic 30.0%
GATGTATTCTGTAAGATCAT+GGG + chr7_4:1050691-1050710 None:intergenic 30.0%
AGATGTATTCTGTAAGATCA+TGG + chr7_4:1050692-1050711 None:intergenic 30.0%
!!! GCTCTAAGAAATCTCAATTT+TGG - chr7_4:1050752-1050771 Msa1134530:CDS 30.0%
! AATTTCCGGTACTGAAATTT+GGG + chr7_4:1050848-1050867 None:intergenic 30.0%
! AAATTTCCGGTACTGAAATT+TGG + chr7_4:1050849-1050868 None:intergenic 30.0%
TCTTTGTCAGAAGAAATTTC+CGG + chr7_4:1050862-1050881 None:intergenic 30.0%
GTTTCAAGGAAAATAGCAGA+AGG + chr7_4:1045954-1045973 None:intergenic 35.0%
!!! TCTGCTATTTTCCTTGAAAC+TGG - chr7_4:1045954-1045973 Msa1134530:CDS 35.0%
AATCATATGTTACCTGCCAA+TGG + chr7_4:1046060-1046079 None:intergenic 35.0%
TCAACAACACTTCTAATGGA+AGG + chr7_4:1046220-1046239 None:intergenic 35.0%
AGCATCAACAACACTTCTAA+TGG + chr7_4:1046224-1046243 None:intergenic 35.0%
AGAATTAAAAAAGGGCAACC+CGG - chr7_4:1046267-1046286 Msa1134530:CDS 35.0%
!! AACTTATAGCGGATAGTGTT+TGG - chr7_4:1046573-1046592 Msa1134530:intron 35.0%
AGCTTATGATGTGAATGATG+AGG - chr7_4:1047334-1047353 Msa1134530:intron 35.0%
TGTGAATGATGAGGTCTAAT+TGG - chr7_4:1047343-1047362 Msa1134530:intron 35.0%
AGGAAGTAAGGATCAATTGA+TGG - chr7_4:1047555-1047574 Msa1134530:intron 35.0%
!! AAGGATCAATTGATGGTGTT+TGG - chr7_4:1047562-1047581 Msa1134530:intron 35.0%
GATCTGTTGACAGTGTAAAA+CGG - chr7_4:1047689-1047708 Msa1134530:intron 35.0%
! GTTTTACACTGATGTTCAAC+GGG - chr7_4:1048030-1048049 Msa1134530:intron 35.0%
ATAGGAAGTTAGGATTTAGC+AGG - chr7_4:1048222-1048241 Msa1134530:intron 35.0%
AGTTAGGATTTAGCAGGTTT+AGG - chr7_4:1048228-1048247 Msa1134530:intron 35.0%
GTTAGGATTTAGCAGGTTTA+GGG - chr7_4:1048229-1048248 Msa1134530:intron 35.0%
TTAGGATTTAGCAGGTTTAG+GGG - chr7_4:1048230-1048249 Msa1134530:intron 35.0%
CAAGTTGCGTTTAAATACTG+CGG - chr7_4:1048541-1048560 Msa1134530:intron 35.0%
GAGTCATACTCAACTAATTG+GGG - chr7_4:1048658-1048677 Msa1134530:intron 35.0%
AGTCATACTCAACTAATTGG+GGG - chr7_4:1048659-1048678 Msa1134530:intron 35.0%
! ATAGGCCATCAGAAATTTTG+TGG - chr7_4:1048835-1048854 Msa1134530:intron 35.0%
CCTTTCACAGAGACAATATT+AGG + chr7_4:1049135-1049154 None:intergenic 35.0%
CCTAATATTGTCTCTGTGAA+AGG - chr7_4:1049132-1049151 Msa1134530:intron 35.0%
TTGGTTTCTCATCCATAGTT+GGG - chr7_4:1049795-1049814 Msa1134530:intron 35.0%
CATGTAAGAAATCCCAACTA+TGG + chr7_4:1049810-1049829 None:intergenic 35.0%
CCGAATTATATCATTGGGAA+AGG - chr7_4:1049878-1049897 Msa1134530:intron 35.0%
CTATTCCAGTGAAGCTATAA+AGG + chr7_4:1049959-1049978 None:intergenic 35.0%
GCAATAACTTAAACTGGCAA+TGG + chr7_4:1049996-1050015 None:intergenic 35.0%
!! CCTTTAAAGTGAAATGTTGG+TGG + chr7_4:1050167-1050186 None:intergenic 35.0%
CCACCAACATTTCACTTTAA+AGG - chr7_4:1050164-1050183 Msa1134530:intron 35.0%
ATGCCTTAAATGTGAAGTGT+CGG + chr7_4:1050238-1050257 None:intergenic 35.0%
! ACTTCACATTTAAGGCATGT+CGG - chr7_4:1050240-1050259 Msa1134530:intron 35.0%
!! ATAAAGCACTGTCAAAGATC+TGG + chr7_4:1050328-1050347 None:intergenic 35.0%
AGATCAGGGAAGATAAAAAG+AGG + chr7_4:1050422-1050441 None:intergenic 35.0%
! AGTTCTTCAGGTTAGTATTC+AGG - chr7_4:1050464-1050483 Msa1134530:intron 35.0%
TTAATGATATAGGATGGCAC+AGG + chr7_4:1050598-1050617 None:intergenic 35.0%
TTTCTTCTTCCTACCATTGT+CGG - chr7_4:1050658-1050677 Msa1134530:intron 35.0%
!!! TCTTACTTTTTCCCCATAGT+TGG - chr7_4:1050775-1050794 Msa1134530:CDS 35.0%
ATATGATCGGAGTAGTAAGG+CGG + chr7_4:1045790-1045809 None:intergenic 40.0%
TTACTACTCCGATCATATGG+CGG - chr7_4:1045792-1045811 Msa1134530:three_prime_UTR 40.0%
GTGTGAATAGCGATGTAAAG+TGG + chr7_4:1045909-1045928 None:intergenic 40.0%
!! GAATAGAAGCACCAGTTTCA+AGG + chr7_4:1045968-1045987 None:intergenic 40.0%
ACTGGTGCTTCTATTCTCTT+TGG - chr7_4:1045972-1045991 Msa1134530:CDS 40.0%
GTGCTTCTATTCTCTTTGGT+TGG - chr7_4:1045976-1045995 Msa1134530:CDS 40.0%
GCTTCCTTATCTTTCTCCAT+TGG - chr7_4:1046041-1046060 Msa1134530:CDS 40.0%
CGTACAATACACCAAATGGT+GGG + chr7_4:1046333-1046352 None:intergenic 40.0%
CGGCCTCTTGTGTAAAAATA+GGG + chr7_4:1046364-1046383 None:intergenic 40.0%
ACGGCCTCTTGTGTAAAAAT+AGG + chr7_4:1046365-1046384 None:intergenic 40.0%
GATGTTGTCATGTGACTGTA+AGG + chr7_4:1046409-1046428 None:intergenic 40.0%
GGAGGCAGAATCAATTCTAT+CGG - chr7_4:1047086-1047105 Msa1134530:intron 40.0%
TAGTGTTAGAACAGCAAAGC+TGG - chr7_4:1048385-1048404 Msa1134530:intron 40.0%
TTACGTCTCCGTCAATAGAT+TGG - chr7_4:1048476-1048495 Msa1134530:intron 40.0%
CTCAGTAGCCAATCTATTGA+CGG + chr7_4:1048487-1048506 None:intergenic 40.0%
TAATAGGTCAAACCCTAGAG+CGG + chr7_4:1048621-1048640 None:intergenic 40.0%
ATACTCAACTAATTGGGGGA+TGG - chr7_4:1048663-1048682 Msa1134530:intron 40.0%
ACTATGGATGAGAAACCAAG+AGG + chr7_4:1049794-1049813 None:intergenic 40.0%
CTTGGTTTCTCATCCATAGT+TGG - chr7_4:1049794-1049813 Msa1134530:intron 40.0%
CATGGACTCAACATGCTTAA+AGG + chr7_4:1049935-1049954 None:intergenic 40.0%
!! AAAGTGAAATGTTGGTGGCT+CGG + chr7_4:1050162-1050181 None:intergenic 40.0%
! GGAAGTTTGTTCTTCTTGCT+CGG - chr7_4:1050566-1050585 Msa1134530:intron 40.0%
ACATCTATAGGAAGTCTTGC+TGG - chr7_4:1050706-1050725 Msa1134530:intron 40.0%
TTGAGATTTCTTAGAGCCAG+AGG + chr7_4:1050749-1050768 None:intergenic 40.0%
TTCGTCCCAAATTTCAGTAC+CGG - chr7_4:1050840-1050859 Msa1134530:CDS 40.0%
! TCAATAACATAATGGCGGGG+TGG - chr7_4:1045733-1045752 Msa1134530:three_prime_UTR 45.0%
GCCTTACTACTCCGATCATA+TGG - chr7_4:1045789-1045808 Msa1134530:three_prime_UTR 45.0%
GCCATATGATCGGAGTAGTA+AGG + chr7_4:1045793-1045812 None:intergenic 45.0%
TTGCTTCTCCGCCATATGAT+CGG + chr7_4:1045803-1045822 None:intergenic 45.0%
! TCATATGGCGGAGAAGCAAT+CGG - chr7_4:1045804-1045823 Msa1134530:three_prime_UTR 45.0%
GAAGCAATCGGAATCGGAAT+CGG - chr7_4:1045816-1045835 Msa1134530:three_prime_UTR 45.0%
AGAAAGATAAGGAAGCAGCG+AGG + chr7_4:1046037-1046056 None:intergenic 45.0%
CCTGCCAATGGAGAAAGATA+AGG + chr7_4:1046048-1046067 None:intergenic 45.0%
CCTTATCTTTCTCCATTGGC+AGG - chr7_4:1046045-1046064 Msa1134530:CDS 45.0%
GCGTACAATACACCAAATGG+TGG + chr7_4:1046334-1046353 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG + chr7_4:1046337-1046356 None:intergenic 45.0%
ACGGGTTTAAGTCCTGGAAA+CGG + chr7_4:1046384-1046403 None:intergenic 45.0%
TCATGTGACTGTAAGGTCAC+GGG + chr7_4:1046402-1046421 None:intergenic 45.0%
GTCATGTGACTGTAAGGTCA+CGG + chr7_4:1046403-1046422 None:intergenic 45.0%
CATCTTTACCAGTTGCGCTA+AGG - chr7_4:1046425-1046444 Msa1134530:intron 45.0%
!! TTAGCAGGTTTAGGGGTGTT+TGG - chr7_4:1048237-1048256 Msa1134530:intron 45.0%
GACGGAGACGTAAGCATAAT+TGG + chr7_4:1048469-1048488 None:intergenic 45.0%
CTAAAAGTAGGCAGACGGTT+CGG + chr7_4:1048587-1048606 None:intergenic 45.0%
TGGAGCTAAAAGTAGGCAGA+CGG + chr7_4:1048592-1048611 None:intergenic 45.0%
GAGCAAGTGGAGCTAAAAGT+AGG + chr7_4:1048599-1048618 None:intergenic 45.0%
AAAGGACTGCTGGGAACATT+AGG - chr7_4:1049150-1049169 Msa1134530:intron 45.0%
! TCTATGGTAATGAAGGGTGC+TGG - chr7_4:1049235-1049254 Msa1134530:intron 45.0%
CAGTGAAGCTATAAAGGGCA+TGG + chr7_4:1049953-1049972 None:intergenic 45.0%
CATGCCCTTTATAGCTTCAC+TGG - chr7_4:1049951-1049970 Msa1134530:intron 45.0%
!! GTCGGTGCTACAGGATAAAT+TGG + chr7_4:1050220-1050239 None:intergenic 45.0%
! GCACCGACACTTCACATTTA+AGG - chr7_4:1050232-1050251 Msa1134530:intron 45.0%
CAGGAATTCAACCTCAGCAA+TGG - chr7_4:1050483-1050502 Msa1134530:intron 45.0%
ATTCAACCTCAGCAATGGAC+TGG - chr7_4:1050488-1050507 Msa1134530:intron 45.0%
! TTCAACCTCAGCAATGGACT+GGG - chr7_4:1050489-1050508 Msa1134530:intron 45.0%
TCTGTTAGTAAACTGGTGCG+TGG - chr7_4:1050945-1050964 Msa1134530:CDS 45.0%
TCGATCGGTAAACTGAAGGA+CGG + chr7_4:1050985-1051004 None:intergenic 45.0%
CAGTTCGATCGGTAAACTGA+AGG + chr7_4:1050989-1051008 None:intergenic 45.0%
TACCGATCGAACTGTCTGTT+TGG - chr7_4:1050995-1051014 Msa1134530:CDS 45.0%
ACCGATCGAACTGTCTGTTT+GGG - chr7_4:1050996-1051015 Msa1134530:CDS 45.0%
TCCCAAACAGACAGTTCGAT+CGG + chr7_4:1051000-1051019 None:intergenic 45.0%
! ATAACATAATGGCGGGGTGG+TGG - chr7_4:1045736-1045755 Msa1134530:three_prime_UTR 50.0%
!! ATGAAGCGCTGACACAGACA+CGG - chr7_4:1046127-1046146 Msa1134530:CDS 50.0%
TTACACAAGAGGCCGTTTCC+AGG - chr7_4:1046369-1046388 Msa1134530:intron 50.0%
AAGGTCACGGGTTTAAGTCC+TGG + chr7_4:1046390-1046409 None:intergenic 50.0%
AGAGGTAACCTTAGCGCAAC+TGG + chr7_4:1046436-1046455 None:intergenic 50.0%
CTTATTCCCTCTGCTTCCTC+TGG + chr7_4:1049037-1049056 None:intergenic 50.0%
TGTCTCTGTGAAAGGACTGC+TGG - chr7_4:1049140-1049159 Msa1134530:intron 50.0%
GTCTCTGTGAAAGGACTGCT+GGG - chr7_4:1049141-1049160 Msa1134530:intron 50.0%
!! ATGTGAAGTGTCGGTGCTAC+AGG + chr7_4:1050229-1050248 None:intergenic 50.0%
CTCGCTCAACTCCATTTGCA+AGG + chr7_4:1050540-1050559 None:intergenic 50.0%
GCAAATGGAGTTGAGCGAGA+TGG - chr7_4:1050541-1050560 Msa1134530:intron 50.0%
ATGGAGTTGAGCGAGATGGT+TGG - chr7_4:1050545-1050564 Msa1134530:intron 50.0%
GGCGGAGAAGCAATCGGAAT+CGG - chr7_4:1045810-1045829 Msa1134530:three_prime_UTR 55.0%
ACTGAAGCTCCCGCATACAC+AGG - chr7_4:1046293-1046312 Msa1134530:intron 55.0%
CTGAAGCTCCCGCATACACA+GGG - chr7_4:1046294-1046313 Msa1134530:intron 55.0%
TTACCGGACCCTGTGTATGC+GGG + chr7_4:1046305-1046324 None:intergenic 55.0%
CTTACCGGACCCTGTGTATG+CGG + chr7_4:1046306-1046325 None:intergenic 55.0%
GCATACACAGGGTCCGGTAA+GGG - chr7_4:1046305-1046324 Msa1134530:intron 55.0%
CATACACAGGGTCCGGTAAG+GGG - chr7_4:1046306-1046325 Msa1134530:intron 55.0%
! CAAATGGTGGGACCCCTTAC+CGG + chr7_4:1046321-1046340 None:intergenic 55.0%
! AAAGTAGGCAGACGGTTCGG+CGG + chr7_4:1048584-1048603 None:intergenic 55.0%
AAACCCTAGAGCGGAGCAAG+TGG + chr7_4:1048612-1048631 None:intergenic 55.0%
! ACTGGCTTCTCCTGTACCAG+AGG - chr7_4:1049018-1049037 Msa1134530:intron 55.0%
TCCTGTACCAGAGGAAGCAG+AGG - chr7_4:1049027-1049046 Msa1134530:intron 55.0%
CCTGTACCAGAGGAAGCAGA+GGG - chr7_4:1049028-1049047 Msa1134530:intron 55.0%
TCTAGCCCAGTCCATTGCTG+AGG + chr7_4:1050497-1050516 None:intergenic 55.0%
!! CAGCAATGGACTGGGCTAGA+AGG - chr7_4:1050497-1050516 Msa1134530:intron 55.0%
GGGATGCATCCGACAATGGT+AGG + chr7_4:1050670-1050689 None:intergenic 55.0%
CATGGGGATGCATCCGACAA+TGG + chr7_4:1050674-1050693 None:intergenic 55.0%
! ACATAATGGCGGGGTGGTGG+TGG - chr7_4:1045739-1045758 Msa1134530:three_prime_UTR 60.0%
!! GGGGTGGTGGTGGAAAACAG+CGG - chr7_4:1045749-1045768 Msa1134530:three_prime_UTR 60.0%
ATGCGGGAGCTTCAGTGCAC+CGG + chr7_4:1046289-1046308 None:intergenic 60.0%
CGCATACACAGGGTCCGGTA+AGG - chr7_4:1046304-1046323 Msa1134530:intron 60.0%
CGGTAAGGGGTCCCACCATT+TGG - chr7_4:1046319-1046338 Msa1134530:intron 60.0%
AGCTCCACTTGCTCCGCTCT+AGG - chr7_4:1048605-1048624 Msa1134530:intron 60.0%
GCTCCACTTGCTCCGCTCTA+GGG - chr7_4:1048606-1048625 Msa1134530:intron 60.0%
CCCTCTGCTTCCTCTGGTAC+AGG + chr7_4:1049031-1049050 None:intergenic 60.0%
GCAGCTGTTGCTACAGCCTC+TGG - chr7_4:1050730-1050749 Msa1134530:CDS 60.0%
TGCGGGAGCTTCAGTGCACC+GGG + chr7_4:1046288-1046307 None:intergenic 65.0%
GCTCCCGCATACACAGGGTC+CGG - chr7_4:1046299-1046318 Msa1134530:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 1045630 1051147 1045630 ID=Msa1134530;Name=Msa1134530
chr7_4 mRNA 1045630 1051147 1045630 ID=Msa1134530-mRNA-1;Parent=Msa1134530;Name=Msa1134530-mRNA-1;_AED=0.01;_eAED=0.01;_QI=114|1|1|1|1|1|2|235|253
chr7_4 exon 1045630 1046303 1045630 ID=Msa1134530-mRNA-1:exon:369;Parent=Msa1134530-mRNA-1
chr7_4 exon 1050711 1051147 1050711 ID=Msa1134530-mRNA-1:exon:368;Parent=Msa1134530-mRNA-1
chr7_4 five_prime_UTR 1051034 1051147 1051034 ID=Msa1134530-mRNA-1:five_prime_utr;Parent=Msa1134530-mRNA-1
chr7_4 CDS 1050711 1051033 1050711 ID=Msa1134530-mRNA-1:cds;Parent=Msa1134530-mRNA-1
chr7_4 CDS 1045865 1046303 1045865 ID=Msa1134530-mRNA-1:cds;Parent=Msa1134530-mRNA-1
chr7_4 three_prime_UTR 1045630 1045864 1045630 ID=Msa1134530-mRNA-1:three_prime_utr;Parent=Msa1134530-mRNA-1
Gene Sequence

>Msa1134530

ATGGCGGGGTGGTGGTGGAAAACAGCGGTGTTAATAGCAATATCCGCCTTACTACTCCGATCATATGGCGGAGAAGCAATCGGAATCGGAATCGGAATCGATAAAGAATCATTGATGAAATGGATGAGAGATTTATCTGATAAACTAGGAATTTGGGCGATTCCACTTTACATCGCTATTCACACAATTTCAATTGCTCTTTGTTTACCTTCTGCTATTTTCCTTGAAACTGGTGCTTCTATTCTCTTTGGTTGGTTTGCTTCTGTTCTTTGTGTTTTCTCTGCTAAGATCCTCGCTGCTTCCTTATCTTTCTCCATTGGCAGGTTAGTATTCAGGAATTCAACCTCAGCAATGGACTGGGCTAGAAGGAACAAATATTTTAAAATCCTTGCAAATGGAGTTGAGCGAGATGGTTGGAAGTTTGTTCTTCTTGCTCGGTTCTCGCCTGTGCCATCCTATATCATTAACTATACCTTAGCTGCTACTGAAGTTAGGTTCTTTTTGGATTTTCTTCTTCCTACCATTGTCGGATGCATCCCCATGATCTTACAGAATACATCTATAGGAAGTCTTGCTGGTGCAGCTGTTGCTACAGCCTCTGGCTCTAAGAAATCTCAATTTTGGTCTTACTTTTTCCCCATAGTTGGTATTTTATCTAGTGTACTTATTTCTTTGAGAATTAAAAAGTATTCGTCCCAAATTTCAGTACCGGAAATTTCTTCTGACAAAGATAACACTGTTGAGTCAAAACAACAAAAATAA
Protein sequence

>Msa1134530

MAGWWWKTAVLIAISALLLRSYGGEAIGIGIGIDKESLMKWMRDLSDKLGIWAIPLYIAIHTISIALCLPSAIFLETGASILFGWFASVLCVFSAKILAASLSFSIGRLVFRNSTSAMDWARRNKYFKILANGVERDGWKFVLLARFSPVPSYIINYTLAATEVRFFLDFLLPTIVGCIPMILQNTSIGSLAGAAVATASGSKKSQFWSYFFPIVGILSSVLISLRIKKYSSQISVPEISSDKDNTVESKQQK*