AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1004710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1004710 MtrunA17_Chr7g0214791 96.209 211 6 1 1 211 1 209 4.18e-143 404
Msa1004710 MtrunA17_Chr7g0214791 100.000 145 0 0 109 253 207 351 4.85e-99 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1004710 AT1G44960.1 67.299 211 67 1 32 242 26 234 1.88e-103 301

Find 70 sgRNAs with CRISPR-Local

Find 296 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTCCGGTACTGAAATT+TGG 0.132573 7_1:+2305136 None:intergenic
TATCTGATAAACTAGGAATT+TGG 0.145494 7_1:-2310328 Msa1004710:CDS
GCTCTAAGAAATCTCAATTT+TGG 0.229017 7_1:-2305230 Msa1004710:CDS
AGTTCTTCAGGTTAGTATTC+AGG 0.245543 7_1:-2305518 Msa1004710:intron
ACTGGTGCTTCTCTTCTCTT+TGG 0.275215 7_1:-2310233 Msa1004710:CDS
ACTAGATAAAATACCAACTA+TGG 0.282343 7_1:+2305194 None:intergenic
AATACTAACAACAACAAAAT+TGG 0.287741 7_1:+2304929 None:intergenic
ATCTGATAAACTAGGAATTT+GGG 0.313710 7_1:-2310327 Msa1004710:CDS
GACTTGATCTTCATTTAATC+TGG 0.331073 7_1:-2304857 Msa1004710:three_prime_UTR
GAATTAAATCAATAACATTA+TGG 0.336357 7_1:-2310480 Msa1004710:exon
AATTTCCGGTACTGAAATTT+GGG 0.342524 7_1:+2305137 None:intergenic
CCTTAGCTGCTACTGAAGTT+AGG 0.357035 7_1:-2305359 Msa1004710:CDS
GCAGCTGTTGCTACAGCCTC+TGG 0.372165 7_1:-2305252 Msa1004710:CDS
ATAAAGAATCATTGATGAAA+TGG 0.374496 7_1:-2310361 Msa1004710:CDS
TACCGATCGAACTGTCTGTT+TGG 0.377737 7_1:-2304987 Msa1004710:three_prime_UTR
TCTTTGTCAGAAGAAATTTC+CGG 0.385161 7_1:+2305123 None:intergenic
GTGCTTCTCTTCTCTTTGGT+TGG 0.388199 7_1:-2310229 Msa1004710:CDS
CAGCAATGGACTGGGCTAGA+AGG 0.407462 7_1:-2305485 Msa1004710:CDS
ACCGATCGAACTGTCTGTTT+GGG 0.409473 7_1:-2304986 Msa1004710:three_prime_UTR
CCTTATCTTTCTCCATTGGC+AGG 0.426737 7_1:-2310160 Msa1004710:intron
TTTCTTCTTCCTACCATTGT+CGG 0.428961 7_1:-2305324 Msa1004710:CDS
GGAAGTTTGTTCTTCTTGCT+CGG 0.432228 7_1:-2305416 Msa1004710:CDS
ACATCTATAGGAAGTCTTGC+TGG 0.441068 7_1:-2305276 Msa1004710:CDS
TAAGGTATAGTTAATGATAT+AGG 0.443398 7_1:+2305377 None:intergenic
ACATTATGGCGGGGTGGTGG+TGG 0.449582 7_1:-2310466 Msa1004710:exon
GGCGGAGAAGCAATCGGAAT+CGG 0.453699 7_1:-2310395 Msa1004710:CDS
GCCTTACTACTCCGATCATA+TGG 0.458022 7_1:-2310416 Msa1004710:CDS
TAAATCAATAACATTATGGC+GGG 0.458116 7_1:-2310476 Msa1004710:exon
GCTTCCTTATCTTTCTCCAT+TGG 0.464011 7_1:-2310164 Msa1004710:CDS
ATCTTACAGAATACATCTAT+AGG 0.469430 7_1:-2305288 Msa1004710:CDS
AGATGTATTCTGTAAGATCA+TGG 0.472448 7_1:+2305293 None:intergenic
TTCGTCCCAAATTTCAGTAC+CGG 0.474329 7_1:-2305142 Msa1004710:CDS
ATTCAACCTCAGCAATGGAC+TGG 0.500375 7_1:-2305494 Msa1004710:CDS
TCATATGGCGGAGAAGCAAT+CGG 0.500801 7_1:-2310401 Msa1004710:CDS
TTCAACCTCAGCAATGGACT+GGG 0.502524 7_1:-2305493 Msa1004710:CDS
GCCATATGATCGGAGTAGTA+AGG 0.504927 7_1:+2310415 None:intergenic
CCTAACTTCAGTAGCAGCTA+AGG 0.506076 7_1:+2305359 None:intergenic
GATGTATTCTGTAAGATCAT+GGG 0.518629 7_1:+2305294 None:intergenic
CTAGATAAAATACCAACTAT+GGG 0.519361 7_1:+2305195 None:intergenic
CCTGCCAATGGAGAAAGATA+AGG 0.520262 7_1:+2310160 None:intergenic
ATAACATTATGGCGGGGTGG+TGG 0.525274 7_1:-2310469 Msa1004710:exon
TTGCTTCTCCGCCATATGAT+CGG 0.526914 7_1:+2310405 None:intergenic
CTCGCTCAACTCCATTTGCA+AGG 0.541452 7_1:+2305445 None:intergenic
GTATGAATAGCGATGTATAG+TGG 0.550765 7_1:+2310299 None:intergenic
AAAATTATCTAGTAGTGAAG+AGG 0.553803 7_1:-2305071 Msa1004710:three_prime_UTR
AATCATATGTTACCTGCCAA+TGG 0.556218 7_1:+2310148 None:intergenic
AGAGATTTATCTGATAAACT+AGG 0.558993 7_1:-2310335 Msa1004710:CDS
GAAGCAATCGGAATCGGAGT+CGG 0.563801 7_1:-2310389 Msa1004710:CDS
GGGATGCATCCGACAATGGT+AGG 0.579071 7_1:+2305315 None:intergenic
TTGAGATTTCTTAGAGCCAG+AGG 0.582555 7_1:+2305236 None:intergenic
TCTAGCCCAGTCCATTGCTG+AGG 0.592500 7_1:+2305488 None:intergenic
ATGGAGTTGAGCGAGATGGT+TGG 0.593190 7_1:-2305437 Msa1004710:CDS
CAGGAATTCAACCTCAGCAA+TGG 0.593899 7_1:-2305499 Msa1004710:CDS
TCCCAAACAGACAGTTCGAT+CGG 0.605038 7_1:+2304985 None:intergenic
CATGGGGATGCATCCGACAA+TGG 0.608315 7_1:+2305311 None:intergenic
ATGTATTCTGTAAGATCATG+GGG 0.610828 7_1:+2305295 None:intergenic
TTAAATCAATAACATTATGG+CGG 0.617001 7_1:-2310477 Msa1004710:exon
TCTGTTAGTAAACTGGTGCG+TGG 0.633463 7_1:-2305037 Msa1004710:three_prime_UTR
GTATAGTTAATGATATAGGA+TGG 0.638706 7_1:+2305381 None:intergenic
GCAAATGGAGTTGAGCGAGA+TGG 0.646060 7_1:-2305441 Msa1004710:CDS
CAGTTCGATCGGTAAACTGA+AGG 0.647256 7_1:+2304996 None:intergenic
AAATCAATAACATTATGGCG+GGG 0.648875 7_1:-2310475 Msa1004710:exon
TTAATGATATAGGATGGCAC+AGG 0.650154 7_1:+2305387 None:intergenic
GGGGTGGTGGTGGAAAACAG+CGG 0.679901 7_1:-2310456 Msa1004710:CDS
TAGATAAAATACCAACTATG+GGG 0.680141 7_1:+2305196 None:intergenic
TCAATAACATTATGGCGGGG+TGG 0.684770 7_1:-2310472 Msa1004710:exon
TCGATCGGTAAACTGAAGGA+CGG 0.705675 7_1:+2305000 None:intergenic
TTACTACTCCGATCATATGG+CGG 0.710739 7_1:-2310413 Msa1004710:CDS
ATATGATCGGAGTAGTAAGG+CGG 0.729764 7_1:+2310418 None:intergenic
AGAAAGATAAGGAAGCAGCG+AGG 0.748647 7_1:+2310171 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAGTTGATTTAATAATTA+AGG + chr7_1:2306964-2306983 None:intergenic 10.0%
!!! TTAATTTATTATGTTGTTTT+AGG - chr7_1:2306982-2307001 Msa1004710:intron 10.0%
!! TTAATTCATTAAATTGTTAT+AGG - chr7_1:2307653-2307672 Msa1004710:intron 10.0%
!! TAGTCATTAATATTAATAAT+AGG + chr7_1:2308086-2308105 None:intergenic 10.0%
!! AAATAAACATGTTTAAATTT+AGG - chr7_1:2308917-2308936 Msa1004710:intron 10.0%
!!! AATTTTTCAACAAAATTTTA+AGG + chr7_1:2309017-2309036 None:intergenic 10.0%
!! GAATTAAATCAATAACATTA+TGG - chr7_1:2304948-2304967 Msa1004710:three_prime_UTR 15.0%
!!! TTTCTTTTTCACATTTTTAT+CGG + chr7_1:2305387-2305406 None:intergenic 15.0%
!! TAGAAACTTAGAATTAAAAA+AGG - chr7_1:2305481-2305500 Msa1004710:CDS 15.0%
!! AGAAACTTAGAATTAAAAAA+GGG - chr7_1:2305482-2305501 Msa1004710:CDS 15.0%
!!! TAGAATTAGTTGAATAATTT+TGG - chr7_1:2305692-2305711 Msa1004710:intron 15.0%
!!! TAGAATTAGTTGAATAATTT+TGG - chr7_1:2305692-2305711 Msa1004710:intron 15.0%
!!! TGCATTTAATTAATTTTTGT+AGG - chr7_1:2305869-2305888 Msa1004710:intron 15.0%
!!! GCATTTAATTAATTTTTGTA+GGG - chr7_1:2306111-2306130 Msa1004710:intron 15.0%
!!! CATTTAATTAATTTTTGTAG+GGG - chr7_1:2306112-2306131 Msa1004710:intron 15.0%
!! TATATTATTAAATGTGTGTT+TGG - chr7_1:2306334-2306353 Msa1004710:intron 15.0%
!!! TGATTTTGTAAATTTGATTT+TGG - chr7_1:2306389-2306408 Msa1004710:intron 15.0%
!!! GAATAAATTTGAGTTAAATA+TGG - chr7_1:2306462-2306481 Msa1004710:intron 15.0%
!!! AATTTTTGTTTTTTCAAAAG+TGG - chr7_1:2306601-2306620 Msa1004710:intron 15.0%
!! TTAAATAAACTAATTGTGTT+TGG - chr7_1:2307068-2307087 Msa1004710:intron 15.0%
!!! TTTTGTAGTTTTTTAAATTG+TGG - chr7_1:2307232-2307251 Msa1004710:intron 15.0%
!! AAAAAAATACAAAGAATTTG+AGG - chr7_1:2307796-2307815 Msa1004710:intron 15.0%
!! TTAAATCAATAACATTATGG+CGG - chr7_1:2304951-2304970 Msa1004710:three_prime_UTR 20.0%
!! ATAAAGAATCATTGATGAAA+TGG - chr7_1:2305067-2305086 Msa1004710:three_prime_UTR 20.0%
!!! TAGTTGAATAATTTTGGTTA+AGG - chr7_1:2305698-2305717 Msa1004710:intron 20.0%
!!! TAGTTGAATAATTTTGGTTA+AGG - chr7_1:2305698-2305717 Msa1004710:intron 20.0%
!!! GGTTTAAGTTTAATCAATTA+TGG - chr7_1:2306513-2306532 Msa1004710:intron 20.0%
!! TTAAGTTTAATCAATTATGG+AGG - chr7_1:2306516-2306535 Msa1004710:intron 20.0%
!!! AAAACAAAAATTGATTCTAG+AGG + chr7_1:2306593-2306612 None:intergenic 20.0%
!! TAATTGGATGTAAATTAGTT+TGG - chr7_1:2306807-2306826 Msa1004710:intron 20.0%
!! ATGATATGATGAATTCTAAT+TGG - chr7_1:2306855-2306874 Msa1004710:intron 20.0%
!! AAATTAACAACTAAAATGCT+AGG + chr7_1:2307360-2307379 None:intergenic 20.0%
!! AAACTATCAAATAAAACTCT+TGG + chr7_1:2307613-2307632 None:intergenic 20.0%
!! TTAAATTGTTATAGGAAGTT+AGG - chr7_1:2307661-2307680 Msa1004710:intron 20.0%
!! CTTAATAAACTATTTGTGTT+TGG - chr7_1:2307745-2307764 Msa1004710:intron 20.0%
!! CATACATGTTTATATCATTA+AGG - chr7_1:2308229-2308248 Msa1004710:intron 20.0%
!! AATCTTTGTAATCAAATGAT+TGG - chr7_1:2308419-2308438 Msa1004710:intron 20.0%
!!! TTGTTTTTATTTGTTATGCT+AGG - chr7_1:2308633-2308652 Msa1004710:intron 20.0%
!!! AAATATTCTATGGTAATGAA+GGG - chr7_1:2308677-2308696 Msa1004710:intron 20.0%
!!! GCTTATTTTTGTTTTGTATA+CGG - chr7_1:2308721-2308740 Msa1004710:intron 20.0%
!! TGAGTTAAAATTGATAGAAT+AGG - chr7_1:2308825-2308844 Msa1004710:intron 20.0%
!! TGTTGAAAAATTTGATAAGT+TGG - chr7_1:2309025-2309044 Msa1004710:intron 20.0%
!!! CTTCTAAAATAAAAAATCAC+AGG + chr7_1:2309132-2309151 None:intergenic 20.0%
!! TTTATAAAAATGTATCCTCT+TGG - chr7_1:2309222-2309241 Msa1004710:intron 20.0%
!! AATTATAAGTGGTAGTTTAT+AGG - chr7_1:2309508-2309527 Msa1004710:intron 20.0%
!! ATTTGAGAAAATGGATAAAT+TGG + chr7_1:2309734-2309753 None:intergenic 20.0%
!! GTGATAAATATTTGAGAAAA+TGG + chr7_1:2309743-2309762 None:intergenic 20.0%
!! TTTATTAGTAACAACTAACA+AGG - chr7_1:2309789-2309808 Msa1004710:intron 20.0%
!! GACAATACATTATTAACATA+TGG + chr7_1:2309819-2309838 None:intergenic 20.0%
!!! TATTTTAAAATCCTTGCAAA+TGG - chr7_1:2309972-2309991 Msa1004710:intron 20.0%
!! TAAGGTATAGTTAATGATAT+AGG + chr7_1:2310054-2310073 None:intergenic 20.0%
!! AATACTAACAACAACAAAAT+TGG + chr7_1:2310502-2310521 None:intergenic 20.0%
!! AAGAGTATAGTCAACAAAAA+CGG - chr7_1:2304876-2304895 Msa1004710:three_prime_UTR 25.0%
! TAAATCAATAACATTATGGC+GGG - chr7_1:2304952-2304971 Msa1004710:three_prime_UTR 25.0%
! AGAGATTTATCTGATAAACT+AGG - chr7_1:2305093-2305112 Msa1004710:CDS 25.0%
! TATCTGATAAACTAGGAATT+TGG - chr7_1:2305100-2305119 Msa1004710:CDS 25.0%
! ATCTGATAAACTAGGAATTT+GGG - chr7_1:2305101-2305120 Msa1004710:CDS 25.0%
!!! GTTTTTGGTAAAAAAAGTAG+CGG - chr7_1:2305733-2305752 Msa1004710:intron 25.0%
! ACTGATAAGATAGTTTATAG+TGG - chr7_1:2305759-2305778 Msa1004710:intron 25.0%
! ACTTATAAGCTAACTTATAG+CGG - chr7_1:2305785-2305804 Msa1004710:intron 25.0%
!!! GTTTTTGGTAAAAAAAGTAG+CGG - chr7_1:2305733-2305752 Msa1004710:intron 25.0%
! ACTGATAAGATAGTTTATAG+TGG - chr7_1:2305759-2305778 Msa1004710:intron 25.0%
! ACTTATAAGCTAACTTATAG+CGG - chr7_1:2305785-2305804 Msa1004710:intron 25.0%
!! TAATTTTTGTAGGGGTAAAA+TGG - chr7_1:2306120-2306139 Msa1004710:intron 25.0%
!! TTATTTTCATGAGCTTATAG+AGG + chr7_1:2306194-2306213 None:intergenic 25.0%
! AAGACATAAAATTGACCTTA+TGG + chr7_1:2306292-2306311 None:intergenic 25.0%
! CGATAAATTTAGTGTATGTT+TGG + chr7_1:2306629-2306648 None:intergenic 25.0%
!!! ATTTTTTGTCGATGAGAAAA+AGG - chr7_1:2306685-2306704 Msa1004710:intron 25.0%
!!! TGTAGTTATTTGTTATGTTG+TGG - chr7_1:2306748-2306767 Msa1004710:intron 25.0%
! AAATTGACAACTAAAACACT+AGG + chr7_1:2306940-2306959 None:intergenic 25.0%
!!! TTATGTTGTTTTAGGAAGTA+AGG - chr7_1:2306990-2307009 Msa1004710:intron 25.0%
! TGTTGTTATAAAGTGAGTTT+AGG - chr7_1:2307530-2307549 Msa1004710:intron 25.0%
! GTTTATTAAGTTAAAGAGCT+TGG + chr7_1:2307735-2307754 None:intergenic 25.0%
! AAAATACAAAGAATTTGAGG+TGG - chr7_1:2307799-2307818 Msa1004710:intron 25.0%
! TGTACTATTATGTAATGCTT+GGG - chr7_1:2307956-2307975 Msa1004710:intron 25.0%
!!! TCTTTTGTATGTCGATTTAT+AGG - chr7_1:2308265-2308284 Msa1004710:intron 25.0%
!! AAATTTTGTGGTTTCTTTGT+TGG - chr7_1:2308295-2308314 Msa1004710:intron 25.0%
! TGAAGCTTATATTAAAAGCA+GGG - chr7_1:2308327-2308346 Msa1004710:intron 25.0%
! TCATTTGATTACAAAGATTG+AGG + chr7_1:2308418-2308437 None:intergenic 25.0%
! TTTGTAATCAAATGATTGGT+TGG - chr7_1:2308423-2308442 Msa1004710:intron 25.0%
!! TAATTTGAGATGAAAGTTAC+TGG - chr7_1:2308448-2308467 Msa1004710:intron 25.0%
! TAGAATATTTGCACAGAAAA+AGG + chr7_1:2308667-2308686 None:intergenic 25.0%
!! TTTTCTGTGCAAATATTCTA+TGG - chr7_1:2308667-2308686 Msa1004710:intron 25.0%
!! CAAATATTCTATGGTAATGA+AGG - chr7_1:2308676-2308695 Msa1004710:intron 25.0%
!! AGTTTTGCATCTTCATTTTA+CGG - chr7_1:2308857-2308876 Msa1004710:intron 25.0%
!!! AAATTTGATAAGTTGGTTGA+AGG - chr7_1:2309032-2309051 Msa1004710:intron 25.0%
! CTATACCGAATTATATCATT+GGG - chr7_1:2309319-2309338 Msa1004710:intron 25.0%
! ATATATTGTCTGATTCAAGT+TGG - chr7_1:2309355-2309374 Msa1004710:intron 25.0%
! AAAGAAGCAATAACTTAAAC+TGG + chr7_1:2309448-2309467 None:intergenic 25.0%
! GTCATTGTATGAATTATAAG+TGG - chr7_1:2309497-2309516 Msa1004710:intron 25.0%
! TTGGATGTAATAATATGTGT+CGG + chr7_1:2309647-2309666 None:intergenic 25.0%
! TTGGATGTAATAATATGTGT+CGG + chr7_1:2309647-2309666 None:intergenic 25.0%
! GTATAGTTAATGATATAGGA+TGG + chr7_1:2310050-2310069 None:intergenic 25.0%
! ATCTTACAGAATACATCTAT+AGG - chr7_1:2310140-2310159 Msa1004710:intron 25.0%
! TAGATAAAATACCAACTATG+GGG + chr7_1:2310235-2310254 None:intergenic 25.0%
! CTAGATAAAATACCAACTAT+GGG + chr7_1:2310236-2310255 None:intergenic 25.0%
! ACTAGATAAAATACCAACTA+TGG + chr7_1:2310237-2310256 None:intergenic 25.0%
! AAAATTATCTAGTAGTGAAG+AGG - chr7_1:2310357-2310376 Msa1004710:CDS 25.0%
!! ATGTTTTTCTGTTAGTAAAC+TGG - chr7_1:2310384-2310403 Msa1004710:CDS 25.0%
AAATCAATAACATTATGGCG+GGG - chr7_1:2304953-2304972 Msa1004710:three_prime_UTR 30.0%
GTTTCAAGAAAAATAGCAGA+AGG + chr7_1:2305177-2305196 None:intergenic 30.0%
!!! TCTGCTATTTTTCTTGAAAC+TGG - chr7_1:2305177-2305196 Msa1004710:CDS 30.0%
TTCTAAGTTTCTAACAAGAG+AGG + chr7_1:2305677-2305696 None:intergenic 30.0%
!!! AGGTAGTTGTGATTAGTTTT+TGG - chr7_1:2305718-2305737 Msa1004710:intron 30.0%
TTCTAAGTTTCTAACAAGAG+AGG + chr7_1:2305677-2305696 None:intergenic 30.0%
!!! AGGTAGTTGTGATTAGTTTT+TGG - chr7_1:2305718-2305737 Msa1004710:intron 30.0%
ATAGAATGCTATAAGCCATA+AGG - chr7_1:2306274-2306293 Msa1004710:intron 30.0%
TTACTAAATAGACATCTAGC+TGG - chr7_1:2306310-2306329 Msa1004710:intron 30.0%
AACGTGAAGTGATTTATGTT+TGG - chr7_1:2306415-2306434 Msa1004710:intron 30.0%
AGTCGAAAGCTACAAATATT+AGG - chr7_1:2306492-2306511 Msa1004710:intron 30.0%
CGATGAGAAAAAGGAATAAT+AGG - chr7_1:2306694-2306713 Msa1004710:intron 30.0%
AGAAAAAGGAATAATAGGTG+TGG - chr7_1:2306699-2306718 Msa1004710:intron 30.0%
GATGAATTCTAATTGGATAC+TGG - chr7_1:2306862-2306881 Msa1004710:intron 30.0%
AAACACTAGGTATCATCAAA+GGG + chr7_1:2306927-2306946 None:intergenic 30.0%
AAAACACTAGGTATCATCAA+AGG + chr7_1:2306928-2306947 None:intergenic 30.0%
!! AATTGATGGTGTTTGGTAAA+TGG - chr7_1:2307016-2307035 Msa1004710:intron 30.0%
! TTTTATAGTGATGTCCAATG+AGG - chr7_1:2307200-2307219 Msa1004710:intron 30.0%
CAAAAATACTAGAACCTCAT+TGG + chr7_1:2307217-2307236 None:intergenic 30.0%
CGATAATGATGACTTCTAAT+TGG - chr7_1:2307275-2307294 Msa1004710:intron 30.0%
! AGTTTTACACTGATGTTCAA+CGG - chr7_1:2307476-2307495 Msa1004710:intron 30.0%
GTGTACTATTATGTAATGCT+TGG - chr7_1:2307955-2307974 Msa1004710:intron 30.0%
GTACTATTATGTAATGCTTG+GGG - chr7_1:2307957-2307976 Msa1004710:intron 30.0%
ATGAGTCATACTCAACTAAT+TGG - chr7_1:2308105-2308124 Msa1004710:intron 30.0%
TGAGTCATACTCAACTAATT+GGG - chr7_1:2308106-2308125 Msa1004710:intron 30.0%
AAGCTCTCTCATATAGATTA+CGG + chr7_1:2308163-2308182 None:intergenic 30.0%
AGAAACCACAAAATTTCTGA+TGG + chr7_1:2308291-2308310 None:intergenic 30.0%
GTGAAGCTTATATTAAAAGC+AGG - chr7_1:2308326-2308345 Msa1004710:intron 30.0%
TTGATCATGATAGACAAGAT+TGG + chr7_1:2308531-2308550 None:intergenic 30.0%
ATCAATCATGCAAACAGAAT+TGG - chr7_1:2308546-2308565 Msa1004710:intron 30.0%
!! TTATTTGTTATGCTAGGCTT+TGG - chr7_1:2308639-2308658 Msa1004710:intron 30.0%
CGGAAGTTATATTCTTTCTT+TGG - chr7_1:2308741-2308760 Msa1004710:intron 30.0%
!! TTTCTTTGGTCTTTCTTTTC+TGG - chr7_1:2308755-2308774 Msa1004710:intron 30.0%
TGAGTGTAATATCAGCAATA+AGG + chr7_1:2309079-2309098 None:intergenic 30.0%
CGGTATAGCTAAATATGTTA+AGG + chr7_1:2309307-2309326 None:intergenic 30.0%
GCTATACCGAATTATATCAT+TGG - chr7_1:2309318-2309337 Msa1004710:intron 30.0%
CCTTTCCCAATGATATAATT+CGG + chr7_1:2309327-2309346 None:intergenic 30.0%
CGAATTATATCATTGGGAAA+GGG - chr7_1:2309325-2309344 Msa1004710:intron 30.0%
TATTCCAGTGAAGCTATAAA+GGG + chr7_1:2309404-2309423 None:intergenic 30.0%
! TTGCTTCTTTGACTTTACTT+TGG - chr7_1:2309458-2309477 Msa1004710:intron 30.0%
GTTTATAGGATGAATGAATG+AGG - chr7_1:2309522-2309541 Msa1004710:intron 30.0%
!! ATGCCTTTAAAGTGAAATGT+TGG + chr7_1:2309616-2309635 None:intergenic 30.0%
! ATTTCACTTTAAAGGCATGT+CGG - chr7_1:2309618-2309637 Msa1004710:intron 30.0%
GTTAATAATGTATTGTCCTC+TGG - chr7_1:2309822-2309841 Msa1004710:intron 30.0%
CATAAATTCAGATCAGATCA+GGG + chr7_1:2309882-2309901 None:intergenic 30.0%
ACATAAATTCAGATCAGATC+AGG + chr7_1:2309883-2309902 None:intergenic 30.0%
!!! TATGTCATTTTGAGTTCTTC+AGG - chr7_1:2309898-2309917 Msa1004710:intron 30.0%
! TACTGAAGTTAGGTTCTTTT+TGG - chr7_1:2310079-2310098 Msa1004710:intron 30.0%
ATGTATTCTGTAAGATCATG+GGG + chr7_1:2310136-2310155 None:intergenic 30.0%
GATGTATTCTGTAAGATCAT+GGG + chr7_1:2310137-2310156 None:intergenic 30.0%
AGATGTATTCTGTAAGATCA+TGG + chr7_1:2310138-2310157 None:intergenic 30.0%
!!! GCTCTAAGAAATCTCAATTT+TGG - chr7_1:2310198-2310217 Msa1004710:CDS 30.0%
! AATTTCCGGTACTGAAATTT+GGG + chr7_1:2310294-2310313 None:intergenic 30.0%
! AAATTTCCGGTACTGAAATT+TGG + chr7_1:2310295-2310314 None:intergenic 30.0%
TCTTTGTCAGAAGAAATTTC+CGG + chr7_1:2310308-2310327 None:intergenic 30.0%
GTATGAATAGCGATGTATAG+TGG + chr7_1:2305132-2305151 None:intergenic 35.0%
AATCATATGTTACCTGCCAA+TGG + chr7_1:2305283-2305302 None:intergenic 35.0%
TCAACAACACTTCTAATGGA+AGG + chr7_1:2305443-2305462 None:intergenic 35.0%
AGCATCAACAACACTTCTAA+TGG + chr7_1:2305447-2305466 None:intergenic 35.0%
AGAATTAAAAAAGGGCAACC+CGG - chr7_1:2305490-2305509 Msa1004710:CDS 35.0%
TCTTGTGTAAAAACAGGGTA+AGG + chr7_1:2305582-2305601 None:intergenic 35.0%
!!! TTACCCTGTTTTTACACAAG+AGG - chr7_1:2305581-2305600 Msa1004710:intron 35.0%
!! AACTTATAGCGGATAGTGTT+TGG - chr7_1:2305796-2305815 Msa1004710:intron 35.0%
!! AACTTATAGCGGATAGTGTT+TGG - chr7_1:2305796-2305815 Msa1004710:intron 35.0%
AGCTTATGATGTGAATGATG+AGG - chr7_1:2306782-2306801 Msa1004710:intron 35.0%
TGTGAATGATGAGGTCTAAT+TGG - chr7_1:2306791-2306810 Msa1004710:intron 35.0%
AGGAAGTAAGGATCAATTGA+TGG - chr7_1:2307002-2307021 Msa1004710:intron 35.0%
!! AAGGATCAATTGATGGTGTT+TGG - chr7_1:2307009-2307028 Msa1004710:intron 35.0%
GATCTGTTGACAGTGTAAAA+CGG - chr7_1:2307136-2307155 Msa1004710:intron 35.0%
! GTTTTACACTGATGTTCAAC+GGG - chr7_1:2307477-2307496 Msa1004710:intron 35.0%
ATAGGAAGTTAGGATTTAGC+AGG - chr7_1:2307671-2307690 Msa1004710:intron 35.0%
AGTTAGGATTTAGCAGGTTT+AGG - chr7_1:2307677-2307696 Msa1004710:intron 35.0%
GTTAGGATTTAGCAGGTTTA+GGG - chr7_1:2307678-2307697 Msa1004710:intron 35.0%
TTAGGATTTAGCAGGTTTAG+GGG - chr7_1:2307679-2307698 Msa1004710:intron 35.0%
CAAGTTGCGTTTAAATACTG+CGG - chr7_1:2307990-2308009 Msa1004710:intron 35.0%
GAGTCATACTCAACTAATTG+GGG - chr7_1:2308107-2308126 Msa1004710:intron 35.0%
AGTCATACTCAACTAATTGG+GGG - chr7_1:2308108-2308127 Msa1004710:intron 35.0%
! ATAGGCCATCAGAAATTTTG+TGG - chr7_1:2308283-2308302 Msa1004710:intron 35.0%
CCTTTCACAGAGACAATATT+AGG + chr7_1:2308583-2308602 None:intergenic 35.0%
CCTAATATTGTCTCTGTGAA+AGG - chr7_1:2308580-2308599 Msa1004710:intron 35.0%
TTGGTTTCTCATCCATAGTT+GGG - chr7_1:2309241-2309260 Msa1004710:intron 35.0%
CATGTAAGAAATCCCAACTA+TGG + chr7_1:2309256-2309275 None:intergenic 35.0%
CCGAATTATATCATTGGGAA+AGG - chr7_1:2309324-2309343 Msa1004710:intron 35.0%
CTATTCCAGTGAAGCTATAA+AGG + chr7_1:2309405-2309424 None:intergenic 35.0%
GCAATAACTTAAACTGGCAA+TGG + chr7_1:2309442-2309461 None:intergenic 35.0%
!! CCTTTAAAGTGAAATGTTGG+TGG + chr7_1:2309613-2309632 None:intergenic 35.0%
CCACCAACATTTCACTTTAA+AGG - chr7_1:2309610-2309629 Msa1004710:intron 35.0%
ATGCCTTAAATGTGAAGTGT+CGG + chr7_1:2309684-2309703 None:intergenic 35.0%
! ACTTCACATTTAAGGCATGT+CGG - chr7_1:2309686-2309705 Msa1004710:intron 35.0%
!! ATAAAGCACTGTCAAAGATC+TGG + chr7_1:2309774-2309793 None:intergenic 35.0%
AGATCAGGGAAGATAAAAAG+AGG + chr7_1:2309868-2309887 None:intergenic 35.0%
! AGTTCTTCAGGTTAGTATTC+AGG - chr7_1:2309910-2309929 Msa1004710:intron 35.0%
TTAATGATATAGGATGGCAC+AGG + chr7_1:2310044-2310063 None:intergenic 35.0%
TTTCTTCTTCCTACCATTGT+CGG - chr7_1:2310104-2310123 Msa1004710:intron 35.0%
!!! TCTTACTTTTTCCCCATAGT+TGG - chr7_1:2310221-2310240 Msa1004710:CDS 35.0%
ATATGATCGGAGTAGTAAGG+CGG + chr7_1:2305013-2305032 None:intergenic 40.0%
TTACTACTCCGATCATATGG+CGG - chr7_1:2305015-2305034 Msa1004710:three_prime_UTR 40.0%
GCTTCCTTATCTTTCTCCAT+TGG - chr7_1:2305264-2305283 Msa1004710:CDS 40.0%
CGTACAATACACCAAATGGT+GGG + chr7_1:2305556-2305575 None:intergenic 40.0%
GATGTTGTCATGTGACTGTA+AGG + chr7_1:2305632-2305651 None:intergenic 40.0%
GGAGGCAGAATCAATTCTAT+CGG - chr7_1:2306534-2306553 Msa1004710:intron 40.0%
TAGTGTTAGAACAGCAAAGC+TGG - chr7_1:2307834-2307853 Msa1004710:intron 40.0%
TAATAGGTCAAACCCTAGAG+CGG + chr7_1:2308070-2308089 None:intergenic 40.0%
ATACTCAACTAATTGGGGGA+TGG - chr7_1:2308112-2308131 Msa1004710:intron 40.0%
ACTATGGATGAGAAACCAAG+AGG + chr7_1:2309240-2309259 None:intergenic 40.0%
CTTGGTTTCTCATCCATAGT+TGG - chr7_1:2309240-2309259 Msa1004710:intron 40.0%
CATGGACTCAACATGCTTAA+AGG + chr7_1:2309381-2309400 None:intergenic 40.0%
!! AAAGTGAAATGTTGGTGGCT+CGG + chr7_1:2309608-2309627 None:intergenic 40.0%
! GGAAGTTTGTTCTTCTTGCT+CGG - chr7_1:2310012-2310031 Msa1004710:intron 40.0%
ACATCTATAGGAAGTCTTGC+TGG - chr7_1:2310152-2310171 Msa1004710:intron 40.0%
TTGAGATTTCTTAGAGCCAG+AGG + chr7_1:2310195-2310214 None:intergenic 40.0%
TTCGTCCCAAATTTCAGTAC+CGG - chr7_1:2310286-2310305 Msa1004710:CDS 40.0%
! TCAATAACATTATGGCGGGG+TGG - chr7_1:2304956-2304975 Msa1004710:three_prime_UTR 45.0%
GCCTTACTACTCCGATCATA+TGG - chr7_1:2305012-2305031 Msa1004710:three_prime_UTR 45.0%
GCCATATGATCGGAGTAGTA+AGG + chr7_1:2305016-2305035 None:intergenic 45.0%
TTGCTTCTCCGCCATATGAT+CGG + chr7_1:2305026-2305045 None:intergenic 45.0%
! TCATATGGCGGAGAAGCAAT+CGG - chr7_1:2305027-2305046 Msa1004710:three_prime_UTR 45.0%
ACTGGTGCTTCTCTTCTCTT+TGG - chr7_1:2305195-2305214 Msa1004710:CDS 45.0%
GTGCTTCTCTTCTCTTTGGT+TGG - chr7_1:2305199-2305218 Msa1004710:CDS 45.0%
AGAAAGATAAGGAAGCAGCG+AGG + chr7_1:2305260-2305279 None:intergenic 45.0%
CCTGCCAATGGAGAAAGATA+AGG + chr7_1:2305271-2305290 None:intergenic 45.0%
CCTTATCTTTCTCCATTGGC+AGG - chr7_1:2305268-2305287 Msa1004710:CDS 45.0%
GCGTACAATACACCAAATGG+TGG + chr7_1:2305557-2305576 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG + chr7_1:2305560-2305579 None:intergenic 45.0%
CGGCCTCTTGTGTAAAAACA+GGG + chr7_1:2305587-2305606 None:intergenic 45.0%
ACGGCCTCTTGTGTAAAAAC+AGG + chr7_1:2305588-2305607 None:intergenic 45.0%
ACGGGTTTAAGTCCTGGAAA+CGG + chr7_1:2305607-2305626 None:intergenic 45.0%
TCATGTGACTGTAAGGTCAC+GGG + chr7_1:2305625-2305644 None:intergenic 45.0%
GTCATGTGACTGTAAGGTCA+CGG + chr7_1:2305626-2305645 None:intergenic 45.0%
CATCTTTACCAGTTGCGCTA+AGG - chr7_1:2305648-2305667 Msa1004710:intron 45.0%
CATCTTTACCAGTTGCGCTA+AGG - chr7_1:2305648-2305667 Msa1004710:intron 45.0%
!! TTAGCAGGTTTAGGGGTGTT+TGG - chr7_1:2307686-2307705 Msa1004710:intron 45.0%
GACGGAGACGTAAGCATAAT+TGG + chr7_1:2307918-2307937 None:intergenic 45.0%
TTACGTCTCCGTCGATAGAT+TGG - chr7_1:2307925-2307944 Msa1004710:intron 45.0%
CTCAGTAGCCAATCTATCGA+CGG + chr7_1:2307936-2307955 None:intergenic 45.0%
CTAAAAGTAGGCAGACGGTT+CGG + chr7_1:2308036-2308055 None:intergenic 45.0%
TGGAGCTAAAAGTAGGCAGA+CGG + chr7_1:2308041-2308060 None:intergenic 45.0%
GAGCAAGTGGAGCTAAAAGT+AGG + chr7_1:2308048-2308067 None:intergenic 45.0%
AAAGGACTGCTGGGAACATT+AGG - chr7_1:2308598-2308617 Msa1004710:intron 45.0%
! TCTATGGTAATGAAGGGTGC+TGG - chr7_1:2308683-2308702 Msa1004710:intron 45.0%
CAGTGAAGCTATAAAGGGCA+TGG + chr7_1:2309399-2309418 None:intergenic 45.0%
CATGCCCTTTATAGCTTCAC+TGG - chr7_1:2309397-2309416 Msa1004710:intron 45.0%
!! GTCGGTGCTACAGGATAAAT+TGG + chr7_1:2309666-2309685 None:intergenic 45.0%
! GCACCGACACTTCACATTTA+AGG - chr7_1:2309678-2309697 Msa1004710:intron 45.0%
CAGGAATTCAACCTCAGCAA+TGG - chr7_1:2309929-2309948 Msa1004710:intron 45.0%
ATTCAACCTCAGCAATGGAC+TGG - chr7_1:2309934-2309953 Msa1004710:intron 45.0%
! TTCAACCTCAGCAATGGACT+GGG - chr7_1:2309935-2309954 Msa1004710:intron 45.0%
CCTAACTTCAGTAGCAGCTA+AGG + chr7_1:2310072-2310091 None:intergenic 45.0%
CCTTAGCTGCTACTGAAGTT+AGG - chr7_1:2310069-2310088 Msa1004710:intron 45.0%
TCTGTTAGTAAACTGGTGCG+TGG - chr7_1:2310391-2310410 Msa1004710:CDS 45.0%
TCGATCGGTAAACTGAAGGA+CGG + chr7_1:2310431-2310450 None:intergenic 45.0%
CAGTTCGATCGGTAAACTGA+AGG + chr7_1:2310435-2310454 None:intergenic 45.0%
TACCGATCGAACTGTCTGTT+TGG - chr7_1:2310441-2310460 Msa1004710:CDS 45.0%
ACCGATCGAACTGTCTGTTT+GGG - chr7_1:2310442-2310461 Msa1004710:CDS 45.0%
TCCCAAACAGACAGTTCGAT+CGG + chr7_1:2310446-2310465 None:intergenic 45.0%
! ATAACATTATGGCGGGGTGG+TGG - chr7_1:2304959-2304978 Msa1004710:three_prime_UTR 50.0%
GAAGCAATCGGAATCGGAGT+CGG - chr7_1:2305039-2305058 Msa1004710:three_prime_UTR 50.0%
TTACACAAGAGGCCGTTTCC+AGG - chr7_1:2305592-2305611 Msa1004710:intron 50.0%
AAGGTCACGGGTTTAAGTCC+TGG + chr7_1:2305613-2305632 None:intergenic 50.0%
AGAGGTAACCTTAGCGCAAC+TGG + chr7_1:2305659-2305678 None:intergenic 50.0%
AGAGGTAACCTTAGCGCAAC+TGG + chr7_1:2305659-2305678 None:intergenic 50.0%
CTTATTCCCTCTGCTTCCTC+TGG + chr7_1:2308485-2308504 None:intergenic 50.0%
TGTCTCTGTGAAAGGACTGC+TGG - chr7_1:2308588-2308607 Msa1004710:intron 50.0%
GTCTCTGTGAAAGGACTGCT+GGG - chr7_1:2308589-2308608 Msa1004710:intron 50.0%
!! ATGTGAAGTGTCGGTGCTAC+AGG + chr7_1:2309675-2309694 None:intergenic 50.0%
CTCGCTCAACTCCATTTGCA+AGG + chr7_1:2309986-2310005 None:intergenic 50.0%
GCAAATGGAGTTGAGCGAGA+TGG - chr7_1:2309987-2310006 Msa1004710:intron 50.0%
ATGGAGTTGAGCGAGATGGT+TGG - chr7_1:2309991-2310010 Msa1004710:intron 50.0%
GGCGGAGAAGCAATCGGAAT+CGG - chr7_1:2305033-2305052 Msa1004710:three_prime_UTR 55.0%
CGACGTGTTCGTGTCTGTGT+CGG + chr7_1:2305361-2305380 None:intergenic 55.0%
ACTGAAGCTCCCGCATACAC+AGG - chr7_1:2305516-2305535 Msa1004710:intron 55.0%
CTGAAGCTCCCGCATACACA+GGG - chr7_1:2305517-2305536 Msa1004710:intron 55.0%
TTACCGGACCCTGTGTATGC+GGG + chr7_1:2305528-2305547 None:intergenic 55.0%
CTTACCGGACCCTGTGTATG+CGG + chr7_1:2305529-2305548 None:intergenic 55.0%
GCATACACAGGGTCCGGTAA+GGG - chr7_1:2305528-2305547 Msa1004710:intron 55.0%
CATACACAGGGTCCGGTAAG+GGG - chr7_1:2305529-2305548 Msa1004710:intron 55.0%
! CAAATGGTGGGACCCCTTAC+CGG + chr7_1:2305544-2305563 None:intergenic 55.0%
! AAAGTAGGCAGACGGTTCGG+CGG + chr7_1:2308033-2308052 None:intergenic 55.0%
AAACCCTAGAGCGGAGCAAG+TGG + chr7_1:2308061-2308080 None:intergenic 55.0%
! ACTGGCTTCTCCTGTACCAG+AGG - chr7_1:2308466-2308485 Msa1004710:intron 55.0%
TCCTGTACCAGAGGAAGCAG+AGG - chr7_1:2308475-2308494 Msa1004710:intron 55.0%
CCTGTACCAGAGGAAGCAGA+GGG - chr7_1:2308476-2308495 Msa1004710:intron 55.0%
TCTAGCCCAGTCCATTGCTG+AGG + chr7_1:2309943-2309962 None:intergenic 55.0%
!! CAGCAATGGACTGGGCTAGA+AGG - chr7_1:2309943-2309962 Msa1004710:intron 55.0%
GGGATGCATCCGACAATGGT+AGG + chr7_1:2310116-2310135 None:intergenic 55.0%
CATGGGGATGCATCCGACAA+TGG + chr7_1:2310120-2310139 None:intergenic 55.0%
! ACATTATGGCGGGGTGGTGG+TGG - chr7_1:2304962-2304981 Msa1004710:three_prime_UTR 60.0%
!! GGGGTGGTGGTGGAAAACAG+CGG - chr7_1:2304972-2304991 Msa1004710:three_prime_UTR 60.0%
ATGCGGGAGCTTCAGTGCAC+CGG + chr7_1:2305512-2305531 None:intergenic 60.0%
CGCATACACAGGGTCCGGTA+AGG - chr7_1:2305527-2305546 Msa1004710:intron 60.0%
CGGTAAGGGGTCCCACCATT+TGG - chr7_1:2305542-2305561 Msa1004710:intron 60.0%
AGCTCCACTTGCTCCGCTCT+AGG - chr7_1:2308054-2308073 Msa1004710:intron 60.0%
GCTCCACTTGCTCCGCTCTA+GGG - chr7_1:2308055-2308074 Msa1004710:intron 60.0%
CCCTCTGCTTCCTCTGGTAC+AGG + chr7_1:2308479-2308498 None:intergenic 60.0%
GCAGCTGTTGCTACAGCCTC+TGG - chr7_1:2310176-2310195 Msa1004710:CDS 60.0%
TGCGGGAGCTTCAGTGCACC+GGG + chr7_1:2305511-2305530 None:intergenic 65.0%
GCTCCCGCATACACAGGGTC+CGG - chr7_1:2305522-2305541 Msa1004710:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 2304857 2310593 2304857 ID=Msa1004710;Name=Msa1004710
chr7_1 mRNA 2304857 2310593 2304857 ID=Msa1004710-mRNA-1;Parent=Msa1004710;Name=Msa1004710-mRNA-1;_AED=0.01;_eAED=0.01;_QI=110|1|1|1|1|1|2|235|253
chr7_1 exon 2304857 2305530 2304857 ID=Msa1004710-mRNA-1:exon:908;Parent=Msa1004710-mRNA-1
chr7_1 exon 2310161 2310593 2310161 ID=Msa1004710-mRNA-1:exon:907;Parent=Msa1004710-mRNA-1
chr7_1 five_prime_UTR 2310484 2310593 2310484 ID=Msa1004710-mRNA-1:five_prime_utr;Parent=Msa1004710-mRNA-1
chr7_1 CDS 2310161 2310483 2310161 ID=Msa1004710-mRNA-1:cds;Parent=Msa1004710-mRNA-1
chr7_1 CDS 2305092 2305530 2305092 ID=Msa1004710-mRNA-1:cds;Parent=Msa1004710-mRNA-1
chr7_1 three_prime_UTR 2304857 2305091 2304857 ID=Msa1004710-mRNA-1:three_prime_utr;Parent=Msa1004710-mRNA-1
Gene Sequence

>Msa1004710

ATGGCGGGGTGGTGGTGGAAAACAGCGGTGTTAATAGCAATATCCGCCTTACTACTCCGATCATATGGCGGAGAAGCAATCGGAATCGGAGTCGGAATCGATAAAGAATCATTGATGAAATGGATGAGAGATTTATCTGATAAACTAGGAATTTGGGCGATTCCACTATACATCGCTATTCATACAATTTCAATTGCTCTTTGTTTACCTTCTGCTATTTTTCTTGAAACTGGTGCTTCTCTTCTCTTTGGTTGGTTTGCTTCTGTTCTTTGTGTTTTCTCTGCTAAGATCCTCGCTGCTTCCTTATCTTTCTCCATTGGCAGGTTAGTATTCAGGAATTCAACCTCAGCAATGGACTGGGCTAGAAGGAACAAATATTTTAAAATCCTTGCAAATGGAGTTGAGCGAGATGGTTGGAAGTTTGTTCTTCTTGCTCGGTTCTCGCCTGTGCCATCCTATATCATTAACTATACCTTAGCTGCTACTGAAGTTAGGTTCTTTTTGGATTTTCTTCTTCCTACCATTGTCGGATGCATCCCCATGATCTTACAGAATACATCTATAGGAAGTCTTGCTGGTGCAGCTGTTGCTACAGCCTCTGGCTCTAAGAAATCTCAATTTTGGTCTTACTTTTTCCCCATAGTTGGTATTTTATCTAGTGTACTTATTTCTTTGAGAATTAAAAAGTATTCGTCCCAAATTTCAGTACCGGAAATTTCTTCTGACAAAGATAACACTGTTGAGTCAAAACAACAAAAATAA
Protein sequence

>Msa1004710

MAGWWWKTAVLIAISALLLRSYGGEAIGIGVGIDKESLMKWMRDLSDKLGIWAIPLYIAIHTISIALCLPSAIFLETGASLLFGWFASVLCVFSAKILAASLSFSIGRLVFRNSTSAMDWARRNKYFKILANGVERDGWKFVLLARFSPVPSYIINYTLAATEVRFFLDFLLPTIVGCIPMILQNTSIGSLAGAAVATASGSKKSQFWSYFFPIVGILSSVLISLRIKKYSSQISVPEISSDKDNTVESKQQK*