AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1091220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1091220 MtrunA17_Chr7g0214791 96.651 209 7 0 1 209 1 209 7.52e-145 409
Msa1091220 MtrunA17_Chr7g0214791 100.000 145 0 0 107 251 207 351 3.90e-99 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1091220 AT1G44960.1 67.299 211 67 1 30 240 26 234 2.34e-103 301

Find 71 sgRNAs with CRISPR-Local

Find 295 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTCCGGTACTGAAATT+TGG 0.132573 7_3:+6529395 None:intergenic
TATCTGATAAACTAGGAATT+TGG 0.145494 7_3:-6534590 Msa1091220:CDS
GCTCTAAGAAATCTCAATTT+TGG 0.229017 7_3:-6529489 Msa1091220:CDS
AGTTCTTCAGGTTAGTATTC+AGG 0.245543 7_3:-6529777 Msa1091220:intron
ACTGGTGCTTCTATTCTCTT+TGG 0.281656 7_3:-6534495 Msa1091220:CDS
ACTAGATAAAATACCAACTA+TGG 0.282343 7_3:+6529453 None:intergenic
AATACTAACAACAACAAAAT+TGG 0.287741 7_3:+6529188 None:intergenic
ATCTGATAAACTAGGAATTT+GGG 0.313710 7_3:-6534589 Msa1091220:CDS
GACTTGATCTTCATTTAATC+TGG 0.331073 7_3:-6529116 Msa1091220:three_prime_UTR
AATTTCCGGTACTGAAATTT+GGG 0.342524 7_3:+6529396 None:intergenic
CCTTAGCTGCTACTGAAGTT+AGG 0.357035 7_3:-6529618 Msa1091220:CDS
GCAGCTGTTGCTACAGCCTC+TGG 0.372165 7_3:-6529511 Msa1091220:CDS
ATAAAGAATCATTGATGAAA+TGG 0.374496 7_3:-6534623 Msa1091220:CDS
TACCGATCGAACTGTCTGTT+TGG 0.377737 7_3:-6529246 Msa1091220:three_prime_UTR
TCTTTGTCAGAAGAAATTTC+CGG 0.385161 7_3:+6529382 None:intergenic
CAGCAATGGACTGGGCTAGA+AGG 0.407462 7_3:-6529744 Msa1091220:CDS
ACCGATCGAACTGTCTGTTT+GGG 0.409473 7_3:-6529245 Msa1091220:three_prime_UTR
GAATAGAAGCACCAGTTTCA+AGG 0.425167 7_3:+6534502 None:intergenic
CCTTATCTTTCTCCATTGGC+AGG 0.426737 7_3:-6534422 Msa1091220:intron
TTTCTTCTTCCTACCATTGT+CGG 0.428961 7_3:-6529583 Msa1091220:CDS
GAATTAAATCAATAACATAA+TGG 0.432097 7_3:-6534736 Msa1091220:exon
GGAAGTTTGTTCTTCTTGCT+CGG 0.432228 7_3:-6529675 Msa1091220:CDS
GTGCTTCTATTCTCTTTGGT+TGG 0.434613 7_3:-6534491 Msa1091220:CDS
GGCGGAGAAGCAATCGGAAT+CGG 0.440129 7_3:-6534651 Msa1091220:CDS
ACATCTATAGGAAGTCTTGC+TGG 0.441068 7_3:-6529535 Msa1091220:CDS
TAAGGTATAGTTAATGATAT+AGG 0.443398 7_3:+6529636 None:intergenic
ACATAATGGCGGGGTGGTGG+TGG 0.456256 7_3:-6534722 Msa1091220:exon
GCCTTACTACTCCGATCATA+TGG 0.458022 7_3:-6534672 Msa1091220:CDS
TAAATCAATAACATAATGGC+GGG 0.458116 7_3:-6534732 Msa1091220:exon
GCTTCCTTATCTTTCTCCAT+TGG 0.464011 7_3:-6534426 Msa1091220:CDS
ATCTTACAGAATACATCTAT+AGG 0.469430 7_3:-6529547 Msa1091220:CDS
AGATGTATTCTGTAAGATCA+TGG 0.472448 7_3:+6529552 None:intergenic
TTCGTCCCAAATTTCAGTAC+CGG 0.474329 7_3:-6529401 Msa1091220:CDS
ATAACATAATGGCGGGGTGG+TGG 0.489266 7_3:-6534725 Msa1091220:exon
ATTCAACCTCAGCAATGGAC+TGG 0.500375 7_3:-6529753 Msa1091220:CDS
TCATATGGCGGAGAAGCAAT+CGG 0.500801 7_3:-6534657 Msa1091220:CDS
TTCAACCTCAGCAATGGACT+GGG 0.502524 7_3:-6529752 Msa1091220:CDS
GCCATATGATCGGAGTAGTA+AGG 0.504927 7_3:+6534671 None:intergenic
CCTAACTTCAGTAGCAGCTA+AGG 0.506076 7_3:+6529618 None:intergenic
GATGTATTCTGTAAGATCAT+GGG 0.518629 7_3:+6529553 None:intergenic
CTAGATAAAATACCAACTAT+GGG 0.519361 7_3:+6529454 None:intergenic
CCTGCCAATGGAGAAAGATA+AGG 0.520262 7_3:+6534422 None:intergenic
TTGCTTCTCCGCCATATGAT+CGG 0.526914 7_3:+6534661 None:intergenic
CTCGCTCAACTCCATTTGCA+AGG 0.541452 7_3:+6529704 None:intergenic
AAAATTATCTAGTAGTGAAG+AGG 0.553803 7_3:-6529330 Msa1091220:three_prime_UTR
AATCATATGTTACCTGCCAA+TGG 0.556218 7_3:+6534410 None:intergenic
AGAGATTTATCTGATAAACT+AGG 0.558993 7_3:-6534597 Msa1091220:CDS
GTGTGAATAGCGATGTAAAG+TGG 0.559311 7_3:+6534561 None:intergenic
GGGATGCATCCGACAATGGT+AGG 0.579071 7_3:+6529574 None:intergenic
TTGAGATTTCTTAGAGCCAG+AGG 0.582555 7_3:+6529495 None:intergenic
TCTAGCCCAGTCCATTGCTG+AGG 0.592500 7_3:+6529747 None:intergenic
ATGGAGTTGAGCGAGATGGT+TGG 0.593190 7_3:-6529696 Msa1091220:CDS
CAGGAATTCAACCTCAGCAA+TGG 0.593899 7_3:-6529758 Msa1091220:CDS
TCCCAAACAGACAGTTCGAT+CGG 0.605038 7_3:+6529244 None:intergenic
CATGGGGATGCATCCGACAA+TGG 0.608315 7_3:+6529570 None:intergenic
ATGTATTCTGTAAGATCATG+GGG 0.610828 7_3:+6529554 None:intergenic
TTAAATCAATAACATAATGG+CGG 0.617001 7_3:-6534733 Msa1091220:exon
TCTGTTAGTAAACTGGTGCG+TGG 0.633463 7_3:-6529296 Msa1091220:three_prime_UTR
GTATAGTTAATGATATAGGA+TGG 0.638706 7_3:+6529640 None:intergenic
GTTTCAAGGAAAATAGCAGA+AGG 0.643085 7_3:+6534516 None:intergenic
GCAAATGGAGTTGAGCGAGA+TGG 0.646060 7_3:-6529700 Msa1091220:CDS
CAGTTCGATCGGTAAACTGA+AGG 0.647256 7_3:+6529255 None:intergenic
AAATCAATAACATAATGGCG+GGG 0.648875 7_3:-6534731 Msa1091220:exon
TTAATGATATAGGATGGCAC+AGG 0.650154 7_3:+6529646 None:intergenic
GGGGTGGTGGTGGAAAACAG+CGG 0.679901 7_3:-6534712 Msa1091220:CDS
TAGATAAAATACCAACTATG+GGG 0.680141 7_3:+6529455 None:intergenic
TCAATAACATAATGGCGGGG+TGG 0.686065 7_3:-6534728 Msa1091220:exon
TCGATCGGTAAACTGAAGGA+CGG 0.705675 7_3:+6529259 None:intergenic
TTACTACTCCGATCATATGG+CGG 0.710739 7_3:-6534669 Msa1091220:CDS
ATATGATCGGAGTAGTAAGG+CGG 0.729764 7_3:+6534674 None:intergenic
AGAAAGATAAGGAAGCAGCG+AGG 0.748647 7_3:+6534433 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAGTTGATTTAATAATTA+AGG + chr7_3:6531224-6531243 None:intergenic 10.0%
!!! TTAATTTATTATGTTGTTTT+AGG - chr7_3:6531242-6531261 Msa1091220:intron 10.0%
!! TTAATTCATTAAATTGTTAT+AGG - chr7_3:6531913-6531932 Msa1091220:intron 10.0%
!! TAGTCATTAATATTAATAAT+AGG + chr7_3:6532346-6532365 None:intergenic 10.0%
!! AAATAAACATGTTTAAATTT+AGG - chr7_3:6533178-6533197 Msa1091220:intron 10.0%
!!! AATTTTTCAACAAAATTTTA+AGG + chr7_3:6533278-6533297 None:intergenic 10.0%
!! GAATTAAATCAATAACATAA+TGG - chr7_3:6529213-6529232 Msa1091220:three_prime_UTR 15.0%
!!! TTTCTTTTTCACATTTTTAT+CGG + chr7_3:6529646-6529665 None:intergenic 15.0%
!! TAGAAACTTAGAATTAAAAA+AGG - chr7_3:6529740-6529759 Msa1091220:CDS 15.0%
!! AGAAACTTAGAATTAAAAAA+GGG - chr7_3:6529741-6529760 Msa1091220:CDS 15.0%
!!! TAGAATTAGTTGAATAATTT+TGG - chr7_3:6529951-6529970 Msa1091220:intron 15.0%
!!! TAGAATTAGTTGAATAATTT+TGG - chr7_3:6529951-6529970 Msa1091220:intron 15.0%
!!! TGCATTTAATTAATTTTTGT+AGG - chr7_3:6530128-6530147 Msa1091220:intron 15.0%
!!! GCATTTAATTAATTTTTGTA+GGG - chr7_3:6530370-6530389 Msa1091220:intron 15.0%
!!! CATTTAATTAATTTTTGTAG+GGG - chr7_3:6530371-6530390 Msa1091220:intron 15.0%
!! TATATTATTAAATGTGTGTT+TGG - chr7_3:6530593-6530612 Msa1091220:intron 15.0%
!!! TGATTTTGTAAATTTGATTT+TGG - chr7_3:6530648-6530667 Msa1091220:intron 15.0%
!!! GAATAAATTTGAGTTAAATA+TGG - chr7_3:6530721-6530740 Msa1091220:intron 15.0%
!!! AATTTTTGTTTTTTCAAAAG+TGG - chr7_3:6530860-6530879 Msa1091220:intron 15.0%
!! TTAAATAAACTAATTGTGTT+TGG - chr7_3:6531328-6531347 Msa1091220:intron 15.0%
!!! TTTTGTAGTTTTTTAAATTG+TGG - chr7_3:6531492-6531511 Msa1091220:intron 15.0%
!! AAAAAAATACAAAGAATTTG+AGG - chr7_3:6532056-6532075 Msa1091220:intron 15.0%
!! TTAAATCAATAACATAATGG+CGG - chr7_3:6529216-6529235 Msa1091220:three_prime_UTR 20.0%
!! ATAAAGAATCATTGATGAAA+TGG - chr7_3:6529326-6529345 Msa1091220:three_prime_UTR 20.0%
!!! TAGTTGAATAATTTTGGTTA+AGG - chr7_3:6529957-6529976 Msa1091220:intron 20.0%
!!! TAGTTGAATAATTTTGGTTA+AGG - chr7_3:6529957-6529976 Msa1091220:intron 20.0%
!!! GGTTTAAGTTTAATCAATTA+TGG - chr7_3:6530772-6530791 Msa1091220:intron 20.0%
!! TTAAGTTTAATCAATTATGG+AGG - chr7_3:6530775-6530794 Msa1091220:intron 20.0%
!!! AAAACAAAAATTGATTCTAG+AGG + chr7_3:6530852-6530871 None:intergenic 20.0%
!! TAATTGGATGTAAATTAGTT+TGG - chr7_3:6531066-6531085 Msa1091220:intron 20.0%
!! ATGATATGATGAATTCTAAT+TGG - chr7_3:6531114-6531133 Msa1091220:intron 20.0%
!! AAATTAACAACTAAAATGCT+AGG + chr7_3:6531620-6531639 None:intergenic 20.0%
!! AAACTATCAAATAAAACTCT+TGG + chr7_3:6531873-6531892 None:intergenic 20.0%
!! TTAAATTGTTATAGGAAGTT+AGG - chr7_3:6531921-6531940 Msa1091220:intron 20.0%
!! CTTAATAAACTATTTGTGTT+TGG - chr7_3:6532005-6532024 Msa1091220:intron 20.0%
!! CATACATGTTTATATCATTA+AGG - chr7_3:6532490-6532509 Msa1091220:intron 20.0%
!! AATCTTTGTAATCAAATGAT+TGG - chr7_3:6532680-6532699 Msa1091220:intron 20.0%
!!! TTGTTTTTTTTTGTTATGCT+AGG - chr7_3:6532894-6532913 Msa1091220:intron 20.0%
!!! AAATATTCTATGGTAATGAA+GGG - chr7_3:6532938-6532957 Msa1091220:intron 20.0%
!!! GCTTATTTTTGTTTTGTATA+CGG - chr7_3:6532982-6533001 Msa1091220:intron 20.0%
!! TGAGTTAAAATTGATAGAAT+AGG - chr7_3:6533086-6533105 Msa1091220:intron 20.0%
!! TGTTGAAAAATTTGATAAGT+TGG - chr7_3:6533286-6533305 Msa1091220:intron 20.0%
!!! CTTCTAAAATAAAAAATCAC+AGG + chr7_3:6533393-6533412 None:intergenic 20.0%
!! TTTATAAAAATGTATCCTCT+TGG - chr7_3:6533484-6533503 Msa1091220:intron 20.0%
!! AATTATAAGTGGTAGTTTAT+AGG - chr7_3:6533770-6533789 Msa1091220:intron 20.0%
!! ATTTGAGAAAATGGATAAAT+TGG + chr7_3:6533996-6534015 None:intergenic 20.0%
!! GTGATAAATATTTGAGAAAA+TGG + chr7_3:6534005-6534024 None:intergenic 20.0%
!! GACAATACATTATTAACATA+TGG + chr7_3:6534081-6534100 None:intergenic 20.0%
!!! TATTTTAAAATCCTTGCAAA+TGG - chr7_3:6534234-6534253 Msa1091220:intron 20.0%
!! TAAGGTATAGTTAATGATAT+AGG + chr7_3:6534316-6534335 None:intergenic 20.0%
!! AATACTAACAACAACAAAAT+TGG + chr7_3:6534764-6534783 None:intergenic 20.0%
!! AAGAGTATAGTCAACAAAAA+CGG - chr7_3:6529135-6529154 Msa1091220:three_prime_UTR 25.0%
! TAAATCAATAACATAATGGC+GGG - chr7_3:6529217-6529236 Msa1091220:three_prime_UTR 25.0%
! AGAGATTTATCTGATAAACT+AGG - chr7_3:6529352-6529371 Msa1091220:CDS 25.0%
! TATCTGATAAACTAGGAATT+TGG - chr7_3:6529359-6529378 Msa1091220:CDS 25.0%
! ATCTGATAAACTAGGAATTT+GGG - chr7_3:6529360-6529379 Msa1091220:CDS 25.0%
!!! GTTTTTGGTAAAAAAAGTAG+CGG - chr7_3:6529992-6530011 Msa1091220:intron 25.0%
! ACTGATAAGATAGTTTATAG+TGG - chr7_3:6530018-6530037 Msa1091220:intron 25.0%
! ACTTATAAGCTAACTTATAG+CGG - chr7_3:6530044-6530063 Msa1091220:intron 25.0%
!!! GTTTTTGGTAAAAAAAGTAG+CGG - chr7_3:6529992-6530011 Msa1091220:intron 25.0%
! ACTGATAAGATAGTTTATAG+TGG - chr7_3:6530018-6530037 Msa1091220:intron 25.0%
! ACTTATAAGCTAACTTATAG+CGG - chr7_3:6530044-6530063 Msa1091220:intron 25.0%
!! TAATTTTTGTAGGGGTAAAA+TGG - chr7_3:6530379-6530398 Msa1091220:intron 25.0%
!! TTATTTTCATGAGCTTATAG+AGG + chr7_3:6530453-6530472 None:intergenic 25.0%
! AAGACATAAAATTGACCTTA+TGG + chr7_3:6530551-6530570 None:intergenic 25.0%
! CGATAAATTTAGTGTATGTT+TGG + chr7_3:6530888-6530907 None:intergenic 25.0%
!!! ATTTTTTGTCGATGAGAAAA+AGG - chr7_3:6530944-6530963 Msa1091220:intron 25.0%
!!! TGTAGTTATTTGTTATGTTG+TGG - chr7_3:6531007-6531026 Msa1091220:intron 25.0%
! AAATTGACAACTAAAACACT+AGG + chr7_3:6531200-6531219 None:intergenic 25.0%
!!! TTATGTTGTTTTAGGAAGTA+AGG - chr7_3:6531250-6531269 Msa1091220:intron 25.0%
! GTTTATTAAGTTAAAGAGCT+TGG + chr7_3:6531995-6532014 None:intergenic 25.0%
! AAAATACAAAGAATTTGAGG+TGG - chr7_3:6532059-6532078 Msa1091220:intron 25.0%
! TGTACTATTATGTAATGCTT+GGG - chr7_3:6532216-6532235 Msa1091220:intron 25.0%
!!! TCTTTTGTATGTCGATTTAT+AGG - chr7_3:6532526-6532545 Msa1091220:intron 25.0%
!! AAATTTTGTGGTTTCTTTGT+TGG - chr7_3:6532556-6532575 Msa1091220:intron 25.0%
! TGAAGCTTATATTAAAAGCA+GGG - chr7_3:6532588-6532607 Msa1091220:intron 25.0%
! TCATTTGATTACAAAGATTG+AGG + chr7_3:6532679-6532698 None:intergenic 25.0%
! TTTGTAATCAAATGATTGGT+TGG - chr7_3:6532684-6532703 Msa1091220:intron 25.0%
!! TAATTTGAGATGAAAGTTAC+TGG - chr7_3:6532709-6532728 Msa1091220:intron 25.0%
! TAGAATATTTGCACAGAAAA+AGG + chr7_3:6532928-6532947 None:intergenic 25.0%
!! TTTTCTGTGCAAATATTCTA+TGG - chr7_3:6532928-6532947 Msa1091220:intron 25.0%
!! CAAATATTCTATGGTAATGA+AGG - chr7_3:6532937-6532956 Msa1091220:intron 25.0%
!! AGTTTTGCATCTTCATTTTA+CGG - chr7_3:6533118-6533137 Msa1091220:intron 25.0%
!!! AAATTTGATAAGTTGGTTGA+AGG - chr7_3:6533293-6533312 Msa1091220:intron 25.0%
! CTATTCCGAATTATATCATT+GGG - chr7_3:6533581-6533600 Msa1091220:intron 25.0%
! ATATATTGTCTGATTCAAGT+TGG - chr7_3:6533617-6533636 Msa1091220:intron 25.0%
! AAAGAAGCAATAACTTAAAC+TGG + chr7_3:6533710-6533729 None:intergenic 25.0%
! GTCATTGTATGAATTATAAG+TGG - chr7_3:6533759-6533778 Msa1091220:intron 25.0%
! TTGGATGTAATAATATGTGT+TGG + chr7_3:6533909-6533928 None:intergenic 25.0%
! TTGGATGTAATAATATGTGT+CGG + chr7_3:6533909-6533928 None:intergenic 25.0%
! TTTATTAGTAACAGCTAACA+AGG - chr7_3:6534051-6534070 Msa1091220:intron 25.0%
! GTATAGTTAATGATATAGGA+TGG + chr7_3:6534312-6534331 None:intergenic 25.0%
! ATCTTACAGAATACATCTAT+AGG - chr7_3:6534402-6534421 Msa1091220:intron 25.0%
! TAGATAAAATACCAACTATG+GGG + chr7_3:6534497-6534516 None:intergenic 25.0%
! CTAGATAAAATACCAACTAT+GGG + chr7_3:6534498-6534517 None:intergenic 25.0%
! ACTAGATAAAATACCAACTA+TGG + chr7_3:6534499-6534518 None:intergenic 25.0%
! AAAATTATCTAGTAGTGAAG+AGG - chr7_3:6534619-6534638 Msa1091220:CDS 25.0%
!! ATGTTTTTCTGTTAGTAAAC+TGG - chr7_3:6534646-6534665 Msa1091220:CDS 25.0%
AAATCAATAACATAATGGCG+GGG - chr7_3:6529218-6529237 Msa1091220:three_prime_UTR 30.0%
TTCTAAGTTTCTAACAAGAG+AGG + chr7_3:6529936-6529955 None:intergenic 30.0%
!!! AGGTAGTTGTGATTAGTTTT+TGG - chr7_3:6529977-6529996 Msa1091220:intron 30.0%
TTCTAAGTTTCTAACAAGAG+AGG + chr7_3:6529936-6529955 None:intergenic 30.0%
!!! AGGTAGTTGTGATTAGTTTT+TGG - chr7_3:6529977-6529996 Msa1091220:intron 30.0%
ATAGAATGCTATAAGCCATA+AGG - chr7_3:6530533-6530552 Msa1091220:intron 30.0%
TTACTAAATAGACATCTAGC+TGG - chr7_3:6530569-6530588 Msa1091220:intron 30.0%
AACGTGAAGTGATTTATGTT+TGG - chr7_3:6530674-6530693 Msa1091220:intron 30.0%
AGTCGAAAGCTACAAATATT+AGG - chr7_3:6530751-6530770 Msa1091220:intron 30.0%
CGATGAGAAAAAGGAATAAT+AGG - chr7_3:6530953-6530972 Msa1091220:intron 30.0%
AGAAAAAGGAATAATAGGTG+TGG - chr7_3:6530958-6530977 Msa1091220:intron 30.0%
GATGAATTCTAATTGGATAC+TGG - chr7_3:6531121-6531140 Msa1091220:intron 30.0%
AACACTAGGTATCATCAAAA+GGG + chr7_3:6531186-6531205 None:intergenic 30.0%
AAACACTAGGTATCATCAAA+AGG + chr7_3:6531187-6531206 None:intergenic 30.0%
!! AATTGATGGTGTTTGGTAAA+TGG - chr7_3:6531276-6531295 Msa1091220:intron 30.0%
! TTTTATAGTGATGTCCAATG+AGG - chr7_3:6531460-6531479 Msa1091220:intron 30.0%
CAAAAATACTAGAACCTCAT+TGG + chr7_3:6531477-6531496 None:intergenic 30.0%
CGATAATGATGACTTCTAAT+TGG - chr7_3:6531535-6531554 Msa1091220:intron 30.0%
! AGTTTTACACTGATGTTCAA+CGG - chr7_3:6531736-6531755 Msa1091220:intron 30.0%
GTGTACTATTATGTAATGCT+TGG - chr7_3:6532215-6532234 Msa1091220:intron 30.0%
GTACTATTATGTAATGCTTG+GGG - chr7_3:6532217-6532236 Msa1091220:intron 30.0%
ATGAGTCATACTCAACTAAT+TGG - chr7_3:6532365-6532384 Msa1091220:intron 30.0%
TGAGTCATACTCAACTAATT+GGG - chr7_3:6532366-6532385 Msa1091220:intron 30.0%
AAGCTCTCTCATATAGATTA+CGG + chr7_3:6532423-6532442 None:intergenic 30.0%
AGAAACCACAAAATTTCTGA+TGG + chr7_3:6532552-6532571 None:intergenic 30.0%
GTGAAGCTTATATTAAAAGC+AGG - chr7_3:6532587-6532606 Msa1091220:intron 30.0%
TTGATCATGATAGACAAGAT+TGG + chr7_3:6532792-6532811 None:intergenic 30.0%
ATCAATCATGCAAACAGAAT+TGG - chr7_3:6532807-6532826 Msa1091220:intron 30.0%
!!! TTTTTTGTTATGCTAGGCTT+TGG - chr7_3:6532900-6532919 Msa1091220:intron 30.0%
CGGAAGTTATATTCTTTCTT+TGG - chr7_3:6533002-6533021 Msa1091220:intron 30.0%
!! TTTCTTTGGTCTTTCTTTTC+TGG - chr7_3:6533016-6533035 Msa1091220:intron 30.0%
TGAGTGTAATATCAGCAATA+AGG + chr7_3:6533340-6533359 None:intergenic 30.0%
CGGAATAGCTAAATATGTTA+AGG + chr7_3:6533569-6533588 None:intergenic 30.0%
GCTATTCCGAATTATATCAT+TGG - chr7_3:6533580-6533599 Msa1091220:intron 30.0%
CCTTTCCCAATGATATAATT+CGG + chr7_3:6533589-6533608 None:intergenic 30.0%
CGAATTATATCATTGGGAAA+GGG - chr7_3:6533587-6533606 Msa1091220:intron 30.0%
TATTCCAGTGAAGCTATAAA+GGG + chr7_3:6533666-6533685 None:intergenic 30.0%
! TTGCTTCTTTGACTTTACTT+TGG - chr7_3:6533720-6533739 Msa1091220:intron 30.0%
GTTTATAGGATGAATGAATG+AGG - chr7_3:6533784-6533803 Msa1091220:intron 30.0%
!! ATGCCTTTAAAGTGAAATGT+TGG + chr7_3:6533878-6533897 None:intergenic 30.0%
! ATTTCACTTTAAAGGCATGT+CGG - chr7_3:6533880-6533899 Msa1091220:intron 30.0%
GTTAATAATGTATTGTCCTC+TGG - chr7_3:6534084-6534103 Msa1091220:intron 30.0%
CATAAATTCAGATCAGATCA+GGG + chr7_3:6534144-6534163 None:intergenic 30.0%
ACATAAATTCAGATCAGATC+AGG + chr7_3:6534145-6534164 None:intergenic 30.0%
!!! TATGTCATTTTGAGTTCTTC+AGG - chr7_3:6534160-6534179 Msa1091220:intron 30.0%
! TACTGAAGTTAGGTTCTTTT+TGG - chr7_3:6534341-6534360 Msa1091220:intron 30.0%
ATGTATTCTGTAAGATCATG+GGG + chr7_3:6534398-6534417 None:intergenic 30.0%
GATGTATTCTGTAAGATCAT+GGG + chr7_3:6534399-6534418 None:intergenic 30.0%
AGATGTATTCTGTAAGATCA+TGG + chr7_3:6534400-6534419 None:intergenic 30.0%
!!! GCTCTAAGAAATCTCAATTT+TGG - chr7_3:6534460-6534479 Msa1091220:CDS 30.0%
! AATTTCCGGTACTGAAATTT+GGG + chr7_3:6534556-6534575 None:intergenic 30.0%
! AAATTTCCGGTACTGAAATT+TGG + chr7_3:6534557-6534576 None:intergenic 30.0%
TCTTTGTCAGAAGAAATTTC+CGG + chr7_3:6534570-6534589 None:intergenic 30.0%
GTTTCAAGGAAAATAGCAGA+AGG + chr7_3:6529436-6529455 None:intergenic 35.0%
!!! TCTGCTATTTTCCTTGAAAC+TGG - chr7_3:6529436-6529455 Msa1091220:CDS 35.0%
AATCATATGTTACCTGCCAA+TGG + chr7_3:6529542-6529561 None:intergenic 35.0%
TCAACAACACTTCTAATGGA+AGG + chr7_3:6529702-6529721 None:intergenic 35.0%
AGCATCAACAACACTTCTAA+TGG + chr7_3:6529706-6529725 None:intergenic 35.0%
AGAATTAAAAAAGGGCAACC+CGG - chr7_3:6529749-6529768 Msa1091220:CDS 35.0%
TCTTGTGTAAAAACAGGGTA+AGG + chr7_3:6529841-6529860 None:intergenic 35.0%
!!! TTACCCTGTTTTTACACAAG+AGG - chr7_3:6529840-6529859 Msa1091220:intron 35.0%
!! AACTTATAGCGGATAGTGTT+TGG - chr7_3:6530055-6530074 Msa1091220:intron 35.0%
!! AACTTATAGCGGATAGTGTT+TGG - chr7_3:6530055-6530074 Msa1091220:intron 35.0%
AGCTTATGATGTGAATGATG+AGG - chr7_3:6531041-6531060 Msa1091220:intron 35.0%
TGTGAATGATGAGGTCTAAT+TGG - chr7_3:6531050-6531069 Msa1091220:intron 35.0%
AGGAAGTAAGGATCAATTGA+TGG - chr7_3:6531262-6531281 Msa1091220:intron 35.0%
!! AAGGATCAATTGATGGTGTT+TGG - chr7_3:6531269-6531288 Msa1091220:intron 35.0%
GATCTGTTGACAGTGTAAAA+CGG - chr7_3:6531396-6531415 Msa1091220:intron 35.0%
! GTTTTACACTGATGTTCAAC+GGG - chr7_3:6531737-6531756 Msa1091220:intron 35.0%
ATAGGAAGTTAGGATTTAGC+AGG - chr7_3:6531931-6531950 Msa1091220:intron 35.0%
AGTTAGGATTTAGCAGGTTT+AGG - chr7_3:6531937-6531956 Msa1091220:intron 35.0%
GTTAGGATTTAGCAGGTTTA+GGG - chr7_3:6531938-6531957 Msa1091220:intron 35.0%
TTAGGATTTAGCAGGTTTAG+GGG - chr7_3:6531939-6531958 Msa1091220:intron 35.0%
CAAGTTGCGTTTAAATACTG+CGG - chr7_3:6532250-6532269 Msa1091220:intron 35.0%
GAGTCATACTCAACTAATTG+GGG - chr7_3:6532367-6532386 Msa1091220:intron 35.0%
AGTCATACTCAACTAATTGG+GGG - chr7_3:6532368-6532387 Msa1091220:intron 35.0%
! ATAGGCCATCAGAAATTTTG+TGG - chr7_3:6532544-6532563 Msa1091220:intron 35.0%
CCTTTCACAGAGACAATATT+AGG + chr7_3:6532844-6532863 None:intergenic 35.0%
CCTAATATTGTCTCTGTGAA+AGG - chr7_3:6532841-6532860 Msa1091220:intron 35.0%
TTGGTTTCTCATCCATAGTT+GGG - chr7_3:6533503-6533522 Msa1091220:intron 35.0%
CATGTAAGAAATCCCAACTA+TGG + chr7_3:6533518-6533537 None:intergenic 35.0%
CCGAATTATATCATTGGGAA+AGG - chr7_3:6533586-6533605 Msa1091220:intron 35.0%
CTATTCCAGTGAAGCTATAA+AGG + chr7_3:6533667-6533686 None:intergenic 35.0%
GCAATAACTTAAACTGGCAA+TGG + chr7_3:6533704-6533723 None:intergenic 35.0%
!! CCTTTAAAGTGAAATGTTGG+TGG + chr7_3:6533875-6533894 None:intergenic 35.0%
CCACCAACATTTCACTTTAA+AGG - chr7_3:6533872-6533891 Msa1091220:intron 35.0%
ATGCCTTAAATGTGAAGTGT+CGG + chr7_3:6533946-6533965 None:intergenic 35.0%
! ACTTCACATTTAAGGCATGT+CGG - chr7_3:6533948-6533967 Msa1091220:intron 35.0%
!! ATAAAGCACTGTCAAAGATC+TGG + chr7_3:6534036-6534055 None:intergenic 35.0%
AGATCAGGGAAGATAAAAAG+AGG + chr7_3:6534130-6534149 None:intergenic 35.0%
! AGTTCTTCAGGTTAGTATTC+AGG - chr7_3:6534172-6534191 Msa1091220:intron 35.0%
TTAATGATATAGGATGGCAC+AGG + chr7_3:6534306-6534325 None:intergenic 35.0%
TTTCTTCTTCCTACCATTGT+CGG - chr7_3:6534366-6534385 Msa1091220:intron 35.0%
!!! TCTTACTTTTTCCCCATAGT+TGG - chr7_3:6534483-6534502 Msa1091220:CDS 35.0%
ATATGATCGGAGTAGTAAGG+CGG + chr7_3:6529278-6529297 None:intergenic 40.0%
TTACTACTCCGATCATATGG+CGG - chr7_3:6529280-6529299 Msa1091220:three_prime_UTR 40.0%
GTGTGAATAGCGATGTAAAG+TGG + chr7_3:6529391-6529410 None:intergenic 40.0%
!! GAATAGAAGCACCAGTTTCA+AGG + chr7_3:6529450-6529469 None:intergenic 40.0%
ACTGGTGCTTCTATTCTCTT+TGG - chr7_3:6529454-6529473 Msa1091220:CDS 40.0%
GTGCTTCTATTCTCTTTGGT+TGG - chr7_3:6529458-6529477 Msa1091220:CDS 40.0%
GCTTCCTTATCTTTCTCCAT+TGG - chr7_3:6529523-6529542 Msa1091220:CDS 40.0%
CGTACAATACACCAAATGGT+GGG + chr7_3:6529815-6529834 None:intergenic 40.0%
GATGTTGTCATGTGACTGTA+AGG + chr7_3:6529891-6529910 None:intergenic 40.0%
GGAGGCAGAATCAATTCTAT+CGG - chr7_3:6530793-6530812 Msa1091220:intron 40.0%
TAGTGTTAGAACAGCAAAGC+TGG - chr7_3:6532094-6532113 Msa1091220:intron 40.0%
TTACGTCTCCGTCAATAGAT+TGG - chr7_3:6532185-6532204 Msa1091220:intron 40.0%
CTCAGTAGCCAATCTATTGA+CGG + chr7_3:6532196-6532215 None:intergenic 40.0%
TAATAGGTCAAACCCTAGAG+CGG + chr7_3:6532330-6532349 None:intergenic 40.0%
ATACTCAACTAATTGGGGGA+TGG - chr7_3:6532372-6532391 Msa1091220:intron 40.0%
ACTATGGATGAGAAACCAAG+AGG + chr7_3:6533502-6533521 None:intergenic 40.0%
CTTGGTTTCTCATCCATAGT+TGG - chr7_3:6533502-6533521 Msa1091220:intron 40.0%
CATGGACTCAACATGCTTAA+AGG + chr7_3:6533643-6533662 None:intergenic 40.0%
!! AAAGTGAAATGTTGGTGGCT+CGG + chr7_3:6533870-6533889 None:intergenic 40.0%
! GGAAGTTTGTTCTTCTTGCT+CGG - chr7_3:6534274-6534293 Msa1091220:intron 40.0%
ACATCTATAGGAAGTCTTGC+TGG - chr7_3:6534414-6534433 Msa1091220:intron 40.0%
TTGAGATTTCTTAGAGCCAG+AGG + chr7_3:6534457-6534476 None:intergenic 40.0%
TTCGTCCCAAATTTCAGTAC+CGG - chr7_3:6534548-6534567 Msa1091220:CDS 40.0%
! TCAATAACATAATGGCGGGG+TGG - chr7_3:6529221-6529240 Msa1091220:three_prime_UTR 45.0%
GCCTTACTACTCCGATCATA+TGG - chr7_3:6529277-6529296 Msa1091220:three_prime_UTR 45.0%
GCCATATGATCGGAGTAGTA+AGG + chr7_3:6529281-6529300 None:intergenic 45.0%
TTGCTTCTCCGCCATATGAT+CGG + chr7_3:6529291-6529310 None:intergenic 45.0%
! TCATATGGCGGAGAAGCAAT+CGG - chr7_3:6529292-6529311 Msa1091220:three_prime_UTR 45.0%
AGAAAGATAAGGAAGCAGCG+AGG + chr7_3:6529519-6529538 None:intergenic 45.0%
CCTGCCAATGGAGAAAGATA+AGG + chr7_3:6529530-6529549 None:intergenic 45.0%
CCTTATCTTTCTCCATTGGC+AGG - chr7_3:6529527-6529546 Msa1091220:CDS 45.0%
GCGTACAATACACCAAATGG+TGG + chr7_3:6529816-6529835 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG + chr7_3:6529819-6529838 None:intergenic 45.0%
CGGCCTCTTGTGTAAAAACA+GGG + chr7_3:6529846-6529865 None:intergenic 45.0%
ACGGCCTCTTGTGTAAAAAC+AGG + chr7_3:6529847-6529866 None:intergenic 45.0%
ACGGGTTTAAGTCCTGGAAA+CGG + chr7_3:6529866-6529885 None:intergenic 45.0%
TCATGTGACTGTAAGGTCAC+GGG + chr7_3:6529884-6529903 None:intergenic 45.0%
GTCATGTGACTGTAAGGTCA+CGG + chr7_3:6529885-6529904 None:intergenic 45.0%
CATCTTTACCAGTTGCGCTA+AGG - chr7_3:6529907-6529926 Msa1091220:intron 45.0%
CATCTTTACCAGTTGCGCTA+AGG - chr7_3:6529907-6529926 Msa1091220:intron 45.0%
!! TTAGCAGGTTTAGGGGTGTT+TGG - chr7_3:6531946-6531965 Msa1091220:intron 45.0%
GACGGAGACGTAAGCATAAT+TGG + chr7_3:6532178-6532197 None:intergenic 45.0%
CTAAAAGTAGGCAGACGGTT+CGG + chr7_3:6532296-6532315 None:intergenic 45.0%
TGGAGCTAAAAGTAGGCAGA+CGG + chr7_3:6532301-6532320 None:intergenic 45.0%
GAGCAAGTGGAGCTAAAAGT+AGG + chr7_3:6532308-6532327 None:intergenic 45.0%
AAAGGACTGCTGGGAACATT+AGG - chr7_3:6532859-6532878 Msa1091220:intron 45.0%
! TCTATGGTAATGAAGGGAGC+TGG - chr7_3:6532944-6532963 Msa1091220:intron 45.0%
CAGTGAAGCTATAAAGGGCA+TGG + chr7_3:6533661-6533680 None:intergenic 45.0%
CATGCCCTTTATAGCTTCAC+TGG - chr7_3:6533659-6533678 Msa1091220:intron 45.0%
!! GTCGGTGCTACAGGATAAAT+TGG + chr7_3:6533928-6533947 None:intergenic 45.0%
! GCACCGACACTTCACATTTA+AGG - chr7_3:6533940-6533959 Msa1091220:intron 45.0%
CAGGAATTCAACCTCAGCAA+TGG - chr7_3:6534191-6534210 Msa1091220:intron 45.0%
ATTCAACCTCAGCAATGGAC+TGG - chr7_3:6534196-6534215 Msa1091220:intron 45.0%
! TTCAACCTCAGCAATGGACT+GGG - chr7_3:6534197-6534216 Msa1091220:intron 45.0%
CCTAACTTCAGTAGCAGCTA+AGG + chr7_3:6534334-6534353 None:intergenic 45.0%
CCTTAGCTGCTACTGAAGTT+AGG - chr7_3:6534331-6534350 Msa1091220:intron 45.0%
TCTGTTAGTAAACTGGTGCG+TGG - chr7_3:6534653-6534672 Msa1091220:CDS 45.0%
TCGATCGGTAAACTGAAGGA+CGG + chr7_3:6534693-6534712 None:intergenic 45.0%
CAGTTCGATCGGTAAACTGA+AGG + chr7_3:6534697-6534716 None:intergenic 45.0%
TACCGATCGAACTGTCTGTT+TGG - chr7_3:6534703-6534722 Msa1091220:CDS 45.0%
ACCGATCGAACTGTCTGTTT+GGG - chr7_3:6534704-6534723 Msa1091220:CDS 45.0%
TCCCAAACAGACAGTTCGAT+CGG + chr7_3:6534708-6534727 None:intergenic 45.0%
! ATAACATAATGGCGGGGTGG+TGG - chr7_3:6529224-6529243 Msa1091220:three_prime_UTR 50.0%
TTACACAAGAGGCCGTTTCC+AGG - chr7_3:6529851-6529870 Msa1091220:intron 50.0%
AAGGTCACGGGTTTAAGTCC+TGG + chr7_3:6529872-6529891 None:intergenic 50.0%
AGAGGTAACCTTAGCGCAAC+TGG + chr7_3:6529918-6529937 None:intergenic 50.0%
AGAGGTAACCTTAGCGCAAC+TGG + chr7_3:6529918-6529937 None:intergenic 50.0%
! ACTGGTTTCTCCTGTACCAG+AGG - chr7_3:6532727-6532746 Msa1091220:intron 50.0%
CTTATTCCCTCTGCTTCCTC+TGG + chr7_3:6532746-6532765 None:intergenic 50.0%
TGTCTCTGTGAAAGGACTGC+TGG - chr7_3:6532849-6532868 Msa1091220:intron 50.0%
GTCTCTGTGAAAGGACTGCT+GGG - chr7_3:6532850-6532869 Msa1091220:intron 50.0%
!! ATGTGAAGTGTCGGTGCTAC+AGG + chr7_3:6533937-6533956 None:intergenic 50.0%
CTCGCTCAACTCCATTTGCA+AGG + chr7_3:6534248-6534267 None:intergenic 50.0%
GCAAATGGAGTTGAGCGAGA+TGG - chr7_3:6534249-6534268 Msa1091220:intron 50.0%
ATGGAGTTGAGCGAGATGGT+TGG - chr7_3:6534253-6534272 Msa1091220:intron 50.0%
GGCGGAGAAGCAATCGGAAT+CGG - chr7_3:6529298-6529317 Msa1091220:three_prime_UTR 55.0%
CGACGTGTTCGTGTCTGTGT+CGG + chr7_3:6529620-6529639 None:intergenic 55.0%
ACTGAAGCTCCCGCATACAC+AGG - chr7_3:6529775-6529794 Msa1091220:intron 55.0%
CTGAAGCTCCCGCATACACA+GGG - chr7_3:6529776-6529795 Msa1091220:intron 55.0%
TTACCGGACCCTGTGTATGC+GGG + chr7_3:6529787-6529806 None:intergenic 55.0%
CTTACCGGACCCTGTGTATG+CGG + chr7_3:6529788-6529807 None:intergenic 55.0%
GCATACACAGGGTCCGGTAA+GGG - chr7_3:6529787-6529806 Msa1091220:intron 55.0%
CATACACAGGGTCCGGTAAG+GGG - chr7_3:6529788-6529807 Msa1091220:intron 55.0%
! CAAATGGTGGGACCCCTTAC+CGG + chr7_3:6529803-6529822 None:intergenic 55.0%
! AAAGTAGGCAGACGGTTCGG+CGG + chr7_3:6532293-6532312 None:intergenic 55.0%
AAACCCTAGAGCGGAGCAAG+TGG + chr7_3:6532321-6532340 None:intergenic 55.0%
TCCTGTACCAGAGGAAGCAG+AGG - chr7_3:6532736-6532755 Msa1091220:intron 55.0%
CCTGTACCAGAGGAAGCAGA+GGG - chr7_3:6532737-6532756 Msa1091220:intron 55.0%
TCTAGCCCAGTCCATTGCTG+AGG + chr7_3:6534205-6534224 None:intergenic 55.0%
!! CAGCAATGGACTGGGCTAGA+AGG - chr7_3:6534205-6534224 Msa1091220:intron 55.0%
GGGATGCATCCGACAATGGT+AGG + chr7_3:6534378-6534397 None:intergenic 55.0%
CATGGGGATGCATCCGACAA+TGG + chr7_3:6534382-6534401 None:intergenic 55.0%
! ACATAATGGCGGGGTGGTGG+TGG - chr7_3:6529227-6529246 Msa1091220:three_prime_UTR 60.0%
!! GGGGTGGTGGTGGAAAACAG+CGG - chr7_3:6529237-6529256 Msa1091220:three_prime_UTR 60.0%
ATGCGGGAGCTTCAGTGCAC+CGG + chr7_3:6529771-6529790 None:intergenic 60.0%
CGCATACACAGGGTCCGGTA+AGG - chr7_3:6529786-6529805 Msa1091220:intron 60.0%
CGGTAAGGGGTCCCACCATT+TGG - chr7_3:6529801-6529820 Msa1091220:intron 60.0%
AGCTCCACTTGCTCCGCTCT+AGG - chr7_3:6532314-6532333 Msa1091220:intron 60.0%
GCTCCACTTGCTCCGCTCTA+GGG - chr7_3:6532315-6532334 Msa1091220:intron 60.0%
CCCTCTGCTTCCTCTGGTAC+AGG + chr7_3:6532740-6532759 None:intergenic 60.0%
GCAGCTGTTGCTACAGCCTC+TGG - chr7_3:6534438-6534457 Msa1091220:CDS 60.0%
TGCGGGAGCTTCAGTGCACC+GGG + chr7_3:6529770-6529789 None:intergenic 65.0%
GCTCCCGCATACACAGGGTC+CGG - chr7_3:6529781-6529800 Msa1091220:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 6529116 6534855 6529116 ID=Msa1091220;Name=Msa1091220
chr7_3 mRNA 6529116 6534855 6529116 ID=Msa1091220-mRNA-1;Parent=Msa1091220;Name=Msa1091220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=116|1|1|1|1|1|2|235|251
chr7_3 exon 6529116 6529789 6529116 ID=Msa1091220-mRNA-1:exon:1952;Parent=Msa1091220-mRNA-1
chr7_3 exon 6534423 6534855 6534423 ID=Msa1091220-mRNA-1:exon:1951;Parent=Msa1091220-mRNA-1
chr7_3 five_prime_UTR 6534740 6534855 6534740 ID=Msa1091220-mRNA-1:five_prime_utr;Parent=Msa1091220-mRNA-1
chr7_3 CDS 6534423 6534739 6534423 ID=Msa1091220-mRNA-1:cds;Parent=Msa1091220-mRNA-1
chr7_3 CDS 6529351 6529789 6529351 ID=Msa1091220-mRNA-1:cds;Parent=Msa1091220-mRNA-1
chr7_3 three_prime_UTR 6529116 6529350 6529116 ID=Msa1091220-mRNA-1:three_prime_utr;Parent=Msa1091220-mRNA-1
Gene Sequence

>Msa1091220

ATGGCGGGGTGGTGGTGGAAAACAGCGGTGTTAATAGCAATATCCGCCTTACTACTCCGATCATATGGCGGAGAAGCAATCGGAATCGGAATCGATAAAGAATCATTGATGAAATGGATGAGAGATTTATCTGATAAACTAGGAATTTGGGCGATTCCACTTTACATCGCTATTCACACAATTTCAATTGCTCTTTGTTTACCTTCTGCTATTTTCCTTGAAACTGGTGCTTCTATTCTCTTTGGTTGGTTTGCTTCTGTTCTTTGTGTTTTCTCTGCTAAGATCCTCGCTGCTTCCTTATCTTTCTCCATTGGCAGGTTAGTATTCAGGAATTCAACCTCAGCAATGGACTGGGCTAGAAGGAACAAATATTTTAAAATCCTTGCAAATGGAGTTGAGCGAGATGGTTGGAAGTTTGTTCTTCTTGCTCGGTTCTCGCCTGTGCCATCCTATATCATTAACTATACCTTAGCTGCTACTGAAGTTAGGTTCTTTTTGGATTTTCTTCTTCCTACCATTGTCGGATGCATCCCCATGATCTTACAGAATACATCTATAGGAAGTCTTGCTGGTGCAGCTGTTGCTACAGCCTCTGGCTCTAAGAAATCTCAATTTTGGTCTTACTTTTTCCCCATAGTTGGTATTTTATCTAGTGTACTTATTTCTTTGAGAATTAAAAAGTATTCGTCCCAAATTTCAGTACCGGAAATTTCTTCTGACAAAGATAACACTGTTGAGTCAAAACAACAAAAATAA
Protein sequence

>Msa1091220

MAGWWWKTAVLIAISALLLRSYGGEAIGIGIDKESLMKWMRDLSDKLGIWAIPLYIAIHTISIALCLPSAIFLETGASILFGWFASVLCVFSAKILAASLSFSIGRLVFRNSTSAMDWARRNKYFKILANGVERDGWKFVLLARFSPVPSYIINYTLAATEVRFFLDFLLPTIVGCIPMILQNTSIGSLAGAAVATASGSKKSQFWSYFFPIVGILSSVLISLRIKKYSSQISVPEISSDKDNTVESKQQK*