AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1136700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1136700 MtrunA17_Chr3g0130651 91.667 96 7 1 38 133 44 138 1.00e-54 177
Msa1136700 MtrunA17_Chr3g0130671 68.235 85 27 0 49 133 67 151 1.99e-35 123
Msa1136700 MtrunA17_Chr3g0125091 66.667 96 31 1 38 133 52 146 4.55e-34 123
Msa1136700 MtrunA17_Chr3g0125081 66.667 96 31 1 38 133 52 146 1.04e-33 122
Msa1136700 MtrunA17_Chr8g0384661 64.211 95 33 1 38 132 68 161 2.05e-33 121
Msa1136700 MtrunA17_Chr3g0130641 70.238 84 25 0 50 133 62 145 6.27e-33 120
Msa1136700 MtrunA17_Chr3g0125101 64.583 96 33 1 38 133 52 146 1.59e-32 119
Msa1136700 MtrunA17_Chr1g0159761 59.375 96 38 1 38 133 45 139 4.52e-31 115
Msa1136700 MtrunA17_Chr5g0402151 63.095 84 26 1 49 132 28 106 1.83e-28 105
Msa1136700 MtrunA17_Chr4g0037201 55.208 96 42 1 38 133 77 171 3.70e-28 107
Msa1136700 MtrunA17_Chr1g0146491 55.208 96 42 1 38 133 41 135 8.53e-27 103
Msa1136700 MtrunA17_Chr3g0140761 53.125 96 44 1 38 133 51 145 1.65e-26 102
Msa1136700 MtrunA17_Chr1g0206821 54.167 96 43 1 38 133 40 134 2.26e-24 97.1
Msa1136700 MtrunA17_Chr1g0146351 56.627 83 35 1 48 130 30 111 2.63e-24 90.1
Msa1136700 MtrunA17_Chr1g0206871 53.125 96 44 1 38 133 33 127 1.01e-23 94.7
Msa1136700 MtrunA17_Chr1g0146411 56.627 83 35 1 48 130 60 141 1.26e-21 89.0
Msa1136700 MtrunA17_Chr3g0140531 51.765 85 41 0 49 133 52 136 2.20e-21 88.2
Msa1136700 MtrunA17_Chr1g0212251 85.106 47 7 0 1 47 65 111 4.80e-20 84.7
Msa1136700 MtrunA17_Chr3g0140541 49.412 85 43 0 49 133 53 137 1.01e-19 83.6
Msa1136700 MtrunA17_Chr1g0146391 48.810 84 43 0 49 132 40 123 1.90e-19 82.8
Msa1136700 MtrunA17_Chr2g0293331 47.619 84 44 0 49 132 40 123 3.39e-19 82.0
Msa1136700 MtrunA17_Chr1g0212201 85.106 47 7 0 1 47 69 115 4.33e-19 82.0
Msa1136700 MtrunA17_Chr1g0146331 45.238 84 46 0 49 132 48 131 1.11e-18 80.9
Msa1136700 MtrunA17_Chr1g0212291 100.000 47 0 0 1 47 66 112 2.39e-16 74.3
Msa1136700 MtrunA17_Chr1g0206841 56.364 55 24 0 49 103 49 103 3.92e-15 66.2
Msa1136700 MtrunA17_Chr3g0103261 38.554 83 51 0 50 132 52 134 6.42e-15 67.4
Msa1136700 MtrunA17_Chr3g0103221 41.667 84 49 0 49 132 58 141 7.59e-15 69.7
Msa1136700 MtrunA17_Chr3g0103231 39.759 83 50 0 50 132 52 134 1.41e-14 68.9
Msa1136700 MtrunA17_Chr8g0390931 63.265 49 18 0 2 50 89 137 3.94e-14 67.8
Msa1136700 MtrunA17_Chr3g0103211 39.175 97 55 1 40 132 38 134 6.56e-14 67.0
Msa1136700 MtrunA17_Chr4g0054741 78.378 37 8 0 1 37 77 113 1.23e-13 66.2
Msa1136700 MtrunA17_Chr4g0054761 77.778 36 8 0 2 37 78 113 4.25e-13 62.4
Msa1136700 MtrunA17_Chr5g0447221 35.714 98 57 3 40 132 44 140 1.95e-12 62.8
Msa1136700 MtrunA17_Chr7g0259731 44.444 81 34 2 1 70 84 164 2.10e-12 62.8
Msa1136700 MtrunA17_Chr6g0484691 63.043 46 17 0 5 50 116 161 4.17e-12 62.0
Msa1136700 MtrunA17_Chr1g0206861 41.111 90 51 2 38 126 39 127 5.17e-12 59.3
Msa1136700 MtrunA17_Chr3g0103271 37.647 85 52 1 49 132 55 139 5.71e-12 61.6
Msa1136700 MtrunA17_Chr4g0025591 50.000 68 29 1 2 64 70 137 9.65e-12 60.8
Msa1136700 MtrunA17_Chr1g0188871 52.727 55 25 1 1 55 74 127 1.66e-11 60.1
Msa1136700 MtrunA17_Chr2g0321971 63.830 47 17 0 1 47 65 111 3.21e-11 59.3
Msa1136700 MtrunA17_Chr1g0188881 54.545 55 24 1 1 55 80 133 3.25e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1136700 AT5G09220.1 60.417 96 37 1 38 133 63 157 6.95e-33 120
Msa1136700 AT5G63850.1 57.292 96 40 1 38 133 36 130 1.23e-32 119
Msa1136700 AT1G77380.1 67.857 84 27 0 50 133 58 141 3.17e-32 118
Msa1136700 AT1G44100.1 57.292 96 40 1 38 133 45 139 1.46e-29 111
Msa1136700 AT5G49630.1 56.250 96 41 1 38 133 50 144 3.40e-27 105
Msa1136700 AT1G58360.1 51.042 96 46 1 38 133 54 148 1.26e-25 100
Msa1136700 AT1G10010.1 51.042 96 46 1 38 133 45 139 1.10e-22 92.4
Msa1136700 AT1G10010.2 51.042 96 46 1 38 133 46 140 1.15e-22 92.4
Msa1136700 AT4G17970.1 78.723 47 10 0 1 47 67 113 2.77e-18 80.1
Msa1136700 AT5G46610.1 54.321 81 24 2 1 69 69 148 7.48e-18 79.0
Msa1136700 AT5G46600.1 53.086 81 25 2 1 69 70 149 1.03e-16 75.5
Msa1136700 AT5G23810.5 39.130 92 51 2 40 126 40 131 2.95e-14 67.0
Msa1136700 AT5G23810.3 39.130 92 51 2 40 126 40 131 2.95e-14 67.0
Msa1136700 AT5G23810.4 39.130 92 51 2 40 126 40 131 4.19e-14 67.4
Msa1136700 AT5G23810.2 39.130 92 51 2 40 126 40 131 8.16e-14 67.0
Msa1136700 AT5G23810.1 39.130 92 51 2 40 126 40 131 8.25e-14 67.4
Msa1136700 AT1G08440.1 57.377 61 24 1 2 60 42 102 2.41e-12 63.2
Msa1136700 AT4G00910.1 49.315 73 27 1 1 63 86 158 4.00e-12 62.4

Find 77 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGTCTGTGTGTTGGTTTC+TGG 0.016885 7_4:-5439619 Msa1136700:five_prime_UTR
GCTCAGTTGGTTTGGGTAAT+TGG 0.223474 7_4:-5439022 Msa1136700:CDS
CTACATGTTGAAGGCTAGTT+TGG 0.257150 7_4:+5439775 None:intergenic
TGTTTCATTGTTGTATCTAA+TGG 0.262149 7_4:-5439321 Msa1136700:exon
TTTGCGGTTTCGTCGGATCT+TGG 0.277905 7_4:-5439647 Msa1136700:five_prime_UTR
TTGTAGAGACAAGTCCAATT+TGG 0.299577 7_4:+5439904 None:intergenic
AACTCTGTCAGCAGGATTAT+TGG 0.318252 7_4:-5439050 Msa1136700:CDS
GAAGCTGTTCACACAATTCT+AGG 0.331709 7_4:-5438887 Msa1136700:intron
GTTCAGTACACAAATCTTTA+CGG 0.334346 7_4:-5438258 Msa1136700:CDS
TGGTCATAGCTCAGTTGGTT+TGG 0.340762 7_4:-5439030 Msa1136700:CDS
TTAGCTGAATGTTATCGAAT+TGG 0.348772 7_4:-5438947 Msa1136700:CDS
TTGCGGTTTCGTCGGATCTT+GGG 0.360644 7_4:-5439646 Msa1136700:five_prime_UTR
AAAGGACTTAATAGAGGATT+AGG 0.369561 7_4:-5439073 Msa1136700:CDS
TTACCTCTTTCGATTCTGTT+TGG 0.376672 7_4:+5439449 None:intergenic
GGTCATAGCTCAGTTGGTTT+GGG 0.381927 7_4:-5439029 Msa1136700:CDS
CGAATTGGTGATCCTCATTA+CGG 0.382440 7_4:-5438932 Msa1136700:CDS
TCTATTAAGTCCTTTGCATA+AGG 0.384312 7_4:+5439081 None:intergenic
TCTCTTGCTCCTGCTTGATT+CGG 0.386260 7_4:+5439594 None:intergenic
CCTTCAACATGTAGATCCTT+TGG 0.390436 7_4:-5439766 Msa1136700:five_prime_UTR
AATCTTTACGGAACTGCAAT+AGG 0.394383 7_4:-5438246 Msa1136700:CDS
GTTTCGTCGGATCTTGGGTT+TGG 0.402535 7_4:-5439641 Msa1136700:five_prime_UTR
CCAATCATTCATTCACGAAA+AGG 0.404191 7_4:-5439559 Msa1136700:five_prime_UTR
CGTTCTTCTTCCTCACGAAT+CGG 0.410511 7_4:+5439501 None:intergenic
TGAAGGCTAGTTTGGATTTG+AGG 0.418168 7_4:+5439783 None:intergenic
GGTTGTTTGTAGAGAGGAGG+AGG 0.421060 7_4:+5439832 None:intergenic
AAAGAGACTAGATCTAGGTT+TGG 0.424811 7_4:+5439368 None:intergenic
GATCTAGTCTCTTTGAAAGT+TGG 0.427384 7_4:-5439359 Msa1136700:five_prime_UTR
GCAATAGGATACACAATTGC+GGG 0.428317 7_4:-5438231 Msa1136700:CDS
AAAGAGGAACTATACTTTCA+TGG 0.430550 7_4:-5438909 Msa1136700:CDS
CTGACGGAGAGAAGTTGATC+TGG 0.430791 7_4:+5439679 None:intergenic
CTTTCAAAGAGACTAGATCT+AGG 0.432292 7_4:+5439363 None:intergenic
TTCTTCCTCACGAATCGGTC+GGG 0.436012 7_4:+5439506 None:intergenic
TCCTTTGGCTGGTGGTGCGA+TGG 0.445597 7_4:-5439751 Msa1136700:five_prime_UTR
TAATGGAACCATTGTACAAA+GGG 0.453257 7_4:-5439304 Msa1136700:CDS
AGCAGGAGCAAGAGAGGAAG+TGG 0.456569 7_4:-5439586 Msa1136700:five_prime_UTR
GATCACTCACATCATGCTAA+TGG 0.460901 7_4:+5438206 None:intergenic
TGGGGTTGTTTGTAGAGAGG+AGG 0.464294 7_4:+5439829 None:intergenic
GGTTTCAATGACACCCTTTG+TGG 0.466023 7_4:-5438285 Msa1136700:CDS
TAGTTCCTCTTTCCGTAATG+AGG 0.476378 7_4:+5438920 None:intergenic
CCAAAGGATCTACATGTTGA+AGG 0.485295 7_4:+5439766 None:intergenic
TTATGCAAAGGACTTAATAG+AGG 0.486073 7_4:-5439079 Msa1136700:CDS
AACAACACAAACGTTCAAAT+CGG 0.490303 7_4:-5439536 Msa1136700:five_prime_UTR
TTTCCTATCCCTTTGTACAA+TGG 0.496607 7_4:+5439296 None:intergenic
GGGTTTGGCTGTCTGTGTGT+TGG 0.497111 7_4:-5439626 Msa1136700:five_prime_UTR
AACGAGGGGCGCTGGTGGTG+CGG 0.501184 7_4:+5439720 None:intergenic
CTTCTTCCTCACGAATCGGT+CGG 0.503463 7_4:+5439505 None:intergenic
CCTTTGGCTGGTGGTGCGAT+GGG 0.513598 7_4:-5439750 Msa1136700:five_prime_UTR
CAACATGTAGATCCTTTGGC+TGG 0.521078 7_4:-5439762 Msa1136700:five_prime_UTR
GTTTGGGGTTGTTTGTAGAG+AGG 0.524595 7_4:+5439826 None:intergenic
CATGACTGTGGTTGTCGTCA+TGG 0.525879 7_4:-5439255 Msa1136700:CDS
GGATTAGGAACTCTGTCAGC+AGG 0.527709 7_4:-5439058 Msa1136700:CDS
GTTTCAATGACACCCTTTGT+GGG 0.537172 7_4:-5438284 Msa1136700:CDS
GAATCGAAAGAGGTAAATAG+AGG 0.537468 7_4:-5439442 Msa1136700:five_prime_UTR
GAACCATTGTACAAAGGGAT+AGG 0.540699 7_4:-5439299 Msa1136700:CDS
GAATCAAGCAGGAGCAAGAG+AGG 0.542256 7_4:-5439592 Msa1136700:five_prime_UTR
ACGAGAAGAACGAGGGGCGC+TGG 0.545060 7_4:+5439712 None:intergenic
ATTATTGGTCATAGCTCAGT+TGG 0.551033 7_4:-5439035 Msa1136700:CDS
GTGATCCTCATTACGGAAAG+AGG 0.563476 7_4:-5438925 Msa1136700:CDS
TTTCTGGAACCGAATCAAGC+AGG 0.568528 7_4:-5439603 Msa1136700:five_prime_UTR
AGGCTAGTTTGGATTTGAGG+AGG 0.571649 7_4:+5439786 None:intergenic
GTACTGAACTATCCCACAAA+GGG 0.571823 7_4:+5438272 None:intergenic
AAACCAAACAGAATCGAAAG+AGG 0.577658 7_4:-5439452 Msa1136700:five_prime_UTR
CTGAAGAAAATCATAACAGA+AGG 0.590235 7_4:+5438998 None:intergenic
AGAAGAACGAGGGGCGCTGG+TGG 0.591627 7_4:+5439715 None:intergenic
CATGTAGATCCTTTGGCTGG+TGG 0.594338 7_4:-5439759 Msa1136700:five_prime_UTR
CTAATGGAACCATTGTACAA+AGG 0.603339 7_4:-5439305 Msa1136700:CDS
TGCAATAGGATACACAATTG+CGG 0.626230 7_4:-5438232 Msa1136700:CDS
GAAGTACGACGAGAAGAACG+AGG 0.629101 7_4:+5439704 None:intergenic
GTCGTCATGGAGTTTACAGT+AGG 0.630287 7_4:-5439242 Msa1136700:intron
GCAGGAGGAACCTTATGCAA+AGG 0.631495 7_4:-5439091 Msa1136700:intron
AAGTACGACGAGAAGAACGA+GGG 0.638260 7_4:+5439705 None:intergenic
CCCATCGCACCACCAGCCAA+AGG 0.639211 7_4:+5439750 None:intergenic
CGATTCGTGAGGAAGAAGAA+CGG 0.665989 7_4:-5439500 Msa1136700:five_prime_UTR
TGTTGTGGCTGTCATGACTG+TGG 0.668854 7_4:-5439267 Msa1136700:CDS
TAGATCCCGACCGATTCGTG+AGG 0.670906 7_4:-5439511 Msa1136700:five_prime_UTR
TGTACTGAACTATCCCACAA+AGG 0.675008 7_4:+5438271 None:intergenic
AGTACGACGAGAAGAACGAG+GGG 0.710390 7_4:+5439706 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATAGTATAATTTTGAAA+TGG - chr7_4:5439753-5439772 Msa1136700:five_prime_UTR 10.0%
!!! TAAATAGTATAATTTTGAAA+TGG - chr7_4:5439753-5439772 Msa1136700:five_prime_UTR 10.0%
!!! TCAAGTAATATTTTAGAAAG+AGG + chr7_4:5438236-5438255 None:intergenic 20.0%
!! TAGTAGTGATTATTAAATTC+TGG + chr7_4:5439507-5439526 None:intergenic 20.0%
!! AGAATTTAATAATCACTACT+AGG - chr7_4:5439506-5439525 Msa1136700:five_prime_UTR 20.0%
!!! TCAAGTAATATTTTAGAAAG+AGG + chr7_4:5438236-5438255 None:intergenic 20.0%
!! TAGTAGTGATTATTAAATTC+TGG + chr7_4:5439507-5439526 None:intergenic 20.0%
!! AGAATTTAATAATCACTACT+AGG - chr7_4:5439506-5439525 Msa1136700:five_prime_UTR 20.0%
!!! AGAACGATTATTTTAGAAAG+AGG + chr7_4:5438261-5438280 None:intergenic 25.0%
! TGTTTCATTGTTGTATCTAA+TGG - chr7_4:5438792-5438811 Msa1136700:intron 25.0%
! TAGGTATTAAATATGATTGC+AGG - chr7_4:5439004-5439023 Msa1136700:CDS 25.0%
! ATAATCGAAACGAATCAAAT+TGG - chr7_4:5439293-5439312 Msa1136700:CDS 25.0%
!!! AGAACGATTATTTTAGAAAG+AGG + chr7_4:5438261-5438280 None:intergenic 25.0%
! TGTTTCATTGTTGTATCTAA+TGG - chr7_4:5438792-5438811 Msa1136700:intron 25.0%
! TAGGTATTAAATATGATTGC+AGG - chr7_4:5439004-5439023 Msa1136700:CDS 25.0%
! ATAATCGAAACGAATCAAAT+TGG - chr7_4:5439293-5439312 Msa1136700:CDS 25.0%
AACAACACAAACGTTCAAAT+CGG - chr7_4:5438577-5438596 Msa1136700:intron 30.0%
CGAAAAAATGTTTGAACAGA+AGG + chr7_4:5438720-5438739 None:intergenic 30.0%
TAATGGAACCATTGTACAAA+GGG - chr7_4:5438809-5438828 Msa1136700:intron 30.0%
TGTTAAGATTACAGTCTCAT+AGG - chr7_4:5438985-5439004 Msa1136700:CDS 30.0%
GTATTAAATATGATTGCAGG+AGG - chr7_4:5439007-5439026 Msa1136700:CDS 30.0%
TCTATTAAGTCCTTTGCATA+AGG + chr7_4:5439035-5439054 None:intergenic 30.0%
TTATGCAAAGGACTTAATAG+AGG - chr7_4:5439034-5439053 Msa1136700:CDS 30.0%
AAAGGACTTAATAGAGGATT+AGG - chr7_4:5439040-5439059 Msa1136700:CDS 30.0%
CTGAAGAAAATCATAACAGA+AGG + chr7_4:5439118-5439137 None:intergenic 30.0%
TTAGCTGAATGTTATCGAAT+TGG - chr7_4:5439166-5439185 Msa1136700:intron 30.0%
AAAGAGGAACTATACTTTCA+TGG - chr7_4:5439204-5439223 Msa1136700:intron 30.0%
AACTGTGAAGTAAGTGTTTA+TGG - chr7_4:5439332-5439351 Msa1136700:five_prime_UTR 30.0%
CTAGTTTAAACTGCTCAAAA+GGG + chr7_4:5439393-5439412 None:intergenic 30.0%
!!! TGACTTTGTTTTTGTGTACT+TGG + chr7_4:5439550-5439569 None:intergenic 30.0%
!! TGATTTGGTCATATACTTCT+TGG - chr7_4:5439582-5439601 Msa1136700:five_prime_UTR 30.0%
! TGAGAATCAAATGATTTTGC+AGG - chr7_4:5439804-5439823 Msa1136700:five_prime_UTR 30.0%
GTTCAGTACACAAATCTTTA+CGG - chr7_4:5439855-5439874 Msa1136700:five_prime_UTR 30.0%
AACAACACAAACGTTCAAAT+CGG - chr7_4:5438577-5438596 Msa1136700:intron 30.0%
CGAAAAAATGTTTGAACAGA+AGG + chr7_4:5438720-5438739 None:intergenic 30.0%
TAATGGAACCATTGTACAAA+GGG - chr7_4:5438809-5438828 Msa1136700:intron 30.0%
TGTTAAGATTACAGTCTCAT+AGG - chr7_4:5438985-5439004 Msa1136700:CDS 30.0%
GTATTAAATATGATTGCAGG+AGG - chr7_4:5439007-5439026 Msa1136700:CDS 30.0%
TCTATTAAGTCCTTTGCATA+AGG + chr7_4:5439035-5439054 None:intergenic 30.0%
TTATGCAAAGGACTTAATAG+AGG - chr7_4:5439034-5439053 Msa1136700:CDS 30.0%
AAAGGACTTAATAGAGGATT+AGG - chr7_4:5439040-5439059 Msa1136700:CDS 30.0%
CTGAAGAAAATCATAACAGA+AGG + chr7_4:5439118-5439137 None:intergenic 30.0%
TTAGCTGAATGTTATCGAAT+TGG - chr7_4:5439166-5439185 Msa1136700:intron 30.0%
AAAGAGGAACTATACTTTCA+TGG - chr7_4:5439204-5439223 Msa1136700:intron 30.0%
AACTGTGAAGTAAGTGTTTA+TGG - chr7_4:5439332-5439351 Msa1136700:five_prime_UTR 30.0%
CTAGTTTAAACTGCTCAAAA+GGG + chr7_4:5439393-5439412 None:intergenic 30.0%
!!! TGACTTTGTTTTTGTGTACT+TGG + chr7_4:5439550-5439569 None:intergenic 30.0%
!! TGATTTGGTCATATACTTCT+TGG - chr7_4:5439582-5439601 Msa1136700:five_prime_UTR 30.0%
! TGAGAATCAAATGATTTTGC+AGG - chr7_4:5439804-5439823 Msa1136700:five_prime_UTR 30.0%
GTTCAGTACACAAATCTTTA+CGG - chr7_4:5439855-5439874 Msa1136700:five_prime_UTR 30.0%
! AGAAAAAGTGAAGTCGGTTT+GGG + chr7_4:5438306-5438325 None:intergenic 35.0%
AAGAAAAAGTGAAGTCGGTT+TGG + chr7_4:5438307-5438326 None:intergenic 35.0%
! CCTTTTCGTGAATGAATGAT+TGG + chr7_4:5438557-5438576 None:intergenic 35.0%
CCAATCATTCATTCACGAAA+AGG - chr7_4:5438554-5438573 Msa1136700:intron 35.0%
AAACCAAACAGAATCGAAAG+AGG - chr7_4:5438661-5438680 Msa1136700:intron 35.0%
TTACCTCTTTCGATTCTGTT+TGG + chr7_4:5438667-5438686 None:intergenic 35.0%
GAATCGAAAGAGGTAAATAG+AGG - chr7_4:5438671-5438690 Msa1136700:intron 35.0%
AAAGAGACTAGATCTAGGTT+TGG + chr7_4:5438748-5438767 None:intergenic 35.0%
CTTTCAAAGAGACTAGATCT+AGG + chr7_4:5438753-5438772 None:intergenic 35.0%
! GATCTAGTCTCTTTGAAAGT+TGG - chr7_4:5438754-5438773 Msa1136700:intron 35.0%
CTAATGGAACCATTGTACAA+AGG - chr7_4:5438808-5438827 Msa1136700:intron 35.0%
TTTCCTATCCCTTTGTACAA+TGG + chr7_4:5438820-5438839 None:intergenic 35.0%
GATAGGAAAAAATGCTGTTG+TGG - chr7_4:5438831-5438850 Msa1136700:intron 35.0%
ATTATTGGTCATAGCTCAGT+TGG - chr7_4:5439078-5439097 Msa1136700:CDS 35.0%
! GCTAGTTTAAACTGCTCAAA+AGG + chr7_4:5439394-5439413 None:intergenic 35.0%
! GAATCAAATGATTTTGCAGG+AGG - chr7_4:5439807-5439826 Msa1136700:five_prime_UTR 35.0%
AATCTTTACGGAACTGCAAT+AGG - chr7_4:5439867-5439886 Msa1136700:five_prime_UTR 35.0%
TGCAATAGGATACACAATTG+CGG - chr7_4:5439881-5439900 Msa1136700:five_prime_UTR 35.0%
! AGAAAAAGTGAAGTCGGTTT+GGG + chr7_4:5438306-5438325 None:intergenic 35.0%
AAGAAAAAGTGAAGTCGGTT+TGG + chr7_4:5438307-5438326 None:intergenic 35.0%
! CCTTTTCGTGAATGAATGAT+TGG + chr7_4:5438557-5438576 None:intergenic 35.0%
CCAATCATTCATTCACGAAA+AGG - chr7_4:5438554-5438573 Msa1136700:intron 35.0%
AAACCAAACAGAATCGAAAG+AGG - chr7_4:5438661-5438680 Msa1136700:intron 35.0%
TTACCTCTTTCGATTCTGTT+TGG + chr7_4:5438667-5438686 None:intergenic 35.0%
GAATCGAAAGAGGTAAATAG+AGG - chr7_4:5438671-5438690 Msa1136700:intron 35.0%
AAAGAGACTAGATCTAGGTT+TGG + chr7_4:5438748-5438767 None:intergenic 35.0%
CTTTCAAAGAGACTAGATCT+AGG + chr7_4:5438753-5438772 None:intergenic 35.0%
! GATCTAGTCTCTTTGAAAGT+TGG - chr7_4:5438754-5438773 Msa1136700:intron 35.0%
CTAATGGAACCATTGTACAA+AGG - chr7_4:5438808-5438827 Msa1136700:intron 35.0%
TTTCCTATCCCTTTGTACAA+TGG + chr7_4:5438820-5438839 None:intergenic 35.0%
GATAGGAAAAAATGCTGTTG+TGG - chr7_4:5438831-5438850 Msa1136700:intron 35.0%
ATTATTGGTCATAGCTCAGT+TGG - chr7_4:5439078-5439097 Msa1136700:CDS 35.0%
! GCTAGTTTAAACTGCTCAAA+AGG + chr7_4:5439394-5439413 None:intergenic 35.0%
! GAATCAAATGATTTTGCAGG+AGG - chr7_4:5439807-5439826 Msa1136700:five_prime_UTR 35.0%
AATCTTTACGGAACTGCAAT+AGG - chr7_4:5439867-5439886 Msa1136700:five_prime_UTR 35.0%
TGCAATAGGATACACAATTG+CGG - chr7_4:5439881-5439900 Msa1136700:five_prime_UTR 35.0%
!! GAAAAAGTGAAGTCGGTTTG+GGG + chr7_4:5438305-5438324 None:intergenic 40.0%
GGAGGAAGAAAAAGTGAAGT+CGG + chr7_4:5438312-5438331 None:intergenic 40.0%
!! TGAAGGCTAGTTTGGATTTG+AGG + chr7_4:5438333-5438352 None:intergenic 40.0%
! CTACATGTTGAAGGCTAGTT+TGG + chr7_4:5438341-5438360 None:intergenic 40.0%
CCAAAGGATCTACATGTTGA+AGG + chr7_4:5438350-5438369 None:intergenic 40.0%
CCTTCAACATGTAGATCCTT+TGG - chr7_4:5438347-5438366 Msa1136700:intron 40.0%
CCGCAAAAACTCAAATCTGA+CGG + chr7_4:5438453-5438472 None:intergenic 40.0%
!! CCGTCAGATTTGAGTTTTTG+CGG - chr7_4:5438450-5438469 Msa1136700:intron 40.0%
! TTGAGTTTTTGCGGTTTCGT+CGG - chr7_4:5438459-5438478 Msa1136700:intron 40.0%
GAACCATTGTACAAAGGGAT+AGG - chr7_4:5438814-5438833 Msa1136700:intron 40.0%
TCATGGAGTTTACAGTAGGT+AGG - chr7_4:5438875-5438894 Msa1136700:intron 40.0%
AACTCTGTCAGCAGGATTAT+TGG - chr7_4:5439063-5439082 Msa1136700:CDS 40.0%
! TTTTCTTCAGTCTCATCACG+TGG - chr7_4:5439128-5439147 Msa1136700:intron 40.0%
! CGAATTGGTGATCCTCATTA+CGG - chr7_4:5439181-5439200 Msa1136700:intron 40.0%
TAGTTCCTCTTTCCGTAATG+AGG + chr7_4:5439196-5439215 None:intergenic 40.0%
GAAGCTGTTCACACAATTCT+AGG - chr7_4:5439226-5439245 Msa1136700:intron 40.0%
TCACACGTTTGCACTTGATT+TGG - chr7_4:5439567-5439586 Msa1136700:five_prime_UTR 40.0%
GTTTCAATGACACCCTTTGT+GGG - chr7_4:5439829-5439848 Msa1136700:five_prime_UTR 40.0%
GTACTGAACTATCCCACAAA+GGG + chr7_4:5439844-5439863 None:intergenic 40.0%
TGTACTGAACTATCCCACAA+AGG + chr7_4:5439845-5439864 None:intergenic 40.0%
GCAATAGGATACACAATTGC+GGG - chr7_4:5439882-5439901 Msa1136700:five_prime_UTR 40.0%
!! GAAAAAGTGAAGTCGGTTTG+GGG + chr7_4:5438305-5438324 None:intergenic 40.0%
GGAGGAAGAAAAAGTGAAGT+CGG + chr7_4:5438312-5438331 None:intergenic 40.0%
!! TGAAGGCTAGTTTGGATTTG+AGG + chr7_4:5438333-5438352 None:intergenic 40.0%
! CTACATGTTGAAGGCTAGTT+TGG + chr7_4:5438341-5438360 None:intergenic 40.0%
CCAAAGGATCTACATGTTGA+AGG + chr7_4:5438350-5438369 None:intergenic 40.0%
CCTTCAACATGTAGATCCTT+TGG - chr7_4:5438347-5438366 Msa1136700:intron 40.0%
CCGCAAAAACTCAAATCTGA+CGG + chr7_4:5438453-5438472 None:intergenic 40.0%
!! CCGTCAGATTTGAGTTTTTG+CGG - chr7_4:5438450-5438469 Msa1136700:intron 40.0%
! TTGAGTTTTTGCGGTTTCGT+CGG - chr7_4:5438459-5438478 Msa1136700:intron 40.0%
GAACCATTGTACAAAGGGAT+AGG - chr7_4:5438814-5438833 Msa1136700:intron 40.0%
TCATGGAGTTTACAGTAGGT+AGG - chr7_4:5438875-5438894 Msa1136700:intron 40.0%
AACTCTGTCAGCAGGATTAT+TGG - chr7_4:5439063-5439082 Msa1136700:CDS 40.0%
! TTTTCTTCAGTCTCATCACG+TGG - chr7_4:5439128-5439147 Msa1136700:intron 40.0%
! CGAATTGGTGATCCTCATTA+CGG - chr7_4:5439181-5439200 Msa1136700:intron 40.0%
TAGTTCCTCTTTCCGTAATG+AGG + chr7_4:5439196-5439215 None:intergenic 40.0%
GAAGCTGTTCACACAATTCT+AGG - chr7_4:5439226-5439245 Msa1136700:intron 40.0%
TCACACGTTTGCACTTGATT+TGG - chr7_4:5439567-5439586 Msa1136700:five_prime_UTR 40.0%
GTTTCAATGACACCCTTTGT+GGG - chr7_4:5439829-5439848 Msa1136700:five_prime_UTR 40.0%
GTACTGAACTATCCCACAAA+GGG + chr7_4:5439844-5439863 None:intergenic 40.0%
TGTACTGAACTATCCCACAA+AGG + chr7_4:5439845-5439864 None:intergenic 40.0%
GCAATAGGATACACAATTGC+GGG - chr7_4:5439882-5439901 Msa1136700:five_prime_UTR 40.0%
! GTTTGGGGTTGTTTGTAGAG+AGG + chr7_4:5438290-5438309 None:intergenic 45.0%
!! AGGCTAGTTTGGATTTGAGG+AGG + chr7_4:5438330-5438349 None:intergenic 45.0%
CAACATGTAGATCCTTTGGC+TGG - chr7_4:5438351-5438370 Msa1136700:intron 45.0%
AAGTACGACGAGAAGAACGA+GGG + chr7_4:5438411-5438430 None:intergenic 45.0%
!! TTTCTGGAACCGAATCAAGC+AGG - chr7_4:5438510-5438529 Msa1136700:intron 45.0%
TCTCTTGCTCCTGCTTGATT+CGG + chr7_4:5438522-5438541 None:intergenic 45.0%
CGTTCTTCTTCCTCACGAAT+CGG + chr7_4:5438615-5438634 None:intergenic 45.0%
CGATTCGTGAGGAAGAAGAA+CGG - chr7_4:5438613-5438632 Msa1136700:intron 45.0%
GTCGTCATGGAGTTTACAGT+AGG - chr7_4:5438871-5438890 Msa1136700:intron 45.0%
TGGTCATAGCTCAGTTGGTT+TGG - chr7_4:5439083-5439102 Msa1136700:CDS 45.0%
! GGTCATAGCTCAGTTGGTTT+GGG - chr7_4:5439084-5439103 Msa1136700:CDS 45.0%
!! GCTCAGTTGGTTTGGGTAAT+TGG - chr7_4:5439091-5439110 Msa1136700:intron 45.0%
GTGATCCTCATTACGGAAAG+AGG - chr7_4:5439188-5439207 Msa1136700:intron 45.0%
GGTTTCAATGACACCCTTTG+TGG - chr7_4:5439828-5439847 Msa1136700:five_prime_UTR 45.0%
! GTTTGGGGTTGTTTGTAGAG+AGG + chr7_4:5438290-5438309 None:intergenic 45.0%
!! AGGCTAGTTTGGATTTGAGG+AGG + chr7_4:5438330-5438349 None:intergenic 45.0%
CAACATGTAGATCCTTTGGC+TGG - chr7_4:5438351-5438370 Msa1136700:intron 45.0%
AAGTACGACGAGAAGAACGA+GGG + chr7_4:5438411-5438430 None:intergenic 45.0%
!! TTTCTGGAACCGAATCAAGC+AGG - chr7_4:5438510-5438529 Msa1136700:intron 45.0%
TCTCTTGCTCCTGCTTGATT+CGG + chr7_4:5438522-5438541 None:intergenic 45.0%
CGTTCTTCTTCCTCACGAAT+CGG + chr7_4:5438615-5438634 None:intergenic 45.0%
CGATTCGTGAGGAAGAAGAA+CGG - chr7_4:5438613-5438632 Msa1136700:intron 45.0%
GTCGTCATGGAGTTTACAGT+AGG - chr7_4:5438871-5438890 Msa1136700:intron 45.0%
TGGTCATAGCTCAGTTGGTT+TGG - chr7_4:5439083-5439102 Msa1136700:CDS 45.0%
! GGTCATAGCTCAGTTGGTTT+GGG - chr7_4:5439084-5439103 Msa1136700:CDS 45.0%
!! GCTCAGTTGGTTTGGGTAAT+TGG - chr7_4:5439091-5439110 Msa1136700:intron 45.0%
GTGATCCTCATTACGGAAAG+AGG - chr7_4:5439188-5439207 Msa1136700:intron 45.0%
GGTTTCAATGACACCCTTTG+TGG - chr7_4:5439828-5439847 Msa1136700:five_prime_UTR 45.0%
GGTTGTTTGTAGAGAGGAGG+AGG + chr7_4:5438284-5438303 None:intergenic 50.0%
TGGGGTTGTTTGTAGAGAGG+AGG + chr7_4:5438287-5438306 None:intergenic 50.0%
CATGTAGATCCTTTGGCTGG+TGG - chr7_4:5438354-5438373 Msa1136700:intron 50.0%
AGTACGACGAGAAGAACGAG+GGG + chr7_4:5438410-5438429 None:intergenic 50.0%
GAAGTACGACGAGAAGAACG+AGG + chr7_4:5438412-5438431 None:intergenic 50.0%
! CTGACGGAGAGAAGTTGATC+TGG + chr7_4:5438437-5438456 None:intergenic 50.0%
TTTGCGGTTTCGTCGGATCT+TGG - chr7_4:5438466-5438485 Msa1136700:intron 50.0%
TTGCGGTTTCGTCGGATCTT+GGG - chr7_4:5438467-5438486 Msa1136700:intron 50.0%
GTTTCGTCGGATCTTGGGTT+TGG - chr7_4:5438472-5438491 Msa1136700:intron 50.0%
!! GCTGTCTGTGTGTTGGTTTC+TGG - chr7_4:5438494-5438513 Msa1136700:intron 50.0%
GAATCAAGCAGGAGCAAGAG+AGG - chr7_4:5438521-5438540 Msa1136700:intron 50.0%
TTCTTCCTCACGAATCGGTC+GGG + chr7_4:5438610-5438629 None:intergenic 50.0%
CTTCTTCCTCACGAATCGGT+CGG + chr7_4:5438611-5438630 None:intergenic 50.0%
TGTTGTGGCTGTCATGACTG+TGG - chr7_4:5438846-5438865 Msa1136700:intron 50.0%
CATGACTGTGGTTGTCGTCA+TGG - chr7_4:5438858-5438877 Msa1136700:intron 50.0%
GCAGGAGGAACCTTATGCAA+AGG - chr7_4:5439022-5439041 Msa1136700:CDS 50.0%
GGATTAGGAACTCTGTCAGC+AGG - chr7_4:5439055-5439074 Msa1136700:CDS 50.0%
GGTTGTTTGTAGAGAGGAGG+AGG + chr7_4:5438284-5438303 None:intergenic 50.0%
TGGGGTTGTTTGTAGAGAGG+AGG + chr7_4:5438287-5438306 None:intergenic 50.0%
CATGTAGATCCTTTGGCTGG+TGG - chr7_4:5438354-5438373 Msa1136700:intron 50.0%
AGTACGACGAGAAGAACGAG+GGG + chr7_4:5438410-5438429 None:intergenic 50.0%
GAAGTACGACGAGAAGAACG+AGG + chr7_4:5438412-5438431 None:intergenic 50.0%
! CTGACGGAGAGAAGTTGATC+TGG + chr7_4:5438437-5438456 None:intergenic 50.0%
TTTGCGGTTTCGTCGGATCT+TGG - chr7_4:5438466-5438485 Msa1136700:intron 50.0%
TTGCGGTTTCGTCGGATCTT+GGG - chr7_4:5438467-5438486 Msa1136700:intron 50.0%
GTTTCGTCGGATCTTGGGTT+TGG - chr7_4:5438472-5438491 Msa1136700:intron 50.0%
!! GCTGTCTGTGTGTTGGTTTC+TGG - chr7_4:5438494-5438513 Msa1136700:intron 50.0%
GAATCAAGCAGGAGCAAGAG+AGG - chr7_4:5438521-5438540 Msa1136700:intron 50.0%
TTCTTCCTCACGAATCGGTC+GGG + chr7_4:5438610-5438629 None:intergenic 50.0%
CTTCTTCCTCACGAATCGGT+CGG + chr7_4:5438611-5438630 None:intergenic 50.0%
TGTTGTGGCTGTCATGACTG+TGG - chr7_4:5438846-5438865 Msa1136700:intron 50.0%
CATGACTGTGGTTGTCGTCA+TGG - chr7_4:5438858-5438877 Msa1136700:intron 50.0%
GCAGGAGGAACCTTATGCAA+AGG - chr7_4:5439022-5439041 Msa1136700:CDS 50.0%
GGATTAGGAACTCTGTCAGC+AGG - chr7_4:5439055-5439074 Msa1136700:CDS 50.0%
GGGTTTGGCTGTCTGTGTGT+TGG - chr7_4:5438487-5438506 Msa1136700:intron 55.0%
AGCAGGAGCAAGAGAGGAAG+TGG - chr7_4:5438527-5438546 Msa1136700:intron 55.0%
!! TAGATCCCGACCGATTCGTG+AGG - chr7_4:5438602-5438621 Msa1136700:intron 55.0%
GGGTTTGGCTGTCTGTGTGT+TGG - chr7_4:5438487-5438506 Msa1136700:intron 55.0%
AGCAGGAGCAAGAGAGGAAG+TGG - chr7_4:5438527-5438546 Msa1136700:intron 55.0%
!! TAGATCCCGACCGATTCGTG+AGG - chr7_4:5438602-5438621 Msa1136700:intron 55.0%
! TCCTTTGGCTGGTGGTGCGA+TGG - chr7_4:5438362-5438381 Msa1136700:intron 60.0%
! CCTTTGGCTGGTGGTGCGAT+GGG - chr7_4:5438363-5438382 Msa1136700:intron 60.0%
! TCCTTTGGCTGGTGGTGCGA+TGG - chr7_4:5438362-5438381 Msa1136700:intron 60.0%
! CCTTTGGCTGGTGGTGCGAT+GGG - chr7_4:5438363-5438382 Msa1136700:intron 60.0%
CCCATCGCACCACCAGCCAA+AGG + chr7_4:5438366-5438385 None:intergenic 65.0%
!! AGAAGAACGAGGGGCGCTGG+TGG + chr7_4:5438401-5438420 None:intergenic 65.0%
! ACGAGAAGAACGAGGGGCGC+TGG + chr7_4:5438404-5438423 None:intergenic 65.0%
CCCATCGCACCACCAGCCAA+AGG + chr7_4:5438366-5438385 None:intergenic 65.0%
!! AGAAGAACGAGGGGCGCTGG+TGG + chr7_4:5438401-5438420 None:intergenic 65.0%
! ACGAGAAGAACGAGGGGCGC+TGG + chr7_4:5438404-5438423 None:intergenic 65.0%
!! AACGAGGGGCGCTGGTGGTG+CGG + chr7_4:5438396-5438415 None:intergenic 70.0%
!! AACGAGGGGCGCTGGTGGTG+CGG + chr7_4:5438396-5438415 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 5438212 5439923 5438212 ID=Msa1136700;Name=Msa1136700
chr7_4 mRNA 5438212 5439923 5438212 ID=Msa1136700-mRNA-1;Parent=Msa1136700;Name=Msa1136700-mRNA-1;_AED=0.14;_eAED=0.14;_QI=599|1|0.66|1|1|1|3|0|133
chr7_4 exon 5438212 5438309 5438212 ID=Msa1136700-mRNA-1:exon:1419;Parent=Msa1136700-mRNA-1
chr7_4 exon 5438888 5439109 5438888 ID=Msa1136700-mRNA-1:exon:1418;Parent=Msa1136700-mRNA-1
chr7_4 exon 5439243 5439923 5439243 ID=Msa1136700-mRNA-1:exon:1417;Parent=Msa1136700-mRNA-1
chr7_4 five_prime_UTR 5439325 5439923 5439325 ID=Msa1136700-mRNA-1:five_prime_utr;Parent=Msa1136700-mRNA-1
chr7_4 CDS 5439243 5439324 5439243 ID=Msa1136700-mRNA-1:cds;Parent=Msa1136700-mRNA-1
chr7_4 CDS 5438888 5439109 5438888 ID=Msa1136700-mRNA-1:cds;Parent=Msa1136700-mRNA-1
chr7_4 CDS 5438212 5438309 5438212 ID=Msa1136700-mRNA-1:cds;Parent=Msa1136700-mRNA-1
Gene Sequence

>Msa1136700

ATGGAACCATTGTACAAAGGGATAGGAAAAAATGCTGTTGTGGCTGTCATGACTGTGGTTGTCGTCATGGAGTTTACAGTAGGAGGAACCTTATGCAAAGGACTTAATAGAGGATTAGGAACTCTGTCAGCAGGATTATTGGTCATAGCTCAGTTGGTTTGGGTAATTGGTCCTTCTGTTATGATTTTCTTCAGTCTCATCACGTGGTATACTTCATCACTTTTAGCTGAATGTTATCGAATTGGTGATCCTCATTACGGAAAGAGGAACTATACTTTCATGGAAGCTGTTCACACAATTCTAGGAGGTTTCAATGACACCCTTTGTGGGATAGTTCAGTACACAAATCTTTACGGAACTGCAATAGGATACACAATTGCGGGTGCCATTAGCATGATGTGA
Protein sequence

>Msa1136700

MEPLYKGIGKNAVVAVMTVVVVMEFTVGGTLCKGLNRGLGTLSAGLLVIAQLVWVIGPSVMIFFSLITWYTSSLLAECYRIGDPHYGKRNYTFMEAVHTILGGFNDTLCGIVQYTNLYGTAIGYTIAGAISMM*