Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142540 | XP_013447886.1 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 1.44e-89 | 266 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142540 | A0A072TYJ6 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 6.90e-90 | 266 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1095430 | Msa1142540 | 0.800437 | 1.441604e-48 | -8.615850e-47 |
| Msa1095440 | Msa1142540 | 0.801267 | 9.760017e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142540 | MtrunA17_Chr7g0223391 | 95.620 | 137 | 6 | 0 | 1 | 137 | 18 | 154 | 2.85e-93 | 266 |
| Msa1142540 | MtrunA17_Chr7g0223401 | 89.781 | 137 | 14 | 0 | 1 | 137 | 6 | 142 | 9.28e-88 | 251 |
| Msa1142540 | MtrunA17_Chr8g0356181 | 49.286 | 140 | 68 | 1 | 1 | 137 | 1 | 140 | 2.61e-42 | 136 |
| Msa1142540 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 1.19e-20 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142540 | AT2G44310.1 | 49.635 | 137 | 67 | 1 | 1 | 135 | 1 | 137 | 8.52e-42 | 135 |
Find 28 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTAAGCAGCCAATTTAC+TGG | 0.241600 | 7_4:-17032905 | None:intergenic |
| TGAAGGAGCTCAAGAGCTTT+AGG | 0.251453 | 7_4:+17032647 | Msa1142540:CDS |
| CGGTTGATCTTGAAGAATTC+AGG | 0.278025 | 7_4:+17032773 | Msa1142540:CDS |
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_4:-17032709 | None:intergenic |
| GAAGGAGCTCAAGAGCTTTA+GGG | 0.349802 | 7_4:+17032648 | Msa1142540:CDS |
| TGAAAGAGAGTCCAGTAAAT+TGG | 0.375239 | 7_4:+17032894 | Msa1142540:CDS |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_4:-17032552 | None:intergenic |
| TCTTCCAACACCATTTGAAT+TGG | 0.382295 | 7_4:-17032847 | None:intergenic |
| CAGTTTGATCACAACTTGAA+TGG | 0.425360 | 7_4:+17032748 | Msa1142540:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_4:-17032546 | None:intergenic |
| TTAATAGCTCGGTAGGAAAT+CGG | 0.454217 | 7_4:+17032572 | Msa1142540:CDS |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_4:-17032632 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_4:-17032708 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_4:+17032837 | Msa1142540:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_4:+17032630 | Msa1142540:CDS |
| CATGCCAATTCAAATGGTGT+TGG | 0.494072 | 7_4:+17032843 | Msa1142540:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_4:+17032816 | Msa1142540:CDS |
| GAAGCATTTAATAGCTCGGT+AGG | 0.541215 | 7_4:+17032565 | Msa1142540:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.545635 | 7_4:+17032811 | Msa1142540:CDS |
| TGAAGAAGCATTTAATAGCT+CGG | 0.548389 | 7_4:+17032561 | Msa1142540:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_4:+17032817 | Msa1142540:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_4:+17032678 | Msa1142540:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_4:-17032536 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_4:+17032804 | Msa1142540:CDS |
| TGATGCTTGCAATGGCAGAT+GGG | 0.619568 | 7_4:+17032812 | Msa1142540:CDS |
| TGATCACAACTTGAATGGCA+CGG | 0.635410 | 7_4:+17032753 | Msa1142540:CDS |
| TGATCTTGAAGAATTCAGGA+AGG | 0.643588 | 7_4:+17032777 | Msa1142540:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7_4:+17032601 | Msa1142540:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGTGTAGAAATTTTAGA+TGG | + | chr7_4:17032511-17032530 | Msa1142540:CDS | 25.0% |
| TAAATGCTTCTTCATCTTCA+AGG | - | chr7_4:17032555-17032574 | None:intergenic | 30.0% | |
| !! | TGAAGAAGCATTTAATAGCT+CGG | + | chr7_4:17032561-17032580 | Msa1142540:CDS | 30.0% |
| TTAATAGCTCGGTAGGAAAT+CGG | + | chr7_4:17032572-17032591 | Msa1142540:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | + | chr7_4:17032667-17032686 | Msa1142540:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | + | chr7_4:17032678-17032697 | Msa1142540:CDS | 35.0% |
| CAGTTTGATCACAACTTGAA+TGG | + | chr7_4:17032748-17032767 | Msa1142540:CDS | 35.0% | |
| TGATCTTGAAGAATTCAGGA+AGG | + | chr7_4:17032777-17032796 | Msa1142540:CDS | 35.0% | |
| TAAGCAAATGATGCTTGCAA+TGG | + | chr7_4:17032804-17032823 | Msa1142540:CDS | 35.0% | |
| TCTTCCAACACCATTTGAAT+TGG | - | chr7_4:17032850-17032869 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTC+AGG | - | chr7_4:17032882-17032901 | None:intergenic | 35.0% |
| TGAAAGAGAGTCCAGTAAAT+TGG | + | chr7_4:17032894-17032913 | Msa1142540:CDS | 35.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | - | chr7_4:17032539-17032558 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | - | chr7_4:17032549-17032568 | None:intergenic | 40.0% | |
| ! | GAAGCATTTAATAGCTCGGT+AGG | + | chr7_4:17032565-17032584 | Msa1142540:CDS | 40.0% |
| CTCCTATGATGAGATGTTGA+AGG | + | chr7_4:17032630-17032649 | Msa1142540:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | - | chr7_4:17032635-17032654 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | - | chr7_4:17032711-17032730 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | - | chr7_4:17032712-17032731 | None:intergenic | 40.0% | |
| TGATCACAACTTGAATGGCA+CGG | + | chr7_4:17032753-17032772 | Msa1142540:CDS | 40.0% | |
| CGGTTGATCTTGAAGAATTC+AGG | + | chr7_4:17032773-17032792 | Msa1142540:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | + | chr7_4:17032837-17032856 | Msa1142540:CDS | 40.0% | |
| CATGCCAATTCAAATGGTGT+TGG | + | chr7_4:17032843-17032862 | Msa1142540:CDS | 40.0% | |
| TGCCTCGACACAAATAACGA+CGG | + | chr7_4:17032601-17032620 | Msa1142540:CDS | 45.0% | |
| TGAAGGAGCTCAAGAGCTTT+AGG | + | chr7_4:17032647-17032666 | Msa1142540:CDS | 45.0% | |
| GAAGGAGCTCAAGAGCTTTA+GGG | + | chr7_4:17032648-17032667 | Msa1142540:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7_4:17032811-17032830 | Msa1142540:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | + | chr7_4:17032812-17032831 | Msa1142540:CDS | 45.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | - | chr7_4:17032606-17032625 | None:intergenic | 50.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | + | chr7_4:17032817-17032836 | Msa1142540:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | + | chr7_4:17032816-17032835 | Msa1142540:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 17032511 | 17032924 | 17032511 | ID=Msa1142540;Name=Msa1142540 |
| chr7_4 | mRNA | 17032511 | 17032924 | 17032511 | ID=Msa1142540-mRNA-1;Parent=Msa1142540;Name=Msa1142540-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_4 | exon | 17032511 | 17032924 | 17032511 | ID=Msa1142540-mRNA-1:exon:3817;Parent=Msa1142540-mRNA-1 |
| chr7_4 | CDS | 17032511 | 17032924 | 17032511 | ID=Msa1142540-mRNA-1:cds;Parent=Msa1142540-mRNA-1 |
| Gene Sequence |
| Protein sequence |
