Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095440 | XP_013447886.1 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 2.26e-89 | 266 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095440 | A0A072TYJ6 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 1.08e-89 | 266 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011860 | Msa1095440 | 0.818516 | 1.881836e-52 | -8.615850e-47 |
| Msa1053310 | Msa1095440 | 0.813764 | 2.169477e-51 | -8.615850e-47 |
| Msa1053330 | Msa1095440 | 0.845235 | 4.608961e-59 | -8.615850e-47 |
| Msa1053340 | Msa1095440 | 0.804995 | 1.652514e-49 | -8.615850e-47 |
| Msa1095430 | Msa1095440 | 0.946458 | 4.438682e-105 | -8.615850e-47 |
| Msa1095440 | Msa1142540 | 0.801267 | 9.760017e-49 | -8.615850e-47 |
| Msa1095440 | Msa1142590 | 0.805395 | 1.362816e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095440 | MtrunA17_Chr7g0223391 | 95.620 | 137 | 6 | 0 | 1 | 137 | 18 | 154 | 3.77e-93 | 266 |
| Msa1095440 | MtrunA17_Chr7g0223401 | 89.781 | 137 | 14 | 0 | 1 | 137 | 6 | 142 | 1.08e-87 | 251 |
| Msa1095440 | MtrunA17_Chr8g0356181 | 48.227 | 141 | 70 | 1 | 1 | 138 | 1 | 141 | 6.40e-42 | 135 |
| Msa1095440 | MtrunA17_Chr1g0190921 | 35.115 | 131 | 80 | 1 | 1 | 131 | 1 | 126 | 8.13e-19 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095440 | AT2G44310.1 | 48.905 | 137 | 68 | 1 | 1 | 135 | 1 | 137 | 3.68e-41 | 134 |
Find 31 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_3:+18301980 | None:intergenic |
| CATTAAAGCAGCCAATTTAC+TGG | 0.266144 | 7_3:-18302378 | None:intergenic |
| CGGTTGATCTTGAAGAATTC+AGG | 0.278025 | 7_3:+18302246 | Msa1095440:CDS |
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_3:-18302182 | None:intergenic |
| TGAAAGAGAGTCCAGTAAAT+TGG | 0.375239 | 7_3:+18302367 | Msa1095440:CDS |
| ATTGGCTGCTTTAATGCTAC+TGG | 0.376171 | 7_3:+18302385 | Msa1095440:CDS |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_3:-18302025 | None:intergenic |
| TCTTCCAACACCATTTGAAT+TGG | 0.382295 | 7_3:-18302320 | None:intergenic |
| CAGTTTGATCACAACTTGAA+TGG | 0.425360 | 7_3:+18302221 | Msa1095440:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_3:-18302019 | None:intergenic |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_3:-18302105 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_3:-18302181 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_3:+18302310 | Msa1095440:CDS |
| TTAATAGCTCGGTAGGAAAT+CGG | 0.459506 | 7_3:+18302045 | Msa1095440:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_3:+18302103 | Msa1095440:CDS |
| CATGCCAATTCAAATGGTGT+TGG | 0.494072 | 7_3:+18302316 | Msa1095440:CDS |
| GAAGGAGCTCAAGAGCTTGA+GGG | 0.520375 | 7_3:+18302121 | Msa1095440:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_3:+18302289 | Msa1095440:CDS |
| GAAGCATTTAATAGCTCGGT+AGG | 0.541215 | 7_3:+18302038 | Msa1095440:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.545635 | 7_3:+18302284 | Msa1095440:CDS |
| TGAAGAAGCATTTAATAGCT+CGG | 0.548389 | 7_3:+18302034 | Msa1095440:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_3:+18302290 | Msa1095440:CDS |
| TGAAGGAGCTCAAGAGCTTG+AGG | 0.558625 | 7_3:+18302120 | Msa1095440:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_3:+18302151 | Msa1095440:CDS |
| TTGGCTGCTTTAATGCTACT+GGG | 0.591273 | 7_3:+18302386 | Msa1095440:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_3:-18302009 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_3:+18302277 | Msa1095440:CDS |
| TGATGCTTGCAATGGCAGAT+GGG | 0.619568 | 7_3:+18302285 | Msa1095440:CDS |
| TGATCACAACTTGAATGGCA+CGG | 0.635410 | 7_3:+18302226 | Msa1095440:CDS |
| TGATCTTGAAGAATTCAGGA+AGG | 0.643588 | 7_3:+18302250 | Msa1095440:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7_3:+18302074 | Msa1095440:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | + | chr7_3:18301984-18302003 | Msa1095440:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | - | chr7_3:18302028-18302047 | None:intergenic | 30.0% | |
| !! | TGAAGAAGCATTTAATAGCT+CGG | + | chr7_3:18302034-18302053 | Msa1095440:CDS | 30.0% |
| TTAATAGCTCGGTAGGAAAT+CGG | + | chr7_3:18302045-18302064 | Msa1095440:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | + | chr7_3:18302140-18302159 | Msa1095440:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | + | chr7_3:18302151-18302170 | Msa1095440:CDS | 35.0% |
| CAGTTTGATCACAACTTGAA+TGG | + | chr7_3:18302221-18302240 | Msa1095440:CDS | 35.0% | |
| TGATCTTGAAGAATTCAGGA+AGG | + | chr7_3:18302250-18302269 | Msa1095440:CDS | 35.0% | |
| TAAGCAAATGATGCTTGCAA+TGG | + | chr7_3:18302277-18302296 | Msa1095440:CDS | 35.0% | |
| TCTTCCAACACCATTTGAAT+TGG | - | chr7_3:18302323-18302342 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTC+AGG | - | chr7_3:18302355-18302374 | None:intergenic | 35.0% |
| TGAAAGAGAGTCCAGTAAAT+TGG | + | chr7_3:18302367-18302386 | Msa1095440:CDS | 35.0% | |
| CATTAAAGCAGCCAATTTAC+TGG | - | chr7_3:18302381-18302400 | None:intergenic | 35.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | - | chr7_3:18302012-18302031 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | - | chr7_3:18302022-18302041 | None:intergenic | 40.0% | |
| ! | GAAGCATTTAATAGCTCGGT+AGG | + | chr7_3:18302038-18302057 | Msa1095440:CDS | 40.0% |
| CTCCTATGATGAGATGTTGA+AGG | + | chr7_3:18302103-18302122 | Msa1095440:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | - | chr7_3:18302108-18302127 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | - | chr7_3:18302184-18302203 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | - | chr7_3:18302185-18302204 | None:intergenic | 40.0% | |
| TGATCACAACTTGAATGGCA+CGG | + | chr7_3:18302226-18302245 | Msa1095440:CDS | 40.0% | |
| CGGTTGATCTTGAAGAATTC+AGG | + | chr7_3:18302246-18302265 | Msa1095440:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | + | chr7_3:18302310-18302329 | Msa1095440:CDS | 40.0% | |
| CATGCCAATTCAAATGGTGT+TGG | + | chr7_3:18302316-18302335 | Msa1095440:CDS | 40.0% | |
| ! | ATTGGCTGCTTTAATGCTAC+TGG | + | chr7_3:18302385-18302404 | Msa1095440:CDS | 40.0% |
| ! | TTGGCTGCTTTAATGCTACT+GGG | + | chr7_3:18302386-18302405 | Msa1095440:CDS | 40.0% |
| TGCCTCGACACAAATAACGA+CGG | + | chr7_3:18302074-18302093 | Msa1095440:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7_3:18302284-18302303 | Msa1095440:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | + | chr7_3:18302285-18302304 | Msa1095440:CDS | 45.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | - | chr7_3:18302079-18302098 | None:intergenic | 50.0% | |
| TGAAGGAGCTCAAGAGCTTG+AGG | + | chr7_3:18302120-18302139 | Msa1095440:CDS | 50.0% | |
| GAAGGAGCTCAAGAGCTTGA+GGG | + | chr7_3:18302121-18302140 | Msa1095440:CDS | 50.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | + | chr7_3:18302290-18302309 | Msa1095440:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | + | chr7_3:18302289-18302308 | Msa1095440:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 18301984 | 18302412 | 18301984 | ID=Msa1095440;Name=Msa1095440 |
| chr7_3 | mRNA | 18301984 | 18302412 | 18301984 | ID=Msa1095440-mRNA-1;Parent=Msa1095440;Name=Msa1095440-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|142 |
| chr7_3 | exon | 18301984 | 18302412 | 18301984 | ID=Msa1095440-mRNA-1:exon:3972;Parent=Msa1095440-mRNA-1 |
| chr7_3 | CDS | 18301984 | 18302412 | 18301984 | ID=Msa1095440-mRNA-1:cds;Parent=Msa1095440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
