Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095430 | XP_013447886.1 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 2.26e-89 | 266 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095430 | A0A072TYJ6 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 1.08e-89 | 266 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011800 | Msa1095430 | 0.806183 | 9.307436e-50 | -8.615850e-47 |
| Msa1053310 | Msa1095430 | 0.802542 | 5.338770e-49 | -8.615850e-47 |
| Msa1053330 | Msa1095430 | 0.835019 | 2.140126e-56 | -8.615850e-47 |
| Msa1053340 | Msa1095430 | 0.802071 | 6.676890e-49 | -8.615850e-47 |
| Msa1095430 | Msa1095440 | 0.946458 | 4.438682e-105 | -8.615850e-47 |
| Msa1095430 | Msa1142540 | 0.800437 | 1.441604e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095430 | MtrunA17_Chr7g0223391 | 95.620 | 137 | 6 | 0 | 1 | 137 | 18 | 154 | 3.77e-93 | 266 |
| Msa1095430 | MtrunA17_Chr7g0223401 | 89.781 | 137 | 14 | 0 | 1 | 137 | 6 | 142 | 1.08e-87 | 251 |
| Msa1095430 | MtrunA17_Chr8g0356181 | 48.227 | 141 | 70 | 1 | 1 | 138 | 1 | 141 | 6.40e-42 | 135 |
| Msa1095430 | MtrunA17_Chr1g0190921 | 35.115 | 131 | 80 | 1 | 1 | 131 | 1 | 126 | 8.13e-19 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095430 | AT2G44310.1 | 48.905 | 137 | 68 | 1 | 1 | 135 | 1 | 137 | 3.68e-41 | 134 |
Find 31 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_3:+18298518 | None:intergenic |
| CATTAAAGCAGCCAATTTAC+TGG | 0.266144 | 7_3:-18298916 | None:intergenic |
| CGGTTGATCTTGAAGAATTC+AGG | 0.278025 | 7_3:+18298784 | Msa1095430:CDS |
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_3:-18298720 | None:intergenic |
| TGAAAGAGAGTCCAGTAAAT+TGG | 0.375239 | 7_3:+18298905 | Msa1095430:CDS |
| ATTGGCTGCTTTAATGCTAC+TGG | 0.376171 | 7_3:+18298923 | Msa1095430:CDS |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_3:-18298563 | None:intergenic |
| TCTTCCAACACCATTTGAAT+TGG | 0.382295 | 7_3:-18298858 | None:intergenic |
| CAGTTTGATCACAACTTGAA+TGG | 0.425360 | 7_3:+18298759 | Msa1095430:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_3:-18298557 | None:intergenic |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_3:-18298643 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_3:-18298719 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_3:+18298848 | Msa1095430:CDS |
| TTAATAGCTCGGTAGGAAAT+CGG | 0.459506 | 7_3:+18298583 | Msa1095430:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_3:+18298641 | Msa1095430:CDS |
| CATGCCAATTCAAATGGTGT+TGG | 0.494072 | 7_3:+18298854 | Msa1095430:CDS |
| GAAGGAGCTCAAGAGCTTGA+GGG | 0.520375 | 7_3:+18298659 | Msa1095430:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_3:+18298827 | Msa1095430:CDS |
| GAAGCATTTAATAGCTCGGT+AGG | 0.541215 | 7_3:+18298576 | Msa1095430:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.545635 | 7_3:+18298822 | Msa1095430:CDS |
| TGAAGAAGCATTTAATAGCT+CGG | 0.548389 | 7_3:+18298572 | Msa1095430:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_3:+18298828 | Msa1095430:CDS |
| TGAAGGAGCTCAAGAGCTTG+AGG | 0.558625 | 7_3:+18298658 | Msa1095430:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_3:+18298689 | Msa1095430:CDS |
| TTGGCTGCTTTAATGCTACT+GGG | 0.591273 | 7_3:+18298924 | Msa1095430:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_3:-18298547 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_3:+18298815 | Msa1095430:CDS |
| TGATGCTTGCAATGGCAGAT+GGG | 0.619568 | 7_3:+18298823 | Msa1095430:CDS |
| TGATCACAACTTGAATGGCA+CGG | 0.635410 | 7_3:+18298764 | Msa1095430:CDS |
| TGATCTTGAAGAATTCAGGA+AGG | 0.643588 | 7_3:+18298788 | Msa1095430:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7_3:+18298612 | Msa1095430:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | + | chr7_3:18298522-18298541 | Msa1095430:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | - | chr7_3:18298566-18298585 | None:intergenic | 30.0% | |
| !! | TGAAGAAGCATTTAATAGCT+CGG | + | chr7_3:18298572-18298591 | Msa1095430:CDS | 30.0% |
| TTAATAGCTCGGTAGGAAAT+CGG | + | chr7_3:18298583-18298602 | Msa1095430:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | + | chr7_3:18298678-18298697 | Msa1095430:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | + | chr7_3:18298689-18298708 | Msa1095430:CDS | 35.0% |
| CAGTTTGATCACAACTTGAA+TGG | + | chr7_3:18298759-18298778 | Msa1095430:CDS | 35.0% | |
| TGATCTTGAAGAATTCAGGA+AGG | + | chr7_3:18298788-18298807 | Msa1095430:CDS | 35.0% | |
| TAAGCAAATGATGCTTGCAA+TGG | + | chr7_3:18298815-18298834 | Msa1095430:CDS | 35.0% | |
| TCTTCCAACACCATTTGAAT+TGG | - | chr7_3:18298861-18298880 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTC+AGG | - | chr7_3:18298893-18298912 | None:intergenic | 35.0% |
| TGAAAGAGAGTCCAGTAAAT+TGG | + | chr7_3:18298905-18298924 | Msa1095430:CDS | 35.0% | |
| CATTAAAGCAGCCAATTTAC+TGG | - | chr7_3:18298919-18298938 | None:intergenic | 35.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | - | chr7_3:18298550-18298569 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | - | chr7_3:18298560-18298579 | None:intergenic | 40.0% | |
| ! | GAAGCATTTAATAGCTCGGT+AGG | + | chr7_3:18298576-18298595 | Msa1095430:CDS | 40.0% |
| CTCCTATGATGAGATGTTGA+AGG | + | chr7_3:18298641-18298660 | Msa1095430:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | - | chr7_3:18298646-18298665 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | - | chr7_3:18298722-18298741 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | - | chr7_3:18298723-18298742 | None:intergenic | 40.0% | |
| TGATCACAACTTGAATGGCA+CGG | + | chr7_3:18298764-18298783 | Msa1095430:CDS | 40.0% | |
| CGGTTGATCTTGAAGAATTC+AGG | + | chr7_3:18298784-18298803 | Msa1095430:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | + | chr7_3:18298848-18298867 | Msa1095430:CDS | 40.0% | |
| CATGCCAATTCAAATGGTGT+TGG | + | chr7_3:18298854-18298873 | Msa1095430:CDS | 40.0% | |
| ! | ATTGGCTGCTTTAATGCTAC+TGG | + | chr7_3:18298923-18298942 | Msa1095430:CDS | 40.0% |
| ! | TTGGCTGCTTTAATGCTACT+GGG | + | chr7_3:18298924-18298943 | Msa1095430:CDS | 40.0% |
| TGCCTCGACACAAATAACGA+CGG | + | chr7_3:18298612-18298631 | Msa1095430:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7_3:18298822-18298841 | Msa1095430:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | + | chr7_3:18298823-18298842 | Msa1095430:CDS | 45.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | - | chr7_3:18298617-18298636 | None:intergenic | 50.0% | |
| TGAAGGAGCTCAAGAGCTTG+AGG | + | chr7_3:18298658-18298677 | Msa1095430:CDS | 50.0% | |
| GAAGGAGCTCAAGAGCTTGA+GGG | + | chr7_3:18298659-18298678 | Msa1095430:CDS | 50.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | + | chr7_3:18298828-18298847 | Msa1095430:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | + | chr7_3:18298827-18298846 | Msa1095430:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 18298522 | 18298950 | 18298522 | ID=Msa1095430;Name=Msa1095430 |
| chr7_3 | mRNA | 18298522 | 18298950 | 18298522 | ID=Msa1095430-mRNA-1;Parent=Msa1095430;Name=Msa1095430-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|142 |
| chr7_3 | exon | 18298522 | 18298950 | 18298522 | ID=Msa1095430-mRNA-1:exon:3971;Parent=Msa1095430-mRNA-1 |
| chr7_3 | CDS | 18298522 | 18298950 | 18298522 | ID=Msa1095430-mRNA-1:cds;Parent=Msa1095430-mRNA-1 |
| Gene Sequence |
| Protein sequence |
