AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1144820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1144820 MtrunA17_Chr8g0392751 94.328 335 13 4 1 329 1 335 0.0 629
Msa1144820 MtrunA17_Chr6g0482961 64.744 312 97 5 1 305 3 308 2.03e-133 383
Msa1144820 MtrunA17_Chr4g0022791 38.614 303 149 10 34 320 28 309 3.26e-60 195
Msa1144820 MtrunA17_Chr2g0323391 38.284 303 152 8 39 329 19 298 1.58e-57 188
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1144820 AT3G18050.1 55.060 336 113 10 12 329 11 326 7.70e-108 318
Msa1144820 AT4G28100.1 41.328 271 129 6 33 296 26 273 1.08e-61 199

Find 88 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATGAGAGATTCTATTAA+AGG 0.187880 7_4:+21325956 None:intergenic
AGCAGCATGCTTGTTGGATT+TGG 0.241303 7_4:+21328147 None:intergenic
TGTTCATCTGAGAGACTTAA+AGG 0.251190 7_4:+21328199 None:intergenic
GGAAAGGCTTTGAAAGTTAG+AGG 0.261782 7_4:-21327962 Msa1144820:CDS
TAACAAAGATTGTGGACTAA+TGG 0.300105 7_4:-21326433 Msa1144820:CDS
ACAATCTTTGTTATGGATCT+TGG 0.301361 7_4:+21326443 None:intergenic
GTCAATGGATTTCCTGGTCT+TGG 0.301632 7_4:-21327767 Msa1144820:CDS
AGAAACATGTGAGAGTGATT+TGG 0.318675 7_4:-21327984 Msa1144820:CDS
TCATTTAAACTTAATCATAA+TGG 0.319467 7_4:-21327854 Msa1144820:CDS
CTTACATTCACACGGTTACT+TGG 0.323135 7_4:-21326377 Msa1144820:CDS
GAATGAACAAGATCAGAATA+AGG 0.327555 7_4:+21328344 None:intergenic
TTTATTGCTATTGTGGTATT+AGG 0.328867 7_4:-21327901 Msa1144820:CDS
TTTAGTTATTATTTGAAATT+GGG 0.332077 7_4:-21326064 Msa1144820:three_prime_UTR
GAAACATGTGAGAGTGATTT+GGG 0.335585 7_4:-21327983 Msa1144820:CDS
ATCATAATGGAAATCTTGTT+GGG 0.350569 7_4:-21327841 Msa1144820:CDS
AATCATGTCAATGGATTTCC+TGG 0.353522 7_4:-21327773 Msa1144820:CDS
CACTTAACTCAAAACTCAAA+TGG 0.364950 7_4:-21326169 Msa1144820:exon
TATTAGTCTATCGCAGAATC+TGG 0.368092 7_4:-21325986 Msa1144820:three_prime_UTR
TTAGTCCACAATCTTTGTTA+TGG 0.370896 7_4:+21326436 None:intergenic
GCTTACTCTGCCACTGCTCT+TGG 0.375981 7_4:-21328064 Msa1144820:CDS
AACAAAGATTGTGGACTAAT+GGG 0.379439 7_4:-21326432 Msa1144820:CDS
CTTGCAACATCAGCTTGTTC+AGG 0.386076 7_4:+21328226 None:intergenic
AATCATAATGGAAATCTTGT+TGG 0.388039 7_4:-21327842 Msa1144820:CDS
GGGATTGTTCCTTGTGAAGA+TGG 0.393542 7_4:+21328256 None:intergenic
ACTGTTGCTGCATCAGGTAT+AGG 0.404077 7_4:+21328298 None:intergenic
GTTCCTTGTGAAGATGGATT+TGG 0.404459 7_4:+21328262 None:intergenic
CTGTTGCTGCATCAGGTATA+GGG 0.413282 7_4:+21328299 None:intergenic
AAATATACAAATGCCAAGAT+AGG 0.414288 7_4:+21326128 None:intergenic
AAGTAAAGATTGGTGCTTTG+AGG 0.419197 7_4:+21326232 None:intergenic
CATGGCTTCTTGCTAAGAAT+AGG 0.421289 7_4:-21326404 Msa1144820:CDS
GTATATTTGAATATTAGCTT+TGG 0.421378 7_4:-21326113 Msa1144820:three_prime_UTR
ATCATCAGCAGCATGCTTGT+TGG 0.425684 7_4:+21328141 None:intergenic
GTGCTTGCATAGCCTCTATT+CGG 0.425995 7_4:-21327732 Msa1144820:intron
AAAAGATTGATCTCCTATCT+TGG 0.436673 7_4:-21326141 Msa1144820:three_prime_UTR
AGTAGCAGCAATCATGTCAA+TGG 0.437121 7_4:-21327782 Msa1144820:CDS
TACTCTGCCACTGCTCTTGG+TGG 0.440680 7_4:-21328061 Msa1144820:CDS
GGTTGAACTGTTGCTGCATC+AGG 0.446546 7_4:+21328292 None:intergenic
ACTTAACTCAAAACTCAAAT+GGG 0.453388 7_4:-21326168 Msa1144820:exon
AGCTTTGGTGCCTAGCTACT+AGG 0.463643 7_4:-21326098 Msa1144820:three_prime_UTR
CTCTCAGATGAACACTTCAA+TGG 0.470119 7_4:-21328190 Msa1144820:CDS
TGGTAACAAAGGCATGTCAT+AGG 0.475683 7_4:+21328015 None:intergenic
AATGAACAAGATCAGAATAA+GGG 0.476316 7_4:+21328345 None:intergenic
AATAACAAAGAAGTAAAGAT+TGG 0.476661 7_4:+21326222 None:intergenic
GATTGTGGGTCAGATCCATC+AGG 0.479917 7_4:+21326330 None:intergenic
TCACATGTTTCTGAATCATC+TGG 0.486932 7_4:+21327995 None:intergenic
ACTTCAAATTCAAGCAAACT+AGG 0.488204 7_4:-21326477 Msa1144820:CDS
TATAACCACGCAGCTAGAGC+AGG 0.490667 7_4:+21328091 None:intergenic
AATGGATTTCCTGGTCTTGG+AGG 0.496142 7_4:-21327764 Msa1144820:CDS
ACCACATCACAAGTCTCATT+AGG 0.501623 7_4:+21327923 None:intergenic
TCAGCTTGTTCAGGAAAGGC+AGG 0.508237 7_4:+21328235 None:intergenic
GATGTGGTTTATTGCTATTG+TGG 0.516968 7_4:-21327908 Msa1144820:CDS
TATTAAGAGTGCATGATTGT+GGG 0.526493 7_4:+21326316 None:intergenic
ACATAAAACTAACCGAATAG+AGG 0.527430 7_4:+21327720 None:intergenic
GTTCATCTGAGAGACTTAAA+GGG 0.530502 7_4:+21328200 None:intergenic
GCTTTGATGTTGAGCCCTGA+TGG 0.531610 7_4:-21326345 Msa1144820:CDS
TTACATTCACACGGTTACTT+GGG 0.542923 7_4:-21326376 Msa1144820:CDS
CAGGGCAACATCTGCTACGG+TGG 0.543905 7_4:+21328110 None:intergenic
TCATGCACTCTTAATAGTGA+TGG 0.547682 7_4:-21326309 Msa1144820:CDS
CTATTAAGAGTGCATGATTG+TGG 0.554864 7_4:+21326315 None:intergenic
AACATCAGCTTGTTCAGGAA+AGG 0.557995 7_4:+21328231 None:intergenic
GAAAGGCTTTGAAAGTTAGA+GGG 0.558239 7_4:-21327961 Msa1144820:CDS
AAGATCCATAACAAAGATTG+TGG 0.561040 7_4:-21326441 Msa1144820:CDS
AAATTACACTCCTAGTAGCT+AGG 0.563739 7_4:+21326088 None:intergenic
TCAGAAGAATCAACAGCAAG+AGG 0.565150 7_4:+21326282 None:intergenic
GCTCTTGGTGGATTTGAACA+TGG 0.566075 7_4:-21328049 Msa1144820:CDS
GATAATAGAATGAAATGCAT+AGG 0.568060 7_4:+21326192 None:intergenic
GGATGAAAGTGTGAAGCGAT+TGG 0.569007 7_4:-21327819 Msa1144820:CDS
GAAGATGGATTTGGTGTGAG+TGG 0.572672 7_4:+21328271 None:intergenic
ATGTGAGAGTGATTTGGGAA+AGG 0.572811 7_4:-21327978 Msa1144820:CDS
GAGTGAAGTGAATGTGAATG+TGG 0.582346 7_4:+21328421 None:intergenic
GCTGCATCAGGTATAGGGAT+AGG 0.583191 7_4:+21328304 None:intergenic
ATAGGTAGAGACAGATAGCA+AGG 0.586691 7_4:+21328322 None:intergenic
TCTGAATCATCTGGTAACAA+AGG 0.590518 7_4:+21328004 None:intergenic
GAGCAGGGCAACATCTGCTA+CGG 0.598551 7_4:+21328107 None:intergenic
TTAGAGCAACCTCCAAGACC+AGG 0.599435 7_4:+21327755 None:intergenic
CAGCTTGTTCAGGAAAGGCA+GGG 0.600385 7_4:+21328236 None:intergenic
AATGATTCAGAACAGCTCAA+AGG 0.601998 7_4:+21327872 None:intergenic
AGAATAAGGGCATTGAAACA+TGG 0.611713 7_4:+21328358 None:intergenic
TTCAAATCCACCAAGAGCAG+TGG 0.614427 7_4:+21328054 None:intergenic
AAGAAGAAGAGATATGGGTG+TGG 0.621729 7_4:+21328460 None:intergenic
TCATAATGGAAATCTTGTTG+GGG 0.624389 7_4:-21327840 Msa1144820:CDS
ACCTAATGAGACTTGTGATG+TGG 0.641764 7_4:-21327924 Msa1144820:CDS
ATAACCACGCAGCTAGAGCA+GGG 0.647079 7_4:+21328092 None:intergenic
GTGGACTAATGGGATTAACA+TGG 0.649737 7_4:-21326422 Msa1144820:CDS
ATTGTGGGTCAGATCCATCA+GGG 0.665448 7_4:+21326331 None:intergenic
TAGGACAGCTTACATTCACA+CGG 0.684791 7_4:-21326385 Msa1144820:CDS
ACACCAAATCCATCTTCACA+AGG 0.698474 7_4:-21328265 Msa1144820:CDS
GTTGCCCTGCTCTAGCTGCG+TGG 0.703286 7_4:-21328096 Msa1144820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAGAGAAAAAAATTTA+GGG + chr7_4:21327059-21327078 None:intergenic 10.0%
!!! TTTTAGTTATTATTTGAAAT+TGG - chr7_4:21328370-21328389 Msa1144820:CDS 10.0%
!!! TTTAGTTATTATTTGAAATT+GGG - chr7_4:21328371-21328390 Msa1144820:CDS 10.0%
!! TCATTTAAACTTAATCATAA+TGG - chr7_4:21326581-21326600 Msa1144820:intron 15.0%
!! AAATAGAGAAAAAAATTTAG+GGG + chr7_4:21327058-21327077 None:intergenic 15.0%
!! CAAAATAGAGAAAAAAATTT+AGG + chr7_4:21327060-21327079 None:intergenic 15.0%
!!! AGACATCATAATAAATTTTA+AGG - chr7_4:21327195-21327214 Msa1144820:intron 15.0%
!! AATAAACAAGTACATAAGAA+TGG + chr7_4:21326763-21326782 None:intergenic 20.0%
!!! CTTGTTTATTTGAAATTTGA+TGG - chr7_4:21326773-21326792 Msa1144820:intron 20.0%
!! TATTTCACTAATACAACTAA+TGG + chr7_4:21326917-21326936 None:intergenic 20.0%
!!! CATCATAATAAATTTTAAGG+TGG - chr7_4:21327198-21327217 Msa1144820:intron 20.0%
!!! TTTAAAGCCAAATAATTAGT+AGG - chr7_4:21327742-21327761 Msa1144820:CDS 20.0%
!! ATTTCAACCTACTAATTATT+TGG + chr7_4:21327752-21327771 None:intergenic 20.0%
!!! TTTGACTATTTTTCAATGAA+TGG - chr7_4:21327823-21327842 Msa1144820:CDS 20.0%
!! TTTGGCATAATAAATATGTT+TGG + chr7_4:21327857-21327876 None:intergenic 20.0%
!! AATAACAAAGAAGTAAAGAT+TGG + chr7_4:21328216-21328235 None:intergenic 20.0%
!!! GTATATTTGAATATTAGCTT+TGG - chr7_4:21328322-21328341 Msa1144820:CDS 20.0%
! GAAAAAAGAAGAAGAGATAT+GGG + chr7_4:21325983-21326002 None:intergenic 25.0%
! TGAAAAAAGAAGAAGAGATA+TGG + chr7_4:21325984-21326003 None:intergenic 25.0%
! AATGAACAAGATCAGAATAA+GGG + chr7_4:21326093-21326112 None:intergenic 25.0%
!! TTTATTGCTATTGTGGTATT+AGG - chr7_4:21326534-21326553 Msa1144820:intron 25.0%
!! AATCATAATGGAAATCTTGT+TGG - chr7_4:21326593-21326612 Msa1144820:intron 25.0%
!! ATCATAATGGAAATCTTGTT+GGG - chr7_4:21326594-21326613 Msa1144820:intron 25.0%
! AATTAAACTTACATCATGCT+TGG - chr7_4:21326821-21326840 Msa1144820:intron 25.0%
!! CTTTTAACCTTTCTTTACTT+TGG - chr7_4:21326860-21326879 Msa1144820:intron 25.0%
!! TGAAGACATTCATTTTACTA+TGG + chr7_4:21326942-21326961 None:intergenic 25.0%
! AAATGAATGTCTTCAAATCA+AGG - chr7_4:21326947-21326966 Msa1144820:intron 25.0%
! ATTTGCATCACAAAATTAGA+TGG - chr7_4:21327097-21327116 Msa1144820:intron 25.0%
! GATTTAATAGCAGAACATTA+TGG + chr7_4:21327681-21327700 None:intergenic 25.0%
! TGTTGTCTTAGAAAATAGAT+GGG + chr7_4:21327719-21327738 None:intergenic 25.0%
! ATGTTGTCTTAGAAAATAGA+TGG + chr7_4:21327720-21327739 None:intergenic 25.0%
!! TACAATAAAAGAAGGCATAT+AGG + chr7_4:21327783-21327802 None:intergenic 25.0%
! AGAACACTTACAATAAAAGA+AGG + chr7_4:21327791-21327810 None:intergenic 25.0%
! TTTATTGTAAGTGTTCTTGA+TGG - chr7_4:21327794-21327813 Msa1144820:CDS 25.0%
! GATAATAGAATGAAATGCAT+AGG + chr7_4:21328246-21328265 None:intergenic 25.0%
! ACTTAACTCAAAACTCAAAT+GGG - chr7_4:21328267-21328286 Msa1144820:CDS 25.0%
! AAATATACAAATGCCAAGAT+AGG + chr7_4:21328310-21328329 None:intergenic 25.0%
GAATGAACAAGATCAGAATA+AGG + chr7_4:21326094-21326113 None:intergenic 30.0%
! TCATAATGGAAATCTTGTTG+GGG - chr7_4:21326595-21326614 Msa1144820:intron 30.0%
ACATAAAACTAACCGAATAG+AGG + chr7_4:21326718-21326737 None:intergenic 30.0%
! TTACATCATGCTTGGTAATT+GGG - chr7_4:21326829-21326848 Msa1144820:intron 30.0%
TTAACCTTTCTTTACTTTGG+AGG - chr7_4:21326863-21326882 Msa1144820:intron 30.0%
TAACCTTTCTTTACTTTGGA+GGG - chr7_4:21326864-21326883 Msa1144820:intron 30.0%
ACTACAAGTACAAACACAAA+TGG + chr7_4:21327029-21327048 None:intergenic 30.0%
TAGAGAAAAAAATTTAGGGG+AGG + chr7_4:21327055-21327074 None:intergenic 30.0%
! CATAACTTTTGTCGAAATCA+AGG + chr7_4:21327275-21327294 None:intergenic 30.0%
AGTAGGAAATGTGAATTAGA+GGG + chr7_4:21327414-21327433 None:intergenic 30.0%
AAGTAGGAAATGTGAATTAG+AGG + chr7_4:21327415-21327434 None:intergenic 30.0%
TTCCTACTTAACTCAGTTAT+TGG - chr7_4:21327426-21327445 Msa1144820:intron 30.0%
TTCCAATAACTGAGTTAAGT+AGG + chr7_4:21327431-21327450 None:intergenic 30.0%
AAGAACGATAAATCCAAATG+TGG + chr7_4:21327474-21327493 None:intergenic 30.0%
TTAGAAAATAGATGGGAATC+GGG + chr7_4:21327712-21327731 None:intergenic 30.0%
CTTAGAAAATAGATGGGAAT+CGG + chr7_4:21327713-21327732 None:intergenic 30.0%
ACTTCAAATTCAAGCAAACT+AGG - chr7_4:21327958-21327977 Msa1144820:CDS 30.0%
! ACAATCTTTGTTATGGATCT+TGG + chr7_4:21327995-21328014 None:intergenic 30.0%
AAGATCCATAACAAAGATTG+TGG - chr7_4:21327994-21328013 Msa1144820:CDS 30.0%
TTAGTCCACAATCTTTGTTA+TGG + chr7_4:21328002-21328021 None:intergenic 30.0%
! TAACAAAGATTGTGGACTAA+TGG - chr7_4:21328002-21328021 Msa1144820:CDS 30.0%
! AACAAAGATTGTGGACTAAT+GGG - chr7_4:21328003-21328022 Msa1144820:CDS 30.0%
! TATTAAGAGTGCATGATTGT+GGG + chr7_4:21328122-21328141 None:intergenic 30.0%
!!! CATTTGAGTTTTGAGTTAAG+TGG + chr7_4:21328268-21328287 None:intergenic 30.0%
CACTTAACTCAAAACTCAAA+TGG - chr7_4:21328266-21328285 Msa1144820:CDS 30.0%
AAAAGATTGATCTCCTATCT+TGG - chr7_4:21328294-21328313 Msa1144820:CDS 30.0%
!! AGAATAAGGGCATTGAAACA+TGG + chr7_4:21326080-21326099 None:intergenic 35.0%
GTTCATCTGAGAGACTTAAA+GGG + chr7_4:21326238-21326257 None:intergenic 35.0%
TGTTCATCTGAGAGACTTAA+AGG + chr7_4:21326239-21326258 None:intergenic 35.0%
TCTGAATCATCTGGTAACAA+AGG + chr7_4:21326434-21326453 None:intergenic 35.0%
! TCACATGTTTCTGAATCATC+TGG + chr7_4:21326443-21326462 None:intergenic 35.0%
AGAAACATGTGAGAGTGATT+TGG - chr7_4:21326451-21326470 Msa1144820:CDS 35.0%
GAAACATGTGAGAGTGATTT+GGG - chr7_4:21326452-21326471 Msa1144820:CDS 35.0%
!! GAAAGGCTTTGAAAGTTAGA+GGG - chr7_4:21326474-21326493 Msa1144820:CDS 35.0%
! GATGTGGTTTATTGCTATTG+TGG - chr7_4:21326527-21326546 Msa1144820:intron 35.0%
AATGATTCAGAACAGCTCAA+AGG + chr7_4:21326566-21326585 None:intergenic 35.0%
! AATCATGTCAATGGATTTCC+TGG - chr7_4:21326662-21326681 Msa1144820:intron 35.0%
! CTTACATCATGCTTGGTAAT+TGG - chr7_4:21326828-21326847 Msa1144820:intron 35.0%
! TACATCATGCTTGGTAATTG+GGG - chr7_4:21326830-21326849 Msa1144820:intron 35.0%
TATCCCTCCAAAGTAAAGAA+AGG + chr7_4:21326870-21326889 None:intergenic 35.0%
!! TTGTTTTGCACATGGGAATT+GGG + chr7_4:21327135-21327154 None:intergenic 35.0%
! AAGGTGGCTCTGTATATATT+TGG - chr7_4:21327214-21327233 Msa1144820:intron 35.0%
CCGTAATTTCGACAAAATCA+CGG + chr7_4:21327245-21327264 None:intergenic 35.0%
! CCGTGATTTTGTCGAAATTA+CGG - chr7_4:21327242-21327261 Msa1144820:intron 35.0%
! AACTTTTGTCGAAATCAAGG+TGG + chr7_4:21327272-21327291 None:intergenic 35.0%
ATTAGTGATTGTGGCATGTT+TGG + chr7_4:21327342-21327361 None:intergenic 35.0%
CAAACCATCATTAGTGATTG+TGG + chr7_4:21327351-21327370 None:intergenic 35.0%
CACAATCACTAATGATGGTT+TGG - chr7_4:21327349-21327368 Msa1144820:intron 35.0%
GTAGGAAATGTGAATTAGAG+GGG + chr7_4:21327413-21327432 None:intergenic 35.0%
! ACAGAAACAGCACTTACTTT+TGG + chr7_4:21327875-21327894 None:intergenic 35.0%
TTACATTCACACGGTTACTT+GGG - chr7_4:21328059-21328078 Msa1144820:CDS 35.0%
! CTATTAAGAGTGCATGATTG+TGG + chr7_4:21328123-21328142 None:intergenic 35.0%
TCATGCACTCTTAATAGTGA+TGG - chr7_4:21328126-21328145 Msa1144820:CDS 35.0%
! AAGTAAAGATTGGTGCTTTG+AGG + chr7_4:21328206-21328225 None:intergenic 35.0%
AAATTACACTCCTAGTAGCT+AGG + chr7_4:21328350-21328369 None:intergenic 35.0%
! TATTAGTCTATCGCAGAATC+TGG - chr7_4:21328449-21328468 Msa1144820:five_prime_UTR 35.0%
AAGAAGAAGAGATATGGGTG+TGG + chr7_4:21325978-21325997 None:intergenic 40.0%
GAGTGAAGTGAATGTGAATG+TGG + chr7_4:21326017-21326036 None:intergenic 40.0%
ATAGGTAGAGACAGATAGCA+AGG + chr7_4:21326116-21326135 None:intergenic 40.0%
ACACCAAATCCATCTTCACA+AGG - chr7_4:21326170-21326189 Msa1144820:exon 40.0%
! GTTCCTTGTGAAGATGGATT+TGG + chr7_4:21326176-21326195 None:intergenic 40.0%
! AACATCAGCTTGTTCAGGAA+AGG + chr7_4:21326207-21326226 None:intergenic 40.0%
CTCTCAGATGAACACTTCAA+TGG - chr7_4:21326245-21326264 Msa1144820:CDS 40.0%
! TGGTAACAAAGGCATGTCAT+AGG + chr7_4:21326423-21326442 None:intergenic 40.0%
! ATGTGAGAGTGATTTGGGAA+AGG - chr7_4:21326457-21326476 Msa1144820:CDS 40.0%
!! GGAAAGGCTTTGAAAGTTAG+AGG - chr7_4:21326473-21326492 Msa1144820:CDS 40.0%
ACCTAATGAGACTTGTGATG+TGG - chr7_4:21326511-21326530 Msa1144820:intron 40.0%
ACCACATCACAAGTCTCATT+AGG + chr7_4:21326515-21326534 None:intergenic 40.0%
AGTAGCAGCAATCATGTCAA+TGG - chr7_4:21326653-21326672 Msa1144820:intron 40.0%
! ACATCATGCTTGGTAATTGG+GGG - chr7_4:21326831-21326850 Msa1144820:intron 40.0%
!!! CTTGTTTTGCACATGGGAAT+TGG + chr7_4:21327136-21327155 None:intergenic 40.0%
!!! CAACAGCTTGTTTTGCACAT+GGG + chr7_4:21327142-21327161 None:intergenic 40.0%
CATGCCACAATCACTAATGA+TGG - chr7_4:21327344-21327363 Msa1144820:intron 40.0%
ACAACATCTGTAGCCACATT+TGG - chr7_4:21327458-21327477 Msa1144820:intron 40.0%
! GTGGACTAATGGGATTAACA+TGG - chr7_4:21328013-21328032 Msa1144820:CDS 40.0%
CATGGCTTCTTGCTAAGAAT+AGG - chr7_4:21328031-21328050 Msa1144820:CDS 40.0%
TAGGACAGCTTACATTCACA+CGG - chr7_4:21328050-21328069 Msa1144820:CDS 40.0%
CTTACATTCACACGGTTACT+TGG - chr7_4:21328058-21328077 Msa1144820:CDS 40.0%
TCAGAAGAATCAACAGCAAG+AGG + chr7_4:21328156-21328175 None:intergenic 40.0%
CTGTTGCTGCATCAGGTATA+GGG + chr7_4:21326139-21326158 None:intergenic 45.0%
ACTGTTGCTGCATCAGGTAT+AGG + chr7_4:21326140-21326159 None:intergenic 45.0%
!! GAAGATGGATTTGGTGTGAG+TGG + chr7_4:21326167-21326186 None:intergenic 45.0%
GGGATTGTTCCTTGTGAAGA+TGG + chr7_4:21326182-21326201 None:intergenic 45.0%
! CTTGCAACATCAGCTTGTTC+AGG + chr7_4:21326212-21326231 None:intergenic 45.0%
! AGCAGCATGCTTGTTGGATT+TGG + chr7_4:21326291-21326310 None:intergenic 45.0%
ATCATCAGCAGCATGCTTGT+TGG + chr7_4:21326297-21326316 None:intergenic 45.0%
TTCAAATCCACCAAGAGCAG+TGG + chr7_4:21326384-21326403 None:intergenic 45.0%
!! GCTCTTGGTGGATTTGAACA+TGG - chr7_4:21326386-21326405 Msa1144820:CDS 45.0%
! GGATGAAAGTGTGAAGCGAT+TGG - chr7_4:21326616-21326635 Msa1144820:intron 45.0%
! GTCAATGGATTTCCTGGTCT+TGG - chr7_4:21326668-21326687 Msa1144820:intron 45.0%
!! AATGGATTTCCTGGTCTTGG+AGG - chr7_4:21326671-21326690 Msa1144820:intron 45.0%
GTGCTTGCATAGCCTCTATT+CGG - chr7_4:21326703-21326722 Msa1144820:intron 45.0%
!!! GCAACAGCTTGTTTTGCACA+TGG + chr7_4:21327143-21327162 None:intergenic 45.0%
GAACCACGGTGAATTTGACA+CGG + chr7_4:21327320-21327339 None:intergenic 45.0%
! TTGTGGCATGTTTGGAACCA+CGG + chr7_4:21327334-21327353 None:intergenic 45.0%
ATTGTGGGTCAGATCCATCA+GGG + chr7_4:21328107-21328126 None:intergenic 45.0%
!! AAAATTATTATTAAAAATGT+TGG - chr7_4:21327570-21327589 Msa1144820:intron 5.0%
GCTGCATCAGGTATAGGGAT+AGG + chr7_4:21326134-21326153 None:intergenic 50.0%
GGTTGAACTGTTGCTGCATC+AGG + chr7_4:21326146-21326165 None:intergenic 50.0%
! CAGCTTGTTCAGGAAAGGCA+GGG + chr7_4:21326202-21326221 None:intergenic 50.0%
! TCAGCTTGTTCAGGAAAGGC+AGG + chr7_4:21326203-21326222 None:intergenic 50.0%
ATAACCACGCAGCTAGAGCA+GGG + chr7_4:21326346-21326365 None:intergenic 50.0%
TATAACCACGCAGCTAGAGC+AGG + chr7_4:21326347-21326366 None:intergenic 50.0%
TTAGAGCAACCTCCAAGACC+AGG + chr7_4:21326683-21326702 None:intergenic 50.0%
TCACCGTGTCAAATTCACCG+TGG - chr7_4:21327314-21327333 Msa1144820:intron 50.0%
GCTTTGATGTTGAGCCCTGA+TGG - chr7_4:21328090-21328109 Msa1144820:CDS 50.0%
GATTGTGGGTCAGATCCATC+AGG + chr7_4:21328108-21328127 None:intergenic 50.0%
!! AGCTTTGGTGCCTAGCTACT+AGG - chr7_4:21328337-21328356 Msa1144820:CDS 50.0%
GAGCAGGGCAACATCTGCTA+CGG + chr7_4:21326331-21326350 None:intergenic 55.0%
GCTTACTCTGCCACTGCTCT+TGG - chr7_4:21326371-21326390 Msa1144820:CDS 55.0%
TACTCTGCCACTGCTCTTGG+TGG - chr7_4:21326374-21326393 Msa1144820:CDS 55.0%
CAGGGCAACATCTGCTACGG+TGG + chr7_4:21326328-21326347 None:intergenic 60.0%
GTTGCCCTGCTCTAGCTGCG+TGG - chr7_4:21326339-21326358 Msa1144820:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 21325966 21328491 21325966 ID=Msa1144820;Name=Msa1144820
chr7_4 mRNA 21325966 21328491 21325966 ID=Msa1144820-mRNA-1;Parent=Msa1144820;Name=Msa1144820-mRNA-1;_AED=0.03;_eAED=0.03;_QI=96|1|1|1|1|1|2|219|329
chr7_4 exon 21325966 21326511 21325966 ID=Msa1144820-mRNA-1:exon:5125;Parent=Msa1144820-mRNA-1
chr7_4 exon 21327733 21328491 21327733 ID=Msa1144820-mRNA-1:exon:5124;Parent=Msa1144820-mRNA-1
chr7_4 five_prime_UTR 21328396 21328491 21328396 ID=Msa1144820-mRNA-1:five_prime_utr;Parent=Msa1144820-mRNA-1
chr7_4 CDS 21327733 21328395 21327733 ID=Msa1144820-mRNA-1:cds;Parent=Msa1144820-mRNA-1
chr7_4 CDS 21326185 21326511 21326185 ID=Msa1144820-mRNA-1:cds;Parent=Msa1144820-mRNA-1
chr7_4 three_prime_UTR 21325966 21326184 21325966 ID=Msa1144820-mRNA-1:three_prime_utr;Parent=Msa1144820-mRNA-1
Gene Sequence

>Msa1144820

ATGACACTGACACTACCATGTTTCAATGCCCTTATTCTGATCTTGTTCATTCCTTGCTATCTGTCTCTACCTATCCCTATACCTGATGCAGCAACAGTTCAACCACTCACACCAAATCCATCTTCACAAGGAACAATCCCTGCCTTTCCTGAACAAGCTGATGTTGCAAGATGCCCTTTAAGTCTCTCAGATGAACACTTCAATGGAATCAAAAACGCATGCAGTTCCAAATCCAACAAGCATGCTGCTGATGATGATGAACTCCACCGTAGCAGATGTTGCCCTGCTCTAGCTGCGTGGTTATATTCTGCTTACTCTGCCACTGCTCTTGGTGGATTTGAACATGGACACACTACATCCTATGACATGCCTTTGTTACCAGATGATTCAGAAACATGTGAGAGTGATTTGGGAAAGGCTTTGAAAGTTAGAGGGATTGAACTTTTTCAACCTAATGAGACTTGTGATGTGGTTTATTGCTATTGTGGTATTAGGCTTCATCCTTTGAGCTGTTCTGAATCATTTAAACTTAATCATAATGGAAATCTTGTTGGGGATGAAAGTGTGAAGCGATTGGAGAGAAATTGTTTGAGTAGCAGCAATCATGTCAATGGATTTCCTGGTCTTGGAGGTTGCTCTAAGTGCTTGCATAGCCTCTATTCGCTTAGAAAGAAGACTTCAAATTCAAGCAAACTAGGAGATAGAACAACCAAGATCCATAACAAAGATTGTGGACTAATGGGATTAACATGGCTTCTTGCTAAGAATAGGACAGCTTACATTCACACGGTTACTTGGGTTCTTCGTGCTTTGATGTTGAGCCCTGATGGATCTGACCCACAATCATGCACTCTTAATAGTGATGGAATGCCTCTTGCTGTTGATTCTTCTGAAATGTATGATCAATCTTCATCAATCAACCTCAAAGCACCAATCTTTACTTCTTTGTTATTTCTACTACCTATGCATTTCATTCTATTATCCACTTAA
Protein sequence

>Msa1144820

MTLTLPCFNALILILFIPCYLSLPIPIPDAATVQPLTPNPSSQGTIPAFPEQADVARCPLSLSDEHFNGIKNACSSKSNKHAADDDELHRSRCCPALAAWLYSAYSATALGGFEHGHTTSYDMPLLPDDSETCESDLGKALKVRGIELFQPNETCDVVYCYCGIRLHPLSCSESFKLNHNGNLVGDESVKRLERNCLSSSNHVNGFPGLGGCSKCLHSLYSLRKKTSNSSKLGDRTTKIHNKDCGLMGLTWLLAKNRTAYIHTVTWVLRALMLSPDGSDPQSCTLNSDGMPLAVDSSEMYDQSSSINLKAPIFTSLLFLLPMHFILLST*