AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1152910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1152910 MtrunA17_Chr7g0243051 92.857 224 16 0 1 224 1 224 1.56e-154 427
Msa1152910 MtrunA17_Chr4g0007601 58.559 222 72 3 1 220 1 204 1.58e-77 233
Msa1152910 MtrunA17_Chr6g0473621 45.000 220 118 2 3 220 18 236 1.26e-54 178
Msa1152910 MtrunA17_Chr1g0203771 42.986 221 125 1 1 220 4 224 7.51e-54 176
Msa1152910 MtrunA17_Chr2g0285591 37.864 206 126 2 17 222 28 231 2.82e-44 148
Msa1152910 MtrunA17_Chr4g0057261 43.137 204 113 2 13 216 28 228 4.73e-44 149
Msa1152910 MtrunA17_Chr6g0458281 44.068 118 58 2 10 123 23 136 4.55e-24 93.6
Msa1152910 MtrunA17_Chr6g0458271 46.078 102 52 1 16 117 35 133 6.62e-24 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1152910 AT3G51780.1 47.032 219 94 4 19 220 10 223 2.41e-58 185
Msa1152910 AT5G52060.1 43.069 202 113 1 21 220 41 242 4.69e-47 159
Msa1152910 AT5G07220.1 40.271 221 127 3 1 220 4 220 9.27e-45 151
Msa1152910 AT5G62100.2 37.019 208 129 1 16 223 12 217 1.16e-39 138
Msa1152910 AT5G62100.1 35.616 219 128 3 16 223 12 228 1.54e-36 130
Msa1152910 AT5G62100.5 35.385 195 120 3 10 202 6 196 1.46e-34 122
Msa1152910 AT5G62100.4 34.872 195 121 3 10 202 6 196 6.29e-34 121
Msa1152910 AT5G62100.3 35.616 146 88 3 10 153 6 147 1.11e-23 92.8
Msa1152910 AT5G62100.6 35.616 146 88 3 10 153 6 147 1.11e-23 92.8
Msa1152910 AT5G14360.1 47.573 103 46 2 17 117 46 142 2.54e-23 92.4
Msa1152910 AT5G40630.1 41.905 105 57 3 14 117 51 152 9.46e-20 83.2

Find 77 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCAAATACTCTTCTTTAA+AGG 0.201663 7_4:+38955267 Msa1152910:CDS
GCATTACCAATGTTTCTATA+AGG 0.228645 7_4:-38957176 None:intergenic
TGCCTTTGAAGAATACAAAA+AGG 0.290976 7_4:+38957450 Msa1152910:three_prime_UTR
CAATTGGAGACCTTTGACTT+TGG 0.294069 7_4:+38957215 Msa1152910:three_prime_UTR
AAGAAGAGTATTTGCTGTTC+AGG 0.301501 7_4:-38955261 None:intergenic
GAAATCAAAACGCGAGTAAT+TGG 0.308015 7_4:-38953810 None:intergenic
GCTGGAGCTGGATGGTATTA+AGG 0.331947 7_4:+38955701 Msa1152910:CDS
GAACCTTGATTCTTCATTTG+AGG 0.350916 7_4:+38957379 Msa1152910:three_prime_UTR
AAACTGCTTGCACACAAAAC+TGG 0.371537 7_4:+38955231 Msa1152910:CDS
AACACCGCCGCTTATTCTTC+CGG 0.374973 7_4:+38953849 Msa1152910:CDS
TACTACACGTTACTCAAGAT+TGG 0.391701 7_4:+38957289 Msa1152910:three_prime_UTR
TTCGTACAGACACGCGAATC+AGG 0.397002 7_4:+38953927 Msa1152910:CDS
CATTACCAATGTTTCTATAA+GGG 0.407817 7_4:-38957175 None:intergenic
GCCTTGGATGTGGCTGTCAA+TGG 0.409273 7_4:+38955618 Msa1152910:CDS
GTTGCTTATGAGGAAATTGC+TGG 0.415619 7_4:+38955683 Msa1152910:CDS
TTACTACAGAGTTGCTTATG+AGG 0.419353 7_4:+38955673 Msa1152910:CDS
CTTGAGTAACGTGTAGTAGA+TGG 0.435924 7_4:-38957284 None:intergenic
AAAGTCAAAGGTCTCCAATT+GGG 0.443694 7_4:-38957213 None:intergenic
GGCAATATCTTTAAACACTT+GGG 0.450067 7_4:+38957426 Msa1152910:three_prime_UTR
CAAAGTCAAAGGTCTCCAAT+TGG 0.452077 7_4:-38957214 None:intergenic
AGGCAATATCTTTAAACACT+TGG 0.452622 7_4:+38957425 Msa1152910:three_prime_UTR
AAATTCTTTGTCAGAAACCT+TGG 0.457775 7_4:-38955646 None:intergenic
CGGTGTCTCGCCGCCGAATG+AGG 0.463239 7_4:+38953869 Msa1152910:CDS
GCAGGTGTTGGCCTTGGATG+TGG 0.469671 7_4:+38955608 Msa1152910:intron
CATTCGATATCCGCCTCATT+CGG 0.471255 7_4:-38953882 None:intergenic
CTTCTTTAAAGGAAAAGAAA+AGG 0.482417 7_4:+38955278 Msa1152910:CDS
GAAGAGAGTCTCACAGAAAG+AGG 0.485676 7_4:-38954036 None:intergenic
CTCATTCGGCGGCGAGACAC+CGG 0.488256 7_4:-38953868 None:intergenic
GAATAAGCGGCGGTGTTCAT+CGG 0.491708 7_4:-38953843 None:intergenic
TATGAGGAAATTGCTGGAGC+TGG 0.493825 7_4:+38955689 Msa1152910:CDS
TTGGATGTGGCTGTCAATGG+AGG 0.496098 7_4:+38955621 Msa1152910:CDS
ACTACACGTTACTCAAGATT+GGG 0.498336 7_4:+38957290 Msa1152910:three_prime_UTR
TTTCAAGCTGTTGCTGTAGT+CGG 0.509391 7_4:+38955426 Msa1152910:CDS
AGTCTCAGCAACAACCCAAT+TGG 0.512112 7_4:+38957199 Msa1152910:three_prime_UTR
AGGTCGACAAGCTTTGTGAC+AGG 0.514120 7_4:+38955454 Msa1152910:CDS
GGTCCTCAAATGAAGAATCA+AGG 0.541023 7_4:-38957382 None:intergenic
TCACAGAAAGAGGATTGAGC+TGG 0.549543 7_4:-38954026 None:intergenic
CGAATGAGGCGGATATCGAA+TGG 0.551079 7_4:+38953883 Msa1152910:CDS
GGAGACCTTTGACTTTGGAA+TGG 0.552665 7_4:+38957220 Msa1152910:three_prime_UTR
GAAGAGAATTTGCACATGGA+AGG 0.561882 7_4:+38955306 Msa1152910:CDS
GATGTTGATCATCGGTCCAT+CGG 0.564771 7_4:-38953964 None:intergenic
ATCAACATCAGTGTTGCGTA+TGG 0.567834 7_4:+38953978 Msa1152910:CDS
GAATGAGGCGGATATCGAAT+GGG 0.572222 7_4:+38953884 Msa1152910:CDS
GTCTGTACGAACATACCGCC+GGG 0.573976 7_4:-38953915 None:intergenic
GCTTACCATTCCAAAGTCAA+AGG 0.581977 7_4:-38957225 None:intergenic
GAATTTGCACATGGAAGGTG+TGG 0.586370 7_4:+38955311 Msa1152910:CDS
GCCTACCCTTATAGAAACAT+TGG 0.588082 7_4:+38957170 Msa1152910:exon
AAGTATGAGATGACGAGAGG+AGG 0.589308 7_4:+38953733 Msa1152910:five_prime_UTR
ATGTTTCTATAAGGGTAGGC+GGG 0.590318 7_4:-38957167 None:intergenic
GGTCGACAAGCTTTGTGACA+GGG 0.595734 7_4:+38955455 Msa1152910:CDS
GGTGATAATGACGATGAGGT+TGG 0.599854 7_4:-38957257 None:intergenic
TGGATGTGGCTGTCAATGGA+GGG 0.601202 7_4:+38955622 Msa1152910:CDS
ACCAATGTTTCTATAAGGGT+AGG 0.604431 7_4:-38957171 None:intergenic
CTGGATGGTATTAAGGCTGA+AGG 0.605506 7_4:+38955708 Msa1152910:CDS
CGAGACACCGGAAGAATAAG+CGG 0.607254 7_4:-38953856 None:intergenic
TGCAAGATCAATACTCACAA+TGG 0.610590 7_4:-38957539 None:intergenic
GGCTGTCAATGGAGGGACCA+AGG 0.612660 7_4:+38955629 Msa1152910:CDS
AGGAAATTGCTGGAGCTGGA+TGG 0.613422 7_4:+38955693 Msa1152910:CDS
TTTGCACATGGAAGGTGTGG+AGG 0.613848 7_4:+38955314 Msa1152910:CDS
TCCATTGACAGCCACATCCA+AGG 0.628433 7_4:-38955619 None:intergenic
TGTTGCTGTAGTCGGATCAG+AGG 0.628867 7_4:+38955434 Msa1152910:CDS
AATGTTTCTATAAGGGTAGG+CGG 0.632352 7_4:-38957168 None:intergenic
ACGGGGAAAGACAAGAGAAG+AGG 0.635564 7_4:-38953774 None:intergenic
TGGCATAGAGAGTAATCTTG+AGG 0.638606 7_4:+38955371 Msa1152910:CDS
TGATACTTTAGATTCCCTGA+AGG 0.643562 7_4:+38957135 Msa1152910:CDS
TAAGTTGCAGAGAAAAGCTG+AGG 0.643704 7_4:+38955737 Msa1152910:CDS
GCAACACTGATGTTGATCAT+CGG 0.648002 7_4:-38953972 None:intergenic
AGAAAGTATGAGATGACGAG+AGG 0.660265 7_4:+38953730 Msa1152910:five_prime_UTR
GAATGAAGAGAATTTGCACA+TGG 0.664413 7_4:+38955302 Msa1152910:CDS
TATTAACGAACTAAAACACA+AGG 0.670026 7_4:+38957639 Msa1152910:three_prime_UTR
GGTGACGATGATCATACCGA+TGG 0.677266 7_4:+38953948 Msa1152910:CDS
TAACGTGTAGTAGATGGTGG+TGG 0.679118 7_4:-38957278 None:intergenic
TGAGTATTGATCTTGCATGA+CGG 0.703961 7_4:+38957545 Msa1152910:three_prime_UTR
TGGTGGTGATAATGACGATG+AGG 0.706758 7_4:-38957261 None:intergenic
GACACCGGAAGAATAAGCGG+CGG 0.727415 7_4:-38953853 None:intergenic
GAGTAACGTGTAGTAGATGG+TGG 0.730515 7_4:-38957281 None:intergenic
GAATGGGAGATGCGTCCCGG+CGG 0.755454 7_4:+38953900 Msa1152910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAATAATAATAATAT+GGG - chr7_4:38955494-38955513 None:intergenic 0.0%
!! TAATAATAATAATAATAATA+TGG - chr7_4:38955495-38955514 None:intergenic 0.0%
!! ATTAAAAATATAAAGTAAGA+AGG + chr7_4:38953681-38953700 Msa1152910:five_prime_UTR 10.0%
!! TTAAAAATATAAAGTAAGAA+GGG + chr7_4:38953682-38953701 Msa1152910:five_prime_UTR 10.0%
!! ATCATTCATATTATATTTAA+AGG + chr7_4:38957052-38957071 Msa1152910:intron 10.0%
!! TAAAAATTTACTTAGTTACT+TGG + chr7_4:38954210-38954229 Msa1152910:intron 15.0%
!! GAATGTATAATATGAAATTA+TGG + chr7_4:38954272-38954291 Msa1152910:intron 15.0%
!!! ATTAGCTTACATAAAATTAA+AGG - chr7_4:38954683-38954702 None:intergenic 15.0%
!!! TATAACATATAATTTGCTTA+AGG - chr7_4:38955019-38955038 None:intergenic 15.0%
!!! AGAAAATATTTTGTTTTCAT+TGG + chr7_4:38955162-38955181 Msa1152910:intron 15.0%
!!! AGTTTTTAAAATGAATCTTT+CGG + chr7_4:38956280-38956299 Msa1152910:intron 15.0%
!!! TAATTAACAAATTTTTCTTG+AGG - chr7_4:38956326-38956345 None:intergenic 15.0%
!!! ATTTGTTTTTAAAATTCCAA+TGG + chr7_4:38956424-38956443 Msa1152910:intron 15.0%
!!! AAATGTTTTTGATAGTTGAA+AGG + chr7_4:38954595-38954614 Msa1152910:intron 20.0%
!!! AATGTTTTTGATAGTTGAAA+GGG + chr7_4:38954596-38954615 Msa1152910:intron 20.0%
!!! CTTTATTTATGTATTAGTGT+AGG + chr7_4:38954897-38954916 Msa1152910:intron 20.0%
!! ATCTTGATATAGAATATGTT+TGG + chr7_4:38955088-38955107 Msa1152910:intron 20.0%
!! TCTTGATATAGAATATGTTT+GGG + chr7_4:38955089-38955108 Msa1152910:intron 20.0%
!!! TTGATTTAGTCTTTAATAAC+AGG + chr7_4:38955198-38955217 Msa1152910:intron 20.0%
!!! TGATTTAGTCTTTAATAACA+GGG + chr7_4:38955199-38955218 Msa1152910:intron 20.0%
!! AATAATAATATGGGAAACAT+GGG - chr7_4:38955485-38955504 None:intergenic 20.0%
!! TAATAATAATATGGGAAACA+TGG - chr7_4:38955486-38955505 None:intergenic 20.0%
!! CATTATAGAAACATAATAAC+TGG + chr7_4:38955550-38955569 Msa1152910:intron 20.0%
!!! ATGAATTTTAAATCATCACT+TGG + chr7_4:38955877-38955896 Msa1152910:intron 20.0%
!!! TATATACTTTTTTCTTGTTG+AGG + chr7_4:38956393-38956412 Msa1152910:intron 20.0%
!!! AAGTTATCTAACCATAATTT+TGG + chr7_4:38956543-38956562 Msa1152910:intron 20.0%
!! AAATATCAACACCAAAATTA+TGG - chr7_4:38956557-38956576 None:intergenic 20.0%
!!! ATAATTTTGGTGTTGATATT+TGG + chr7_4:38956556-38956575 Msa1152910:intron 20.0%
!! GGTGATATTTATAATTTACT+GGG - chr7_4:38956798-38956817 None:intergenic 20.0%
!! AGGTGATATTTATAATTTAC+TGG - chr7_4:38956799-38956818 None:intergenic 20.0%
!!! TTTTTTTTTTACCTTCAAAC+AGG + chr7_4:38957090-38957109 Msa1152910:intron 20.0%
!! TTAAGCAAACTACAAAAAAT+CGG - chr7_4:38957406-38957425 None:intergenic 20.0%
!!! TTTTAACGTATAGGTAAATT+TGG + chr7_4:38957600-38957619 Msa1152910:three_prime_UTR 20.0%
!! TTCTCTCTATGTTGATATTT+TGG + chr7_4:38954056-38954075 Msa1152910:intron 25.0%
! TTTACTTAGTTACTTGGATT+AGG + chr7_4:38954216-38954235 Msa1152910:intron 25.0%
! TATGAAATTATGGATGAGTT+TGG + chr7_4:38954282-38954301 Msa1152910:intron 25.0%
! GTCAATATATCTAACCATTT+AGG - chr7_4:38954556-38954575 None:intergenic 25.0%
!!! ATGTTTTTGATAGTTGAAAG+GGG + chr7_4:38954597-38954616 Msa1152910:intron 25.0%
! AACTTGTAATGACAATACTA+GGG + chr7_4:38954620-38954639 Msa1152910:intron 25.0%
! AAGCTAATACATATTAGTGA+AGG + chr7_4:38954695-38954714 Msa1152910:intron 25.0%
! AAGAAAGAAAGATTGATGAA+TGG - chr7_4:38954735-38954754 None:intergenic 25.0%
! TTCTTCAACACTATTTGTTT+GGG - chr7_4:38954758-38954777 None:intergenic 25.0%
! AATTCTCGATGCAAATTAAA+TGG - chr7_4:38954868-38954887 None:intergenic 25.0%
!! TATTACTAGTTTACTCACTA+AGG - chr7_4:38954946-38954965 None:intergenic 25.0%
!! AGTTGATTAACTTGAAAACT+TGG + chr7_4:38954991-38955010 Msa1152910:intron 25.0%
!! GTTGATTAACTTGAAAACTT+GGG + chr7_4:38954992-38955011 Msa1152910:intron 25.0%
! CTTGATATAGAATATGTTTG+GGG + chr7_4:38955090-38955109 Msa1152910:intron 25.0%
!! GATTTAGTCTTTAATAACAG+GGG + chr7_4:38955200-38955219 Msa1152910:intron 25.0%
!! CTTCTTTAAAGGAAAAGAAA+AGG + chr7_4:38955278-38955297 Msa1152910:CDS 25.0%
!!! GTTACTGATTTTAATTTTGC+AGG + chr7_4:38955590-38955609 Msa1152910:intron 25.0%
!! CAAAAGAGTATATTCATGTT+AGG + chr7_4:38956131-38956150 Msa1152910:intron 25.0%
! TCTAATCAATATTATGAGTC+AGG - chr7_4:38956203-38956222 None:intergenic 25.0%
!!! TGATATTTGGTGATGTTAAT+GGG + chr7_4:38956462-38956481 Msa1152910:intron 25.0%
!!! TTGTTTTAGGAATGAGTATT+CGG + chr7_4:38956485-38956504 Msa1152910:intron 25.0%
! ACAAATAAGCTTGAAGAAAA+AGG - chr7_4:38956596-38956615 None:intergenic 25.0%
! GAACAATTCAAATTTCCTTA+CGG - chr7_4:38956660-38956679 None:intergenic 25.0%
! TAGAAAGCAACAATATAACA+TGG - chr7_4:38956710-38956729 None:intergenic 25.0%
! TTACAGGAAGAAGAAAAAAA+TGG - chr7_4:38956768-38956787 None:intergenic 25.0%
! AAAAAAAAAAACCTCTGTTC+AGG - chr7_4:38957078-38957097 None:intergenic 25.0%
!! CATTACCAATGTTTCTATAA+GGG - chr7_4:38957178-38957197 None:intergenic 25.0%
! AGAATCAAGGTTCAAAAATA+TGG - chr7_4:38957372-38957391 None:intergenic 25.0%
!!! GATTTTTTGTAGTTTGCTTA+AGG + chr7_4:38957405-38957424 Msa1152910:three_prime_UTR 25.0%
!!! TCATACTGTTTTTAACGTAT+AGG + chr7_4:38957591-38957610 Msa1152910:three_prime_UTR 25.0%
! TATTAACGAACTAAAACACA+AGG + chr7_4:38957639-38957658 Msa1152910:three_prime_UTR 25.0%
!! AATTGGTGCGTTTTGATAAA+CGG - chr7_4:38953796-38953815 None:intergenic 30.0%
! GTTGAATTTTCGTTTGATCT+TGG + chr7_4:38954086-38954105 Msa1152910:intron 30.0%
! TTGGATTAGGTTAATGATGA+TGG + chr7_4:38954229-38954248 Msa1152910:intron 30.0%
! TGGATTAGGTTAATGATGAT+GGG + chr7_4:38954230-38954249 Msa1152910:intron 30.0%
TGTGATGATTGTGTTGAAAA+TGG + chr7_4:38954516-38954535 Msa1152910:intron 30.0%
!! ATATTGACTTCTTCTCTTTC+AGG + chr7_4:38954568-38954587 Msa1152910:intron 30.0%
GAACTTGTAATGACAATACT+AGG + chr7_4:38954619-38954638 Msa1152910:intron 30.0%
CTTCTTCAACACTATTTGTT+TGG - chr7_4:38954759-38954778 None:intergenic 30.0%
GAAATTTGAATTTCGAACTG+AGG + chr7_4:38954803-38954822 Msa1152910:intron 30.0%
TATTACTGTGTTACAAAGAG+TGG + chr7_4:38954837-38954856 Msa1152910:intron 30.0%
CAGCAAATACTCTTCTTTAA+AGG + chr7_4:38955267-38955286 Msa1152910:CDS 30.0%
!!! GATTTTAATTTTGCAGGTGT+TGG + chr7_4:38955596-38955615 Msa1152910:intron 30.0%
AAATTCTTTGTCAGAAACCT+TGG - chr7_4:38955649-38955668 None:intergenic 30.0%
AAAAGCTGAGGTAATGTTAA+TGG + chr7_4:38955749-38955768 Msa1152910:intron 30.0%
TGACCTAGCATAAAAAGTAA+TGG - chr7_4:38955813-38955832 None:intergenic 30.0%
!!! TTACTTTTTATGCTAGGTCA+TGG + chr7_4:38955813-38955832 Msa1152910:intron 30.0%
! TTGCTATGATTTTTGTGAAG+TGG + chr7_4:38955905-38955924 Msa1152910:intron 30.0%
TGTTAAAGAGCAGTATATCA+TGG - chr7_4:38955937-38955956 None:intergenic 30.0%
CCAAATACATAGATACTATC+AGG - chr7_4:38956043-38956062 None:intergenic 30.0%
CCTGATAGTATCTATGTATT+TGG + chr7_4:38956040-38956059 Msa1152910:intron 30.0%
TTCAAAGAGATTCATGCAAT+AGG - chr7_4:38956249-38956268 None:intergenic 30.0%
AAATGAATCTTTCGGAACTT+CGG + chr7_4:38956288-38956307 Msa1152910:intron 30.0%
CTATCACTAGAAAACTCTAT+TGG + chr7_4:38956365-38956384 Msa1152910:intron 30.0%
AAAATTCCAATGGTTCTTGA+TGG + chr7_4:38956434-38956453 Msa1152910:intron 30.0%
!! GTGATATTTGGTGATGTTAA+TGG + chr7_4:38956461-38956480 Msa1152910:intron 30.0%
!!! TGATGTTAATGGGTTGTTTT+AGG + chr7_4:38956472-38956491 Msa1152910:intron 30.0%
TTAGATAACTTGACCTCAAA+AGG - chr7_4:38956534-38956553 None:intergenic 30.0%
TGCAATTGTTTATTTCCGTA+AGG + chr7_4:38956642-38956661 Msa1152910:intron 30.0%
AGGAAGAAGAAAAAAATGGT+GGG - chr7_4:38956764-38956783 None:intergenic 30.0%
CAATGAAATCTACTTGTGTT+GGG + chr7_4:38956877-38956896 Msa1152910:intron 30.0%
! TATCCTTTTACACTGAGTTA+CGG - chr7_4:38956975-38956994 None:intergenic 30.0%
ATAAATTCTTGTACTGCTTG+CGG + chr7_4:38956992-38957011 Msa1152910:intron 30.0%
! GCATTACCAATGTTTCTATA+AGG - chr7_4:38957179-38957198 None:intergenic 30.0%
AGGCAATATCTTTAAACACT+TGG + chr7_4:38957425-38957444 Msa1152910:three_prime_UTR 30.0%
GGCAATATCTTTAAACACTT+GGG + chr7_4:38957426-38957445 Msa1152910:three_prime_UTR 30.0%
! TGCCTTTGAAGAATACAAAA+AGG + chr7_4:38957450-38957469 Msa1152910:three_prime_UTR 30.0%
! GCCTTTGAAGAATACAAAAA+GGG + chr7_4:38957451-38957470 Msa1152910:three_prime_UTR 30.0%
! TCCCTTTTTGTATTCTTCAA+AGG - chr7_4:38957455-38957474 None:intergenic 30.0%
GAATACAAAAAGGGAAATGA+AGG + chr7_4:38957460-38957479 Msa1152910:three_prime_UTR 30.0%
!! TTACATGAACCCATTTTTCT+GGG - chr7_4:38957507-38957526 None:intergenic 30.0%
!! ATTACATGAACCCATTTTTC+TGG - chr7_4:38957508-38957527 None:intergenic 30.0%
!! ATTGGTGCGTTTTGATAAAC+GGG - chr7_4:38953795-38953814 None:intergenic 35.0%
GAAATCAAAACGCGAGTAAT+TGG - chr7_4:38953813-38953832 None:intergenic 35.0%
GTACATGTGAGATTGATTGT+TGG + chr7_4:38954337-38954356 Msa1152910:intron 35.0%
GATGATTGTGTTGAAAATGG+TGG + chr7_4:38954519-38954538 Msa1152910:intron 35.0%
TCGATGCAAATTAAATGGAG+GGG - chr7_4:38954863-38954882 None:intergenic 35.0%
CTCGATGCAAATTAAATGGA+GGG - chr7_4:38954864-38954883 None:intergenic 35.0%
TCTCGATGCAAATTAAATGG+AGG - chr7_4:38954865-38954884 None:intergenic 35.0%
!! AAGAAGAGTATTTGCTGTTC+AGG - chr7_4:38955264-38955283 None:intergenic 35.0%
GAATGAAGAGAATTTGCACA+TGG + chr7_4:38955302-38955321 Msa1152910:CDS 35.0%
! CAAGTCAAAGCTTTTGCTTT+TGG + chr7_4:38955338-38955357 Msa1152910:CDS 35.0%
TTACTACAGAGTTGCTTATG+AGG + chr7_4:38955673-38955692 Msa1152910:CDS 35.0%
! GACTTGAAAGCTTTAACTGT+TGG + chr7_4:38955786-38955805 Msa1152910:intron 35.0%
!!! GGTCCATTACTTTTTATGCT+AGG + chr7_4:38955807-38955826 Msa1152910:intron 35.0%
TTATGCTAGGTCATGGAAAA+TGG + chr7_4:38955820-38955839 Msa1152910:intron 35.0%
! AATATTGTTGTTCAAGCCTG+TGG + chr7_4:38955996-38956015 Msa1152910:intron 35.0%
! AAAGTAAAGCTTTGATCCAC+AGG - chr7_4:38956015-38956034 None:intergenic 35.0%
! ATTTTTGCCTAGTACTGCAA+TGG + chr7_4:38956064-38956083 Msa1152910:intron 35.0%
TAAATTGCCATTGCAGTACT+AGG - chr7_4:38956074-38956093 None:intergenic 35.0%
TGCAATGGCAATTTATATGC+TGG + chr7_4:38956079-38956098 Msa1152910:intron 35.0%
GCAATGGCAATTTATATGCT+GGG + chr7_4:38956080-38956099 Msa1152910:intron 35.0%
!!! TTTTTTCTTGTTGAGGTCAC+AGG + chr7_4:38956400-38956419 Msa1152910:intron 35.0%
! CTTGATGGTCAAGTGATATT+TGG + chr7_4:38956449-38956468 Msa1152910:intron 35.0%
CTTGAAGAAAAAGGTAGAGA+AGG - chr7_4:38956587-38956606 None:intergenic 35.0%
CATGGCAACAATTCACAAAT+GGG - chr7_4:38956692-38956711 None:intergenic 35.0%
ACATGGCAACAATTCACAAA+TGG - chr7_4:38956693-38956712 None:intergenic 35.0%
TACATGTGTATGATGTTCTC+TGG + chr7_4:38956728-38956747 Msa1152910:intron 35.0%
CAGGAAGAAGAAAAAAATGG+TGG - chr7_4:38956765-38956784 None:intergenic 35.0%
! TTTACTGGGATCTAGTTTAC+AGG - chr7_4:38956784-38956803 None:intergenic 35.0%
CCAACACAAGTAGATTTCAT+TGG - chr7_4:38956879-38956898 None:intergenic 35.0%
CCAATGAAATCTACTTGTGT+TGG + chr7_4:38956876-38956895 Msa1152910:intron 35.0%
TTGATGTGTTTGTCACTATG+AGG + chr7_4:38956909-38956928 Msa1152910:intron 35.0%
TACACTGAGTTACGGAAAAT+GGG - chr7_4:38956967-38956986 None:intergenic 35.0%
TTACACTGAGTTACGGAAAA+TGG - chr7_4:38956968-38956987 None:intergenic 35.0%
TTTCCGTAACTCAGTGTAAA+AGG + chr7_4:38956969-38956988 Msa1152910:intron 35.0%
ATATTTAAAGGCCTGAACAG+AGG + chr7_4:38957064-38957083 Msa1152910:intron 35.0%
TGATACTTTAGATTCCCTGA+AGG + chr7_4:38957135-38957154 Msa1152910:CDS 35.0%
! AATGTTTCTATAAGGGTAGG+CGG - chr7_4:38957171-38957190 None:intergenic 35.0%
! ACCAATGTTTCTATAAGGGT+AGG - chr7_4:38957174-38957193 None:intergenic 35.0%
AAAGTCAAAGGTCTCCAATT+GGG - chr7_4:38957216-38957235 None:intergenic 35.0%
TACTACACGTTACTCAAGAT+TGG + chr7_4:38957289-38957308 Msa1152910:three_prime_UTR 35.0%
ACTACACGTTACTCAAGATT+GGG + chr7_4:38957290-38957309 Msa1152910:three_prime_UTR 35.0%
GAACCTTGATTCTTCATTTG+AGG + chr7_4:38957379-38957398 Msa1152910:three_prime_UTR 35.0%
ATACTCTTGCCCAGAAAAAT+GGG + chr7_4:38957495-38957514 Msa1152910:three_prime_UTR 35.0%
TGCAAGATCAATACTCACAA+TGG - chr7_4:38957542-38957561 None:intergenic 35.0%
!! TGAGTATTGATCTTGCATGA+CGG + chr7_4:38957545-38957564 Msa1152910:three_prime_UTR 35.0%
! AGAAAGTATGAGATGACGAG+AGG + chr7_4:38953730-38953749 Msa1152910:five_prime_UTR 40.0%
!! TTGGTGCGTTTTGATAAACG+GGG - chr7_4:38953794-38953813 None:intergenic 40.0%
GCAACACTGATGTTGATCAT+CGG - chr7_4:38953975-38953994 None:intergenic 40.0%
ATCAACATCAGTGTTGCGTA+TGG + chr7_4:38953978-38953997 Msa1152910:CDS 40.0%
! TGATTGTTGGAGAGAGCTTT+TGG + chr7_4:38954350-38954369 Msa1152910:intron 40.0%
AACTGAAAAACAGCAGCACA+TGG - chr7_4:38954394-38954413 None:intergenic 40.0%
!! TGGACTTTGCTGTTCCTAAA+TGG + chr7_4:38954539-38954558 Msa1152910:intron 40.0%
AAACTGCTTGCACACAAAAC+TGG + chr7_4:38955231-38955250 Msa1152910:CDS 40.0%
GAAGAGAATTTGCACATGGA+AGG + chr7_4:38955306-38955325 Msa1152910:CDS 40.0%
!! GTCAAAGCTTTTGCTTTTGG+AGG + chr7_4:38955341-38955360 Msa1152910:CDS 40.0%
TGGCATAGAGAGTAATCTTG+AGG + chr7_4:38955371-38955390 Msa1152910:CDS 40.0%
TTTCAAGCTGTTGCTGTAGT+CGG + chr7_4:38955426-38955445 Msa1152910:CDS 40.0%
GTTGCTTATGAGGAAATTGC+TGG + chr7_4:38955683-38955702 Msa1152910:CDS 40.0%
TAAGTTGCAGAGAAAAGCTG+AGG + chr7_4:38955737-38955756 Msa1152910:CDS 40.0%
!! CAAGGAAAACTGCTCTAAAG+AGG - chr7_4:38955962-38955981 None:intergenic 40.0%
! CAGTTTTCCTTGATGCACTA+TGG + chr7_4:38955970-38955989 Msa1152910:intron 40.0%
TATTGAGCCATAGTGCATCA+AGG - chr7_4:38955980-38955999 None:intergenic 40.0%
ACTTGACCATCAAGAACCAT+TGG - chr7_4:38956443-38956462 None:intergenic 40.0%
! TTCCTGCTTTCAACCTTTTG+AGG + chr7_4:38956518-38956537 Msa1152910:intron 40.0%
CTTGAGATGTGTCTGATTAG+CGG + chr7_4:38956817-38956836 Msa1152910:intron 40.0%
AATCTACTTGTGTTGGGAGT+TGG + chr7_4:38956883-38956902 Msa1152910:intron 40.0%
! ATGTTTCTATAAGGGTAGGC+GGG - chr7_4:38957170-38957189 None:intergenic 40.0%
GCCTACCCTTATAGAAACAT+TGG + chr7_4:38957170-38957189 Msa1152910:exon 40.0%
CAAAGTCAAAGGTCTCCAAT+TGG - chr7_4:38957217-38957236 None:intergenic 40.0%
! CAATTGGAGACCTTTGACTT+TGG + chr7_4:38957215-38957234 Msa1152910:three_prime_UTR 40.0%
GCTTACCATTCCAAAGTCAA+AGG - chr7_4:38957228-38957247 None:intergenic 40.0%
CTTGAGTAACGTGTAGTAGA+TGG - chr7_4:38957287-38957306 None:intergenic 40.0%
GGTCCTCAAATGAAGAATCA+AGG - chr7_4:38957385-38957404 None:intergenic 40.0%
GATACTCTTGCCCAGAAAAA+TGG + chr7_4:38957494-38957513 Msa1152910:three_prime_UTR 40.0%
AAGTATGAGATGACGAGAGG+AGG + chr7_4:38953733-38953752 Msa1152910:five_prime_UTR 45.0%
CATTCGATATCCGCCTCATT+CGG - chr7_4:38953885-38953904 None:intergenic 45.0%
GAATGAGGCGGATATCGAAT+GGG + chr7_4:38953884-38953903 Msa1152910:CDS 45.0%
GATGTTGATCATCGGTCCAT+CGG - chr7_4:38953967-38953986 None:intergenic 45.0%
TCACAGAAAGAGGATTGAGC+TGG - chr7_4:38954029-38954048 None:intergenic 45.0%
GAAGAGAGTCTCACAGAAAG+AGG - chr7_4:38954039-38954058 None:intergenic 45.0%
TTAAAGGACTTAGCTCGCTC+AGG - chr7_4:38954667-38954686 None:intergenic 45.0%
TTCGAACTGAGGTGAACTGT+AGG + chr7_4:38954814-38954833 Msa1152910:intron 45.0%
GAATTTGCACATGGAAGGTG+TGG + chr7_4:38955311-38955330 Msa1152910:CDS 45.0%
!!! AATTTTGCAGGTGTTGGCCT+TGG + chr7_4:38955602-38955621 Msa1152910:intron 45.0%
TATGAGGAAATTGCTGGAGC+TGG + chr7_4:38955689-38955708 Msa1152910:CDS 45.0%
CTGGATGGTATTAAGGCTGA+AGG + chr7_4:38955708-38955727 Msa1152910:CDS 45.0%
TGGGTCAGAGAACGATAAGT+GGG + chr7_4:38956099-38956118 Msa1152910:intron 45.0%
GACCTCAAAAGGTTGAAAGC+AGG - chr7_4:38956523-38956542 None:intergenic 45.0%
CGCTAATCAGACACATCTCA+AGG - chr7_4:38956819-38956838 None:intergenic 45.0%
AATTCTTGTACTGCTTGCGG+TGG + chr7_4:38956995-38957014 Msa1152910:intron 45.0%
!! TTGCGGTGGACTTCACATTT+TGG + chr7_4:38957009-38957028 Msa1152910:intron 45.0%
AGTCTCAGCAACAACCCAAT+TGG + chr7_4:38957199-38957218 Msa1152910:three_prime_UTR 45.0%
! GGAGACCTTTGACTTTGGAA+TGG + chr7_4:38957220-38957239 Msa1152910:three_prime_UTR 45.0%
! GGTGATAATGACGATGAGGT+TGG - chr7_4:38957260-38957279 None:intergenic 45.0%
! TGGTGGTGATAATGACGATG+AGG - chr7_4:38957264-38957283 None:intergenic 45.0%
! TAACGTGTAGTAGATGGTGG+TGG - chr7_4:38957281-38957300 None:intergenic 45.0%
GAGTAACGTGTAGTAGATGG+TGG - chr7_4:38957284-38957303 None:intergenic 45.0%
ACGGGGAAAGACAAGAGAAG+AGG - chr7_4:38953777-38953796 None:intergenic 50.0%
GAATAAGCGGCGGTGTTCAT+CGG - chr7_4:38953846-38953865 None:intergenic 50.0%
AACACCGCCGCTTATTCTTC+CGG + chr7_4:38953849-38953868 Msa1152910:CDS 50.0%
CGAGACACCGGAAGAATAAG+CGG - chr7_4:38953859-38953878 None:intergenic 50.0%
CGAATGAGGCGGATATCGAA+TGG + chr7_4:38953883-38953902 Msa1152910:CDS 50.0%
TGTCTGTACGAACATACCGC+CGG - chr7_4:38953919-38953938 None:intergenic 50.0%
TTCGTACAGACACGCGAATC+AGG + chr7_4:38953927-38953946 Msa1152910:CDS 50.0%
GGTGACGATGATCATACCGA+TGG + chr7_4:38953948-38953967 Msa1152910:CDS 50.0%
! TTTGCACATGGAAGGTGTGG+AGG + chr7_4:38955314-38955333 Msa1152910:CDS 50.0%
!! TTGCTTTTGGAGGATGCTGC+TGG + chr7_4:38955351-38955370 Msa1152910:CDS 50.0%
TGTTGCTGTAGTCGGATCAG+AGG + chr7_4:38955434-38955453 Msa1152910:CDS 50.0%
! AGGTCGACAAGCTTTGTGAC+AGG + chr7_4:38955454-38955473 Msa1152910:CDS 50.0%
! GGTCGACAAGCTTTGTGACA+GGG + chr7_4:38955455-38955474 Msa1152910:CDS 50.0%
TCCATTGACAGCCACATCCA+AGG - chr7_4:38955622-38955641 None:intergenic 50.0%
TTGGATGTGGCTGTCAATGG+AGG + chr7_4:38955621-38955640 Msa1152910:CDS 50.0%
TGGATGTGGCTGTCAATGGA+GGG + chr7_4:38955622-38955641 Msa1152910:CDS 50.0%
AGGAAATTGCTGGAGCTGGA+TGG + chr7_4:38955693-38955712 Msa1152910:CDS 50.0%
GCTGGAGCTGGATGGTATTA+AGG + chr7_4:38955701-38955720 Msa1152910:CDS 50.0%
CTGGGTCAGAGAACGATAAG+TGG + chr7_4:38956098-38956117 Msa1152910:intron 50.0%
TACACGACGCACCTGTTTGA+AGG - chr7_4:38957104-38957123 None:intergenic 50.0%
!!! GCGGGAGTTTTTTGCCTTCA+GGG - chr7_4:38957152-38957171 None:intergenic 50.0%
GACACCGGAAGAATAAGCGG+CGG - chr7_4:38953856-38953875 None:intergenic 55.0%
TCGATATCCGCCTCATTCGG+CGG - chr7_4:38953882-38953901 None:intergenic 55.0%
ATCGAATGGGAGATGCGTCC+CGG + chr7_4:38953897-38953916 Msa1152910:CDS 55.0%
GTCTGTACGAACATACCGCC+GGG - chr7_4:38953918-38953937 None:intergenic 55.0%
GCCTTGGATGTGGCTGTCAA+TGG + chr7_4:38955618-38955637 Msa1152910:CDS 55.0%
!!! GGCGGGAGTTTTTTGCCTTC+AGG - chr7_4:38957153-38957172 None:intergenic 55.0%
!! GCAGGTGTTGGCCTTGGATG+TGG + chr7_4:38955608-38955627 Msa1152910:intron 60.0%
GGCTGTCAATGGAGGGACCA+AGG + chr7_4:38955629-38955648 Msa1152910:CDS 60.0%
CTCATTCGGCGGCGAGACAC+CGG - chr7_4:38953871-38953890 None:intergenic 65.0%
TGTCTCGCCGCCGAATGAGG+CGG + chr7_4:38953872-38953891 Msa1152910:CDS 65.0%
GAATGGGAGATGCGTCCCGG+CGG + chr7_4:38953900-38953919 Msa1152910:CDS 65.0%
CGGTGTCTCGCCGCCGAATG+AGG + chr7_4:38953869-38953888 Msa1152910:CDS 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 38953670 38957666 38953670 ID=Msa1152910;Name=Msa1152910
chr7_4 mRNA 38953670 38957666 38953670 ID=Msa1152910-mRNA-1;Parent=Msa1152910;Name=Msa1152910-mRNA-1;_AED=0.21;_eAED=0.21;_QI=176|1|1|1|1|1|4|483|224
chr7_4 exon 38953670 38954044 38953670 ID=Msa1152910-mRNA-1:exon:9425;Parent=Msa1152910-mRNA-1
chr7_4 exon 38955220 38955476 38955220 ID=Msa1152910-mRNA-1:exon:9426;Parent=Msa1152910-mRNA-1
chr7_4 exon 38955612 38955758 38955612 ID=Msa1152910-mRNA-1:exon:9427;Parent=Msa1152910-mRNA-1
chr7_4 exon 38957112 38957666 38957112 ID=Msa1152910-mRNA-1:exon:9428;Parent=Msa1152910-mRNA-1
chr7_4 five_prime_UTR 38953670 38953845 38953670 ID=Msa1152910-mRNA-1:five_prime_utr;Parent=Msa1152910-mRNA-1
chr7_4 CDS 38953846 38954044 38953846 ID=Msa1152910-mRNA-1:cds;Parent=Msa1152910-mRNA-1
chr7_4 CDS 38955220 38955476 38955220 ID=Msa1152910-mRNA-1:cds;Parent=Msa1152910-mRNA-1
chr7_4 CDS 38955612 38955758 38955612 ID=Msa1152910-mRNA-1:cds;Parent=Msa1152910-mRNA-1
chr7_4 CDS 38957112 38957183 38957112 ID=Msa1152910-mRNA-1:cds;Parent=Msa1152910-mRNA-1
chr7_4 three_prime_UTR 38957184 38957666 38957184 ID=Msa1152910-mRNA-1:three_prime_utr;Parent=Msa1152910-mRNA-1
Gene Sequence

>Msa1152910

ATGAACACCGCCGCTTATTCTTCCGGTGTCTCGCCGCCGAATGAGGCGGATATCGAATGGGAGATGCGTCCCGGCGGTATGTTCGTACAGACACGCGAATCAGGTGACGATGATCATACCGATGGACCGATGATCAACATCAGTGTTGCGTATGGTTCTTCTAATCACGAAGTTTATCTTCCAGCTCAATCCTCTTTCTGGGATGTTAAGAAACTGCTTGCACACAAAACTGGTTTAAAGCCTGAACAGCAAATACTCTTCTTTAAAGGAAAAGAAAAGGAGAATGAAGAGAATTTGCACATGGAAGGTGTGGAGGACAAGTCAAAGCTTTTGCTTTTGGAGGATGCTGCTGGCATAGAGAGTAATCTTGAGGAAGTTAGAAAACAAAATGAAATGTTAAAAGCTTTTCAAGCTGTTGCTGTAGTCGGATCAGAGGTCGACAAGCTTTGTGACAGGGTGTTGGCCTTGGATGTGGCTGTCAATGGAGGGACCAAGGTTTCTGACAAAGAATTTATTGTTACTACAGAGTTGCTTATGAGGAAATTGCTGGAGCTGGATGGTATTAAGGCTGAAGGTGAAGCTAAGTTGCAGAGAAAAGCTGAGGTGCGTCGTGTACAGAACGCTGTTGATACTTTAGATTCCCTGAAGGCAAAAAACTCCCGCCTACCCTTATAG
Protein sequence

>Msa1152910

MNTAAYSSGVSPPNEADIEWEMRPGGMFVQTRESGDDDHTDGPMINISVAYGSSNHEVYLPAQSSFWDVKKLLAHKTGLKPEQQILFFKGKEKENEENLHMEGVEDKSKLLLLEDAAGIESNLEEVRKQNEMLKAFQAVAVVGSEVDKLCDRVLALDVAVNGGTKVSDKEFIVTTELLMRKLLELDGIKAEGEAKLQRKAEVRRVQNAVDTLDSLKAKNSRLPL*