AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1156380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1156380 MtrunA17_Chr7g0250191 41.784 213 114 8 119 327 1 207 2.05e-35 128
Msa1156380 MtrunA17_Chr7g0250171 40.104 192 105 5 121 307 92 278 1.60e-30 117
Msa1156380 MtrunA17_Chr7g0225921 44.444 126 62 5 1 119 1 125 4.66e-18 79.7
Msa1156380 MtrunA17_Chr6g0459231 44.444 90 47 2 1 88 3 91 1.27e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATATGCTACCTTATAAAT+TGG 0.194854 7_4:-44661855 Msa1156380:CDS
GGGTTTCTACTGACATTATT+TGG 0.199993 7_4:-44662030 Msa1156380:CDS
GTACTGTTTGTGGTTATTTC+AGG 0.218791 7_4:+44661952 None:intergenic
TTCAGTTACTTTGATGTAAA+TGG 0.224758 7_4:-44662726 Msa1156380:CDS
GCGGTGATTTGCGCAATTTC+TGG 0.233947 7_4:+44661883 None:intergenic
AAGCTGATGCCTTGCGTAAC+AGG 0.279171 7_4:+44661749 None:intergenic
TTTGTAACTTCAGTGGCTTT+TGG 0.337238 7_4:-44661673 Msa1156380:CDS
CAACTAGAATCCAATTTATA+AGG 0.347305 7_4:+44661845 None:intergenic
AGTCTCTGTAGCATGCTTGC+TGG 0.352137 7_4:-44662310 Msa1156380:CDS
CCCATGTTCGCGTGGCGAAA+TGG 0.354518 7_4:+44662050 None:intergenic
CTTGCTCCTCTTCAGGCAAA+TGG 0.379382 7_4:-44662174 Msa1156380:intron
CGAGGGAACAAGGAAGATAA+AGG 0.383866 7_4:-44662555 Msa1156380:CDS
TGTACCATTCCTCTACTTCC+CGG 0.384557 7_4:-44661773 Msa1156380:CDS
GTTTGTGGTTATTTCAGGTT+GGG 0.384684 7_4:+44661957 None:intergenic
CATTGCTCGTTCTTTATGTC+AGG 0.401081 7_4:-44661624 Msa1156380:CDS
TCCATTTCGCCACGCGAACA+TGG 0.411223 7_4:-44662051 Msa1156380:CDS
GTTATCTGTGGAGGATTTAG+AGG 0.411714 7_4:-44662646 Msa1156380:CDS
GTCTCTGTAGCATGCTTGCT+GGG 0.411876 7_4:-44662309 Msa1156380:CDS
TCATCTTGAGTACTTATTGT+TGG 0.412372 7_4:+44661583 None:intergenic
GCTAAATTGTTAGAAGATAC+AGG 0.420133 7_4:+44662132 None:intergenic
TACTCAAGATGAGGTTGTCC+TGG 0.433902 7_4:-44661572 Msa1156380:CDS
ACTCCTAAGAAAATTCTTCA+AGG 0.447232 7_4:+44662080 None:intergenic
TAAGCACGGTGTAATTATAC+AGG 0.447737 7_4:-44661440 Msa1156380:three_prime_UTR
TAAGCACGGTGTAATTATAC+AGG 0.447737 7_4:-44661517 Msa1156380:three_prime_UTR
CTAGACGGTGATGAACTGTT+GGG 0.455821 7_4:-44662609 Msa1156380:CDS
TGTTTGTGGTTATTTCAGGT+TGG 0.464613 7_4:+44661956 None:intergenic
GAAGTTACAAATGCAATAAG+AGG 0.465364 7_4:+44661685 None:intergenic
TATTGCATTTGTAACTTCAG+TGG 0.472514 7_4:-44661680 Msa1156380:CDS
TCAGGTTGGGACTGATCAAA+TGG 0.477463 7_4:+44661970 None:intergenic
CCATTTCGCCACGCGAACAT+GGG 0.483035 7_4:-44662050 Msa1156380:CDS
ATGCCTTGCGTAACAGGTCC+GGG 0.487951 7_4:+44661755 None:intergenic
GATGCTTCATGAGGTTATCC+TGG 0.494541 7_4:-44661495 Msa1156380:three_prime_UTR
TAAAGGGCAAAGCTTTAAAG+AGG 0.495939 7_4:-44662538 Msa1156380:CDS
CTTACAGGTTTCCGTTCCAC+CGG 0.510555 7_4:-44662374 Msa1156380:intron
CGGAGATTTGTGGAAGCTCG+AGG 0.524244 7_4:-44662573 Msa1156380:CDS
CCTCCACAGATAACTCCTCA+TGG 0.528422 7_4:+44662655 None:intergenic
TCTAGACGGTGATGAACTGT+TGG 0.531424 7_4:-44662610 Msa1156380:CDS
GGAGGATTTAGAGGCGGTTG+AGG 0.533575 7_4:-44662637 Msa1156380:CDS
TGAGTGTGGTGTACTGTTTG+TGG 0.544187 7_4:+44661942 None:intergenic
CGTTCAAATCATCCTCATCC+AGG 0.544881 7_4:+44661554 None:intergenic
CTTTATGTCAGGTTACTCCA+TGG 0.545073 7_4:-44661613 Msa1156380:CDS
TGTTCAAATCATCCTCATCC+AGG 0.548909 7_4:+44661477 None:intergenic
GAGGGAACAAGGAAGATAAA+GGG 0.552009 7_4:-44662554 Msa1156380:CDS
TTACGCGTTCATGTGGCTTG+CGG 0.554497 7_4:-44661727 Msa1156380:CDS
TGTGGAAGCTCGAGGGAACA+AGG 0.565881 7_4:-44662565 Msa1156380:CDS
ATCAGCTTTACGCGTTCATG+TGG 0.567312 7_4:-44661734 Msa1156380:CDS
TGCCACTAGCACTTAGACTG+CGG 0.573207 7_4:-44661822 Msa1156380:CDS
AACAATAAGTACTCAAGATG+AGG 0.587586 7_4:-44661581 Msa1156380:CDS
CACAGATAACTCCTCATGGA+TGG 0.590113 7_4:+44662659 None:intergenic
AGATACAGGAAGAACATCGT+TGG 0.591251 7_4:+44662146 None:intergenic
TAGACGGTGATGAACTGTTG+GGG 0.595417 7_4:-44662608 Msa1156380:CDS
TGAATTGCAAAATTGCATGA+AGG 0.597499 7_4:-44662682 Msa1156380:CDS
AGATGAGGTTGTCCTGGATG+AGG 0.599334 7_4:-44661566 Msa1156380:CDS
TCATGAGGTTATCCTGGATG+AGG 0.601123 7_4:-44661489 Msa1156380:three_prime_UTR
TTACAGGTTTCCGTTCCACC+GGG 0.603359 7_4:-44662373 Msa1156380:intron
CGGAAGCTTACTAGTGAGTG+TGG 0.610294 7_4:+44661928 None:intergenic
GGAGATTTGTGGAAGCTCGA+GGG 0.611100 7_4:-44662572 Msa1156380:CDS
GCGGTTGAGGCATTTCTAGA+CGG 0.612643 7_4:-44662624 Msa1156380:CDS
ATTATACAGGATGCTTCATG+AGG 0.626242 7_4:-44661504 Msa1156380:three_prime_UTR
ATATATGGTTATTATGGACA+AGG 0.627190 7_4:-44662769 None:intergenic
ATCTGTGGAGGATTTAGAGG+CGG 0.627233 7_4:-44662643 Msa1156380:CDS
CAAATGCAATAAGAGGCAGC+TGG 0.632451 7_4:+44661692 None:intergenic
CAGATGTCTTCGTTTAAGCA+CGG 0.633417 7_4:-44661454 Msa1156380:three_prime_UTR
GTAACAGGTCCGGGAAGTAG+AGG 0.634533 7_4:+44661764 None:intergenic
AGGTCCGGGAAGTAGAGGAA+TGG 0.634665 7_4:+44661769 None:intergenic
AATGGTACATAAAACAAGAG+TGG 0.636670 7_4:+44661787 None:intergenic
CAGATGTCTTCGTTTAAGCA+CGG 0.638065 7_4:-44661531 Msa1156380:exon
AGTAGAAACCCATGTTCGCG+TGG 0.639401 7_4:+44662042 None:intergenic
AGCCGCAGTCTAAGTGCTAG+TGG 0.639546 7_4:+44661820 None:intergenic
CCATGAGGAGTTATCTGTGG+AGG 0.646843 7_4:-44662655 Msa1156380:CDS
CTTCCCGGACCTGTTACGCA+AGG 0.650567 7_4:-44661758 Msa1156380:CDS
GGTACATACATAGAAGAGCA+AGG 0.665141 7_4:+44662279 None:intergenic
CATCCATGAGGAGTTATCTG+TGG 0.683615 7_4:-44662658 Msa1156380:CDS
TTGGGACTGATCAAATGGAG+TGG 0.687424 7_4:+44661975 None:intergenic
AAGGTAGCATATCATGAATG+CGG 0.687795 7_4:+44661864 None:intergenic
TTGCATGAAGGCCATCCATG+AGG 0.708194 7_4:-44662670 Msa1156380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAGAGGTTTTTAATGTTT+TGG - chr7_4:44661595-44661614 Msa1156380:CDS 20.0%
!!! TAAAGAGGTTTTTAATGTTT+TGG - chr7_4:44661595-44661614 Msa1156380:CDS 20.0%
!! TTCAGTTACTTTGATGTAAA+TGG - chr7_4:44661392-44661411 Msa1156380:three_prime_UTR 25.0%
!!! GTTTTTTTATAAACGCTTAC+AGG - chr7_4:44661729-44661748 Msa1156380:CDS 25.0%
!! GTGATAAAAAATTTGAGACA+CGG - chr7_4:44661767-44661786 Msa1156380:CDS 25.0%
!! TGATAAAAAATTTGAGACAC+GGG - chr7_4:44661768-44661787 Msa1156380:CDS 25.0%
! TGATATGCTACCTTATAAAT+TGG - chr7_4:44662263-44662282 Msa1156380:intron 25.0%
! CAACTAGAATCCAATTTATA+AGG + chr7_4:44662276-44662295 None:intergenic 25.0%
!! TTCAGTTACTTTGATGTAAA+TGG - chr7_4:44661392-44661411 Msa1156380:three_prime_UTR 25.0%
!!! GTTTTTTTATAAACGCTTAC+AGG - chr7_4:44661729-44661748 Msa1156380:CDS 25.0%
!! GTGATAAAAAATTTGAGACA+CGG - chr7_4:44661767-44661786 Msa1156380:CDS 25.0%
!! TGATAAAAAATTTGAGACAC+GGG - chr7_4:44661768-44661787 Msa1156380:CDS 25.0%
! TGATATGCTACCTTATAAAT+TGG - chr7_4:44662263-44662282 Msa1156380:intron 25.0%
! CAACTAGAATCCAATTTATA+AGG + chr7_4:44662276-44662295 None:intergenic 25.0%
TGAATTGCAAAATTGCATGA+AGG - chr7_4:44661436-44661455 Msa1156380:three_prime_UTR 30.0%
!! TTTTAATGTTTTGGATCCTG+AGG - chr7_4:44661604-44661623 Msa1156380:CDS 30.0%
AATTAATATGCCTCTTGAGA+TGG + chr7_4:44661649-44661668 None:intergenic 30.0%
!!! GTCTCAAATTTTTTATCACC+CGG + chr7_4:44661766-44661785 None:intergenic 30.0%
GCTAAATTGTTAGAAGATAC+AGG + chr7_4:44661989-44662008 None:intergenic 30.0%
! TCACCTTGAAGAATTTTCTT+AGG - chr7_4:44662035-44662054 Msa1156380:CDS 30.0%
ACTCCTAAGAAAATTCTTCA+AGG + chr7_4:44662041-44662060 None:intergenic 30.0%
AGTAACCAAAAATTGCTTCA+CGG + chr7_4:44662213-44662232 None:intergenic 30.0%
AATGGTACATAAAACAAGAG+TGG + chr7_4:44662334-44662353 None:intergenic 30.0%
GAAGTTACAAATGCAATAAG+AGG + chr7_4:44662436-44662455 None:intergenic 30.0%
TATTGCATTTGTAACTTCAG+TGG - chr7_4:44662438-44662457 Msa1156380:intron 30.0%
!! TTATTGTTGGTGTTTTTCCA+TGG + chr7_4:44662525-44662544 None:intergenic 30.0%
TCATCTTGAGTACTTATTGT+TGG + chr7_4:44662538-44662557 None:intergenic 30.0%
AACAATAAGTACTCAAGATG+AGG - chr7_4:44662537-44662556 Msa1156380:CDS 30.0%
TGAATTGCAAAATTGCATGA+AGG - chr7_4:44661436-44661455 Msa1156380:three_prime_UTR 30.0%
!! TTTTAATGTTTTGGATCCTG+AGG - chr7_4:44661604-44661623 Msa1156380:CDS 30.0%
AATTAATATGCCTCTTGAGA+TGG + chr7_4:44661649-44661668 None:intergenic 30.0%
!!! GTCTCAAATTTTTTATCACC+CGG + chr7_4:44661766-44661785 None:intergenic 30.0%
GCTAAATTGTTAGAAGATAC+AGG + chr7_4:44661989-44662008 None:intergenic 30.0%
! TCACCTTGAAGAATTTTCTT+AGG - chr7_4:44662035-44662054 Msa1156380:CDS 30.0%
ACTCCTAAGAAAATTCTTCA+AGG + chr7_4:44662041-44662060 None:intergenic 30.0%
AGTAACCAAAAATTGCTTCA+CGG + chr7_4:44662213-44662232 None:intergenic 30.0%
AATGGTACATAAAACAAGAG+TGG + chr7_4:44662334-44662353 None:intergenic 30.0%
GAAGTTACAAATGCAATAAG+AGG + chr7_4:44662436-44662455 None:intergenic 30.0%
TATTGCATTTGTAACTTCAG+TGG - chr7_4:44662438-44662457 Msa1156380:intron 30.0%
!! TTATTGTTGGTGTTTTTCCA+TGG + chr7_4:44662525-44662544 None:intergenic 30.0%
TCATCTTGAGTACTTATTGT+TGG + chr7_4:44662538-44662557 None:intergenic 30.0%
AACAATAAGTACTCAAGATG+AGG - chr7_4:44662537-44662556 Msa1156380:CDS 30.0%
!! TGTTGGGGTTTGATGATTTT+CGG - chr7_4:44661525-44661544 Msa1156380:exon 35.0%
! TGATGATTTTCGGAGATTTG+TGG - chr7_4:44661535-44661554 Msa1156380:exon 35.0%
!! TAAAGGGCAAAGCTTTAAAG+AGG - chr7_4:44661580-44661599 Msa1156380:CDS 35.0%
!!! TCAAATTTTTTATCACCCGG+TGG + chr7_4:44661763-44661782 None:intergenic 35.0%
CAACATATCAAGCTAGCAAT+TGG + chr7_4:44661863-44661882 None:intergenic 35.0%
! TTTATTTCTTGCTCCTCTTC+AGG - chr7_4:44661937-44661956 Msa1156380:CDS 35.0%
! TGAAAGCTTTTTGTTCGAGT+GGG + chr7_4:44662018-44662037 None:intergenic 35.0%
GGGTTTCTACTGACATTATT+TGG - chr7_4:44662088-44662107 Msa1156380:CDS 35.0%
!! GTTTGTGGTTATTTCAGGTT+GGG + chr7_4:44662164-44662183 None:intergenic 35.0%
! TGTTTGTGGTTATTTCAGGT+TGG + chr7_4:44662165-44662184 None:intergenic 35.0%
! GTACTGTTTGTGGTTATTTC+AGG + chr7_4:44662169-44662188 None:intergenic 35.0%
AAGGTAGCATATCATGAATG+CGG + chr7_4:44662257-44662276 None:intergenic 35.0%
! TTTGTAACTTCAGTGGCTTT+TGG - chr7_4:44662445-44662464 Msa1156380:intron 35.0%
!!! CATTACCCTTGTTTTTGTCT+TGG - chr7_4:44662468-44662487 Msa1156380:intron 35.0%
TGTCACCAAGACAAAAACAA+GGG + chr7_4:44662476-44662495 None:intergenic 35.0%
ATGTCACCAAGACAAAAACA+AGG + chr7_4:44662477-44662496 None:intergenic 35.0%
TAAGCACGGTGTAATTATAC+AGG - chr7_4:44662601-44662620 Msa1156380:CDS 35.0%
ATTATACAGGATGCTTCATG+AGG - chr7_4:44662614-44662633 Msa1156380:CDS 35.0%
TAAGCACGGTGTAATTATAC+AGG - chr7_4:44662601-44662620 Msa1156380:CDS 35.0%
!! TGTTGGGGTTTGATGATTTT+CGG - chr7_4:44661525-44661544 Msa1156380:exon 35.0%
! TGATGATTTTCGGAGATTTG+TGG - chr7_4:44661535-44661554 Msa1156380:exon 35.0%
!! TAAAGGGCAAAGCTTTAAAG+AGG - chr7_4:44661580-44661599 Msa1156380:CDS 35.0%
!!! TCAAATTTTTTATCACCCGG+TGG + chr7_4:44661763-44661782 None:intergenic 35.0%
CAACATATCAAGCTAGCAAT+TGG + chr7_4:44661863-44661882 None:intergenic 35.0%
! TTTATTTCTTGCTCCTCTTC+AGG - chr7_4:44661937-44661956 Msa1156380:CDS 35.0%
! TGAAAGCTTTTTGTTCGAGT+GGG + chr7_4:44662018-44662037 None:intergenic 35.0%
GGGTTTCTACTGACATTATT+TGG - chr7_4:44662088-44662107 Msa1156380:CDS 35.0%
!! GTTTGTGGTTATTTCAGGTT+GGG + chr7_4:44662164-44662183 None:intergenic 35.0%
! TGTTTGTGGTTATTTCAGGT+TGG + chr7_4:44662165-44662184 None:intergenic 35.0%
! GTACTGTTTGTGGTTATTTC+AGG + chr7_4:44662169-44662188 None:intergenic 35.0%
AAGGTAGCATATCATGAATG+CGG + chr7_4:44662257-44662276 None:intergenic 35.0%
! TTTGTAACTTCAGTGGCTTT+TGG - chr7_4:44662445-44662464 Msa1156380:intron 35.0%
!!! CATTACCCTTGTTTTTGTCT+TGG - chr7_4:44662468-44662487 Msa1156380:intron 35.0%
TGTCACCAAGACAAAAACAA+GGG + chr7_4:44662476-44662495 None:intergenic 35.0%
ATGTCACCAAGACAAAAACA+AGG + chr7_4:44662477-44662496 None:intergenic 35.0%
TAAGCACGGTGTAATTATAC+AGG - chr7_4:44662601-44662620 Msa1156380:CDS 35.0%
ATTATACAGGATGCTTCATG+AGG - chr7_4:44662614-44662633 Msa1156380:CDS 35.0%
TAAGCACGGTGTAATTATAC+AGG - chr7_4:44662601-44662620 Msa1156380:CDS 35.0%
GTTATCTGTGGAGGATTTAG+AGG - chr7_4:44661472-44661491 Msa1156380:three_prime_UTR 40.0%
GAGGGAACAAGGAAGATAAA+GGG - chr7_4:44661564-44661583 Msa1156380:CDS 40.0%
TGATCAAATAGACTCACCTC+AGG + chr7_4:44661623-44661642 None:intergenic 40.0%
TTGATCAGTTCCATCTCAAG+AGG - chr7_4:44661636-44661655 Msa1156380:CDS 40.0%
!!! TTTTTTATCACCCGGTGGAA+CGG + chr7_4:44661758-44661777 None:intergenic 40.0%
GGTACATACATAGAAGAGCA+AGG + chr7_4:44661842-44661861 None:intergenic 40.0%
AGATACAGGAAGAACATCGT+TGG + chr7_4:44661975-44661994 None:intergenic 40.0%
! GAAAGCTTTTTGTTCGAGTG+GGG + chr7_4:44662017-44662036 None:intergenic 40.0%
! GTGAAAGCTTTTTGTTCGAG+TGG + chr7_4:44662019-44662038 None:intergenic 40.0%
! AGCTTCCGTGAAGCAATTTT+TGG - chr7_4:44662205-44662224 Msa1156380:intron 40.0%
CATTGCTCGTTCTTTATGTC+AGG - chr7_4:44662494-44662513 Msa1156380:intron 40.0%
CTTTATGTCAGGTTACTCCA+TGG - chr7_4:44662505-44662524 Msa1156380:intron 40.0%
CAGATGTCTTCGTTTAAGCA+CGG - chr7_4:44662587-44662606 Msa1156380:CDS 40.0%
TGTTCAAATCATCCTCATCC+AGG + chr7_4:44662644-44662663 None:intergenic 40.0%
CAGATGTCTTCGTTTAAGCA+CGG - chr7_4:44662587-44662606 Msa1156380:CDS 40.0%
GTTATCTGTGGAGGATTTAG+AGG - chr7_4:44661472-44661491 Msa1156380:three_prime_UTR 40.0%
GAGGGAACAAGGAAGATAAA+GGG - chr7_4:44661564-44661583 Msa1156380:CDS 40.0%
TGATCAAATAGACTCACCTC+AGG + chr7_4:44661623-44661642 None:intergenic 40.0%
TTGATCAGTTCCATCTCAAG+AGG - chr7_4:44661636-44661655 Msa1156380:CDS 40.0%
!!! TTTTTTATCACCCGGTGGAA+CGG + chr7_4:44661758-44661777 None:intergenic 40.0%
GGTACATACATAGAAGAGCA+AGG + chr7_4:44661842-44661861 None:intergenic 40.0%
AGATACAGGAAGAACATCGT+TGG + chr7_4:44661975-44661994 None:intergenic 40.0%
! GAAAGCTTTTTGTTCGAGTG+GGG + chr7_4:44662017-44662036 None:intergenic 40.0%
! GTGAAAGCTTTTTGTTCGAG+TGG + chr7_4:44662019-44662038 None:intergenic 40.0%
! AGCTTCCGTGAAGCAATTTT+TGG - chr7_4:44662205-44662224 Msa1156380:intron 40.0%
CATTGCTCGTTCTTTATGTC+AGG - chr7_4:44662494-44662513 Msa1156380:intron 40.0%
CTTTATGTCAGGTTACTCCA+TGG - chr7_4:44662505-44662524 Msa1156380:intron 40.0%
CAGATGTCTTCGTTTAAGCA+CGG - chr7_4:44662587-44662606 Msa1156380:CDS 40.0%
TGTTCAAATCATCCTCATCC+AGG + chr7_4:44662644-44662663 None:intergenic 40.0%
CAGATGTCTTCGTTTAAGCA+CGG - chr7_4:44662587-44662606 Msa1156380:CDS 40.0%
CACAGATAACTCCTCATGGA+TGG + chr7_4:44661462-44661481 None:intergenic 45.0%
CATCCATGAGGAGTTATCTG+TGG - chr7_4:44661460-44661479 Msa1156380:three_prime_UTR 45.0%
ATCTGTGGAGGATTTAGAGG+CGG - chr7_4:44661475-44661494 Msa1156380:three_prime_UTR 45.0%
TCTAGACGGTGATGAACTGT+TGG - chr7_4:44661508-44661527 Msa1156380:three_prime_UTR 45.0%
CTAGACGGTGATGAACTGTT+GGG - chr7_4:44661509-44661528 Msa1156380:three_prime_UTR 45.0%
TAGACGGTGATGAACTGTTG+GGG - chr7_4:44661510-44661529 Msa1156380:three_prime_UTR 45.0%
CGAGGGAACAAGGAAGATAA+AGG - chr7_4:44661563-44661582 Msa1156380:CDS 45.0%
GTTTCTCCATTTGCCTGAAG+AGG + chr7_4:44661953-44661972 None:intergenic 45.0%
TTGGGACTGATCAAATGGAG+TGG + chr7_4:44662146-44662165 None:intergenic 45.0%
!! TCAGGTTGGGACTGATCAAA+TGG + chr7_4:44662151-44662170 None:intergenic 45.0%
TGAGTGTGGTGTACTGTTTG+TGG + chr7_4:44662179-44662198 None:intergenic 45.0%
TGTACCATTCCTCTACTTCC+CGG - chr7_4:44662345-44662364 Msa1156380:CDS 45.0%
ATCAGCTTTACGCGTTCATG+TGG - chr7_4:44662384-44662403 Msa1156380:intron 45.0%
CAAATGCAATAAGAGGCAGC+TGG + chr7_4:44662429-44662448 None:intergenic 45.0%
TACTCAAGATGAGGTTGTCC+TGG - chr7_4:44662546-44662565 Msa1156380:CDS 45.0%
CGTTCAAATCATCCTCATCC+AGG + chr7_4:44662567-44662586 None:intergenic 45.0%
GATGCTTCATGAGGTTATCC+TGG - chr7_4:44662623-44662642 Msa1156380:CDS 45.0%
TCATGAGGTTATCCTGGATG+AGG - chr7_4:44662629-44662648 Msa1156380:CDS 45.0%
CACAGATAACTCCTCATGGA+TGG + chr7_4:44661462-44661481 None:intergenic 45.0%
CATCCATGAGGAGTTATCTG+TGG - chr7_4:44661460-44661479 Msa1156380:three_prime_UTR 45.0%
ATCTGTGGAGGATTTAGAGG+CGG - chr7_4:44661475-44661494 Msa1156380:three_prime_UTR 45.0%
TCTAGACGGTGATGAACTGT+TGG - chr7_4:44661508-44661527 Msa1156380:three_prime_UTR 45.0%
CTAGACGGTGATGAACTGTT+GGG - chr7_4:44661509-44661528 Msa1156380:three_prime_UTR 45.0%
TAGACGGTGATGAACTGTTG+GGG - chr7_4:44661510-44661529 Msa1156380:three_prime_UTR 45.0%
CGAGGGAACAAGGAAGATAA+AGG - chr7_4:44661563-44661582 Msa1156380:CDS 45.0%
GTTTCTCCATTTGCCTGAAG+AGG + chr7_4:44661953-44661972 None:intergenic 45.0%
TTGGGACTGATCAAATGGAG+TGG + chr7_4:44662146-44662165 None:intergenic 45.0%
!! TCAGGTTGGGACTGATCAAA+TGG + chr7_4:44662151-44662170 None:intergenic 45.0%
TGAGTGTGGTGTACTGTTTG+TGG + chr7_4:44662179-44662198 None:intergenic 45.0%
TGTACCATTCCTCTACTTCC+CGG - chr7_4:44662345-44662364 Msa1156380:CDS 45.0%
ATCAGCTTTACGCGTTCATG+TGG - chr7_4:44662384-44662403 Msa1156380:intron 45.0%
CAAATGCAATAAGAGGCAGC+TGG + chr7_4:44662429-44662448 None:intergenic 45.0%
TACTCAAGATGAGGTTGTCC+TGG - chr7_4:44662546-44662565 Msa1156380:CDS 45.0%
CGTTCAAATCATCCTCATCC+AGG + chr7_4:44662567-44662586 None:intergenic 45.0%
GATGCTTCATGAGGTTATCC+TGG - chr7_4:44662623-44662642 Msa1156380:CDS 45.0%
TCATGAGGTTATCCTGGATG+AGG - chr7_4:44662629-44662648 Msa1156380:CDS 45.0%
TTGCATGAAGGCCATCCATG+AGG - chr7_4:44661448-44661467 Msa1156380:three_prime_UTR 50.0%
CCTCCACAGATAACTCCTCA+TGG + chr7_4:44661466-44661485 None:intergenic 50.0%
CCATGAGGAGTTATCTGTGG+AGG - chr7_4:44661463-44661482 Msa1156380:three_prime_UTR 50.0%
! GCGGTTGAGGCATTTCTAGA+CGG - chr7_4:44661494-44661513 Msa1156380:three_prime_UTR 50.0%
GGAGATTTGTGGAAGCTCGA+GGG - chr7_4:44661546-44661565 Msa1156380:CDS 50.0%
CTTACAGGTTTCCGTTCCAC+CGG - chr7_4:44661744-44661763 Msa1156380:CDS 50.0%
TTACAGGTTTCCGTTCCACC+GGG - chr7_4:44661745-44661764 Msa1156380:CDS 50.0%
AGTCTCTGTAGCATGCTTGC+TGG - chr7_4:44661808-44661827 Msa1156380:CDS 50.0%
GTCTCTGTAGCATGCTTGCT+GGG - chr7_4:44661809-44661828 Msa1156380:CDS 50.0%
CTTGCTCCTCTTCAGGCAAA+TGG - chr7_4:44661944-44661963 Msa1156380:CDS 50.0%
AGTAGAAACCCATGTTCGCG+TGG + chr7_4:44662079-44662098 None:intergenic 50.0%
CGGAAGCTTACTAGTGAGTG+TGG + chr7_4:44662193-44662212 None:intergenic 50.0%
! GCGGTGATTTGCGCAATTTC+TGG + chr7_4:44662238-44662257 None:intergenic 50.0%
TGCCACTAGCACTTAGACTG+CGG - chr7_4:44662296-44662315 Msa1156380:CDS 50.0%
AAGCTGATGCCTTGCGTAAC+AGG + chr7_4:44662372-44662391 None:intergenic 50.0%
TTACGCGTTCATGTGGCTTG+CGG - chr7_4:44662391-44662410 Msa1156380:intron 50.0%
AGATGAGGTTGTCCTGGATG+AGG - chr7_4:44662552-44662571 Msa1156380:CDS 50.0%
TTGCATGAAGGCCATCCATG+AGG - chr7_4:44661448-44661467 Msa1156380:three_prime_UTR 50.0%
CCTCCACAGATAACTCCTCA+TGG + chr7_4:44661466-44661485 None:intergenic 50.0%
CCATGAGGAGTTATCTGTGG+AGG - chr7_4:44661463-44661482 Msa1156380:three_prime_UTR 50.0%
! GCGGTTGAGGCATTTCTAGA+CGG - chr7_4:44661494-44661513 Msa1156380:three_prime_UTR 50.0%
GGAGATTTGTGGAAGCTCGA+GGG - chr7_4:44661546-44661565 Msa1156380:CDS 50.0%
CTTACAGGTTTCCGTTCCAC+CGG - chr7_4:44661744-44661763 Msa1156380:CDS 50.0%
TTACAGGTTTCCGTTCCACC+GGG - chr7_4:44661745-44661764 Msa1156380:CDS 50.0%
AGTCTCTGTAGCATGCTTGC+TGG - chr7_4:44661808-44661827 Msa1156380:CDS 50.0%
GTCTCTGTAGCATGCTTGCT+GGG - chr7_4:44661809-44661828 Msa1156380:CDS 50.0%
CTTGCTCCTCTTCAGGCAAA+TGG - chr7_4:44661944-44661963 Msa1156380:CDS 50.0%
AGTAGAAACCCATGTTCGCG+TGG + chr7_4:44662079-44662098 None:intergenic 50.0%
CGGAAGCTTACTAGTGAGTG+TGG + chr7_4:44662193-44662212 None:intergenic 50.0%
! GCGGTGATTTGCGCAATTTC+TGG + chr7_4:44662238-44662257 None:intergenic 50.0%
TGCCACTAGCACTTAGACTG+CGG - chr7_4:44662296-44662315 Msa1156380:CDS 50.0%
AAGCTGATGCCTTGCGTAAC+AGG + chr7_4:44662372-44662391 None:intergenic 50.0%
TTACGCGTTCATGTGGCTTG+CGG - chr7_4:44662391-44662410 Msa1156380:intron 50.0%
AGATGAGGTTGTCCTGGATG+AGG - chr7_4:44662552-44662571 Msa1156380:CDS 50.0%
GGAGGATTTAGAGGCGGTTG+AGG - chr7_4:44661481-44661500 Msa1156380:three_prime_UTR 55.0%
CGGAGATTTGTGGAAGCTCG+AGG - chr7_4:44661545-44661564 Msa1156380:CDS 55.0%
TGTGGAAGCTCGAGGGAACA+AGG - chr7_4:44661553-44661572 Msa1156380:CDS 55.0%
TCCATTTCGCCACGCGAACA+TGG - chr7_4:44662067-44662086 Msa1156380:CDS 55.0%
CCATTTCGCCACGCGAACAT+GGG - chr7_4:44662068-44662087 Msa1156380:CDS 55.0%
AGCCGCAGTCTAAGTGCTAG+TGG + chr7_4:44662301-44662320 None:intergenic 55.0%
AGGTCCGGGAAGTAGAGGAA+TGG + chr7_4:44662352-44662371 None:intergenic 55.0%
GTAACAGGTCCGGGAAGTAG+AGG + chr7_4:44662357-44662376 None:intergenic 55.0%
ATGCCTTGCGTAACAGGTCC+GGG + chr7_4:44662366-44662385 None:intergenic 55.0%
GATGCCTTGCGTAACAGGTC+CGG + chr7_4:44662367-44662386 None:intergenic 55.0%
GGAGGATTTAGAGGCGGTTG+AGG - chr7_4:44661481-44661500 Msa1156380:three_prime_UTR 55.0%
CGGAGATTTGTGGAAGCTCG+AGG - chr7_4:44661545-44661564 Msa1156380:CDS 55.0%
TGTGGAAGCTCGAGGGAACA+AGG - chr7_4:44661553-44661572 Msa1156380:CDS 55.0%
TCCATTTCGCCACGCGAACA+TGG - chr7_4:44662067-44662086 Msa1156380:CDS 55.0%
CCATTTCGCCACGCGAACAT+GGG - chr7_4:44662068-44662087 Msa1156380:CDS 55.0%
AGCCGCAGTCTAAGTGCTAG+TGG + chr7_4:44662301-44662320 None:intergenic 55.0%
AGGTCCGGGAAGTAGAGGAA+TGG + chr7_4:44662352-44662371 None:intergenic 55.0%
GTAACAGGTCCGGGAAGTAG+AGG + chr7_4:44662357-44662376 None:intergenic 55.0%
ATGCCTTGCGTAACAGGTCC+GGG + chr7_4:44662366-44662385 None:intergenic 55.0%
GATGCCTTGCGTAACAGGTC+CGG + chr7_4:44662367-44662386 None:intergenic 55.0%
CCCATGTTCGCGTGGCGAAA+TGG + chr7_4:44662071-44662090 None:intergenic 60.0%
CTTCCCGGACCTGTTACGCA+AGG - chr7_4:44662360-44662379 Msa1156380:CDS 60.0%
CCCATGTTCGCGTGGCGAAA+TGG + chr7_4:44662071-44662090 None:intergenic 60.0%
CTTCCCGGACCTGTTACGCA+AGG - chr7_4:44662360-44662379 Msa1156380:CDS 60.0%
! CGCGTTCATGTGGCTTGCGG+CGG - chr7_4:44662394-44662413 Msa1156380:intron 65.0%
! CGCGTTCATGTGGCTTGCGG+CGG - chr7_4:44662394-44662413 Msa1156380:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 44661362 44662778 44661362 ID=Msa1156380;Name=Msa1156380
chr7_4 mRNA 44661362 44662778 44661362 ID=Msa1156380-mRNA-1;Parent=Msa1156380;Name=Msa1156380-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|1|0.66|1|1|1|3|175|336
chr7_4 exon 44661362 44662181 44661362 ID=Msa1156380-mRNA-1:exon:11536;Parent=Msa1156380-mRNA-1
chr7_4 exon 44662288 44662389 44662288 ID=Msa1156380-mRNA-1:exon:11535;Parent=Msa1156380-mRNA-1
chr7_4 exon 44662515 44662778 44662515 ID=Msa1156380-mRNA-1:exon:11534;Parent=Msa1156380-mRNA-1
chr7_4 CDS 44662515 44662778 44662515 ID=Msa1156380-mRNA-1:cds;Parent=Msa1156380-mRNA-1
chr7_4 CDS 44662288 44662389 44662288 ID=Msa1156380-mRNA-1:cds;Parent=Msa1156380-mRNA-1
chr7_4 CDS 44661537 44662181 44661537 ID=Msa1156380-mRNA-1:cds;Parent=Msa1156380-mRNA-1
chr7_4 three_prime_UTR 44661362 44661536 44661362 ID=Msa1156380-mRNA-1:three_prime_utr;Parent=Msa1156380-mRNA-1
Gene Sequence

>Msa1156380

ATGGACAAGGTAATCGACTATGAACTTGTGTTCAGTTACTTTGATGTAAATGGAGATAGAAAGATTACGCTTGCTGAATTGCAAAATTGCATGAAGGCCATCCATGAGGAGTTATCTGTGGAGGATTTAGAGGCGGTTGAGGCATTTCTAGACGGTGATGAACTGTTGGGGTTTGATGATTTTCGGAGATTTGTGGAAGCTCGAGGGAACAAGGAAGATAAAGGGCAAAGCTTTAAAGAGGTTTTTAATGTTTTGGATCCTGAGGTTTCCGTTCCACCGGGTGATAAAAAATTTGAGACACGGGTCAAAAAACTTGAGATCAGTCTCTGTAGCATGCTTGCTGGGATGTTCACCTTGCTCTTCTATGCAAATGGAGAAACCAACGATGTTCTTCCTGTATCTTCTAACAATTTAGCAGATGCCCCACTCGAACAAAAAGCTTTCACCTTGAAGAATTTTCTTAGGAGTGTGCTCTCCATTTCGCCACGCGAACATGGGTTTCTACTGACATTATTTGGTTACATACTAAGTTACTTTACAAAAAACTCTACCACTCCATTTGATCAGTCCCAACCTGAAATAACCACAAACAGTACACCACACTCACTAGTAAGCTTCCGTGAAGCAATTTTTGGTTACTCACCAGAAATTGCGCAAATCACCGCATTCATGATATGCTACCTTATAAATTGGATTCTAGTTGTGCCACTAGCACTTAGACTGCGGCTTTTTCTGATACCACTCTTGTTTTATGTACCATTCCTCTACTTCCCGGACCTGTTACGCAAGGCATCAGCTTTACGCGTTCATGTGGCTTGCGGCGGCTCATTCAACCAGCTGCCTCTTATTGCATTTGTAACTTCAGTGGCTTTTGGCATTACCCTTGTTTTTGTCTTGGTGACATTGCTCGTTCTTTATGTCAGGTTACTCCATGGAAAAACACCAACAATAAGTACTCAAGATGAGGTTGTCCTGGATGAGGATGATTTGAACGCAGATGTCTTCGTTTAA
Protein sequence

>Msa1156380

MDKVIDYELVFSYFDVNGDRKITLAELQNCMKAIHEELSVEDLEAVEAFLDGDELLGFDDFRRFVEARGNKEDKGQSFKEVFNVLDPEVSVPPGDKKFETRVKKLEISLCSMLAGMFTLLFYANGETNDVLPVSSNNLADAPLEQKAFTLKNFLRSVLSISPREHGFLLTLFGYILSYFTKNSTTPFDQSQPEITTNSTPHSLVSFREAIFGYSPEIAQITAFMICYLINWILVVPLALRLRLFLIPLLFYVPFLYFPDLLRKASALRVHVACGGSFNQLPLIAFVTSVAFGITLVFVLVTLLVLYVRLLHGKTPTISTQDEVVLDEDDLNADVFV*