AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1164370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1164370 MtrunA17_Chr7g0267961 89.256 242 10 4 1 226 216 457 6.50e-151 427
Msa1164370 MtrunA17_Chr5g0395821 72.199 241 52 3 1 226 214 454 5.86e-124 358
Msa1164370 MtrunA17_Chr3g0098921 70.248 242 56 4 1 226 218 459 5.71e-119 346
Msa1164370 MtrunA17_Chr7g0228191 76.211 227 37 4 1 210 103 329 1.63e-116 335
Msa1164370 MtrunA17_Chr7g0235181 71.193 243 48 7 1 226 189 426 8.92e-113 329
Msa1164370 MtrunA17_Chr7g0233651 67.220 241 55 4 1 226 200 431 3.83e-107 315
Msa1164370 MtrunA17_Chr7g0234591 68.724 243 56 5 1 226 197 436 5.92e-107 315
Msa1164370 MtrunA17_Chr2g0330601 62.771 231 46 6 7 224 166 369 2.50e-90 270
Msa1164370 MtrunA17_Chr2g0330681 65.241 187 39 6 7 180 196 369 4.51e-70 218
Msa1164370 MtrunA17_Chr3g0111961 48.372 215 95 4 1 203 255 465 2.08e-62 202
Msa1164370 MtrunA17_Chr2g0308501 47.222 216 96 5 7 205 99 313 2.26e-61 195
Msa1164370 MtrunA17_Chr4g0057131 45.370 216 102 4 1 204 244 455 1.44e-56 186
Msa1164370 MtrunA17_Chr1g0176241 28.502 207 130 9 1 198 284 481 3.15e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1164370 AT4G33270.1 74.274 241 47 3 1 226 217 457 1.48e-123 358
Msa1164370 AT4G33260.1 74.274 241 47 3 1 226 207 447 1.66e-118 345
Msa1164370 AT4G33260.2 72.321 224 47 3 1 209 207 430 3.76e-108 318
Msa1164370 AT5G27570.1 67.901 243 61 5 1 226 208 450 4.60e-107 316
Msa1164370 AT5G27945.1 66.942 242 64 4 1 226 187 428 4.74e-106 312
Msa1164370 AT5G27080.1 65.432 243 67 5 1 226 200 442 1.69e-102 304
Msa1164370 AT5G26900.1 65.289 242 68 4 1 226 203 444 9.45e-102 302
Msa1164370 AT5G13840.1 48.372 215 95 4 1 203 250 460 7.16e-63 203
Msa1164370 AT5G13840.2 48.372 215 95 4 1 203 250 460 7.16e-63 203
Msa1164370 AT4G22910.1 45.581 215 101 4 2 204 253 463 1.06e-58 192
Msa1164370 AT4G11920.1 45.370 216 102 4 1 204 244 455 2.20e-57 189

Find 69 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATGAAACTCTTAGATTT+TGG 0.143277 7_4:-56016946 Msa1164370:CDS
GTGGCTTTGCATGCTTCTGC+TGG 0.230906 7_4:+56016908 None:intergenic
CCTTTGGAAGTATCCTTCTA+TGG 0.254046 7_4:-56017056 Msa1164370:CDS
CAGGGTAATCTACTGGCTTC+TGG 0.309255 7_4:-56017235 Msa1164370:CDS
GTATCCTTCTATGGTGAAAA+TGG 0.311467 7_4:-56017047 Msa1164370:CDS
AAGGGCTCATTATATGTCTT+TGG 0.358151 7_4:+56016884 None:intergenic
ACGATAATGTTGTTCACATA+TGG 0.371186 7_4:-56017352 Msa1164370:CDS
AATCTACTGGCTTCTGGTGG+AGG 0.376592 7_4:-56017229 Msa1164370:CDS
GTCATCATCTTCAACGAATA+CGG 0.384451 7_4:+56016849 None:intergenic
TTAGTAGCTCACGCTCATTC+TGG 0.385492 7_4:+56017110 None:intergenic
CACTTCGAGGGTGATGTATA+TGG 0.388915 7_4:-56017008 Msa1164370:CDS
GCACTGGCTTGGTGTCCCTC+AGG 0.408143 7_4:-56017254 Msa1164370:CDS
TGGCTTTGCATGCTTCTGCT+GGG 0.422084 7_4:+56016909 None:intergenic
CCGAGAACCAGCTTACCCTT+TGG 0.427049 7_4:-56017072 Msa1164370:CDS
CTCACATCGTTGAAACATAC+AGG 0.428220 7_4:-56017442 Msa1164370:CDS
CTACTGGCTTCTGGTGGAGG+TGG 0.431389 7_4:-56017226 Msa1164370:CDS
AACAATCATATTCTGACAAC+AGG 0.439142 7_4:-56017509 Msa1164370:CDS
CTGGCTTCTGGTGGAGGTGG+AGG 0.440261 7_4:-56017223 Msa1164370:CDS
CAAGAGTGGGTTCACTGTCT+TGG 0.449748 7_4:-56017532 Msa1164370:CDS
CGCAAGTATGTGCTCTGCTA+TGG 0.473307 7_4:-56017135 Msa1164370:CDS
CTCAAACCTGTGAAGACATC+GGG 0.475694 7_4:+56017298 None:intergenic
GATGCCACTGTACACCCATC+TGG 0.480407 7_4:+56016977 None:intergenic
ATCTCAACGGTCACACTTCG+AGG 0.480528 7_4:-56017021 Msa1164370:CDS
CCAAAGGGTAAGCTGGTTCT+CGG 0.481991 7_4:+56017072 None:intergenic
ACTGCAGCTAAGGGCAATGA+AGG 0.483352 7_4:-56017567 None:intergenic
CGATAATGTTGTTCACATAT+GGG 0.487900 7_4:-56017351 Msa1164370:CDS
TGTCCCTCAGGGTAATCTAC+TGG 0.491265 7_4:-56017242 Msa1164370:CDS
GATACTTCCAAAGGGTAAGC+TGG 0.503616 7_4:+56017065 None:intergenic
CACCTACCCGATGTCTTCAC+AGG 0.510822 7_4:-56017304 Msa1164370:intron
AACATACAGGGGACACAACC+AGG 0.511067 7_4:-56017429 Msa1164370:CDS
CTGCTGTGAAGGCACTGGCT+TGG 0.516266 7_4:-56017265 Msa1164370:intron
CTCATTATATGTCTTTGGTG+TGG 0.516280 7_4:+56016889 None:intergenic
TATTCTGACAACAGGAGGAA+TGG 0.524698 7_4:-56017501 Msa1164370:CDS
GGGCTCAAGTGGTCACCCTC+AGG 0.528586 7_4:-56017398 Msa1164370:intron
TCATCATCTTCAACGAATAC+GGG 0.547121 7_4:+56016850 None:intergenic
GGGTGGACACAGAGCAAGAG+TGG 0.549117 7_4:-56017546 Msa1164370:CDS
GGTAATCTACTGGCTTCTGG+TGG 0.565300 7_4:-56017232 Msa1164370:CDS
TATATGGCGCAAAGTCCAGA+TGG 0.565373 7_4:-56016992 Msa1164370:CDS
CGTGAGCTACTAAGCTCACA+TGG 0.568122 7_4:-56017100 Msa1164370:CDS
CTGACAACAGGAGGAATGGA+TGG 0.572412 7_4:-56017497 Msa1164370:CDS
CACTGGCTTGGTGTCCCTCA+GGG 0.575364 7_4:-56017253 Msa1164370:CDS
CATAGAAGGATACTTCCAAA+GGG 0.584766 7_4:+56017057 None:intergenic
CCTCAAACCTGTGAAGACAT+CGG 0.590741 7_4:+56017297 None:intergenic
TCACATATGGGATAGATCTG+TGG 0.596581 7_4:-56017339 Msa1164370:CDS
AGATTGAATTCAGTTGACAC+TGG 0.597762 7_4:-56017160 Msa1164370:CDS
GGACAACAATTGTCAAGTGG+TGG 0.599505 7_4:-56017377 Msa1164370:CDS
ATACGGGTGAAATTAGCAAA+GGG 0.600657 7_4:+56016866 None:intergenic
TCTCAACGGTCACACTTCGA+GGG 0.613321 7_4:-56017020 Msa1164370:CDS
GTGAAAATGGCAGATCTCAA+CGG 0.613838 7_4:-56017034 Msa1164370:CDS
AATACGGGTGAAATTAGCAA+AGG 0.619999 7_4:+56016865 None:intergenic
CAGCTAAGGGCAATGAAGGG+TGG 0.622963 7_4:-56017563 Msa1164370:CDS
CCATAGAAGGATACTTCCAA+AGG 0.623454 7_4:+56017056 None:intergenic
AAGCTGTGGAACACACACAC+TGG 0.630912 7_4:-56017187 Msa1164370:CDS
GTGATCATTGCATTAAGCTG+TGG 0.635919 7_4:-56017201 Msa1164370:CDS
AATCATATTCTGACAACAGG+AGG 0.641326 7_4:-56017506 Msa1164370:CDS
ACTTGACAATTGTTGTCCTG+AGG 0.654097 7_4:+56017382 None:intergenic
TCACATCGTTGAAACATACA+GGG 0.656486 7_4:-56017441 Msa1164370:CDS
CTTGACAATTGTTGTCCTGA+GGG 0.657303 7_4:+56017383 None:intergenic
AAGCCAGTAGATTACCCTGA+GGG 0.662555 7_4:+56017239 None:intergenic
TCAGGACAACAATTGTCAAG+TGG 0.664718 7_4:-56017380 Msa1164370:CDS
TGGGTGTACAGTGGCATCTG+CGG 0.666837 7_4:-56016972 Msa1164370:CDS
CTGCAGCTAAGGGCAATGAA+GGG 0.668716 7_4:-56017566 None:intergenic
GGTGGACACAGAGCAAGAGT+GGG 0.681424 7_4:-56017545 Msa1164370:CDS
AAGTCCAGATGGGTGTACAG+TGG 0.686450 7_4:-56016981 Msa1164370:CDS
ATATGGCGCAAAGTCCAGAT+GGG 0.692355 7_4:-56016991 Msa1164370:CDS
AACCTGTGAAGACATCGGGT+AGG 0.717836 7_4:+56017302 None:intergenic
CACATCGTTGAAACATACAG+GGG 0.758385 7_4:-56017440 Msa1164370:CDS
GAAGCCAGTAGATTACCCTG+AGG 0.773885 7_4:+56017238 None:intergenic
ATACAGGGGACACAACCAGG+AGG 0.774768 7_4:-56017426 Msa1164370:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGATTTTGGAATGTCTTT+GGG - chr7_4:56017485-56017504 Msa1164370:CDS 25.0%
AACAATCATATTCTGACAAC+AGG - chr7_4:56016910-56016929 Msa1164370:CDS 30.0%
ACGATAATGTTGTTCACATA+TGG - chr7_4:56017067-56017086 Msa1164370:CDS 30.0%
CGATAATGTTGTTCACATAT+GGG - chr7_4:56017068-56017087 Msa1164370:CDS 30.0%
! CAGATGAAACTCTTAGATTT+TGG - chr7_4:56017473-56017492 Msa1164370:CDS 30.0%
!!! CTTAGATTTTGGAATGTCTT+TGG - chr7_4:56017484-56017503 Msa1164370:CDS 30.0%
AATCATATTCTGACAACAGG+AGG - chr7_4:56016913-56016932 Msa1164370:CDS 35.0%
TCACATCGTTGAAACATACA+GGG - chr7_4:56016978-56016997 Msa1164370:CDS 35.0%
AGATTGAATTCAGTTGACAC+TGG - chr7_4:56017259-56017278 Msa1164370:intron 35.0%
CATAGAAGGATACTTCCAAA+GGG + chr7_4:56017365-56017384 None:intergenic 35.0%
GTATCCTTCTATGGTGAAAA+TGG - chr7_4:56017372-56017391 Msa1164370:CDS 35.0%
CTCATTATATGTCTTTGGTG+TGG + chr7_4:56017533-56017552 None:intergenic 35.0%
AAGGGCTCATTATATGTCTT+TGG + chr7_4:56017538-56017557 None:intergenic 35.0%
ATACGGGTGAAATTAGCAAA+GGG + chr7_4:56017556-56017575 None:intergenic 35.0%
AATACGGGTGAAATTAGCAA+AGG + chr7_4:56017557-56017576 None:intergenic 35.0%
TATTCTGACAACAGGAGGAA+TGG - chr7_4:56016918-56016937 Msa1164370:CDS 40.0%
CTCACATCGTTGAAACATAC+AGG - chr7_4:56016977-56016996 Msa1164370:CDS 40.0%
CACATCGTTGAAACATACAG+GGG - chr7_4:56016979-56016998 Msa1164370:CDS 40.0%
CTTGACAATTGTTGTCCTGA+GGG + chr7_4:56017039-56017058 None:intergenic 40.0%
ACTTGACAATTGTTGTCCTG+AGG + chr7_4:56017040-56017059 None:intergenic 40.0%
TCAGGACAACAATTGTCAAG+TGG - chr7_4:56017039-56017058 Msa1164370:CDS 40.0%
TCACATATGGGATAGATCTG+TGG - chr7_4:56017080-56017099 Msa1164370:CDS 40.0%
GTGATCATTGCATTAAGCTG+TGG - chr7_4:56017218-56017237 Msa1164370:CDS 40.0%
CCATAGAAGGATACTTCCAA+AGG + chr7_4:56017366-56017385 None:intergenic 40.0%
!! CCTTTGGAAGTATCCTTCTA+TGG - chr7_4:56017363-56017382 Msa1164370:CDS 40.0%
!!! TCTGCCATTTTCACCATAGA+AGG + chr7_4:56017379-56017398 None:intergenic 40.0%
GTGAAAATGGCAGATCTCAA+CGG - chr7_4:56017385-56017404 Msa1164370:CDS 40.0%
GGACAACAATTGTCAAGTGG+TGG - chr7_4:56017042-56017061 Msa1164370:CDS 45.0%
CTCAAACCTGTGAAGACATC+GGG + chr7_4:56017124-56017143 None:intergenic 45.0%
CCTCAAACCTGTGAAGACAT+CGG + chr7_4:56017125-56017144 None:intergenic 45.0%
AAGCCAGTAGATTACCCTGA+GGG + chr7_4:56017183-56017202 None:intergenic 45.0%
TTAGTAGCTCACGCTCATTC+TGG + chr7_4:56017312-56017331 None:intergenic 45.0%
GATACTTCCAAAGGGTAAGC+TGG + chr7_4:56017357-56017376 None:intergenic 45.0%
CACTTCGAGGGTGATGTATA+TGG - chr7_4:56017411-56017430 Msa1164370:intron 45.0%
TATATGGCGCAAAGTCCAGA+TGG - chr7_4:56017427-56017446 Msa1164370:CDS 45.0%
ATATGGCGCAAAGTCCAGAT+GGG - chr7_4:56017428-56017447 Msa1164370:CDS 45.0%
! CAAGAGTGGGTTCACTGTCT+TGG - chr7_4:56016887-56016906 Msa1164370:CDS 50.0%
CTGACAACAGGAGGAATGGA+TGG - chr7_4:56016922-56016941 Msa1164370:CDS 50.0%
AACATACAGGGGACACAACC+AGG - chr7_4:56016990-56017009 Msa1164370:CDS 50.0%
GACACAACCAGGAGGTTTGT+GGG - chr7_4:56017001-56017020 Msa1164370:CDS 50.0%
AACCTGTGAAGACATCGGGT+AGG + chr7_4:56017120-56017139 None:intergenic 50.0%
CCGATGTCTTCACAGGTTTG+AGG - chr7_4:56017122-56017141 Msa1164370:CDS 50.0%
GGAACACATAGCTGCTGTGA+AGG - chr7_4:56017143-56017162 Msa1164370:CDS 50.0%
TGTCCCTCAGGGTAATCTAC+TGG - chr7_4:56017177-56017196 Msa1164370:CDS 50.0%
GAAGCCAGTAGATTACCCTG+AGG + chr7_4:56017184-56017203 None:intergenic 50.0%
!! CAGGGTAATCTACTGGCTTC+TGG - chr7_4:56017184-56017203 Msa1164370:CDS 50.0%
!! GGTAATCTACTGGCTTCTGG+TGG - chr7_4:56017187-56017206 Msa1164370:CDS 50.0%
!! AATCTACTGGCTTCTGGTGG+AGG - chr7_4:56017190-56017209 Msa1164370:CDS 50.0%
AAGCTGTGGAACACACACAC+TGG - chr7_4:56017232-56017251 Msa1164370:CDS 50.0%
CGCAAGTATGTGCTCTGCTA+TGG - chr7_4:56017284-56017303 Msa1164370:intron 50.0%
CGTGAGCTACTAAGCTCACA+TGG - chr7_4:56017319-56017338 Msa1164370:CDS 50.0%
! CCAAAGGGTAAGCTGGTTCT+CGG + chr7_4:56017350-56017369 None:intergenic 50.0%
ATCTCAACGGTCACACTTCG+AGG - chr7_4:56017398-56017417 Msa1164370:intron 50.0%
TCTCAACGGTCACACTTCGA+GGG - chr7_4:56017399-56017418 Msa1164370:intron 50.0%
AAGTCCAGATGGGTGTACAG+TGG - chr7_4:56017438-56017457 Msa1164370:CDS 50.0%
! TGGCTTTGCATGCTTCTGCT+GGG + chr7_4:56017513-56017532 None:intergenic 50.0%
GGTGGACACAGAGCAAGAGT+GGG - chr7_4:56016874-56016893 Msa1164370:CDS 55.0%
ATACAGGGGACACAACCAGG+AGG - chr7_4:56016993-56017012 Msa1164370:CDS 55.0%
GGACACAACCAGGAGGTTTG+TGG - chr7_4:56017000-56017019 Msa1164370:CDS 55.0%
ACTTGAGCCCACAAACCTCC+TGG + chr7_4:56017011-56017030 None:intergenic 55.0%
AGGAGGTTTGTGGGCTCAAG+TGG - chr7_4:56017010-56017029 Msa1164370:CDS 55.0%
CACCTACCCGATGTCTTCAC+AGG - chr7_4:56017115-56017134 Msa1164370:CDS 55.0%
! CATAGCTGCTGTGAAGGCAC+TGG - chr7_4:56017149-56017168 Msa1164370:CDS 55.0%
CCGAGAACCAGCTTACCCTT+TGG - chr7_4:56017347-56017366 Msa1164370:CDS 55.0%
GATGCCACTGTACACCCATC+TGG + chr7_4:56017445-56017464 None:intergenic 55.0%
TGGGTGTACAGTGGCATCTG+CGG - chr7_4:56017447-56017466 Msa1164370:CDS 55.0%
! GTGGCTTTGCATGCTTCTGC+TGG + chr7_4:56017514-56017533 None:intergenic 55.0%
GGGTGGACACAGAGCAAGAG+TGG - chr7_4:56016873-56016892 Msa1164370:CDS 60.0%
!! CTGCTGTGAAGGCACTGGCT+TGG - chr7_4:56017154-56017173 Msa1164370:CDS 60.0%
!! CACTGGCTTGGTGTCCCTCA+GGG - chr7_4:56017166-56017185 Msa1164370:CDS 60.0%
!! CTACTGGCTTCTGGTGGAGG+TGG - chr7_4:56017193-56017212 Msa1164370:CDS 60.0%
GGGCTCAAGTGGTCACCCTC+AGG - chr7_4:56017021-56017040 Msa1164370:CDS 65.0%
!! GCACTGGCTTGGTGTCCCTC+AGG - chr7_4:56017165-56017184 Msa1164370:CDS 65.0%
CTGGCTTCTGGTGGAGGTGG+AGG - chr7_4:56017196-56017215 Msa1164370:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 56016859 56017582 56016859 ID=Msa1164370;Name=Msa1164370
chr7_4 mRNA 56016859 56017582 56016859 ID=Msa1164370-mRNA-1;Parent=Msa1164370;Name=Msa1164370-mRNA-1;_AED=0.03;_eAED=0.06;_QI=0|0|0|1|0|0.33|3|0|226
chr7_4 exon 56017427 56017582 56017427 ID=Msa1164370-mRNA-1:exon:16594;Parent=Msa1164370-mRNA-1
chr7_4 exon 56017305 56017411 56017305 ID=Msa1164370-mRNA-1:exon:16593;Parent=Msa1164370-mRNA-1
chr7_4 exon 56016859 56017276 56016859 ID=Msa1164370-mRNA-1:exon:16592;Parent=Msa1164370-mRNA-1
chr7_4 CDS 56017427 56017582 56017427 ID=Msa1164370-mRNA-1:cds;Parent=Msa1164370-mRNA-1
chr7_4 CDS 56017305 56017411 56017305 ID=Msa1164370-mRNA-1:cds;Parent=Msa1164370-mRNA-1
chr7_4 CDS 56016859 56017276 56016859 ID=Msa1164370-mRNA-1:cds;Parent=Msa1164370-mRNA-1
Gene Sequence

>Msa1164370

CTAAGGGCAATGAAGGGTGGACACAGAGCAAGAGTGGGTTCACTGTCTTGGAACAATCATATTCTGACAACAGGAGGAATGGATGGTAAAGTTGTAAACAATGATGTTAGAGTGAGATCTCACATCGTTGAAACATACAGGGGACACAACCAGGAGTGGTCACCCTCAGGACAACAATTGTCAAGTGGTGGAAACGATAATGTTGTTCACATATGGGATAGATCTGTGGTTTCTTCAAATTCACCTACCCGATGTCTTCACAGGCACTGGCTTGGTGTCCCTCAGGGTAATCTACTGGCTTCTGGTGGAGGTGGAGGTGATCATTGCATTAAGCTGTGGAACACACACACTGGTGCGAGATTGAATTCAGTTGACACTGGATCGCAAGTATGTGCTCTGCTATGGAACCAGAATGAGCGTGAGCTACTAAGCTCACATGGCTTCACCGAGAACCAGCTTACCCTTTGGAAGTATCCTTCTATGGTGAAAATGGCAGATCTCAACGGTCACACTTCGAGGGTGATGTATATGGCGCAAAGTCCAGATGGGTGTACAGTGGCATCTGCGGCTGCAGATGAAACTCTTAGATTTTGGAATGTCTTTGGGAGCCCAGCAGAAGCATGCAAAGCCACACCAAAGACATATAATGAGCCCTTTGCTAATTTCACCCGTATTCGTTGA

Protein sequence

>Msa1164370

LRAMKGGHRARVGSLSWNNHILTTGGMDGKVVNNDVRVRSHIVETYRGHNQEWSPSGQQLSSGGNDNVVHIWDRSVVSSNSPTRCLHRHWLGVPQGNLLASGGGGGDHCIKLWNTHTGARLNSVDTGSQVCALLWNQNERELLSSHGFTENQLTLWKYPSMVKMADLNGHTSRVMYMAQSPDGCTVASAAADETLRFWNVFGSPAEACKATPKTYNEPFANFTRIR*