AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1123120


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1123120 MtrunA17_Chr7g0267961 89.256 242 10 4 1 226 216 457 6.50e-151 427
Msa1123120 MtrunA17_Chr5g0395821 72.199 241 52 3 1 226 214 454 5.86e-124 358
Msa1123120 MtrunA17_Chr3g0098921 70.248 242 56 4 1 226 218 459 5.71e-119 346
Msa1123120 MtrunA17_Chr7g0228191 76.211 227 37 4 1 210 103 329 1.63e-116 335
Msa1123120 MtrunA17_Chr7g0235181 71.193 243 48 7 1 226 189 426 8.92e-113 329
Msa1123120 MtrunA17_Chr7g0233651 67.220 241 55 4 1 226 200 431 3.83e-107 315
Msa1123120 MtrunA17_Chr7g0234591 68.724 243 56 5 1 226 197 436 5.92e-107 315
Msa1123120 MtrunA17_Chr2g0330601 62.771 231 46 6 7 224 166 369 2.50e-90 270
Msa1123120 MtrunA17_Chr2g0330681 65.241 187 39 6 7 180 196 369 4.51e-70 218
Msa1123120 MtrunA17_Chr3g0111961 48.372 215 95 4 1 203 255 465 2.08e-62 202
Msa1123120 MtrunA17_Chr2g0308501 47.222 216 96 5 7 205 99 313 2.26e-61 195
Msa1123120 MtrunA17_Chr4g0057131 45.370 216 102 4 1 204 244 455 1.44e-56 186
Msa1123120 MtrunA17_Chr1g0176241 28.502 207 130 9 1 198 284 481 3.15e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1123120 AT4G33270.1 74.274 241 47 3 1 226 217 457 1.48e-123 358
Msa1123120 AT4G33260.1 74.274 241 47 3 1 226 207 447 1.66e-118 345
Msa1123120 AT4G33260.2 72.321 224 47 3 1 209 207 430 3.76e-108 318
Msa1123120 AT5G27570.1 67.901 243 61 5 1 226 208 450 4.60e-107 316
Msa1123120 AT5G27945.1 66.942 242 64 4 1 226 187 428 4.74e-106 312
Msa1123120 AT5G27080.1 65.432 243 67 5 1 226 200 442 1.69e-102 304
Msa1123120 AT5G26900.1 65.289 242 68 4 1 226 203 444 9.45e-102 302
Msa1123120 AT5G13840.1 48.372 215 95 4 1 203 250 460 7.16e-63 203
Msa1123120 AT5G13840.2 48.372 215 95 4 1 203 250 460 7.16e-63 203
Msa1123120 AT4G22910.1 45.581 215 101 4 2 204 253 463 1.06e-58 192
Msa1123120 AT4G11920.1 45.370 216 102 4 1 204 244 455 2.20e-57 189

Find 69 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATGAAACTCTTAGATTT+TGG 0.143277 7_3:-73867597 Msa1123120:CDS
GTGGCTTTGCATGCTTCTGC+TGG 0.230906 7_3:+73867559 None:intergenic
CCTTTGGAAGTATCCTTCTA+TGG 0.254046 7_3:-73867707 Msa1123120:CDS
CAGGGTAATCTACTGGCTTC+TGG 0.309255 7_3:-73867886 Msa1123120:CDS
GTATCCTTCTATGGTGAAAA+TGG 0.311467 7_3:-73867698 Msa1123120:CDS
AAGGGCTCATTATATGTCTT+TGG 0.358151 7_3:+73867535 None:intergenic
ACGATAATGTTGTTCACATA+TGG 0.371186 7_3:-73868003 Msa1123120:CDS
AATCTACTGGCTTCTGGTGG+AGG 0.376592 7_3:-73867880 Msa1123120:CDS
GTCATCATCTTCAACGAATA+CGG 0.384451 7_3:+73867500 None:intergenic
TTAGTAGCTCACGCTCATTC+TGG 0.385492 7_3:+73867761 None:intergenic
CACTTCGAGGGTGATGTATA+TGG 0.388915 7_3:-73867659 Msa1123120:CDS
GCACTGGCTTGGTGTCCCTC+AGG 0.408143 7_3:-73867905 Msa1123120:CDS
TGGCTTTGCATGCTTCTGCT+GGG 0.422084 7_3:+73867560 None:intergenic
CCGAGAACCAGCTTACCCTT+TGG 0.427049 7_3:-73867723 Msa1123120:CDS
CTCACATCGTTGAAACATAC+AGG 0.428220 7_3:-73868093 Msa1123120:CDS
CTACTGGCTTCTGGTGGAGG+TGG 0.431389 7_3:-73867877 Msa1123120:CDS
AACAATCATATTCTGACAAC+AGG 0.439142 7_3:-73868160 Msa1123120:CDS
CTGGCTTCTGGTGGAGGTGG+AGG 0.440261 7_3:-73867874 Msa1123120:CDS
CAAGAGTGGGTTCACTGTCT+TGG 0.449748 7_3:-73868183 Msa1123120:CDS
CGCAAGTATGTGCTCTGCTA+TGG 0.473307 7_3:-73867786 Msa1123120:CDS
CTCAAACCTGTGAAGACATC+GGG 0.475694 7_3:+73867949 None:intergenic
GATGCCACTGTACACCCATC+TGG 0.480407 7_3:+73867628 None:intergenic
ATCTCAACGGTCACACTTCG+AGG 0.480528 7_3:-73867672 Msa1123120:CDS
CCAAAGGGTAAGCTGGTTCT+CGG 0.481991 7_3:+73867723 None:intergenic
ACTGCAGCTAAGGGCAATGA+AGG 0.483352 7_3:-73868218 None:intergenic
CGATAATGTTGTTCACATAT+GGG 0.487900 7_3:-73868002 Msa1123120:CDS
TGTCCCTCAGGGTAATCTAC+TGG 0.491265 7_3:-73867893 Msa1123120:CDS
GATACTTCCAAAGGGTAAGC+TGG 0.503616 7_3:+73867716 None:intergenic
CACCTACCCGATGTCTTCAC+AGG 0.510822 7_3:-73867955 Msa1123120:intron
AACATACAGGGGACACAACC+AGG 0.511067 7_3:-73868080 Msa1123120:CDS
CTGCTGTGAAGGCACTGGCT+TGG 0.516266 7_3:-73867916 Msa1123120:intron
CTCATTATATGTCTTTGGTG+TGG 0.516280 7_3:+73867540 None:intergenic
TATTCTGACAACAGGAGGAA+TGG 0.524698 7_3:-73868152 Msa1123120:CDS
GGGCTCAAGTGGTCACCCTC+AGG 0.528586 7_3:-73868049 Msa1123120:intron
TCATCATCTTCAACGAATAC+GGG 0.547121 7_3:+73867501 None:intergenic
GGGTGGACACAGAGCAAGAG+TGG 0.549117 7_3:-73868197 Msa1123120:CDS
GGTAATCTACTGGCTTCTGG+TGG 0.565300 7_3:-73867883 Msa1123120:CDS
TATATGGCGCAAAGTCCAGA+TGG 0.565373 7_3:-73867643 Msa1123120:CDS
CGTGAGCTACTAAGCTCACA+TGG 0.568122 7_3:-73867751 Msa1123120:CDS
CTGACAACAGGAGGAATGGA+TGG 0.572412 7_3:-73868148 Msa1123120:CDS
CACTGGCTTGGTGTCCCTCA+GGG 0.575364 7_3:-73867904 Msa1123120:CDS
CATAGAAGGATACTTCCAAA+GGG 0.584766 7_3:+73867708 None:intergenic
CCTCAAACCTGTGAAGACAT+CGG 0.590741 7_3:+73867948 None:intergenic
TCACATATGGGATAGATCTG+TGG 0.596581 7_3:-73867990 Msa1123120:CDS
AGATTGAATTCAGTTGACAC+TGG 0.597762 7_3:-73867811 Msa1123120:CDS
GGACAACAATTGTCAAGTGG+TGG 0.599505 7_3:-73868028 Msa1123120:CDS
ATACGGGTGAAATTAGCAAA+GGG 0.600657 7_3:+73867517 None:intergenic
TCTCAACGGTCACACTTCGA+GGG 0.613321 7_3:-73867671 Msa1123120:CDS
GTGAAAATGGCAGATCTCAA+CGG 0.613838 7_3:-73867685 Msa1123120:CDS
AATACGGGTGAAATTAGCAA+AGG 0.619999 7_3:+73867516 None:intergenic
CAGCTAAGGGCAATGAAGGG+TGG 0.622963 7_3:-73868214 Msa1123120:CDS
CCATAGAAGGATACTTCCAA+AGG 0.623454 7_3:+73867707 None:intergenic
AAGCTGTGGAACACACACAC+TGG 0.630912 7_3:-73867838 Msa1123120:CDS
GTGATCATTGCATTAAGCTG+TGG 0.635919 7_3:-73867852 Msa1123120:CDS
AATCATATTCTGACAACAGG+AGG 0.641326 7_3:-73868157 Msa1123120:CDS
ACTTGACAATTGTTGTCCTG+AGG 0.654097 7_3:+73868033 None:intergenic
TCACATCGTTGAAACATACA+GGG 0.656486 7_3:-73868092 Msa1123120:CDS
CTTGACAATTGTTGTCCTGA+GGG 0.657303 7_3:+73868034 None:intergenic
AAGCCAGTAGATTACCCTGA+GGG 0.662555 7_3:+73867890 None:intergenic
TCAGGACAACAATTGTCAAG+TGG 0.664718 7_3:-73868031 Msa1123120:CDS
TGGGTGTACAGTGGCATCTG+CGG 0.666837 7_3:-73867623 Msa1123120:CDS
CTGCAGCTAAGGGCAATGAA+GGG 0.668716 7_3:-73868217 None:intergenic
GGTGGACACAGAGCAAGAGT+GGG 0.681424 7_3:-73868196 Msa1123120:CDS
AAGTCCAGATGGGTGTACAG+TGG 0.686450 7_3:-73867632 Msa1123120:CDS
ATATGGCGCAAAGTCCAGAT+GGG 0.692355 7_3:-73867642 Msa1123120:CDS
AACCTGTGAAGACATCGGGT+AGG 0.717836 7_3:+73867953 None:intergenic
CACATCGTTGAAACATACAG+GGG 0.758385 7_3:-73868091 Msa1123120:CDS
GAAGCCAGTAGATTACCCTG+AGG 0.773885 7_3:+73867889 None:intergenic
ATACAGGGGACACAACCAGG+AGG 0.774768 7_3:-73868077 Msa1123120:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGATTTTGGAATGTCTTT+GGG - chr7_3:73868136-73868155 Msa1123120:CDS 25.0%
AACAATCATATTCTGACAAC+AGG - chr7_3:73867561-73867580 Msa1123120:CDS 30.0%
ACGATAATGTTGTTCACATA+TGG - chr7_3:73867718-73867737 Msa1123120:CDS 30.0%
CGATAATGTTGTTCACATAT+GGG - chr7_3:73867719-73867738 Msa1123120:CDS 30.0%
! CAGATGAAACTCTTAGATTT+TGG - chr7_3:73868124-73868143 Msa1123120:CDS 30.0%
!!! CTTAGATTTTGGAATGTCTT+TGG - chr7_3:73868135-73868154 Msa1123120:CDS 30.0%
AATCATATTCTGACAACAGG+AGG - chr7_3:73867564-73867583 Msa1123120:CDS 35.0%
TCACATCGTTGAAACATACA+GGG - chr7_3:73867629-73867648 Msa1123120:CDS 35.0%
AGATTGAATTCAGTTGACAC+TGG - chr7_3:73867910-73867929 Msa1123120:intron 35.0%
CATAGAAGGATACTTCCAAA+GGG + chr7_3:73868016-73868035 None:intergenic 35.0%
GTATCCTTCTATGGTGAAAA+TGG - chr7_3:73868023-73868042 Msa1123120:CDS 35.0%
CTCATTATATGTCTTTGGTG+TGG + chr7_3:73868184-73868203 None:intergenic 35.0%
AAGGGCTCATTATATGTCTT+TGG + chr7_3:73868189-73868208 None:intergenic 35.0%
ATACGGGTGAAATTAGCAAA+GGG + chr7_3:73868207-73868226 None:intergenic 35.0%
AATACGGGTGAAATTAGCAA+AGG + chr7_3:73868208-73868227 None:intergenic 35.0%
TATTCTGACAACAGGAGGAA+TGG - chr7_3:73867569-73867588 Msa1123120:CDS 40.0%
CTCACATCGTTGAAACATAC+AGG - chr7_3:73867628-73867647 Msa1123120:CDS 40.0%
CACATCGTTGAAACATACAG+GGG - chr7_3:73867630-73867649 Msa1123120:CDS 40.0%
CTTGACAATTGTTGTCCTGA+GGG + chr7_3:73867690-73867709 None:intergenic 40.0%
ACTTGACAATTGTTGTCCTG+AGG + chr7_3:73867691-73867710 None:intergenic 40.0%
TCAGGACAACAATTGTCAAG+TGG - chr7_3:73867690-73867709 Msa1123120:CDS 40.0%
TCACATATGGGATAGATCTG+TGG - chr7_3:73867731-73867750 Msa1123120:CDS 40.0%
GTGATCATTGCATTAAGCTG+TGG - chr7_3:73867869-73867888 Msa1123120:CDS 40.0%
CCATAGAAGGATACTTCCAA+AGG + chr7_3:73868017-73868036 None:intergenic 40.0%
!! CCTTTGGAAGTATCCTTCTA+TGG - chr7_3:73868014-73868033 Msa1123120:CDS 40.0%
!!! TCTGCCATTTTCACCATAGA+AGG + chr7_3:73868030-73868049 None:intergenic 40.0%
GTGAAAATGGCAGATCTCAA+CGG - chr7_3:73868036-73868055 Msa1123120:CDS 40.0%
GGACAACAATTGTCAAGTGG+TGG - chr7_3:73867693-73867712 Msa1123120:CDS 45.0%
CTCAAACCTGTGAAGACATC+GGG + chr7_3:73867775-73867794 None:intergenic 45.0%
CCTCAAACCTGTGAAGACAT+CGG + chr7_3:73867776-73867795 None:intergenic 45.0%
AAGCCAGTAGATTACCCTGA+GGG + chr7_3:73867834-73867853 None:intergenic 45.0%
TTAGTAGCTCACGCTCATTC+TGG + chr7_3:73867963-73867982 None:intergenic 45.0%
GATACTTCCAAAGGGTAAGC+TGG + chr7_3:73868008-73868027 None:intergenic 45.0%
CACTTCGAGGGTGATGTATA+TGG - chr7_3:73868062-73868081 Msa1123120:intron 45.0%
TATATGGCGCAAAGTCCAGA+TGG - chr7_3:73868078-73868097 Msa1123120:CDS 45.0%
ATATGGCGCAAAGTCCAGAT+GGG - chr7_3:73868079-73868098 Msa1123120:CDS 45.0%
! CAAGAGTGGGTTCACTGTCT+TGG - chr7_3:73867538-73867557 Msa1123120:CDS 50.0%
CTGACAACAGGAGGAATGGA+TGG - chr7_3:73867573-73867592 Msa1123120:CDS 50.0%
AACATACAGGGGACACAACC+AGG - chr7_3:73867641-73867660 Msa1123120:CDS 50.0%
GACACAACCAGGAGGTTTGT+GGG - chr7_3:73867652-73867671 Msa1123120:CDS 50.0%
AACCTGTGAAGACATCGGGT+AGG + chr7_3:73867771-73867790 None:intergenic 50.0%
CCGATGTCTTCACAGGTTTG+AGG - chr7_3:73867773-73867792 Msa1123120:CDS 50.0%
GGAACACATAGCTGCTGTGA+AGG - chr7_3:73867794-73867813 Msa1123120:CDS 50.0%
TGTCCCTCAGGGTAATCTAC+TGG - chr7_3:73867828-73867847 Msa1123120:CDS 50.0%
GAAGCCAGTAGATTACCCTG+AGG + chr7_3:73867835-73867854 None:intergenic 50.0%
!! CAGGGTAATCTACTGGCTTC+TGG - chr7_3:73867835-73867854 Msa1123120:CDS 50.0%
!! GGTAATCTACTGGCTTCTGG+TGG - chr7_3:73867838-73867857 Msa1123120:CDS 50.0%
!! AATCTACTGGCTTCTGGTGG+AGG - chr7_3:73867841-73867860 Msa1123120:CDS 50.0%
AAGCTGTGGAACACACACAC+TGG - chr7_3:73867883-73867902 Msa1123120:CDS 50.0%
CGCAAGTATGTGCTCTGCTA+TGG - chr7_3:73867935-73867954 Msa1123120:intron 50.0%
CGTGAGCTACTAAGCTCACA+TGG - chr7_3:73867970-73867989 Msa1123120:CDS 50.0%
! CCAAAGGGTAAGCTGGTTCT+CGG + chr7_3:73868001-73868020 None:intergenic 50.0%
ATCTCAACGGTCACACTTCG+AGG - chr7_3:73868049-73868068 Msa1123120:intron 50.0%
TCTCAACGGTCACACTTCGA+GGG - chr7_3:73868050-73868069 Msa1123120:intron 50.0%
AAGTCCAGATGGGTGTACAG+TGG - chr7_3:73868089-73868108 Msa1123120:CDS 50.0%
! TGGCTTTGCATGCTTCTGCT+GGG + chr7_3:73868164-73868183 None:intergenic 50.0%
GGTGGACACAGAGCAAGAGT+GGG - chr7_3:73867525-73867544 Msa1123120:CDS 55.0%
ATACAGGGGACACAACCAGG+AGG - chr7_3:73867644-73867663 Msa1123120:CDS 55.0%
GGACACAACCAGGAGGTTTG+TGG - chr7_3:73867651-73867670 Msa1123120:CDS 55.0%
ACTTGAGCCCACAAACCTCC+TGG + chr7_3:73867662-73867681 None:intergenic 55.0%
AGGAGGTTTGTGGGCTCAAG+TGG - chr7_3:73867661-73867680 Msa1123120:CDS 55.0%
CACCTACCCGATGTCTTCAC+AGG - chr7_3:73867766-73867785 Msa1123120:CDS 55.0%
! CATAGCTGCTGTGAAGGCAC+TGG - chr7_3:73867800-73867819 Msa1123120:CDS 55.0%
CCGAGAACCAGCTTACCCTT+TGG - chr7_3:73867998-73868017 Msa1123120:CDS 55.0%
GATGCCACTGTACACCCATC+TGG + chr7_3:73868096-73868115 None:intergenic 55.0%
TGGGTGTACAGTGGCATCTG+CGG - chr7_3:73868098-73868117 Msa1123120:CDS 55.0%
! GTGGCTTTGCATGCTTCTGC+TGG + chr7_3:73868165-73868184 None:intergenic 55.0%
GGGTGGACACAGAGCAAGAG+TGG - chr7_3:73867524-73867543 Msa1123120:CDS 60.0%
!! CTGCTGTGAAGGCACTGGCT+TGG - chr7_3:73867805-73867824 Msa1123120:CDS 60.0%
!! CACTGGCTTGGTGTCCCTCA+GGG - chr7_3:73867817-73867836 Msa1123120:CDS 60.0%
!! CTACTGGCTTCTGGTGGAGG+TGG - chr7_3:73867844-73867863 Msa1123120:CDS 60.0%
GGGCTCAAGTGGTCACCCTC+AGG - chr7_3:73867672-73867691 Msa1123120:CDS 65.0%
!! GCACTGGCTTGGTGTCCCTC+AGG - chr7_3:73867816-73867835 Msa1123120:CDS 65.0%
CTGGCTTCTGGTGGAGGTGG+AGG - chr7_3:73867847-73867866 Msa1123120:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 73867510 73868233 73867510 ID=Msa1123120;Name=Msa1123120
chr7_3 mRNA 73867510 73868233 73867510 ID=Msa1123120-mRNA-1;Parent=Msa1123120;Name=Msa1123120-mRNA-1;_AED=0.03;_eAED=0.06;_QI=0|0|0|1|0|0.33|3|0|226
chr7_3 exon 73868078 73868233 73868078 ID=Msa1123120-mRNA-1:exon:19473;Parent=Msa1123120-mRNA-1
chr7_3 exon 73867956 73868062 73867956 ID=Msa1123120-mRNA-1:exon:19472;Parent=Msa1123120-mRNA-1
chr7_3 exon 73867510 73867927 73867510 ID=Msa1123120-mRNA-1:exon:19471;Parent=Msa1123120-mRNA-1
chr7_3 CDS 73868078 73868233 73868078 ID=Msa1123120-mRNA-1:cds;Parent=Msa1123120-mRNA-1
chr7_3 CDS 73867956 73868062 73867956 ID=Msa1123120-mRNA-1:cds;Parent=Msa1123120-mRNA-1
chr7_3 CDS 73867510 73867927 73867510 ID=Msa1123120-mRNA-1:cds;Parent=Msa1123120-mRNA-1
Gene Sequence

>Msa1123120

CTAAGGGCAATGAAGGGTGGACACAGAGCAAGAGTGGGTTCACTGTCTTGGAACAATCATATTCTGACAACAGGAGGAATGGATGGTAAAGTTGTAAACAATGATGTTAGAGTGAGATCTCACATCGTTGAAACATACAGGGGACACAACCAGGAGTGGTCACCCTCAGGACAACAATTGTCAAGTGGTGGAAACGATAATGTTGTTCACATATGGGATAGATCTGTGGTTTCTTCAAATTCACCTACCCGATGTCTTCACAGGCACTGGCTTGGTGTCCCTCAGGGTAATCTACTGGCTTCTGGTGGAGGTGGAGGTGATCATTGCATTAAGCTGTGGAACACACACACTGGTGCGAGATTGAATTCAGTTGACACTGGATCGCAAGTATGTGCTCTGCTATGGAACCAGAATGAGCGTGAGCTACTAAGCTCACATGGCTTCACCGAGAACCAGCTTACCCTTTGGAAGTATCCTTCTATGGTGAAAATGGCAGATCTCAACGGTCACACTTCGAGGGTGATGTATATGGCGCAAAGTCCAGATGGGTGTACAGTGGCATCTGCGGCTGCAGATGAAACTCTTAGATTTTGGAATGTCTTTGGGAGCCCAGCAGAAGCATGCAAAGCCACACCAAAGACATATAATGAGCCCTTTGCTAATTTCACCCGTATTCGTTGA

Protein sequence

>Msa1123120

LRAMKGGHRARVGSLSWNNHILTTGGMDGKVVNNDVRVRSHIVETYRGHNQEWSPSGQQLSSGGNDNVVHIWDRSVVSSNSPTRCLHRHWLGVPQGNLLASGGGGGDHCIKLWNTHTGARLNSVDTGSQVCALLWNQNERELLSSHGFTENQLTLWKYPSMVKMADLNGHTSRVMYMAQSPDGCTVASAAADETLRFWNVFGSPAEACKATPKTYNEPFANFTRIR*