AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1421790


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1421790 MtrunA17_Chr7g0267961 85.356 239 5 2 1 209 219 457 2.26e-145 412
Msa1421790 MtrunA17_Chr5g0395821 70.168 238 42 1 1 209 217 454 7.64e-122 352
Msa1421790 MtrunA17_Chr3g0098921 68.201 239 46 2 1 209 221 459 5.25e-117 340
Msa1421790 MtrunA17_Chr7g0228191 73.214 224 29 2 1 193 106 329 2.72e-114 329
Msa1421790 MtrunA17_Chr7g0235181 69.167 240 38 4 1 209 192 426 7.05e-113 328
Msa1421790 MtrunA17_Chr7g0233651 64.286 238 47 2 1 209 203 431 5.44e-103 304
Msa1421790 MtrunA17_Chr7g0234591 65.417 240 49 3 1 209 200 436 1.01e-102 303
Msa1421790 MtrunA17_Chr2g0330601 65.385 208 64 3 4 207 166 369 2.38e-88 265
Msa1421790 MtrunA17_Chr3g0111961 48.309 207 84 1 3 186 259 465 3.21e-65 208
Msa1421790 MtrunA17_Chr2g0308501 47.685 216 81 4 4 188 99 313 3.66e-63 199
Msa1421790 MtrunA17_Chr2g0330681 58.115 191 32 4 4 163 196 369 2.00e-62 198
Msa1421790 MtrunA17_Chr4g0057131 46.860 207 87 1 4 187 249 455 1.17e-61 199
Msa1421790 MtrunA17_Chr2g0281451 27.429 175 101 5 3 175 97 247 1.27e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1421790 AT4G33270.1 71.849 238 38 1 1 209 220 457 2.11e-121 352
Msa1421790 AT4G33260.1 71.849 238 38 1 1 209 210 447 2.12e-116 338
Msa1421790 AT5G27570.1 67.083 240 48 3 1 209 211 450 7.72e-107 315
Msa1421790 AT5G27945.1 65.690 239 52 2 1 209 190 428 9.48e-106 311
Msa1421790 AT4G33260.2 69.683 221 38 1 1 192 210 430 9.63e-106 311
Msa1421790 AT5G27080.1 64.583 240 54 3 1 209 203 442 1.88e-102 303
Msa1421790 AT5G26900.1 64.435 239 55 2 1 209 206 444 3.33e-102 302
Msa1421790 AT5G13840.1 48.792 207 83 2 3 186 254 460 3.56e-66 211
Msa1421790 AT5G13840.2 48.792 207 83 2 3 186 254 460 3.56e-66 211
Msa1421790 AT4G22910.1 47.343 207 86 1 4 187 257 463 1.68e-64 207
Msa1421790 AT4G11920.1 44.860 214 95 1 4 194 249 462 5.49e-62 200

Find 63 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATGAAACTCTTAGATTT+TGG 0.143277 tig0025298:-37831 Msa1421790:CDS
GTGGCTTTGCATGCTTCTGC+TGG 0.230906 tig0025298:+37793 Msa1421790:intergenic
CCTTTGGAAGTATCCTTCTA+TGG 0.254046 tig0025298:-37941 Msa1421790:CDS
CAGGGTAATCTACTGGCTTC+TGG 0.309255 tig0025298:-38120 Msa1421790:CDS
GTATCCTTCTATGGTGAAAA+TGG 0.311467 tig0025298:-37932 Msa1421790:CDS
AAGGGCTCATTATATGTCTT+TGG 0.358151 tig0025298:+37769 Msa1421790:intergenic
ACGATAATGTTGTTCACATA+TGG 0.371186 tig0025298:-38237 Msa1421790:CDS
AATCTACTGGCTTCTGGTGG+AGG 0.376592 tig0025298:-38114 Msa1421790:CDS
GTCATCATCTTCAACGAATA+CGG 0.384451 tig0025298:+37734 Msa1421790:intergenic
TTAGTAGCTCACGCTCATTC+TGG 0.385492 tig0025298:+37995 Msa1421790:intergenic
CACTTCGAGGGTGATGTATA+TGG 0.388915 tig0025298:-37893 Msa1421790:CDS
TGGCTTTGCATGCTTCTGCT+GGG 0.422084 tig0025298:+37794 Msa1421790:intergenic
CCGAGAACCAGCTTACCCTT+TGG 0.427049 tig0025298:-37957 Msa1421790:CDS
CTCACATCGTTGAAACATAC+AGG 0.428220 tig0025298:-38327 Msa1421790:CDS
CTACTGGCTTCTGGTGGAGG+TGG 0.431389 tig0025298:-38111 Msa1421790:CDS
AACAATCATATTCTGACAAC+AGG 0.439142 tig0025298:-38394 Msa1421790:CDS
CTGGCTTCTGGTGGAGGTGG+AGG 0.440261 tig0025298:-38108 Msa1421790:CDS
CAAGAGTGGGTTCACTGTCT+TGG 0.449748 tig0025298:-38417 Msa1421790:CDS
CGCAAGTATGTGCTCTGCTA+TGG 0.473307 tig0025298:-38020 Msa1421790:CDS
GATGCCACTGTACACCCATC+TGG 0.480407 tig0025298:+37862 Msa1421790:intergenic
ATCTCAACGGTCACACTTCG+AGG 0.480528 tig0025298:-37906 Msa1421790:CDS
CCAAAGGGTAAGCTGGTTCT+CGG 0.481991 tig0025298:+37957 Msa1421790:intergenic
CGATAATGTTGTTCACATAT+GGG 0.487900 tig0025298:-38236 Msa1421790:CDS
TGTCCCTCAGGGTAATCTAC+TGG 0.491265 tig0025298:-38127 Msa1421790:intron
GGACACAACCAGGAGGTTTG+TGG 0.497262 tig0025298:-38304 Msa1421790:CDS
GATACTTCCAAAGGGTAAGC+TGG 0.503616 tig0025298:+37950 Msa1421790:intergenic
GACACAACCAGGAGGTTTGT+GGG 0.510860 tig0025298:-38303 Msa1421790:CDS
AACATACAGGGGACACAACC+AGG 0.511067 tig0025298:-38314 Msa1421790:CDS
CTCATTATATGTCTTTGGTG+TGG 0.516280 tig0025298:+37774 Msa1421790:intergenic
TATTCTGACAACAGGAGGAA+TGG 0.524698 tig0025298:-38386 Msa1421790:CDS
GGGCTCAAGTGGTCACCCTC+AGG 0.528586 tig0025298:-38283 Msa1421790:CDS
AGGAGGTTTGTGGGCTCAAG+TGG 0.528883 tig0025298:-38294 Msa1421790:CDS
ACTTGAGCCCACAAACCTCC+TGG 0.538177 tig0025298:+38296 Msa1421790:intergenic
TCATCATCTTCAACGAATAC+GGG 0.547121 tig0025298:+37735 Msa1421790:intergenic
GGGTGGACACAGAGCAAGAG+TGG 0.549117 tig0025298:-38431 Msa1421790:CDS
GGTAATCTACTGGCTTCTGG+TGG 0.565300 tig0025298:-38117 Msa1421790:CDS
TATATGGCGCAAAGTCCAGA+TGG 0.565373 tig0025298:-37877 Msa1421790:CDS
CGTGAGCTACTAAGCTCACA+TGG 0.568122 tig0025298:-37985 Msa1421790:CDS
CTGACAACAGGAGGAATGGA+TGG 0.572412 tig0025298:-38382 Msa1421790:CDS
CATAGAAGGATACTTCCAAA+GGG 0.584766 tig0025298:+37942 Msa1421790:intergenic
TCACATATGGGATAGATCTG+TGG 0.596581 tig0025298:-38224 Msa1421790:intron
AGATTGAATTCAGTTGACAC+TGG 0.597762 tig0025298:-38045 Msa1421790:CDS
GGACAACAATTGTCAAGTGG+TGG 0.599505 tig0025298:-38262 Msa1421790:CDS
ATACGGGTGAAATTAGCAAA+GGG 0.600657 tig0025298:+37751 Msa1421790:intergenic
TCTCAACGGTCACACTTCGA+GGG 0.613321 tig0025298:-37905 Msa1421790:CDS
GTGAAAATGGCAGATCTCAA+CGG 0.613838 tig0025298:-37919 Msa1421790:CDS
AATACGGGTGAAATTAGCAA+AGG 0.619999 tig0025298:+37750 None:intergenic
CAGCTAAGGGCAATGAAGGG+TGG 0.622963 tig0025298:-38448 None:intergenic
CCATAGAAGGATACTTCCAA+AGG 0.623454 tig0025298:+37941 Msa1421790:intergenic
AAGCTGTGGAACACACACAC+TGG 0.630912 tig0025298:-38072 Msa1421790:CDS
GTGATCATTGCATTAAGCTG+TGG 0.635919 tig0025298:-38086 Msa1421790:CDS
AATCATATTCTGACAACAGG+AGG 0.641326 tig0025298:-38391 Msa1421790:CDS
ACTTGACAATTGTTGTCCTG+AGG 0.654097 tig0025298:+38267 Msa1421790:intergenic
TCACATCGTTGAAACATACA+GGG 0.656486 tig0025298:-38326 Msa1421790:CDS
CTTGACAATTGTTGTCCTGA+GGG 0.657303 tig0025298:+38268 Msa1421790:intergenic
TCAGGACAACAATTGTCAAG+TGG 0.664718 tig0025298:-38265 Msa1421790:CDS
TGGGTGTACAGTGGCATCTG+CGG 0.666837 tig0025298:-37857 Msa1421790:CDS
CTGCAGCTAAGGGCAATGAA+GGG 0.668716 tig0025298:-38451 None:intergenic
GGTGGACACAGAGCAAGAGT+GGG 0.681424 tig0025298:-38430 Msa1421790:CDS
AAGTCCAGATGGGTGTACAG+TGG 0.686450 tig0025298:-37866 Msa1421790:CDS
ATATGGCGCAAAGTCCAGAT+GGG 0.692355 tig0025298:-37876 Msa1421790:CDS
CACATCGTTGAAACATACAG+GGG 0.758385 tig0025298:-38325 Msa1421790:CDS
ATACAGGGGACACAACCAGG+AGG 0.774768 tig0025298:-38311 Msa1421790:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGATTTTGGAATGTCTTT+GGG - tig0025298:38361-38380 Msa1421790:CDS 25.0%
AACAATCATATTCTGACAAC+AGG - tig0025298:37786-37805 Msa1421790:CDS 30.0%
ACGATAATGTTGTTCACATA+TGG - tig0025298:37943-37962 Msa1421790:CDS 30.0%
CGATAATGTTGTTCACATAT+GGG - tig0025298:37944-37963 Msa1421790:CDS 30.0%
! CAGATGAAACTCTTAGATTT+TGG - tig0025298:38349-38368 Msa1421790:CDS 30.0%
!!! CTTAGATTTTGGAATGTCTT+TGG - tig0025298:38360-38379 Msa1421790:CDS 30.0%
AATCATATTCTGACAACAGG+AGG - tig0025298:37789-37808 Msa1421790:CDS 35.0%
TCACATCGTTGAAACATACA+GGG - tig0025298:37854-37873 Msa1421790:CDS 35.0%
AGATTGAATTCAGTTGACAC+TGG - tig0025298:38135-38154 Msa1421790:intron 35.0%
CATAGAAGGATACTTCCAAA+GGG + tig0025298:38241-38260 Msa1421790:intergenic 35.0%
GTATCCTTCTATGGTGAAAA+TGG - tig0025298:38248-38267 Msa1421790:CDS 35.0%
CTCATTATATGTCTTTGGTG+TGG + tig0025298:38409-38428 Msa1421790:intergenic 35.0%
AAGGGCTCATTATATGTCTT+TGG + tig0025298:38414-38433 Msa1421790:intergenic 35.0%
ATACGGGTGAAATTAGCAAA+GGG + tig0025298:38432-38451 Msa1421790:intergenic 35.0%
AATACGGGTGAAATTAGCAA+AGG + tig0025298:38433-38452 Msa1421790:intergenic 35.0%
TATTCTGACAACAGGAGGAA+TGG - tig0025298:37794-37813 Msa1421790:CDS 40.0%
CTCACATCGTTGAAACATAC+AGG - tig0025298:37853-37872 Msa1421790:CDS 40.0%
CACATCGTTGAAACATACAG+GGG - tig0025298:37855-37874 Msa1421790:CDS 40.0%
CTTGACAATTGTTGTCCTGA+GGG + tig0025298:37915-37934 Msa1421790:intergenic 40.0%
ACTTGACAATTGTTGTCCTG+AGG + tig0025298:37916-37935 Msa1421790:intergenic 40.0%
TCAGGACAACAATTGTCAAG+TGG - tig0025298:37915-37934 Msa1421790:CDS 40.0%
TCACATATGGGATAGATCTG+TGG - tig0025298:37956-37975 Msa1421790:CDS 40.0%
GTGATCATTGCATTAAGCTG+TGG - tig0025298:38094-38113 Msa1421790:CDS 40.0%
CCATAGAAGGATACTTCCAA+AGG + tig0025298:38242-38261 Msa1421790:intergenic 40.0%
!! CCTTTGGAAGTATCCTTCTA+TGG - tig0025298:38239-38258 Msa1421790:CDS 40.0%
!!! TCTGCCATTTTCACCATAGA+AGG + tig0025298:38255-38274 Msa1421790:intergenic 40.0%
GTGAAAATGGCAGATCTCAA+CGG - tig0025298:38261-38280 Msa1421790:CDS 40.0%
GGACAACAATTGTCAAGTGG+TGG - tig0025298:37918-37937 Msa1421790:CDS 45.0%
CTCAAACCTGTGAAGACATC+GGG + tig0025298:38000-38019 Msa1421790:intergenic 45.0%
CCTCAAACCTGTGAAGACAT+CGG + tig0025298:38001-38020 Msa1421790:intergenic 45.0%
AAGCCAGTAGATTACCCTGA+GGG + tig0025298:38059-38078 Msa1421790:intergenic 45.0%
TTAGTAGCTCACGCTCATTC+TGG + tig0025298:38188-38207 Msa1421790:intergenic 45.0%
GATACTTCCAAAGGGTAAGC+TGG + tig0025298:38233-38252 Msa1421790:intergenic 45.0%
CACTTCGAGGGTGATGTATA+TGG - tig0025298:38287-38306 Msa1421790:CDS 45.0%
TATATGGCGCAAAGTCCAGA+TGG - tig0025298:38303-38322 Msa1421790:CDS 45.0%
ATATGGCGCAAAGTCCAGAT+GGG - tig0025298:38304-38323 Msa1421790:CDS 45.0%
! CAAGAGTGGGTTCACTGTCT+TGG - tig0025298:37763-37782 Msa1421790:CDS 50.0%
CTGACAACAGGAGGAATGGA+TGG - tig0025298:37798-37817 Msa1421790:CDS 50.0%
AACATACAGGGGACACAACC+AGG - tig0025298:37866-37885 Msa1421790:CDS 50.0%
GACACAACCAGGAGGTTTGT+GGG - tig0025298:37877-37896 Msa1421790:CDS 50.0%
AACCTGTGAAGACATCGGGT+AGG + tig0025298:37996-38015 Msa1421790:intergenic 50.0%
CCGATGTCTTCACAGGTTTG+AGG - tig0025298:37998-38017 Msa1421790:CDS 50.0%
GGAACACATAGCTGCTGTGA+AGG - tig0025298:38019-38038 Msa1421790:CDS 50.0%
TGTCCCTCAGGGTAATCTAC+TGG - tig0025298:38053-38072 Msa1421790:CDS 50.0%
GAAGCCAGTAGATTACCCTG+AGG + tig0025298:38060-38079 Msa1421790:intergenic 50.0%
!! CAGGGTAATCTACTGGCTTC+TGG - tig0025298:38060-38079 Msa1421790:CDS 50.0%
!! GGTAATCTACTGGCTTCTGG+TGG - tig0025298:38063-38082 Msa1421790:CDS 50.0%
!! AATCTACTGGCTTCTGGTGG+AGG - tig0025298:38066-38085 Msa1421790:CDS 50.0%
AAGCTGTGGAACACACACAC+TGG - tig0025298:38108-38127 Msa1421790:CDS 50.0%
CGCAAGTATGTGCTCTGCTA+TGG - tig0025298:38160-38179 Msa1421790:intron 50.0%
CGTGAGCTACTAAGCTCACA+TGG - tig0025298:38195-38214 Msa1421790:intron 50.0%
! CCAAAGGGTAAGCTGGTTCT+CGG + tig0025298:38226-38245 Msa1421790:intergenic 50.0%
ATCTCAACGGTCACACTTCG+AGG - tig0025298:38274-38293 Msa1421790:CDS 50.0%
TCTCAACGGTCACACTTCGA+GGG - tig0025298:38275-38294 Msa1421790:CDS 50.0%
AAGTCCAGATGGGTGTACAG+TGG - tig0025298:38314-38333 Msa1421790:CDS 50.0%
! TGGCTTTGCATGCTTCTGCT+GGG + tig0025298:38389-38408 Msa1421790:intergenic 50.0%
GGTGGACACAGAGCAAGAGT+GGG - tig0025298:37750-37769 Msa1421790:CDS 55.0%
ATACAGGGGACACAACCAGG+AGG - tig0025298:37869-37888 Msa1421790:CDS 55.0%
GGACACAACCAGGAGGTTTG+TGG - tig0025298:37876-37895 Msa1421790:CDS 55.0%
ACTTGAGCCCACAAACCTCC+TGG + tig0025298:37887-37906 Msa1421790:intergenic 55.0%
AGGAGGTTTGTGGGCTCAAG+TGG - tig0025298:37886-37905 Msa1421790:CDS 55.0%
CACCTACCCGATGTCTTCAC+AGG - tig0025298:37991-38010 Msa1421790:CDS 55.0%
! CATAGCTGCTGTGAAGGCAC+TGG - tig0025298:38025-38044 Msa1421790:CDS 55.0%
CCGAGAACCAGCTTACCCTT+TGG - tig0025298:38223-38242 Msa1421790:intron 55.0%
GATGCCACTGTACACCCATC+TGG + tig0025298:38321-38340 Msa1421790:intergenic 55.0%
TGGGTGTACAGTGGCATCTG+CGG - tig0025298:38323-38342 Msa1421790:CDS 55.0%
! GTGGCTTTGCATGCTTCTGC+TGG + tig0025298:38390-38409 Msa1421790:intergenic 55.0%
GGGTGGACACAGAGCAAGAG+TGG - tig0025298:37749-37768 Msa1421790:CDS 60.0%
!! CTGCTGTGAAGGCACTGGCT+TGG - tig0025298:38030-38049 Msa1421790:CDS 60.0%
!! CACTGGCTTGGTGTCCCTCA+GGG - tig0025298:38042-38061 Msa1421790:CDS 60.0%
!! CTACTGGCTTCTGGTGGAGG+TGG - tig0025298:38069-38088 Msa1421790:CDS 60.0%
GGGCTCAAGTGGTCACCCTC+AGG - tig0025298:37897-37916 Msa1421790:CDS 65.0%
!! GCACTGGCTTGGTGTCCCTC+AGG - tig0025298:38041-38060 Msa1421790:CDS 65.0%
CTGGCTTCTGGTGGAGGTGG+AGG - tig0025298:38072-38091 Msa1421790:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0025298 gene 37744 38458 37744 ID=Msa1421790;Name=Msa1421790
tig0025298 mRNA 37744 38458 37744 ID=Msa1421790-mRNA-1;Parent=Msa1421790;Name=Msa1421790-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|1|1|2|0|209
tig0025298 exon 38225 38458 38225 ID=Msa1421790-mRNA-1:exon:2242;Parent=Msa1421790-mRNA-1
tig0025298 exon 37744 38139 37744 ID=Msa1421790-mRNA-1:exon:2241;Parent=Msa1421790-mRNA-1
tig0025298 CDS 38225 38458 38225 ID=Msa1421790-mRNA-1:cds;Parent=Msa1421790-mRNA-1
tig0025298 CDS 37744 38139 37744 ID=Msa1421790-mRNA-1:cds;Parent=Msa1421790-mRNA-1
Gene Sequence

>Msa1421790

ATGAAGGGTGGACACAGAGCAAGAGTGGGTTCACTGTCTTGGAACAATCATATTCTGACAACAGGAGGAATGGATGGTAAAGTTGTAAACAATGATGTTAGAGTGAGATCTCACATCGTTGAAACATACAGGGGACACAACCAGGAGGTTTGTGGGCTCAAGTGGTCACCCTCAGGACAACAATTGTCAAGTGGTGGAAACGATAATGTTGTTCACATATGGGATAGATCTGTGGGTAATCTACTGGCTTCTGGTGGAGGTGGAGGTGATCATTGCATTAAGCTGTGGAACACACACACTGGTGCGAGATTGAATTCAGTTGACACTGGATCGCAAGTATGTGCTCTGCTATGGAACCAGAATGAGCGTGAGCTACTAAGCTCACATGGCTTCACCGAGAACCAGCTTACCCTTTGGAAGTATCCTTCTATGGTGAAAATGGCAGATCTCAACGGTCACACTTCGAGGGTGATGTATATGGCGCAAAGTCCAGATGGGTGTACAGTGGCATCTGCGGCTGCAGATGAAACTCTTAGATTTTGGAATGTCTTTGGGAGCCCAGCAGAAGCATGCAAAGCCACACCAAAGACATATAATGAGCCCTTTGCTAATTTCACCCGTATTCGTTGA

Protein sequence

>Msa1421790

MKGGHRARVGSLSWNNHILTTGGMDGKVVNNDVRVRSHIVETYRGHNQEVCGLKWSPSGQQLSSGGNDNVVHIWDRSVGNLLASGGGGGDHCIKLWNTHTGARLNSVDTGSQVCALLWNQNERELLSSHGFTENQLTLWKYPSMVKMADLNGHTSRVMYMAQSPDGCTVASAAADETLRFWNVFGSPAEACKATPKTYNEPFANFTRIR*