AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1165260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1165260 MtrunA17_Chr7g0271861 82.639 144 10 1 1 144 1 129 9.28e-83 239
Msa1165260 MtrunA17_Chr4g0068031 46.457 127 54 4 21 144 29 144 2.17e-25 94.4
Msa1165260 MtrunA17_Chr1g0213161 54.348 46 20 1 14 59 145 189 2.20e-13 64.7
Msa1165260 MtrunA17_Chr1g0151461 72.222 36 10 0 22 57 187 222 3.46e-13 65.1
Msa1165260 MtrunA17_Chr2g0326771 57.778 45 19 0 13 57 207 251 7.48e-13 64.3
Msa1165260 MtrunA17_Chr5g0404131 60.526 38 15 0 18 55 188 225 3.97e-12 62.0
Msa1165260 MtrunA17_Chr4g0071301 80.000 30 6 0 22 51 192 221 4.17e-12 62.0
Msa1165260 MtrunA17_Chr8g0373231 62.162 37 14 0 19 55 122 158 4.58e-12 61.2
Msa1165260 MtrunA17_Chr2g0299081 76.667 30 7 0 22 51 169 198 8.39e-12 61.2
Msa1165260 MtrunA17_Chr1g0146231 70.588 34 10 0 24 57 236 269 3.24e-11 59.7
Msa1165260 MtrunA17_Chr4g0028961 49.091 55 27 1 3 57 214 267 3.42e-11 59.7
Msa1165260 MtrunA17_Chr1g0199671 67.647 34 11 0 24 57 244 277 7.94e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1165260 AT5G49300.1 50.000 124 43 2 21 144 35 139 2.62e-29 104
Msa1165260 AT3G06740.1 46.923 130 43 4 21 144 40 149 6.46e-28 101
Msa1165260 AT3G16870.1 38.667 150 66 3 21 144 41 190 6.03e-19 79.3
Msa1165260 AT5G56860.1 78.947 38 7 1 18 55 227 263 2.81e-14 68.9
Msa1165260 AT4G16141.1 72.973 37 10 0 21 57 36 72 9.90e-14 65.5
Msa1165260 AT4G26150.1 75.758 33 8 0 20 52 197 229 4.58e-13 65.5
Msa1165260 AT4G36620.1 39.726 73 29 1 18 90 71 128 1.13e-12 63.2
Msa1165260 AT5G26930.1 40.000 120 43 4 15 134 20 110 1.15e-12 61.2
Msa1165260 AT1G08010.2 65.000 40 13 1 18 57 217 255 1.44e-12 63.5
Msa1165260 AT1G08010.1 65.000 40 13 1 18 57 217 255 1.44e-12 63.5
Msa1165260 AT1G08010.3 65.000 40 13 1 18 57 217 255 1.44e-12 63.5
Msa1165260 AT2G18380.1 52.941 51 21 1 8 55 75 125 2.46e-12 62.0
Msa1165260 AT3G50870.1 54.167 48 20 1 19 64 149 196 8.07e-12 61.6
Msa1165260 AT3G45170.1 64.103 39 14 0 16 54 109 147 7.04e-11 58.2
Msa1165260 AT5G25830.1 53.061 49 23 0 9 57 206 254 9.20e-11 58.9

Find 66 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAGAAAGAGAGAGAAAA+TGG 0.255654 7_4:+57104301 None:intergenic
TCACAACACTGAAACAATAA+TGG 0.274232 7_4:+57104178 None:intergenic
ACTTAGTTCCAAATCTTGTT+AGG 0.278264 7_4:-57103149 Msa1165260:three_prime_UTR
CCCTAGTCCCTAACAAGATT+TGG 0.304180 7_4:+57103141 None:intergenic
TCCTCTCCAAAGTGGTGTTT+TGG 0.317787 7_4:+57103947 None:intergenic
AATGATACCTTGGGTCCATT+TGG 0.368205 7_4:+57103921 None:intergenic
TTTCGTTTCGGTGTGTGATT+CGG 0.397003 7_4:-57104247 Msa1165260:five_prime_UTR
ACAAGAAAGGGAAAGGGAAA+GGG 0.399518 7_4:-57103588 Msa1165260:CDS
CACAACACTGAAACAATAAT+GGG 0.400210 7_4:+57104179 None:intergenic
AATGGGAATCATGGATCAAA+TGG 0.411033 7_4:-57104151 Msa1165260:CDS
ATGTTGTTCATACGATGAAA+TGG 0.428480 7_4:-57104006 Msa1165260:CDS
AGGGAAAGGAAGAGAGAGAA+AGG 0.432833 7_4:-57103569 Msa1165260:CDS
CTTAGTTCCAAATCTTGTTA+GGG 0.440631 7_4:-57103148 Msa1165260:three_prime_UTR
GCACAGAATGGGAATGGGAG+GGG 0.445124 7_4:-57103545 Msa1165260:CDS
TGTGCAACGCTTGTGGAATC+AGG 0.447660 7_4:-57103643 Msa1165260:CDS
GAAAATGAAGTTAGTGGCAT+TGG 0.448903 7_4:-57103471 Msa1165260:CDS
AAAGGGAAAGGGAAAGGGAA+AGG 0.449906 7_4:-57103583 Msa1165260:CDS
TCCAAATCTTGTTAGGGACT+AGG 0.460368 7_4:-57103142 Msa1165260:three_prime_UTR
TTTCAGTGTTGTGAATCAAA+TGG 0.461831 7_4:-57104169 Msa1165260:exon
AATATTAAAATGTTAGAAGA+AGG 0.462964 7_4:-57103328 Msa1165260:three_prime_UTR
AGTAGAAACAAGTTTCGTTT+CGG 0.463442 7_4:-57104259 Msa1165260:five_prime_UTR
GACAAGAAAGGGAAAGGGAA+AGG 0.465040 7_4:-57103589 Msa1165260:CDS
CCAAATCTTGTTAGGGACTA+GGG 0.469956 7_4:-57103141 Msa1165260:three_prime_UTR
GAGAGAAAGGTGCACAGAAT+GGG 0.472351 7_4:-57103556 Msa1165260:CDS
ACTGAAACAATAATGGGTTA+AGG 0.476837 7_4:+57104185 None:intergenic
AATCATGGATCAAATGGATA+AGG 0.481862 7_4:-57104145 Msa1165260:intron
AGAGAGAAAGGTGCACAGAA+TGG 0.485338 7_4:-57103557 Msa1165260:CDS
AAAATGAAGTTAGTGGCATT+GGG 0.487144 7_4:-57103470 Msa1165260:CDS
GTGTTTGAAAATGAAGTTAG+TGG 0.497670 7_4:-57103477 Msa1165260:CDS
AAAGGTGCACAGAATGGGAA+TGG 0.500630 7_4:-57103551 Msa1165260:CDS
TGTTGCAGAAGAAACAAAGA+AGG 0.501168 7_4:-57103436 Msa1165260:CDS
CTTTGGAGAGGAGGACCAAA+TGG 0.503109 7_4:-57103936 Msa1165260:CDS
TTCAGTGTTGTGAATCAAAT+GGG 0.509228 7_4:-57104168 Msa1165260:exon
TGTGAATCAAATGGGAATCA+TGG 0.514991 7_4:-57104160 Msa1165260:exon
TGTTTGATGGCACTCTCTTG+TGG 0.524330 7_4:-57103380 Msa1165260:CDS
ATATTAAAATGTTAGAAGAA+GGG 0.525100 7_4:-57103327 Msa1165260:three_prime_UTR
TATTAAAATGTTAGAAGAAG+GGG 0.528171 7_4:-57103326 Msa1165260:three_prime_UTR
TTGTAGCAAAGGACAAGAAA+GGG 0.531317 7_4:-57103600 Msa1165260:CDS
AAACCACCAAAACACCACTT+TGG 0.542523 7_4:-57103953 Msa1165260:CDS
ACAAGCTGCTGTGTGTTTGA+TGG 0.545240 7_4:-57103393 Msa1165260:CDS
ATTAAAATGTTAGAAGAAGG+GGG 0.547323 7_4:-57103325 Msa1165260:three_prime_UTR
GTTGTAGCAAAGGACAAGAA+AGG 0.552771 7_4:-57103601 Msa1165260:CDS
CAAAGGACAAGAAAGGGAAA+GGG 0.557048 7_4:-57103594 Msa1165260:CDS
TAAATATGAACGATACGAAA+CGG 0.558626 7_4:+57104218 None:intergenic
TGTTGTTCATACGATGAAAT+GGG 0.559954 7_4:-57104005 Msa1165260:CDS
GCAAAGGACAAGAAAGGGAA+AGG 0.569001 7_4:-57103595 Msa1165260:CDS
AAGGATTAAACTAGGTGAAG+AGG 0.572329 7_4:-57103417 Msa1165260:CDS
AAGGTGCACAGAATGGGAAT+GGG 0.575912 7_4:-57103550 Msa1165260:CDS
AATCAGGTACAGAAAGAGAA+GGG 0.588011 7_4:-57103627 Msa1165260:CDS
AGAAGGGGTTGTTGTAGCAA+AGG 0.588421 7_4:-57103611 Msa1165260:CDS
TTAAAATGTTAGAAGAAGGG+GGG 0.591566 7_4:-57103324 Msa1165260:three_prime_UTR
GTGCACAGAATGGGAATGGG+AGG 0.593014 7_4:-57103547 Msa1165260:CDS
TAAAATGTTAGAAGAAGGGG+GGG 0.596898 7_4:-57103323 Msa1165260:three_prime_UTR
CAGAATGGGAATGGGAGGGG+TGG 0.600363 7_4:-57103542 Msa1165260:CDS
TAGACGTTGTGCAACGCTTG+TGG 0.606961 7_4:-57103650 Msa1165260:CDS
TGCACAGAATGGGAATGGGA+GGG 0.611263 7_4:-57103546 Msa1165260:CDS
AAACAAAGAAGGATTAAACT+AGG 0.611388 7_4:-57103425 Msa1165260:CDS
GGTGGTACTAGCAGTAGCAG+TGG 0.618851 7_4:-57103524 Msa1165260:CDS
GAATCAGGTACAGAAAGAGA+AGG 0.619622 7_4:-57103628 Msa1165260:CDS
TTTGGTCCTCCTCTCCAAAG+TGG 0.621363 7_4:+57103939 None:intergenic
AAAACACCACTTTGGAGAGG+AGG 0.625709 7_4:-57103945 Msa1165260:CDS
AGGAGGACCAAATGGACCCA+AGG 0.633971 7_4:-57103928 Msa1165260:intron
TTGTTAGGGACTAGGGAGTG+AGG 0.653298 7_4:-57103134 Msa1165260:three_prime_UTR
TTAGGGACTAGGGAGTGAGG+TGG 0.666308 7_4:-57103131 Msa1165260:three_prime_UTR
ACCAAAACACCACTTTGGAG+AGG 0.669357 7_4:-57103948 Msa1165260:CDS
ATCAGGTACAGAAAGAGAAG+GGG 0.680743 7_4:-57103626 Msa1165260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTTTATTAATTTGATTAA+AGG - chr7_4:57103679-57103698 Msa1165260:intron 10.0%
!!! TGTTTATTAATTTGATTAAA+GGG - chr7_4:57103680-57103699 Msa1165260:intron 10.0%
!!! ATGTTTATTAATTTGATTAA+AGG - chr7_4:57103679-57103698 Msa1165260:intron 10.0%
!!! TGTTTATTAATTTGATTAAA+GGG - chr7_4:57103680-57103699 Msa1165260:intron 10.0%
!! AAAAAAAAAAATGATACCTT+GGG + chr7_4:57103508-57103527 None:intergenic 15.0%
!! AAAAAAAAAAAATGATACCT+TGG + chr7_4:57103509-57103528 None:intergenic 15.0%
!!! GTTTTCAAATATAGATAAAA+TGG + chr7_4:57103587-57103606 None:intergenic 15.0%
!! AAGTGAATAATATATCAAAT+TGG + chr7_4:57103615-57103634 None:intergenic 15.0%
!! AAACAATAATCATATCAAAA+AGG + chr7_4:57103644-57103663 None:intergenic 15.0%
!!! TGATTTTTTTTTGTTTTTGT+TGG - chr7_4:57103738-57103757 Msa1165260:intron 15.0%
!!! GATTTTTTTTTGTTTTTGTT+GGG - chr7_4:57103739-57103758 Msa1165260:intron 15.0%
!! AATATTAAAATGTTAGAAGA+AGG - chr7_4:57104089-57104108 Msa1165260:intron 15.0%
!! ATATTAAAATGTTAGAAGAA+GGG - chr7_4:57104090-57104109 Msa1165260:intron 15.0%
!! AAAAAAAAAAATGATACCTT+GGG + chr7_4:57103508-57103527 None:intergenic 15.0%
!! AAAAAAAAAAAATGATACCT+TGG + chr7_4:57103509-57103528 None:intergenic 15.0%
!!! GTTTTCAAATATAGATAAAA+TGG + chr7_4:57103587-57103606 None:intergenic 15.0%
!! AAGTGAATAATATATCAAAT+TGG + chr7_4:57103615-57103634 None:intergenic 15.0%
!! AAACAATAATCATATCAAAA+AGG + chr7_4:57103644-57103663 None:intergenic 15.0%
!!! TGATTTTTTTTTGTTTTTGT+TGG - chr7_4:57103738-57103757 Msa1165260:intron 15.0%
!!! GATTTTTTTTTGTTTTTGTT+GGG - chr7_4:57103739-57103758 Msa1165260:intron 15.0%
!! AATATTAAAATGTTAGAAGA+AGG - chr7_4:57104089-57104108 Msa1165260:intron 15.0%
!! ATATTAAAATGTTAGAAGAA+GGG - chr7_4:57104090-57104109 Msa1165260:intron 15.0%
!! AAGTTACAAAATGATGAAAT+GGG + chr7_4:57103538-57103557 None:intergenic 20.0%
!! TAAGTTACAAAATGATGAAA+TGG + chr7_4:57103539-57103558 None:intergenic 20.0%
!! TGAATAATATATCAAATTGG+TGG + chr7_4:57103612-57103631 None:intergenic 20.0%
!! TATTAAAATGTTAGAAGAAG+GGG - chr7_4:57104091-57104110 Msa1165260:intron 20.0%
!!! CTTTCTTTAGTTTTTAGAAA+GGG - chr7_4:57104233-57104252 Msa1165260:five_prime_UTR 20.0%
!! AAGTTACAAAATGATGAAAT+GGG + chr7_4:57103538-57103557 None:intergenic 20.0%
!! TAAGTTACAAAATGATGAAA+TGG + chr7_4:57103539-57103558 None:intergenic 20.0%
!! TGAATAATATATCAAATTGG+TGG + chr7_4:57103612-57103631 None:intergenic 20.0%
!! TATTAAAATGTTAGAAGAAG+GGG - chr7_4:57104091-57104110 Msa1165260:intron 20.0%
!!! CTTTCTTTAGTTTTTAGAAA+GGG - chr7_4:57104233-57104252 Msa1165260:five_prime_UTR 20.0%
! TAAATATGAACGATACGAAA+CGG + chr7_4:57103202-57103221 None:intergenic 25.0%
! CTTCTTTCTATTTGTTTAAC+AGG - chr7_4:57103387-57103406 Msa1165260:CDS 25.0%
! AGATAAAATGGAAGTGAAAA+AGG + chr7_4:57103575-57103594 None:intergenic 25.0%
! AAACAAAGAAGGATTAAACT+AGG - chr7_4:57103992-57104011 Msa1165260:CDS 25.0%
! ATTAAAATGTTAGAAGAAGG+GGG - chr7_4:57104092-57104111 Msa1165260:intron 25.0%
! AAAGAGAGAAAACAAAAAAG+AGG + chr7_4:57104180-57104199 None:intergenic 25.0%
!!! TTTTGTTTTCTCTCTTTCTT+TGG - chr7_4:57104183-57104202 Msa1165260:five_prime_UTR 25.0%
!! CCTTTCTAAAAACTAAAGAA+AGG + chr7_4:57104235-57104254 None:intergenic 25.0%
!!! CCTTTCTTTAGTTTTTAGAA+AGG - chr7_4:57104232-57104251 Msa1165260:five_prime_UTR 25.0%
! TAAATATGAACGATACGAAA+CGG + chr7_4:57103202-57103221 None:intergenic 25.0%
! CTTCTTTCTATTTGTTTAAC+AGG - chr7_4:57103387-57103406 Msa1165260:CDS 25.0%
! AGATAAAATGGAAGTGAAAA+AGG + chr7_4:57103575-57103594 None:intergenic 25.0%
! AAACAAAGAAGGATTAAACT+AGG - chr7_4:57103992-57104011 Msa1165260:CDS 25.0%
! ATTAAAATGTTAGAAGAAGG+GGG - chr7_4:57104092-57104111 Msa1165260:intron 25.0%
! AAAGAGAGAAAACAAAAAAG+AGG + chr7_4:57104180-57104199 None:intergenic 25.0%
!!! TTTTGTTTTCTCTCTTTCTT+TGG - chr7_4:57104183-57104202 Msa1165260:five_prime_UTR 25.0%
!! CCTTTCTAAAAACTAAAGAA+AGG + chr7_4:57104235-57104254 None:intergenic 25.0%
!!! CCTTTCTTTAGTTTTTAGAA+AGG - chr7_4:57104232-57104251 Msa1165260:five_prime_UTR 25.0%
AGTAGAAACAAGTTTCGTTT+CGG - chr7_4:57103158-57103177 Msa1165260:three_prime_UTR 30.0%
!! ACTGAAACAATAATGGGTTA+AGG + chr7_4:57103235-57103254 None:intergenic 30.0%
CACAACACTGAAACAATAAT+GGG + chr7_4:57103241-57103260 None:intergenic 30.0%
TCACAACACTGAAACAATAA+TGG + chr7_4:57103242-57103261 None:intergenic 30.0%
TTTCAGTGTTGTGAATCAAA+TGG - chr7_4:57103248-57103267 Msa1165260:three_prime_UTR 30.0%
TTCAGTGTTGTGAATCAAAT+GGG - chr7_4:57103249-57103268 Msa1165260:three_prime_UTR 30.0%
AATCATGGATCAAATGGATA+AGG - chr7_4:57103272-57103291 Msa1165260:three_prime_UTR 30.0%
ATGTTGTTCATACGATGAAA+TGG - chr7_4:57103411-57103430 Msa1165260:CDS 30.0%
TGTTGTTCATACGATGAAAT+GGG - chr7_4:57103412-57103431 Msa1165260:CDS 30.0%
GTGTTTGAAAATGAAGTTAG+TGG - chr7_4:57103940-57103959 Msa1165260:CDS 30.0%
AAAATGAAGTTAGTGGCATT+GGG - chr7_4:57103947-57103966 Msa1165260:CDS 30.0%
TTAAAATGTTAGAAGAAGGG+GGG - chr7_4:57104093-57104112 Msa1165260:intron 30.0%
ACTTAGTTCCAAATCTTGTT+AGG - chr7_4:57104268-57104287 Msa1165260:five_prime_UTR 30.0%
CTTAGTTCCAAATCTTGTTA+GGG - chr7_4:57104269-57104288 Msa1165260:five_prime_UTR 30.0%
AGTAGAAACAAGTTTCGTTT+CGG - chr7_4:57103158-57103177 Msa1165260:three_prime_UTR 30.0%
!! ACTGAAACAATAATGGGTTA+AGG + chr7_4:57103235-57103254 None:intergenic 30.0%
CACAACACTGAAACAATAAT+GGG + chr7_4:57103241-57103260 None:intergenic 30.0%
TCACAACACTGAAACAATAA+TGG + chr7_4:57103242-57103261 None:intergenic 30.0%
TTTCAGTGTTGTGAATCAAA+TGG - chr7_4:57103248-57103267 Msa1165260:three_prime_UTR 30.0%
TTCAGTGTTGTGAATCAAAT+GGG - chr7_4:57103249-57103268 Msa1165260:three_prime_UTR 30.0%
AATCATGGATCAAATGGATA+AGG - chr7_4:57103272-57103291 Msa1165260:three_prime_UTR 30.0%
ATGTTGTTCATACGATGAAA+TGG - chr7_4:57103411-57103430 Msa1165260:CDS 30.0%
TGTTGTTCATACGATGAAAT+GGG - chr7_4:57103412-57103431 Msa1165260:CDS 30.0%
GTGTTTGAAAATGAAGTTAG+TGG - chr7_4:57103940-57103959 Msa1165260:CDS 30.0%
AAAATGAAGTTAGTGGCATT+GGG - chr7_4:57103947-57103966 Msa1165260:CDS 30.0%
TTAAAATGTTAGAAGAAGGG+GGG - chr7_4:57104093-57104112 Msa1165260:intron 30.0%
ACTTAGTTCCAAATCTTGTT+AGG - chr7_4:57104268-57104287 Msa1165260:five_prime_UTR 30.0%
CTTAGTTCCAAATCTTGTTA+GGG - chr7_4:57104269-57104288 Msa1165260:five_prime_UTR 30.0%
TGTGAATCAAATGGGAATCA+TGG - chr7_4:57103257-57103276 Msa1165260:three_prime_UTR 35.0%
AATGGGAATCATGGATCAAA+TGG - chr7_4:57103266-57103285 Msa1165260:three_prime_UTR 35.0%
TTTCTTTGAGCTAACTAGCT+AGG - chr7_4:57103309-57103328 Msa1165260:three_prime_UTR 35.0%
AATCAGGTACAGAAAGAGAA+GGG - chr7_4:57103790-57103809 Msa1165260:intron 35.0%
TTGTAGCAAAGGACAAGAAA+GGG - chr7_4:57103817-57103836 Msa1165260:intron 35.0%
GAAAATGAAGTTAGTGGCAT+TGG - chr7_4:57103946-57103965 Msa1165260:CDS 35.0%
TGTTGCAGAAGAAACAAAGA+AGG - chr7_4:57103981-57104000 Msa1165260:CDS 35.0%
AAGGATTAAACTAGGTGAAG+AGG - chr7_4:57104000-57104019 Msa1165260:CDS 35.0%
TAAAATGTTAGAAGAAGGGG+GGG - chr7_4:57104094-57104113 Msa1165260:intron 35.0%
GAGAGAAAGAGAGAGAAAAT+TGG + chr7_4:57103118-57103137 None:intergenic 35.0%
TGTGAATCAAATGGGAATCA+TGG - chr7_4:57103257-57103276 Msa1165260:three_prime_UTR 35.0%
AATGGGAATCATGGATCAAA+TGG - chr7_4:57103266-57103285 Msa1165260:three_prime_UTR 35.0%
TTTCTTTGAGCTAACTAGCT+AGG - chr7_4:57103309-57103328 Msa1165260:three_prime_UTR 35.0%
AATCAGGTACAGAAAGAGAA+GGG - chr7_4:57103790-57103809 Msa1165260:intron 35.0%
TTGTAGCAAAGGACAAGAAA+GGG - chr7_4:57103817-57103836 Msa1165260:intron 35.0%
GAAAATGAAGTTAGTGGCAT+TGG - chr7_4:57103946-57103965 Msa1165260:CDS 35.0%
TGTTGCAGAAGAAACAAAGA+AGG - chr7_4:57103981-57104000 Msa1165260:CDS 35.0%
AAGGATTAAACTAGGTGAAG+AGG - chr7_4:57104000-57104019 Msa1165260:CDS 35.0%
TAAAATGTTAGAAGAAGGGG+GGG - chr7_4:57104094-57104113 Msa1165260:intron 35.0%
! TTTCGTTTCGGTGTGTGATT+CGG - chr7_4:57103170-57103189 Msa1165260:three_prime_UTR 40.0%
AAACCACCAAAACACCACTT+TGG - chr7_4:57103464-57103483 Msa1165260:CDS 40.0%
AATGATACCTTGGGTCCATT+TGG + chr7_4:57103499-57103518 None:intergenic 40.0%
GAATCAGGTACAGAAAGAGA+AGG - chr7_4:57103789-57103808 Msa1165260:intron 40.0%
ATCAGGTACAGAAAGAGAAG+GGG - chr7_4:57103791-57103810 Msa1165260:intron 40.0%
GTTGTAGCAAAGGACAAGAA+AGG - chr7_4:57103816-57103835 Msa1165260:intron 40.0%
CAAAGGACAAGAAAGGGAAA+GGG - chr7_4:57103823-57103842 Msa1165260:intron 40.0%
ACAAGAAAGGGAAAGGGAAA+GGG - chr7_4:57103829-57103848 Msa1165260:intron 40.0%
TCCAAATCTTGTTAGGGACT+AGG - chr7_4:57104275-57104294 Msa1165260:five_prime_UTR 40.0%
CCAAATCTTGTTAGGGACTA+GGG - chr7_4:57104276-57104295 Msa1165260:five_prime_UTR 40.0%
! TTTCGTTTCGGTGTGTGATT+CGG - chr7_4:57103170-57103189 Msa1165260:three_prime_UTR 40.0%
AAACCACCAAAACACCACTT+TGG - chr7_4:57103464-57103483 Msa1165260:CDS 40.0%
AATGATACCTTGGGTCCATT+TGG + chr7_4:57103499-57103518 None:intergenic 40.0%
GAATCAGGTACAGAAAGAGA+AGG - chr7_4:57103789-57103808 Msa1165260:intron 40.0%
ATCAGGTACAGAAAGAGAAG+GGG - chr7_4:57103791-57103810 Msa1165260:intron 40.0%
GTTGTAGCAAAGGACAAGAA+AGG - chr7_4:57103816-57103835 Msa1165260:intron 40.0%
CAAAGGACAAGAAAGGGAAA+GGG - chr7_4:57103823-57103842 Msa1165260:intron 40.0%
ACAAGAAAGGGAAAGGGAAA+GGG - chr7_4:57103829-57103848 Msa1165260:intron 40.0%
TCCAAATCTTGTTAGGGACT+AGG - chr7_4:57104275-57104294 Msa1165260:five_prime_UTR 40.0%
CCAAATCTTGTTAGGGACTA+GGG - chr7_4:57104276-57104295 Msa1165260:five_prime_UTR 40.0%
!! TCTCCAAAGTGGTGTTTTGG+TGG + chr7_4:57103470-57103489 None:intergenic 45.0%
! ACCAAAACACCACTTTGGAG+AGG - chr7_4:57103469-57103488 Msa1165260:CDS 45.0%
! TCCTCTCCAAAGTGGTGTTT+TGG + chr7_4:57103473-57103492 None:intergenic 45.0%
! AAAACACCACTTTGGAGAGG+AGG - chr7_4:57103472-57103491 Msa1165260:CDS 45.0%
AGAAGGGGTTGTTGTAGCAA+AGG - chr7_4:57103806-57103825 Msa1165260:intron 45.0%
GCAAAGGACAAGAAAGGGAA+AGG - chr7_4:57103822-57103841 Msa1165260:intron 45.0%
GACAAGAAAGGGAAAGGGAA+AGG - chr7_4:57103828-57103847 Msa1165260:intron 45.0%
AAAGGGAAAGGGAAAGGGAA+AGG - chr7_4:57103834-57103853 Msa1165260:intron 45.0%
AGGGAAAGGAAGAGAGAGAA+AGG - chr7_4:57103848-57103867 Msa1165260:intron 45.0%
! AGAGAGAAAGGTGCACAGAA+TGG - chr7_4:57103860-57103879 Msa1165260:intron 45.0%
! GAGAGAAAGGTGCACAGAAT+GGG - chr7_4:57103861-57103880 Msa1165260:intron 45.0%
! AAAGGTGCACAGAATGGGAA+TGG - chr7_4:57103866-57103885 Msa1165260:intron 45.0%
AAGGTGCACAGAATGGGAAT+GGG - chr7_4:57103867-57103886 Msa1165260:intron 45.0%
ACAAGCTGCTGTGTGTTTGA+TGG - chr7_4:57104024-57104043 Msa1165260:intron 45.0%
! TGTTTGATGGCACTCTCTTG+TGG - chr7_4:57104037-57104056 Msa1165260:intron 45.0%
CCCTAGTCCCTAACAAGATT+TGG + chr7_4:57104279-57104298 None:intergenic 45.0%
!! TCTCCAAAGTGGTGTTTTGG+TGG + chr7_4:57103470-57103489 None:intergenic 45.0%
! ACCAAAACACCACTTTGGAG+AGG - chr7_4:57103469-57103488 Msa1165260:CDS 45.0%
! TCCTCTCCAAAGTGGTGTTT+TGG + chr7_4:57103473-57103492 None:intergenic 45.0%
! AAAACACCACTTTGGAGAGG+AGG - chr7_4:57103472-57103491 Msa1165260:CDS 45.0%
AGAAGGGGTTGTTGTAGCAA+AGG - chr7_4:57103806-57103825 Msa1165260:intron 45.0%
GCAAAGGACAAGAAAGGGAA+AGG - chr7_4:57103822-57103841 Msa1165260:intron 45.0%
GACAAGAAAGGGAAAGGGAA+AGG - chr7_4:57103828-57103847 Msa1165260:intron 45.0%
AAAGGGAAAGGGAAAGGGAA+AGG - chr7_4:57103834-57103853 Msa1165260:intron 45.0%
AGGGAAAGGAAGAGAGAGAA+AGG - chr7_4:57103848-57103867 Msa1165260:intron 45.0%
! AGAGAGAAAGGTGCACAGAA+TGG - chr7_4:57103860-57103879 Msa1165260:intron 45.0%
! GAGAGAAAGGTGCACAGAAT+GGG - chr7_4:57103861-57103880 Msa1165260:intron 45.0%
! AAAGGTGCACAGAATGGGAA+TGG - chr7_4:57103866-57103885 Msa1165260:intron 45.0%
AAGGTGCACAGAATGGGAAT+GGG - chr7_4:57103867-57103886 Msa1165260:intron 45.0%
ACAAGCTGCTGTGTGTTTGA+TGG - chr7_4:57104024-57104043 Msa1165260:intron 45.0%
! TGTTTGATGGCACTCTCTTG+TGG - chr7_4:57104037-57104056 Msa1165260:intron 45.0%
CCCTAGTCCCTAACAAGATT+TGG + chr7_4:57104279-57104298 None:intergenic 45.0%
TTTGGTCCTCCTCTCCAAAG+TGG + chr7_4:57103481-57103500 None:intergenic 50.0%
! CTTTGGAGAGGAGGACCAAA+TGG - chr7_4:57103481-57103500 Msa1165260:CDS 50.0%
TAGACGTTGTGCAACGCTTG+TGG - chr7_4:57103767-57103786 Msa1165260:intron 50.0%
TGTGCAACGCTTGTGGAATC+AGG - chr7_4:57103774-57103793 Msa1165260:intron 50.0%
TGCACAGAATGGGAATGGGA+GGG - chr7_4:57103871-57103890 Msa1165260:intron 50.0%
TTGTTAGGGACTAGGGAGTG+AGG - chr7_4:57104283-57104302 Msa1165260:five_prime_UTR 50.0%
TTTGGTCCTCCTCTCCAAAG+TGG + chr7_4:57103481-57103500 None:intergenic 50.0%
! CTTTGGAGAGGAGGACCAAA+TGG - chr7_4:57103481-57103500 Msa1165260:CDS 50.0%
TAGACGTTGTGCAACGCTTG+TGG - chr7_4:57103767-57103786 Msa1165260:intron 50.0%
TGTGCAACGCTTGTGGAATC+AGG - chr7_4:57103774-57103793 Msa1165260:intron 50.0%
TGCACAGAATGGGAATGGGA+GGG - chr7_4:57103871-57103890 Msa1165260:intron 50.0%
TTGTTAGGGACTAGGGAGTG+AGG - chr7_4:57104283-57104302 Msa1165260:five_prime_UTR 50.0%
AGGAGGACCAAATGGACCCA+AGG - chr7_4:57103489-57103508 Msa1165260:CDS 55.0%
GTGCACAGAATGGGAATGGG+AGG - chr7_4:57103870-57103889 Msa1165260:intron 55.0%
GCACAGAATGGGAATGGGAG+GGG - chr7_4:57103872-57103891 Msa1165260:intron 55.0%
GGTGGTACTAGCAGTAGCAG+TGG - chr7_4:57103893-57103912 Msa1165260:intron 55.0%
TTAGGGACTAGGGAGTGAGG+TGG - chr7_4:57104286-57104305 Msa1165260:five_prime_UTR 55.0%
AGGAGGACCAAATGGACCCA+AGG - chr7_4:57103489-57103508 Msa1165260:CDS 55.0%
GTGCACAGAATGGGAATGGG+AGG - chr7_4:57103870-57103889 Msa1165260:intron 55.0%
GCACAGAATGGGAATGGGAG+GGG - chr7_4:57103872-57103891 Msa1165260:intron 55.0%
GGTGGTACTAGCAGTAGCAG+TGG - chr7_4:57103893-57103912 Msa1165260:intron 55.0%
TTAGGGACTAGGGAGTGAGG+TGG - chr7_4:57104286-57104305 Msa1165260:five_prime_UTR 55.0%
CAGAATGGGAATGGGAGGGG+TGG - chr7_4:57103875-57103894 Msa1165260:intron 60.0%
CAGAATGGGAATGGGAGGGG+TGG - chr7_4:57103875-57103894 Msa1165260:intron 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 57103118 57104321 57103118 ID=Msa1165260;Name=Msa1165260
chr7_4 mRNA 57103118 57104321 57103118 ID=Msa1165260-mRNA-1;Parent=Msa1165260;Name=Msa1165260-mRNA-1;_AED=0.00;_eAED=0.00;_QI=149|1|1|1|1|1|3|246|144
chr7_4 exon 57103118 57103669 57103118 ID=Msa1165260-mRNA-1:exon:17197;Parent=Msa1165260-mRNA-1
chr7_4 exon 57103929 57104030 57103929 ID=Msa1165260-mRNA-1:exon:17196;Parent=Msa1165260-mRNA-1
chr7_4 exon 57104146 57104321 57104146 ID=Msa1165260-mRNA-1:exon:17195;Parent=Msa1165260-mRNA-1
chr7_4 five_prime_UTR 57104173 57104321 57104173 ID=Msa1165260-mRNA-1:five_prime_utr;Parent=Msa1165260-mRNA-1
chr7_4 CDS 57104146 57104172 57104146 ID=Msa1165260-mRNA-1:cds;Parent=Msa1165260-mRNA-1
chr7_4 CDS 57103929 57104030 57103929 ID=Msa1165260-mRNA-1:cds;Parent=Msa1165260-mRNA-1
chr7_4 CDS 57103364 57103669 57103364 ID=Msa1165260-mRNA-1:cds;Parent=Msa1165260-mRNA-1
chr7_4 three_prime_UTR 57103118 57103363 57103118 ID=Msa1165260-mRNA-1:three_prime_utr;Parent=Msa1165260-mRNA-1
Gene Sequence

>Msa1165260

ATGGGAATCATGGATCAAATGGATAAGGAATGTTGTTCATACGATGAAATGGGTGTGATCAAGAAATTTTGTTCTGATTGCAAAACCACCAAAACACCACTTTGGAGAGGAGGACCAAATGGACCCAAGACGTTGTGCAACGCTTGTGGAATCAGGTACAGAAAGAGAAGGGGTTGTTGTAGCAAAGGACAAGAAAGGGAAAGGGAAAGGGAAAGGAAGAGAGAGAAAGGTGCACAGAATGGGAATGGGAGGGGTGGTACTAGCAGTAGCAGTGGCAGTGACAATGATGATTTTAGTGAGTGTTTGAAAATGAAGTTAGTGGCATTGGGTGAAGAGTTTTTGTTGCAGAAGAAACAAAGAAGGATTAAACTAGGTGAAGAGGAACAAGCTGCTGTGTGTTTGATGGCACTCTCTTGTGGCTTTGTTTTTGCTTGA
Protein sequence

>Msa1165260

MGIMDQMDKECCSYDEMGVIKKFCSDCKTTKTPLWRGGPNGPKTLCNACGIRYRKRRGCCSKGQERERERERKREKGAQNGNGRGGTSSSSGSDNDDFSECLKMKLVALGEEFLLQKKQRRIKLGEEEQAAVCLMALSCGFVFA*