AlfalfaGEDB | Msa1167430

M. sativa cultivar zhongmu-4 / Msa1167430

KEGG: K11276
GO: GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006355 GO:0006950 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009890 GO:0009892 GO:0010033 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0070013 GO:0080090 GO:0097159 GO:0097305 GO:1901363 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
PFAM: PF17800.4

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1167430	RHN48623.1	78.261	299	28	6	1	272	1	289	5.41e-129	377

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1167430	sp\|Q6V9I6\|HDT1_SOLCH	42.403	283	135	8	1	271	1	267	2.64e-48	164

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1167430	A0A396H772	78.261	299	28	6	1	272	1	289	2.58e-129	377

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TFs/TRs:

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Gene ID	Type	Classification
Msa1167430	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1080030	Msa1167430	0.945590	2.298365e-104	-8.615850e-47
Msa1090640	Msa1167430	0.982108	2.990949e-154	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1167430	MtrunA17_Chr7g0265761	78.261	299	28	6	1	272	1	289	4.98e-133	377
Msa1167430	MtrunA17_Chr2g0320884	40.864	301	143	8	1	272	35	329	2.36e-42	147
Msa1167430	MtrunA17_Chr4g0027181	38.075	239	134	7	1	230	5	238	6.69e-28	108
Msa1167430	MtrunA17_Chr4g0027191	50.000	96	42	2	2	93	6	99	1.03e-22	95.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1167430	AT5G03740.1	40.273	293	152	7	1	271	1	292	9.33e-48	160
Msa1167430	AT3G44750.2	36.996	273	137	6	1	271	1	240	7.49e-39	136
Msa1167430	AT3G44750.1	35.531	273	144	6	1	271	1	243	1.28e-36	130
Msa1167430	AT5G22650.2	34.722	288	132	9	1	247	1	273	4.36e-26	104
Msa1167430	AT5G22650.1	34.602	289	132	9	1	247	1	274	5.73e-26	104
Msa1167430	AT2G27840.4	47.368	95	46	3	1	93	1	93	8.79e-21	86.7
Msa1167430	AT2G27840.3	47.368	95	46	3	1	93	1	93	2.12e-20	87.0
Msa1167430	AT2G27840.1	47.368	95	46	3	1	93	1	93	3.39e-20	84.7
Msa1167430	AT2G27840.2	46.575	73	35	3	23	93	1	71	5.43e-11	60.8

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Find 83 sgRNAs with CRISPR-Local

Find 290 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATGGTGAACCACACA+AGG	0.630125	7_4:-59905234	Msa1167430:CDS
AACACCTGTACTTGTGAAAA+AGG	0.297237	7_4:-59903366	Msa1167430:CDS
AACTCCAATCACCTGTTACA+GGG	0.611246	7_4:+59902675	None:intergenic
AACTTCACATACAGGGATAC+CGG	0.456270	7_4:+59905036	None:intergenic
AAGAACTAACCAGTCTTCTC+TGG	0.427252	7_4:+59903329	None:intergenic
AAGCATATACCGATGAAATG+TGG	0.617686	7_4:+59905186	None:intergenic
AAGCGATGAAGATTCTGACA+AGG	0.637276	7_4:-59903514	Msa1167430:CDS
AATCAGAAGGATGCTAGTAC+TGG	0.537632	7_4:-59904451	Msa1167430:CDS
AATTTGAGCTATCCCATAAC+TGG	0.378730	7_4:-59904933	Msa1167430:CDS
ACAGCTGCAATCCCTGTAAC+AGG	0.418416	7_4:-59902686	Msa1167430:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGTAAAGTGATAGAAAGG+AGG	+	chr7_4:59902411-59902430	None:intergenic	30.0%
AGAATCGCATATAAAACGAT+AGG	-	chr7_4:59902515-59902534	Msa1167430:three_prime_UTR	30.0%
TTGTTAAATAGCAACTACAG+CGG	+	chr7_4:59902942-59902961	None:intergenic	30.0%
AACAAAACAAAAAAACGTCG+AGG	+	chr7_4:59903001-59903020	None:intergenic	30.0%
GTTCATTTCAGAGATTAGAT+TGG	+	chr7_4:59903109-59903128	None:intergenic	30.0%
CATTGTCTTAGACCAATAAA+AGG	-	chr7_4:59903270-59903289	Msa1167430:intron	30.0%
GTTACAAAATCAGAGACATA+AGG	+	chr7_4:59903345-59903364	None:intergenic	30.0%
TATAAAACTCCTTTCATGTG+GGG	+	chr7_4:59903637-59903656	None:intergenic	30.0%
GATATAAAACTCCTTTCATG+TGG	+	chr7_4:59903639-59903658	None:intergenic	30.0%
TCACTGGGTTGTTAATAATA+TGG	-	chr7_4:59903657-59903676	Msa1167430:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	59902239	59906842	59902239	ID=Msa1167430;Name=Msa1167430
chr7_4	mRNA	59902239	59906842	59902239	ID=Msa1167430-mRNA-1;Parent=Msa1167430;Name=Msa1167430-mRNA-1;_AED=0.23;_eAED=0.23;_QI=141\|0.87\|0.88\|1\|0.87\|0.88\|9\|296\|272
chr7_4	exon	59902239	59902595	59902239	ID=Msa1167430-mRNA-1:exon:18653;Parent=Msa1167430-mRNA-1
chr7_4	exon	59902687	59902807	59902687	ID=Msa1167430-mRNA-1:exon:18652;Parent=Msa1167430-mRNA-1
chr7_4	exon	59903339	59903432	59903339	ID=Msa1167430-mRNA-1:exon:18651;Parent=Msa1167430-mRNA-1
chr7_4	exon	59903500	59903577	59903500	ID=Msa1167430-mRNA-1:exon:18650;Parent=Msa1167430-mRNA-1
chr7_4	exon	59904381	59904517	59904381	ID=Msa1167430-mRNA-1:exon:18649;Parent=Msa1167430-mRNA-1
chr7_4	exon	59904605	59904643	59904605	ID=Msa1167430-mRNA-1:exon:18648;Parent=Msa1167430-mRNA-1
chr7_4	exon	59904893	59905097	59904893	ID=Msa1167430-mRNA-1:exon:18647;Parent=Msa1167430-mRNA-1
chr7_4	exon	59905196	59905266	59905196	ID=Msa1167430-mRNA-1:exon:18646;Parent=Msa1167430-mRNA-1
chr7_4	exon	59906689	59906842	59906689	ID=Msa1167430-mRNA-1:exon:18645;Parent=Msa1167430-mRNA-1
chr7_4	five_prime_UTR	59906702	59906842	59906702	ID=Msa1167430-mRNA-1:five_prime_utr;Parent=Msa1167430-mRNA-1
chr7_4	CDS	59906689	59906701	59906689	ID=Msa1167430-mRNA-1:cds;Parent=Msa1167430-mRNA-1
chr7_4	CDS	59905196	59905266	59905196	ID=Msa1167430-mRNA-1:cds;Parent=Msa1167430-mRNA-1
chr7_4	CDS	59904893	59905097	59904893	ID=Msa1167430-mRNA-1:cds;Parent=Msa1167430-mRNA-1
chr7_4	CDS	59904605	59904643	59904605	ID=Msa1167430-mRNA-1:cds;Parent=Msa1167430-mRNA-1
chr7_4	CDS	59904381	59904517	59904381	ID=Msa1167430-mRNA-1:cds;Parent=Msa1167430-mRNA-1
chr7_4	CDS	59903500	59903577	59903500	ID=Msa1167430-mRNA-1:cds;Parent=Msa1167430-mRNA-1
chr7_4	CDS	59903339	59903432	59903339	ID=Msa1167430-mRNA-1:cds;Parent=Msa1167430-mRNA-1
chr7_4	CDS	59902687	59902807	59902687	ID=Msa1167430-mRNA-1:cds;Parent=Msa1167430-mRNA-1
chr7_4	CDS	59902535	59902595	59902535	ID=Msa1167430-mRNA-1:cds;Parent=Msa1167430-mRNA-1
chr7_4	three_prime_UTR	59902239	59902534	59902239	ID=Msa1167430-mRNA-1:three_prime_utr;Parent=Msa1167430-mRNA-1

Gene Sequence

>Msa1167430

ATGGAGTTTTGGGGTGTCGAGGTAAAACATGGTGAACCACACAAGGTTGTTCCAGAAAATAACCAGCTTATCCACATTTCATCGGCATGTTTGGGGGAGGTCAGCAAGGATAAAGGGAGTGACCCGGTATCCCTGTATGTGAAGTTTGGTGATCACAAGCTTCAAATTGGAACACTTTCTTCAGAAAAATTTCCTCAGACATCTTTTGATTTGGTTTTTGGGAAGGAATTTGAGCTATCCCATAACTGGAAATATGGGAGCGTCTTCCTTGCTGGATTTTTAATCGAATATACTGATTCTGAAGAGGAAGATATTATACTTGCTAATGGGAAACCTGCCAAACCCGGTGTAAATGCATCAAAACAGAAAAAGAATCAGAAGGATGCTAGTACTGGCGATAGTTCAGATTCATCTGATGCTAGTACTGGCGATAGTTCAGATTCATCTGATGCTAGTGAAGATGAGCCCATGGCGAATGGTCAAAATAAAAGTAGTAGTGAAGATGAAAGCGATGAAGATTCTGACAAGGAGACTCCGAAGAAGACTGAAGGGAGCAACAAGAGGAGGGATGCTGAATCTTCCAAGGAAACACCTGTACTTGTGAAAAAGGCAAAATTTGTTACTCCAGAGAAGACTGGTGGCAGGAGTAGTGTCCCTTACCCTAAGCAGGCTGGTAAATCTGCTGCAAATAACAAGCAGCCAGAGAAGCAGGAGACTCCAAAGTCTAGTGGAGATTACAGCTGCAATCCCTGTAACAGGTCTTTTAAGACTGAAGATGCCTTAGGTTCTCATAATAAGGCCAAGCACAGTGCCAAGTGA

Protein sequence

>Msa1167430

MEFWGVEVKHGEPHKVVPENNQLIHISSACLGEVSKDKGSDPVSLYVKFGDHKLQIGTLSSEKFPQTSFDLVFGKEFELSHNWKYGSVFLAGFLIEYTDSEEEDIILANGKPAKPGVNASKQKKNQKDASTGDSSDSSDASTGDSSDSSDASEDEPMANGQNKSSSEDESDEDSDKETPKKTEGSNKRRDAESSKETPVLVKKAKFVTPEKTGGRSSVPYPKQAGKSAANNKQPEKQETPKSSGDYSCNPCNRSFKTEDALGSHNKAKHSAK*