AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1173060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1173060 MtrunA17_Chr1g0152431 88.889 207 11 2 1 207 1 195 3.11e-132 369
Msa1173060 MtrunA17_Chr4g0059371 58.824 187 64 3 2 188 3 176 2.05e-75 225
Msa1173060 MtrunA17_Chr3g0113051 51.323 189 76 4 1 187 1 175 6.96e-58 181
Msa1173060 MtrunA17_Chr8g0349391 45.128 195 88 5 1 194 1 177 5.85e-50 161
Msa1173060 MtrunA17_Chr7g0247111 44.385 187 88 4 1 187 1 171 1.18e-48 158
Msa1173060 MtrunA17_Chr8g0386501 46.561 189 77 6 1 187 1 167 9.22e-48 155
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1173060 AT1G10200.1 74.872 195 37 2 1 195 1 183 1.81e-102 294
Msa1173060 AT1G10200.2 80.800 125 24 0 71 195 17 141 2.60e-71 213
Msa1173060 AT2G39900.1 50.794 189 76 4 1 187 1 174 5.78e-57 179
Msa1173060 AT3G55770.3 48.168 191 77 4 1 187 1 173 7.51e-55 174
Msa1173060 AT3G55770.3 43.678 87 49 0 115 201 3 89 8.73e-16 72.8
Msa1173060 AT3G55770.5 48.168 191 77 4 1 187 1 173 7.51e-55 174
Msa1173060 AT3G55770.5 43.678 87 49 0 115 201 3 89 8.73e-16 72.8
Msa1173060 AT3G55770.2 48.168 191 77 4 1 187 1 173 7.51e-55 174
Msa1173060 AT3G55770.2 43.678 87 49 0 115 201 3 89 8.73e-16 72.8
Msa1173060 AT3G55770.1 48.168 191 77 4 1 187 1 173 7.51e-55 174
Msa1173060 AT3G55770.1 43.678 87 49 0 115 201 3 89 8.73e-16 72.8
Msa1173060 AT2G45800.1 43.939 198 92 4 1 195 1 182 4.88e-49 159
Msa1173060 AT1G01780.1 41.624 197 95 5 1 195 1 179 7.30e-48 156
Msa1173060 AT3G61230.1 42.157 204 99 5 2 202 3 190 8.28e-48 156
Msa1173060 AT3G55770.7 40.889 225 77 5 1 187 1 207 2.62e-47 155
Msa1173060 AT3G55770.7 43.678 87 49 0 115 201 3 89 1.95e-15 72.8
Msa1173060 AT2G45800.2 44.531 128 64 2 71 195 28 151 3.35e-30 110
Msa1173060 AT3G55770.4 40.288 139 61 4 1 135 1 121 7.87e-28 102
Msa1173060 AT3G55770.4 43.678 87 49 0 115 201 3 89 6.41e-17 74.3
Msa1173060 AT3G55770.6 39.437 142 64 4 1 138 1 124 6.50e-26 97.8
Msa1173060 AT3G55770.6 43.678 87 49 0 115 201 3 89 9.76e-17 73.9

Find 63 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTATCTATATTTCTTTC+TGG 0.117591 8_1:-3259049 None:intergenic
TTCTTTCATCATTTGGCTTA+TGG 0.156505 8_1:+3258743 Msa1173060:CDS
CTGGACTCTGATGCATCTTT+TGG 0.240018 8_1:+3259978 Msa1173060:three_prime_UTR
TCCCTCATGGGCTATGTAAT+TGG 0.293058 8_1:-3259616 None:intergenic
AATGAACTTCTTTCATCATT+TGG 0.298410 8_1:+3258736 Msa1173060:CDS
AGCATTTGAAACAACTCTTA+TGG 0.319973 8_1:-3259566 None:intergenic
ATTGAGCTGAAAATAATTGA+TGG 0.336079 8_1:-3260045 None:intergenic
TTTATAAAGGTTACAGTGAA+TGG 0.352930 8_1:+3259534 Msa1173060:intron
TTTGCTTGATCAGTTGAATA+TGG 0.353364 8_1:-3259659 None:intergenic
CTCCATACCGGAGTTACATT+TGG 0.371704 8_1:-3259931 None:intergenic
GTCACTGACAATATAATTGT+TGG 0.386890 8_1:+3258133 Msa1173060:five_prime_UTR
GTTCATTAACATGAAAATTA+CGG 0.392058 8_1:-3258720 None:intergenic
ACAAATATTGAATACCATAA+TGG 0.395661 8_1:-3259819 None:intergenic
TAAAAGAGAAAAGTATAGTT+AGG 0.405318 8_1:+3258085 None:intergenic
CTTGAGGCTTTAGCTGCTGC+AGG 0.425078 8_1:-3259174 None:intergenic
TATTCAACTGATCAAGCAAA+AGG 0.432443 8_1:+3259662 Msa1173060:CDS
GCTAAAGCCTCAAGTATGTT+TGG 0.441559 8_1:+3259183 Msa1173060:CDS
GACCAACTGTTCAAAAGAAC+TGG 0.444337 8_1:+3258887 Msa1173060:CDS
ACACTAATTACCTTCTCTGT+TGG 0.465114 8_1:-3259249 None:intergenic
GTTGGTGAGGTAAGAAGATA+AGG 0.471222 8_1:+3258266 Msa1173060:five_prime_UTR
CCGGTATGGAGTTCGTTACA+TGG 0.471286 8_1:+3259943 Msa1173060:three_prime_UTR
GGAAATTTGAGCCAGCTTGA+AGG 0.475377 8_1:+3259684 Msa1173060:CDS
GGAACAAGGGACAAATGTTC+TGG 0.477611 8_1:+3259207 Msa1173060:CDS
ACTTGTAGTTGTGGGTGAAC+AGG 0.478525 8_1:+3260078 Msa1173060:three_prime_UTR
TCTCATGGTCACCTTCAAGC+TGG 0.480325 8_1:-3259695 None:intergenic
AAGCGTATTAATAGAGTTCG+TGG 0.482813 8_1:+3260006 Msa1173060:three_prime_UTR
CAAGTATGTTTGGTGGAACA+AGG 0.495604 8_1:+3259193 Msa1173060:CDS
GGCACTGGTAAATGTAAGAA+AGG 0.497607 8_1:-3260141 None:intergenic
TTGAAACAACTCTTATGGTA+AGG 0.498185 8_1:-3259561 None:intergenic
AACAGGTTGAAAATTAGAAT+AGG 0.503037 8_1:+3260095 Msa1173060:three_prime_UTR
AACATAGCAACTTGTAGTTG+TGG 0.510923 8_1:+3260069 Msa1173060:three_prime_UTR
AGAAAGAAATATAGATAATG+AGG 0.518674 8_1:+3259051 Msa1173060:CDS
CCTTGAGGGTTCCTTTGCAG+TGG 0.520760 8_1:-3258409 None:intergenic
TTTCATCATTTGGCTTATGG+AGG 0.523794 8_1:+3258746 Msa1173060:CDS
GAGAGAGACATAGACCTTGA+GGG 0.525903 8_1:-3258423 None:intergenic
TTCATAGACTTGTTGCAAGT+TGG 0.534172 8_1:+3258248 Msa1173060:five_prime_UTR
ACATAGCAACTTGTAGTTGT+GGG 0.534197 8_1:+3260070 Msa1173060:three_prime_UTR
GACTAATAGTACACCCTCCA+TGG 0.536605 8_1:-3259590 None:intergenic
AACCAAATGTAACTCCGGTA+TGG 0.537455 8_1:+3259929 Msa1173060:three_prime_UTR
AGGAACAACTCAGAAGTGTA+TGG 0.560932 8_1:+3258307 Msa1173060:CDS
ATTCAACTGATCAAGCAAAA+GGG 0.562604 8_1:+3259663 Msa1173060:CDS
AGTTGTTTCAAATGCTGCCA+TGG 0.564010 8_1:+3259573 Msa1173060:CDS
GACATCTGAAACAAGCTTTG+TGG 0.565667 8_1:-3258385 None:intergenic
AATATTTGTTTGCAAGTGTG+CGG 0.578719 8_1:+3259833 Msa1173060:three_prime_UTR
TTGAACAGTTGGTCGAAGTG+TGG 0.580292 8_1:-3258878 None:intergenic
AGACTTGTTGCAAGTTGGTG+AGG 0.582330 8_1:+3258253 Msa1173060:five_prime_UTR
TTCAGATGTCACCACTGCAA+AGG 0.582786 8_1:+3258398 Msa1173060:CDS
GCTTGATCAGTTGAATATGG+TGG 0.582930 8_1:-3259656 None:intergenic
CCATGTAACGAACTCCATAC+CGG 0.586749 8_1:-3259943 None:intergenic
AAAGCCTCAAGTATGTTTGG+TGG 0.587944 8_1:+3259186 Msa1173060:CDS
ACGGAAACCAAATGTAACTC+CGG 0.588072 8_1:+3259924 Msa1173060:three_prime_UTR
TCCAATTACATAGCCCATGA+GGG 0.590710 8_1:+3259615 Msa1173060:CDS
ATGTGGCATAAAGAAAATTG+TGG 0.592279 8_1:-3258177 None:intergenic
AATAAAGTGGAGGAGAAATG+TGG 0.592781 8_1:-3258194 None:intergenic
GTTTCAAATGCTGCCATGGA+GGG 0.597804 8_1:+3259577 Msa1173060:CDS
TGTTCCACCAAACATACTTG+AGG 0.606764 8_1:-3259190 None:intergenic
AACAGTGTATCCAACAGAGA+AGG 0.613878 8_1:+3259239 Msa1173060:CDS
TTCCAATTACATAGCCCATG+AGG 0.614856 8_1:+3259614 Msa1173060:CDS
TCATGGGCTATGTAATTGGA+AGG 0.623562 8_1:-3259612 None:intergenic
AAGTATGTTTGGTGGAACAA+GGG 0.641097 8_1:+3259194 Msa1173060:CDS
CCACTGCAAAGGAACCCTCA+AGG 0.664342 8_1:+3258409 Msa1173060:CDS
TGTTTCAAATGCTGCCATGG+AGG 0.668776 8_1:+3259576 Msa1173060:CDS
CATTAACATGAAAATTACGG+TGG 0.679221 8_1:-3258717 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTCAAAAATAATTTTGTT+CGG - chr8_1:3259399-3259418 None:intergenic 15.0%
!!! TCAATAATTTGCATTTATAA+AGG + chr8_1:3259521-3259540 Msa1173060:intron 15.0%
!!! GTGTTTTATTTTGATTTTAA+CGG + chr8_1:3259905-3259924 Msa1173060:three_prime_UTR 15.0%
!!! AACTCAAAAATAATTTTGTT+CGG - chr8_1:3259399-3259418 None:intergenic 15.0%
!!! TCAATAATTTGCATTTATAA+AGG + chr8_1:3259521-3259540 Msa1173060:intron 15.0%
!!! GTGTTTTATTTTGATTTTAA+CGG + chr8_1:3259905-3259924 Msa1173060:three_prime_UTR 15.0%
!! TTGTAGCTTCAATAAAATTA+CGG - chr8_1:3258587-3258606 None:intergenic 20.0%
!! GTTCATTAACATGAAAATTA+CGG - chr8_1:3258723-3258742 None:intergenic 20.0%
!! TCATTATCTATATTTCTTTC+TGG - chr8_1:3259052-3259071 None:intergenic 20.0%
!! AGAAAGAAATATAGATAATG+AGG + chr8_1:3259051-3259070 Msa1173060:CDS 20.0%
!! ACAAATATTGAATACCATAA+TGG - chr8_1:3259822-3259841 None:intergenic 20.0%
!! TTGTAGCTTCAATAAAATTA+CGG - chr8_1:3258587-3258606 None:intergenic 20.0%
!! GTTCATTAACATGAAAATTA+CGG - chr8_1:3258723-3258742 None:intergenic 20.0%
!! TCATTATCTATATTTCTTTC+TGG - chr8_1:3259052-3259071 None:intergenic 20.0%
!! AGAAAGAAATATAGATAATG+AGG + chr8_1:3259051-3259070 Msa1173060:CDS 20.0%
!! ACAAATATTGAATACCATAA+TGG - chr8_1:3259822-3259841 None:intergenic 20.0%
! GAACAAAAAAGATTATGAAG+AGG - chr8_1:3258231-3258250 None:intergenic 25.0%
! AAGAAGATAAGGAAAAAGAA+TGG + chr8_1:3258277-3258296 Msa1173060:exon 25.0%
! CATAGCTTAAGTAAAAACTA+TGG + chr8_1:3258609-3258628 Msa1173060:intron 25.0%
! GAAAATTGTTACTGCATATT+TGG - chr8_1:3258662-3258681 None:intergenic 25.0%
! AATGAACTTCTTTCATCATT+TGG + chr8_1:3258736-3258755 Msa1173060:CDS 25.0%
!!! TACTTTTGTTTTGCATACAT+AGG + chr8_1:3259007-3259026 Msa1173060:intron 25.0%
!!! ACTTTTGTTTTGCATACATA+GGG + chr8_1:3259008-3259027 Msa1173060:intron 25.0%
! AGGTAATGAATTCTAAATCT+AGG + chr8_1:3259071-3259090 Msa1173060:intron 25.0%
! AAACAGCTCAAAAAATCATA+TGG - chr8_1:3259144-3259163 None:intergenic 25.0%
! AGGTAATTAGTGTATATGTT+TGG + chr8_1:3259259-3259278 Msa1173060:intron 25.0%
!! AGTGTATATGTTTGGAATTA+CGG + chr8_1:3259267-3259286 Msa1173060:intron 25.0%
!!! TTTTGAGTTTGTGATTAGTT+TGG + chr8_1:3259410-3259429 Msa1173060:intron 25.0%
!!! AAGTTTTGTATTCAACATCA+GGG - chr8_1:3259483-3259502 None:intergenic 25.0%
!!! TAAGTTTTGTATTCAACATC+AGG - chr8_1:3259484-3259503 None:intergenic 25.0%
! TTTATAAAGGTTACAGTGAA+TGG + chr8_1:3259534-3259553 Msa1173060:intron 25.0%
! ATTGAGCTGAAAATAATTGA+TGG - chr8_1:3260048-3260067 None:intergenic 25.0%
!! AACAGGTTGAAAATTAGAAT+AGG + chr8_1:3260095-3260114 Msa1173060:three_prime_UTR 25.0%
!!! GCTTTATTTTCAATTTCTGA+TGG - chr8_1:3260165-3260184 None:intergenic 25.0%
! GAACAAAAAAGATTATGAAG+AGG - chr8_1:3258231-3258250 None:intergenic 25.0%
! AAGAAGATAAGGAAAAAGAA+TGG + chr8_1:3258277-3258296 Msa1173060:exon 25.0%
! CATAGCTTAAGTAAAAACTA+TGG + chr8_1:3258609-3258628 Msa1173060:intron 25.0%
! GAAAATTGTTACTGCATATT+TGG - chr8_1:3258662-3258681 None:intergenic 25.0%
! AATGAACTTCTTTCATCATT+TGG + chr8_1:3258736-3258755 Msa1173060:CDS 25.0%
!!! TACTTTTGTTTTGCATACAT+AGG + chr8_1:3259007-3259026 Msa1173060:intron 25.0%
!!! ACTTTTGTTTTGCATACATA+GGG + chr8_1:3259008-3259027 Msa1173060:intron 25.0%
! AGGTAATGAATTCTAAATCT+AGG + chr8_1:3259071-3259090 Msa1173060:intron 25.0%
! AAACAGCTCAAAAAATCATA+TGG - chr8_1:3259144-3259163 None:intergenic 25.0%
! AGGTAATTAGTGTATATGTT+TGG + chr8_1:3259259-3259278 Msa1173060:intron 25.0%
!! AGTGTATATGTTTGGAATTA+CGG + chr8_1:3259267-3259286 Msa1173060:intron 25.0%
!!! TTTTGAGTTTGTGATTAGTT+TGG + chr8_1:3259410-3259429 Msa1173060:intron 25.0%
!!! AAGTTTTGTATTCAACATCA+GGG - chr8_1:3259483-3259502 None:intergenic 25.0%
!!! TAAGTTTTGTATTCAACATC+AGG - chr8_1:3259484-3259503 None:intergenic 25.0%
! TTTATAAAGGTTACAGTGAA+TGG + chr8_1:3259534-3259553 Msa1173060:intron 25.0%
! ATTGAGCTGAAAATAATTGA+TGG - chr8_1:3260048-3260067 None:intergenic 25.0%
!! AACAGGTTGAAAATTAGAAT+AGG + chr8_1:3260095-3260114 Msa1173060:three_prime_UTR 25.0%
!!! GCTTTATTTTCAATTTCTGA+TGG - chr8_1:3260165-3260184 None:intergenic 25.0%
GTCACTGACAATATAATTGT+TGG + chr8_1:3258133-3258152 Msa1173060:five_prime_UTR 30.0%
ATGTGGCATAAAGAAAATTG+TGG - chr8_1:3258180-3258199 None:intergenic 30.0%
GAGGAATAAAAATAAAGTGG+AGG - chr8_1:3258207-3258226 None:intergenic 30.0%
GGAGAGGAATAAAAATAAAG+TGG - chr8_1:3258210-3258229 None:intergenic 30.0%
AAAAAGATTATGAAGAGGAG+AGG - chr8_1:3258226-3258245 None:intergenic 30.0%
! ACCTCTTAGACATTTCTTTT+TGG + chr8_1:3258449-3258468 Msa1173060:intron 30.0%
ACCAAAAAGAAATGTCTAAG+AGG - chr8_1:3258453-3258472 None:intergenic 30.0%
TTACTGCATATTTGGATTCA+CGG - chr8_1:3258654-3258673 None:intergenic 30.0%
TGAAAATGCTTATGAACAAC+TGG - chr8_1:3258690-3258709 None:intergenic 30.0%
CATTAACATGAAAATTACGG+TGG - chr8_1:3258720-3258739 None:intergenic 30.0%
! TTCTTTCATCATTTGGCTTA+TGG + chr8_1:3258743-3258762 Msa1173060:CDS 30.0%
! AAGCAACTACAATTCTTTTG+AGG + chr8_1:3258838-3258857 Msa1173060:intron 30.0%
! AGCAACTACAATTCTTTTGA+GGG + chr8_1:3258839-3258858 Msa1173060:intron 30.0%
!!! TACCTTCAAAGCTTTTTTCA+AGG - chr8_1:3258916-3258935 None:intergenic 30.0%
!! AAAAGCTTTGAAGGTAACAA+AGG + chr8_1:3258920-3258939 Msa1173060:intron 30.0%
!!! CTTTCTGGTTTGACAATTTT+TGG - chr8_1:3259037-3259056 None:intergenic 30.0%
!!! TGTAGCCTTTTCTAAAATCA+TGG + chr8_1:3259340-3259359 Msa1173060:intron 30.0%
TTGAAACAACTCTTATGGTA+AGG - chr8_1:3259564-3259583 None:intergenic 30.0%
! AGCATTTGAAACAACTCTTA+TGG - chr8_1:3259569-3259588 None:intergenic 30.0%
TTTGCTTGATCAGTTGAATA+TGG - chr8_1:3259662-3259681 None:intergenic 30.0%
TATTCAACTGATCAAGCAAA+AGG + chr8_1:3259662-3259681 Msa1173060:CDS 30.0%
ATTCAACTGATCAAGCAAAA+GGG + chr8_1:3259663-3259682 Msa1173060:CDS 30.0%
AAAAATGCTGGGAAAATCAA+TGG + chr8_1:3259717-3259736 Msa1173060:CDS 30.0%
AATATTTGTTTGCAAGTGTG+CGG + chr8_1:3259833-3259852 Msa1173060:three_prime_UTR 30.0%
!!! AGTTCGTTACATGGTTTTAT+TGG + chr8_1:3259952-3259971 Msa1173060:three_prime_UTR 30.0%
GTCACTGACAATATAATTGT+TGG + chr8_1:3258133-3258152 Msa1173060:five_prime_UTR 30.0%
ATGTGGCATAAAGAAAATTG+TGG - chr8_1:3258180-3258199 None:intergenic 30.0%
GAGGAATAAAAATAAAGTGG+AGG - chr8_1:3258207-3258226 None:intergenic 30.0%
GGAGAGGAATAAAAATAAAG+TGG - chr8_1:3258210-3258229 None:intergenic 30.0%
AAAAAGATTATGAAGAGGAG+AGG - chr8_1:3258226-3258245 None:intergenic 30.0%
! ACCTCTTAGACATTTCTTTT+TGG + chr8_1:3258449-3258468 Msa1173060:intron 30.0%
ACCAAAAAGAAATGTCTAAG+AGG - chr8_1:3258453-3258472 None:intergenic 30.0%
TTACTGCATATTTGGATTCA+CGG - chr8_1:3258654-3258673 None:intergenic 30.0%
TGAAAATGCTTATGAACAAC+TGG - chr8_1:3258690-3258709 None:intergenic 30.0%
CATTAACATGAAAATTACGG+TGG - chr8_1:3258720-3258739 None:intergenic 30.0%
! TTCTTTCATCATTTGGCTTA+TGG + chr8_1:3258743-3258762 Msa1173060:CDS 30.0%
! AAGCAACTACAATTCTTTTG+AGG + chr8_1:3258838-3258857 Msa1173060:intron 30.0%
! AGCAACTACAATTCTTTTGA+GGG + chr8_1:3258839-3258858 Msa1173060:intron 30.0%
!!! TACCTTCAAAGCTTTTTTCA+AGG - chr8_1:3258916-3258935 None:intergenic 30.0%
!! AAAAGCTTTGAAGGTAACAA+AGG + chr8_1:3258920-3258939 Msa1173060:intron 30.0%
!!! CTTTCTGGTTTGACAATTTT+TGG - chr8_1:3259037-3259056 None:intergenic 30.0%
!!! TGTAGCCTTTTCTAAAATCA+TGG + chr8_1:3259340-3259359 Msa1173060:intron 30.0%
TTGAAACAACTCTTATGGTA+AGG - chr8_1:3259564-3259583 None:intergenic 30.0%
! AGCATTTGAAACAACTCTTA+TGG - chr8_1:3259569-3259588 None:intergenic 30.0%
TTTGCTTGATCAGTTGAATA+TGG - chr8_1:3259662-3259681 None:intergenic 30.0%
TATTCAACTGATCAAGCAAA+AGG + chr8_1:3259662-3259681 Msa1173060:CDS 30.0%
ATTCAACTGATCAAGCAAAA+GGG + chr8_1:3259663-3259682 Msa1173060:CDS 30.0%
AAAAATGCTGGGAAAATCAA+TGG + chr8_1:3259717-3259736 Msa1173060:CDS 30.0%
AATATTTGTTTGCAAGTGTG+CGG + chr8_1:3259833-3259852 Msa1173060:three_prime_UTR 30.0%
!!! AGTTCGTTACATGGTTTTAT+TGG + chr8_1:3259952-3259971 Msa1173060:three_prime_UTR 30.0%
AATAAAGTGGAGGAGAAATG+TGG - chr8_1:3258197-3258216 None:intergenic 35.0%
!! TTCATAGACTTGTTGCAAGT+TGG + chr8_1:3258248-3258267 Msa1173060:five_prime_UTR 35.0%
! CTAGTTTAGCCTTCTAGTAT+TGG + chr8_1:3258487-3258506 Msa1173060:intron 35.0%
CTAACAGATCCAATACTAGA+AGG - chr8_1:3258499-3258518 None:intergenic 35.0%
! TTTCATCATTTGGCTTATGG+AGG + chr8_1:3258746-3258765 Msa1173060:CDS 35.0%
! TACCAGTTCTTTTGAACAGT+TGG - chr8_1:3258892-3258911 None:intergenic 35.0%
! AGCCTTGAAAAAAGCTTTGA+AGG + chr8_1:3258911-3258930 Msa1173060:CDS 35.0%
! AAGTATGTTTGGTGGAACAA+GGG + chr8_1:3259194-3259213 Msa1173060:CDS 35.0%
ACACTAATTACCTTCTCTGT+TGG - chr8_1:3259252-3259271 None:intergenic 35.0%
!! CGGCTAGTTTAACAATATCA+CGG + chr8_1:3259287-3259306 Msa1173060:intron 35.0%
!!! AGCCTTTTCTAAAATCATGG+TGG + chr8_1:3259343-3259362 Msa1173060:intron 35.0%
!! GTCCACCATGATTTTAGAAA+AGG - chr8_1:3259348-3259367 None:intergenic 35.0%
! TTTTCCCAGCATTTTTCTCA+TGG - chr8_1:3259713-3259732 None:intergenic 35.0%
!! CATGTTTTTGCTTGCCATTA+TGG + chr8_1:3259805-3259824 Msa1173060:three_prime_UTR 35.0%
AAGCGTATTAATAGAGTTCG+TGG + chr8_1:3260006-3260025 Msa1173060:three_prime_UTR 35.0%
AACATAGCAACTTGTAGTTG+TGG + chr8_1:3260069-3260088 Msa1173060:three_prime_UTR 35.0%
ACATAGCAACTTGTAGTTGT+GGG + chr8_1:3260070-3260089 Msa1173060:three_prime_UTR 35.0%
! TTTTCAATTTCTGATGGCAC+TGG - chr8_1:3260159-3260178 None:intergenic 35.0%
AATAAAGTGGAGGAGAAATG+TGG - chr8_1:3258197-3258216 None:intergenic 35.0%
!! TTCATAGACTTGTTGCAAGT+TGG + chr8_1:3258248-3258267 Msa1173060:five_prime_UTR 35.0%
! CTAGTTTAGCCTTCTAGTAT+TGG + chr8_1:3258487-3258506 Msa1173060:intron 35.0%
CTAACAGATCCAATACTAGA+AGG - chr8_1:3258499-3258518 None:intergenic 35.0%
! TTTCATCATTTGGCTTATGG+AGG + chr8_1:3258746-3258765 Msa1173060:CDS 35.0%
! TACCAGTTCTTTTGAACAGT+TGG - chr8_1:3258892-3258911 None:intergenic 35.0%
! AGCCTTGAAAAAAGCTTTGA+AGG + chr8_1:3258911-3258930 Msa1173060:CDS 35.0%
! AAGTATGTTTGGTGGAACAA+GGG + chr8_1:3259194-3259213 Msa1173060:CDS 35.0%
ACACTAATTACCTTCTCTGT+TGG - chr8_1:3259252-3259271 None:intergenic 35.0%
!! CGGCTAGTTTAACAATATCA+CGG + chr8_1:3259287-3259306 Msa1173060:intron 35.0%
!!! AGCCTTTTCTAAAATCATGG+TGG + chr8_1:3259343-3259362 Msa1173060:intron 35.0%
!! GTCCACCATGATTTTAGAAA+AGG - chr8_1:3259348-3259367 None:intergenic 35.0%
! TTTTCCCAGCATTTTTCTCA+TGG - chr8_1:3259713-3259732 None:intergenic 35.0%
!! CATGTTTTTGCTTGCCATTA+TGG + chr8_1:3259805-3259824 Msa1173060:three_prime_UTR 35.0%
AAGCGTATTAATAGAGTTCG+TGG + chr8_1:3260006-3260025 Msa1173060:three_prime_UTR 35.0%
AACATAGCAACTTGTAGTTG+TGG + chr8_1:3260069-3260088 Msa1173060:three_prime_UTR 35.0%
ACATAGCAACTTGTAGTTGT+GGG + chr8_1:3260070-3260089 Msa1173060:three_prime_UTR 35.0%
! TTTTCAATTTCTGATGGCAC+TGG - chr8_1:3260159-3260178 None:intergenic 35.0%
! GTTGGTGAGGTAAGAAGATA+AGG + chr8_1:3258266-3258285 Msa1173060:five_prime_UTR 40.0%
GGAAAAAGAATGGCATTTGC+AGG + chr8_1:3258287-3258306 Msa1173060:exon 40.0%
! AGGAACAACTCAGAAGTGTA+TGG + chr8_1:3258307-3258326 Msa1173060:CDS 40.0%
! GACATCTGAAACAAGCTTTG+TGG - chr8_1:3258388-3258407 None:intergenic 40.0%
GACCAACTGTTCAAAAGAAC+TGG + chr8_1:3258887-3258906 Msa1173060:CDS 40.0%
GCTAAAGCCTCAAGTATGTT+TGG + chr8_1:3259183-3259202 Msa1173060:CDS 40.0%
AAAGCCTCAAGTATGTTTGG+TGG + chr8_1:3259186-3259205 Msa1173060:CDS 40.0%
TGTTCCACCAAACATACTTG+AGG - chr8_1:3259193-3259212 None:intergenic 40.0%
! CAAGTATGTTTGGTGGAACA+AGG + chr8_1:3259193-3259212 Msa1173060:CDS 40.0%
AACAGTGTATCCAACAGAGA+AGG + chr8_1:3259239-3259258 Msa1173060:CDS 40.0%
GTTCGGATTTGTGATGAGTT+TGG - chr8_1:3259382-3259401 None:intergenic 40.0%
TGCAACTTTCGCCAAAATCA+CGG - chr8_1:3259454-3259473 None:intergenic 40.0%
! AGTTGTTTCAAATGCTGCCA+TGG + chr8_1:3259573-3259592 Msa1173060:CDS 40.0%
! TCATGGGCTATGTAATTGGA+AGG - chr8_1:3259615-3259634 None:intergenic 40.0%
TTCCAATTACATAGCCCATG+AGG + chr8_1:3259614-3259633 Msa1173060:CDS 40.0%
TCCAATTACATAGCCCATGA+GGG + chr8_1:3259615-3259634 Msa1173060:CDS 40.0%
GCTTGATCAGTTGAATATGG+TGG - chr8_1:3259659-3259678 None:intergenic 40.0%
GTGACCATGAGAAAAATGCT+GGG + chr8_1:3259706-3259725 Msa1173060:CDS 40.0%
ACGGAAACCAAATGTAACTC+CGG + chr8_1:3259924-3259943 Msa1173060:three_prime_UTR 40.0%
AACCAAATGTAACTCCGGTA+TGG + chr8_1:3259929-3259948 Msa1173060:three_prime_UTR 40.0%
!!! TACATGGTTTTATTGGCGTC+TGG + chr8_1:3259959-3259978 Msa1173060:three_prime_UTR 40.0%
!! GGCACTGGTAAATGTAAGAA+AGG - chr8_1:3260144-3260163 None:intergenic 40.0%
! GTTGGTGAGGTAAGAAGATA+AGG + chr8_1:3258266-3258285 Msa1173060:five_prime_UTR 40.0%
GGAAAAAGAATGGCATTTGC+AGG + chr8_1:3258287-3258306 Msa1173060:exon 40.0%
! AGGAACAACTCAGAAGTGTA+TGG + chr8_1:3258307-3258326 Msa1173060:CDS 40.0%
! GACATCTGAAACAAGCTTTG+TGG - chr8_1:3258388-3258407 None:intergenic 40.0%
GACCAACTGTTCAAAAGAAC+TGG + chr8_1:3258887-3258906 Msa1173060:CDS 40.0%
GCTAAAGCCTCAAGTATGTT+TGG + chr8_1:3259183-3259202 Msa1173060:CDS 40.0%
AAAGCCTCAAGTATGTTTGG+TGG + chr8_1:3259186-3259205 Msa1173060:CDS 40.0%
TGTTCCACCAAACATACTTG+AGG - chr8_1:3259193-3259212 None:intergenic 40.0%
! CAAGTATGTTTGGTGGAACA+AGG + chr8_1:3259193-3259212 Msa1173060:CDS 40.0%
AACAGTGTATCCAACAGAGA+AGG + chr8_1:3259239-3259258 Msa1173060:CDS 40.0%
GTTCGGATTTGTGATGAGTT+TGG - chr8_1:3259382-3259401 None:intergenic 40.0%
TGCAACTTTCGCCAAAATCA+CGG - chr8_1:3259454-3259473 None:intergenic 40.0%
! AGTTGTTTCAAATGCTGCCA+TGG + chr8_1:3259573-3259592 Msa1173060:CDS 40.0%
! TCATGGGCTATGTAATTGGA+AGG - chr8_1:3259615-3259634 None:intergenic 40.0%
TTCCAATTACATAGCCCATG+AGG + chr8_1:3259614-3259633 Msa1173060:CDS 40.0%
TCCAATTACATAGCCCATGA+GGG + chr8_1:3259615-3259634 Msa1173060:CDS 40.0%
GCTTGATCAGTTGAATATGG+TGG - chr8_1:3259659-3259678 None:intergenic 40.0%
GTGACCATGAGAAAAATGCT+GGG + chr8_1:3259706-3259725 Msa1173060:CDS 40.0%
ACGGAAACCAAATGTAACTC+CGG + chr8_1:3259924-3259943 Msa1173060:three_prime_UTR 40.0%
AACCAAATGTAACTCCGGTA+TGG + chr8_1:3259929-3259948 Msa1173060:three_prime_UTR 40.0%
!!! TACATGGTTTTATTGGCGTC+TGG + chr8_1:3259959-3259978 Msa1173060:three_prime_UTR 40.0%
!! GGCACTGGTAAATGTAAGAA+AGG - chr8_1:3260144-3260163 None:intergenic 40.0%
!! AGACTTGTTGCAAGTTGGTG+AGG + chr8_1:3258253-3258272 Msa1173060:five_prime_UTR 45.0%
TTCAGATGTCACCACTGCAA+AGG + chr8_1:3258398-3258417 Msa1173060:CDS 45.0%
GAGAGAGACATAGACCTTGA+GGG - chr8_1:3258426-3258445 None:intergenic 45.0%
TGAGAGAGACATAGACCTTG+AGG - chr8_1:3258427-3258446 None:intergenic 45.0%
TTGAACAGTTGGTCGAAGTG+TGG - chr8_1:3258881-3258900 None:intergenic 45.0%
GGAACAAGGGACAAATGTTC+TGG + chr8_1:3259207-3259226 Msa1173060:CDS 45.0%
GATGAGTTTGGCAGAATCTG+TGG - chr8_1:3259370-3259389 None:intergenic 45.0%
! TCATTGTGCGACCGTGATTT+TGG + chr8_1:3259440-3259459 Msa1173060:intron 45.0%
TGTTTCAAATGCTGCCATGG+AGG + chr8_1:3259576-3259595 Msa1173060:CDS 45.0%
GTTTCAAATGCTGCCATGGA+GGG + chr8_1:3259577-3259596 Msa1173060:CDS 45.0%
GACTAATAGTACACCCTCCA+TGG - chr8_1:3259593-3259612 None:intergenic 45.0%
! TCCCTCATGGGCTATGTAAT+TGG - chr8_1:3259619-3259638 None:intergenic 45.0%
! GCAGTAGAGTTTTCCCTCAT+GGG - chr8_1:3259631-3259650 None:intergenic 45.0%
! TGCAGTAGAGTTTTCCCTCA+TGG - chr8_1:3259632-3259651 None:intergenic 45.0%
GGAAATTTGAGCCAGCTTGA+AGG + chr8_1:3259684-3259703 Msa1173060:CDS 45.0%
GGTGACCATGAGAAAAATGC+TGG + chr8_1:3259705-3259724 Msa1173060:CDS 45.0%
CTCCATACCGGAGTTACATT+TGG - chr8_1:3259934-3259953 None:intergenic 45.0%
CCATGTAACGAACTCCATAC+CGG - chr8_1:3259946-3259965 None:intergenic 45.0%
! CTGGACTCTGATGCATCTTT+TGG + chr8_1:3259978-3259997 Msa1173060:three_prime_UTR 45.0%
ACTTGTAGTTGTGGGTGAAC+AGG + chr8_1:3260078-3260097 Msa1173060:three_prime_UTR 45.0%
!! AGACTTGTTGCAAGTTGGTG+AGG + chr8_1:3258253-3258272 Msa1173060:five_prime_UTR 45.0%
TTCAGATGTCACCACTGCAA+AGG + chr8_1:3258398-3258417 Msa1173060:CDS 45.0%
GAGAGAGACATAGACCTTGA+GGG - chr8_1:3258426-3258445 None:intergenic 45.0%
TGAGAGAGACATAGACCTTG+AGG - chr8_1:3258427-3258446 None:intergenic 45.0%
TTGAACAGTTGGTCGAAGTG+TGG - chr8_1:3258881-3258900 None:intergenic 45.0%
GGAACAAGGGACAAATGTTC+TGG + chr8_1:3259207-3259226 Msa1173060:CDS 45.0%
GATGAGTTTGGCAGAATCTG+TGG - chr8_1:3259370-3259389 None:intergenic 45.0%
! TCATTGTGCGACCGTGATTT+TGG + chr8_1:3259440-3259459 Msa1173060:intron 45.0%
TGTTTCAAATGCTGCCATGG+AGG + chr8_1:3259576-3259595 Msa1173060:CDS 45.0%
GTTTCAAATGCTGCCATGGA+GGG + chr8_1:3259577-3259596 Msa1173060:CDS 45.0%
GACTAATAGTACACCCTCCA+TGG - chr8_1:3259593-3259612 None:intergenic 45.0%
! TCCCTCATGGGCTATGTAAT+TGG - chr8_1:3259619-3259638 None:intergenic 45.0%
! GCAGTAGAGTTTTCCCTCAT+GGG - chr8_1:3259631-3259650 None:intergenic 45.0%
! TGCAGTAGAGTTTTCCCTCA+TGG - chr8_1:3259632-3259651 None:intergenic 45.0%
GGAAATTTGAGCCAGCTTGA+AGG + chr8_1:3259684-3259703 Msa1173060:CDS 45.0%
GGTGACCATGAGAAAAATGC+TGG + chr8_1:3259705-3259724 Msa1173060:CDS 45.0%
CTCCATACCGGAGTTACATT+TGG - chr8_1:3259934-3259953 None:intergenic 45.0%
CCATGTAACGAACTCCATAC+CGG - chr8_1:3259946-3259965 None:intergenic 45.0%
! CTGGACTCTGATGCATCTTT+TGG + chr8_1:3259978-3259997 Msa1173060:three_prime_UTR 45.0%
ACTTGTAGTTGTGGGTGAAC+AGG + chr8_1:3260078-3260097 Msa1173060:three_prime_UTR 45.0%
TCTCATGGTCACCTTCAAGC+TGG - chr8_1:3259698-3259717 None:intergenic 50.0%
CCGGTATGGAGTTCGTTACA+TGG + chr8_1:3259943-3259962 Msa1173060:three_prime_UTR 50.0%
TCTCATGGTCACCTTCAAGC+TGG - chr8_1:3259698-3259717 None:intergenic 50.0%
CCGGTATGGAGTTCGTTACA+TGG + chr8_1:3259943-3259962 Msa1173060:three_prime_UTR 50.0%
CCTTGAGGGTTCCTTTGCAG+TGG - chr8_1:3258412-3258431 None:intergenic 55.0%
CCACTGCAAAGGAACCCTCA+AGG + chr8_1:3258409-3258428 Msa1173060:CDS 55.0%
! CTTGAGGCTTTAGCTGCTGC+AGG - chr8_1:3259177-3259196 None:intergenic 55.0%
CCTTGAGGGTTCCTTTGCAG+TGG - chr8_1:3258412-3258431 None:intergenic 55.0%
CCACTGCAAAGGAACCCTCA+AGG + chr8_1:3258409-3258428 Msa1173060:CDS 55.0%
! CTTGAGGCTTTAGCTGCTGC+AGG - chr8_1:3259177-3259196 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 3258086 3260187 3258086 ID=Msa1173060;Name=Msa1173060
chr8_1 mRNA 3258086 3260187 3258086 ID=Msa1173060-mRNA-1;Parent=Msa1173060;Name=Msa1173060-mRNA-1;_AED=0.15;_eAED=0.15;_QI=210|0.6|0.66|1|1|1|6|423|207
chr8_1 exon 3258086 3258430 3258086 ID=Msa1173060-mRNA-1:exon:1162;Parent=Msa1173060-mRNA-1
chr8_1 exon 3258691 3258767 3258691 ID=Msa1173060-mRNA-1:exon:1163;Parent=Msa1173060-mRNA-1
chr8_1 exon 3258877 3258932 3258877 ID=Msa1173060-mRNA-1:exon:1164;Parent=Msa1173060-mRNA-1
chr8_1 exon 3259029 3259072 3259029 ID=Msa1173060-mRNA-1:exon:1165;Parent=Msa1173060-mRNA-1
chr8_1 exon 3259171 3259260 3259171 ID=Msa1173060-mRNA-1:exon:1166;Parent=Msa1173060-mRNA-1
chr8_1 exon 3259543 3260187 3259543 ID=Msa1173060-mRNA-1:exon:1167;Parent=Msa1173060-mRNA-1
chr8_1 five_prime_UTR 3258086 3258295 3258086 ID=Msa1173060-mRNA-1:five_prime_utr;Parent=Msa1173060-mRNA-1
chr8_1 CDS 3258296 3258430 3258296 ID=Msa1173060-mRNA-1:cds;Parent=Msa1173060-mRNA-1
chr8_1 CDS 3258691 3258767 3258691 ID=Msa1173060-mRNA-1:cds;Parent=Msa1173060-mRNA-1
chr8_1 CDS 3258877 3258932 3258877 ID=Msa1173060-mRNA-1:cds;Parent=Msa1173060-mRNA-1
chr8_1 CDS 3259029 3259072 3259029 ID=Msa1173060-mRNA-1:cds;Parent=Msa1173060-mRNA-1
chr8_1 CDS 3259171 3259260 3259171 ID=Msa1173060-mRNA-1:cds;Parent=Msa1173060-mRNA-1
chr8_1 CDS 3259543 3259764 3259543 ID=Msa1173060-mRNA-1:cds;Parent=Msa1173060-mRNA-1
chr8_1 three_prime_UTR 3259765 3260187 3259765 ID=Msa1173060-mRNA-1:three_prime_utr;Parent=Msa1173060-mRNA-1
Gene Sequence

>Msa1173060

ATGGCATTTGCAGGAACAACTCAGAAGTGTATGGCTTGTAGCAAAACAGTTTATCTTGTTGATAAGTTAACTGCTGATAATAGAATTTTCCACAAAGCTTGTTTCAGATGTCACCACTGCAAAGGAACCCTCAAGTTGTTCATAAGCATTTTCATCTCAGACCACCGTAATTTTCATGTTAATGAACTTCTTTCATCATTTGGCTTATGGAGACCACACTTCGACCAACTGTTCAAAAGAACTGGTAGCCTTGAAAAAAGCTTTGAAGGGACACCAAAAATTGTCAAACCAGAAAGAAATATAGATAATGAGAAACCTGCAGCAGCTAAAGCCTCAAGTATGTTTGGTGGAACAAGGGACAAATGTTCTGGTTGTCAGAAAACAGTGTATCCAACAGAGAAGGTTACAGTGAATGGAACTCCTTACCATAAGAGTTGTTTCAAATGCTGCCATGGAGGGTGTACTATTAGTCCTTCCAATTACATAGCCCATGAGGGAAAACTCTACTGCAAACACCACCATATTCAACTGATCAAGCAAAAGGGAAATTTGAGCCAGCTTGAAGGTGACCATGAGAAAAATGCTGGGAAAATCAATGGCGAAGAAGTTGCAGCTGAAACTTGA
Protein sequence

>Msa1173060

MAFAGTTQKCMACSKTVYLVDKLTADNRIFHKACFRCHHCKGTLKLFISIFISDHRNFHVNELLSSFGLWRPHFDQLFKRTGSLEKSFEGTPKIVKPERNIDNEKPAAAKASSMFGGTRDKCSGCQKTVYPTEKVTVNGTPYHKSCFKCCHGGCTISPSNYIAHEGKLYCKHHHIQLIKQKGNLSQLEGDHEKNAGKINGEEVAAET*