AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1174410


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1174410 MtrunA17_Chr8g0344021 89.623 212 22 0 1 212 1 212 6.87e-136 379
Msa1174410 MtrunA17_Chr8g0344051 68.075 213 66 2 1 212 1 212 6.69e-101 291
Msa1174410 MtrunA17_Chr4g0003361 64.455 211 68 5 3 212 1 205 1.48e-84 249
Msa1174410 MtrunA17_Chr2g0286171 60.185 216 78 4 1 212 1 212 3.66e-81 241
Msa1174410 MtrunA17_Chr2g0301611 56.604 212 89 3 1 212 1 209 4.27e-80 238
Msa1174410 MtrunA17_Chr8g0344031 60.094 213 82 3 1 212 1 211 6.62e-80 238
Msa1174410 MtrunA17_Chr3g0115141 58.411 214 83 3 1 212 1 210 2.02e-79 236
Msa1174410 MtrunA17_Chr3g0084531 56.132 212 90 3 1 212 1 209 6.35e-79 235
Msa1174410 MtrunA17_Chr8g0343934 54.717 212 93 3 1 212 1 209 2.96e-78 233
Msa1174410 MtrunA17_Chr8g0344041 55.660 212 91 2 1 212 5 213 1.90e-77 231
Msa1174410 MtrunA17_Chr2g0297231 55.981 209 89 3 1 209 1 206 4.17e-77 230
Msa1174410 MtrunA17_Chr2g0297241 55.450 211 91 3 1 211 1 208 3.75e-76 228
Msa1174410 MtrunA17_Chr3g0084521 56.132 212 89 4 1 212 1 208 4.27e-76 228
Msa1174410 MtrunA17_Chr3g0084511 55.660 212 90 4 1 212 1 208 5.62e-76 227
Msa1174410 MtrunA17_Chr2g0313961 54.502 211 93 3 1 211 1 208 9.82e-75 224
Msa1174410 MtrunA17_Chr8g0343954 55.189 212 92 3 1 212 1 209 6.72e-73 219
Msa1174410 MtrunA17_Chr2g0302171 53.302 212 96 3 1 212 1 209 2.31e-72 218
Msa1174410 MtrunA17_Chr2g0302541 54.245 212 94 3 1 212 1 209 5.18e-72 217
Msa1174410 MtrunA17_Chr7g0217811 54.245 212 94 3 1 212 1 209 1.43e-71 216
Msa1174410 MtrunA17_Chr5g0411961 52.358 212 98 3 1 212 1 209 2.16e-71 216
Msa1174410 MtrunA17_Chr4g0012651 53.774 212 95 3 1 212 1 209 1.82e-70 213
Msa1174410 MtrunA17_Chr8g0343961 50.943 212 101 3 1 212 1 209 9.97e-69 209
Msa1174410 MtrunA17_Chr2g0300561 47.887 213 107 3 1 212 1 210 2.39e-64 198
Msa1174410 MtrunA17_Chr8g0343921 49.758 207 100 3 7 212 4 207 9.69e-64 196
Msa1174410 MtrunA17_Chr2g0300581 45.070 213 113 3 1 212 1 210 5.77e-63 194
Msa1174410 MtrunA17_Chr2g0300591 44.131 213 115 3 1 212 1 210 4.24e-62 192
Msa1174410 MtrunA17_Chr8g0343911 47.867 211 106 4 3 212 1 208 6.94e-62 191
Msa1174410 MtrunA17_Chr3g0084551 45.366 205 103 4 1 205 1 196 3.93e-52 167
Msa1174410 MtrunA17_Chr6g0449751 41.284 218 107 6 6 208 4 215 3.27e-41 139
Msa1174410 MtrunA17_Chr6g0449731 41.014 217 107 6 7 208 5 215 3.19e-40 137
Msa1174410 MtrunA17_Chr6g0449761 40.741 216 107 6 8 208 6 215 1.22e-39 136
Msa1174410 MtrunA17_Chr6g0449771 40.553 217 108 6 7 208 5 215 1.85e-39 135
Msa1174410 MtrunA17_Chr2g0292831 41.885 191 101 3 16 203 19 202 3.54e-37 129
Msa1174410 MtrunA17_Chr1g0189841 38.739 222 117 6 1 208 1 217 3.61e-37 129
Msa1174410 MtrunA17_Chr1g0193421 40.722 194 111 3 15 205 20 212 5.24e-37 128
Msa1174410 MtrunA17_Chr2g0292901 41.579 190 102 3 24 208 28 213 1.68e-36 127
Msa1174410 MtrunA17_Chr1g0156131 41.237 194 101 3 13 203 16 199 3.80e-36 126
Msa1174410 MtrunA17_Chr6g0449791 44.318 176 86 4 8 173 6 179 1.51e-35 124
Msa1174410 MtrunA17_Chr7g0271211 44.706 170 92 2 1 169 2 170 3.61e-35 124
Msa1174410 MtrunA17_Chr4g0007041 39.352 216 108 6 1 203 1 206 7.90e-35 123
Msa1174410 MtrunA17_Chr1g0178711 34.653 202 124 3 11 205 18 218 1.28e-34 122
Msa1174410 MtrunA17_Chr1g0193671 38.835 206 118 5 6 205 8 211 2.78e-34 121
Msa1174410 MtrunA17_Chr4g0007051 39.352 216 108 6 1 203 1 206 2.80e-34 121
Msa1174410 MtrunA17_Chr2g0285871 39.815 216 107 6 1 203 1 206 2.83e-34 121
Msa1174410 MtrunA17_Chr4g0007031 39.352 216 108 6 1 203 1 206 3.15e-34 121
Msa1174410 MtrunA17_Chr5g0419971 39.352 216 108 6 1 203 1 206 3.15e-34 121
Msa1174410 MtrunA17_Chr5g0419981 39.352 216 108 6 1 203 1 206 3.15e-34 121
Msa1174410 MtrunA17_Chr5g0414971 35.897 195 112 5 24 210 32 221 1.52e-33 119
Msa1174410 MtrunA17_Chr2g0292861 42.424 165 88 3 43 203 7 168 5.65e-31 112
Msa1174410 MtrunA17_Chr1g0210761 33.175 211 130 5 4 211 8 210 9.70e-31 112
Msa1174410 MtrunA17_Chr6g0449781 37.089 213 122 5 6 208 4 214 1.62e-29 109
Msa1174410 MtrunA17_Chr2g0292841 42.424 165 88 3 43 203 7 168 1.95e-29 107
Msa1174410 MtrunA17_Chr8g0335851 44.030 134 73 2 39 171 47 179 1.02e-28 107
Msa1174410 MtrunA17_Chr2g0292911 47.863 117 58 1 43 156 7 123 2.08e-28 104
Msa1174410 MtrunA17_Chr3g0084501 49.074 108 52 3 1 108 1 105 4.69e-26 97.1
Msa1174410 MtrunA17_Chr3g0130241 38.235 136 82 2 39 173 110 244 8.37e-25 98.6
Msa1174410 MtrunA17_Chr2g0321581 38.931 131 78 2 80 208 74 204 2.53e-21 87.4
Msa1174410 MtrunA17_Chr2g0321591 38.931 131 78 2 80 208 74 204 3.74e-21 87.0
Msa1174410 MtrunA17_Chr4g0026191 38.060 134 81 2 77 208 72 205 4.72e-20 84.3
Msa1174410 MtrunA17_Chr4g0026201 38.060 134 81 2 77 208 72 205 5.64e-20 84.0
Msa1174410 MtrunA17_Chr2g0321601 37.594 133 79 4 79 208 72 203 6.34e-20 84.0
Msa1174410 MtrunA17_Chr2g0327651 37.755 98 60 1 50 146 10 107 4.52e-16 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1174410 AT5G20630.1 46.698 212 110 3 1 211 1 210 1.34e-59 186
Msa1174410 AT1G72610.1 47.368 190 98 2 24 212 20 208 2.53e-57 180
Msa1174410 AT5G38960.1 43.564 202 102 4 20 209 22 223 6.55e-44 147
Msa1174410 AT5G38940.1 46.154 182 91 3 24 201 31 209 2.34e-42 142
Msa1174410 AT5G38940.2 46.154 182 91 3 24 201 55 233 5.39e-42 142
Msa1174410 AT3G04200.1 42.188 192 105 2 24 209 32 223 6.26e-42 142
Msa1174410 AT5G39160.3 38.636 220 120 5 1 209 1 216 3.56e-41 139
Msa1174410 AT5G39190.1 38.636 220 124 4 1 209 1 220 3.69e-41 139
Msa1174410 AT5G39160.1 38.636 220 124 4 1 209 1 220 4.11e-41 139
Msa1174410 AT3G05950.1 42.632 190 103 2 25 208 32 221 2.53e-40 137
Msa1174410 AT5G39130.1 38.182 220 125 4 1 209 1 220 5.23e-40 136
Msa1174410 AT5G39110.1 41.885 191 104 3 25 208 30 220 5.46e-40 136
Msa1174410 AT5G38930.1 46.341 164 84 2 25 184 32 195 1.37e-39 135
Msa1174410 AT4G14630.1 41.837 196 97 4 25 208 32 222 2.44e-39 135
Msa1174410 AT5G39120.1 39.450 218 111 6 6 208 8 219 5.11e-39 134
Msa1174410 AT5G39180.1 38.182 220 119 4 1 208 5 219 3.26e-38 132
Msa1174410 AT5G26700.1 40.196 204 115 4 3 205 9 206 3.79e-38 131
Msa1174410 AT5G39150.1 38.182 220 119 4 1 208 5 219 9.88e-38 130
Msa1174410 AT5G38910.1 45.930 172 75 6 24 184 29 193 4.62e-37 129
Msa1174410 AT3G04150.1 35.023 217 133 3 2 212 11 225 6.25e-37 129
Msa1174410 AT1G02335.1 36.842 209 125 4 4 206 6 213 2.31e-36 127
Msa1174410 AT1G09560.1 44.571 175 92 3 16 186 18 191 4.60e-36 126
Msa1174410 AT3G04150.2 37.500 176 104 2 43 212 63 238 2.00e-35 125
Msa1174410 AT3G04190.1 38.220 191 112 2 24 208 31 221 2.86e-35 124
Msa1174410 AT3G05930.1 43.386 189 102 4 24 208 28 215 3.04e-35 124
Msa1174410 AT1G18980.1 37.379 206 123 4 6 208 16 218 4.96e-35 124
Msa1174410 AT5G39190.2 37.931 174 102 2 42 209 25 198 5.93e-34 120
Msa1174410 AT5G39160.2 37.931 174 102 2 42 209 25 198 6.68e-34 120
Msa1174410 AT1G18970.1 37.264 212 124 5 3 208 10 218 1.09e-33 120
Msa1174410 AT5G61750.1 37.430 179 102 3 1 173 1 175 2.14e-33 119
Msa1174410 AT3G62020.1 36.842 209 122 5 9 208 8 215 3.36e-33 119
Msa1174410 AT3G04180.1 36.842 190 114 2 25 208 32 221 8.12e-32 115
Msa1174410 AT3G04170.1 37.895 190 109 4 25 208 32 218 3.84e-31 114
Msa1174410 AT3G62020.2 37.931 174 104 3 39 208 13 186 5.90e-31 112
Msa1174410 AT1G10460.1 34.536 194 107 6 25 203 25 213 4.99e-28 105
Msa1174410 AT3G10080.1 33.854 192 117 4 24 208 31 219 1.26e-26 102
Msa1174410 AT3G10080.2 33.854 192 117 4 24 208 102 290 5.13e-26 102
Msa1174410 AT1G74820.1 39.024 164 86 5 4 156 11 171 1.18e-25 99.8
Msa1174410 AT5G38910.2 36.905 168 57 6 25 184 30 156 1.16e-20 85.5

Find 34 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAACTAGTGCTTTGTTATT+TGG 0.151155 8_1:-5507183 Msa1174410:CDS
TGAAAATGCAATAGTCCTTT+TGG 0.222682 8_1:+5507273 None:intergenic
AGAATAGATATTGGTATAAA+TGG 0.231195 8_1:-5507429 Msa1174410:CDS
GAGGTTAATCCAACTTTAAA+AGG 0.242984 8_1:+5507504 None:intergenic
CTCTTAATCCCGGTGATGTT+TGG 0.254070 8_1:-5507302 Msa1174410:CDS
TATCACACCTACAAAACTTT+TGG 0.293680 8_1:-5507331 Msa1174410:CDS
TTCTTGATGCTGCACAAATT+AGG 0.325122 8_1:-5507122 Msa1174410:CDS
TCTTAATCCCGGTGATGTTT+GGG 0.351443 8_1:-5507301 Msa1174410:CDS
CTTTCATCAGACACTCCTTC+AGG 0.357904 8_1:-5507609 Msa1174410:CDS
CATTGGAGGTAAATGAGATT+AGG 0.367587 8_1:+5507670 None:intergenic
TCTGCAGCAAGAATAGATAT+TGG 0.394290 8_1:-5507438 Msa1174410:CDS
AGGAAGCTTAAGGATGTGTT+TGG 0.401730 8_1:-5507102 Msa1174410:CDS
ATTGGAGGTAAATGAGATTA+GGG 0.404625 8_1:+5507671 None:intergenic
ATTAGTAATTCAGTTGCATC+AGG 0.442131 8_1:+5507384 None:intergenic
TACTCTAGCTTAGCAGCTGC+TGG 0.445392 8_1:-5507540 Msa1174410:CDS
GAATTACTAATCGTTGTTCA+AGG 0.451387 8_1:-5507372 Msa1174410:CDS
AAGGATGTGTTTGGTGGCAG+TGG 0.462003 8_1:-5507093 Msa1174410:CDS
TTTCAAGTTAACTCTGGTGC+TGG 0.464652 8_1:-5507255 Msa1174410:CDS
TGCACAAATTAGGAAGCTTA+AGG 0.487134 8_1:-5507112 Msa1174410:CDS
GCACTAGTTACATGTATGCT+AGG 0.496669 8_1:+5507195 None:intergenic
GTTCAAGGTCAATTTACTGT+TGG 0.503571 8_1:-5507357 Msa1174410:CDS
AGTAATTTACCAGGTCTCAA+TGG 0.507923 8_1:-5507471 Msa1174410:CDS
AGGTTAATCCAACTTTAAAA+GGG 0.508517 8_1:+5507505 None:intergenic
ATCATCAGAGTCAATAGCAT+TGG 0.511591 8_1:+5507653 None:intergenic
ATCAGAGTCAATAGCATTGG+AGG 0.512491 8_1:+5507656 None:intergenic
AAGCTTAAGGATGTGTTTGG+TGG 0.535264 8_1:-5507099 Msa1174410:CDS
TGTGTTTGGTGGCAGTGGCT+AGG 0.536076 8_1:-5507088 None:intergenic
CACTAGTTACATGTATGCTA+GGG 0.551616 8_1:+5507196 None:intergenic
TTGGTGAAAACTCTTAATCC+CGG 0.558325 8_1:-5507312 Msa1174410:CDS
GATTTGCATGGATAGCCTGA+AGG 0.619224 8_1:+5507594 None:intergenic
GTAAATGTCAGTAATTTACC+AGG 0.629129 8_1:-5507480 Msa1174410:CDS
GCATCAGGATGAGTGTGCAT+TGG 0.654477 8_1:+5507399 None:intergenic
AAACTAAGTCCATTGAGACC+TGG 0.656424 8_1:+5507462 None:intergenic
TAAATTACTGACATTTACCG+AGG 0.660517 8_1:+5507485 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATAGATATTGGTATAAA+TGG - chr8_1:5507365-5507384 Msa1174410:CDS 20.0%
!!! ACAAATTTTTCTGATTTGCA+TGG + chr8_1:5507215-5507234 None:intergenic 25.0%
!! AGGTTAATCCAACTTTAAAA+GGG + chr8_1:5507292-5507311 None:intergenic 25.0%
!!! TTTTAAAGTTGGATTAACCT+CGG - chr8_1:5507292-5507311 Msa1174410:CDS 25.0%
ATTGGAGGTAAATGAGATTA+GGG + chr8_1:5507126-5507145 None:intergenic 30.0%
GAGGTTAATCCAACTTTAAA+AGG + chr8_1:5507293-5507312 None:intergenic 30.0%
TAAATTACTGACATTTACCG+AGG + chr8_1:5507312-5507331 None:intergenic 30.0%
GTAAATGTCAGTAATTTACC+AGG - chr8_1:5507314-5507333 Msa1174410:CDS 30.0%
ATTAGTAATTCAGTTGCATC+AGG + chr8_1:5507413-5507432 None:intergenic 30.0%
GAATTACTAATCGTTGTTCA+AGG - chr8_1:5507422-5507441 Msa1174410:CDS 30.0%
! TATCACACCTACAAAACTTT+TGG - chr8_1:5507463-5507482 Msa1174410:CDS 30.0%
!! GTTTTCACCAAAAGTTTTGT+AGG + chr8_1:5507473-5507492 None:intergenic 30.0%
! TGAAAATGCAATAGTCCTTT+TGG + chr8_1:5507524-5507543 None:intergenic 30.0%
!!! TTGCATTTTCAAGTTAACTC+TGG - chr8_1:5507533-5507552 Msa1174410:CDS 30.0%
!! GTAACTAGTGCTTTGTTATT+TGG - chr8_1:5507611-5507630 Msa1174410:CDS 30.0%
! AAGAGTAGTCTTTTGAATTG+TGG + chr8_1:5507654-5507673 None:intergenic 30.0%
CATTGGAGGTAAATGAGATT+AGG + chr8_1:5507127-5507146 None:intergenic 35.0%
ATCATCAGAGTCAATAGCAT+TGG + chr8_1:5507144-5507163 None:intergenic 35.0%
!!! ACAGCAAACCCTTTTAAAGT+TGG - chr8_1:5507281-5507300 Msa1174410:CDS 35.0%
AGTAATTTACCAGGTCTCAA+TGG - chr8_1:5507323-5507342 Msa1174410:CDS 35.0%
TCTGCAGCAAGAATAGATAT+TGG - chr8_1:5507356-5507375 Msa1174410:CDS 35.0%
GTTCAAGGTCAATTTACTGT+TGG - chr8_1:5507437-5507456 Msa1174410:CDS 35.0%
TTGGTGAAAACTCTTAATCC+CGG - chr8_1:5507482-5507501 Msa1174410:CDS 35.0%
!! GATGTTTGGGTTTTTCCAAA+AGG - chr8_1:5507506-5507525 Msa1174410:CDS 35.0%
CACTAGTTACATGTATGCTA+GGG + chr8_1:5507601-5507620 None:intergenic 35.0%
! GTCTTTTGAATTGTGGAAGT+TGG + chr8_1:5507647-5507666 None:intergenic 35.0%
TTCTTGATGCTGCACAAATT+AGG - chr8_1:5507672-5507691 Msa1174410:CDS 35.0%
TGCACAAATTAGGAAGCTTA+AGG - chr8_1:5507682-5507701 Msa1174410:CDS 35.0%
! ATCAGAGTCAATAGCATTGG+AGG + chr8_1:5507141-5507160 None:intergenic 40.0%
AAACTAAGTCCATTGAGACC+TGG + chr8_1:5507335-5507354 None:intergenic 40.0%
TCTTAATCCCGGTGATGTTT+GGG - chr8_1:5507493-5507512 Msa1174410:CDS 40.0%
TGGAAAAACCCAAACATCAC+CGG + chr8_1:5507504-5507523 None:intergenic 40.0%
!! TTTCAAGTTAACTCTGGTGC+TGG - chr8_1:5507539-5507558 Msa1174410:CDS 40.0%
GCACTAGTTACATGTATGCT+AGG + chr8_1:5507602-5507621 None:intergenic 40.0%
AGGAAGCTTAAGGATGTGTT+TGG - chr8_1:5507692-5507711 Msa1174410:CDS 40.0%
AAGCTTAAGGATGTGTTTGG+TGG - chr8_1:5507695-5507714 Msa1174410:CDS 40.0%
CTTTCATCAGACACTCCTTC+AGG - chr8_1:5507185-5507204 Msa1174410:CDS 45.0%
GATTTGCATGGATAGCCTGA+AGG + chr8_1:5507203-5507222 None:intergenic 45.0%
CTCTTAATCCCGGTGATGTT+TGG - chr8_1:5507492-5507511 Msa1174410:CDS 45.0%
GGAAAAACCCAAACATCACC+GGG + chr8_1:5507503-5507522 None:intergenic 45.0%
! TACTCTAGCTTAGCAGCTGC+TGG - chr8_1:5507254-5507273 Msa1174410:CDS 50.0%
GCATCAGGATGAGTGTGCAT+TGG + chr8_1:5507398-5507417 None:intergenic 50.0%
! AAGGATGTGTTTGGTGGCAG+TGG - chr8_1:5507701-5507720 Msa1174410:CDS 50.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 5507089 5507727 5507089 ID=Msa1174410;Name=Msa1174410
chr8_1 mRNA 5507089 5507727 5507089 ID=Msa1174410-mRNA-1;Parent=Msa1174410;Name=Msa1174410-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|212
chr8_1 exon 5507089 5507727 5507089 ID=Msa1174410-mRNA-1:exon:1853;Parent=Msa1174410-mRNA-1
chr8_1 CDS 5507089 5507727 5507089 ID=Msa1174410-mRNA-1:cds;Parent=Msa1174410-mRNA-1
Gene Sequence

>Msa1174410

ATGAAGATAATTCAAATTATTCTTTTCTTTTTTGCCCTAATCTCATTTACCTCCAATGCTATTGACTCTGATGATTTCTGTGTAGCAAACTTATTTCTTTCATCAGACACTCCTTCAGGCTATCCATGCAAATCAGAAAAATTTGTAACAGCGAATGATTTCGCGTACTCTAGCTTAGCAGCTGCTGGAAGCACAGCAAACCCTTTTAAAGTTGGATTAACCTCGGTAAATGTCAGTAATTTACCAGGTCTCAATGGACTTAGTTTATCTGCAGCAAGAATAGATATTGGTATAAATGGAAGTGTACCAATGCACACTCATCCTGATGCAACTGAATTACTAATCGTTGTTCAAGGTCAATTTACTGTTGGATTTATCACACCTACAAAACTTTTGGTGAAAACTCTTAATCCCGGTGATGTTTGGGTTTTTCCAAAAGGACTATTGCATTTTCAAGTTAACTCTGGTGCTGGAAATGCTATTGCTTATGCTGCTTTTAGTAGCTCAAACCCTAGCATACATGTAACTAGTGCTTTGTTATTTGGTAACAACTTACCAACTTCCACAATTCAAAAGACTACTCTTCTTGATGCTGCACAAATTAGGAAGCTTAAGGATGTGTTTGGTGGCAGTGGCTAG

Protein sequence

>Msa1174410

MKIIQIILFFFALISFTSNAIDSDDFCVANLFLSSDTPSGYPCKSEKFVTANDFAYSSLAAAGSTANPFKVGLTSVNVSNLPGLNGLSLSAARIDIGINGSVPMHTHPDATELLIVVQGQFTVGFITPTKLLVKTLNPGDVWVFPKGLLHFQVNSGAGNAIAYAAFSSSNPSIHVTSALLFGNNLPTSTIQKTTLLDAAQIRKLKDVFGGSG*