AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0314630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0314630 MtrunA17_Chr2g0284921 60.345 232 82 1 4 225 129 360 1.03e-95 283
Msa0314630 MtrunA17_Chr2g0284991 52.743 237 100 2 1 225 123 359 1.68e-80 245
Msa0314630 MtrunA17_Chr2g0284951 52.321 237 96 4 4 228 126 357 2.48e-78 239
Msa0314630 MtrunA17_Chr3g0085881 52.586 232 96 3 4 225 125 352 2.72e-78 239
Msa0314630 MtrunA17_Chr2g0285001 49.791 239 108 2 2 228 125 363 3.13e-77 237
Msa0314630 MtrunA17_Chr8g0380031 46.320 231 113 5 4 225 177 405 8.50e-69 216
Msa0314630 MtrunA17_Chr2g0284941 47.899 238 93 3 2 228 124 341 1.51e-68 214
Msa0314630 MtrunA17_Chr2g0284931 47.660 235 96 2 4 228 5 222 1.02e-67 207
Msa0314630 MtrunA17_Chr8g0380041 42.739 241 118 5 4 225 123 362 3.85e-61 195
Msa0314630 MtrunA17_Chr2g0285061 38.174 241 129 5 4 225 125 364 2.84e-52 172
Msa0314630 MtrunA17_Chr3g0086641 38.174 241 128 7 4 225 131 369 2.58e-51 170
Msa0314630 MtrunA17_Chr3g0077571 39.070 215 109 5 29 225 146 356 2.59e-47 159
Msa0314630 MtrunA17_Chr2g0285041 87.805 82 10 0 4 85 42 123 1.66e-44 146
Msa0314630 MtrunA17_Chr2g0285031 77.922 77 17 0 153 229 5 81 1.62e-38 128
Msa0314630 MtrunA17_Chr3g0131321 35.510 245 132 8 4 225 127 368 3.77e-38 136
Msa0314630 MtrunA17_Chr3g0077581 33.745 243 136 7 4 224 129 368 1.88e-37 134
Msa0314630 MtrunA17_Chr7g0275011 36.410 195 106 4 24 200 148 342 4.11e-35 128
Msa0314630 MtrunA17_Chr6g0455661 35.096 208 115 4 35 225 267 471 9.77e-34 126
Msa0314630 MtrunA17_Chr6g0459921 33.333 240 139 5 4 225 124 360 1.07e-33 124
Msa0314630 MtrunA17_Chr7g0275021 32.821 195 109 3 24 198 148 340 1.94e-33 123
Msa0314630 MtrunA17_Chr4g0008851 34.928 209 110 5 24 206 146 354 2.07e-33 123
Msa0314630 MtrunA17_Chr5g0434621 34.112 214 121 5 12 208 129 339 4.08e-33 122
Msa0314630 MtrunA17_Chr4g0017431 32.018 228 131 5 20 225 137 362 6.23e-33 122
Msa0314630 MtrunA17_Chr7g0274991 33.028 218 126 4 24 223 147 362 8.31e-33 122
Msa0314630 MtrunA17_Chr7g0215961 33.516 182 103 2 37 200 147 328 9.72e-32 119
Msa0314630 MtrunA17_Chr1g0189901 32.536 209 117 4 11 195 126 334 6.73e-31 117
Msa0314630 MtrunA17_Chr1g0189891 32.984 191 116 2 20 198 140 330 1.34e-30 115
Msa0314630 MtrunA17_Chr6g0457921 33.333 192 110 2 27 200 147 338 3.23e-30 115
Msa0314630 MtrunA17_Chr7g0275001 32.524 206 111 5 14 198 134 332 9.20e-30 113
Msa0314630 MtrunA17_Chr1g0182811 32.812 192 109 4 29 200 153 344 2.53e-29 112
Msa0314630 MtrunA17_Chr4g0060811 34.951 206 107 6 16 202 140 337 3.74e-29 112
Msa0314630 MtrunA17_Chr1g0189931 32.432 185 107 3 29 195 151 335 4.10e-29 112
Msa0314630 MtrunA17_Chr4g0068441 31.839 223 129 7 24 225 147 367 1.14e-27 108
Msa0314630 MtrunA17_Chr3g0092131 32.812 192 109 4 29 202 144 333 1.96e-27 107
Msa0314630 MtrunA17_Chr3g0085231 34.300 207 105 5 36 225 157 349 4.57e-24 98.2
Msa0314630 MtrunA17_Chr5g0438621 30.348 201 113 7 26 202 143 340 8.18e-24 97.4
Msa0314630 MtrunA17_Chr5g0396511 26.778 239 153 6 4 223 141 376 1.75e-22 94.4
Msa0314630 MtrunA17_Chr2g0320671 28.571 217 130 7 7 202 128 340 4.09e-22 92.8
Msa0314630 MtrunA17_Chr2g0294301 30.500 200 118 6 24 202 147 346 4.11e-22 93.2
Msa0314630 MtrunA17_Chr2g0294311 27.928 222 137 6 24 225 154 372 5.16e-22 92.8
Msa0314630 MtrunA17_Chr8g0374101 25.941 239 152 8 7 223 138 373 1.89e-20 88.6
Msa0314630 MtrunA17_Chr5g0438631 28.272 191 114 4 29 198 147 335 7.65e-20 86.7
Msa0314630 MtrunA17_Chr8g0370961 25.974 231 134 8 20 224 24 243 1.53e-19 84.0
Msa0314630 MtrunA17_Chr1g0156911 27.751 209 126 7 19 204 143 349 5.95e-19 84.3
Msa0314630 MtrunA17_Chr8g0370951 27.624 181 104 7 37 195 164 339 7.43e-19 84.0
Msa0314630 MtrunA17_Chr5g0444401 26.250 240 149 8 3 223 129 359 2.55e-18 82.4
Msa0314630 MtrunA17_Chr3g0105401 29.279 222 131 7 5 202 120 339 4.08e-18 81.6
Msa0314630 MtrunA17_Chr3g0142921 26.961 204 121 6 18 197 142 341 1.38e-17 80.5
Msa0314630 MtrunA17_Chr1g0156891 28.358 201 120 6 19 197 150 348 3.26e-17 79.3
Msa0314630 MtrunA17_Chr5g0405821 29.016 193 112 5 37 207 172 361 4.05e-17 79.0
Msa0314630 MtrunA17_Chr5g0440501 23.529 221 141 6 31 225 32 250 9.23e-17 76.6
Msa0314630 MtrunA17_Chr5g0438641 29.082 196 113 7 29 202 151 342 1.91e-16 77.0
Msa0314630 MtrunA17_Chr2g0330781 26.728 217 135 6 7 202 129 342 2.05e-16 77.0
Msa0314630 MtrunA17_Chr1g0156931 27.000 200 119 7 19 195 147 342 2.68e-16 76.6
Msa0314630 MtrunA17_Chr8g0371471 26.000 200 123 6 19 195 147 344 2.82e-16 76.6
Msa0314630 MtrunA17_Chr3g0099161 28.095 210 120 7 35 223 224 423 3.01e-16 77.0
Msa0314630 MtrunA17_Chr3g0120861 29.744 195 113 6 29 202 157 348 3.01e-16 76.6
Msa0314630 MtrunA17_Chr1g0156901 25.116 215 137 6 5 197 142 354 3.06e-16 76.6
Msa0314630 MtrunA17_Chr1g0156921 27.778 180 102 5 41 197 173 347 7.28e-16 75.5
Msa0314630 MtrunA17_Chr3g0142941 26.733 202 122 7 19 197 153 351 1.12e-15 75.1
Msa0314630 MtrunA17_Chr2g0284981 52.308 65 31 0 161 225 15 79 1.25e-15 70.1
Msa0314630 MtrunA17_Chr4g0027521 26.633 199 119 5 24 198 141 336 1.68e-15 74.3
Msa0314630 MtrunA17_Chr8g0344931 25.523 239 154 7 4 225 129 360 3.55e-15 73.6
Msa0314630 MtrunA17_Chr4g0027571 27.692 195 119 5 24 198 14 206 4.01e-15 72.0
Msa0314630 MtrunA17_Chr3g0099151 27.536 207 124 7 36 223 220 419 9.56e-15 72.4
Msa0314630 MtrunA17_Chr3g0099231 26.066 211 131 7 36 228 160 363 1.17e-14 72.0
Msa0314630 MtrunA17_Chr5g0405831 27.083 192 117 6 37 207 171 360 5.25e-14 70.1
Msa0314630 MtrunA17_Chr8g0371481 25.373 201 124 7 19 195 147 345 7.36e-14 69.7
Msa0314630 MtrunA17_Chr4g0046151 25.000 220 133 7 4 195 131 346 8.09e-14 69.7
Msa0314630 MtrunA17_Chr2g0283191 24.272 206 116 7 27 195 148 350 1.68e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0314630 AT1G29670.1 41.250 240 122 4 4 225 124 362 1.12e-59 192
Msa0314630 AT1G29670.2 41.250 240 122 4 4 225 192 430 7.55e-59 192
Msa0314630 AT1G29660.1 40.664 241 123 4 4 225 124 363 1.46e-57 186
Msa0314630 AT5G45670.1 38.333 240 128 5 4 224 122 360 1.09e-52 174
Msa0314630 AT4G18970.1 39.004 241 127 5 4 225 121 360 8.94e-52 171
Msa0314630 AT4G18970.2 39.004 241 127 5 4 225 121 360 4.96e-51 171
Msa0314630 AT1G33811.1 35.102 245 132 7 4 224 127 368 2.84e-46 157
Msa0314630 AT1G71691.1 33.333 231 132 5 12 224 54 280 4.29e-45 152
Msa0314630 AT1G71691.2 33.333 231 132 5 12 224 155 381 8.42e-44 151
Msa0314630 AT2G19050.1 37.438 203 109 3 37 225 150 348 5.58e-39 138
Msa0314630 AT4G30140.1 34.764 233 127 5 4 223 126 346 7.05e-38 135
Msa0314630 AT2G19060.1 36.493 211 111 4 4 198 123 326 2.61e-37 134
Msa0314630 AT5G08460.1 31.967 244 141 6 4 225 144 384 1.53e-36 132
Msa0314630 AT1G71250.1 32.780 241 138 7 3 223 134 370 3.43e-36 131
Msa0314630 AT2G19010.1 33.898 236 131 4 4 225 123 347 6.99e-36 130
Msa0314630 AT5G15720.3 34.413 247 133 6 3 225 91 332 1.15e-35 129
Msa0314630 AT2G19010.2 33.898 236 131 4 4 225 153 377 1.45e-35 129
Msa0314630 AT5G15720.1 34.413 247 133 6 3 225 122 363 2.93e-35 128
Msa0314630 AT5G33370.1 30.222 225 134 5 20 223 137 359 5.20e-32 120
Msa0314630 AT2G19050.2 45.161 124 68 0 37 160 150 273 7.18e-31 115
Msa0314630 AT3G04290.1 31.863 204 115 4 37 220 156 355 3.55e-30 115
Msa0314630 AT1G71691.3 34.266 143 92 2 12 154 155 295 6.82e-27 105
Msa0314630 AT5G37690.2 29.947 187 112 4 33 202 143 327 7.70e-27 105
Msa0314630 AT5G37690.1 29.947 187 112 4 33 202 151 335 8.64e-27 105
Msa0314630 AT1G74460.1 32.828 198 113 5 23 202 138 333 2.13e-26 105
Msa0314630 AT4G28780.1 31.694 183 105 5 37 200 158 339 8.59e-26 103
Msa0314630 AT5G18430.1 30.000 210 118 6 24 206 143 350 2.72e-25 102
Msa0314630 AT5G15720.2 38.125 160 90 3 3 156 122 278 1.67e-24 99.0
Msa0314630 AT3G50400.1 33.000 200 111 8 26 202 153 352 4.82e-23 96.3
Msa0314630 AT3G53100.1 30.500 200 115 6 24 202 143 339 4.72e-21 90.5
Msa0314630 AT2G04570.1 30.256 195 114 5 28 202 146 338 1.13e-20 89.4
Msa0314630 AT4G10950.2 25.229 218 142 6 4 202 166 381 4.97e-20 88.2
Msa0314630 AT4G10950.1 25.229 218 142 6 4 202 162 377 5.40e-20 87.8
Msa0314630 AT5G33370.2 32.374 139 91 2 20 155 137 275 9.27e-20 86.7
Msa0314630 AT2G23540.1 27.778 198 121 6 27 202 168 365 1.42e-19 86.7
Msa0314630 AT5G22810.1 29.224 219 129 7 7 202 132 347 2.75e-19 85.5
Msa0314630 AT5G03820.1 27.982 218 132 6 7 202 131 345 3.68e-19 85.1
Msa0314630 AT5G03820.2 27.982 218 132 6 7 202 159 373 5.06e-19 85.1
Msa0314630 AT2G42990.1 30.890 191 108 6 29 198 147 334 7.99e-19 84.3
Msa0314630 AT4G26790.1 29.902 204 117 7 29 209 148 348 1.78e-18 83.2
Msa0314630 AT4G26790.2 29.902 204 117 7 29 209 148 348 1.78e-18 83.2
Msa0314630 AT2G03980.2 28.283 198 110 4 36 210 171 359 4.23e-18 82.4
Msa0314630 AT2G03980.3 28.283 198 110 4 36 210 171 359 4.23e-18 82.4
Msa0314630 AT2G03980.1 28.283 198 110 4 36 210 171 359 4.23e-18 82.4
Msa0314630 AT2G03980.4 28.283 198 110 4 36 210 171 359 4.23e-18 82.4
Msa0314630 AT2G30310.1 26.168 214 125 8 19 202 141 351 8.61e-18 81.3
Msa0314630 AT1G58430.1 25.352 213 131 7 10 197 133 342 1.12e-17 80.9
Msa0314630 AT5G63170.1 30.168 179 90 6 37 195 156 319 1.78e-17 80.1
Msa0314630 AT2G30220.1 25.926 216 123 9 19 202 140 350 2.31e-17 80.1
Msa0314630 AT3G16370.1 27.363 201 121 6 24 202 144 341 4.40e-17 79.3
Msa0314630 AT1G20130.1 31.217 189 105 6 26 194 330 513 1.94e-16 78.2
Msa0314630 AT1G20130.2 31.217 189 105 6 26 194 310 493 2.11e-16 78.2
Msa0314630 AT2G24560.1 23.005 213 136 6 10 197 132 341 2.58e-16 77.0
Msa0314630 AT2G31550.1 25.616 203 123 7 19 196 1 200 3.43e-16 75.1
Msa0314630 AT1G23500.1 28.283 198 107 7 20 195 142 326 3.72e-16 76.6
Msa0314630 AT5G03810.1 26.267 217 136 6 7 202 128 341 3.92e-16 76.6
Msa0314630 AT5G55050.1 29.121 182 106 7 36 197 171 349 4.77e-16 76.6
Msa0314630 AT2G31540.1 24.413 213 133 7 10 197 133 342 7.06e-16 75.9
Msa0314630 AT5G45950.1 27.136 199 120 6 14 195 147 337 1.75e-15 74.7
Msa0314630 AT5G41890.1 27.039 233 129 10 24 223 148 372 7.92e-15 73.2
Msa0314630 AT1G20120.1 26.733 202 117 7 20 195 185 381 6.11e-14 70.5
Msa0314630 AT1G20132.1 29.534 193 110 6 24 194 175 363 1.38e-13 69.3
Msa0314630 AT5G45960.1 25.000 196 119 6 29 199 166 358 2.09e-13 68.9
Msa0314630 AT1G20135.1 27.711 166 97 4 44 195 164 320 2.57e-13 68.6
Msa0314630 AT1G73610.1 26.531 196 110 7 20 194 142 324 3.76e-13 68.2
Msa0314630 AT1G75880.1 25.714 210 129 7 10 195 150 356 5.37e-13 67.8
Msa0314630 AT1G71120.1 24.581 179 111 6 37 195 155 329 1.09e-12 66.6
Msa0314630 AT1G75900.1 27.429 175 104 5 41 195 174 345 2.80e-12 65.5
Msa0314630 AT3G14225.2 29.150 247 141 11 4 223 131 370 3.22e-12 65.5
Msa0314630 AT3G05180.1 26.214 206 107 5 36 203 165 363 8.28e-12 64.3
Msa0314630 AT3G14225.1 29.339 242 137 11 9 223 140 374 1.09e-11 63.9
Msa0314630 AT2G24560.2 23.377 154 108 4 10 156 132 282 1.29e-11 63.5
Msa0314630 AT1G54790.1 24.631 203 108 6 34 198 158 353 1.84e-11 63.2
Msa0314630 AT1G54790.3 24.631 203 108 6 34 198 159 354 2.34e-11 63.2
Msa0314630 AT4G16233.1 30.303 132 67 5 118 228 5 132 5.42e-11 58.9
Msa0314630 AT4G16230.1 33.010 103 67 2 24 124 143 245 7.73e-11 60.5
Msa0314630 AT1G75930.1 27.517 149 82 5 65 194 182 323 9.37e-11 61.2

Find 42 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTGTCTTGACTTAATTTA+TGG 0.077141 2_4:+10459674 None:intergenic
GCACTTATTTAGATAATTTA+TGG 0.141505 2_4:+10461817 None:intergenic
GGACAGTCTTTGTATGATTT+TGG 0.142004 2_4:-10460375 Msa0314630:intron
ACAAAGATAGATTTAGAATT+TGG 0.163926 2_4:+10460189 None:intergenic
TGCGACTTGTTGGGTAAAAT+TGG 0.181701 2_4:+10461741 None:intergenic
TCTAAGTTTGATAACAATTC+TGG 0.268560 2_4:+10461853 None:intergenic
AAAACGTAAACATTTCTATT+GGG 0.291264 2_4:+10459816 None:intergenic
CCAGTATTCTTCTTATATAA+AGG 0.291292 2_4:-10461681 Msa0314630:intron
CAGGGTGCTAATGTCGCTTT+GGG 0.330720 2_4:-10461908 Msa0314630:CDS
TAGATTTAGAATTTGGATAT+CGG 0.354893 2_4:+10460196 None:intergenic
GCAGGGTGCTAATGTCGCTT+TGG 0.358650 2_4:-10461909 Msa0314630:intron
GCGACTTGTTGGGTAAAATT+GGG 0.359646 2_4:+10461742 None:intergenic
CATGTTGGAATTGGCCTTAT+AGG 0.367186 2_4:-10460336 Msa0314630:CDS
AGAATTGTTATCAAACTTAG+AGG 0.376223 2_4:-10461851 Msa0314630:CDS
GTGTTATAGATGCGACTTGT+TGG 0.391018 2_4:+10461731 None:intergenic
CCTTTATATAAGAAGAATAC+TGG 0.401167 2_4:+10461681 None:intergenic
AAACTCGTGCATGTTGGAAT+TGG 0.402053 2_4:-10460345 Msa0314630:CDS
TGCTTGTATTATGTGAATAT+TGG 0.411716 2_4:-10461797 Msa0314630:CDS
TTGGGGCATGGAGTTGAATC+AGG 0.418156 2_4:+10459834 None:intergenic
TGTTGTCCAGTGAGGTCAGA+TGG 0.445100 2_4:-10459867 Msa0314630:CDS
TGTTATAGATGCGACTTGTT+GGG 0.450708 2_4:+10461732 None:intergenic
TCCATTGGATGGGTCGTCCC+TGG 0.471406 2_4:+10459720 None:intergenic
AATGTGGAGTGCAACCTATA+AGG 0.471432 2_4:+10460322 None:intergenic
TATGATAGTTCTTCTGCTCC+AGG 0.488195 2_4:-10459738 Msa0314630:CDS
AAACGTAAACATTTCTATTG+GGG 0.544555 2_4:+10459817 None:intergenic
CACATCCCATCTGACCTCAC+TGG 0.554719 2_4:+10459861 None:intergenic
CGACTTGTTGGGTAAAATTG+GGG 0.556831 2_4:+10461743 None:intergenic
GAGAAAGTTCACAGCTGATG+TGG 0.560323 2_4:+10459776 None:intergenic
GCAAGAAAACTCGTGCATGT+TGG 0.562092 2_4:-10460351 Msa0314630:CDS
ATTGCTACTCGAGGGACAGA+TGG 0.569780 2_4:-10460297 Msa0314630:CDS
TCTCAAACTTAGATCTCTAG+TGG 0.570387 2_4:-10460232 Msa0314630:CDS
TAAACATTTCTATTGGGGCA+TGG 0.572507 2_4:+10459822 None:intergenic
GTTGTCCAGTGAGGTCAGAT+GGG 0.580131 2_4:-10459866 Msa0314630:CDS
ATGATAGTTCTTCTGCTCCA+GGG 0.599199 2_4:-10459737 Msa0314630:CDS
CACATTCAATTGCTACTCGA+GGG 0.607858 2_4:-10460305 Msa0314630:CDS
CCACATTCAATTGCTACTCG+AGG 0.609254 2_4:-10460306 Msa0314630:CDS
AAGAACTTCAGCATATTGCT+CGG 0.615665 2_4:+10461709 None:intergenic
TCTGACCTCACTGGACAACA+AGG 0.616362 2_4:+10459870 None:intergenic
TCCAGGGACGACCCATCCAA+TGG 0.625845 2_4:-10459721 Msa0314630:CDS
CCTCGAGTAGCAATTGAATG+TGG 0.637737 2_4:+10460306 None:intergenic
AGAAAGTTCACAGCTGATGT+GGG 0.668712 2_4:+10459777 None:intergenic
GTGCTCCTTGTTGTCCAGTG+AGG 0.670118 2_4:-10459875 Msa0314630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATAAAATTTTATAAAT+TGG + chr2_4:10461432-10461451 None:intergenic 0.0%
!! ATTTATTAAATGAAATACAT+CGG + chr2_4:10459769-10459788 None:intergenic 10.0%
!!! GAATATATATAATATTTTTG+AGG + chr2_4:10460291-10460310 None:intergenic 10.0%
!!! CAAATATAATTTTTTTTTTG+AGG + chr2_4:10461457-10461476 None:intergenic 10.0%
!! AAAAAAAATTATATTTGTCA+TGG - chr2_4:10461460-10461479 Msa0314630:intron 10.0%
!! GATGTATTTCATTTAATAAA+TGG - chr2_4:10459768-10459787 Msa0314630:CDS 15.0%
!! TAACCTTAGATTTAATATAA+TGG - chr2_4:10459804-10459823 Msa0314630:CDS 15.0%
!! AAAGTAGTAAAAAATAGAAT+GGG + chr2_4:10460188-10460207 None:intergenic 15.0%
!! AAAAGTAGTAAAAAATAGAA+TGG + chr2_4:10460189-10460208 None:intergenic 15.0%
!!! AAAATTGATTTTTTTTTACG+AGG + chr2_4:10461174-10461193 None:intergenic 15.0%
!!! AGAATTTTATGTTTTGTAAA+GGG - chr2_4:10461247-10461266 Msa0314630:intron 15.0%
!!! GCTTTCTTTAAAAAAAAAAA+TGG - chr2_4:10461270-10461289 Msa0314630:intron 15.0%
!! TAGCCATTATATTAAATCTA+AGG + chr2_4:10459810-10459829 None:intergenic 20.0%
!! TTAGATTTAATATAATGGCT+AGG - chr2_4:10459809-10459828 Msa0314630:CDS 20.0%
!! TAGATTTAATATAATGGCTA+GGG - chr2_4:10459810-10459829 Msa0314630:CDS 20.0%
!!! AATAATTGTCTGAGATTAAA+AGG - chr2_4:10460128-10460147 Msa0314630:intron 20.0%
!! AAGTAGTAAAAAATAGAATG+GGG + chr2_4:10460187-10460206 None:intergenic 20.0%
!! GCACTTATTTAGATAATTTA+TGG + chr2_4:10460458-10460477 None:intergenic 20.0%
!!! TTTTAGAGCTTAAAATGTAT+TGG + chr2_4:10461042-10461061 None:intergenic 20.0%
!!! CAGAATTTTATGTTTTGTAA+AGG - chr2_4:10461246-10461265 Msa0314630:intron 20.0%
!!! GAATTTTATGTTTTGTAAAG+GGG - chr2_4:10461248-10461267 Msa0314630:intron 20.0%
!!! ATTTTATAAATTGGTGTGTT+TGG + chr2_4:10461423-10461442 None:intergenic 20.0%
!!! ACAAATACATGATTTTCTTA+TGG + chr2_4:10461514-10461533 None:intergenic 20.0%
!!! AACTATTCTGATAATGATTT+CGG - chr2_4:10461624-10461643 Msa0314630:intron 20.0%
!! TGAAGTTTGAAAATGTAATA+GGG - chr2_4:10461826-10461845 Msa0314630:CDS 20.0%
!!! TTTTTCAACAATGATTTGAA+TGG - chr2_4:10461863-10461882 Msa0314630:CDS 20.0%
!!! TAGATTTAGAATTTGGATAT+CGG + chr2_4:10462079-10462098 None:intergenic 20.0%
!!! ACAAAGATAGATTTAGAATT+TGG + chr2_4:10462086-10462105 None:intergenic 20.0%
!! GATGTATCTAATTCAAAAAT+TGG + chr2_4:10462245-10462264 None:intergenic 20.0%
!!! CAATTGATAATGTATGATAT+AGG - chr2_4:10462313-10462332 Msa0314630:intron 20.0%
!!! ACTATGATATTTTGTATTTC+AGG - chr2_4:10462360-10462379 Msa0314630:intron 20.0%
!! AAAACGTAAACATTTCTATT+GGG + chr2_4:10462459-10462478 None:intergenic 20.0%
!! AAAAACGTAAACATTTCTAT+TGG + chr2_4:10462460-10462479 None:intergenic 20.0%
!!! AATGTTTACGTTTTTTATGA+TGG - chr2_4:10462465-10462484 Msa0314630:intron 20.0%
! GAGAAAAATTGAGATAAAAG+TGG - chr2_4:10459842-10459861 Msa0314630:CDS 25.0%
! CTATATATGCAATATTTGCA+GGG - chr2_4:10460346-10460365 Msa0314630:CDS 25.0%
!! TCTAAGTTTGATAACAATTC+TGG + chr2_4:10460422-10460441 None:intergenic 25.0%
! AGAATTGTTATCAAACTTAG+AGG - chr2_4:10460421-10460440 Msa0314630:intron 25.0%
! TGCTTGTATTATGTGAATAT+TGG - chr2_4:10460475-10460494 Msa0314630:intron 25.0%
! CCTTTATATAAGAAGAATAC+TGG + chr2_4:10460594-10460613 None:intergenic 25.0%
! CCAGTATTCTTCTTATATAA+AGG - chr2_4:10460591-10460610 Msa0314630:intron 25.0%
! CTACATATCAACCATAAAAT+TGG + chr2_4:10460818-10460837 None:intergenic 25.0%
! TCATGGATCCTAAATATATT+AGG - chr2_4:10461308-10461327 Msa0314630:intron 25.0%
! GAGGTAAACCTAATATATTT+AGG + chr2_4:10461319-10461338 None:intergenic 25.0%
!! AATTTAGTTTACTACAACAG+AGG + chr2_4:10461338-10461357 None:intergenic 25.0%
! AACTTATCGATCATATCATT+TGG - chr2_4:10461358-10461377 Msa0314630:intron 25.0%
! ATCATTTGGATAAATGTTCA+TGG - chr2_4:10461372-10461391 Msa0314630:intron 25.0%
!! CATTGCATCAATAATTTTTG+TGG + chr2_4:10461487-10461506 None:intergenic 25.0%
! GTGAAGTTTGAAAATGTAAT+AGG - chr2_4:10461825-10461844 Msa0314630:CDS 25.0%
!!! ATTTGAATGGCATTTTTTTG+TGG - chr2_4:10461876-10461895 Msa0314630:CDS 25.0%
! AAACGTAAACATTTCTATTG+GGG + chr2_4:10462458-10462477 None:intergenic 25.0%
!!! TTGTTTTATATCCATTGGAT+GGG + chr2_4:10462565-10462584 None:intergenic 25.0%
!!! ATTATGAGTTTTTTGCGTGT+GGG - chr2_4:10459717-10459736 Msa0314630:CDS 30.0%
! CACACATTTGATGACATTAA+GGG + chr2_4:10459885-10459904 None:intergenic 30.0%
! ACACACATTTGATGACATTA+AGG + chr2_4:10459886-10459905 None:intergenic 30.0%
ATATGCAAACATTAGTGACT+CGG - chr2_4:10460000-10460019 Msa0314630:intron 30.0%
GCTATATATGCAATATTTGC+AGG - chr2_4:10460345-10460364 Msa0314630:CDS 30.0%
! TTCTGGCAAAGATGATTTTA+TGG + chr2_4:10460405-10460424 None:intergenic 30.0%
! AACTTCTGCTACCAATTTTA+TGG - chr2_4:10460804-10460823 Msa0314630:intron 30.0%
CATAATATAGTACCTAACAG+AGG - chr2_4:10460974-10460993 Msa0314630:intron 30.0%
!! GATAAATGTTCATGGTTTTG+TGG - chr2_4:10461380-10461399 Msa0314630:intron 30.0%
GTAAAAACTTGTGTTATGGA+GGG + chr2_4:10461571-10461590 None:intergenic 30.0%
TGTAAAAACTTGTGTTATGG+AGG + chr2_4:10461572-10461591 None:intergenic 30.0%
CTGTGTAAAAACTTGTGTTA+TGG + chr2_4:10461575-10461594 None:intergenic 30.0%
CATTATACAAATGTTGTGGA+CGG + chr2_4:10461790-10461809 None:intergenic 30.0%
CATGCATTATACAAATGTTG+TGG + chr2_4:10461794-10461813 None:intergenic 30.0%
! GACAGTCTTTGTATGATTTT+GGG - chr2_4:10461898-10461917 Msa0314630:CDS 30.0%
! ACAGTCTTTGTATGATTTTG+GGG - chr2_4:10461899-10461918 Msa0314630:CDS 30.0%
ATCTTTGTAAATAGTACTGC+TGG - chr2_4:10462098-10462117 Msa0314630:intron 30.0%
TGTTGACAAAACTTTCTCTT+AGG - chr2_4:10462271-10462290 Msa0314630:intron 30.0%
!! GTTGTTTTATATCCATTGGA+TGG + chr2_4:10462566-10462585 None:intergenic 30.0%
!! GCAAGTTGTTTTATATCCAT+TGG + chr2_4:10462570-10462589 None:intergenic 30.0%
!!! GATTATGAGTTTTTTGCGTG+TGG - chr2_4:10459716-10459735 Msa0314630:CDS 35.0%
!! TGAGATAAAAGTGGATTGAG+AGG - chr2_4:10459851-10459870 Msa0314630:CDS 35.0%
!! AAAAGTGGATTGAGAGGATT+TGG - chr2_4:10459857-10459876 Msa0314630:CDS 35.0%
! AGCTAACATTTTACCGTTGT+AGG - chr2_4:10459944-10459963 Msa0314630:intron 35.0%
CACTAATGTTTGCATATGGT+GGG + chr2_4:10459997-10460016 None:intergenic 35.0%
TCACTAATGTTTGCATATGG+TGG + chr2_4:10459998-10460017 None:intergenic 35.0%
GAGTCACTAATGTTTGCATA+TGG + chr2_4:10460001-10460020 None:intergenic 35.0%
ACACAATACCATATGTTTGG+CGG + chr2_4:10460083-10460102 None:intergenic 35.0%
CACACACAATACCATATGTT+TGG + chr2_4:10460086-10460105 None:intergenic 35.0%
! AGAATGTTTGTACGGTGTTA+GGG + chr2_4:10460232-10460251 None:intergenic 35.0%
AAGAATGTTTGTACGGTGTT+AGG + chr2_4:10460233-10460252 None:intergenic 35.0%
CTTGAGGAAGAATGTTTGTA+CGG + chr2_4:10460240-10460259 None:intergenic 35.0%
! TGTTATAGATGCGACTTGTT+GGG + chr2_4:10460543-10460562 None:intergenic 35.0%
AAGAACTTCAGCATATTGCT+CGG + chr2_4:10460566-10460585 None:intergenic 35.0%
GATAAGCCTCAAGGTTTAAA+GGG + chr2_4:10460856-10460875 None:intergenic 35.0%
AATATAGTACCTAACAGAGG+TGG - chr2_4:10460977-10460996 Msa0314630:intron 35.0%
!!! ATGTTCATGGTTTTGTGGTA+TGG - chr2_4:10461385-10461404 Msa0314630:intron 35.0%
CAAAATATACCCCAGTTTCT+CGG - chr2_4:10461657-10461676 Msa0314630:intron 35.0%
CATGAATGCTTCTTCAATGT+CGG + chr2_4:10461745-10461764 None:intergenic 35.0%
! GGACAGTCTTTGTATGATTT+TGG - chr2_4:10461897-10461916 Msa0314630:CDS 35.0%
TCTCAAACTTAGATCTCTAG+TGG - chr2_4:10462040-10462059 Msa0314630:intron 35.0%
AAACTTTCTCTTAGGATCGA+AGG - chr2_4:10462279-10462298 Msa0314630:intron 35.0%
AACTTTCTCTTAGGATCGAA+GGG - chr2_4:10462280-10462299 Msa0314630:intron 35.0%
TAAACATTTCTATTGGGGCA+TGG + chr2_4:10462453-10462472 None:intergenic 35.0%
ATGGCATATGTACGTGTTTG+AGG - chr2_4:10459686-10459705 Msa0314630:CDS 40.0%
TCCAAGCATTTCACCTACAA+CGG + chr2_4:10459960-10459979 None:intergenic 40.0%
ATAGAATGGGGTGAGAAAGA+TGG + chr2_4:10460175-10460194 None:intergenic 40.0%
CAAACATTCTTCCTCAAGCA+CGG - chr2_4:10460242-10460261 Msa0314630:CDS 40.0%
AAACATTCTTCCTCAAGCAC+GGG - chr2_4:10460243-10460262 Msa0314630:CDS 40.0%
!! CGACTTGTTGGGTAAAATTG+GGG + chr2_4:10460532-10460551 None:intergenic 40.0%
!! GCGACTTGTTGGGTAAAATT+GGG + chr2_4:10460533-10460552 None:intergenic 40.0%
!! TGCGACTTGTTGGGTAAAAT+TGG + chr2_4:10460534-10460553 None:intergenic 40.0%
GTGTTATAGATGCGACTTGT+TGG + chr2_4:10460544-10460563 None:intergenic 40.0%
! TTGATTCGCTCTTAATACCG+TGG - chr2_4:10460777-10460796 Msa0314630:intron 40.0%
TGATTCGCTCTTAATACCGT+GGG - chr2_4:10460778-10460797 Msa0314630:intron 40.0%
TAACCACCCTTTAAACCTTG+AGG - chr2_4:10460847-10460866 Msa0314630:intron 40.0%
GGATAAGCCTCAAGGTTTAA+AGG + chr2_4:10460857-10460876 None:intergenic 40.0%
TCAGATAAGGATAAGCCTCA+AGG + chr2_4:10460865-10460884 None:intergenic 40.0%
ATCCTTATCTGATGAGCCTT+CGG - chr2_4:10460873-10460892 Msa0314630:intron 40.0%
TGATATTGTCCACCTCTGTT+AGG + chr2_4:10460989-10461008 None:intergenic 40.0%
ACAGAGGTGGACAATATCAT+TGG - chr2_4:10460990-10461009 Msa0314630:intron 40.0%
CAGAGGTGGACAATATCATT+GGG - chr2_4:10460991-10461010 Msa0314630:intron 40.0%
AGAGGTGGACAATATCATTG+GGG - chr2_4:10460992-10461011 Msa0314630:intron 40.0%
TGGAGTCTACTAGACAAAGT+CGG - chr2_4:10461015-10461034 Msa0314630:intron 40.0%
GGATAAGTGTGACATCATCA+TGG - chr2_4:10461291-10461310 Msa0314630:intron 40.0%
CGGTCAAACAGTCAAAAATG+TGG + chr2_4:10461725-10461744 None:intergenic 40.0%
AAACTCGTGCATGTTGGAAT+TGG - chr2_4:10461927-10461946 Msa0314630:intron 40.0%
!! CATGTTGGAATTGGCCTTAT+AGG - chr2_4:10461936-10461955 Msa0314630:intron 40.0%
AATGTGGAGTGCAACCTATA+AGG + chr2_4:10461953-10461972 None:intergenic 40.0%
CACATTCAATTGCTACTCGA+GGG - chr2_4:10461967-10461986 Msa0314630:intron 40.0%
CTTACCAAGTGATTCGTCTA+TGG + chr2_4:10462126-10462145 None:intergenic 40.0%
AGAAAGTTCACAGCTGATGT+GGG + chr2_4:10462498-10462517 None:intergenic 40.0%
TATGATAGTTCTTCTGCTCC+AGG - chr2_4:10462534-10462553 Msa0314630:intron 40.0%
ATGATAGTTCTTCTGCTCCA+GGG - chr2_4:10462535-10462554 Msa0314630:intron 40.0%
ACCGTTGTAGGTGAAATGCT+TGG - chr2_4:10459956-10459975 Msa0314630:intron 45.0%
CGTGGTGTAAACTATGCATC+TGG - chr2_4:10460034-10460053 Msa0314630:intron 45.0%
AACTATGCATCTGGTGCATC+TGG - chr2_4:10460043-10460062 Msa0314630:intron 45.0%
TATAAAGTTGCCCGTGCTTG+AGG + chr2_4:10460256-10460275 None:intergenic 45.0%
AAGCCTCAAGGTTTAAAGGG+TGG + chr2_4:10460853-10460872 None:intergenic 45.0%
TTATGCGAGTTAGACTCCGA+AGG + chr2_4:10460892-10460911 None:intergenic 45.0%
TGTGGACGGTTTCTTGTTGA+AGG + chr2_4:10461776-10461795 None:intergenic 45.0%
GCAAGAAAACTCGTGCATGT+TGG - chr2_4:10461921-10461940 Msa0314630:intron 45.0%
CCTCGAGTAGCAATTGAATG+TGG + chr2_4:10461969-10461988 None:intergenic 45.0%
CCACATTCAATTGCTACTCG+AGG - chr2_4:10461966-10461985 Msa0314630:intron 45.0%
GAGAAAGTTCACAGCTGATG+TGG + chr2_4:10462499-10462518 None:intergenic 45.0%
!!! AATATATATAATATTTTTGA+GGG + chr2_4:10460290-10460309 None:intergenic 5.0%
!!! AAATATAATTTTTTTTTTGA+GGG + chr2_4:10461456-10461475 None:intergenic 5.0%
! GTTTGTACGGTGTTAGGGAG+AGG + chr2_4:10460227-10460246 None:intergenic 50.0%
!! CAGGGTGCTAATGTCGCTTT+GGG - chr2_4:10460364-10460383 Msa0314630:CDS 50.0%
GGTAGCAGAAGTTGAACCCA+CGG + chr2_4:10460797-10460816 None:intergenic 50.0%
CTCCGAAGGCTCATCAGATA+AGG + chr2_4:10460878-10460897 None:intergenic 50.0%
GGTGGACAATATCATTGGGG+TGG - chr2_4:10460995-10461014 Msa0314630:intron 50.0%
ACCTTGCTGACCGAGAAACT+GGG + chr2_4:10461670-10461689 None:intergenic 50.0%
AACCTTGCTGACCGAGAAAC+TGG + chr2_4:10461671-10461690 None:intergenic 50.0%
AGTTTCTCGGTCAGCAAGGT+TGG - chr2_4:10461670-10461689 Msa0314630:intron 50.0%
ATTGCTACTCGAGGGACAGA+TGG - chr2_4:10461975-10461994 Msa0314630:intron 50.0%
GGCTCCATAGACGAATCACT+TGG - chr2_4:10462119-10462138 Msa0314630:intron 50.0%
TCTGACCTCACTGGACAACA+AGG + chr2_4:10462405-10462424 None:intergenic 50.0%
TGTTGTCCAGTGAGGTCAGA+TGG - chr2_4:10462405-10462424 Msa0314630:intron 50.0%
GTTGTCCAGTGAGGTCAGAT+GGG - chr2_4:10462406-10462425 Msa0314630:intron 50.0%
TTGGGGCATGGAGTTGAATC+AGG + chr2_4:10462441-10462460 None:intergenic 50.0%
GACTCGGACGACATACTTCG+TGG - chr2_4:10460016-10460035 Msa0314630:intron 55.0%
! TGGTGCATCTGGAATCCGCT+CGG - chr2_4:10460054-10460073 Msa0314630:intron 55.0%
TATGTTTGGCGGTCTCCGAG+CGG + chr2_4:10460072-10460091 None:intergenic 55.0%
CTCGGAGACCGCCAAACATA+TGG - chr2_4:10460072-10460091 Msa0314630:intron 55.0%
! GCAGGGTGCTAATGTCGCTT+TGG - chr2_4:10460363-10460382 Msa0314630:CDS 55.0%
CCTTGCTGACCGAGAAACTG+GGG + chr2_4:10461669-10461688 None:intergenic 55.0%
! GTGCTCCTTGTTGTCCAGTG+AGG - chr2_4:10462397-10462416 Msa0314630:intron 55.0%
CACATCCCATCTGACCTCAC+TGG + chr2_4:10462414-10462433 None:intergenic 55.0%
CCCCAGTTTCTCGGTCAGCA+AGG - chr2_4:10461666-10461685 Msa0314630:intron 60.0%
TCCAGGGACGACCCATCCAA+TGG - chr2_4:10462551-10462570 Msa0314630:intron 60.0%
TCCATTGGATGGGTCGTCCC+TGG + chr2_4:10462555-10462574 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 10459686 10462608 10459686 ID=Msa0314630;Name=Msa0314630
chr2_4 mRNA 10459686 10462608 10459686 ID=Msa0314630-mRNA-1;Parent=Msa0314630;Name=Msa0314630-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|0.75|0.33|0.5|4|0|229
chr2_4 exon 10462600 10462608 10462600 ID=Msa0314630-mRNA-1:exon:4009;Parent=Msa0314630-mRNA-1
chr2_4 exon 10461682 10461927 10461682 ID=Msa0314630-mRNA-1:exon:4008;Parent=Msa0314630-mRNA-1
chr2_4 exon 10460184 10460391 10460184 ID=Msa0314630-mRNA-1:exon:4007;Parent=Msa0314630-mRNA-1
chr2_4 exon 10459686 10459912 10459686 ID=Msa0314630-mRNA-1:exon:4006;Parent=Msa0314630-mRNA-1
chr2_4 CDS 10462600 10462608 10462600 ID=Msa0314630-mRNA-1:cds;Parent=Msa0314630-mRNA-1
chr2_4 CDS 10461682 10461927 10461682 ID=Msa0314630-mRNA-1:cds;Parent=Msa0314630-mRNA-1
chr2_4 CDS 10460184 10460391 10460184 ID=Msa0314630-mRNA-1:cds;Parent=Msa0314630-mRNA-1
chr2_4 CDS 10459686 10459912 10459686 ID=Msa0314630-mRNA-1:cds;Parent=Msa0314630-mRNA-1
Gene Sequence

>Msa0314630

ATGGCATATGGTGCTAATGTCGCTTTGGGATTACAAATATTACACCATAAAATCATCTTTGCCAGAATTGTTATCAAACTTAGAGGTTTGCGAAATGCCATAAATTATCTAAATAAGTGCTTGTATTATGTGAATATTGGCAGCAACGATTACATAAACAACTACTACTTGCCCCAATTTTACCCAACAAGTCGCATCTATAACACCGAGCAATATGCTGAAGTTCTTATTAGCCAGTATTCTTCTTATATAAAGTCTTTGTATGATTTTGGGGCAAGAAAACTCGTGCATGTTGGAATTGGCCTTATAGGTTGCACTCCACATTCAATTGCTACTCGAGGGACAGATGGATCATGTGTTGAAGAGCAGAATGCAGCATCATTAATATTTAGTCTCAAACTTAGATCTCTAGTGGATCAATTAAATATCCGATATCCAAATTCTAAATCTATCTTTGTAAATAGTTTTACGAATATAAGTGCTCCTTGTTGTCCAGTGAGGTCAGATGGGATGTGCGTTCCTGATTCAACTCCATGCCCCAATAGAAATGTTTACGTTTTTTATGATGGATTTCATCCCACATCAGCTGTGAACTTTCTCACTGCATTAACATCGTATGATAGTTCTTCTGCTCCAGGGACGACCCATCCAATGGATATAAAACAACTTGCTCAATATTCCATAAATTAA
Protein sequence

>Msa0314630

MAYGANVALGLQILHHKIIFARIVIKLRGLRNAINYLNKCLYYVNIGSNDYINNYYLPQFYPTSRIYNTEQYAEVLISQYSSYIKSLYDFGARKLVHVGIGLIGCTPHSIATRGTDGSCVEEQNAASLIFSLKLRSLVDQLNIRYPNSKSIFVNSFTNISAPCCPVRSDGMCVPDSTPCPNRNVYVFYDGFHPTSAVNFLTALTSYDSSSAPGTTHPMDIKQLAQYSIN*