AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1174460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1174460 MtrunA17_Chr4g0069691 93.886 229 13 1 1 229 1 228 6.13e-121 343
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1174460 AT3G43520.1 69.748 119 35 1 111 229 123 240 4.32e-51 166
Msa1174460 AT2G26240.1 63.810 105 38 0 124 228 4 108 3.74e-38 129

Find 106 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCCTCTTGTTATGAAATT+TGG 0.026882 8_1:+5556222 Msa1174460:three_prime_UTR
CCCCTCTTGTTATGAAATTT+GGG 0.209009 8_1:+5556223 Msa1174460:three_prime_UTR
TAGACATTTGTTGTAAAATA+AGG 0.232439 8_1:+5556286 Msa1174460:three_prime_UTR
GGCGCCGTGGTTTCTGTATT+GGG 0.247815 8_1:-5553455 None:intergenic
TAGGTCAAATTGACTGGTTT+TGG 0.265563 8_1:-5553708 None:intergenic
TGGCGCCGTGGTTTCTGTAT+TGG 0.272823 8_1:-5553456 None:intergenic
TGAACCTATGCATATGCTTT+TGG 0.274183 8_1:+5556377 Msa1174460:three_prime_UTR
GAAATCAGGGAAGGTCTTTC+CGG 0.282767 8_1:+5556077 Msa1174460:CDS
CTCCTCTCAATCTGTTTCGC+TGG 0.292658 8_1:-5556440 None:intergenic
GGAGTGTGGGATCGGGTTGT+AGG 0.296999 8_1:-5553587 None:intergenic
TTGTATTCAGCAAAGTTTAC+AGG 0.324932 8_1:-5556199 None:intergenic
GGTTCTCGTTTCAAGAAATC+AGG 0.336113 8_1:+5556063 Msa1174460:CDS
AAAGGAAACGGAGACGATTT+CGG 0.340053 8_1:+5553773 Msa1174460:CDS
TTGACAGCGGGTGGTGTAAT+GGG 0.345679 8_1:+5555078 Msa1174460:intron
TGATGCAAGAACAGGTCTTC+CGG 0.347788 8_1:-5555184 None:intergenic
TTTGACAGCGGGTGGTGTAA+TGG 0.350276 8_1:+5555077 Msa1174460:intron
TGGCAGCCAGAAGTCACTTT+TGG 0.366373 8_1:+5555113 Msa1174460:CDS
GGTGGTGGTGGTGAAGGAGG+AGG 0.373824 8_1:+5553806 Msa1174460:CDS
GGTGGTGAAGGAGGAGGCGG+AGG 0.375033 8_1:+5553812 Msa1174460:CDS
ATGGTTTATGTTTGTCATAA+AGG 0.380251 8_1:+5556263 Msa1174460:three_prime_UTR
TGCTGCACTTCTTGTGGTGA+TGG 0.380439 8_1:+5556041 Msa1174460:CDS
GCCGCCGGTAATGTCTAAAT+CGG 0.388850 8_1:-5553738 None:intergenic
GGTGGTGGTGAAGGAGGAGG+CGG 0.392210 8_1:+5553809 Msa1174460:CDS
TGCATAGGTTCAAACACAAC+CGG 0.412204 8_1:-5556366 None:intergenic
CCGCCGGTAATGTCTAAATC+GGG 0.413389 8_1:-5553737 None:intergenic
CCCCAGCCAAAAGTGACTTC+TGG 0.415128 8_1:-5555119 None:intergenic
TCACTGGAAGAAGATTCTTT+TGG 0.418232 8_1:-5553476 None:intergenic
TAGTGGTGGGAGTGTGGGAT+CGG 0.421797 8_1:-5553595 None:intergenic
CCATTGACGAAAGAAGGTTC+TGG 0.425009 8_1:-5553505 None:intergenic
GACGATTTCGGCGGAGGAGG+TGG 0.426057 8_1:+5553785 Msa1174460:CDS
CTTGCATCATCTGTGGGCCT+TGG 0.426584 8_1:+5555198 Msa1174460:CDS
CCAGAACCTTCTTTCGTCAA+TGG 0.430434 8_1:+5553505 Msa1174460:exon
GATGCAAGAACAGGTCTTCC+GGG 0.430490 8_1:-5555183 None:intergenic
GAGAAATAGTGGTGGGAGTG+TGG 0.440353 8_1:-5553601 None:intergenic
GCTGCACTTCTTGTGGTGAT+GGG 0.443502 8_1:+5556042 Msa1174460:CDS
AGCGAGTTGAGAATGTGAAT+TGG 0.445867 8_1:-5553669 None:intergenic
GGCGGAGGAGGTGGTGGTGG+TGG 0.449465 8_1:+5553794 Msa1174460:CDS
TCCTCTCAATCTGTTTCGCT+GGG 0.449558 8_1:-5556439 None:intergenic
AGTGGTGGGAGTGTGGGATC+GGG 0.454693 8_1:-5553594 None:intergenic
TTTCTGCCATTGACGAAAGA+AGG 0.457559 8_1:-5553511 None:intergenic
TTCGGCGGAGGAGGTGGTGG+TGG 0.461763 8_1:+5553791 Msa1174460:CDS
GATTTCGGCGGAGGAGGTGG+TGG 0.468875 8_1:+5553788 Msa1174460:CDS
GACAAGTAAGGGAAAGAAGC+AGG 0.472035 8_1:-5553641 None:intergenic
GGCACTTAGGTCAAATTGAC+TGG 0.474158 8_1:-5553714 None:intergenic
ACCTGTTCTTGCATCATCTG+TGG 0.483230 8_1:+5555191 Msa1174460:CDS
AACGGAGACGATTTCGGCGG+AGG 0.486679 8_1:+5553779 Msa1174460:CDS
AGAAGCAGGAGAAGCAAGGA+AGG 0.489884 8_1:-5553627 None:intergenic
CCCACAGATGATGCAAGAAC+AGG 0.490656 8_1:-5555192 None:intergenic
CTAGGTTATGCTATTCTCGT+TGG 0.491350 8_1:+5553914 Msa1174460:CDS
AAGGAAGGAGCGAGAAATAG+TGG 0.498256 8_1:-5553612 None:intergenic
AAGGATCTGACGGTGATAAG+AGG 0.499849 8_1:+5553858 Msa1174460:CDS
AACAGGTCTTCCGGGTAGCT+CGG 0.505808 8_1:-5555175 None:intergenic
TTTGATATCACCGCCGCCGC+CGG 0.506217 8_1:-5553753 None:intergenic
TTAGACATTACCGGCGGCGG+CGG 0.514783 8_1:+5553743 Msa1174460:CDS
GGTTATGCTATTCTCGTTGG+AGG 0.515433 8_1:+5553917 Msa1174460:CDS
GATTGAGAGGAGTAAACATA+AGG 0.520080 8_1:+5556451 Msa1174460:three_prime_UTR
CCTGTTCTTGCATCATCTGT+GGG 0.521720 8_1:+5555192 Msa1174460:CDS
GGAGACGATTTCGGCGGAGG+AGG 0.526381 8_1:+5553782 Msa1174460:CDS
GGGTTTGTGCTGTAGACCCA+TGG 0.529398 8_1:+5556244 Msa1174460:three_prime_UTR
GAGTGTGGGATCGGGTTGTA+GGG 0.536706 8_1:-5553586 None:intergenic
GGCGGTGATATCAAAGGAAA+CGG 0.540755 8_1:+5553761 Msa1174460:CDS
GATAGTAACAAAGGCGAGGA+AGG 0.548487 8_1:+5553839 Msa1174460:CDS
CATATCTGCTGCACTTCTTG+TGG 0.550309 8_1:+5556035 Msa1174460:CDS
CCCTCTTGTTATGAAATTTG+GGG 0.553952 8_1:+5556224 Msa1174460:three_prime_UTR
AAGGAGCGAGAAATAGTGGT+GGG 0.557007 8_1:-5553608 None:intergenic
TCAGGGAAGGTCTTTCCGGC+AGG 0.561022 8_1:+5556081 Msa1174460:CDS
CATTTGTTGTAAAATAAGGA+TGG 0.562464 8_1:+5556290 Msa1174460:three_prime_UTR
CTTGTGTCGTTCATAATGAC+CGG 0.562716 8_1:+5556114 Msa1174460:CDS
ACAAGTGACACAACACCTGC+CGG 0.564042 8_1:-5556096 None:intergenic
AGAAATAGTGGTGGGAGTGT+GGG 0.564262 8_1:-5553600 None:intergenic
GGTTGTAGGGCGAAGAGAGA+GGG 0.564448 8_1:-5553573 None:intergenic
ATGACCGGTGGTTACTTGCA+TGG 0.564829 8_1:+5556129 Msa1174460:CDS
TCACACTAGAATGTAAATAA+CGG 0.564961 8_1:+5556165 Msa1174460:exon
ACCCAGCGAAACAGATTGAG+AGG 0.567492 8_1:+5556438 Msa1174460:three_prime_UTR
GTAGGGCGAAGAGAGAGGGA+AGG 0.571612 8_1:-5553569 None:intergenic
ACCCCAAATTTCATAACAAG+AGG 0.577194 8_1:-5556225 None:intergenic
ATGTCGCAGAAATTAACTCT+AGG 0.581649 8_1:+5553896 Msa1174460:CDS
CCCCAAATTTCATAACAAGA+GGG 0.582804 8_1:-5556224 None:intergenic
TTTATGACAAACATAAACCA+TGG 0.585195 8_1:-5556261 None:intergenic
TGACGGTGATAAGAGGAAGA+TGG 0.587068 8_1:+5553865 Msa1174460:CDS
CCCAAATTTCATAACAAGAG+GGG 0.587441 8_1:-5556223 None:intergenic
GAATTGGAAAGAGACAAGTA+AGG 0.589380 8_1:-5553653 None:intergenic
GATTTAGACATTACCGGCGG+CGG 0.591983 8_1:+5553740 Msa1174460:CDS
TTATGACAAACATAAACCAT+GGG 0.603493 8_1:-5556260 None:intergenic
CGCCGGTAATGTCTAAATCG+GGG 0.604362 8_1:-5553736 None:intergenic
GGAAAGAAGCAGGAGAAGCA+AGG 0.608774 8_1:-5553631 None:intergenic
AATTGGAAAGAGACAAGTAA+GGG 0.611539 8_1:-5553652 None:intergenic
AGTTCCAAAAGCATATGCAT+AGG 0.623095 8_1:-5556381 None:intergenic
GGCGGCGGCGGTGATATCAA+AGG 0.624581 8_1:+5553755 Msa1174460:CDS
GTTCTCGTTTCAAGAAATCA+GGG 0.628947 8_1:+5556064 Msa1174460:CDS
TCGTTTCAAGAAATCAGGGA+AGG 0.630412 8_1:+5556068 Msa1174460:CDS
GCCGGTAATGTCTAAATCGG+GGG 0.634119 8_1:-5553735 None:intergenic
GTAATGGGCTTTCTGAAGAG+TGG 0.639725 8_1:+5555093 Msa1174460:CDS
AATTCCATGCAAGTAACCAC+CGG 0.644415 8_1:-5556133 None:intergenic
AAAGGCGAGGAAGGATCTGA+CGG 0.645896 8_1:+5553848 Msa1174460:CDS
AGACGATAGTAACAAAGGCG+AGG 0.650186 8_1:+5553835 Msa1174460:CDS
GAAGGAGCGAGAAATAGTGG+TGG 0.655482 8_1:-5553609 None:intergenic
CCCGATTTAGACATTACCGG+CGG 0.658939 8_1:+5553737 Msa1174460:CDS
GCATAGGTTCAAACACAACC+GGG 0.658942 8_1:-5556365 None:intergenic
GGAGGAGACGATAGTAACAA+AGG 0.659434 8_1:+5553830 Msa1174460:CDS
GGGTTGTAGGGCGAAGAGAG+AGG 0.659984 8_1:-5553574 None:intergenic
GTGTCGTTCATAATGACCGG+TGG 0.667012 8_1:+5556117 Msa1174460:CDS
CCTTACAAAAGACGAAAGAG+TGG 0.672058 8_1:+5553432 Msa1174460:five_prime_UTR
GTCAATGGCAGAAACTGCAG+CGG 0.675091 8_1:+5553520 Msa1174460:exon
GTGGCCCAATACAGAAACCA+CGG 0.696688 8_1:+5553451 Msa1174460:five_prime_UTR
GGAAACGGAGACGATTTCGG+CGG 0.728758 8_1:+5553776 Msa1174460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAAAATTATTTAACA+TGG + chr8_1:5555378-5555397 Msa1174460:intron 10.0%
!!! TTTATTTTGGAATATTATTT+TGG + chr8_1:5555839-5555858 Msa1174460:intron 10.0%
!! TTTCTTATGTTAGTTATTTA+AGG + chr8_1:5554123-5554142 Msa1174460:intron 15.0%
!! TCTACATAATGATATTTATT+TGG + chr8_1:5555052-5555071 Msa1174460:intron 15.0%
!! CTACATAATGATATTTATTT+GGG + chr8_1:5555053-5555072 Msa1174460:intron 15.0%
!! AAAAAATTATTTAACATGGA+TGG + chr8_1:5555382-5555401 Msa1174460:intron 15.0%
!! TAATATTCCAAAATAAATTC+AGG - chr8_1:5555836-5555855 None:intergenic 15.0%
!! GAAATAAAATCTCACAAAAA+CGG + chr8_1:5554528-5554547 Msa1174460:intron 20.0%
!! ATAGATAAGTGTTTATAAGT+GGG - chr8_1:5554574-5554593 None:intergenic 20.0%
!! ATAAACTGGAATAAATTGTA+TGG + chr8_1:5554732-5554751 Msa1174460:intron 20.0%
!! GATATTTGACAACATTAAAT+TGG + chr8_1:5554858-5554877 Msa1174460:intron 20.0%
!!! AGTTTTTTATCCATACTTTA+AGG + chr8_1:5555570-5555589 Msa1174460:intron 20.0%
!!! TTCTAAAAAACCTTAAAGTA+TGG - chr8_1:5555583-5555602 None:intergenic 20.0%
!!! TAGACATTTGTTGTAAAATA+AGG + chr8_1:5556286-5556305 Msa1174460:three_prime_UTR 20.0%
! TAGTTATAGATCAGTTTCTA+GGG - chr8_1:5553989-5554008 None:intergenic 25.0%
! TTAGTTATAGATCAGTTTCT+AGG - chr8_1:5553990-5554009 None:intergenic 25.0%
!!! GTTTTGAATTTACCTTCAAT+TGG + chr8_1:5554023-5554042 Msa1174460:intron 25.0%
! AATTCCTCCATATAATATTG+CGG + chr8_1:5554323-5554342 Msa1174460:intron 25.0%
! TAGATAAGTGTTTATAAGTG+GGG - chr8_1:5554573-5554592 None:intergenic 25.0%
! CATAGATAAGTGTTTATAAG+TGG - chr8_1:5554575-5554594 None:intergenic 25.0%
!! TGAATAAACTCTTTTACTGT+TGG + chr8_1:5555281-5555300 Msa1174460:intron 25.0%
!! GAATAAACTCTTTTACTGTT+GGG + chr8_1:5555282-5555301 Msa1174460:intron 25.0%
! TCTTAAAGTCTTAAGAACAA+AGG + chr8_1:5555335-5555354 Msa1174460:intron 25.0%
! ATTATCGTCGTTATCAAATA+TGG - chr8_1:5555772-5555791 None:intergenic 25.0%
! TCACACTAGAATGTAAATAA+CGG + chr8_1:5556165-5556184 Msa1174460:exon 25.0%
! TTATGACAAACATAAACCAT+GGG - chr8_1:5556263-5556282 None:intergenic 25.0%
! TTTATGACAAACATAAACCA+TGG - chr8_1:5556264-5556283 None:intergenic 25.0%
! ATGGTTTATGTTTGTCATAA+AGG + chr8_1:5556263-5556282 Msa1174460:three_prime_UTR 25.0%
!!! CATTTGTTGTAAAATAAGGA+TGG + chr8_1:5556290-5556309 Msa1174460:three_prime_UTR 25.0%
AATTGGAAAGAGACAAGTAA+GGG - chr8_1:5553655-5553674 None:intergenic 30.0%
AGTTAGAATGAAGAACATGT+TGG + chr8_1:5554049-5554068 Msa1174460:intron 30.0%
AGATAAGTGTTTATAAGTGG+GGG - chr8_1:5554572-5554591 None:intergenic 30.0%
!!! AACATGTTTTGTTTGTGTTG+TGG + chr8_1:5554698-5554717 Msa1174460:intron 30.0%
!!! ACATGTTTTGTTTGTGTTGT+GGG + chr8_1:5554699-5554718 Msa1174460:intron 30.0%
AATTGTATGGAAATTCCATG+TGG + chr8_1:5554745-5554764 Msa1174460:intron 30.0%
ATTGTATGGAAATTCCATGT+GGG + chr8_1:5554746-5554765 Msa1174460:intron 30.0%
!! ATTTATTTGGGTTTTGACAG+CGG + chr8_1:5555065-5555084 Msa1174460:intron 30.0%
GTAAAAGAGTTTATTCAGAC+AGG - chr8_1:5555278-5555297 None:intergenic 30.0%
GGAATTTCATATGTATCAGA+AGG + chr8_1:5555356-5555375 Msa1174460:intron 30.0%
! GTGAATTGTAAAGCATTCTT+TGG + chr8_1:5555506-5555525 Msa1174460:intron 30.0%
AATATGAAGTGTAGAGTTCA+GGG - chr8_1:5555536-5555555 None:intergenic 30.0%
TAATATGAAGTGTAGAGTTC+AGG - chr8_1:5555537-5555556 None:intergenic 30.0%
TATGTTGCAAATTGTTCAAC+TGG + chr8_1:5555633-5555652 Msa1174460:intron 30.0%
GCAAACTCTTTATGAGTTTA+CGG + chr8_1:5555868-5555887 Msa1174460:intron 30.0%
AAACATTAACACATGAGAGA+AGG - chr8_1:5555992-5556011 None:intergenic 30.0%
!! GATCATTTTTCCTGATGTAT+AGG + chr8_1:5556012-5556031 Msa1174460:intron 30.0%
TTGTATTCAGCAAAGTTTAC+AGG - chr8_1:5556202-5556221 None:intergenic 30.0%
! GGTAAATGTGAACTTTTGTT+TGG + chr8_1:5556311-5556330 Msa1174460:three_prime_UTR 30.0%
!!! ATGTTGCTCTTTTTTATTCC+CGG + chr8_1:5556347-5556366 Msa1174460:three_prime_UTR 30.0%
! TCACTGGAAGAAGATTCTTT+TGG - chr8_1:5553479-5553498 None:intergenic 35.0%
GAATTGGAAAGAGACAAGTA+AGG - chr8_1:5553656-5553675 None:intergenic 35.0%
!!! TAGGTCAAATTGACTGGTTT+TGG - chr8_1:5553711-5553730 None:intergenic 35.0%
ATGTCGCAGAAATTAACTCT+AGG + chr8_1:5553896-5553915 Msa1174460:CDS 35.0%
ATTCTAACTCAGCCAATTGA+AGG - chr8_1:5554038-5554057 None:intergenic 35.0%
GAGAAGACTGATACAACTAT+TGG - chr8_1:5554287-5554306 None:intergenic 35.0%
TGTATCAGTCTTCTCAATAG+TGG + chr8_1:5554292-5554311 Msa1174460:intron 35.0%
! GGCCATAAATTTTGAACAGT+GGG + chr8_1:5554377-5554396 Msa1174460:intron 35.0%
! GCCATAAATTTTGAACAGTG+GGG + chr8_1:5554378-5554397 Msa1174460:intron 35.0%
! CCATAAATTTTGAACAGTGG+GGG + chr8_1:5554379-5554398 Msa1174460:intron 35.0%
! ACCATTGCACGAAATTTGTT+TGG + chr8_1:5554403-5554422 Msa1174460:intron 35.0%
TCCAAACAAATTTCGTGCAA+TGG - chr8_1:5554407-5554426 None:intergenic 35.0%
! ATTGCACGAAATTTGTTTGG+AGG + chr8_1:5554406-5554425 Msa1174460:intron 35.0%
! TTGCACGAAATTTGTTTGGA+GGG + chr8_1:5554407-5554426 Msa1174460:intron 35.0%
!! TTTTTCGATTCCATGAAACG+AGG + chr8_1:5554447-5554466 Msa1174460:intron 35.0%
AGACTCTTTAACAGATAGTG+AGG + chr8_1:5554614-5554633 Msa1174460:intron 35.0%
!!! CATGTTTTGTTTGTGTTGTG+GGG + chr8_1:5554700-5554719 Msa1174460:intron 35.0%
!!! ATGTTTTGTTTGTGTTGTGG+GGG + chr8_1:5554701-5554720 Msa1174460:intron 35.0%
TGGGGGAAATCAAAATAAAC+TGG + chr8_1:5554718-5554737 Msa1174460:intron 35.0%
GATGTCAAATATCTTTGTGG+AGG + chr8_1:5554789-5554808 Msa1174460:intron 35.0%
!! TTTATTTGGGTTTTGACAGC+GGG + chr8_1:5555066-5555085 Msa1174460:intron 35.0%
ACATAGCCATGTCTAAATTC+TGG + chr8_1:5555470-5555489 Msa1174460:intron 35.0%
ATATGAAGTGTAGAGTTCAG+GGG - chr8_1:5555535-5555554 None:intergenic 35.0%
TAGAACCTACCATTTACCAA+AGG + chr8_1:5555598-5555617 Msa1174460:intron 35.0%
CATAATTCGTTTGATGCCTT+TGG - chr8_1:5555617-5555636 None:intergenic 35.0%
GCAAATTGTTCAACTGGTTA+TGG + chr8_1:5555639-5555658 Msa1174460:intron 35.0%
ACAATGATGCATCATTCCAT+TGG + chr8_1:5555667-5555686 Msa1174460:intron 35.0%
GTTTCTTTGCCTTGAAGTTT+AGG + chr8_1:5555735-5555754 Msa1174460:intron 35.0%
TAGAACAGTCCTAAACTTCA+AGG - chr8_1:5555747-5555766 None:intergenic 35.0%
! CTCGCTTCCTGAATTTATTT+TGG + chr8_1:5555826-5555845 Msa1174460:intron 35.0%
! ATCAATTCTAGTTGCTCACA+AGG - chr8_1:5555938-5555957 None:intergenic 35.0%
GTTCTCGTTTCAAGAAATCA+GGG + chr8_1:5556064-5556083 Msa1174460:CDS 35.0%
! TCCCCTCTTGTTATGAAATT+TGG + chr8_1:5556222-5556241 Msa1174460:three_prime_UTR 35.0%
CCCAAATTTCATAACAAGAG+GGG - chr8_1:5556226-5556245 None:intergenic 35.0%
! CCCCTCTTGTTATGAAATTT+GGG + chr8_1:5556223-5556242 Msa1174460:three_prime_UTR 35.0%
CCCCAAATTTCATAACAAGA+GGG - chr8_1:5556227-5556246 None:intergenic 35.0%
! CCCTCTTGTTATGAAATTTG+GGG + chr8_1:5556224-5556243 Msa1174460:three_prime_UTR 35.0%
ACCCCAAATTTCATAACAAG+AGG - chr8_1:5556228-5556247 None:intergenic 35.0%
! TGAACCTATGCATATGCTTT+TGG + chr8_1:5556377-5556396 Msa1174460:three_prime_UTR 35.0%
! AGTTCCAAAAGCATATGCAT+AGG - chr8_1:5556384-5556403 None:intergenic 35.0%
!!! ATGCATATGCTTTTGGAACT+TGG + chr8_1:5556384-5556403 Msa1174460:three_prime_UTR 35.0%
! CCACTCTTTCGTCTTTTGTA+AGG - chr8_1:5553435-5553454 None:intergenic 40.0%
CCTTACAAAAGACGAAAGAG+TGG + chr8_1:5553432-5553451 Msa1174460:five_prime_UTR 40.0%
TTTCTGCCATTGACGAAAGA+AGG - chr8_1:5553514-5553533 None:intergenic 40.0%
! AGCGAGTTGAGAATGTGAAT+TGG - chr8_1:5553672-5553691 None:intergenic 40.0%
AAAGGAAACGGAGACGATTT+CGG + chr8_1:5553773-5553792 Msa1174460:CDS 40.0%
CTAGGTTATGCTATTCTCGT+TGG + chr8_1:5553914-5553933 Msa1174460:CDS 40.0%
AGTGCCGCAATATTATATGG+AGG - chr8_1:5554330-5554349 None:intergenic 40.0%
! GGGCCATAAATTTTGAACAG+TGG + chr8_1:5554376-5554395 Msa1174460:intron 40.0%
CCCCCACTGTTCAAAATTTA+TGG - chr8_1:5554382-5554401 None:intergenic 40.0%
! AATTTGTTTGGAGGGAGAGT+TGG + chr8_1:5554415-5554434 Msa1174460:intron 40.0%
AGAGAGAAAGAGATGATCGT+AGG + chr8_1:5554497-5554516 Msa1174460:intron 40.0%
AGAAAGAGATGATCGTAGGT+GGG + chr8_1:5554501-5554520 Msa1174460:intron 40.0%
TCTGTTAAAGAGTCTCACCT+CGG - chr8_1:5554609-5554628 None:intergenic 40.0%
TCTTTAACAGATAGTGAGGC+AGG + chr8_1:5554618-5554637 Msa1174460:intron 40.0%
CTTTAACAGATAGTGAGGCA+GGG + chr8_1:5554619-5554638 Msa1174460:intron 40.0%
GGGGATGTCAAATATCTTTG+TGG + chr8_1:5554786-5554805 Msa1174460:intron 40.0%
TCAAATATCTTTGTGGAGGC+AGG + chr8_1:5554793-5554812 Msa1174460:intron 40.0%
!! TATGTTTTTACCGAGCTACC+CGG + chr8_1:5555165-5555184 Msa1174460:CDS 40.0%
TAGCCATGTCTAAATTCTGG+AGG + chr8_1:5555473-5555492 Msa1174460:intron 40.0%
CAACCTCCAGAATTTAGACA+TGG - chr8_1:5555479-5555498 None:intergenic 40.0%
TGATGCCTTTGGTAAATGGT+AGG - chr8_1:5555606-5555625 None:intergenic 40.0%
CGTTTGATGCCTTTGGTAAA+TGG - chr8_1:5555610-5555629 None:intergenic 40.0%
AGAGCACAATTGTGTACCAA+TGG - chr8_1:5555686-5555705 None:intergenic 40.0%
TGGTACACAATTGTGCTCTT+TGG + chr8_1:5555687-5555706 Msa1174460:intron 40.0%
GAGCTAGAAAAATTCCACAC+TGG - chr8_1:5555809-5555828 None:intergenic 40.0%
AGCAGATATGCCTATACATC+AGG - chr8_1:5556025-5556044 None:intergenic 40.0%
GGTTCTCGTTTCAAGAAATC+AGG + chr8_1:5556063-5556082 Msa1174460:CDS 40.0%
TCGTTTCAAGAAATCAGGGA+AGG + chr8_1:5556068-5556087 Msa1174460:CDS 40.0%
! CTTGTGTCGTTCATAATGAC+CGG + chr8_1:5556114-5556133 Msa1174460:CDS 40.0%
AATTCCATGCAAGTAACCAC+CGG - chr8_1:5556136-5556155 None:intergenic 40.0%
TGCATAGGTTCAAACACAAC+CGG - chr8_1:5556369-5556388 None:intergenic 40.0%
!!! AAGGTTCTGGTTTTGCTCAC+TGG - chr8_1:5553495-5553514 None:intergenic 45.0%
CCATTGACGAAAGAAGGTTC+TGG - chr8_1:5553508-5553527 None:intergenic 45.0%
CCAGAACCTTCTTTCGTCAA+TGG + chr8_1:5553505-5553524 Msa1174460:exon 45.0%
! AGAAATAGTGGTGGGAGTGT+GGG - chr8_1:5553603-5553622 None:intergenic 45.0%
AAGGAGCGAGAAATAGTGGT+GGG - chr8_1:5553611-5553630 None:intergenic 45.0%
AAGGAAGGAGCGAGAAATAG+TGG - chr8_1:5553615-5553634 None:intergenic 45.0%
GACAAGTAAGGGAAAGAAGC+AGG - chr8_1:5553644-5553663 None:intergenic 45.0%
GGCACTTAGGTCAAATTGAC+TGG - chr8_1:5553717-5553736 None:intergenic 45.0%
GGCGGTGATATCAAAGGAAA+CGG + chr8_1:5553761-5553780 Msa1174460:CDS 45.0%
GGAGGAGACGATAGTAACAA+AGG + chr8_1:5553830-5553849 Msa1174460:CDS 45.0%
AGACGATAGTAACAAAGGCG+AGG + chr8_1:5553835-5553854 Msa1174460:CDS 45.0%
GATAGTAACAAAGGCGAGGA+AGG + chr8_1:5553839-5553858 Msa1174460:CDS 45.0%
AAGGATCTGACGGTGATAAG+AGG + chr8_1:5553858-5553877 Msa1174460:CDS 45.0%
TGACGGTGATAAGAGGAAGA+TGG + chr8_1:5553865-5553884 Msa1174460:CDS 45.0%
GGTTATGCTATTCTCGTTGG+AGG + chr8_1:5553917-5553936 Msa1174460:CDS 45.0%
GGCAGTGCCGCAATATTATA+TGG - chr8_1:5554333-5554352 None:intergenic 45.0%
CACTGCCATCCTTCACTAAT+TGG + chr8_1:5554346-5554365 Msa1174460:intron 45.0%
TGCCATCCTTCACTAATTGG+CGG + chr8_1:5554349-5554368 Msa1174460:intron 45.0%
TCGATTCCATGAAACGAGGA+AGG + chr8_1:5554451-5554470 Msa1174460:intron 45.0%
CGATTCCATGAAACGAGGAA+GGG + chr8_1:5554452-5554471 Msa1174460:intron 45.0%
GAAAACCCTTCCTCGTTTCA+TGG - chr8_1:5554460-5554479 None:intergenic 45.0%
GAGAAAGAGATGATCGTAGG+TGG + chr8_1:5554500-5554519 Msa1174460:intron 45.0%
TCTCACAAAAACGGCATGTG+AGG + chr8_1:5554537-5554556 Msa1174460:intron 45.0%
TCTATGTCATCTCTCAACCG+AGG + chr8_1:5554589-5554608 Msa1174460:intron 45.0%
TCCATGTGGGTAGACAAGTA+TGG + chr8_1:5554759-5554778 Msa1174460:intron 45.0%
ACCATACTTGTCTACCCACA+TGG - chr8_1:5554763-5554782 None:intergenic 45.0%
TGTGGGTAGACAAGTATGGT+AGG + chr8_1:5554763-5554782 Msa1174460:intron 45.0%
GTGGGTAGACAAGTATGGTA+GGG + chr8_1:5554764-5554783 Msa1174460:intron 45.0%
TGGGTAGACAAGTATGGTAG+GGG + chr8_1:5554765-5554784 Msa1174460:intron 45.0%
TCAAATATCTGCCACCACAG+TGG - chr8_1:5554847-5554866 None:intergenic 45.0%
!! GTAATGGGCTTTCTGAAGAG+TGG + chr8_1:5555093-5555112 Msa1174460:CDS 45.0%
TGATGCAAGAACAGGTCTTC+CGG - chr8_1:5555187-5555206 None:intergenic 45.0%
ACCTGTTCTTGCATCATCTG+TGG + chr8_1:5555191-5555210 Msa1174460:CDS 45.0%
CCTGTTCTTGCATCATCTGT+GGG + chr8_1:5555192-5555211 Msa1174460:CDS 45.0%
TGGCTATGTTTCAGCCTTAG+CGG - chr8_1:5555459-5555478 None:intergenic 45.0%
! TTTAGCACTGAAGTCCAGTG+TGG + chr8_1:5555792-5555811 Msa1174460:intron 45.0%
CATATCTGCTGCACTTCTTG+TGG + chr8_1:5556035-5556054 Msa1174460:CDS 45.0%
GAAATCAGGGAAGGTCTTTC+CGG + chr8_1:5556077-5556096 Msa1174460:CDS 45.0%
GCATAGGTTCAAACACAACC+GGG - chr8_1:5556368-5556387 None:intergenic 45.0%
TCCTCTCAATCTGTTTCGCT+GGG - chr8_1:5556442-5556461 None:intergenic 45.0%
GTGGCCCAATACAGAAACCA+CGG + chr8_1:5553451-5553470 Msa1174460:five_prime_UTR 50.0%
! AGAAGATTCTTTTGGCGCCG+TGG - chr8_1:5553471-5553490 None:intergenic 50.0%
GTCAATGGCAGAAACTGCAG+CGG + chr8_1:5553520-5553539 Msa1174460:exon 50.0%
! GCAGAAACTGCAGCGGTTTT+CGG + chr8_1:5553527-5553546 Msa1174460:CDS 50.0%
! GAGAAATAGTGGTGGGAGTG+TGG - chr8_1:5553604-5553623 None:intergenic 50.0%
GAAGGAGCGAGAAATAGTGG+TGG - chr8_1:5553612-5553631 None:intergenic 50.0%
AGAAGCAGGAGAAGCAAGGA+AGG - chr8_1:5553630-5553649 None:intergenic 50.0%
GGAAAGAAGCAGGAGAAGCA+AGG - chr8_1:5553634-5553653 None:intergenic 50.0%
!! TGTCTAAATCGGGGGCACTT+AGG - chr8_1:5553730-5553749 None:intergenic 50.0%
GCCCCCGATTTAGACATTAC+CGG + chr8_1:5553734-5553753 Msa1174460:CDS 50.0%
GCCGGTAATGTCTAAATCGG+GGG - chr8_1:5553738-5553757 None:intergenic 50.0%
CGCCGGTAATGTCTAAATCG+GGG - chr8_1:5553739-5553758 None:intergenic 50.0%
CCGCCGGTAATGTCTAAATC+GGG - chr8_1:5553740-5553759 None:intergenic 50.0%
CCCGATTTAGACATTACCGG+CGG + chr8_1:5553737-5553756 Msa1174460:CDS 50.0%
GCCGCCGGTAATGTCTAAAT+CGG - chr8_1:5553741-5553760 None:intergenic 50.0%
GATTTAGACATTACCGGCGG+CGG + chr8_1:5553740-5553759 Msa1174460:CDS 50.0%
AAAGGCGAGGAAGGATCTGA+CGG + chr8_1:5553848-5553867 Msa1174460:CDS 50.0%
CACCGCCAATTAGTGAAGGA+TGG - chr8_1:5554354-5554373 None:intergenic 50.0%
CTTCACTAATTGGCGGTGCA+GGG + chr8_1:5554356-5554375 Msa1174460:intron 50.0%
GGGTAGACAAGTATGGTAGG+GGG + chr8_1:5554766-5554785 Msa1174460:intron 50.0%
GGTAGACAAGTATGGTAGGG+GGG + chr8_1:5554767-5554786 Msa1174460:intron 50.0%
AGCCAGACACAAGGTTGTGT+GGG - chr8_1:5554821-5554840 None:intergenic 50.0%
AATATCTGCCACCACAGTGG+TGG - chr8_1:5554844-5554863 None:intergenic 50.0%
!! ATTTGGGTTTTGACAGCGGG+TGG + chr8_1:5555069-5555088 Msa1174460:intron 50.0%
TTTGACAGCGGGTGGTGTAA+TGG + chr8_1:5555077-5555096 Msa1174460:intron 50.0%
TTGACAGCGGGTGGTGTAAT+GGG + chr8_1:5555078-5555097 Msa1174460:intron 50.0%
!! TGGCAGCCAGAAGTCACTTT+TGG + chr8_1:5555113-5555132 Msa1174460:CDS 50.0%
!!! AGCCAGAAGTCACTTTTGGC+TGG + chr8_1:5555117-5555136 Msa1174460:CDS 50.0%
!!! GCCAGAAGTCACTTTTGGCT+GGG + chr8_1:5555118-5555137 Msa1174460:CDS 50.0%
!!! CCAGAAGTCACTTTTGGCTG+GGG + chr8_1:5555119-5555138 Msa1174460:CDS 50.0%
!!! CAGAAGTCACTTTTGGCTGG+GGG + chr8_1:5555120-5555139 Msa1174460:CDS 50.0%
GATGCAAGAACAGGTCTTCC+GGG - chr8_1:5555186-5555205 None:intergenic 50.0%
CCCACAGATGATGCAAGAAC+AGG - chr8_1:5555195-5555214 None:intergenic 50.0%
AGCTCAGCAGTGACTTACCA+AGG - chr8_1:5555218-5555237 None:intergenic 50.0%
TCTTGAGTTTCTGCCCGCTA+AGG + chr8_1:5555442-5555461 Msa1174460:intron 50.0%
GGCTATGTTTCAGCCTTAGC+GGG - chr8_1:5555458-5555477 None:intergenic 50.0%
TGCTGCACTTCTTGTGGTGA+TGG + chr8_1:5556041-5556060 Msa1174460:CDS 50.0%
GCTGCACTTCTTGTGGTGAT+GGG + chr8_1:5556042-5556061 Msa1174460:CDS 50.0%
ACAAGTGACACAACACCTGC+CGG - chr8_1:5556099-5556118 None:intergenic 50.0%
! GTGTCGTTCATAATGACCGG+TGG + chr8_1:5556117-5556136 Msa1174460:CDS 50.0%
ATGACCGGTGGTTACTTGCA+TGG + chr8_1:5556129-5556148 Msa1174460:CDS 50.0%
ACCCAGCGAAACAGATTGAG+AGG + chr8_1:5556438-5556457 Msa1174460:three_prime_UTR 50.0%
CTCCTCTCAATCTGTTTCGC+TGG - chr8_1:5556443-5556462 None:intergenic 50.0%
GGCGCCGTGGTTTCTGTATT+GGG - chr8_1:5553458-5553477 None:intergenic 55.0%
TGGCGCCGTGGTTTCTGTAT+TGG - chr8_1:5553459-5553478 None:intergenic 55.0%
GGTTGTAGGGCGAAGAGAGA+GGG - chr8_1:5553576-5553595 None:intergenic 55.0%
! GAGTGTGGGATCGGGTTGTA+GGG - chr8_1:5553589-5553608 None:intergenic 55.0%
! TAGTGGTGGGAGTGTGGGAT+CGG - chr8_1:5553598-5553617 None:intergenic 55.0%
GGAAACGGAGACGATTTCGG+CGG + chr8_1:5553776-5553795 Msa1174460:CDS 55.0%
CCTGCACCGCCAATTAGTGA+AGG - chr8_1:5554358-5554377 None:intergenic 55.0%
CCTTCACTAATTGGCGGTGC+AGG + chr8_1:5554355-5554374 Msa1174460:intron 55.0%
!! GTTTGGAGGGAGAGTTGGTG+TGG + chr8_1:5554420-5554439 Msa1174460:intron 55.0%
AGCCCACACAACCTTGTGTC+TGG + chr8_1:5554816-5554835 Msa1174460:intron 55.0%
GAGCCAGACACAAGGTTGTG+TGG - chr8_1:5554822-5554841 None:intergenic 55.0%
CCCCAGCCAAAAGTGACTTC+TGG - chr8_1:5555122-5555141 None:intergenic 55.0%
AACAGGTCTTCCGGGTAGCT+CGG - chr8_1:5555178-5555197 None:intergenic 55.0%
CTTGCATCATCTGTGGGCCT+TGG + chr8_1:5555198-5555217 Msa1174460:CDS 55.0%
GGGTTTGTGCTGTAGACCCA+TGG + chr8_1:5556244-5556263 Msa1174460:three_prime_UTR 55.0%
GTAGGGCGAAGAGAGAGGGA+AGG - chr8_1:5553572-5553591 None:intergenic 60.0%
GGGTTGTAGGGCGAAGAGAG+AGG - chr8_1:5553577-5553596 None:intergenic 60.0%
! GGAGTGTGGGATCGGGTTGT+AGG - chr8_1:5553590-5553609 None:intergenic 60.0%
! AGTGGTGGGAGTGTGGGATC+GGG - chr8_1:5553597-5553616 None:intergenic 60.0%
TTAGACATTACCGGCGGCGG+CGG + chr8_1:5553743-5553762 Msa1174460:CDS 60.0%
TTTGATATCACCGCCGCCGC+CGG - chr8_1:5553756-5553775 None:intergenic 60.0%
AACGGAGACGATTTCGGCGG+AGG + chr8_1:5553779-5553798 Msa1174460:CDS 60.0%
! CAGTGGTGGAGCCAGACACA+AGG - chr8_1:5554830-5554849 None:intergenic 60.0%
! TGTGTCTGGCTCCACCACTG+TGG + chr8_1:5554830-5554849 Msa1174460:intron 60.0%
TCAGGGAAGGTCTTTCCGGC+AGG + chr8_1:5556081-5556100 Msa1174460:CDS 60.0%
GGCGGCGGCGGTGATATCAA+AGG + chr8_1:5553755-5553774 Msa1174460:CDS 65.0%
GGAGACGATTTCGGCGGAGG+AGG + chr8_1:5553782-5553801 Msa1174460:CDS 65.0%
GACGATTTCGGCGGAGGAGG+TGG + chr8_1:5553785-5553804 Msa1174460:CDS 65.0%
GATTTCGGCGGAGGAGGTGG+TGG + chr8_1:5553788-5553807 Msa1174460:CDS 65.0%
!! GGAGGTGGTGGTGGTGGTGA+AGG + chr8_1:5553800-5553819 Msa1174460:CDS 65.0%
!! GGTGGTGGTGGTGGTGAAGG+AGG + chr8_1:5553803-5553822 Msa1174460:CDS 65.0%
!! GGTGGTGGTGGTGAAGGAGG+AGG + chr8_1:5553806-5553825 Msa1174460:CDS 65.0%
! GGTGGTGGTGAAGGAGGAGG+CGG + chr8_1:5553809-5553828 Msa1174460:CDS 65.0%
! GTCTGGCTCCACCACTGTGG+TGG + chr8_1:5554833-5554852 Msa1174460:intron 65.0%
! TTCGGCGGAGGAGGTGGTGG+TGG + chr8_1:5553791-5553810 Msa1174460:CDS 70.0%
GGTGGTGAAGGAGGAGGCGG+AGG + chr8_1:5553812-5553831 Msa1174460:CDS 70.0%
!! GGCGGAGGAGGTGGTGGTGG+TGG + chr8_1:5553794-5553813 Msa1174460:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 5553419 5556472 5553419 ID=Msa1174460;Name=Msa1174460
chr8_1 mRNA 5553419 5556472 5553419 ID=Msa1174460-mRNA-1;Parent=Msa1174460;Name=Msa1174460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=105|1|1|1|1|1|3|299|229
chr8_1 exon 5553419 5553938 5553419 ID=Msa1174460-mRNA-1:exon:1829;Parent=Msa1174460-mRNA-1
chr8_1 exon 5555085 5555219 5555085 ID=Msa1174460-mRNA-1:exon:1830;Parent=Msa1174460-mRNA-1
chr8_1 exon 5556034 5556472 5556034 ID=Msa1174460-mRNA-1:exon:1831;Parent=Msa1174460-mRNA-1
chr8_1 five_prime_UTR 5553419 5553523 5553419 ID=Msa1174460-mRNA-1:five_prime_utr;Parent=Msa1174460-mRNA-1
chr8_1 CDS 5553524 5553938 5553524 ID=Msa1174460-mRNA-1:cds;Parent=Msa1174460-mRNA-1
chr8_1 CDS 5555085 5555219 5555085 ID=Msa1174460-mRNA-1:cds;Parent=Msa1174460-mRNA-1
chr8_1 CDS 5556034 5556173 5556034 ID=Msa1174460-mRNA-1:cds;Parent=Msa1174460-mRNA-1
chr8_1 three_prime_UTR 5556174 5556472 5556174 ID=Msa1174460-mRNA-1:three_prime_utr;Parent=Msa1174460-mRNA-1
Gene Sequence

>Msa1174460

ATGGCAGAAACTGCAGCGGTTTTCGGTGTTTGCAGTTCAACACTTCCTTCCCTCTCTCTTCGCCCTACAACCCGATCCCACACTCCCACCACTATTTCTCGCTCCTTCCTTGCTTCTCCTGCTTCTTTCCCTTACTTGTCTCTTTCCAATTCACATTCTCAACTCGCTGTTGTTTCTTCTGATTCCAAAACCAGTCAATTTGACCTAAGTGCCCCCGATTTAGACATTACCGGCGGCGGCGGTGATATCAAAGGAAACGGAGACGATTTCGGCGGAGGAGGTGGTGGTGGTGGTGAAGGAGGAGGCGGAGGAGACGATAGTAACAAAGGCGAGGAAGGATCTGACGGTGATAAGAGGAAGATGGCTTTGTCAATGTCGCAGAAATTAACTCTAGGTTATGCTATTCTCGTTGGAGCGGGTGGTGTAATGGGCTTTCTGAAGAGTGGCAGCCAGAAGTCACTTTTGGCTGGGGGTTTGTCTTCTGCACTGCTGTTTTATGTTTTTACCGAGCTACCCGGAAGACCTGTTCTTGCATCATCTGTGGGCCTTGGCATATCTGCTGCACTTCTTGTGGTGATGGGTTCTCGTTTCAAGAAATCAGGGAAGGTCTTTCCGGCAGGTGTTGTGTCACTTGTGTCGTTCATAATGACCGGTGGTTACTTGCATGGAATTATGCGTAGTTCACACTAG
Protein sequence

>Msa1174460

MAETAAVFGVCSSTLPSLSLRPTTRSHTPTTISRSFLASPASFPYLSLSNSHSQLAVVSSDSKTSQFDLSAPDLDITGGGGDIKGNGDDFGGGGGGGGEGGGGGDDSNKGEEGSDGDKRKMALSMSQKLTLGYAILVGAGGVMGFLKSGSQKSLLAGGLSSALLFYVFTELPGRPVLASSVGLGISAALLVVMGSRFKKSGKVFPAGVVSLVSFIMTGGYLHGIMRSSH*