AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0139340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0139340 MtrunA17_Chr4g0069691 93.450 229 14 1 1 229 1 228 1.18e-119 339
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0139340 AT3G43520.1 69.748 119 35 1 111 229 123 240 3.96e-51 166
Msa0139340 AT2G26240.1 63.810 105 38 0 124 228 4 108 3.74e-38 129

Find 107 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCCTCTTGTTATGAAATT+TGG 0.026882 1_3:+72999063 Msa0139340:three_prime_UTR
CCCCTCTTGTTATGAAATTT+GGG 0.209009 1_3:+72999064 Msa0139340:three_prime_UTR
TAGACATTTGTTGTAAAATA+AGG 0.232439 1_3:+72999127 Msa0139340:three_prime_UTR
GGCGCCGTGGTTTCTGTATT+GGG 0.247815 1_3:-72996297 None:intergenic
TAGGTCAAATTGACTGGTTT+TGG 0.265563 1_3:-72996550 None:intergenic
TGGCGCCGTGGTTTCTGTAT+TGG 0.272823 1_3:-72996298 None:intergenic
TGAACCTATGCATATGCTTT+TGG 0.274183 1_3:+72999218 Msa0139340:three_prime_UTR
GAAATCAGGGAAGGTCTTTC+CGG 0.282767 1_3:+72998918 Msa0139340:CDS
CTCCTCTCAATCTGTTTCGC+TGG 0.292658 1_3:-72999281 None:intergenic
GGAGTGTGGGATCGGGTTGT+AGG 0.296999 1_3:-72996429 None:intergenic
TTGTATTCAGCAAAGTTTAC+AGG 0.324932 1_3:-72999040 None:intergenic
GGTTCTCGTTTCAAGAAATC+AGG 0.336113 1_3:+72998904 Msa0139340:CDS
AAAGGAAACGGAGACGATTT+CGG 0.340053 1_3:+72996615 Msa0139340:CDS
TTGACAGCGGGTGGTGTAAT+GGG 0.345679 1_3:+72997919 Msa0139340:intron
TGATGCAAGAACAGGTCTTC+CGG 0.347788 1_3:-72998025 None:intergenic
TTTGACAGCGGGTGGTGTAA+TGG 0.350276 1_3:+72997918 Msa0139340:intron
TGGCAGCCAGAAGTCACTTT+TGG 0.366373 1_3:+72997954 Msa0139340:CDS
GGTGGTGGTGGTGAAGGAGG+AGG 0.373824 1_3:+72996648 Msa0139340:CDS
GGTGGTGAAGGAGGAGGCGG+AGG 0.375033 1_3:+72996654 Msa0139340:CDS
ATGGTTTATGTTTGTCATAA+AGG 0.380251 1_3:+72999104 Msa0139340:three_prime_UTR
TGCTGCACTTCTTGTGGTGA+TGG 0.380439 1_3:+72998882 Msa0139340:CDS
GCCGCCGGTAATGTCTAAAT+CGG 0.388850 1_3:-72996580 None:intergenic
GGTGGTGGTGAAGGAGGAGG+CGG 0.392210 1_3:+72996651 Msa0139340:CDS
TGCATAGGTTCAAACACAAC+CGG 0.412204 1_3:-72999207 None:intergenic
CCGCCGGTAATGTCTAAATC+GGG 0.413389 1_3:-72996579 None:intergenic
CCCCAGCCAAAAGTGACTTC+TGG 0.415128 1_3:-72997960 None:intergenic
TCACTGGAAGAAGATTCTTT+TGG 0.418232 1_3:-72996318 None:intergenic
TAGTGGTGGGAGTGTGGGAT+CGG 0.421797 1_3:-72996437 None:intergenic
CCATTGACGAAAGAAGGTTC+TGG 0.425009 1_3:-72996347 None:intergenic
GACGATTTCGGCGGAGGAGG+TGG 0.426057 1_3:+72996627 Msa0139340:CDS
CTTGCATCATCTGTGGGCCT+TGG 0.426584 1_3:+72998039 Msa0139340:CDS
CCAGAACCTTCTTTCGTCAA+TGG 0.430434 1_3:+72996347 Msa0139340:exon
GATGCAAGAACAGGTCTTCC+GGG 0.430490 1_3:-72998024 None:intergenic
GAGAAATAGTGGTGGGAGTG+TGG 0.440353 1_3:-72996443 None:intergenic
GCTGCACTTCTTGTGGTGAT+GGG 0.443502 1_3:+72998883 Msa0139340:CDS
AGCGAGTTGAGAATGTGAAT+TGG 0.445867 1_3:-72996511 None:intergenic
GGCGGAGGAGGTGGTGGTGG+TGG 0.449465 1_3:+72996636 Msa0139340:CDS
TCCTCTCAATCTGTTTCGCT+GGG 0.449558 1_3:-72999280 None:intergenic
AGTGGTGGGAGTGTGGGATC+GGG 0.454693 1_3:-72996436 None:intergenic
TTTCTGCCATTGACGAAAGA+AGG 0.457559 1_3:-72996353 None:intergenic
TTCGGCGGAGGAGGTGGTGG+TGG 0.461763 1_3:+72996633 Msa0139340:CDS
GATTTCGGCGGAGGAGGTGG+TGG 0.468875 1_3:+72996630 Msa0139340:CDS
GACAAGTAAGGGGAAGAAGC+AGG 0.473313 1_3:-72996483 None:intergenic
GGCACTTAGGTCAAATTGAC+TGG 0.474158 1_3:-72996556 None:intergenic
ACCTGTTCTTGCATCATCTG+TGG 0.483230 1_3:+72998032 Msa0139340:CDS
AACGGAGACGATTTCGGCGG+AGG 0.486679 1_3:+72996621 Msa0139340:CDS
CCCACAGATGATGCAAGAAC+AGG 0.490656 1_3:-72998033 None:intergenic
CTAGGTTATGCTATTCTCGT+TGG 0.491350 1_3:+72996756 Msa0139340:CDS
AGAAGCAGGAGAAGCAAGGA+AGG 0.491822 1_3:-72996469 None:intergenic
AAGGAAGGAGCGAGAAATAG+TGG 0.498256 1_3:-72996454 None:intergenic
AAGGATCTGACGGTGATAAG+AGG 0.499849 1_3:+72996700 Msa0139340:CDS
AACAGGTCTTCCGGGTAGCT+CGG 0.505808 1_3:-72998016 None:intergenic
TTTGATATCACCGCCGCCGC+CGG 0.506217 1_3:-72996595 None:intergenic
TTAGACATTACCGGCGGCGG+CGG 0.514783 1_3:+72996585 Msa0139340:CDS
GGTTATGCTATTCTCGTTGG+AGG 0.515433 1_3:+72996759 Msa0139340:CDS
GATTGAGAGGAGTAAACATA+AGG 0.520080 1_3:+72999292 Msa0139340:three_prime_UTR
CCTGTTCTTGCATCATCTGT+GGG 0.521720 1_3:+72998033 Msa0139340:CDS
GGAGACGATTTCGGCGGAGG+AGG 0.526381 1_3:+72996624 Msa0139340:CDS
GGGTTTGTGCTGTAGACCCA+TGG 0.529398 1_3:+72999085 Msa0139340:three_prime_UTR
GAGTGTGGGATCGGGTTGTA+GGG 0.536706 1_3:-72996428 None:intergenic
GGCGGTGATATCAAAGGAAA+CGG 0.540755 1_3:+72996603 Msa0139340:CDS
GATAGTAACAAAGGCGAGGA+AGG 0.548487 1_3:+72996681 Msa0139340:CDS
CATATCTGCTGCACTTCTTG+TGG 0.550309 1_3:+72998876 Msa0139340:CDS
CCCTCTTGTTATGAAATTTG+GGG 0.553952 1_3:+72999065 Msa0139340:three_prime_UTR
AAGGAGCGAGAAATAGTGGT+GGG 0.557007 1_3:-72996450 None:intergenic
TCAGGGAAGGTCTTTCCGGC+AGG 0.561022 1_3:+72998922 Msa0139340:CDS
CATTTGTTGTAAAATAAGGA+TGG 0.562464 1_3:+72999131 Msa0139340:three_prime_UTR
CTTGTGTCGTTCATAATGAC+CGG 0.562716 1_3:+72998955 Msa0139340:CDS
ACAAGTGACACAACACCTGC+CGG 0.564042 1_3:-72998937 None:intergenic
AGAAATAGTGGTGGGAGTGT+GGG 0.564262 1_3:-72996442 None:intergenic
GGTTGTAGGGCGAAGAGAGA+GGG 0.564448 1_3:-72996415 None:intergenic
ATGACCGGTGGTTACTTGCA+TGG 0.564829 1_3:+72998970 Msa0139340:CDS
TCACACTAGAATGTAAATAA+CGG 0.564961 1_3:+72999006 Msa0139340:exon
ACCCAGCGAAACAGATTGAG+AGG 0.567492 1_3:+72999279 Msa0139340:three_prime_UTR
GTAGGGCGAAGAGAGAGGGA+AGG 0.571612 1_3:-72996411 None:intergenic
GAATTGGAAAGAGACAAGTA+AGG 0.577001 1_3:-72996495 None:intergenic
ACCCCAAATTTCATAACAAG+AGG 0.577194 1_3:-72999066 None:intergenic
ATGTCGCAGAAATTAACTCT+AGG 0.581649 1_3:+72996738 Msa0139340:CDS
CCCCAAATTTCATAACAAGA+GGG 0.582804 1_3:-72999065 None:intergenic
TTTATGACAAACATAAACCA+TGG 0.585195 1_3:-72999102 None:intergenic
TGACGGTGATAAGAGGAAGA+TGG 0.587068 1_3:+72996707 Msa0139340:CDS
CCCAAATTTCATAACAAGAG+GGG 0.587441 1_3:-72999064 None:intergenic
AATTGGAAAGAGACAAGTAA+GGG 0.591100 1_3:-72996494 None:intergenic
GATTTAGACATTACCGGCGG+CGG 0.591983 1_3:+72996582 Msa0139340:CDS
TTATGACAAACATAAACCAT+GGG 0.603493 1_3:-72999101 None:intergenic
CGCCGGTAATGTCTAAATCG+GGG 0.604362 1_3:-72996578 None:intergenic
GGGAAGAAGCAGGAGAAGCA+AGG 0.608774 1_3:-72996473 None:intergenic
AGTTCCAAAAGCATATGCAT+AGG 0.623095 1_3:-72999222 None:intergenic
GGCGGCGGCGGTGATATCAA+AGG 0.624581 1_3:+72996597 Msa0139340:CDS
GTTCTCGTTTCAAGAAATCA+GGG 0.628947 1_3:+72998905 Msa0139340:CDS
TCGTTTCAAGAAATCAGGGA+AGG 0.630412 1_3:+72998909 Msa0139340:CDS
GCCGGTAATGTCTAAATCGG+GGG 0.634119 1_3:-72996577 None:intergenic
GTAATGGGCTTTCTGAAGAG+TGG 0.639725 1_3:+72997934 Msa0139340:CDS
AATTCCATGCAAGTAACCAC+CGG 0.644415 1_3:-72998974 None:intergenic
AAAGGCGAGGAAGGATCTGA+CGG 0.645896 1_3:+72996690 Msa0139340:CDS
AGACGATAGTAACAAAGGCG+AGG 0.650186 1_3:+72996677 Msa0139340:CDS
GAAGGAGCGAGAAATAGTGG+TGG 0.655482 1_3:-72996451 None:intergenic
CCCGATTTAGACATTACCGG+CGG 0.658939 1_3:+72996579 Msa0139340:CDS
GCATAGGTTCAAACACAACC+GGG 0.658942 1_3:-72999206 None:intergenic
GGAGGAGACGATAGTAACAA+AGG 0.659434 1_3:+72996672 Msa0139340:CDS
GGGTTGTAGGGCGAAGAGAG+AGG 0.659984 1_3:-72996416 None:intergenic
GTGTCGTTCATAATGACCGG+TGG 0.667012 1_3:+72998958 Msa0139340:CDS
CCTTACAAAAGACGAAAGAG+TGG 0.672058 1_3:+72996274 Msa0139340:five_prime_UTR
GTCAATGGCAGAAACTGCAG+CGG 0.675091 1_3:+72996362 Msa0139340:exon
ATTGGAAAGAGACAAGTAAG+GGG 0.676028 1_3:-72996493 None:intergenic
GTGGCCCAATACAGAAACCA+CGG 0.696688 1_3:+72996293 Msa0139340:five_prime_UTR
GGAAACGGAGACGATTTCGG+CGG 0.728758 1_3:+72996618 Msa0139340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAAAATTATTTAACA+TGG + chr1_3:72998219-72998238 Msa0139340:intron 10.0%
!!! TTTATTTTGGAATATTATTT+TGG + chr1_3:72998680-72998699 Msa0139340:intron 10.0%
!! TTTCTTATGTTAGTTATTTA+AGG + chr1_3:72996965-72996984 Msa0139340:intron 15.0%
!! TCTACATAATGATATTTATT+TGG + chr1_3:72997893-72997912 Msa0139340:intron 15.0%
!! CTACATAATGATATTTATTT+GGG + chr1_3:72997894-72997913 Msa0139340:intron 15.0%
!! AAAAAATTATTTAACATGGA+TGG + chr1_3:72998223-72998242 Msa0139340:intron 15.0%
!! TAATATTCCAAAATAAATTC+AGG - chr1_3:72998677-72998696 None:intergenic 15.0%
!! GAAATAAAATCTCACAAAAA+CGG + chr1_3:72997369-72997388 Msa0139340:intron 20.0%
!! ATAGATAAGTGTTTATAAGT+GGG - chr1_3:72997415-72997434 None:intergenic 20.0%
!! ATAAACTGGAATAAATTGTA+TGG + chr1_3:72997573-72997592 Msa0139340:intron 20.0%
!! GATATTTGACAACATTAAAT+TGG + chr1_3:72997699-72997718 Msa0139340:intron 20.0%
!!! AGTTTTTTATCCATACTTTA+AGG + chr1_3:72998411-72998430 Msa0139340:intron 20.0%
!!! TTCTAAAAAACCTTAAAGTA+TGG - chr1_3:72998424-72998443 None:intergenic 20.0%
!!! TAGACATTTGTTGTAAAATA+AGG + chr1_3:72999127-72999146 Msa0139340:three_prime_UTR 20.0%
! TAGTTATAGATCAGTTTCTA+GGG - chr1_3:72996831-72996850 None:intergenic 25.0%
! TTAGTTATAGATCAGTTTCT+AGG - chr1_3:72996832-72996851 None:intergenic 25.0%
!!! GTTTTGAATTTACCTTCAAT+TGG + chr1_3:72996865-72996884 Msa0139340:intron 25.0%
! AATTCCTCCATATAATATTG+CGG + chr1_3:72997165-72997184 Msa0139340:intron 25.0%
! TAGATAAGTGTTTATAAGTG+GGG - chr1_3:72997414-72997433 None:intergenic 25.0%
! CATAGATAAGTGTTTATAAG+TGG - chr1_3:72997416-72997435 None:intergenic 25.0%
!! TGAATAAACTCTTTTACTGT+TGG + chr1_3:72998122-72998141 Msa0139340:intron 25.0%
!! GAATAAACTCTTTTACTGTT+GGG + chr1_3:72998123-72998142 Msa0139340:intron 25.0%
! TCTTAAAGTCTTAAGAACAA+AGG + chr1_3:72998176-72998195 Msa0139340:intron 25.0%
! ATTATCGTCGTTATCAAATA+TGG - chr1_3:72998613-72998632 None:intergenic 25.0%
! TCACACTAGAATGTAAATAA+CGG + chr1_3:72999006-72999025 Msa0139340:exon 25.0%
! TTATGACAAACATAAACCAT+GGG - chr1_3:72999104-72999123 None:intergenic 25.0%
! TTTATGACAAACATAAACCA+TGG - chr1_3:72999105-72999124 None:intergenic 25.0%
! ATGGTTTATGTTTGTCATAA+AGG + chr1_3:72999104-72999123 Msa0139340:three_prime_UTR 25.0%
!!! CATTTGTTGTAAAATAAGGA+TGG + chr1_3:72999131-72999150 Msa0139340:three_prime_UTR 25.0%
AATTGGAAAGAGACAAGTAA+GGG - chr1_3:72996497-72996516 None:intergenic 30.0%
AGTTAGAATGAAGAACATGT+TGG + chr1_3:72996891-72996910 Msa0139340:intron 30.0%
AGATAAGTGTTTATAAGTGG+GGG - chr1_3:72997413-72997432 None:intergenic 30.0%
!!! AACATGTTTTGTTTGTGTTG+TGG + chr1_3:72997539-72997558 Msa0139340:intron 30.0%
!!! ACATGTTTTGTTTGTGTTGT+GGG + chr1_3:72997540-72997559 Msa0139340:intron 30.0%
AATTGTATGGAAATTCCATG+TGG + chr1_3:72997586-72997605 Msa0139340:intron 30.0%
ATTGTATGGAAATTCCATGT+GGG + chr1_3:72997587-72997606 Msa0139340:intron 30.0%
!! ATTTATTTGGGTTTTGACAG+CGG + chr1_3:72997906-72997925 Msa0139340:intron 30.0%
GTAAAAGAGTTTATTCAGAC+AGG - chr1_3:72998119-72998138 None:intergenic 30.0%
GGAATTTCATATGTATCAGA+AGG + chr1_3:72998197-72998216 Msa0139340:intron 30.0%
! GTGAATTGTAAAGCATTCTT+TGG + chr1_3:72998347-72998366 Msa0139340:intron 30.0%
AATATGAAGTGTAGAGTTCA+GGG - chr1_3:72998377-72998396 None:intergenic 30.0%
TAATATGAAGTGTAGAGTTC+AGG - chr1_3:72998378-72998397 None:intergenic 30.0%
TATGTTGCAAATTGTTCAAC+TGG + chr1_3:72998474-72998493 Msa0139340:intron 30.0%
!! GTTTCTTTGCTTTGAAGTTT+AGG + chr1_3:72998576-72998595 Msa0139340:intron 30.0%
GCAAACTCTTTATGAGTTTA+CGG + chr1_3:72998709-72998728 Msa0139340:intron 30.0%
AAACATTAACACATGAGAGA+AGG - chr1_3:72998833-72998852 None:intergenic 30.0%
!! GATCATTTTTCCTGATGTAT+AGG + chr1_3:72998853-72998872 Msa0139340:intron 30.0%
TTGTATTCAGCAAAGTTTAC+AGG - chr1_3:72999043-72999062 None:intergenic 30.0%
! GGTAAATGTGAACTTTTGTT+TGG + chr1_3:72999152-72999171 Msa0139340:three_prime_UTR 30.0%
!!! ATGTTGCTCTTTTTTATTCC+CGG + chr1_3:72999188-72999207 Msa0139340:three_prime_UTR 30.0%
! TCACTGGAAGAAGATTCTTT+TGG - chr1_3:72996321-72996340 None:intergenic 35.0%
ATTGGAAAGAGACAAGTAAG+GGG - chr1_3:72996496-72996515 None:intergenic 35.0%
GAATTGGAAAGAGACAAGTA+AGG - chr1_3:72996498-72996517 None:intergenic 35.0%
!!! TAGGTCAAATTGACTGGTTT+TGG - chr1_3:72996553-72996572 None:intergenic 35.0%
ATGTCGCAGAAATTAACTCT+AGG + chr1_3:72996738-72996757 Msa0139340:CDS 35.0%
ATTCTAACTCAGCCAATTGA+AGG - chr1_3:72996880-72996899 None:intergenic 35.0%
GAGAAGACTGATACAACTAT+TGG - chr1_3:72997129-72997148 None:intergenic 35.0%
TGTATCAGTCTTCTCAATAG+TGG + chr1_3:72997134-72997153 Msa0139340:intron 35.0%
! GGCCATAAATTTTGAACAGT+GGG + chr1_3:72997219-72997238 Msa0139340:intron 35.0%
! GCCATAAATTTTGAACAGTG+GGG + chr1_3:72997220-72997239 Msa0139340:intron 35.0%
TCCCCACTGTTCAAAATTTA+TGG - chr1_3:72997224-72997243 None:intergenic 35.0%
! ACCATTGCACGAAATTTGTT+TGG + chr1_3:72997244-72997263 Msa0139340:intron 35.0%
TCCAAACAAATTTCGTGCAA+TGG - chr1_3:72997248-72997267 None:intergenic 35.0%
! ATTGCACGAAATTTGTTTGG+AGG + chr1_3:72997247-72997266 Msa0139340:intron 35.0%
! TTGCACGAAATTTGTTTGGA+GGG + chr1_3:72997248-72997267 Msa0139340:intron 35.0%
!! TTTTTCGATTCCATGAAACG+AGG + chr1_3:72997288-72997307 Msa0139340:intron 35.0%
AGACTCTTTAACAGATAGTG+AGG + chr1_3:72997455-72997474 Msa0139340:intron 35.0%
!!! CATGTTTTGTTTGTGTTGTG+GGG + chr1_3:72997541-72997560 Msa0139340:intron 35.0%
!!! ATGTTTTGTTTGTGTTGTGG+GGG + chr1_3:72997542-72997561 Msa0139340:intron 35.0%
TGGGGGAAATCAAAATAAAC+TGG + chr1_3:72997559-72997578 Msa0139340:intron 35.0%
GATGTCAAATATCTTTGTGG+AGG + chr1_3:72997630-72997649 Msa0139340:intron 35.0%
!! TTTATTTGGGTTTTGACAGC+GGG + chr1_3:72997907-72997926 Msa0139340:intron 35.0%
ACATAGCCATGTCTAAATTC+TGG + chr1_3:72998311-72998330 Msa0139340:intron 35.0%
ATATGAAGTGTAGAGTTCAG+GGG - chr1_3:72998376-72998395 None:intergenic 35.0%
TAGAACCTACCATTTACCAA+AGG + chr1_3:72998439-72998458 Msa0139340:intron 35.0%
CATAATTCGTTTGATGCCTT+TGG - chr1_3:72998458-72998477 None:intergenic 35.0%
GCAAATTGTTCAACTGGTTA+TGG + chr1_3:72998480-72998499 Msa0139340:intron 35.0%
ACAATGATGCATCATTCCAT+TGG + chr1_3:72998508-72998527 Msa0139340:intron 35.0%
! CTCGCTTCCTGAATTTATTT+TGG + chr1_3:72998667-72998686 Msa0139340:intron 35.0%
! ATCAATTCTAGTTGCTCACA+AGG - chr1_3:72998779-72998798 None:intergenic 35.0%
GTTCTCGTTTCAAGAAATCA+GGG + chr1_3:72998905-72998924 Msa0139340:CDS 35.0%
! TCCCCTCTTGTTATGAAATT+TGG + chr1_3:72999063-72999082 Msa0139340:three_prime_UTR 35.0%
CCCAAATTTCATAACAAGAG+GGG - chr1_3:72999067-72999086 None:intergenic 35.0%
! CCCCTCTTGTTATGAAATTT+GGG + chr1_3:72999064-72999083 Msa0139340:three_prime_UTR 35.0%
CCCCAAATTTCATAACAAGA+GGG - chr1_3:72999068-72999087 None:intergenic 35.0%
! CCCTCTTGTTATGAAATTTG+GGG + chr1_3:72999065-72999084 Msa0139340:three_prime_UTR 35.0%
ACCCCAAATTTCATAACAAG+AGG - chr1_3:72999069-72999088 None:intergenic 35.0%
! TGAACCTATGCATATGCTTT+TGG + chr1_3:72999218-72999237 Msa0139340:three_prime_UTR 35.0%
! AGTTCCAAAAGCATATGCAT+AGG - chr1_3:72999225-72999244 None:intergenic 35.0%
!!! ATGCATATGCTTTTGGAACT+TGG + chr1_3:72999225-72999244 Msa0139340:three_prime_UTR 35.0%
! CCACTCTTTCGTCTTTTGTA+AGG - chr1_3:72996277-72996296 None:intergenic 40.0%
CCTTACAAAAGACGAAAGAG+TGG + chr1_3:72996274-72996293 Msa0139340:five_prime_UTR 40.0%
TTTCTGCCATTGACGAAAGA+AGG - chr1_3:72996356-72996375 None:intergenic 40.0%
! AGCGAGTTGAGAATGTGAAT+TGG - chr1_3:72996514-72996533 None:intergenic 40.0%
AAAGGAAACGGAGACGATTT+CGG + chr1_3:72996615-72996634 Msa0139340:CDS 40.0%
CTAGGTTATGCTATTCTCGT+TGG + chr1_3:72996756-72996775 Msa0139340:CDS 40.0%
AGTGCCGCAATATTATATGG+AGG - chr1_3:72997172-72997191 None:intergenic 40.0%
! GGGCCATAAATTTTGAACAG+TGG + chr1_3:72997218-72997237 Msa0139340:intron 40.0%
! AATTTGTTTGGAGGGAGAGT+TGG + chr1_3:72997256-72997275 Msa0139340:intron 40.0%
AGAGAGAAAGAGATGATCGT+AGG + chr1_3:72997338-72997357 Msa0139340:intron 40.0%
AGAAAGAGATGATCGTAGGT+GGG + chr1_3:72997342-72997361 Msa0139340:intron 40.0%
TCTGTTAAAGAGTCTCACCT+CGG - chr1_3:72997450-72997469 None:intergenic 40.0%
TCTTTAACAGATAGTGAGGC+AGG + chr1_3:72997459-72997478 Msa0139340:intron 40.0%
CTTTAACAGATAGTGAGGCA+GGG + chr1_3:72997460-72997479 Msa0139340:intron 40.0%
GGGGATGTCAAATATCTTTG+TGG + chr1_3:72997627-72997646 Msa0139340:intron 40.0%
TCAAATATCTTTGTGGAGGC+AGG + chr1_3:72997634-72997653 Msa0139340:intron 40.0%
!! TATGTTTTTACCGAGCTACC+CGG + chr1_3:72998006-72998025 Msa0139340:CDS 40.0%
TAGCCATGTCTAAATTCTGG+AGG + chr1_3:72998314-72998333 Msa0139340:intron 40.0%
CAACCTCCAGAATTTAGACA+TGG - chr1_3:72998320-72998339 None:intergenic 40.0%
TGATGCCTTTGGTAAATGGT+AGG - chr1_3:72998447-72998466 None:intergenic 40.0%
CGTTTGATGCCTTTGGTAAA+TGG - chr1_3:72998451-72998470 None:intergenic 40.0%
AGAGCACAATTGTGTACCAA+TGG - chr1_3:72998527-72998546 None:intergenic 40.0%
TGGTACACAATTGTGCTCTT+TGG + chr1_3:72998528-72998547 Msa0139340:intron 40.0%
GAGCTAGAAAAATTCCACAC+TGG - chr1_3:72998650-72998669 None:intergenic 40.0%
AGCAGATATGCCTATACATC+AGG - chr1_3:72998866-72998885 None:intergenic 40.0%
GGTTCTCGTTTCAAGAAATC+AGG + chr1_3:72998904-72998923 Msa0139340:CDS 40.0%
TCGTTTCAAGAAATCAGGGA+AGG + chr1_3:72998909-72998928 Msa0139340:CDS 40.0%
! CTTGTGTCGTTCATAATGAC+CGG + chr1_3:72998955-72998974 Msa0139340:CDS 40.0%
AATTCCATGCAAGTAACCAC+CGG - chr1_3:72998977-72998996 None:intergenic 40.0%
TGCATAGGTTCAAACACAAC+CGG - chr1_3:72999210-72999229 None:intergenic 40.0%
!!! AAGGTTCTGGTTTTGCTCAC+TGG - chr1_3:72996337-72996356 None:intergenic 45.0%
CCATTGACGAAAGAAGGTTC+TGG - chr1_3:72996350-72996369 None:intergenic 45.0%
CCAGAACCTTCTTTCGTCAA+TGG + chr1_3:72996347-72996366 Msa0139340:exon 45.0%
! AGAAATAGTGGTGGGAGTGT+GGG - chr1_3:72996445-72996464 None:intergenic 45.0%
AAGGAGCGAGAAATAGTGGT+GGG - chr1_3:72996453-72996472 None:intergenic 45.0%
AAGGAAGGAGCGAGAAATAG+TGG - chr1_3:72996457-72996476 None:intergenic 45.0%
GGCACTTAGGTCAAATTGAC+TGG - chr1_3:72996559-72996578 None:intergenic 45.0%
GGCGGTGATATCAAAGGAAA+CGG + chr1_3:72996603-72996622 Msa0139340:CDS 45.0%
GGAGGAGACGATAGTAACAA+AGG + chr1_3:72996672-72996691 Msa0139340:CDS 45.0%
AGACGATAGTAACAAAGGCG+AGG + chr1_3:72996677-72996696 Msa0139340:CDS 45.0%
GATAGTAACAAAGGCGAGGA+AGG + chr1_3:72996681-72996700 Msa0139340:CDS 45.0%
AAGGATCTGACGGTGATAAG+AGG + chr1_3:72996700-72996719 Msa0139340:CDS 45.0%
TGACGGTGATAAGAGGAAGA+TGG + chr1_3:72996707-72996726 Msa0139340:CDS 45.0%
GGTTATGCTATTCTCGTTGG+AGG + chr1_3:72996759-72996778 Msa0139340:CDS 45.0%
GGCAGTGCCGCAATATTATA+TGG - chr1_3:72997175-72997194 None:intergenic 45.0%
CACTGCCATCCTTCACTAAT+TGG + chr1_3:72997188-72997207 Msa0139340:intron 45.0%
TGCCATCCTTCACTAATTGG+CGG + chr1_3:72997191-72997210 Msa0139340:intron 45.0%
TCGATTCCATGAAACGAGGA+AGG + chr1_3:72997292-72997311 Msa0139340:intron 45.0%
CGATTCCATGAAACGAGGAA+GGG + chr1_3:72997293-72997312 Msa0139340:intron 45.0%
GAAAACCCTTCCTCGTTTCA+TGG - chr1_3:72997301-72997320 None:intergenic 45.0%
GAGAAAGAGATGATCGTAGG+TGG + chr1_3:72997341-72997360 Msa0139340:intron 45.0%
TCTCACAAAAACGGCATGTG+AGG + chr1_3:72997378-72997397 Msa0139340:intron 45.0%
TCTATGTCATCTCTCAACCG+AGG + chr1_3:72997430-72997449 Msa0139340:intron 45.0%
TCCATGTGGGTAGACAAGTA+TGG + chr1_3:72997600-72997619 Msa0139340:intron 45.0%
ACCATACTTGTCTACCCACA+TGG - chr1_3:72997604-72997623 None:intergenic 45.0%
TGTGGGTAGACAAGTATGGT+AGG + chr1_3:72997604-72997623 Msa0139340:intron 45.0%
GTGGGTAGACAAGTATGGTA+GGG + chr1_3:72997605-72997624 Msa0139340:intron 45.0%
TGGGTAGACAAGTATGGTAG+GGG + chr1_3:72997606-72997625 Msa0139340:intron 45.0%
TCAAATATCTGCCACCACAG+TGG - chr1_3:72997688-72997707 None:intergenic 45.0%
!! GTAATGGGCTTTCTGAAGAG+TGG + chr1_3:72997934-72997953 Msa0139340:CDS 45.0%
TGATGCAAGAACAGGTCTTC+CGG - chr1_3:72998028-72998047 None:intergenic 45.0%
ACCTGTTCTTGCATCATCTG+TGG + chr1_3:72998032-72998051 Msa0139340:CDS 45.0%
CCTGTTCTTGCATCATCTGT+GGG + chr1_3:72998033-72998052 Msa0139340:CDS 45.0%
TGGCTATGTTTCAGCCTTAG+CGG - chr1_3:72998300-72998319 None:intergenic 45.0%
! TTTAGCACTGAAGTCCAGTG+TGG + chr1_3:72998633-72998652 Msa0139340:intron 45.0%
CATATCTGCTGCACTTCTTG+TGG + chr1_3:72998876-72998895 Msa0139340:CDS 45.0%
GAAATCAGGGAAGGTCTTTC+CGG + chr1_3:72998918-72998937 Msa0139340:CDS 45.0%
GCATAGGTTCAAACACAACC+GGG - chr1_3:72999209-72999228 None:intergenic 45.0%
TCCTCTCAATCTGTTTCGCT+GGG - chr1_3:72999283-72999302 None:intergenic 45.0%
GTGGCCCAATACAGAAACCA+CGG + chr1_3:72996293-72996312 Msa0139340:five_prime_UTR 50.0%
! AGAAGATTCTTTTGGCGCCG+TGG - chr1_3:72996313-72996332 None:intergenic 50.0%
GTCAATGGCAGAAACTGCAG+CGG + chr1_3:72996362-72996381 Msa0139340:exon 50.0%
! GCAGAAACTGCAGCGGTTTT+CGG + chr1_3:72996369-72996388 Msa0139340:CDS 50.0%
! GAGAAATAGTGGTGGGAGTG+TGG - chr1_3:72996446-72996465 None:intergenic 50.0%
GAAGGAGCGAGAAATAGTGG+TGG - chr1_3:72996454-72996473 None:intergenic 50.0%
AGAAGCAGGAGAAGCAAGGA+AGG - chr1_3:72996472-72996491 None:intergenic 50.0%
GACAAGTAAGGGGAAGAAGC+AGG - chr1_3:72996486-72996505 None:intergenic 50.0%
!! TGTCTAAATCGGGGGCACTT+AGG - chr1_3:72996572-72996591 None:intergenic 50.0%
GCCCCCGATTTAGACATTAC+CGG + chr1_3:72996576-72996595 Msa0139340:CDS 50.0%
GCCGGTAATGTCTAAATCGG+GGG - chr1_3:72996580-72996599 None:intergenic 50.0%
CGCCGGTAATGTCTAAATCG+GGG - chr1_3:72996581-72996600 None:intergenic 50.0%
CCGCCGGTAATGTCTAAATC+GGG - chr1_3:72996582-72996601 None:intergenic 50.0%
CCCGATTTAGACATTACCGG+CGG + chr1_3:72996579-72996598 Msa0139340:CDS 50.0%
GCCGCCGGTAATGTCTAAAT+CGG - chr1_3:72996583-72996602 None:intergenic 50.0%
GATTTAGACATTACCGGCGG+CGG + chr1_3:72996582-72996601 Msa0139340:CDS 50.0%
AAAGGCGAGGAAGGATCTGA+CGG + chr1_3:72996690-72996709 Msa0139340:CDS 50.0%
CACCGCCAATTAGTGAAGGA+TGG - chr1_3:72997196-72997215 None:intergenic 50.0%
CTTCACTAATTGGCGGTGCA+GGG + chr1_3:72997198-72997217 Msa0139340:intron 50.0%
GGGTAGACAAGTATGGTAGG+GGG + chr1_3:72997607-72997626 Msa0139340:intron 50.0%
GGTAGACAAGTATGGTAGGG+GGG + chr1_3:72997608-72997627 Msa0139340:intron 50.0%
AGCCAGACACAAGGTTGTGT+GGG - chr1_3:72997662-72997681 None:intergenic 50.0%
AATATCTGCCACCACAGTGG+TGG - chr1_3:72997685-72997704 None:intergenic 50.0%
!! ATTTGGGTTTTGACAGCGGG+TGG + chr1_3:72997910-72997929 Msa0139340:intron 50.0%
TTTGACAGCGGGTGGTGTAA+TGG + chr1_3:72997918-72997937 Msa0139340:intron 50.0%
TTGACAGCGGGTGGTGTAAT+GGG + chr1_3:72997919-72997938 Msa0139340:intron 50.0%
!! TGGCAGCCAGAAGTCACTTT+TGG + chr1_3:72997954-72997973 Msa0139340:CDS 50.0%
!!! AGCCAGAAGTCACTTTTGGC+TGG + chr1_3:72997958-72997977 Msa0139340:CDS 50.0%
!!! GCCAGAAGTCACTTTTGGCT+GGG + chr1_3:72997959-72997978 Msa0139340:CDS 50.0%
!!! CCAGAAGTCACTTTTGGCTG+GGG + chr1_3:72997960-72997979 Msa0139340:CDS 50.0%
!!! CAGAAGTCACTTTTGGCTGG+GGG + chr1_3:72997961-72997980 Msa0139340:CDS 50.0%
GATGCAAGAACAGGTCTTCC+GGG - chr1_3:72998027-72998046 None:intergenic 50.0%
CCCACAGATGATGCAAGAAC+AGG - chr1_3:72998036-72998055 None:intergenic 50.0%
AGCTCAGCAGTGACTTACCA+AGG - chr1_3:72998059-72998078 None:intergenic 50.0%
TCTTGAGTTTCTGCCCGCTA+AGG + chr1_3:72998283-72998302 Msa0139340:intron 50.0%
GGCTATGTTTCAGCCTTAGC+GGG - chr1_3:72998299-72998318 None:intergenic 50.0%
TGCTGCACTTCTTGTGGTGA+TGG + chr1_3:72998882-72998901 Msa0139340:CDS 50.0%
GCTGCACTTCTTGTGGTGAT+GGG + chr1_3:72998883-72998902 Msa0139340:CDS 50.0%
ACAAGTGACACAACACCTGC+CGG - chr1_3:72998940-72998959 None:intergenic 50.0%
! GTGTCGTTCATAATGACCGG+TGG + chr1_3:72998958-72998977 Msa0139340:CDS 50.0%
ATGACCGGTGGTTACTTGCA+TGG + chr1_3:72998970-72998989 Msa0139340:CDS 50.0%
ACCCAGCGAAACAGATTGAG+AGG + chr1_3:72999279-72999298 Msa0139340:three_prime_UTR 50.0%
CTCCTCTCAATCTGTTTCGC+TGG - chr1_3:72999284-72999303 None:intergenic 50.0%
GGCGCCGTGGTTTCTGTATT+GGG - chr1_3:72996300-72996319 None:intergenic 55.0%
TGGCGCCGTGGTTTCTGTAT+TGG - chr1_3:72996301-72996320 None:intergenic 55.0%
GGTTGTAGGGCGAAGAGAGA+GGG - chr1_3:72996418-72996437 None:intergenic 55.0%
! GAGTGTGGGATCGGGTTGTA+GGG - chr1_3:72996431-72996450 None:intergenic 55.0%
! TAGTGGTGGGAGTGTGGGAT+CGG - chr1_3:72996440-72996459 None:intergenic 55.0%
GGGAAGAAGCAGGAGAAGCA+AGG - chr1_3:72996476-72996495 None:intergenic 55.0%
GGAAACGGAGACGATTTCGG+CGG + chr1_3:72996618-72996637 Msa0139340:CDS 55.0%
CCTGCACCGCCAATTAGTGA+AGG - chr1_3:72997200-72997219 None:intergenic 55.0%
CCTTCACTAATTGGCGGTGC+AGG + chr1_3:72997197-72997216 Msa0139340:intron 55.0%
!! GTTTGGAGGGAGAGTTGGTG+TGG + chr1_3:72997261-72997280 Msa0139340:intron 55.0%
AGCCCACACAACCTTGTGTC+TGG + chr1_3:72997657-72997676 Msa0139340:intron 55.0%
GAGCCAGACACAAGGTTGTG+TGG - chr1_3:72997663-72997682 None:intergenic 55.0%
CCCCAGCCAAAAGTGACTTC+TGG - chr1_3:72997963-72997982 None:intergenic 55.0%
AACAGGTCTTCCGGGTAGCT+CGG - chr1_3:72998019-72998038 None:intergenic 55.0%
CTTGCATCATCTGTGGGCCT+TGG + chr1_3:72998039-72998058 Msa0139340:CDS 55.0%
GGGTTTGTGCTGTAGACCCA+TGG + chr1_3:72999085-72999104 Msa0139340:three_prime_UTR 55.0%
GTAGGGCGAAGAGAGAGGGA+AGG - chr1_3:72996414-72996433 None:intergenic 60.0%
GGGTTGTAGGGCGAAGAGAG+AGG - chr1_3:72996419-72996438 None:intergenic 60.0%
! GGAGTGTGGGATCGGGTTGT+AGG - chr1_3:72996432-72996451 None:intergenic 60.0%
! AGTGGTGGGAGTGTGGGATC+GGG - chr1_3:72996439-72996458 None:intergenic 60.0%
TTAGACATTACCGGCGGCGG+CGG + chr1_3:72996585-72996604 Msa0139340:CDS 60.0%
TTTGATATCACCGCCGCCGC+CGG - chr1_3:72996598-72996617 None:intergenic 60.0%
AACGGAGACGATTTCGGCGG+AGG + chr1_3:72996621-72996640 Msa0139340:CDS 60.0%
! CAGTGGTGGAGCCAGACACA+AGG - chr1_3:72997671-72997690 None:intergenic 60.0%
! TGTGTCTGGCTCCACCACTG+TGG + chr1_3:72997671-72997690 Msa0139340:intron 60.0%
TCAGGGAAGGTCTTTCCGGC+AGG + chr1_3:72998922-72998941 Msa0139340:CDS 60.0%
GGCGGCGGCGGTGATATCAA+AGG + chr1_3:72996597-72996616 Msa0139340:CDS 65.0%
GGAGACGATTTCGGCGGAGG+AGG + chr1_3:72996624-72996643 Msa0139340:CDS 65.0%
GACGATTTCGGCGGAGGAGG+TGG + chr1_3:72996627-72996646 Msa0139340:CDS 65.0%
GATTTCGGCGGAGGAGGTGG+TGG + chr1_3:72996630-72996649 Msa0139340:CDS 65.0%
!! GGAGGTGGTGGTGGTGGTGA+AGG + chr1_3:72996642-72996661 Msa0139340:CDS 65.0%
!! GGTGGTGGTGGTGGTGAAGG+AGG + chr1_3:72996645-72996664 Msa0139340:CDS 65.0%
!! GGTGGTGGTGGTGAAGGAGG+AGG + chr1_3:72996648-72996667 Msa0139340:CDS 65.0%
! GGTGGTGGTGAAGGAGGAGG+CGG + chr1_3:72996651-72996670 Msa0139340:CDS 65.0%
! GTCTGGCTCCACCACTGTGG+TGG + chr1_3:72997674-72997693 Msa0139340:intron 65.0%
! TTCGGCGGAGGAGGTGGTGG+TGG + chr1_3:72996633-72996652 Msa0139340:CDS 70.0%
GGTGGTGAAGGAGGAGGCGG+AGG + chr1_3:72996654-72996673 Msa0139340:CDS 70.0%
!! GGCGGAGGAGGTGGTGGTGG+TGG + chr1_3:72996636-72996655 Msa0139340:CDS 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 72996261 72999313 72996261 ID=Msa0139340;Name=Msa0139340
chr1_3 mRNA 72996261 72999313 72996261 ID=Msa0139340-mRNA-1;Parent=Msa0139340;Name=Msa0139340-mRNA-1;_AED=0.00;_eAED=0.00;_QI=105|1|1|1|1|1|3|299|229
chr1_3 exon 72996261 72996780 72996261 ID=Msa0139340-mRNA-1:exon:25438;Parent=Msa0139340-mRNA-1
chr1_3 exon 72997926 72998060 72997926 ID=Msa0139340-mRNA-1:exon:25439;Parent=Msa0139340-mRNA-1
chr1_3 exon 72998875 72999313 72998875 ID=Msa0139340-mRNA-1:exon:25440;Parent=Msa0139340-mRNA-1
chr1_3 five_prime_UTR 72996261 72996365 72996261 ID=Msa0139340-mRNA-1:five_prime_utr;Parent=Msa0139340-mRNA-1
chr1_3 CDS 72996366 72996780 72996366 ID=Msa0139340-mRNA-1:cds;Parent=Msa0139340-mRNA-1
chr1_3 CDS 72997926 72998060 72997926 ID=Msa0139340-mRNA-1:cds;Parent=Msa0139340-mRNA-1
chr1_3 CDS 72998875 72999014 72998875 ID=Msa0139340-mRNA-1:cds;Parent=Msa0139340-mRNA-1
chr1_3 three_prime_UTR 72999015 72999313 72999015 ID=Msa0139340-mRNA-1:three_prime_utr;Parent=Msa0139340-mRNA-1
Gene Sequence

>Msa0139340

ATGGCAGAAACTGCAGCGGTTTTCGGTGTTTGCAGTTCAACACTTCCTTCCCTCTCTCTTCGCCCTACAACCCGATCCCACACTCCCACCACTATTTCTCGCTCCTTCCTTGCTTCTCCTGCTTCTTCCCCTTACTTGTCTCTTTCCAATTCACATTCTCAACTCGCTGTTGTTTCTTCTGATTCCAAAACCAGTCAATTTGACCTAAGTGCCCCCGATTTAGACATTACCGGCGGCGGCGGTGATATCAAAGGAAACGGAGACGATTTCGGCGGAGGAGGTGGTGGTGGTGGTGAAGGAGGAGGCGGAGGAGACGATAGTAACAAAGGCGAGGAAGGATCTGACGGTGATAAGAGGAAGATGGCTTTGTCAATGTCGCAGAAATTAACTCTAGGTTATGCTATTCTCGTTGGAGCGGGTGGTGTAATGGGCTTTCTGAAGAGTGGCAGCCAGAAGTCACTTTTGGCTGGGGGTTTGTCTTCTGCACTGCTGTTTTATGTTTTTACCGAGCTACCCGGAAGACCTGTTCTTGCATCATCTGTGGGCCTTGGCATATCTGCTGCACTTCTTGTGGTGATGGGTTCTCGTTTCAAGAAATCAGGGAAGGTCTTTCCGGCAGGTGTTGTGTCACTTGTGTCGTTCATAATGACCGGTGGTTACTTGCATGGAATTATGCGTAGTTCACACTAG
Protein sequence

>Msa0139340

MAETAAVFGVCSSTLPSLSLRPTTRSHTPTTISRSFLASPASSPYLSLSNSHSQLAVVSSDSKTSQFDLSAPDLDITGGGGDIKGNGDDFGGGGGGGGEGGGGGDDSNKGEEGSDGDKRKMALSMSQKLTLGYAILVGAGGVMGFLKSGSQKSLLAGGLSSALLFYVFTELPGRPVLASSVGLGISAALLVVMGSRFKKSGKVFPAGVVSLVSFIMTGGYLHGIMRSSH*