AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1183630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1183630 MtrunA17_Chr8g0335791 100.000 149 0 0 1 149 1 149 3.10e-108 304
Msa1183630 MtrunA17_Chr4g0057901 64.865 148 51 1 3 149 83 230 1.19e-66 202
Msa1183630 MtrunA17_Chr8g0388131 59.184 147 59 1 3 148 85 231 2.88e-62 191
Msa1183630 MtrunA17_Chr8g0344421 34.074 135 86 1 3 134 34 168 2.50e-24 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1183630 AT4G09320.1 83.673 147 24 0 3 149 2 148 1.81e-91 262
Msa1183630 AT5G63310.1 63.514 148 53 1 3 149 84 231 9.57e-67 202
Msa1183630 AT4G11010.1 59.184 147 59 1 3 148 89 235 2.87e-62 191
Msa1183630 AT4G23900.1 59.864 147 58 1 3 148 88 234 3.32e-62 191
Msa1183630 AT4G23895.3 59.864 147 58 1 3 148 318 464 5.81e-60 192
Msa1183630 AT1G17410.1 35.556 135 84 1 3 134 33 167 1.46e-22 88.6
Msa1183630 AT1G17410.2 35.385 130 81 1 8 134 1 130 2.33e-21 84.3
Msa1183630 AT1G17410.3 34.066 91 60 0 8 98 1 91 3.95e-12 60.1

Find 70 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCAGCAAAGGATCAAAAT+AGG 0.237868 8_1:-18915609 None:intergenic
TTAATCTGAATTTGAAGTTT+TGG 0.249353 8_1:+18915716 Msa1183630:three_prime_UTR
GAAAGCGCCAACAAGGAAAT+TGG 0.280936 8_1:+18915490 Msa1183630:CDS
CCCTGTTGTTGCCATGATTT+GGG 0.284074 8_1:+18914760 Msa1183630:CDS
GCCCTGTTGTTGCCATGATT+TGG 0.311298 8_1:+18914759 Msa1183630:CDS
GCCATCTTGATTTCTCTGTT+TGG 0.335715 8_1:-18913525 None:intergenic
GTAAATTCACGATCAACTTC+AGG 0.340570 8_1:-18915586 None:intergenic
CCACAAACCAATTTCCTTGT+TGG 0.361994 8_1:-18915497 None:intergenic
TGTGGTGGTGTGAGAGGAAA+GGG 0.366610 8_1:-18913455 None:intergenic
TATCAGCAGGTTTGAGAAGA+AGG 0.368456 8_1:+18913998 Msa1183630:CDS
TCCCAAATCATGGCAACAAC+AGG 0.371495 8_1:-18914761 None:intergenic
GGTGACTTTGCTGTTGAGAT+TGG 0.374163 8_1:+18914863 Msa1183630:CDS
CCGATAAATTAAAATAATAA+TGG 0.378171 8_1:-18915807 None:intergenic
TATGTGCCGAATATTCGTTT+TGG 0.379484 8_1:+18913385 None:intergenic
GGAAAGTGAAATTAGGGTTA+AGG 0.387235 8_1:+18913407 Msa1183630:five_prime_UTR
TGCCATGCAGCAGGTCCCTC+AGG 0.393989 8_1:-18915523 None:intergenic
AGGCTGCTTTGCCATGCAGC+AGG 0.417205 8_1:-18915532 None:intergenic
TGATGCAGGAACGTTATTCA+TGG 0.430285 8_1:+18915454 Msa1183630:intron
TCTCTGTTTGGTATTGAGTT+CGG 0.433255 8_1:-18913513 None:intergenic
GTTACTGTTGTTGCTGTGTT+GGG 0.433386 8_1:-18913491 None:intergenic
GACAGATCCCTTAGGTTGGT+TGG 0.438255 8_1:+18915946 Msa1183630:three_prime_UTR
CTCAGATTGAGCAGGGTTTG+TGG 0.440349 8_1:-18914825 None:intergenic
TCCAGCAAAGGATCAAAATA+GGG 0.440715 8_1:-18915608 None:intergenic
ACAACTGGCAGAAAGATTAT+TGG 0.443417 8_1:+18914800 Msa1183630:CDS
GGGAAGAATGTTGTGACAAC+TGG 0.448668 8_1:+18914785 Msa1183630:CDS
CCAACAAGGAAATTGGTTTG+TGG 0.455431 8_1:+18915497 Msa1183630:CDS
AAGTCACCACGAATAGTTCC+AGG 0.456893 8_1:-18914848 None:intergenic
GGTTACTGTTGTTGCTGTGT+TGG 0.459069 8_1:-18913492 None:intergenic
GTGACTTTGCTGTTGAGATT+GGG 0.461283 8_1:+18914864 Msa1183630:CDS
ATTCTTCCCTTCCCAAATCA+TGG 0.462620 8_1:-18914771 None:intergenic
TGGTGGTGTGAGAGGAAAGG+GGG 0.468982 8_1:-18913453 None:intergenic
GTGTGGTGGTGTGAGAGGAA+AGG 0.488846 8_1:-18913456 None:intergenic
TTGGTAGAGTACATTGTCTC+TGG 0.492742 8_1:+18914737 Msa1183630:CDS
TCTGAGCCTGGAACTATTCG+TGG 0.495457 8_1:+18914842 Msa1183630:CDS
AGTCCTCTTTGAACACCATC+AGG 0.504010 8_1:-18913573 None:intergenic
TGATAGTTCCAACCAACCTA+AGG 0.504958 8_1:-18915954 None:intergenic
TCTACCAATCCGCTGAAGAA+AGG 0.506686 8_1:-18914722 None:intergenic
AAGAAGGGTTTCTACTTGAA+AGG 0.507643 8_1:+18914014 Msa1183630:CDS
AACCCTGCTCAATCTGAGCC+TGG 0.508261 8_1:+18914830 Msa1183630:CDS
GTTCCAGGCTCAGATTGAGC+AGG 0.508318 8_1:-18914833 None:intergenic
TTTAAGTGCAACCACATCAA+TGG 0.514650 8_1:-18915779 None:intergenic
TTTGCATAGACAGATCCCTT+AGG 0.521901 8_1:+18915938 Msa1183630:three_prime_UTR
TCTGCAAAGCCTTTCTTCAG+CGG 0.530736 8_1:+18914713 Msa1183630:CDS
AAAGCCTTTCTTCAGCGGAT+TGG 0.536682 8_1:+18914718 Msa1183630:CDS
ATCAGCAGGTTTGAGAAGAA+GGG 0.548918 8_1:+18913999 Msa1183630:CDS
ATTGGTTTGTGGTTCCCTGA+GGG 0.548939 8_1:+18915508 Msa1183630:CDS
CATAGACAGATCCCTTAGGT+TGG 0.549764 8_1:+18915942 Msa1183630:three_prime_UTR
GGCAAAGCAGCCTCCACTCG+TGG 0.563561 8_1:+18915542 Msa1183630:CDS
GGTGGTGTGAGAGGAAAGGG+GGG 0.566114 8_1:-18913452 None:intergenic
TTCCAGGCTCAGATTGAGCA+GGG 0.574819 8_1:-18914832 None:intergenic
GGAGAGAGTGTGATGTGTGG+TGG 0.583086 8_1:-18913470 None:intergenic
GCCATGCAGCAGGTCCCTCA+GGG 0.586568 8_1:-18915522 None:intergenic
GATAGTTCCAACCAACCTAA+GGG 0.588934 8_1:-18915953 None:intergenic
AGGTTGGTGAGATTATCAGC+AGG 0.589081 8_1:+18913985 Msa1183630:intron
TTCTGTTGAAAGCGCCAACA+AGG 0.589117 8_1:+18915483 Msa1183630:CDS
GTGATGTGTGGTGGTGTGAG+AGG 0.590503 8_1:-18913461 None:intergenic
TTCATCATGATCAAGCCTGA+TGG 0.593169 8_1:+18913558 Msa1183630:CDS
AACACTCAAAGTTCCAGCAA+AGG 0.595886 8_1:-18915620 None:intergenic
GTTGTTGCCATGATTTGGGA+AGG 0.598008 8_1:+18914764 Msa1183630:CDS
ACTTTGCTGTTGAGATTGGG+AGG 0.598896 8_1:+18914867 Msa1183630:CDS
AAGCCTGATGGTGTTCAAAG+AGG 0.613792 8_1:+18913570 Msa1183630:CDS
ACTCGTAAATCCACGAGTGG+AGG 0.617859 8_1:-18915552 None:intergenic
CCCAAATCATGGCAACAACA+GGG 0.626477 8_1:-18914760 None:intergenic
GTGGTGGTGTGAGAGGAAAG+GGG 0.627876 8_1:-18913454 None:intergenic
ACCAAACAGAGAAATCAAGA+TGG 0.632343 8_1:+18913524 Msa1183630:exon
TTGGGAGAGAGTGTGATGTG+TGG 0.641202 8_1:-18913473 None:intergenic
TTGTTGCCATGATTTGGGAA+GGG 0.646062 8_1:+18914765 Msa1183630:CDS
AATTGGTTTGTGGTTCCCTG+AGG 0.646541 8_1:+18915507 Msa1183630:CDS
GATACTTAAAACATGTGTTG+TGG 0.658184 8_1:+18915841 Msa1183630:three_prime_UTR
TTTACTCGTAAATCCACGAG+TGG 0.672996 8_1:-18915555 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAAAATATTTGAAG+TGG + chr8_1:18913763-18913782 Msa1183630:intron 10.0%
!!! TTTTTTAAAATATTTGAAGT+GGG + chr8_1:18913764-18913783 Msa1183630:intron 10.0%
!!! CCATTATTATTTTAATTTAT+CGG + chr8_1:18915807-18915826 Msa1183630:three_prime_UTR 10.0%
!!! AAAAAAAACGAAATTTTGAT+AGG - chr8_1:18913745-18913764 None:intergenic 15.0%
!!! ATTCTGGATTTTAAAATATT+TGG + chr8_1:18914531-18914550 Msa1183630:intron 15.0%
!!! CAGTTAAAATTTAAAACAAA+TGG - chr8_1:18914946-18914965 None:intergenic 15.0%
!!! TTGTTTTGAAAAAAATATCA+AGG - chr8_1:18915080-18915099 None:intergenic 15.0%
!! CCGATAAATTAAAATAATAA+TGG - chr8_1:18915810-18915829 None:intergenic 15.0%
!! CTTAATAAATCATCCTTATT+TGG + chr8_1:18913692-18913711 Msa1183630:intron 20.0%
!! CAGATAATAATAACCAAATA+AGG - chr8_1:18913708-18913727 None:intergenic 20.0%
!! TTTAGCTAAATTTATTTGTG+TGG + chr8_1:18913887-18913906 Msa1183630:intron 20.0%
!! TCATGTTATGAATTAGTAAA+TGG - chr8_1:18914246-18914265 None:intergenic 20.0%
!!! TTTGATACTTCTAAAATTTC+AGG + chr8_1:18914505-18914524 Msa1183630:intron 20.0%
!! GAAATTGAAATTATTTGTGA+TGG + chr8_1:18914607-18914626 Msa1183630:intron 20.0%
!!! TTAATCTGAATTTGAAGTTT+TGG + chr8_1:18915716-18915735 Msa1183630:three_prime_UTR 20.0%
!! CAAAATAAAAAATGAACGAT+AGG - chr8_1:18915751-18915770 None:intergenic 20.0%
! GAATTGTGAATCAAAAAAAG+AGG - chr8_1:18913620-18913639 None:intergenic 25.0%
! ACTAAAAGTTAGTATGCTAA+GGG - chr8_1:18914043-18914062 None:intergenic 25.0%
! AACTAAAAGTTAGTATGCTA+AGG - chr8_1:18914044-18914063 None:intergenic 25.0%
! GTTTGAATTTCAAAACAATC+AGG - chr8_1:18914434-18914453 None:intergenic 25.0%
!!! TGTTTTGAAATTCAAACTTG+AGG + chr8_1:18914437-18914456 Msa1183630:intron 25.0%
! TTGAAAATCAACATTTGGAA+AGG - chr8_1:18914467-18914486 None:intergenic 25.0%
! TACACTTGAAAATCAACATT+TGG - chr8_1:18914472-18914491 None:intergenic 25.0%
!!! AGGTTTGAAATTTTTGAATG+TGG + chr8_1:18914655-18914674 Msa1183630:intron 25.0%
! TAAAATCAAGTCTTAAGAAC+AGG - chr8_1:18915038-18915057 None:intergenic 25.0%
!!! GTTTTGATTGACTTAAAACA+TGG - chr8_1:18915113-18915132 None:intergenic 25.0%
! ATAAAACTTAGATAAGAGCT+TGG + chr8_1:18915173-18915192 Msa1183630:intron 25.0%
!! CAAGTTTTCAAATCAAACAA+TGG - chr8_1:18915315-18915334 None:intergenic 25.0%
!!! CTTGATTTTAAGTTACAGAA+TGG + chr8_1:18915411-18915430 Msa1183630:intron 25.0%
!!! ATTTTAATCACTATTGATGC+AGG + chr8_1:18915440-18915459 Msa1183630:intron 25.0%
! ACTAGACTTAACATCAAAAA+AGG - chr8_1:18915651-18915670 None:intergenic 25.0%
!!! ATTTTAATTTATCGGTTGTG+AGG + chr8_1:18915815-18915834 Msa1183630:three_prime_UTR 25.0%
!!! GGTGTTTTGTTGTATAATTT+TGG + chr8_1:18915862-18915881 Msa1183630:three_prime_UTR 25.0%
!!! GTTTTGGGAAAGTGAAATTA+GGG + chr8_1:18913401-18913420 Msa1183630:five_prime_UTR 30.0%
ATGCTTGACTGTTTAGAAAA+TGG + chr8_1:18913824-18913843 Msa1183630:intron 30.0%
TGCTTGACTGTTTAGAAAAT+GGG + chr8_1:18913825-18913844 Msa1183630:intron 30.0%
!! TAGAAAATGGGTTGATTTGT+TGG + chr8_1:18913837-18913856 Msa1183630:intron 30.0%
GACTGAAAAATTTGTGTTTC+AGG + chr8_1:18913965-18913984 Msa1183630:intron 30.0%
! GAAAAATTTGTGTTTCAGGT+TGG + chr8_1:18913969-18913988 Msa1183630:intron 30.0%
CTAACAATCACTCACTTATT+GGG - chr8_1:18914348-18914367 None:intergenic 30.0%
TCTAACAATCACTCACTTAT+TGG - chr8_1:18914349-18914368 None:intergenic 30.0%
! CTAAAATTTCAGGTCAATTC+TGG + chr8_1:18914515-18914534 Msa1183630:intron 30.0%
! TGATTGACTTAAAACATGGA+AGG - chr8_1:18915109-18915128 None:intergenic 30.0%
AAGTATCATTGTTAGACATG+AGG - chr8_1:18915135-18915154 None:intergenic 30.0%
TATCCTTACCAATAGCTAAT+AGG + chr8_1:18915196-18915215 Msa1183630:intron 30.0%
AATAAGCACCTATTAGCTAT+TGG - chr8_1:18915207-18915226 None:intergenic 30.0%
AATAGATGATGTTTGATGCT+GGG - chr8_1:18915258-18915277 None:intergenic 30.0%
AAATAGATGATGTTTGATGC+TGG - chr8_1:18915259-18915278 None:intergenic 30.0%
AGCATCAAACATCATCTATT+TGG + chr8_1:18915258-18915277 Msa1183630:intron 30.0%
!! GAGCACTTTTGATTAGTTAA+GGG - chr8_1:18915373-18915392 None:intergenic 30.0%
!! AGAGCACTTTTGATTAGTTA+AGG - chr8_1:18915374-18915393 None:intergenic 30.0%
!!! CTGAATTTGAAGTTTTGGTT+TGG + chr8_1:18915721-18915740 Msa1183630:three_prime_UTR 30.0%
GATACTTAAAACATGTGTTG+TGG + chr8_1:18915841-18915860 Msa1183630:three_prime_UTR 30.0%
!! TGTTGTATAATTTTGGTACG+TGG + chr8_1:18915869-18915888 Msa1183630:three_prime_UTR 30.0%
!!! TACGTGGATTTTGAAATTCA+AGG + chr8_1:18915885-18915904 Msa1183630:three_prime_UTR 30.0%
!!! CGTTTTGGGAAAGTGAAATT+AGG + chr8_1:18913400-18913419 Msa1183630:five_prime_UTR 35.0%
GGAAAGTGAAATTAGGGTTA+AGG + chr8_1:18913407-18913426 Msa1183630:five_prime_UTR 35.0%
! TCTCTGTTTGGTATTGAGTT+CGG - chr8_1:18913516-18913535 None:intergenic 35.0%
ACCAAACAGAGAAATCAAGA+TGG + chr8_1:18913524-18913543 Msa1183630:exon 35.0%
! AAAAAAGAGGTGTGAGAGAA+GGG - chr8_1:18913607-18913626 None:intergenic 35.0%
! AAAAAAAGAGGTGTGAGAGA+AGG - chr8_1:18913608-18913627 None:intergenic 35.0%
CTTGTTTGATGCTGAAAGTT+TGG + chr8_1:18913934-18913953 Msa1183630:intron 35.0%
AAGAAGGGTTTCTACTTGAA+AGG + chr8_1:18914014-18914033 Msa1183630:CDS 35.0%
TGATCAGAGAACAAAACAAC+AGG - chr8_1:18914085-18914104 None:intergenic 35.0%
TGAGATCCATTTCCAAAGTT+TGG + chr8_1:18914307-18914326 Msa1183630:intron 35.0%
GAGATCCATTTCCAAAGTTT+GGG + chr8_1:18914308-18914327 Msa1183630:intron 35.0%
! ACATATCAATTGAGAAGTGC+TGG - chr8_1:18914390-18914409 None:intergenic 35.0%
ACAACTGGCAGAAAGATTAT+TGG + chr8_1:18914800-18914819 Msa1183630:CDS 35.0%
CGTCTAGTATAGTGAATTGT+AGG + chr8_1:18915004-18915023 Msa1183630:intron 35.0%
ATATCAAGGTGACTTACATG+AGG - chr8_1:18915066-18915085 None:intergenic 35.0%
GTAAATTCACGATCAACTTC+AGG - chr8_1:18915589-18915608 None:intergenic 35.0%
TCCAGCAAAGGATCAAAATA+GGG - chr8_1:18915611-18915630 None:intergenic 35.0%
TTCCAGCAAAGGATCAAAAT+AGG - chr8_1:18915612-18915631 None:intergenic 35.0%
TTTAAGTGCAACCACATCAA+TGG - chr8_1:18915782-18915801 None:intergenic 35.0%
AGGAAAGGGGGGTTTATATA+TGG - chr8_1:18913444-18913463 None:intergenic 40.0%
GTTACTGTTGTTGCTGTGTT+GGG - chr8_1:18913494-18913513 None:intergenic 40.0%
GCCATCTTGATTTCTCTGTT+TGG - chr8_1:18913528-18913547 None:intergenic 40.0%
TTCATCATGATCAAGCCTGA+TGG + chr8_1:18913558-18913577 Msa1183630:CDS 40.0%
TATCAGCAGGTTTGAGAAGA+AGG + chr8_1:18913998-18914017 Msa1183630:CDS 40.0%
ATCAGCAGGTTTGAGAAGAA+GGG + chr8_1:18913999-18914018 Msa1183630:CDS 40.0%
! TGGATCCCAAACTTTGGAAA+TGG - chr8_1:18914316-18914335 None:intergenic 40.0%
TGGTGAATGATTCTTGAGAC+TGG + chr8_1:18914627-18914646 Msa1183630:intron 40.0%
TTGGTAGAGTACATTGTCTC+TGG + chr8_1:18914737-18914756 Msa1183630:CDS 40.0%
! TTGTTGCCATGATTTGGGAA+GGG + chr8_1:18914765-18914784 Msa1183630:CDS 40.0%
ATTCTTCCCTTCCCAAATCA+TGG - chr8_1:18914774-18914793 None:intergenic 40.0%
!! GTGACTTTGCTGTTGAGATT+GGG + chr8_1:18914864-18914883 Msa1183630:CDS 40.0%
AAGGTGACTTACATGAGGAA+TGG - chr8_1:18915061-18915080 None:intergenic 40.0%
GCACCTATTAGCTATTGGTA+AGG - chr8_1:18915202-18915221 None:intergenic 40.0%
ATTAGTTAAGGGACAGAGCT+TGG - chr8_1:18915362-18915381 None:intergenic 40.0%
TGATGCAGGAACGTTATTCA+TGG + chr8_1:18915454-18915473 Msa1183630:intron 40.0%
CCACAAACCAATTTCCTTGT+TGG - chr8_1:18915500-18915519 None:intergenic 40.0%
!! CCAACAAGGAAATTGGTTTG+TGG + chr8_1:18915497-18915516 Msa1183630:CDS 40.0%
TTTACTCGTAAATCCACGAG+TGG - chr8_1:18915558-18915577 None:intergenic 40.0%
!!! ACCCTATTTTGATCCTTTGC+TGG + chr8_1:18915607-18915626 Msa1183630:three_prime_UTR 40.0%
AACACTCAAAGTTCCAGCAA+AGG - chr8_1:18915623-18915642 None:intergenic 40.0%
!! TTGCGTTTTAGCCATTGATG+TGG + chr8_1:18915768-18915787 Msa1183630:three_prime_UTR 40.0%
TTTGCATAGACAGATCCCTT+AGG + chr8_1:18915938-18915957 Msa1183630:three_prime_UTR 40.0%
GATAGTTCCAACCAACCTAA+GGG - chr8_1:18915956-18915975 None:intergenic 40.0%
TGATAGTTCCAACCAACCTA+AGG - chr8_1:18915957-18915976 None:intergenic 40.0%
GGTTACTGTTGTTGCTGTGT+TGG - chr8_1:18913495-18913514 None:intergenic 45.0%
! AAGCCTGATGGTGTTCAAAG+AGG + chr8_1:18913570-18913589 Msa1183630:CDS 45.0%
AGTCCTCTTTGAACACCATC+AGG - chr8_1:18913576-18913595 None:intergenic 45.0%
!! AGGTTGGTGAGATTATCAGC+AGG + chr8_1:18913985-18914004 Msa1183630:intron 45.0%
AACTCGTGGATCCCAAACTT+TGG - chr8_1:18914322-18914341 None:intergenic 45.0%
CACTTATTGGGTGAAACTCG+TGG - chr8_1:18914336-18914355 None:intergenic 45.0%
GATTCTTGAGACTGGACTTC+AGG + chr8_1:18914635-18914654 Msa1183630:intron 45.0%
TCTGCAAAGCCTTTCTTCAG+CGG + chr8_1:18914713-18914732 Msa1183630:CDS 45.0%
AAAGCCTTTCTTCAGCGGAT+TGG + chr8_1:18914718-18914737 Msa1183630:CDS 45.0%
TCTACCAATCCGCTGAAGAA+AGG - chr8_1:18914725-18914744 None:intergenic 45.0%
CCCAAATCATGGCAACAACA+GGG - chr8_1:18914763-18914782 None:intergenic 45.0%
CCCTGTTGTTGCCATGATTT+GGG + chr8_1:18914760-18914779 Msa1183630:CDS 45.0%
TCCCAAATCATGGCAACAAC+AGG - chr8_1:18914764-18914783 None:intergenic 45.0%
! GTTGTTGCCATGATTTGGGA+AGG + chr8_1:18914764-18914783 Msa1183630:CDS 45.0%
GGGAAGAATGTTGTGACAAC+TGG + chr8_1:18914785-18914804 Msa1183630:CDS 45.0%
AAGTCACCACGAATAGTTCC+AGG - chr8_1:18914851-18914870 None:intergenic 45.0%
!! GGTGACTTTGCTGTTGAGAT+TGG + chr8_1:18914863-18914882 Msa1183630:CDS 45.0%
!! ACTTTGCTGTTGAGATTGGG+AGG + chr8_1:18914867-18914886 Msa1183630:CDS 45.0%
!! TTCTGTTGAAAGCGCCAACA+AGG + chr8_1:18915483-18915502 Msa1183630:CDS 45.0%
!! GAAAGCGCCAACAAGGAAAT+TGG + chr8_1:18915490-18915509 Msa1183630:CDS 45.0%
!! AATTGGTTTGTGGTTCCCTG+AGG + chr8_1:18915507-18915526 Msa1183630:CDS 45.0%
!! ATTGGTTTGTGGTTCCCTGA+GGG + chr8_1:18915508-18915527 Msa1183630:CDS 45.0%
CATAGACAGATCCCTTAGGT+TGG + chr8_1:18915942-18915961 Msa1183630:three_prime_UTR 45.0%
! TGTGGTGGTGTGAGAGGAAA+GGG - chr8_1:18913458-18913477 None:intergenic 50.0%
! TTGGGAGAGAGTGTGATGTG+TGG - chr8_1:18913476-18913495 None:intergenic 50.0%
GCCCTGTTGTTGCCATGATT+TGG + chr8_1:18914759-18914778 Msa1183630:CDS 50.0%
CTCAGATTGAGCAGGGTTTG+TGG - chr8_1:18914828-18914847 None:intergenic 50.0%
TTCCAGGCTCAGATTGAGCA+GGG - chr8_1:18914835-18914854 None:intergenic 50.0%
TCTGAGCCTGGAACTATTCG+TGG + chr8_1:18914842-18914861 Msa1183630:CDS 50.0%
ACTCGTAAATCCACGAGTGG+AGG - chr8_1:18915555-18915574 None:intergenic 50.0%
!! GACAGATCCCTTAGGTTGGT+TGG + chr8_1:18915946-18915965 Msa1183630:three_prime_UTR 50.0%
! TGGTGGTGTGAGAGGAAAGG+GGG - chr8_1:18913456-18913475 None:intergenic 55.0%
! GTGGTGGTGTGAGAGGAAAG+GGG - chr8_1:18913457-18913476 None:intergenic 55.0%
! GTGTGGTGGTGTGAGAGGAA+AGG - chr8_1:18913459-18913478 None:intergenic 55.0%
! GTGATGTGTGGTGGTGTGAG+AGG - chr8_1:18913464-18913483 None:intergenic 55.0%
! GGAGAGAGTGTGATGTGTGG+TGG - chr8_1:18913473-18913492 None:intergenic 55.0%
AACCCTGCTCAATCTGAGCC+TGG + chr8_1:18914830-18914849 Msa1183630:CDS 55.0%
GTTCCAGGCTCAGATTGAGC+AGG - chr8_1:18914836-18914855 None:intergenic 55.0%
GGTGGTGTGAGAGGAAAGGG+GGG - chr8_1:18913455-18913474 None:intergenic 60.0%
! AGGCTGCTTTGCCATGCAGC+AGG - chr8_1:18915535-18915554 None:intergenic 60.0%
TCCCTGAGGGACCTGCTGCA+TGG + chr8_1:18915521-18915540 Msa1183630:CDS 65.0%
GCCATGCAGCAGGTCCCTCA+GGG - chr8_1:18915525-18915544 None:intergenic 65.0%
TGCCATGCAGCAGGTCCCTC+AGG - chr8_1:18915526-18915545 None:intergenic 65.0%
GGCAAAGCAGCCTCCACTCG+TGG + chr8_1:18915542-18915561 Msa1183630:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 18913398 18916012 18913398 ID=Msa1183630;Name=Msa1183630
chr8_1 mRNA 18913398 18916012 18913398 ID=Msa1183630-mRNA-1;Parent=Msa1183630;Name=Msa1183630-mRNA-1;_AED=0.11;_eAED=0.11;_QI=145|1|1|1|1|1|4|436|149
chr8_1 exon 18913398 18913596 18913398 ID=Msa1183630-mRNA-1:exon:7289;Parent=Msa1183630-mRNA-1
chr8_1 exon 18913987 18914035 18913987 ID=Msa1183630-mRNA-1:exon:7290;Parent=Msa1183630-mRNA-1
chr8_1 exon 18914657 18914888 18914657 ID=Msa1183630-mRNA-1:exon:7291;Parent=Msa1183630-mRNA-1
chr8_1 exon 18915462 18916012 18915462 ID=Msa1183630-mRNA-1:exon:7292;Parent=Msa1183630-mRNA-1
chr8_1 five_prime_UTR 18913398 18913542 18913398 ID=Msa1183630-mRNA-1:five_prime_utr;Parent=Msa1183630-mRNA-1
chr8_1 CDS 18913543 18913596 18913543 ID=Msa1183630-mRNA-1:cds;Parent=Msa1183630-mRNA-1
chr8_1 CDS 18913987 18914035 18913987 ID=Msa1183630-mRNA-1:cds;Parent=Msa1183630-mRNA-1
chr8_1 CDS 18914657 18914888 18914657 ID=Msa1183630-mRNA-1:cds;Parent=Msa1183630-mRNA-1
chr8_1 CDS 18915462 18915576 18915462 ID=Msa1183630-mRNA-1:cds;Parent=Msa1183630-mRNA-1
chr8_1 three_prime_UTR 18915577 18916012 18915577 ID=Msa1183630-mRNA-1:three_prime_utr;Parent=Msa1183630-mRNA-1
Gene Sequence

>Msa1183630

ATGGCTGAACAAACTTTCATCATGATCAAGCCTGATGGTGTTCAAAGAGGACTCGTTGGTGAGATTATCAGCAGGTTTGAGAAGAAGGGTTTCTACTTGAAAGGTTTGAAATTTTTGAATGTGGAGCGTGCTTTTGCTGAGAAGCACTATGCTGATTTGTCTGCAAAGCCTTTCTTCAGCGGATTGGTAGAGTACATTGTCTCTGGCCCTGTTGTTGCCATGATTTGGGAAGGGAAGAATGTTGTGACAACTGGCAGAAAGATTATTGGTGCCACAAACCCTGCTCAATCTGAGCCTGGAACTATTCGTGGTGACTTTGCTGTTGAGATTGGGAGGAACGTTATTCATGGAAGTGATTCTGTTGAAAGCGCCAACAAGGAAATTGGTTTGTGGTTCCCTGAGGGACCTGCTGCATGGCAAAGCAGCCTCCACTCGTGGATTTACGAGTAA
Protein sequence

>Msa1183630

MAEQTFIMIKPDGVQRGLVGEIISRFEKKGFYLKGLKFLNVERAFAEKHYADLSAKPFFSGLVEYIVSGPVVAMIWEGKNVVTTGRKIIGATNPAQSEPGTIRGDFAVEIGRNVIHGSDSVESANKEIGLWFPEGPAAWQSSLHSWIYE*