AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1185230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1185230 MtrunA17_Chr2g0331251 78.788 66 14 0 53 118 325 390 3.81e-29 109
Msa1185230 MtrunA17_Chr8g0357041 54.237 59 27 0 59 117 275 333 3.55e-16 73.2
Msa1185230 MtrunA17_Chr8g0390401 45.455 66 36 0 52 117 447 512 8.86e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1185230 AT2G32230.2 61.017 59 23 0 59 117 347 405 7.28e-20 84.0
Msa1185230 AT2G32230.1 61.017 59 23 0 59 117 347 405 7.28e-20 84.0

Find 20 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGGCATCATTGTCTAAAT+TGG 0.142427 8_1:-22104758 Msa1185230:CDS
ATGTATGATGTTGTTGTTTA+TGG 0.198942 8_1:-22104473 Msa1185230:CDS
CAGCTGTAAACAGGTGATTA+TGG 0.287348 8_1:-22104419 Msa1185230:three_prime_UTR
TTATAGGACTTTGTTATGAT+TGG 0.326524 8_1:-22104892 Msa1185230:intron
CGCGACACGCCTAATCTTAG+AGG 0.344102 8_1:+22104355 None:intergenic
ATCTCTTCTGTGTTACTCTC+AGG 0.382840 8_1:-22105885 Msa1185230:intron
AGCTGATTTCAGCTGTAAAC+AGG 0.418900 8_1:-22104428 Msa1185230:exon
CAGAAGTGGCTTGAGAAGAA+TGG 0.454597 8_1:-22104497 Msa1185230:CDS
TCATTCTCGCCGCCGTTCAC+AGG 0.475453 8_1:-22105560 Msa1185230:intron
AAATGATCGAAGTTTGCCTT+AGG 0.492571 8_1:+22104721 None:intergenic
GTTGTTTATGGAGCAAATGT+AGG 0.518443 8_1:-22104461 Msa1185230:CDS
TGTTTACAGCTGAAATCAGC+TGG 0.533665 8_1:+22104430 None:intergenic
CCATGGACACCTCTAAGATT+AGG 0.547202 8_1:-22104364 Msa1185230:three_prime_UTR
AAAAGAGACTGAAAACTTCG+CGG 0.550959 8_1:-22104776 Msa1185230:CDS
CCTAATCTTAGAGGTGTCCA+TGG 0.574869 8_1:+22104364 None:intergenic
CAATGCTCATGAGCTTCTCA+AGG 0.603399 8_1:+22104809 None:intergenic
CTCTAAGATTAGGCGTGTCG+CGG 0.606492 8_1:-22104354 Msa1185230:three_prime_UTR
CAAAAGCAGTTACCTGTGAA+CGG 0.629108 8_1:+22105548 None:intergenic
TCATAACAGTCTATAAACCA+TGG 0.635600 8_1:-22104381 Msa1185230:three_prime_UTR
AAGCAGTTACCTGTGAACGG+CGG 0.765283 8_1:+22105551 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAACATAAGTTTTTATAATA+TGG + chr8_1:22104882-22104901 None:intergenic 10.0%
!! GATTTATTTATGTAATTTAT+AGG - chr8_1:22105340-22105359 Msa1185230:intron 10.0%
!!! TAACATAAGTTTTTATAATA+TGG + chr8_1:22104882-22104901 None:intergenic 10.0%
!! GATTTATTTATGTAATTTAT+AGG - chr8_1:22105340-22105359 Msa1185230:intron 10.0%
!!! TACAAAATTTTGATTTGTTT+AGG - chr8_1:22104780-22104799 Msa1185230:CDS 15.0%
!! AAAAATACTAACATAAATTG+TGG + chr8_1:22104826-22104845 None:intergenic 15.0%
!!! TACAAAATTTTGATTTGTTT+AGG - chr8_1:22104780-22104799 Msa1185230:CDS 15.0%
!! AAAAATACTAACATAAATTG+TGG + chr8_1:22104826-22104845 None:intergenic 15.0%
!!! TTCAATTGTATTTGATTTCT+AGG - chr8_1:22104422-22104441 Msa1185230:three_prime_UTR 20.0%
!!! TAAGTTTTTATAATATGGTG+AGG + chr8_1:22104877-22104896 None:intergenic 20.0%
!! AACTTATGTTAGTATGAAAT+TGG - chr8_1:22104891-22104910 Msa1185230:intron 20.0%
!! GATAAATTAGTATAATCAAG+TGG - chr8_1:22105232-22105251 Msa1185230:intron 20.0%
!! ATAAATATTACTATAGGCAT+AGG - chr8_1:22105703-22105722 Msa1185230:intron 20.0%
!!! TTCAATTGTATTTGATTTCT+AGG - chr8_1:22104422-22104441 Msa1185230:three_prime_UTR 20.0%
!!! TAAGTTTTTATAATATGGTG+AGG + chr8_1:22104877-22104896 None:intergenic 20.0%
!! AACTTATGTTAGTATGAAAT+TGG - chr8_1:22104891-22104910 Msa1185230:intron 20.0%
!! GATAAATTAGTATAATCAAG+TGG - chr8_1:22105232-22105251 Msa1185230:intron 20.0%
!! ATAAATATTACTATAGGCAT+AGG - chr8_1:22105703-22105722 Msa1185230:intron 20.0%
!! TCAAACTCAATTTGAAATGT+TGG + chr8_1:22104941-22104960 None:intergenic 25.0%
! CAAACTAGTTACAATTAAAG+GGG + chr8_1:22104984-22105003 None:intergenic 25.0%
! CCAAACTAGTTACAATTAAA+GGG + chr8_1:22104985-22105004 None:intergenic 25.0%
! TCCAAACTAGTTACAATTAA+AGG + chr8_1:22104986-22105005 None:intergenic 25.0%
! TTCTAACCACATTATTTGAA+TGG + chr8_1:22105032-22105051 None:intergenic 25.0%
! AGTGGCTATAATTATACATT+TGG - chr8_1:22105250-22105269 Msa1185230:intron 25.0%
!! TTATAGGACTTTGTTATGAT+TGG - chr8_1:22105356-22105375 Msa1185230:intron 25.0%
! ACAAAAAATACATTCTGTCT+CGG + chr8_1:22105418-22105437 None:intergenic 25.0%
! TTTAATAACACAGTGAAGAT+TGG + chr8_1:22105562-22105581 None:intergenic 25.0%
!! TTAAAAGCTGAATTTTGTGA+TGG - chr8_1:22105599-22105618 Msa1185230:CDS 25.0%
! AAAGCATAGCTATTATACAA+AGG + chr8_1:22105649-22105668 None:intergenic 25.0%
!!! ATGTATGATGTTGTTGTTTA+TGG - chr8_1:22105775-22105794 Msa1185230:intron 25.0%
!! TCAAACTCAATTTGAAATGT+TGG + chr8_1:22104941-22104960 None:intergenic 25.0%
! CAAACTAGTTACAATTAAAG+GGG + chr8_1:22104984-22105003 None:intergenic 25.0%
! CCAAACTAGTTACAATTAAA+GGG + chr8_1:22104985-22105004 None:intergenic 25.0%
! TCCAAACTAGTTACAATTAA+AGG + chr8_1:22104986-22105005 None:intergenic 25.0%
! TTCTAACCACATTATTTGAA+TGG + chr8_1:22105032-22105051 None:intergenic 25.0%
! AGTGGCTATAATTATACATT+TGG - chr8_1:22105250-22105269 Msa1185230:intron 25.0%
!! TTATAGGACTTTGTTATGAT+TGG - chr8_1:22105356-22105375 Msa1185230:intron 25.0%
! ACAAAAAATACATTCTGTCT+CGG + chr8_1:22105418-22105437 None:intergenic 25.0%
! TTTAATAACACAGTGAAGAT+TGG + chr8_1:22105562-22105581 None:intergenic 25.0%
!! TTAAAAGCTGAATTTTGTGA+TGG - chr8_1:22105599-22105618 Msa1185230:CDS 25.0%
! AAAGCATAGCTATTATACAA+AGG + chr8_1:22105649-22105668 None:intergenic 25.0%
!!! ATGTATGATGTTGTTGTTTA+TGG - chr8_1:22105775-22105794 Msa1185230:intron 25.0%
TCATATGCAATCACAGTAAA+GGG + chr8_1:22104478-22104497 None:intergenic 30.0%
TTCATATGCAATCACAGTAA+AGG + chr8_1:22104479-22104498 None:intergenic 30.0%
TGTGATTGCATATGAATTCT+AGG - chr8_1:22104483-22104502 Msa1185230:CDS 30.0%
ACAGAATTAAGAAATGGTTG+AGG + chr8_1:22104531-22104550 None:intergenic 30.0%
TCAACCATTTCTTAATTCTG+TGG - chr8_1:22104530-22104549 Msa1185230:intron 30.0%
!! ATAATGGTGAGTTGATTCTT+TGG - chr8_1:22104613-22104632 Msa1185230:intron 30.0%
! CTTTGGTAATTTATCACACT+AGG - chr8_1:22104630-22104649 Msa1185230:intron 30.0%
CCCTTTAATTGTAACTAGTT+TGG - chr8_1:22104982-22105001 Msa1185230:intron 30.0%
CTCTAACCATTCAAATAATG+TGG - chr8_1:22105023-22105042 Msa1185230:intron 30.0%
TCTATATGACATAGTAATGC+TGG - chr8_1:22105091-22105110 Msa1185230:intron 30.0%
TTCAAGAAATTGTCTTCAGT+TGG - chr8_1:22105667-22105686 Msa1185230:intron 30.0%
!! ATGTTTAACTTTTGCAGAAG+TGG - chr8_1:22105737-22105756 Msa1185230:intron 30.0%
TCATAACAGTCTATAAACCA+TGG - chr8_1:22105867-22105886 Msa1185230:intron 30.0%
TCATATGCAATCACAGTAAA+GGG + chr8_1:22104478-22104497 None:intergenic 30.0%
TTCATATGCAATCACAGTAA+AGG + chr8_1:22104479-22104498 None:intergenic 30.0%
TGTGATTGCATATGAATTCT+AGG - chr8_1:22104483-22104502 Msa1185230:CDS 30.0%
ACAGAATTAAGAAATGGTTG+AGG + chr8_1:22104531-22104550 None:intergenic 30.0%
TCAACCATTTCTTAATTCTG+TGG - chr8_1:22104530-22104549 Msa1185230:intron 30.0%
!! ATAATGGTGAGTTGATTCTT+TGG - chr8_1:22104613-22104632 Msa1185230:intron 30.0%
! CTTTGGTAATTTATCACACT+AGG - chr8_1:22104630-22104649 Msa1185230:intron 30.0%
CCCTTTAATTGTAACTAGTT+TGG - chr8_1:22104982-22105001 Msa1185230:intron 30.0%
CTCTAACCATTCAAATAATG+TGG - chr8_1:22105023-22105042 Msa1185230:intron 30.0%
TCTATATGACATAGTAATGC+TGG - chr8_1:22105091-22105110 Msa1185230:intron 30.0%
TTCAAGAAATTGTCTTCAGT+TGG - chr8_1:22105667-22105686 Msa1185230:intron 30.0%
!! ATGTTTAACTTTTGCAGAAG+TGG - chr8_1:22105737-22105756 Msa1185230:intron 30.0%
TCATAACAGTCTATAAACCA+TGG - chr8_1:22105867-22105886 Msa1185230:intron 30.0%
CATATGCAATCACAGTAAAG+GGG + chr8_1:22104477-22104496 None:intergenic 35.0%
CATTTCTTAATTCTGTGGCT+GGG - chr8_1:22104535-22104554 Msa1185230:intron 35.0%
ATTTCTTAATTCTGTGGCTG+GGG - chr8_1:22104536-22104555 Msa1185230:intron 35.0%
AGTCTGTTGCTGAAACATAA+TGG - chr8_1:22104597-22104616 Msa1185230:intron 35.0%
AGAGTCATATGCTTAAGATG+TGG - chr8_1:22105061-22105080 Msa1185230:intron 35.0%
TTGTAAACTAACCCATGCAA+AGG + chr8_1:22105152-22105171 None:intergenic 35.0%
ATCCATATGCATCACACATA+AGG + chr8_1:22105309-22105328 None:intergenic 35.0%
TGCATATGGATTTCAAACTG+TGG - chr8_1:22105318-22105337 Msa1185230:intron 35.0%
AAAAGAGACTGAAAACTTCG+CGG - chr8_1:22105472-22105491 Msa1185230:intron 35.0%
! AGTTTGCCTTAGGATGTTTT+TGG + chr8_1:22105520-22105539 None:intergenic 35.0%
AAATGATCGAAGTTTGCCTT+AGG + chr8_1:22105530-22105549 None:intergenic 35.0%
GTTGTTTATGGAGCAAATGT+AGG - chr8_1:22105787-22105806 Msa1185230:intron 35.0%
CATATGCAATCACAGTAAAG+GGG + chr8_1:22104477-22104496 None:intergenic 35.0%
CATTTCTTAATTCTGTGGCT+GGG - chr8_1:22104535-22104554 Msa1185230:intron 35.0%
ATTTCTTAATTCTGTGGCTG+GGG - chr8_1:22104536-22104555 Msa1185230:intron 35.0%
AGTCTGTTGCTGAAACATAA+TGG - chr8_1:22104597-22104616 Msa1185230:intron 35.0%
AGAGTCATATGCTTAAGATG+TGG - chr8_1:22105061-22105080 Msa1185230:intron 35.0%
TTGTAAACTAACCCATGCAA+AGG + chr8_1:22105152-22105171 None:intergenic 35.0%
ATCCATATGCATCACACATA+AGG + chr8_1:22105309-22105328 None:intergenic 35.0%
TGCATATGGATTTCAAACTG+TGG - chr8_1:22105318-22105337 Msa1185230:intron 35.0%
AAAAGAGACTGAAAACTTCG+CGG - chr8_1:22105472-22105491 Msa1185230:intron 35.0%
! AGTTTGCCTTAGGATGTTTT+TGG + chr8_1:22105520-22105539 None:intergenic 35.0%
AAATGATCGAAGTTTGCCTT+AGG + chr8_1:22105530-22105549 None:intergenic 35.0%
GTTGTTTATGGAGCAAATGT+AGG - chr8_1:22105787-22105806 Msa1185230:intron 35.0%
! ATCTCTTCTGTGTTACTCTC+AGG - chr8_1:22104363-22104382 Msa1185230:three_prime_UTR 40.0%
CACAGTAAAGGGGAATTCAA+CGG + chr8_1:22104467-22104486 None:intergenic 40.0%
CCAGCCACAGAATTAAGAAA+TGG + chr8_1:22104537-22104556 None:intergenic 40.0%
CCATTTCTTAATTCTGTGGC+TGG - chr8_1:22104534-22104553 Msa1185230:intron 40.0%
CAAAAGCAGTTACCTGTGAA+CGG + chr8_1:22104703-22104722 None:intergenic 40.0%
AGTCATCTTGAACCTTTGCA+TGG - chr8_1:22105137-22105156 Msa1185230:intron 40.0%
GTCATCTTGAACCTTTGCAT+GGG - chr8_1:22105138-22105157 Msa1185230:intron 40.0%
TGCCTTATGTGTGATGCATA+TGG - chr8_1:22105304-22105323 Msa1185230:intron 40.0%
! GCGAAGTTTTCAGTCTCTTT+TGG + chr8_1:22105473-22105492 None:intergenic 40.0%
! GGCAAACTTCGATCATTTTC+AGG - chr8_1:22105532-22105551 Msa1185230:intron 40.0%
TGTTTACAGCTGAAATCAGC+TGG + chr8_1:22105821-22105840 None:intergenic 40.0%
! AGCTGATTTCAGCTGTAAAC+AGG - chr8_1:22105820-22105839 Msa1185230:intron 40.0%
CAGCTGTAAACAGGTGATTA+TGG - chr8_1:22105829-22105848 Msa1185230:intron 40.0%
! ATCTCTTCTGTGTTACTCTC+AGG - chr8_1:22104363-22104382 Msa1185230:three_prime_UTR 40.0%
CACAGTAAAGGGGAATTCAA+CGG + chr8_1:22104467-22104486 None:intergenic 40.0%
CCAGCCACAGAATTAAGAAA+TGG + chr8_1:22104537-22104556 None:intergenic 40.0%
CCATTTCTTAATTCTGTGGC+TGG - chr8_1:22104534-22104553 Msa1185230:intron 40.0%
CAAAAGCAGTTACCTGTGAA+CGG + chr8_1:22104703-22104722 None:intergenic 40.0%
AGTCATCTTGAACCTTTGCA+TGG - chr8_1:22105137-22105156 Msa1185230:intron 40.0%
GTCATCTTGAACCTTTGCAT+GGG - chr8_1:22105138-22105157 Msa1185230:intron 40.0%
TGCCTTATGTGTGATGCATA+TGG - chr8_1:22105304-22105323 Msa1185230:intron 40.0%
! GCGAAGTTTTCAGTCTCTTT+TGG + chr8_1:22105473-22105492 None:intergenic 40.0%
! GGCAAACTTCGATCATTTTC+AGG - chr8_1:22105532-22105551 Msa1185230:intron 40.0%
TGTTTACAGCTGAAATCAGC+TGG + chr8_1:22105821-22105840 None:intergenic 40.0%
! AGCTGATTTCAGCTGTAAAC+AGG - chr8_1:22105820-22105839 Msa1185230:intron 40.0%
CAGCTGTAAACAGGTGATTA+TGG - chr8_1:22105829-22105848 Msa1185230:intron 40.0%
CAATGCTCATGAGCTTCTCA+AGG + chr8_1:22105442-22105461 None:intergenic 45.0%
CGCGGCATCATTGTCTAAAT+TGG - chr8_1:22105490-22105509 Msa1185230:intron 45.0%
GGCTTGCCAAAAACATCCTA+AGG - chr8_1:22105511-22105530 Msa1185230:intron 45.0%
CAGAAGTGGCTTGAGAAGAA+TGG - chr8_1:22105751-22105770 Msa1185230:intron 45.0%
!! CCTAATCTTAGAGGTGTCCA+TGG + chr8_1:22105887-22105906 None:intergenic 45.0%
!! CCATGGACACCTCTAAGATT+AGG - chr8_1:22105884-22105903 Msa1185230:intron 45.0%
CAATGCTCATGAGCTTCTCA+AGG + chr8_1:22105442-22105461 None:intergenic 45.0%
CGCGGCATCATTGTCTAAAT+TGG - chr8_1:22105490-22105509 Msa1185230:intron 45.0%
GGCTTGCCAAAAACATCCTA+AGG - chr8_1:22105511-22105530 Msa1185230:intron 45.0%
CAGAAGTGGCTTGAGAAGAA+TGG - chr8_1:22105751-22105770 Msa1185230:intron 45.0%
!! CCTAATCTTAGAGGTGTCCA+TGG + chr8_1:22105887-22105906 None:intergenic 45.0%
!! CCATGGACACCTCTAAGATT+AGG - chr8_1:22105884-22105903 Msa1185230:intron 45.0%
!! TAATATATAAATATTACTAT+AGG - chr8_1:22105697-22105716 Msa1185230:intron 5.0%
!! TAATATATAAATATTACTAT+AGG - chr8_1:22105697-22105716 Msa1185230:intron 5.0%
AAGCAGTTACCTGTGAACGG+CGG + chr8_1:22104700-22104719 None:intergenic 50.0%
!! CTCTAAGATTAGGCGTGTCG+CGG - chr8_1:22105894-22105913 Msa1185230:CDS 50.0%
AAGCAGTTACCTGTGAACGG+CGG + chr8_1:22104700-22104719 None:intergenic 50.0%
!! CTCTAAGATTAGGCGTGTCG+CGG - chr8_1:22105894-22105913 Msa1185230:CDS 50.0%
CGCGACACGCCTAATCTTAG+AGG + chr8_1:22105896-22105915 None:intergenic 55.0%
CGCGACACGCCTAATCTTAG+AGG + chr8_1:22105896-22105915 None:intergenic 55.0%
TCATTCTCGCCGCCGTTCAC+AGG - chr8_1:22104688-22104707 Msa1185230:intron 60.0%
TCATTCTCGCCGCCGTTCAC+AGG - chr8_1:22104688-22104707 Msa1185230:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 22104344 22105926 22104344 ID=Msa1185230;Name=Msa1185230
chr8_1 mRNA 22104344 22105926 22104344 ID=Msa1185230-mRNA-1;Parent=Msa1185230;Name=Msa1185230-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0.33|0.25|0.5|1|0.75|4|101|120
chr8_1 exon 22104344 22104516 22104344 ID=Msa1185230-mRNA-1:exon:8210;Parent=Msa1185230-mRNA-1
chr8_1 exon 22104717 22104908 22104717 ID=Msa1185230-mRNA-1:exon:8209;Parent=Msa1185230-mRNA-1
chr8_1 exon 22105561 22105618 22105561 ID=Msa1185230-mRNA-1:exon:8208;Parent=Msa1185230-mRNA-1
chr8_1 exon 22105886 22105926 22105886 ID=Msa1185230-mRNA-1:exon:8207;Parent=Msa1185230-mRNA-1
chr8_1 CDS 22105886 22105926 22105886 ID=Msa1185230-mRNA-1:cds;Parent=Msa1185230-mRNA-1
chr8_1 CDS 22105561 22105618 22105561 ID=Msa1185230-mRNA-1:cds;Parent=Msa1185230-mRNA-1
chr8_1 CDS 22104717 22104908 22104717 ID=Msa1185230-mRNA-1:cds;Parent=Msa1185230-mRNA-1
chr8_1 CDS 22104445 22104516 22104445 ID=Msa1185230-mRNA-1:cds;Parent=Msa1185230-mRNA-1
chr8_1 three_prime_UTR 22104344 22104444 22104344 ID=Msa1185230-mRNA-1:three_prime_utr;Parent=Msa1185230-mRNA-1
Gene Sequence

>Msa1185230

ATGATCAACACTATCTTTGATCTCTTCTGTGTTACTCTCAGGTGTTCACAAGTAACTGCTCTCAATCATTCTTATCATCATTCTCGCCGCCGTTCACAGGACTTTGTTATGATTGGTTTCAATAGAATTTCATTTCAGTTCATGACATATTGCCGAGACAGAATGTATTTTTTGTGCCTTGAGAAGCTCATGAGCATTGATATTGATCCAAAAGAGACTGAAAACTTCGCGGCATCATTGTCTAAATTGGCTTGCCAAAAACATCCTAAGGCAAACTTCGATCATTTTCAGAAGTGGCTTGAGAAGAATGGCATGTATGATGTTGTTGTTTATGGAGCAAATGTAGGACTTGCAACCAGCTGA

Protein sequence

>Msa1185230

MINTIFDLFCVTLRCSQVTALNHSYHHSRRRSQDFVMIGFNRISFQFMTYCRDRMYFLCLEKLMSIDIDPKETENFAASLSKLACQKHPKANFDHFQKWLEKNGMYDVVVYGANVGLATS*