AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1185250


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1185250 MtrunA17_Chr2g0331251 78.788 66 14 0 53 118 325 390 3.81e-29 109
Msa1185250 MtrunA17_Chr8g0357041 54.237 59 27 0 59 117 275 333 3.55e-16 73.2
Msa1185250 MtrunA17_Chr8g0390401 45.455 66 36 0 52 117 447 512 8.86e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1185250 AT2G32230.2 61.017 59 23 0 59 117 347 405 7.28e-20 84.0
Msa1185250 AT2G32230.1 61.017 59 23 0 59 117 347 405 7.28e-20 84.0

Find 20 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGGCATCATTGTCTAAAT+TGG 0.142427 8_1:-22192181 Msa1185250:CDS
ATGTATGATGTTGTTGTTTA+TGG 0.198942 8_1:-22191896 Msa1185250:CDS
CAGCTGTAAACAGGTGATTA+TGG 0.287348 8_1:-22191842 Msa1185250:three_prime_UTR
TTATAGGACTTTGTTATGAT+TGG 0.326524 8_1:-22192315 Msa1185250:intron
CGCGACACGCCTAATCTTAG+AGG 0.344102 8_1:+22191778 None:intergenic
ATCTCTTCTGTGTTACTCTC+AGG 0.382840 8_1:-22193308 Msa1185250:intron
AGCTGATTTCAGCTGTAAAC+AGG 0.418900 8_1:-22191851 Msa1185250:exon
CAGAAGTGGCTTGAGAAGAA+TGG 0.454597 8_1:-22191920 Msa1185250:CDS
TCATTCTCGCCGCCGTTCAC+AGG 0.475453 8_1:-22192983 Msa1185250:intron
AAATGATCGAAGTTTGCCTT+AGG 0.492571 8_1:+22192144 None:intergenic
GTTGTTTATGGAGCAAATGT+AGG 0.518443 8_1:-22191884 Msa1185250:CDS
TGTTTACAGCTGAAATCAGC+TGG 0.533665 8_1:+22191853 None:intergenic
CCATGGACACCTCTAAGATT+AGG 0.547202 8_1:-22191787 Msa1185250:three_prime_UTR
AAAAGAGACTGAAAACTTCG+CGG 0.550959 8_1:-22192199 Msa1185250:CDS
CCTAATCTTAGAGGTGTCCA+TGG 0.574869 8_1:+22191787 None:intergenic
CAATGCTCATGAGCTTCTCA+AGG 0.603399 8_1:+22192232 None:intergenic
CTCTAAGATTAGGCGTGTCG+CGG 0.606492 8_1:-22191777 Msa1185250:three_prime_UTR
CAAAAGCAGTTACCTGTGAA+CGG 0.629108 8_1:+22192971 None:intergenic
TCATAACAGTCTATAAACCA+TGG 0.635600 8_1:-22191804 Msa1185250:three_prime_UTR
AAGCAGTTACCTGTGAACGG+CGG 0.765283 8_1:+22192974 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAACATAAGTTTTTATAATA+TGG + chr8_1:22192305-22192324 None:intergenic 10.0%
!! GATTTATTTATGTAATTTAT+AGG - chr8_1:22192763-22192782 Msa1185250:intron 10.0%
!!! TAACATAAGTTTTTATAATA+TGG + chr8_1:22192305-22192324 None:intergenic 10.0%
!! GATTTATTTATGTAATTTAT+AGG - chr8_1:22192763-22192782 Msa1185250:intron 10.0%
!!! TACAAAATTTTGATTTGTTT+AGG - chr8_1:22192203-22192222 Msa1185250:CDS 15.0%
!! AAAAATACTAACATAAATTG+TGG + chr8_1:22192249-22192268 None:intergenic 15.0%
!!! TACAAAATTTTGATTTGTTT+AGG - chr8_1:22192203-22192222 Msa1185250:CDS 15.0%
!! AAAAATACTAACATAAATTG+TGG + chr8_1:22192249-22192268 None:intergenic 15.0%
!!! TTCAATTGTATTTGATTTCT+AGG - chr8_1:22191845-22191864 Msa1185250:three_prime_UTR 20.0%
!!! TAAGTTTTTATAATATGGTG+AGG + chr8_1:22192300-22192319 None:intergenic 20.0%
!! AACTTATGTTAGTATGAAAT+TGG - chr8_1:22192314-22192333 Msa1185250:intron 20.0%
!! GATAAATTAGTATAATCAAG+TGG - chr8_1:22192655-22192674 Msa1185250:intron 20.0%
!! ATAAATATTACTATAGGCAT+AGG - chr8_1:22193126-22193145 Msa1185250:intron 20.0%
!!! TTCAATTGTATTTGATTTCT+AGG - chr8_1:22191845-22191864 Msa1185250:three_prime_UTR 20.0%
!!! TAAGTTTTTATAATATGGTG+AGG + chr8_1:22192300-22192319 None:intergenic 20.0%
!! AACTTATGTTAGTATGAAAT+TGG - chr8_1:22192314-22192333 Msa1185250:intron 20.0%
!! GATAAATTAGTATAATCAAG+TGG - chr8_1:22192655-22192674 Msa1185250:intron 20.0%
!! ATAAATATTACTATAGGCAT+AGG - chr8_1:22193126-22193145 Msa1185250:intron 20.0%
!! TCAAACTCAATTTGAAATGT+TGG + chr8_1:22192364-22192383 None:intergenic 25.0%
! CAAACTAGTTACAATTAAAG+GGG + chr8_1:22192407-22192426 None:intergenic 25.0%
! CCAAACTAGTTACAATTAAA+GGG + chr8_1:22192408-22192427 None:intergenic 25.0%
! TCCAAACTAGTTACAATTAA+AGG + chr8_1:22192409-22192428 None:intergenic 25.0%
! TTCTAACCACATTATTTGAA+TGG + chr8_1:22192455-22192474 None:intergenic 25.0%
! AGTGGCTATAATTATACATT+TGG - chr8_1:22192673-22192692 Msa1185250:intron 25.0%
!! TTATAGGACTTTGTTATGAT+TGG - chr8_1:22192779-22192798 Msa1185250:intron 25.0%
! ACAAAAAATACATTCTGTCT+CGG + chr8_1:22192841-22192860 None:intergenic 25.0%
! TTTAATAACACAGTGAAGAT+TGG + chr8_1:22192985-22193004 None:intergenic 25.0%
!! TTAAAAGCTGAATTTTGTGA+TGG - chr8_1:22193022-22193041 Msa1185250:CDS 25.0%
! AAAGCATAGCTATTATACAA+AGG + chr8_1:22193072-22193091 None:intergenic 25.0%
!!! ATGTATGATGTTGTTGTTTA+TGG - chr8_1:22193198-22193217 Msa1185250:intron 25.0%
!! TCAAACTCAATTTGAAATGT+TGG + chr8_1:22192364-22192383 None:intergenic 25.0%
! CAAACTAGTTACAATTAAAG+GGG + chr8_1:22192407-22192426 None:intergenic 25.0%
! CCAAACTAGTTACAATTAAA+GGG + chr8_1:22192408-22192427 None:intergenic 25.0%
! TCCAAACTAGTTACAATTAA+AGG + chr8_1:22192409-22192428 None:intergenic 25.0%
! TTCTAACCACATTATTTGAA+TGG + chr8_1:22192455-22192474 None:intergenic 25.0%
! AGTGGCTATAATTATACATT+TGG - chr8_1:22192673-22192692 Msa1185250:intron 25.0%
!! TTATAGGACTTTGTTATGAT+TGG - chr8_1:22192779-22192798 Msa1185250:intron 25.0%
! ACAAAAAATACATTCTGTCT+CGG + chr8_1:22192841-22192860 None:intergenic 25.0%
! TTTAATAACACAGTGAAGAT+TGG + chr8_1:22192985-22193004 None:intergenic 25.0%
!! TTAAAAGCTGAATTTTGTGA+TGG - chr8_1:22193022-22193041 Msa1185250:CDS 25.0%
! AAAGCATAGCTATTATACAA+AGG + chr8_1:22193072-22193091 None:intergenic 25.0%
!!! ATGTATGATGTTGTTGTTTA+TGG - chr8_1:22193198-22193217 Msa1185250:intron 25.0%
TCATATGCAATCACAGTAAA+GGG + chr8_1:22191901-22191920 None:intergenic 30.0%
TTCATATGCAATCACAGTAA+AGG + chr8_1:22191902-22191921 None:intergenic 30.0%
TGTGATTGCATATGAATTCT+AGG - chr8_1:22191906-22191925 Msa1185250:CDS 30.0%
ACAGAATTAAGAAATGGTTG+AGG + chr8_1:22191954-22191973 None:intergenic 30.0%
TCAACCATTTCTTAATTCTG+TGG - chr8_1:22191953-22191972 Msa1185250:intron 30.0%
!! ATAATGGTGAGTTGATTCTT+TGG - chr8_1:22192036-22192055 Msa1185250:intron 30.0%
! CTTTGGTAATTTATCACACT+AGG - chr8_1:22192053-22192072 Msa1185250:intron 30.0%
CCCTTTAATTGTAACTAGTT+TGG - chr8_1:22192405-22192424 Msa1185250:intron 30.0%
CTCTAACCATTCAAATAATG+TGG - chr8_1:22192446-22192465 Msa1185250:intron 30.0%
TCTATATGACATAGTAATGC+TGG - chr8_1:22192514-22192533 Msa1185250:intron 30.0%
TTCAAGAAATTGTCTTCAGT+TGG - chr8_1:22193090-22193109 Msa1185250:intron 30.0%
!! ATGTTTAACTTTTGCAGAAG+TGG - chr8_1:22193160-22193179 Msa1185250:intron 30.0%
TCATAACAGTCTATAAACCA+TGG - chr8_1:22193290-22193309 Msa1185250:intron 30.0%
TCATATGCAATCACAGTAAA+GGG + chr8_1:22191901-22191920 None:intergenic 30.0%
TTCATATGCAATCACAGTAA+AGG + chr8_1:22191902-22191921 None:intergenic 30.0%
TGTGATTGCATATGAATTCT+AGG - chr8_1:22191906-22191925 Msa1185250:CDS 30.0%
ACAGAATTAAGAAATGGTTG+AGG + chr8_1:22191954-22191973 None:intergenic 30.0%
TCAACCATTTCTTAATTCTG+TGG - chr8_1:22191953-22191972 Msa1185250:intron 30.0%
!! ATAATGGTGAGTTGATTCTT+TGG - chr8_1:22192036-22192055 Msa1185250:intron 30.0%
! CTTTGGTAATTTATCACACT+AGG - chr8_1:22192053-22192072 Msa1185250:intron 30.0%
CCCTTTAATTGTAACTAGTT+TGG - chr8_1:22192405-22192424 Msa1185250:intron 30.0%
CTCTAACCATTCAAATAATG+TGG - chr8_1:22192446-22192465 Msa1185250:intron 30.0%
TCTATATGACATAGTAATGC+TGG - chr8_1:22192514-22192533 Msa1185250:intron 30.0%
TTCAAGAAATTGTCTTCAGT+TGG - chr8_1:22193090-22193109 Msa1185250:intron 30.0%
!! ATGTTTAACTTTTGCAGAAG+TGG - chr8_1:22193160-22193179 Msa1185250:intron 30.0%
TCATAACAGTCTATAAACCA+TGG - chr8_1:22193290-22193309 Msa1185250:intron 30.0%
CATATGCAATCACAGTAAAG+GGG + chr8_1:22191900-22191919 None:intergenic 35.0%
CATTTCTTAATTCTGTGGCT+GGG - chr8_1:22191958-22191977 Msa1185250:intron 35.0%
ATTTCTTAATTCTGTGGCTG+GGG - chr8_1:22191959-22191978 Msa1185250:intron 35.0%
AGTCTGTTGCTGAAACATAA+TGG - chr8_1:22192020-22192039 Msa1185250:intron 35.0%
AGAGTCATATGCTTAAGATG+TGG - chr8_1:22192484-22192503 Msa1185250:intron 35.0%
TTGTAAACTAACCCATGCAA+AGG + chr8_1:22192575-22192594 None:intergenic 35.0%
ATCCATATGCATCACACATA+AGG + chr8_1:22192732-22192751 None:intergenic 35.0%
TGCATATGGATTTCAAACTG+TGG - chr8_1:22192741-22192760 Msa1185250:intron 35.0%
AAAAGAGACTGAAAACTTCG+CGG - chr8_1:22192895-22192914 Msa1185250:intron 35.0%
! AGTTTGCCTTAGGATGTTTT+TGG + chr8_1:22192943-22192962 None:intergenic 35.0%
AAATGATCGAAGTTTGCCTT+AGG + chr8_1:22192953-22192972 None:intergenic 35.0%
GTTGTTTATGGAGCAAATGT+AGG - chr8_1:22193210-22193229 Msa1185250:intron 35.0%
CATATGCAATCACAGTAAAG+GGG + chr8_1:22191900-22191919 None:intergenic 35.0%
CATTTCTTAATTCTGTGGCT+GGG - chr8_1:22191958-22191977 Msa1185250:intron 35.0%
ATTTCTTAATTCTGTGGCTG+GGG - chr8_1:22191959-22191978 Msa1185250:intron 35.0%
AGTCTGTTGCTGAAACATAA+TGG - chr8_1:22192020-22192039 Msa1185250:intron 35.0%
AGAGTCATATGCTTAAGATG+TGG - chr8_1:22192484-22192503 Msa1185250:intron 35.0%
TTGTAAACTAACCCATGCAA+AGG + chr8_1:22192575-22192594 None:intergenic 35.0%
ATCCATATGCATCACACATA+AGG + chr8_1:22192732-22192751 None:intergenic 35.0%
TGCATATGGATTTCAAACTG+TGG - chr8_1:22192741-22192760 Msa1185250:intron 35.0%
AAAAGAGACTGAAAACTTCG+CGG - chr8_1:22192895-22192914 Msa1185250:intron 35.0%
! AGTTTGCCTTAGGATGTTTT+TGG + chr8_1:22192943-22192962 None:intergenic 35.0%
AAATGATCGAAGTTTGCCTT+AGG + chr8_1:22192953-22192972 None:intergenic 35.0%
GTTGTTTATGGAGCAAATGT+AGG - chr8_1:22193210-22193229 Msa1185250:intron 35.0%
! ATCTCTTCTGTGTTACTCTC+AGG - chr8_1:22191786-22191805 Msa1185250:three_prime_UTR 40.0%
CACAGTAAAGGGGAATTCAA+CGG + chr8_1:22191890-22191909 None:intergenic 40.0%
CCAGCCACAGAATTAAGAAA+TGG + chr8_1:22191960-22191979 None:intergenic 40.0%
CCATTTCTTAATTCTGTGGC+TGG - chr8_1:22191957-22191976 Msa1185250:intron 40.0%
CAAAAGCAGTTACCTGTGAA+CGG + chr8_1:22192126-22192145 None:intergenic 40.0%
AGTCATCTTGAACCTTTGCA+TGG - chr8_1:22192560-22192579 Msa1185250:intron 40.0%
GTCATCTTGAACCTTTGCAT+GGG - chr8_1:22192561-22192580 Msa1185250:intron 40.0%
TGCCTTATGTGTGATGCATA+TGG - chr8_1:22192727-22192746 Msa1185250:intron 40.0%
! GCGAAGTTTTCAGTCTCTTT+TGG + chr8_1:22192896-22192915 None:intergenic 40.0%
! GGCAAACTTCGATCATTTTC+AGG - chr8_1:22192955-22192974 Msa1185250:intron 40.0%
TGTTTACAGCTGAAATCAGC+TGG + chr8_1:22193244-22193263 None:intergenic 40.0%
! AGCTGATTTCAGCTGTAAAC+AGG - chr8_1:22193243-22193262 Msa1185250:intron 40.0%
CAGCTGTAAACAGGTGATTA+TGG - chr8_1:22193252-22193271 Msa1185250:intron 40.0%
! ATCTCTTCTGTGTTACTCTC+AGG - chr8_1:22191786-22191805 Msa1185250:three_prime_UTR 40.0%
CACAGTAAAGGGGAATTCAA+CGG + chr8_1:22191890-22191909 None:intergenic 40.0%
CCAGCCACAGAATTAAGAAA+TGG + chr8_1:22191960-22191979 None:intergenic 40.0%
CCATTTCTTAATTCTGTGGC+TGG - chr8_1:22191957-22191976 Msa1185250:intron 40.0%
CAAAAGCAGTTACCTGTGAA+CGG + chr8_1:22192126-22192145 None:intergenic 40.0%
AGTCATCTTGAACCTTTGCA+TGG - chr8_1:22192560-22192579 Msa1185250:intron 40.0%
GTCATCTTGAACCTTTGCAT+GGG - chr8_1:22192561-22192580 Msa1185250:intron 40.0%
TGCCTTATGTGTGATGCATA+TGG - chr8_1:22192727-22192746 Msa1185250:intron 40.0%
! GCGAAGTTTTCAGTCTCTTT+TGG + chr8_1:22192896-22192915 None:intergenic 40.0%
! GGCAAACTTCGATCATTTTC+AGG - chr8_1:22192955-22192974 Msa1185250:intron 40.0%
TGTTTACAGCTGAAATCAGC+TGG + chr8_1:22193244-22193263 None:intergenic 40.0%
! AGCTGATTTCAGCTGTAAAC+AGG - chr8_1:22193243-22193262 Msa1185250:intron 40.0%
CAGCTGTAAACAGGTGATTA+TGG - chr8_1:22193252-22193271 Msa1185250:intron 40.0%
CAATGCTCATGAGCTTCTCA+AGG + chr8_1:22192865-22192884 None:intergenic 45.0%
CGCGGCATCATTGTCTAAAT+TGG - chr8_1:22192913-22192932 Msa1185250:intron 45.0%
GGCTTGCCAAAAACATCCTA+AGG - chr8_1:22192934-22192953 Msa1185250:intron 45.0%
CAGAAGTGGCTTGAGAAGAA+TGG - chr8_1:22193174-22193193 Msa1185250:intron 45.0%
!! CCTAATCTTAGAGGTGTCCA+TGG + chr8_1:22193310-22193329 None:intergenic 45.0%
!! CCATGGACACCTCTAAGATT+AGG - chr8_1:22193307-22193326 Msa1185250:intron 45.0%
CAATGCTCATGAGCTTCTCA+AGG + chr8_1:22192865-22192884 None:intergenic 45.0%
CGCGGCATCATTGTCTAAAT+TGG - chr8_1:22192913-22192932 Msa1185250:intron 45.0%
GGCTTGCCAAAAACATCCTA+AGG - chr8_1:22192934-22192953 Msa1185250:intron 45.0%
CAGAAGTGGCTTGAGAAGAA+TGG - chr8_1:22193174-22193193 Msa1185250:intron 45.0%
!! CCTAATCTTAGAGGTGTCCA+TGG + chr8_1:22193310-22193329 None:intergenic 45.0%
!! CCATGGACACCTCTAAGATT+AGG - chr8_1:22193307-22193326 Msa1185250:intron 45.0%
!! TAATATATAAATATTACTAT+AGG - chr8_1:22193120-22193139 Msa1185250:intron 5.0%
!! TAATATATAAATATTACTAT+AGG - chr8_1:22193120-22193139 Msa1185250:intron 5.0%
AAGCAGTTACCTGTGAACGG+CGG + chr8_1:22192123-22192142 None:intergenic 50.0%
!! CTCTAAGATTAGGCGTGTCG+CGG - chr8_1:22193317-22193336 Msa1185250:CDS 50.0%
AAGCAGTTACCTGTGAACGG+CGG + chr8_1:22192123-22192142 None:intergenic 50.0%
!! CTCTAAGATTAGGCGTGTCG+CGG - chr8_1:22193317-22193336 Msa1185250:CDS 50.0%
CGCGACACGCCTAATCTTAG+AGG + chr8_1:22193319-22193338 None:intergenic 55.0%
CGCGACACGCCTAATCTTAG+AGG + chr8_1:22193319-22193338 None:intergenic 55.0%
TCATTCTCGCCGCCGTTCAC+AGG - chr8_1:22192111-22192130 Msa1185250:intron 60.0%
TCATTCTCGCCGCCGTTCAC+AGG - chr8_1:22192111-22192130 Msa1185250:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 22191767 22193349 22191767 ID=Msa1185250;Name=Msa1185250
chr8_1 mRNA 22191767 22193349 22191767 ID=Msa1185250-mRNA-1;Parent=Msa1185250;Name=Msa1185250-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0.33|0.25|0.5|1|0.75|4|101|120
chr8_1 exon 22191767 22191939 22191767 ID=Msa1185250-mRNA-1:exon:8237;Parent=Msa1185250-mRNA-1
chr8_1 exon 22192140 22192331 22192140 ID=Msa1185250-mRNA-1:exon:8236;Parent=Msa1185250-mRNA-1
chr8_1 exon 22192984 22193041 22192984 ID=Msa1185250-mRNA-1:exon:8235;Parent=Msa1185250-mRNA-1
chr8_1 exon 22193309 22193349 22193309 ID=Msa1185250-mRNA-1:exon:8234;Parent=Msa1185250-mRNA-1
chr8_1 CDS 22193309 22193349 22193309 ID=Msa1185250-mRNA-1:cds;Parent=Msa1185250-mRNA-1
chr8_1 CDS 22192984 22193041 22192984 ID=Msa1185250-mRNA-1:cds;Parent=Msa1185250-mRNA-1
chr8_1 CDS 22192140 22192331 22192140 ID=Msa1185250-mRNA-1:cds;Parent=Msa1185250-mRNA-1
chr8_1 CDS 22191868 22191939 22191868 ID=Msa1185250-mRNA-1:cds;Parent=Msa1185250-mRNA-1
chr8_1 three_prime_UTR 22191767 22191867 22191767 ID=Msa1185250-mRNA-1:three_prime_utr;Parent=Msa1185250-mRNA-1
Gene Sequence

>Msa1185250

ATGATCAACACTATCTTTGATCTCTTCTGTGTTACTCTCAGGTGTTCACAAGTAACTGCTCTCAATCATTCTTATCATCATTCTCGCCGCCGTTCACAGGACTTTGTTATGATTGGTTTCAATAGAATTTCATTTCAGTTCATGACATATTGCCGAGACAGAATGTATTTTTTGTGCCTTGAGAAGCTCATGAGCATTGATATTGATCCAAAAGAGACTGAAAACTTCGCGGCATCATTGTCTAAATTGGCTTGCCAAAAACATCCTAAGGCAAACTTCGATCATTTTCAGAAGTGGCTTGAGAAGAATGGCATGTATGATGTTGTTGTTTATGGAGCAAATGTAGGACTTGCAACCAGCTGA

Protein sequence

>Msa1185250

MINTIFDLFCVTLRCSQVTALNHSYHHSRRRSQDFVMIGFNRISFQFMTYCRDRMYFLCLEKLMSIDIDPKETENFAASLSKLACQKHPKANFDHFQKWLEKNGMYDVVVYGANVGLATS*