AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1185240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1185240 MtrunA17_Chr2g0331251 78.788 66 14 0 53 118 325 390 3.81e-29 109
Msa1185240 MtrunA17_Chr8g0357041 54.237 59 27 0 59 117 275 333 3.55e-16 73.2
Msa1185240 MtrunA17_Chr8g0390401 45.455 66 36 0 52 117 447 512 8.86e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1185240 AT2G32230.2 61.017 59 23 0 59 117 347 405 7.28e-20 84.0
Msa1185240 AT2G32230.1 61.017 59 23 0 59 117 347 405 7.28e-20 84.0

Find 20 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGGCATCATTGTCTAAAT+TGG 0.142427 8_1:-22133009 Msa1185240:CDS
ATGTATGATGTTGTTGTTTA+TGG 0.198942 8_1:-22132724 Msa1185240:CDS
CAGCTGTAAACAGGTGATTA+TGG 0.287348 8_1:-22132670 Msa1185240:three_prime_UTR
TTATAGGACTTTGTTATGAT+TGG 0.326524 8_1:-22133143 Msa1185240:intron
CGCGACACGCCTATTCTTAG+AGG 0.354213 8_1:+22132606 None:intergenic
ATCTCTTCTGTGTTACTCTC+AGG 0.382840 8_1:-22134136 Msa1185240:intron
AGCTGATTTCAGCTGTAAAC+AGG 0.418900 8_1:-22132679 Msa1185240:exon
CAGAAGTGGCTTGAGAAGAA+TGG 0.454597 8_1:-22132748 Msa1185240:CDS
CCATGGACACCTCTAAGAAT+AGG 0.459016 8_1:-22132615 Msa1185240:three_prime_UTR
TCATTCTCGCCGCCGTTCAC+AGG 0.475453 8_1:-22133811 Msa1185240:intron
AAATGATCGAAGTTTGCCTT+AGG 0.492571 8_1:+22132972 None:intergenic
GTTGTTTATGGAGCAAATGT+AGG 0.518443 8_1:-22132712 Msa1185240:CDS
CCTATTCTTAGAGGTGTCCA+TGG 0.527953 8_1:+22132615 None:intergenic
TGTTTACAGCTGAAATCAGC+TGG 0.533665 8_1:+22132681 None:intergenic
AAAAGAGACTGAAAACTTCG+CGG 0.550959 8_1:-22133027 Msa1185240:CDS
CAATGCTCATGAGCTTCTCA+AGG 0.603399 8_1:+22133060 None:intergenic
CTCTAAGAATAGGCGTGTCG+CGG 0.611181 8_1:-22132605 Msa1185240:three_prime_UTR
CAAAAGCAGTTACCTGTGAA+CGG 0.629108 8_1:+22133799 None:intergenic
TCATAACAGTCTATAAACCA+TGG 0.635600 8_1:-22132632 Msa1185240:three_prime_UTR
AAGCAGTTACCTGTGAACGG+CGG 0.765283 8_1:+22133802 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAACATAAGTTTTTATAATA+TGG + chr8_1:22133133-22133152 None:intergenic 10.0%
!! GATTTATTTATGTAATTTAT+AGG - chr8_1:22133591-22133610 Msa1185240:intron 10.0%
!!! TAACATAAGTTTTTATAATA+TGG + chr8_1:22133133-22133152 None:intergenic 10.0%
!! GATTTATTTATGTAATTTAT+AGG - chr8_1:22133591-22133610 Msa1185240:intron 10.0%
!!! TACAAAATTTTGATTTGTTT+AGG - chr8_1:22133031-22133050 Msa1185240:CDS 15.0%
!! AAAAATACTAACATAAATTG+TGG + chr8_1:22133077-22133096 None:intergenic 15.0%
!!! TACAAAATTTTGATTTGTTT+AGG - chr8_1:22133031-22133050 Msa1185240:CDS 15.0%
!! AAAAATACTAACATAAATTG+TGG + chr8_1:22133077-22133096 None:intergenic 15.0%
!!! TTCAATTGTATTTGATTTCT+AGG - chr8_1:22132673-22132692 Msa1185240:three_prime_UTR 20.0%
!!! TAAGTTTTTATAATATGGTG+AGG + chr8_1:22133128-22133147 None:intergenic 20.0%
!! AACTTATGTTAGTATGAAAT+TGG - chr8_1:22133142-22133161 Msa1185240:intron 20.0%
!! GATAAATTAGTATAATCAAG+TGG - chr8_1:22133483-22133502 Msa1185240:intron 20.0%
!! ATAAATATTACTATAGGCAT+AGG - chr8_1:22133954-22133973 Msa1185240:intron 20.0%
!!! TTCAATTGTATTTGATTTCT+AGG - chr8_1:22132673-22132692 Msa1185240:three_prime_UTR 20.0%
!!! TAAGTTTTTATAATATGGTG+AGG + chr8_1:22133128-22133147 None:intergenic 20.0%
!! AACTTATGTTAGTATGAAAT+TGG - chr8_1:22133142-22133161 Msa1185240:intron 20.0%
!! GATAAATTAGTATAATCAAG+TGG - chr8_1:22133483-22133502 Msa1185240:intron 20.0%
!! ATAAATATTACTATAGGCAT+AGG - chr8_1:22133954-22133973 Msa1185240:intron 20.0%
!! TCAAACTCAATTTGAAATGT+TGG + chr8_1:22133192-22133211 None:intergenic 25.0%
! CAAACTAGTTACAATTAAAG+GGG + chr8_1:22133235-22133254 None:intergenic 25.0%
! CCAAACTAGTTACAATTAAA+GGG + chr8_1:22133236-22133255 None:intergenic 25.0%
! TCCAAACTAGTTACAATTAA+AGG + chr8_1:22133237-22133256 None:intergenic 25.0%
! TTCTAACCACATTATTTGAA+TGG + chr8_1:22133283-22133302 None:intergenic 25.0%
! AGTGGCTATAATTATACATT+TGG - chr8_1:22133501-22133520 Msa1185240:intron 25.0%
!! TTATAGGACTTTGTTATGAT+TGG - chr8_1:22133607-22133626 Msa1185240:intron 25.0%
! ACAAAAAATACATTCTGTCT+CGG + chr8_1:22133669-22133688 None:intergenic 25.0%
! TTTAATAACACAGTGAAGAT+TGG + chr8_1:22133813-22133832 None:intergenic 25.0%
!! TTAAAAGCTGAATTTTGTGA+TGG - chr8_1:22133850-22133869 Msa1185240:CDS 25.0%
! AAAGCATAGCTATTATACAA+AGG + chr8_1:22133900-22133919 None:intergenic 25.0%
!!! ATGTATGATGTTGTTGTTTA+TGG - chr8_1:22134026-22134045 Msa1185240:intron 25.0%
!! TCAAACTCAATTTGAAATGT+TGG + chr8_1:22133192-22133211 None:intergenic 25.0%
! CAAACTAGTTACAATTAAAG+GGG + chr8_1:22133235-22133254 None:intergenic 25.0%
! CCAAACTAGTTACAATTAAA+GGG + chr8_1:22133236-22133255 None:intergenic 25.0%
! TCCAAACTAGTTACAATTAA+AGG + chr8_1:22133237-22133256 None:intergenic 25.0%
! TTCTAACCACATTATTTGAA+TGG + chr8_1:22133283-22133302 None:intergenic 25.0%
! AGTGGCTATAATTATACATT+TGG - chr8_1:22133501-22133520 Msa1185240:intron 25.0%
!! TTATAGGACTTTGTTATGAT+TGG - chr8_1:22133607-22133626 Msa1185240:intron 25.0%
! ACAAAAAATACATTCTGTCT+CGG + chr8_1:22133669-22133688 None:intergenic 25.0%
! TTTAATAACACAGTGAAGAT+TGG + chr8_1:22133813-22133832 None:intergenic 25.0%
!! TTAAAAGCTGAATTTTGTGA+TGG - chr8_1:22133850-22133869 Msa1185240:CDS 25.0%
! AAAGCATAGCTATTATACAA+AGG + chr8_1:22133900-22133919 None:intergenic 25.0%
!!! ATGTATGATGTTGTTGTTTA+TGG - chr8_1:22134026-22134045 Msa1185240:intron 25.0%
TCATATGCAATCACAGTAAA+GGG + chr8_1:22132729-22132748 None:intergenic 30.0%
TTCATATGCAATCACAGTAA+AGG + chr8_1:22132730-22132749 None:intergenic 30.0%
TGTGATTGCATATGAATTCT+AGG - chr8_1:22132734-22132753 Msa1185240:CDS 30.0%
ACAGAATTAAGAAATGGTTG+AGG + chr8_1:22132782-22132801 None:intergenic 30.0%
TCAACCATTTCTTAATTCTG+TGG - chr8_1:22132781-22132800 Msa1185240:intron 30.0%
!! ATAATGGTGAGTTGATTCTT+TGG - chr8_1:22132864-22132883 Msa1185240:intron 30.0%
! CTTTGGTAATTTATCACACT+AGG - chr8_1:22132881-22132900 Msa1185240:intron 30.0%
CCCTTTAATTGTAACTAGTT+TGG - chr8_1:22133233-22133252 Msa1185240:intron 30.0%
CTCTAACCATTCAAATAATG+TGG - chr8_1:22133274-22133293 Msa1185240:intron 30.0%
TCTATATGACATAGTAATGC+TGG - chr8_1:22133342-22133361 Msa1185240:intron 30.0%
TTCAAGAAATTGTCTTCAGT+TGG - chr8_1:22133918-22133937 Msa1185240:intron 30.0%
!! ATGTTTAACTTTTGCAGAAG+TGG - chr8_1:22133988-22134007 Msa1185240:intron 30.0%
TCATAACAGTCTATAAACCA+TGG - chr8_1:22134118-22134137 Msa1185240:intron 30.0%
TCATATGCAATCACAGTAAA+GGG + chr8_1:22132729-22132748 None:intergenic 30.0%
TTCATATGCAATCACAGTAA+AGG + chr8_1:22132730-22132749 None:intergenic 30.0%
TGTGATTGCATATGAATTCT+AGG - chr8_1:22132734-22132753 Msa1185240:CDS 30.0%
ACAGAATTAAGAAATGGTTG+AGG + chr8_1:22132782-22132801 None:intergenic 30.0%
TCAACCATTTCTTAATTCTG+TGG - chr8_1:22132781-22132800 Msa1185240:intron 30.0%
!! ATAATGGTGAGTTGATTCTT+TGG - chr8_1:22132864-22132883 Msa1185240:intron 30.0%
! CTTTGGTAATTTATCACACT+AGG - chr8_1:22132881-22132900 Msa1185240:intron 30.0%
CCCTTTAATTGTAACTAGTT+TGG - chr8_1:22133233-22133252 Msa1185240:intron 30.0%
CTCTAACCATTCAAATAATG+TGG - chr8_1:22133274-22133293 Msa1185240:intron 30.0%
TCTATATGACATAGTAATGC+TGG - chr8_1:22133342-22133361 Msa1185240:intron 30.0%
TTCAAGAAATTGTCTTCAGT+TGG - chr8_1:22133918-22133937 Msa1185240:intron 30.0%
!! ATGTTTAACTTTTGCAGAAG+TGG - chr8_1:22133988-22134007 Msa1185240:intron 30.0%
TCATAACAGTCTATAAACCA+TGG - chr8_1:22134118-22134137 Msa1185240:intron 30.0%
CATATGCAATCACAGTAAAG+GGG + chr8_1:22132728-22132747 None:intergenic 35.0%
CATTTCTTAATTCTGTGGCT+GGG - chr8_1:22132786-22132805 Msa1185240:intron 35.0%
ATTTCTTAATTCTGTGGCTG+GGG - chr8_1:22132787-22132806 Msa1185240:intron 35.0%
AGTCTGTTGCTGAAACATAA+TGG - chr8_1:22132848-22132867 Msa1185240:intron 35.0%
AGAGTCATATGCTTAAGATG+TGG - chr8_1:22133312-22133331 Msa1185240:intron 35.0%
TTGTAAACTAACCCATGCAA+AGG + chr8_1:22133403-22133422 None:intergenic 35.0%
ATCCATATGCATCACACATA+AGG + chr8_1:22133560-22133579 None:intergenic 35.0%
TGCATATGGATTTCAAACTG+TGG - chr8_1:22133569-22133588 Msa1185240:intron 35.0%
AAAAGAGACTGAAAACTTCG+CGG - chr8_1:22133723-22133742 Msa1185240:intron 35.0%
! AGTTTGCCTTAGGATGTTTT+TGG + chr8_1:22133771-22133790 None:intergenic 35.0%
AAATGATCGAAGTTTGCCTT+AGG + chr8_1:22133781-22133800 None:intergenic 35.0%
GTTGTTTATGGAGCAAATGT+AGG - chr8_1:22134038-22134057 Msa1185240:intron 35.0%
CATATGCAATCACAGTAAAG+GGG + chr8_1:22132728-22132747 None:intergenic 35.0%
CATTTCTTAATTCTGTGGCT+GGG - chr8_1:22132786-22132805 Msa1185240:intron 35.0%
ATTTCTTAATTCTGTGGCTG+GGG - chr8_1:22132787-22132806 Msa1185240:intron 35.0%
AGTCTGTTGCTGAAACATAA+TGG - chr8_1:22132848-22132867 Msa1185240:intron 35.0%
AGAGTCATATGCTTAAGATG+TGG - chr8_1:22133312-22133331 Msa1185240:intron 35.0%
TTGTAAACTAACCCATGCAA+AGG + chr8_1:22133403-22133422 None:intergenic 35.0%
ATCCATATGCATCACACATA+AGG + chr8_1:22133560-22133579 None:intergenic 35.0%
TGCATATGGATTTCAAACTG+TGG - chr8_1:22133569-22133588 Msa1185240:intron 35.0%
AAAAGAGACTGAAAACTTCG+CGG - chr8_1:22133723-22133742 Msa1185240:intron 35.0%
! AGTTTGCCTTAGGATGTTTT+TGG + chr8_1:22133771-22133790 None:intergenic 35.0%
AAATGATCGAAGTTTGCCTT+AGG + chr8_1:22133781-22133800 None:intergenic 35.0%
GTTGTTTATGGAGCAAATGT+AGG - chr8_1:22134038-22134057 Msa1185240:intron 35.0%
! ATCTCTTCTGTGTTACTCTC+AGG - chr8_1:22132614-22132633 Msa1185240:three_prime_UTR 40.0%
CACAGTAAAGGGGAATTCAA+CGG + chr8_1:22132718-22132737 None:intergenic 40.0%
CCAGCCACAGAATTAAGAAA+TGG + chr8_1:22132788-22132807 None:intergenic 40.0%
CCATTTCTTAATTCTGTGGC+TGG - chr8_1:22132785-22132804 Msa1185240:intron 40.0%
CAAAAGCAGTTACCTGTGAA+CGG + chr8_1:22132954-22132973 None:intergenic 40.0%
AGTCATCTTGAACCTTTGCA+TGG - chr8_1:22133388-22133407 Msa1185240:intron 40.0%
GTCATCTTGAACCTTTGCAT+GGG - chr8_1:22133389-22133408 Msa1185240:intron 40.0%
TGCCTTATGTGTGATGCATA+TGG - chr8_1:22133555-22133574 Msa1185240:intron 40.0%
! GCGAAGTTTTCAGTCTCTTT+TGG + chr8_1:22133724-22133743 None:intergenic 40.0%
! GGCAAACTTCGATCATTTTC+AGG - chr8_1:22133783-22133802 Msa1185240:intron 40.0%
TGTTTACAGCTGAAATCAGC+TGG + chr8_1:22134072-22134091 None:intergenic 40.0%
! AGCTGATTTCAGCTGTAAAC+AGG - chr8_1:22134071-22134090 Msa1185240:intron 40.0%
CAGCTGTAAACAGGTGATTA+TGG - chr8_1:22134080-22134099 Msa1185240:intron 40.0%
! ATCTCTTCTGTGTTACTCTC+AGG - chr8_1:22132614-22132633 Msa1185240:three_prime_UTR 40.0%
CACAGTAAAGGGGAATTCAA+CGG + chr8_1:22132718-22132737 None:intergenic 40.0%
CCAGCCACAGAATTAAGAAA+TGG + chr8_1:22132788-22132807 None:intergenic 40.0%
CCATTTCTTAATTCTGTGGC+TGG - chr8_1:22132785-22132804 Msa1185240:intron 40.0%
CAAAAGCAGTTACCTGTGAA+CGG + chr8_1:22132954-22132973 None:intergenic 40.0%
AGTCATCTTGAACCTTTGCA+TGG - chr8_1:22133388-22133407 Msa1185240:intron 40.0%
GTCATCTTGAACCTTTGCAT+GGG - chr8_1:22133389-22133408 Msa1185240:intron 40.0%
TGCCTTATGTGTGATGCATA+TGG - chr8_1:22133555-22133574 Msa1185240:intron 40.0%
! GCGAAGTTTTCAGTCTCTTT+TGG + chr8_1:22133724-22133743 None:intergenic 40.0%
! GGCAAACTTCGATCATTTTC+AGG - chr8_1:22133783-22133802 Msa1185240:intron 40.0%
TGTTTACAGCTGAAATCAGC+TGG + chr8_1:22134072-22134091 None:intergenic 40.0%
! AGCTGATTTCAGCTGTAAAC+AGG - chr8_1:22134071-22134090 Msa1185240:intron 40.0%
CAGCTGTAAACAGGTGATTA+TGG - chr8_1:22134080-22134099 Msa1185240:intron 40.0%
CAATGCTCATGAGCTTCTCA+AGG + chr8_1:22133693-22133712 None:intergenic 45.0%
CGCGGCATCATTGTCTAAAT+TGG - chr8_1:22133741-22133760 Msa1185240:intron 45.0%
GGCTTGCCAAAAACATCCTA+AGG - chr8_1:22133762-22133781 Msa1185240:intron 45.0%
CAGAAGTGGCTTGAGAAGAA+TGG - chr8_1:22134002-22134021 Msa1185240:intron 45.0%
!! CCTATTCTTAGAGGTGTCCA+TGG + chr8_1:22134138-22134157 None:intergenic 45.0%
! CCATGGACACCTCTAAGAAT+AGG - chr8_1:22134135-22134154 Msa1185240:intron 45.0%
CAATGCTCATGAGCTTCTCA+AGG + chr8_1:22133693-22133712 None:intergenic 45.0%
CGCGGCATCATTGTCTAAAT+TGG - chr8_1:22133741-22133760 Msa1185240:intron 45.0%
GGCTTGCCAAAAACATCCTA+AGG - chr8_1:22133762-22133781 Msa1185240:intron 45.0%
CAGAAGTGGCTTGAGAAGAA+TGG - chr8_1:22134002-22134021 Msa1185240:intron 45.0%
!! CCTATTCTTAGAGGTGTCCA+TGG + chr8_1:22134138-22134157 None:intergenic 45.0%
! CCATGGACACCTCTAAGAAT+AGG - chr8_1:22134135-22134154 Msa1185240:intron 45.0%
!! TAATATATAAATATTACTAT+AGG - chr8_1:22133948-22133967 Msa1185240:intron 5.0%
!! TAATATATAAATATTACTAT+AGG - chr8_1:22133948-22133967 Msa1185240:intron 5.0%
AAGCAGTTACCTGTGAACGG+CGG + chr8_1:22132951-22132970 None:intergenic 50.0%
! CTCTAAGAATAGGCGTGTCG+CGG - chr8_1:22134145-22134164 Msa1185240:CDS 50.0%
AAGCAGTTACCTGTGAACGG+CGG + chr8_1:22132951-22132970 None:intergenic 50.0%
! CTCTAAGAATAGGCGTGTCG+CGG - chr8_1:22134145-22134164 Msa1185240:CDS 50.0%
CGCGACACGCCTATTCTTAG+AGG + chr8_1:22134147-22134166 None:intergenic 55.0%
CGCGACACGCCTATTCTTAG+AGG + chr8_1:22134147-22134166 None:intergenic 55.0%
TCATTCTCGCCGCCGTTCAC+AGG - chr8_1:22132939-22132958 Msa1185240:intron 60.0%
TCATTCTCGCCGCCGTTCAC+AGG - chr8_1:22132939-22132958 Msa1185240:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 22132595 22134177 22132595 ID=Msa1185240;Name=Msa1185240
chr8_1 mRNA 22132595 22134177 22132595 ID=Msa1185240-mRNA-1;Parent=Msa1185240;Name=Msa1185240-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0.33|0.25|0.5|1|0.75|4|101|120
chr8_1 exon 22132595 22132767 22132595 ID=Msa1185240-mRNA-1:exon:8214;Parent=Msa1185240-mRNA-1
chr8_1 exon 22132968 22133159 22132968 ID=Msa1185240-mRNA-1:exon:8213;Parent=Msa1185240-mRNA-1
chr8_1 exon 22133812 22133869 22133812 ID=Msa1185240-mRNA-1:exon:8212;Parent=Msa1185240-mRNA-1
chr8_1 exon 22134137 22134177 22134137 ID=Msa1185240-mRNA-1:exon:8211;Parent=Msa1185240-mRNA-1
chr8_1 CDS 22134137 22134177 22134137 ID=Msa1185240-mRNA-1:cds;Parent=Msa1185240-mRNA-1
chr8_1 CDS 22133812 22133869 22133812 ID=Msa1185240-mRNA-1:cds;Parent=Msa1185240-mRNA-1
chr8_1 CDS 22132968 22133159 22132968 ID=Msa1185240-mRNA-1:cds;Parent=Msa1185240-mRNA-1
chr8_1 CDS 22132696 22132767 22132696 ID=Msa1185240-mRNA-1:cds;Parent=Msa1185240-mRNA-1
chr8_1 three_prime_UTR 22132595 22132695 22132595 ID=Msa1185240-mRNA-1:three_prime_utr;Parent=Msa1185240-mRNA-1
Gene Sequence

>Msa1185240

ATGATCAACACTATCTTTGATCTCTTCTGTGTTACTCTCAGGTGTTCACAAGTAACTGCTCTCAATCATTCTTATCATCATTCTCGCCGCCGTTCACAGGACTTTGTTATGATTGGTTTCAATAGAATTTCATTTCAGTTCATGACATATTGCCGAGACAGAATGTATTTTTTGTGCCTTGAGAAGCTCATGAGCATTGATATTGATCCAAAAGAGACTGAAAACTTCGCGGCATCATTGTCTAAATTGGCTTGCCAAAAACATCCTAAGGCAAACTTCGATCATTTTCAGAAGTGGCTTGAGAAGAATGGCATGTATGATGTTGTTGTTTATGGAGCAAATGTAGGACTTGCAACCAGCTGA

Protein sequence

>Msa1185240

MINTIFDLFCVTLRCSQVTALNHSYHHSRRRSQDFVMIGFNRISFQFMTYCRDRMYFLCLEKLMSIDIDPKETENFAASLSKLACQKHPKANFDHFQKWLEKNGMYDVVVYGANVGLATS*