Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1187410 | sp|Q8GYA4|CRK10_ARATH | 56.119 | 335 | 141 | 3 | 2 | 331 | 336 | 669 | 1.32e-133 | 398 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0806090 | Msa1187410 | 0.811810 | 5.811334e-51 | -8.615850e-47 |
| Msa0898380 | Msa1187410 | 0.819642 | 1.043454e-52 | -8.615850e-47 |
| Msa0951450 | Msa1187410 | 0.802205 | 6.264872e-49 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1187410 | MtrunA17_Chr8g0354281 | 96.364 | 330 | 12 | 0 | 2 | 331 | 324 | 653 | 0.0 | 669 |
| Msa1187410 | MtrunA17_Chr8g0354351 | 81.627 | 332 | 60 | 1 | 1 | 331 | 321 | 652 | 0.0 | 574 |
| Msa1187410 | MtrunA17_Chr8g0354421 | 81.571 | 331 | 60 | 1 | 2 | 331 | 352 | 682 | 0.0 | 566 |
| Msa1187410 | MtrunA17_Chr8g0354441 | 81.571 | 331 | 60 | 1 | 2 | 331 | 339 | 669 | 0.0 | 564 |
| Msa1187410 | MtrunA17_Chr8g0354431 | 81.325 | 332 | 61 | 1 | 1 | 331 | 330 | 661 | 0.0 | 564 |
| Msa1187410 | MtrunA17_Chr8g0354331 | 79.217 | 332 | 68 | 1 | 1 | 331 | 88 | 419 | 0.0 | 557 |
| Msa1187410 | MtrunA17_Chr3g0081001 | 73.494 | 332 | 87 | 1 | 1 | 331 | 331 | 662 | 1.06e-180 | 515 |
| Msa1187410 | MtrunA17_Chr3g0081011 | 73.193 | 332 | 88 | 1 | 1 | 331 | 327 | 658 | 8.20e-179 | 510 |
| Msa1187410 | MtrunA17_Chr3g0082941 | 71.299 | 331 | 87 | 1 | 1 | 331 | 161 | 483 | 3.95e-178 | 502 |
| Msa1187410 | MtrunA17_Chr8g0354271 | 74.426 | 305 | 77 | 1 | 2 | 305 | 324 | 628 | 1.12e-171 | 491 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1187410 | AT4G23180.1 | 56.119 | 335 | 141 | 3 | 2 | 331 | 336 | 669 | 1.34e-134 | 398 |
| Msa1187410 | AT4G21390.1 | 63.158 | 304 | 111 | 1 | 1 | 304 | 516 | 818 | 2.78e-133 | 400 |
| Msa1187410 | AT4G23140.1 | 55.786 | 337 | 141 | 2 | 2 | 331 | 339 | 674 | 1.13e-132 | 393 |
| Msa1187410 | AT4G23160.1 | 59.091 | 308 | 126 | 0 | 4 | 311 | 349 | 656 | 1.25e-132 | 393 |
| Msa1187410 | AT4G23270.2 | 56.364 | 330 | 142 | 2 | 4 | 331 | 316 | 645 | 8.08e-132 | 390 |
| Msa1187410 | AT4G23270.1 | 56.364 | 330 | 142 | 2 | 4 | 331 | 316 | 645 | 8.08e-132 | 390 |
| Msa1187410 | AT4G23230.1 | 58.804 | 301 | 124 | 0 | 2 | 302 | 325 | 625 | 1.84e-131 | 388 |
| Msa1187410 | AT4G23130.1 | 54.545 | 330 | 148 | 2 | 4 | 331 | 330 | 659 | 3.30e-131 | 389 |
| Msa1187410 | AT4G23130.2 | 54.545 | 330 | 148 | 2 | 4 | 331 | 334 | 663 | 3.86e-131 | 389 |
| Msa1187410 | AT1G65790.1 | 57.143 | 329 | 134 | 3 | 10 | 331 | 515 | 843 | 3.31e-130 | 392 |
Find 56 sgRNAs with CRISPR-Local
Find 244 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAGATGATACATTAAA+TGG | 0.267829 | 8_1:-27112667 | Msa1187410:five_prime_UTR |
| AAAATCATACATTGAAAGTG+AGG | 0.595294 | 8_1:-27111016 | Msa1187410:CDS |
| AAAGGCTTGCAGAAACGTCG+AGG | 0.674797 | 8_1:-27112403 | Msa1187410:CDS |
| AACAACATAAGCAACTAGAT+TGG | 0.459558 | 8_1:-27111917 | Msa1187410:CDS |
| AACGTCGAGGCAAGGTTCAG+AGG | 0.542811 | 8_1:-27112390 | Msa1187410:CDS |
| AACTCCAAAGCTGAAAACAT+CGG | 0.629248 | 8_1:+27111539 | None:intergenic |
| AACTGAAAATGCCGGTTGCT+TGG | 0.371728 | 8_1:+27110879 | None:intergenic |
| AAGTTAGGAGAAGGTGGCTT+TGG | 0.430046 | 8_1:-27112580 | Msa1187410:CDS |
| AATCCAGCCATAGCATATTC+TGG | 0.348026 | 8_1:+27111576 | None:intergenic |
| AATGCCGGTTGCTTGGGTTT+TGG | 0.332035 | 8_1:+27110886 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTACAGAAAAAGGGATCAT+AGG | + | chr8_1:27110825-27110844 | None:intergenic | 30.0% | |
| GCATGTTGAATTACAGAAAA+AGG | + | chr8_1:27110834-27110853 | None:intergenic | 30.0% | |
| TTCATTCATACCTTGAAAAC+AGG | + | chr8_1:27110906-27110925 | None:intergenic | 30.0% | |
| GTATATATGCATAGGAAACT+TGG | + | chr8_1:27110942-27110961 | None:intergenic | 30.0% | |
| TGAAATTCAAGGCTTGAATT+CGG | + | chr8_1:27111205-27111224 | None:intergenic | 30.0% | |
| TATGTCAAAACATACCGAAA+AGG | + | chr8_1:27111228-27111247 | None:intergenic | 30.0% | |
| TCTTATAGTTCGAACTAACT+TGG | + | chr8_1:27111300-27111319 | None:intergenic | 30.0% | |
| AGAAGTTACTATATGTCTAG+CGG | - | chr8_1:27111317-27111336 | Msa1187410:intron | 30.0% | |
| ATGTTGTTCTTCATCTAACA+TGG | + | chr8_1:27111530-27111549 | None:intergenic | 30.0% | |
| AACAACATAAGCAACTAGAT+TGG | - | chr8_1:27111542-27111561 | Msa1187410:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 27110789 | 27112692 | 27110789 | ID=Msa1187410;Name=Msa1187410 |
| chr8_1 | mRNA | 27110789 | 27112692 | 27110789 | ID=Msa1187410-mRNA-1;Parent=Msa1187410;Name=Msa1187410-mRNA-1;_AED=0.37;_eAED=0.37;_QI=36|0.75|0.8|1|0.75|0.4|5|0|331 |
| chr8_1 | exon | 27110789 | 27111094 | 27110789 | ID=Msa1187410-mRNA-1:exon:9536;Parent=Msa1187410-mRNA-1 |
| chr8_1 | exon | 27111461 | 27111611 | 27111461 | ID=Msa1187410-mRNA-1:exon:9535;Parent=Msa1187410-mRNA-1 |
| chr8_1 | exon | 27111708 | 27111945 | 27111708 | ID=Msa1187410-mRNA-1:exon:9534;Parent=Msa1187410-mRNA-1 |
| chr8_1 | exon | 27112249 | 27112459 | 27112249 | ID=Msa1187410-mRNA-1:exon:9533;Parent=Msa1187410-mRNA-1 |
| chr8_1 | exon | 27112567 | 27112692 | 27112567 | ID=Msa1187410-mRNA-1:exon:9532;Parent=Msa1187410-mRNA-1 |
| chr8_1 | five_prime_UTR | 27112657 | 27112692 | 27112657 | ID=Msa1187410-mRNA-1:five_prime_utr;Parent=Msa1187410-mRNA-1 |
| chr8_1 | CDS | 27112567 | 27112656 | 27112567 | ID=Msa1187410-mRNA-1:cds;Parent=Msa1187410-mRNA-1 |
| chr8_1 | CDS | 27112249 | 27112459 | 27112249 | ID=Msa1187410-mRNA-1:cds;Parent=Msa1187410-mRNA-1 |
| chr8_1 | CDS | 27111708 | 27111945 | 27111708 | ID=Msa1187410-mRNA-1:cds;Parent=Msa1187410-mRNA-1 |
| chr8_1 | CDS | 27111461 | 27111611 | 27111461 | ID=Msa1187410-mRNA-1:cds;Parent=Msa1187410-mRNA-1 |
| chr8_1 | CDS | 27110789 | 27111094 | 27110789 | ID=Msa1187410-mRNA-1:cds;Parent=Msa1187410-mRNA-1 |
| Gene Sequence |
>Msa1187410 ATGATCCCTTTTTCTGTAATTCAACATGCCACTAATTACTTTTCATCATCAGCAAAGTTAGGAGAAGGTGGCTTTGGACCTGTTTTCAAGGGTACTTTACCAGATGGGACAGAAATTGCAGTCAAAAGGCTTGCAGAAACGTCGAGGCAAGGTTCAGAGGAGTTCAAGAATGAAGTAATCTTCATAGCTAAACTGCAACACAGAAACCTTGTGAGACTCTTGGGTTGCTGTATTAAAGGAAATGAAAAGATACTGGTATATGAATACATGCCGAATTCAAGCCTTGAATTTCACCTTTTCGATGAAGAACAACATAAGCAACTAGATTGGACATTACGACTAAGTATTATCAATGGTATCGCGAGAGGACTTTTATACCTTCACCAGGATTCTCGACTTAGAGTTATTCATAGAGACCTTAAAGCCAGCAACGTTCTTCTAGATGATGAAATGAATCCAAAAATATCAGATTTTGGATTGGCAAGGAAATTTGAAAAAGGTCAAAGTCAGACAAAAACAAAAAGAGTTATTGGCACCTACGGATACATGGCTCCAGAATATGCTATGGCTGGATTATTTTCAGTGAAATCCGATGTTTTCAGCTTTGGAGTTCTTTTATTAGAAGTTGTATATGGAAAAAGGAACGGTGAATTAGTTCTTTCAGAACATGGGCAAAGTCTTCTATTATATACTTGGAAGCTTTGGTGTGAAGGAAAAAGTTTGGAATTAATTGATCCAATCCACAAAAAATCATACATTGAAAGTGAGGTTATGAAGTGCATACACATTGGTCTATTGTGTGTACAACAAGATGCTGCAGATCGACCAACCATGTCCACCGTCGTTGCTATGCTAGGCAGTGACACAATGCCCATTCCAAAACCCAAGCAACCGGCATTTTCAGTTGGAAGAATTACGGAGGAAGATCCAACTTTAAAAAGTTATAAGGATAATTATGTTGATGAAGTACCCATAACTATTGTTTCACCTAGATAG |
| Protein sequence |
>Msa1187410 MIPFSVIQHATNYFSSSAKLGEGGFGPVFKGTLPDGTEIAVKRLAETSRQGSEEFKNEVIFIAKLQHRNLVRLLGCCIKGNEKILVYEYMPNSSLEFHLFDEEQHKQLDWTLRLSIINGIARGLLYLHQDSRLRVIHRDLKASNVLLDDEMNPKISDFGLARKFEKGQSQTKTKRVIGTYGYMAPEYAMAGLFSVKSDVFSFGVLLLEVVYGKRNGELVLSEHGQSLLLYTWKLWCEGKSLELIDPIHKKSYIESEVMKCIHIGLLCVQQDAADRPTMSTVVAMLGSDTMPIPKPKQPAFSVGRITEEDPTLKSYKDNYVDEVPITIVSPR* |
