AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1194410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1194410 MtrunA17_Chr8g0364261 100.000 87 0 0 1 87 1 87 3.40e-63 185
Msa1194410 MtrunA17_Chr3g0084311 97.701 87 2 0 1 87 1 87 1.08e-61 181
Msa1194410 MtrunA17_Chr3g0084301 97.701 87 2 0 1 87 1 87 1.08e-61 181
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1194410 AT4G14342.1 91.954 87 7 0 1 87 1 87 2.36e-59 176
Msa1194410 AT3G23325.1 89.655 87 9 0 1 87 1 87 2.98e-58 173
Msa1194410 AT4G14342.2 86.250 80 11 0 1 80 1 80 3.44e-49 150

Find 37 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTACTCTTCTTCTCTTTC+GGG 0.158502 8_1:-41589914 None:intergenic
CTAATTTATATGTTAGCTTT+TGG 0.228345 8_1:-41590240 None:intergenic
ATCCTACTCTTCTTCTCTTT+CGG 0.235938 8_1:-41589915 None:intergenic
AAGAAGAGTAGGATTATTGT+TGG 0.374006 8_1:+41589924 Msa1194410:exon
GGTTGTAACCTACTAGTACT+AGG 0.384785 8_1:+41590180 Msa1194410:three_prime_UTR
ATTATTGTTGGGATTACAAA+AGG 0.418300 8_1:+41589936 Msa1194410:three_prime_UTR
CAAAATTCCCTAGTACTAGT+AGG 0.418351 8_1:-41590188 None:intergenic
CGCGAGTAGAGTAGGGTTTG+AGG 0.433467 8_1:+41587466 Msa1194410:five_prime_UTR
ATCGCTCAAAACGAATCTAT+TGG 0.434978 8_1:+41587783 Msa1194410:CDS
TGGAAAAGTAAGAAAGTAAA+GGG 0.449644 8_1:-41587761 None:intergenic
CAAGCTAAATACGTTGGAAC+TGG 0.455380 8_1:+41587663 Msa1194410:CDS
AGCTTGAAGATGTTCAAGTT+GGG 0.460543 8_1:-41587646 None:intergenic
AGTTACTTAGTTTATAAAAG+AGG 0.462906 8_1:+41587395 None:intergenic
ATGGAAAAGTAAGAAAGTAA+AGG 0.463000 8_1:-41587762 None:intergenic
CTGATATCAACAGATTTGAA+TGG 0.473717 8_1:+41587691 Msa1194410:CDS
TAGCTTGAAGATGTTCAAGT+TGG 0.478107 8_1:-41587647 None:intergenic
AGAAGAGTAGGATTATTGTT+GGG 0.483219 8_1:+41589925 Msa1194410:exon
TAGCAAGACGCGAGTAGAGT+AGG 0.483904 8_1:+41587458 Msa1194410:five_prime_UTR
AGTAAGAAAGTAAAGGGTAA+TGG 0.502423 8_1:-41587755 None:intergenic
GTTGTAACCTACTAGTACTA+GGG 0.505994 8_1:+41590181 Msa1194410:three_prime_UTR
GTTACTTAGTTTATAAAAGA+GGG 0.510094 8_1:+41587396 None:intergenic
GCGAGTAGAGTAGGGTTTGA+GGG 0.518608 8_1:+41587467 Msa1194410:five_prime_UTR
AGCAAGACGCGAGTAGAGTA+GGG 0.530853 8_1:+41587459 Msa1194410:five_prime_UTR
GACCGCTCGTTGTACTTGTA+CGG 0.533230 8_1:+41589986 Msa1194410:three_prime_UTR
GATAGCTATGCTTCATACAT+TGG 0.550538 8_1:+41587732 Msa1194410:CDS
TGATATCAACAGATTTGAAT+GGG 0.559675 8_1:+41587692 Msa1194410:CDS
TTAGTTTATAAAAGAGGGAG+CGG 0.584238 8_1:+41587401 Msa1194410:five_prime_UTR
ATAAACGAGAGGCAATATGC+AGG 0.585212 8_1:+41587499 Msa1194410:exon
CATCTTCAAGCTAAATACGT+TGG 0.585829 8_1:+41587657 Msa1194410:CDS
CTTTCGGGAGGCAGACCACA+AGG 0.594210 8_1:-41589899 None:intergenic
TGTACGGTACAATCTGATTG+AGG 0.597919 8_1:+41590002 Msa1194410:three_prime_UTR
TACTCTTCTTCTCTTTCGGG+AGG 0.605128 8_1:-41589911 None:intergenic
GTTTATAAAAGAGGGAGCGG+TGG 0.609994 8_1:+41587404 Msa1194410:five_prime_UTR
TTCTACAACTCATTGTCTCG+TGG 0.611072 8_1:-41590126 None:intergenic
TCCCGAAAGAGAAGAAGAGT+AGG 0.611906 8_1:+41589913 Msa1194410:CDS
GGACGAAGAAAATAAACGAG+AGG 0.675984 8_1:+41587488 Msa1194410:five_prime_UTR
TACCGTACAAGTACAACGAG+CGG 0.693261 8_1:-41589988 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTTTATTAGAAATAAAT+AGG - chr8_1:41588284-41588303 None:intergenic 10.0%
!!! GTATTAATTATTTTAAAAAC+AGG + chr8_1:41588565-41588584 Msa1194410:intron 10.0%
!!! TTTTTTACTTTTCATTAATT+TGG + chr8_1:41590159-41590178 Msa1194410:three_prime_UTR 10.0%
! GGGGGGGGGGGGGGGGGGGG+GGG - chr8_1:41589413-41589432 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG - chr8_1:41589413-41589432 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG - chr8_1:41589413-41589432 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG - chr8_1:41589413-41589432 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG - chr8_1:41589413-41589432 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG - chr8_1:41589413-41589432 None:intergenic 100.0%
! GGGGGGGGGGGGGGGGGGGG+GGG - chr8_1:41589413-41589432 None:intergenic 100.0%
!!! CTTCTCTTAAAAAATATTTT+TGG - chr8_1:41588961-41588980 None:intergenic 15.0%
!!! ATATTTTTTAAGAGAAGATT+TGG + chr8_1:41588964-41588983 Msa1194410:intron 15.0%
!!! AGTATTTTAAATGTGATTTA+AGG + chr8_1:41589278-41589297 Msa1194410:intron 15.0%
!! TATGAATTTATATGTGTATA+TGG + chr8_1:41589330-41589349 Msa1194410:intron 15.0%
!! ATGAATTTATATGTGTATAT+GGG + chr8_1:41589331-41589350 Msa1194410:intron 15.0%
!! AATAAAAATTGAATAAACCA+AGG - chr8_1:41589757-41589776 None:intergenic 15.0%
!!! TAATTTATATGTTAGCTTTT+GGG - chr8_1:41590242-41590261 None:intergenic 15.0%
!! AATTTAACGAAATCATGAAA+CGG + chr8_1:41587580-41587599 Msa1194410:intron 20.0%
!!! TTCCTATGATTTTTAAATCA+CGG - chr8_1:41588147-41588166 None:intergenic 20.0%
!!! ATTTTTTAAAGATAGCAATC+TGG - chr8_1:41588306-41588325 None:intergenic 20.0%
!!! AATAATCCTATTTTCAAACT+GGG - chr8_1:41588514-41588533 None:intergenic 20.0%
!!! AAATAATCCTATTTTCAAAC+TGG - chr8_1:41588515-41588534 None:intergenic 20.0%
!! AGTTTGAAAATAGGATTATT+TGG + chr8_1:41588514-41588533 Msa1194410:intron 20.0%
!! ATTCTTCTTAAACATGTTAA+TGG - chr8_1:41589004-41589023 None:intergenic 20.0%
!! AATTAAAATTCGTAAGCATA+AGG - chr8_1:41589252-41589271 None:intergenic 20.0%
!!! TACAGATTTTTATAATGTTG+AGG - chr8_1:41589509-41589528 None:intergenic 20.0%
!! CAACATTATAAAAATCTGTA+TGG + chr8_1:41589509-41589528 Msa1194410:intron 20.0%
!! CATTTGGTTACAAAAATATT+TGG + chr8_1:41589566-41589585 Msa1194410:intron 20.0%
!!! GGTTAATTCAATTCTTTTTT+TGG + chr8_1:41589631-41589650 Msa1194410:intron 20.0%
!!! ATGTGTTATTTTTTGTTAGT+TGG + chr8_1:41589673-41589692 Msa1194410:intron 20.0%
!!! TGTGTTATTTTTTGTTAGTT+GGG + chr8_1:41589674-41589693 Msa1194410:intron 20.0%
!!! ATTAAGAATATAAAGTGCTT+AGG - chr8_1:41589836-41589855 None:intergenic 20.0%
!!! CTAATTTATATGTTAGCTTT+TGG - chr8_1:41590243-41590262 None:intergenic 20.0%
! TGATATCAACAGATTTGAAT+GGG + chr8_1:41587692-41587711 Msa1194410:CDS 25.0%
! TGGAAAAGTAAGAAAGTAAA+GGG - chr8_1:41587764-41587783 None:intergenic 25.0%
! ATGGAAAAGTAAGAAAGTAA+AGG - chr8_1:41587765-41587784 None:intergenic 25.0%
! AACCGTGATTTAAAAATCAT+AGG + chr8_1:41588142-41588161 Msa1194410:intron 25.0%
! AATAAAGATGAAACAGATGA+GGG - chr8_1:41588721-41588740 None:intergenic 25.0%
!!! TAGTCTTTTTTCTATAGCTA+AGG - chr8_1:41588854-41588873 None:intergenic 25.0%
! TGTAGAACTTACTGAAAAAA+GGG + chr8_1:41588902-41588921 Msa1194410:intron 25.0%
! ATGTCTCATCTAATAATGAA+TGG + chr8_1:41589037-41589056 Msa1194410:intron 25.0%
!!! ATCTAATAATGAATGGATTG+TGG + chr8_1:41589044-41589063 Msa1194410:intron 25.0%
! TTTATAACATCGTGTTCAAT+GGG - chr8_1:41589090-41589109 None:intergenic 25.0%
!! TTTTACACAAAATTGGAAAG+GGG - chr8_1:41589441-41589460 None:intergenic 25.0%
!! GTTTTACACAAAATTGGAAA+GGG - chr8_1:41589442-41589461 None:intergenic 25.0%
!! TTATTAGATGAAAAGTATGC+AGG - chr8_1:41589483-41589502 None:intergenic 25.0%
!! AAAATCTGTATGGATTAAAG+TGG + chr8_1:41589519-41589538 Msa1194410:intron 25.0%
! TTGGTTACAAAAATATTTGG+AGG + chr8_1:41589569-41589588 Msa1194410:intron 25.0%
!! AAGAATTGAATTAACCCTTT+TGG - chr8_1:41589627-41589646 None:intergenic 25.0%
!!! GTGTTATTTTTTGTTAGTTG+GGG + chr8_1:41589675-41589694 Msa1194410:intron 25.0%
!!! TGTTATTTTTTGTTAGTTGG+GGG + chr8_1:41589676-41589695 Msa1194410:intron 25.0%
!! TTTTCTGCAACAAAACAAAA+AGG - chr8_1:41589871-41589890 None:intergenic 25.0%
! ATTATTGTTGGGATTACAAA+AGG + chr8_1:41589936-41589955 Msa1194410:three_prime_UTR 25.0%
!! TGTTCATGTTTTTCTTCTTT+CGG + chr8_1:41590049-41590068 Msa1194410:three_prime_UTR 25.0%
!!! CTAAACTTTTGCTGTTTTTA+TGG + chr8_1:41590082-41590101 Msa1194410:three_prime_UTR 25.0%
!!! TTTTGCTGTTTTTATGGTAT+GGG + chr8_1:41590088-41590107 Msa1194410:three_prime_UTR 25.0%
TTAGTTTATAAAAGAGGGAG+CGG + chr8_1:41587401-41587420 Msa1194410:five_prime_UTR 30.0%
GCGAAGAAAAGAGAAAAATT+GGG - chr8_1:41587535-41587554 None:intergenic 30.0%
AGCGAAGAAAAGAGAAAAAT+TGG - chr8_1:41587536-41587555 None:intergenic 30.0%
CTGATATCAACAGATTTGAA+TGG + chr8_1:41587691-41587710 Msa1194410:CDS 30.0%
AGTAAGAAAGTAAAGGGTAA+TGG - chr8_1:41587758-41587777 None:intergenic 30.0%
!! TACTCCATTGATTCATTCAT+GGG - chr8_1:41587909-41587928 None:intergenic 30.0%
!! ATACTCCATTGATTCATTCA+TGG - chr8_1:41587910-41587929 None:intergenic 30.0%
!! GTTAGTTCTGGTTTGTTATT+TGG + chr8_1:41588093-41588112 Msa1194410:intron 30.0%
!! TCTGGTTTGTTATTTGGAAA+TGG + chr8_1:41588099-41588118 Msa1194410:intron 30.0%
AAATCATAGGAAACGATTAG+AGG + chr8_1:41588155-41588174 Msa1194410:intron 30.0%
AATCATAGGAAACGATTAGA+GGG + chr8_1:41588156-41588175 Msa1194410:intron 30.0%
AAGCGATTTAAGAGAGAATT+GGG + chr8_1:41588187-41588206 Msa1194410:intron 30.0%
AGGTGTATTTCAGTTTAAGA+AGG + chr8_1:41588353-41588372 Msa1194410:intron 30.0%
GGTGTATTTCAGTTTAAGAA+GGG + chr8_1:41588354-41588373 Msa1194410:intron 30.0%
AAGATAGTAATCTCTTTCTG+AGG - chr8_1:41588403-41588422 None:intergenic 30.0%
ATAAGACATGTTTATCCATG+CGG + chr8_1:41588475-41588494 Msa1194410:intron 30.0%
AGAAAACCCAGTTTGAAAAT+AGG + chr8_1:41588505-41588524 Msa1194410:intron 30.0%
AAAGTAGCACTAGACAAAAA+TGG - chr8_1:41588660-41588679 None:intergenic 30.0%
GAATAAAGATGAAACAGATG+AGG - chr8_1:41588722-41588741 None:intergenic 30.0%
GTGTAGAACTTACTGAAAAA+AGG + chr8_1:41588901-41588920 Msa1194410:intron 30.0%
!! TAATAATGAATGGATTGTGG+CGG + chr8_1:41589047-41589066 Msa1194410:intron 30.0%
CTTTATAACATCGTGTTCAA+TGG - chr8_1:41589091-41589110 None:intergenic 30.0%
CATTGAACACGATGTTATAA+AGG + chr8_1:41589089-41589108 Msa1194410:intron 30.0%
TTTACACAAAATTGGAAAGG+GGG - chr8_1:41589440-41589459 None:intergenic 30.0%
! GGTTTTACACAAAATTGGAA+AGG - chr8_1:41589443-41589462 None:intergenic 30.0%
! ATCGAGGTTTTACACAAAAT+TGG - chr8_1:41589448-41589467 None:intergenic 30.0%
CAATATCTGAGGAATTCATT+TGG + chr8_1:41589550-41589569 Msa1194410:intron 30.0%
!!! TTTTGGTTCTCCAAAGATTA+GGG - chr8_1:41589610-41589629 None:intergenic 30.0%
! TAATCTTTGGAGAACCAAAA+GGG + chr8_1:41589610-41589629 Msa1194410:intron 30.0%
!!! GTTATTTTTTGTTAGTTGGG+GGG + chr8_1:41589677-41589696 Msa1194410:intron 30.0%
AAGAAGAGTAGGATTATTGT+TGG + chr8_1:41589924-41589943 Msa1194410:exon 30.0%
AGAAGAGTAGGATTATTGTT+GGG + chr8_1:41589925-41589944 Msa1194410:exon 30.0%
!!! CTTTTGCTGTTTTTATGGTA+TGG + chr8_1:41590087-41590106 Msa1194410:three_prime_UTR 30.0%
!!! TTTGCTGTTTTTATGGTATG+GGG + chr8_1:41590089-41590108 Msa1194410:three_prime_UTR 30.0%
!!! TTATATGTTAGCTTTTGGGA+GGG - chr8_1:41590238-41590257 None:intergenic 30.0%
!!! TTTATATGTTAGCTTTTGGG+AGG - chr8_1:41590239-41590258 None:intergenic 30.0%
! AGCTTGAAGATGTTCAAGTT+GGG - chr8_1:41587649-41587668 None:intergenic 35.0%
TAGCTTGAAGATGTTCAAGT+TGG - chr8_1:41587650-41587669 None:intergenic 35.0%
CATCTTCAAGCTAAATACGT+TGG + chr8_1:41587657-41587676 Msa1194410:CDS 35.0%
GATAGCTATGCTTCATACAT+TGG + chr8_1:41587732-41587751 Msa1194410:CDS 35.0%
!! AATAGATTCGTTTTGAGCGA+TGG - chr8_1:41587784-41587803 None:intergenic 35.0%
ATCGCTCAAAACGAATCTAT+TGG + chr8_1:41587783-41587802 Msa1194410:CDS 35.0%
AAGTCCCATGAATGAATCAA+TGG + chr8_1:41587902-41587921 Msa1194410:intron 35.0%
!! TGCAATTTGTTGCTGCAAAT+AGG + chr8_1:41588057-41588076 Msa1194410:intron 35.0%
CAAGCGATTTAAGAGAGAAT+TGG + chr8_1:41588186-41588205 Msa1194410:intron 35.0%
AGCGATTTAAGAGAGAATTG+GGG + chr8_1:41588188-41588207 Msa1194410:intron 35.0%
GGGATATGACTCAATTACAA+AGG - chr8_1:41588263-41588282 None:intergenic 35.0%
TATCTTCTGATTTCTGCTTG+CGG + chr8_1:41588417-41588436 Msa1194410:intron 35.0%
TAAGACATGTTTATCCATGC+GGG + chr8_1:41588476-41588495 Msa1194410:intron 35.0%
CATGAGAAGTAGAAGCAATT+TGG - chr8_1:41588791-41588810 None:intergenic 35.0%
AAAAAGACTAAGAATGCTCC+TGG + chr8_1:41588864-41588883 Msa1194410:intron 35.0%
ACACGATGTTATAAAGGATG+AGG + chr8_1:41589095-41589114 Msa1194410:intron 35.0%
CACGATGTTATAAAGGATGA+GGG + chr8_1:41589096-41589115 Msa1194410:intron 35.0%
! TTCTAGAAGAGATGACGATT+AGG + chr8_1:41589136-41589155 Msa1194410:intron 35.0%
TTACACAAAATTGGAAAGGG+GGG - chr8_1:41589439-41589458 None:intergenic 35.0%
CAGGAAGATAGTTAAAATCG+AGG - chr8_1:41589464-41589483 None:intergenic 35.0%
TATAGTGACACCCTAATCTT+TGG + chr8_1:41589597-41589616 Msa1194410:intron 35.0%
!!! CTTTTGGTTCTCCAAAGATT+AGG - chr8_1:41589611-41589630 None:intergenic 35.0%
! CTAATCTTTGGAGAACCAAA+AGG + chr8_1:41589609-41589628 Msa1194410:intron 35.0%
ATCCTACTCTTCTTCTCTTT+CGG - chr8_1:41589918-41589937 None:intergenic 35.0%
GTTGTAACCTACTAGTACTA+GGG + chr8_1:41590181-41590200 Msa1194410:three_prime_UTR 35.0%
CAAAATTCCCTAGTACTAGT+AGG - chr8_1:41590191-41590210 None:intergenic 35.0%
!!! TATATGTTAGCTTTTGGGAG+GGG - chr8_1:41590237-41590256 None:intergenic 35.0%
GGACGAAGAAAATAAACGAG+AGG + chr8_1:41587488-41587507 Msa1194410:five_prime_UTR 40.0%
ATAAACGAGAGGCAATATGC+AGG + chr8_1:41587499-41587518 Msa1194410:exon 40.0%
CAAGCTAAATACGTTGGAAC+TGG + chr8_1:41587663-41587682 Msa1194410:CDS 40.0%
! ACAGCGTTACACTTTTATGC+AGG + chr8_1:41587809-41587828 Msa1194410:CDS 40.0%
ACAAGAAAATGCACTGCAAC+AGG - chr8_1:41588035-41588054 None:intergenic 40.0%
TTGTTGCTGCAAATAGGCTA+CGG + chr8_1:41588063-41588082 Msa1194410:intron 40.0%
AATGGTACACTTAGCTAGAG+AGG + chr8_1:41588117-41588136 Msa1194410:intron 40.0%
TCAGTTTAAGAAGGGAACAG+TGG + chr8_1:41588362-41588381 Msa1194410:intron 40.0%
CAGTTTAAGAAGGGAACAGT+GGG + chr8_1:41588363-41588382 Msa1194410:intron 40.0%
ATCTTCTGATTTCTGCTTGC+GGG + chr8_1:41588418-41588437 Msa1194410:intron 40.0%
ATGGACAAGAAAGGAAGGAA+AGG - chr8_1:41588684-41588703 None:intergenic 40.0%
TGAAACAGATGAGGGAAAAC+TGG - chr8_1:41588713-41588732 None:intergenic 40.0%
CAGAGAAGAACAGGAAGAAT+TGG - chr8_1:41588755-41588774 None:intergenic 40.0%
CAATGAAAGCAGAGAAGAAC+AGG - chr8_1:41588764-41588783 None:intergenic 40.0%
CTAAGAATGCTCCTGGAAAT+AGG + chr8_1:41588871-41588890 Msa1194410:intron 40.0%
ACACAATCACTCCTATTTCC+AGG - chr8_1:41588885-41588904 None:intergenic 40.0%
AAAAAGGGAAAGCAGTGAAG+TGG + chr8_1:41588917-41588936 Msa1194410:intron 40.0%
ACATCGTGTTCAATGGGATT+AGG - chr8_1:41589084-41589103 None:intergenic 40.0%
GATGTTATAAAGGATGAGGG+TGG + chr8_1:41589099-41589118 Msa1194410:intron 40.0%
! ATCGTCATCTCTTCTAGAAG+TGG - chr8_1:41589135-41589154 None:intergenic 40.0%
TACACAAAATTGGAAAGGGG+GGG - chr8_1:41589438-41589457 None:intergenic 40.0%
TGGTGACATCTCAATATCTG+AGG + chr8_1:41589539-41589558 Msa1194410:intron 40.0%
!!! ATTTTTTGTTAGTTGGGGGG+AGG + chr8_1:41589680-41589699 Msa1194410:intron 40.0%
!!! TTTTTTGTTAGTTGGGGGGA+GGG + chr8_1:41589681-41589700 Msa1194410:intron 40.0%
AATAAACCAAGGAGTGACAG+TGG - chr8_1:41589746-41589765 None:intergenic 40.0%
! CAGAAAATGCTTTTGCCTTG+TGG + chr8_1:41589884-41589903 Msa1194410:intron 40.0%
TCCTACTCTTCTTCTCTTTC+GGG - chr8_1:41589917-41589936 None:intergenic 40.0%
TGTACGGTACAATCTGATTG+AGG + chr8_1:41590002-41590021 Msa1194410:three_prime_UTR 40.0%
! GTTTTTATGGTATGGGGCTT+TGG + chr8_1:41590095-41590114 Msa1194410:three_prime_UTR 40.0%
TTCTACAACTCATTGTCTCG+TGG - chr8_1:41590129-41590148 None:intergenic 40.0%
GGTTGTAACCTACTAGTACT+AGG + chr8_1:41590180-41590199 Msa1194410:three_prime_UTR 40.0%
GTTTATAAAAGAGGGAGCGG+TGG + chr8_1:41587404-41587423 Msa1194410:five_prime_UTR 45.0%
CGTTGTCAGTAGTACTGCTA+TGG + chr8_1:41587965-41587984 Msa1194410:intron 45.0%
TGTTGCTGCAAATAGGCTAC+GGG + chr8_1:41588064-41588083 Msa1194410:intron 45.0%
TAAGAGAGAATTGGGGCCTT+CGG + chr8_1:41588195-41588214 Msa1194410:intron 45.0%
CAGCAAACTTTCTAACCCGA+AGG - chr8_1:41588214-41588233 None:intergenic 45.0%
GATTTGAACCTCAGAGAGAG+AGG + chr8_1:41588333-41588352 Msa1194410:intron 45.0%
GAAATACACCTCTCTCTCTG+AGG - chr8_1:41588344-41588363 None:intergenic 45.0%
! GGTTTTCTTTATGACCCGCA+TGG - chr8_1:41588493-41588512 None:intergenic 45.0%
AGACAAAAATGGTCGCAGCA+GGG - chr8_1:41588649-41588668 None:intergenic 45.0%
TAGACAAAAATGGTCGCAGC+AGG - chr8_1:41588650-41588669 None:intergenic 45.0%
CAGAGATGGACAAGAAAGGA+AGG - chr8_1:41588689-41588708 None:intergenic 45.0%
TGGACAGAGATGGACAAGAA+AGG - chr8_1:41588693-41588712 None:intergenic 45.0%
ACAGGAAGAATTGGACGTGA+GGG - chr8_1:41588746-41588765 None:intergenic 45.0%
AACAGGAAGAATTGGACGTG+AGG - chr8_1:41588747-41588766 None:intergenic 45.0%
TTCTCATGTTGCTACCAGTG+AGG + chr8_1:41588803-41588822 Msa1194410:intron 45.0%
TCTCATGTTGCTACCAGTGA+GGG + chr8_1:41588804-41588823 Msa1194410:intron 45.0%
AGATGACGATTAGGCGTTCT+TGG + chr8_1:41589145-41589164 Msa1194410:intron 45.0%
ACACAAAATTGGAAAGGGGG+GGG - chr8_1:41589437-41589456 None:intergenic 45.0%
! TTTTTGTTAGTTGGGGGGAG+GGG + chr8_1:41589682-41589701 Msa1194410:intron 45.0%
! TTTGGGGTTGGAGTGATAAG+GGG - chr8_1:41589723-41589742 None:intergenic 45.0%
! GTTTGGGGTTGGAGTGATAA+GGG - chr8_1:41589724-41589743 None:intergenic 45.0%
TACTCTTCTTCTCTTTCGGG+AGG - chr8_1:41589914-41589933 None:intergenic 45.0%
TCCCGAAAGAGAAGAAGAGT+AGG + chr8_1:41589913-41589932 Msa1194410:CDS 45.0%
TACCGTACAAGTACAACGAG+CGG - chr8_1:41589991-41590010 None:intergenic 45.0%
!! TAGCTTTTGGGAGGGGTATA+TGG - chr8_1:41590230-41590249 None:intergenic 45.0%
!!! TATTTTATTAGAAATAAATA+GGG - chr8_1:41588283-41588302 None:intergenic 5.0%
!!! TATTAATTATTTTAAAAACA+GGG + chr8_1:41588566-41588585 Msa1194410:intron 5.0%
!! TGAAATTAATTAATAATTTA+AGG - chr8_1:41588595-41588614 None:intergenic 5.0%
TAGCAAGACGCGAGTAGAGT+AGG + chr8_1:41587458-41587477 Msa1194410:five_prime_UTR 50.0%
AGCAAGACGCGAGTAGAGTA+GGG + chr8_1:41587459-41587478 Msa1194410:five_prime_UTR 50.0%
GCGAGTAGAGTAGGGTTTGA+GGG + chr8_1:41587467-41587486 Msa1194410:five_prime_UTR 50.0%
AGCAGTACTACTGACAACGC+AGG - chr8_1:41587964-41587983 None:intergenic 50.0%
GTCAGTAGTACTGCTATGGC+AGG + chr8_1:41587969-41587988 Msa1194410:intron 50.0%
ACTGCTATGGCAGGCAATAG+CGG + chr8_1:41587978-41587997 Msa1194410:intron 50.0%
! AGGCAATAGCGGAGAGGATT+AGG + chr8_1:41587989-41588008 Msa1194410:intron 50.0%
! GGCAATAGCGGAGAGGATTA+GGG + chr8_1:41587990-41588009 Msa1194410:intron 50.0%
! GCAATAGCGGAGAGGATTAG+GGG + chr8_1:41587991-41588010 Msa1194410:intron 50.0%
GTTGCTGCAAATAGGCTACG+GGG + chr8_1:41588065-41588084 Msa1194410:intron 50.0%
!! TACGGGGAGAGTGTTAGTTC+TGG + chr8_1:41588081-41588100 Msa1194410:intron 50.0%
AAGAGAGAATTGGGGCCTTC+GGG + chr8_1:41588196-41588215 Msa1194410:intron 50.0%
AAGAAGGGAACAGTGGGTCA+TGG + chr8_1:41588369-41588388 Msa1194410:intron 50.0%
TTCTCACCTGCGAGCAGAAA+CGG - chr8_1:41588456-41588475 None:intergenic 50.0%
GACAAAAATGGTCGCAGCAG+GGG - chr8_1:41588648-41588667 None:intergenic 50.0%
GAGGGAAAACTGGACAGAGA+TGG - chr8_1:41588703-41588722 None:intergenic 50.0%
AGTAGCAGCAAATCCCTCAC+TGG - chr8_1:41588820-41588839 None:intergenic 50.0%
AAGGGAAAGCAGTGAAGTGG+AGG + chr8_1:41588920-41588939 Msa1194410:intron 50.0%
AGGGAAAGCAGTGAAGTGGA+GGG + chr8_1:41588921-41588940 Msa1194410:intron 50.0%
ACAAAATTGGAAAGGGGGGG+GGG - chr8_1:41589435-41589454 None:intergenic 50.0%
CACAAAATTGGAAAGGGGGG+GGG - chr8_1:41589436-41589455 None:intergenic 50.0%
! TTTTGTTAGTTGGGGGGAGG+GGG + chr8_1:41589683-41589702 Msa1194410:intron 50.0%
GGAGTGATAAGGGGGTCATA+AGG - chr8_1:41589714-41589733 None:intergenic 50.0%
TTGGGGTTGGAGTGATAAGG+GGG - chr8_1:41589722-41589741 None:intergenic 50.0%
! GGTTTGGGGTTGGAGTGATA+AGG - chr8_1:41589725-41589744 None:intergenic 50.0%
CAAGGAGTGACAGTGGTTTG+GGG - chr8_1:41589739-41589758 None:intergenic 50.0%
CCAAGGAGTGACAGTGGTTT+GGG - chr8_1:41589740-41589759 None:intergenic 50.0%
ACCAAGGAGTGACAGTGGTT+TGG - chr8_1:41589741-41589760 None:intergenic 50.0%
GACCGCTCGTTGTACTTGTA+CGG + chr8_1:41589986-41590005 Msa1194410:three_prime_UTR 50.0%
CGCGAGTAGAGTAGGGTTTG+AGG + chr8_1:41587466-41587485 Msa1194410:five_prime_UTR 55.0%
! TATGGCAGGCAATAGCGGAG+AGG + chr8_1:41587983-41588002 Msa1194410:intron 55.0%
GAATACCCGTTTCTGCTCGC+AGG + chr8_1:41588447-41588466 Msa1194410:intron 55.0%
TCTCACCTGCGAGCAGAAAC+GGG - chr8_1:41588455-41588474 None:intergenic 55.0%
GTCGCAGCAGGGGATAAAGA+GGG - chr8_1:41588638-41588657 None:intergenic 55.0%
AAAATTGGAAAGGGGGGGGG+GGG - chr8_1:41589433-41589452 None:intergenic 55.0%
CAAAATTGGAAAGGGGGGGG+GGG - chr8_1:41589434-41589453 None:intergenic 55.0%
! GAGTGACAGTGGTTTGGGGT+TGG - chr8_1:41589735-41589754 None:intergenic 55.0%
CCCAAACCACTGTCACTCCT+TGG + chr8_1:41589737-41589756 Msa1194410:intron 55.0%
GGTCGCAGCAGGGGATAAAG+AGG - chr8_1:41588639-41588658 None:intergenic 60.0%
AAATTGGAAAGGGGGGGGGG+GGG - chr8_1:41589432-41589451 None:intergenic 60.0%
CTTTCGGGAGGCAGACCACA+AGG - chr8_1:41589902-41589921 None:intergenic 60.0%
ACGGCGTTGCGTTGCGTTGC+AGG + chr8_1:41587599-41587618 Msa1194410:intron 65.0%
AATTGGAAAGGGGGGGGGGG+GGG - chr8_1:41589431-41589450 None:intergenic 65.0%
ATTGGAAAGGGGGGGGGGGG+GGG - chr8_1:41589430-41589449 None:intergenic 70.0%
TTGGAAAGGGGGGGGGGGGG+GGG - chr8_1:41589429-41589448 None:intergenic 75.0%
! TGGAAAGGGGGGGGGGGGGG+GGG - chr8_1:41589428-41589447 None:intergenic 80.0%
! AAAGGGGGGGGGGGGGGGGG+GGG - chr8_1:41589425-41589444 None:intergenic 85.0%
! GAAAGGGGGGGGGGGGGGGG+GGG - chr8_1:41589426-41589445 None:intergenic 85.0%
! GGAAAGGGGGGGGGGGGGGG+GGG - chr8_1:41589427-41589446 None:intergenic 85.0%
! AAGGGGGGGGGGGGGGGGGG+GGG - chr8_1:41589424-41589443 None:intergenic 90.0%
! AGGGGGGGGGGGGGGGGGGG+GGG - chr8_1:41589423-41589442 None:intergenic 95.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 41587399 41590280 41587399 ID=Msa1194410;Name=Msa1194410
chr8_1 mRNA 41587399 41590280 41587399 ID=Msa1194410-mRNA-1;Parent=Msa1194410;Name=Msa1194410-mRNA-1;_AED=0.01;_eAED=0.01;_QI=116|1|1|1|0|0|3|346|87
chr8_1 exon 41587399 41587520 41587399 ID=Msa1194410-mRNA-1:exon:13452;Parent=Msa1194410-mRNA-1
chr8_1 exon 41587621 41587830 41587621 ID=Msa1194410-mRNA-1:exon:13453;Parent=Msa1194410-mRNA-1
chr8_1 exon 41589887 41590280 41589887 ID=Msa1194410-mRNA-1:exon:13454;Parent=Msa1194410-mRNA-1
chr8_1 five_prime_UTR 41587399 41587514 41587399 ID=Msa1194410-mRNA-1:five_prime_utr;Parent=Msa1194410-mRNA-1
chr8_1 CDS 41587515 41587520 41587515 ID=Msa1194410-mRNA-1:cds;Parent=Msa1194410-mRNA-1
chr8_1 CDS 41587621 41587830 41587621 ID=Msa1194410-mRNA-1:cds;Parent=Msa1194410-mRNA-1
chr8_1 CDS 41589887 41589934 41589887 ID=Msa1194410-mRNA-1:cds;Parent=Msa1194410-mRNA-1
chr8_1 three_prime_UTR 41589935 41590280 41589935 ID=Msa1194410-mRNA-1:three_prime_utr;Parent=Msa1194410-mRNA-1
Gene Sequence

>Msa1194410

ATGCAGGCGAGTGATAGATTTAACATAAATTCCCAACTTGAACATCTTCAAGCTAAATACGTTGGAACTGGACATGCTGATATCAACAGATTTGAATGGGCTGTGAATATTCAACGCGATAGCTATGCTTCATACATTGGCCATTACCCTTTACTTTCTTACTTTTCCATCGCTCAAAACGAATCTATTGGTAGACAGCGTTACACTTTTATGCAGAAAATGCTTTTGCCTTGTGGTCTGCCTCCCGAAAGAGAAGAAGAGTAG
Protein sequence

>Msa1194410

MQASDRFNINSQLEHLQAKYVGTGHADINRFEWAVNIQRDSYASYIGHYPLLSYFSIAQNESIGRQRYTFMQKMLLPCGLPPEREEE*