Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0071340 | Msa1196030 | 0.802342 | 5.870515e-49 | -8.615850e-47 |
| Msa0211680 | Msa1196030 | 0.807610 | 4.647918e-50 | -8.615850e-47 |
| Msa1186900 | Msa1196030 | 0.800903 | 1.158588e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 3 sgRNAs with CRISPR-Local
Find 5 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGCATTCCACGATCAAT+TGG | 0.442132 | 8_1:+44612754 | None:intergenic |
| TTATCATCAAACATTCTCAC+AGG | 0.584086 | 8_1:+44612805 | Msa1196030:CDS |
| GTGCAGTTGCCAATTGATCG+TGG | 0.662633 | 8_1:-44612763 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAAATTCAATAAAAAAAA+AGG | - | chr8_1:44612927-44612946 | None:intergenic | 10.0% |
| !!! | ATAATTATGAATCTTATTTC+AGG | - | chr8_1:44612861-44612880 | None:intergenic | 15.0% |
| TTATCATCAAACATTCTCAC+AGG | + | chr8_1:44612805-44612824 | Msa1196030:CDS | 30.0% | |
| ! | AAGGTAACTGATCAGCTTTT+AGG | - | chr8_1:44612908-44612927 | None:intergenic | 35.0% |
| GTGCAGTTGCCAATTGATCG+TGG | - | chr8_1:44612766-44612785 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 44612757 | 44613000 | 44612757 | ID=Msa1196030;Name=Msa1196030 |
| chr8_1 | mRNA | 44612757 | 44613000 | 44612757 | ID=Msa1196030-mRNA-1;Parent=Msa1196030;Name=Msa1196030-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|0|0|1|0|0|2|0|36 |
| chr8_1 | exon | 44612757 | 44612826 | 44612757 | ID=Msa1196030-mRNA-1:exon:14399;Parent=Msa1196030-mRNA-1 |
| chr8_1 | exon | 44612960 | 44613000 | 44612960 | ID=Msa1196030-mRNA-1:exon:14400;Parent=Msa1196030-mRNA-1 |
| chr8_1 | CDS | 44612757 | 44612826 | 44612757 | ID=Msa1196030-mRNA-1:cds;Parent=Msa1196030-mRNA-1 |
| chr8_1 | CDS | 44612960 | 44613000 | 44612960 | ID=Msa1196030-mRNA-1:cds;Parent=Msa1196030-mRNA-1 |
| Gene Sequence |
| Protein sequence |
