AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1197350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1197350 MtrunA17_Chr8g0366071 97.414 116 3 0 1 116 1 116 1.31e-74 226
Msa1197350 MtrunA17_Chr5g0420791 50.862 116 53 3 1 116 1 112 2.50e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 96 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGAAGCCTTAACTTTAA+TGG 0.078144 8_1:+47653466 Msa1197350:three_prime_UTR
TTGCTCCATTGGTGGGTTTA+TGG 0.104561 8_1:+47653009 Msa1197350:three_prime_UTR
GCCAAAACTATTAAAGTATT+TGG 0.136596 8_1:+47653035 Msa1197350:three_prime_UTR
TATGAAAGTGTGAACTTAAA+AGG 0.155654 8_1:-47653227 None:intergenic
ACCAAATACTTTAATAGTTT+TGG 0.194143 8_1:-47653036 None:intergenic
TGCTCCATTGGTGGGTTTAT+GGG 0.243123 8_1:+47653010 Msa1197350:three_prime_UTR
TCATTATCATGTATGGGATT+TGG 0.249198 8_1:-47653688 None:intergenic
GAACGGAGATTCAACCTTAA+TGG 0.264448 8_1:+47653488 Msa1197350:three_prime_UTR
CCTCCTCTGGGTGGCGTCTC+TGG 0.267365 8_1:-47652748 None:intergenic
GGCGCTCCTTTCGATCTTTC+CGG 0.292826 8_1:-47652790 None:intergenic
TCATTGGGCGACCATCTATA+AGG 0.306874 8_1:+47653088 Msa1197350:three_prime_UTR
AGCTGATTCTTTGACTCCAT+TGG 0.322748 8_1:+47650987 Msa1197350:CDS
ACCATTATCATTATCATGTA+TGG 0.326091 8_1:-47653695 None:intergenic
CCTGTCTACGTACTTCTTCC+TGG 0.342398 8_1:-47652857 None:intergenic
CCCATACATGATAATGATAA+TGG 0.356008 8_1:+47653694 Msa1197350:three_prime_UTR
TCTCCGTTCCATTAAAGTTA+AGG 0.379572 8_1:-47653474 None:intergenic
AACTCCTACAAGATTATCAT+TGG 0.385296 8_1:+47653072 Msa1197350:three_prime_UTR
GGAAAATTCTACATCACTAC+TGG 0.386822 8_1:+47653514 Msa1197350:three_prime_UTR
TTCAAGAAAGTGTACCCTAT+TGG 0.391313 8_1:+47650886 Msa1197350:CDS
AGATGGTTGATCTATGAGTA+TGG 0.394760 8_1:-47653589 None:intergenic
CTTTGACTCCATTGGATGAA+AGG 0.411007 8_1:+47650995 Msa1197350:CDS
CACAAATAACATGGATGGAT+GGG 0.416845 8_1:+47653633 Msa1197350:three_prime_UTR
ACCCAACGTCTTTGTATACT+AGG 0.421732 8_1:+47653363 Msa1197350:three_prime_UTR
GGATGTGAAAAGAGTCCTAA+TGG 0.423619 8_1:+47652932 Msa1197350:three_prime_UTR
GATCTATGAGTATGGATTGT+GGG 0.426498 8_1:-47653581 None:intergenic
CTGAACTTCCTCGATCCATT+AGG 0.433414 8_1:-47652947 None:intergenic
AAAATTAGGTCTTGCTCCAT+TGG 0.434997 8_1:+47652998 Msa1197350:three_prime_UTR
AAATGTAAGAAAACAAGCTT+TGG 0.439042 8_1:+47650798 Msa1197350:CDS
CCATTAAAGTTAAGGCTTCA+AGG 0.445746 8_1:-47653466 None:intergenic
GCAAAACCGTATTGAGGGTA+TGG 0.465436 8_1:-47652811 None:intergenic
ATGATAATCTTGTAGGAGTT+GGG 0.467270 8_1:-47653069 None:intergenic
AATGATAATCTTGTAGGAGT+TGG 0.470398 8_1:-47653070 None:intergenic
CATTGGGCGACCATCTATAA+GGG 0.473480 8_1:+47653089 Msa1197350:three_prime_UTR
TCGCCCAATGATAATCTTGT+AGG 0.473637 8_1:-47653076 None:intergenic
ATGTGAAGACGATGGGGTCA+TGG 0.475582 8_1:-47652900 None:intergenic
CTTTACTTTACCCTTATAGA+TGG 0.477092 8_1:-47653099 None:intergenic
TCCTAGTATACAAAGACGTT+GGG 0.478824 8_1:-47653364 None:intergenic
TGTGAAGACGATGGGGTCAT+GGG 0.487600 8_1:-47652899 None:intergenic
TTCTTAGGAGTGAGATGTAG+TGG 0.487699 8_1:+47653402 Msa1197350:three_prime_UTR
ACACAAATAACATGGATGGA+TGG 0.505656 8_1:+47653632 Msa1197350:three_prime_UTR
CCATTATCATTATCATGTAT+GGG 0.508389 8_1:-47653694 None:intergenic
ATCCAGCAAAACCGTATTGA+GGG 0.515055 8_1:-47652816 None:intergenic
ATTAGGTCTTGCTCCATTGG+TGG 0.515733 8_1:+47653001 Msa1197350:three_prime_UTR
CATCTATAAGGGTAAAGTAA+AGG 0.518416 8_1:+47653100 Msa1197350:three_prime_UTR
TACTACACAAATAACATGGA+TGG 0.520051 8_1:+47653628 Msa1197350:three_prime_UTR
GTAGTGGGAACAAGCAAGAT+GGG 0.521103 8_1:+47653418 Msa1197350:three_prime_UTR
TTTCAGCTTCTTCACATGAA+AGG 0.522846 8_1:+47651040 Msa1197350:CDS
AAAATTCTAAAAGGAAGGAA+AGG 0.523431 8_1:+47653268 Msa1197350:three_prime_UTR
TGTAGTGGGAACAAGCAAGA+TGG 0.524946 8_1:+47653417 Msa1197350:three_prime_UTR
GAGATTCAACCTTAATGGAA+TGG 0.525649 8_1:+47653493 Msa1197350:three_prime_UTR
CTGAGCCAGGGGAATTGAAA+AGG 0.526681 8_1:+47651115 Msa1197350:CDS
CCACTAGTGAGTACTGAGCC+AGG 0.527168 8_1:+47651102 Msa1197350:CDS
CTTGAAAATGTGAAGACGAT+GGG 0.529891 8_1:-47652907 None:intergenic
ATCTATAAATAAAAGAATGT+TGG 0.532382 8_1:+47653550 Msa1197350:three_prime_UTR
CATCCAGCAAAACCGTATTG+AGG 0.534769 8_1:-47652817 None:intergenic
AAGCCTTAACTTTAATGGAA+CGG 0.535485 8_1:+47653471 Msa1197350:three_prime_UTR
TTAGGTCTTGCTCCATTGGT+GGG 0.542641 8_1:+47653002 Msa1197350:three_prime_UTR
CCAGGAAGAAGTACGTAGAC+AGG 0.545614 8_1:+47652857 Msa1197350:exon
TGATCTATGAGTATGGATTG+TGG 0.546028 8_1:-47653582 None:intergenic
ATCCTAGTATACAAAGACGT+TGG 0.547666 8_1:-47653365 None:intergenic
AGGGTAAAGTAAAGGAACCT+TGG 0.553505 8_1:+47653108 Msa1197350:three_prime_UTR
ACTCCTACAAGATTATCATT+GGG 0.555407 8_1:+47653073 Msa1197350:three_prime_UTR
GATGAAATCCTTTCATCCAA+TGG 0.555797 8_1:-47651003 None:intergenic
AAAAGAACGAGGACAGTCGC+CGG 0.559589 8_1:+47652771 Msa1197350:CDS
TCTTAGGAGTGAGATGTAGT+GGG 0.562756 8_1:+47653403 Msa1197350:three_prime_UTR
CCTGGCTCAGTACTCACTAG+TGG 0.563648 8_1:-47651102 None:intergenic
AGACAGGTGAGGTGAGGGCG+TGG 0.565623 8_1:+47652873 Msa1197350:exon
ATCTATGAGTATGGATTGTG+GGG 0.570948 8_1:-47653580 None:intergenic
CTATTGAACTCTTCATGCCA+AGG 0.572139 8_1:-47653125 None:intergenic
ACAAATAACATGGATGGATG+GGG 0.575133 8_1:+47653634 Msa1197350:three_prime_UTR
GGGGCGAGTCTAGTGCGTCC+AGG 0.580834 8_1:+47652839 Msa1197350:CDS
TACGTAGACAGGTGAGGTGA+GGG 0.584541 8_1:+47652868 Msa1197350:exon
AACGTCTTTGTATACTAGGA+TGG 0.586509 8_1:+47653367 Msa1197350:three_prime_UTR
GTACGTAGACAGGTGAGGTG+AGG 0.588117 8_1:+47652867 Msa1197350:exon
CACTAGTGAGTACTGAGCCA+GGG 0.593056 8_1:+47651103 Msa1197350:CDS
AAATTACTACACAAATAACA+TGG 0.599432 8_1:+47653624 Msa1197350:three_prime_UTR
TCAAGGTCAATTCCCATGTG+TGG 0.607645 8_1:-47653449 None:intergenic
TTCATGTGTTGATAGGTGAA+AGG 0.610044 8_1:+47652710 Msa1197350:intron
GAAAATTCTACATCACTACT+GGG 0.611516 8_1:+47653515 Msa1197350:three_prime_UTR
CAGTCGCCGGAAAGATCGAA+AGG 0.611721 8_1:+47652784 Msa1197350:CDS
GCATCCCATAAACCCACCAA+TGG 0.613697 8_1:-47653014 None:intergenic
CCAGAGACGCCACCCAGAGG+AGG 0.621794 8_1:+47652748 Msa1197350:CDS
GTGAAGACGATGGGGTCATG+GGG 0.624372 8_1:-47652898 None:intergenic
ATGGGGAAATATTCCACACA+TGG 0.624415 8_1:+47653436 Msa1197350:three_prime_UTR
CCCAGAGGAGGAAAAGAACG+AGG 0.627011 8_1:+47652760 Msa1197350:CDS
TAAGAAAACAAGCTTTGGAG+AGG 0.633132 8_1:+47650803 Msa1197350:CDS
ACTTGAAAATGTGAAGACGA+TGG 0.647764 8_1:-47652908 None:intergenic
AAGAAGTACGTAGACAGGTG+AGG 0.649670 8_1:+47652862 Msa1197350:exon
AAAAGAGTCCTAATGGATCG+AGG 0.654847 8_1:+47652939 Msa1197350:three_prime_UTR
TGATAGGTGAAAGGAGAACG+AGG 0.657845 8_1:+47652719 Msa1197350:intron
ACTAGTGAGTACTGAGCCAG+GGG 0.660813 8_1:+47651104 Msa1197350:CDS
ATGGATGGGGAAGAGATCAA+AGG 0.660831 8_1:+47653647 Msa1197350:three_prime_UTR
TTGAAAATGTGAAGACGATG+GGG 0.668931 8_1:-47652906 None:intergenic
TGGGGAAATATTCCACACAT+GGG 0.669407 8_1:+47653437 Msa1197350:three_prime_UTR
TAGTGGGAACAAGCAAGATG+GGG 0.676322 8_1:+47653419 Msa1197350:three_prime_UTR
TTAAAAGGTATGCAAATGAG+CGG 0.678682 8_1:-47653212 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTGAAAATAAACATTT+CGG + chr8_1:47652298-47652317 Msa1197350:intron 10.0%
!!! TACTTATTTTTATATTTTGA+GGG - chr8_1:47653765-47653784 None:intergenic 10.0%
!!! TTTTATTATGGAAGAAATTT+TGG - chr8_1:47650864-47650883 None:intergenic 15.0%
!!! GTTTTTTTCTTATACATTAT+TGG + chr8_1:47651262-47651281 Msa1197350:intron 15.0%
!! TTAAATTTCTTAATGTTGTA+TGG - chr8_1:47651487-47651506 None:intergenic 15.0%
!!! TATTGAAATTATTTTAGCAA+TGG - chr8_1:47651523-47651542 None:intergenic 15.0%
!!! AAATAATTTAAAACTGAAAG+TGG - chr8_1:47651996-47652015 None:intergenic 15.0%
!! AAATATAATCACAATTAAGA+TGG + chr8_1:47652123-47652142 Msa1197350:intron 15.0%
!!! GTATGTATATGTTTTTTTTT+TGG - chr8_1:47653193-47653212 None:intergenic 15.0%
!!! CATTCTTTTATTTATAGATA+AGG - chr8_1:47653549-47653568 None:intergenic 15.0%
!! ATCTATAAATAAAAGAATGT+TGG + chr8_1:47653550-47653569 Msa1197350:three_prime_UTR 15.0%
!!! GTACTTATTTTTATATTTTG+AGG - chr8_1:47653766-47653785 None:intergenic 15.0%
!! ATCTTCAAAAACTATTTAAA+AGG + chr8_1:47653820-47653839 Msa1197350:three_prime_UTR 15.0%
!!! TTTGTAGCTATTATTTTTTA+AGG + chr8_1:47653852-47653871 Msa1197350:three_prime_UTR 15.0%
!!! CTTTCTTGAAGATTTTATTA+TGG - chr8_1:47650876-47650895 None:intergenic 20.0%
!! TATAATGAAATGATATTGCA+TGG - chr8_1:47651185-47651204 None:intergenic 20.0%
!!! TTAGAGTTTTAATACTAAGT+AGG + chr8_1:47651438-47651457 Msa1197350:intron 20.0%
!! AAATAAACATTTCGGTTAAA+TGG + chr8_1:47652306-47652325 Msa1197350:intron 20.0%
!! AGGATAATTAATATAGTTGT+TGG + chr8_1:47652420-47652439 Msa1197350:intron 20.0%
!! ATAATTAATATAGTTGTTGG+TGG + chr8_1:47652423-47652442 Msa1197350:intron 20.0%
!!! ACCAAATACTTTAATAGTTT+TGG - chr8_1:47653039-47653058 None:intergenic 20.0%
!! ACTTTCATAAAAATATTGCA+TGG + chr8_1:47653241-47653260 Msa1197350:three_prime_UTR 20.0%
!!! TGGAAAGTATTAATTTTCTT+AGG + chr8_1:47653387-47653406 Msa1197350:three_prime_UTR 20.0%
!! AATTTCAAAGTTGAAAAAGA+TGG - chr8_1:47653609-47653628 None:intergenic 20.0%
!! AAATTACTACACAAATAACA+TGG + chr8_1:47653624-47653643 Msa1197350:three_prime_UTR 20.0%
!! TCAAAAACTATTTAAAAGGA+AGG + chr8_1:47653824-47653843 Msa1197350:three_prime_UTR 20.0%
! AAATGTAAGAAAACAAGCTT+TGG + chr8_1:47650798-47650817 Msa1197350:CDS 25.0%
! GTCAAATATGTATCATATTG+CGG - chr8_1:47651385-47651404 None:intergenic 25.0%
! TTGATATTGCGAAAAAATTG+CGG - chr8_1:47651407-47651426 None:intergenic 25.0%
! TATGGTAAAGTATATGACAA+TGG - chr8_1:47651469-47651488 None:intergenic 25.0%
! AGCTCAAAACAACTAATTAA+TGG - chr8_1:47651587-47651606 None:intergenic 25.0%
! AGTACAAATTAATGCTAAGT+TGG - chr8_1:47651733-47651752 None:intergenic 25.0%
! TCCTATTTATAGGAGAAATT+TGG + chr8_1:47651761-47651780 Msa1197350:intron 25.0%
! CCTATTTATAGGAGAAATTT+GGG + chr8_1:47651762-47651781 Msa1197350:intron 25.0%
! TCAACAAAAGTGAATGTATT+TGG - chr8_1:47651824-47651843 None:intergenic 25.0%
! CCTATTTATAAGAGAAAGTT+AGG - chr8_1:47651847-47651866 None:intergenic 25.0%
! CCTAACTTTCTCTTATAAAT+AGG + chr8_1:47651844-47651863 Msa1197350:intron 25.0%
!! AATTTAAAACTGAAAGTGGT+AGG - chr8_1:47651992-47652011 None:intergenic 25.0%
! CAGTGTTTCGAAAAAATTTA+TGG + chr8_1:47652068-47652087 Msa1197350:intron 25.0%
! GACTTGAGTAATTTATTCTT+GGG - chr8_1:47652172-47652191 None:intergenic 25.0%
! TGACTTGAGTAATTTATTCT+TGG - chr8_1:47652173-47652192 None:intergenic 25.0%
!! CGATTTGATAATCATTAGTT+TGG - chr8_1:47652206-47652225 None:intergenic 25.0%
!! GCCAAAACTATTAAAGTATT+TGG + chr8_1:47653035-47653054 Msa1197350:three_prime_UTR 25.0%
!!! GTTTTTTTTTTGGTACAAAG+CGG - chr8_1:47653183-47653202 None:intergenic 25.0%
!! TATGAAAGTGTGAACTTAAA+AGG - chr8_1:47653230-47653249 None:intergenic 25.0%
! CATGGAAGAAAAATTCTAAA+AGG + chr8_1:47653259-47653278 Msa1197350:three_prime_UTR 25.0%
!! GAAGAAAAATTCTAAAAGGA+AGG + chr8_1:47653263-47653282 Msa1197350:three_prime_UTR 25.0%
!! AAAATTCTAAAAGGAAGGAA+AGG + chr8_1:47653268-47653287 Msa1197350:three_prime_UTR 25.0%
!! TAGAATTTTCCATTCCATTA+AGG - chr8_1:47653505-47653524 None:intergenic 25.0%
! CCATTATCATTATCATGTAT+GGG - chr8_1:47653697-47653716 None:intergenic 25.0%
! ACCATTATCATTATCATGTA+TGG - chr8_1:47653698-47653717 None:intergenic 25.0%
!!! AGCTATTATTTTTTAAGGAG+CGG + chr8_1:47653857-47653876 Msa1197350:three_prime_UTR 25.0%
!!! TGCTTTTTTGAAGTCCAATA+GGG - chr8_1:47650903-47650922 None:intergenic 30.0%
AACTGGATCACAAAAAATAG+TGG + chr8_1:47651141-47651160 Msa1197350:intron 30.0%
CATTAAGATTGAATATGCCA+CGG + chr8_1:47651285-47651304 Msa1197350:intron 30.0%
!!! GCAATTTTTTCGCAATATCA+AGG + chr8_1:47651406-47651425 Msa1197350:intron 30.0%
ATTTCTCCTATAAATAGGAC+CGG - chr8_1:47651760-47651779 None:intergenic 30.0%
CCCAAATTTCTCCTATAAAT+AGG - chr8_1:47651765-47651784 None:intergenic 30.0%
CTTTCTCTTATAAATAGGAC+CGG + chr8_1:47651849-47651868 Msa1197350:intron 30.0%
GCAAAATTTAATACTCTCTC+CGG - chr8_1:47651871-47651890 None:intergenic 30.0%
! ATTTTGCAATACCAACATAG+TGG + chr8_1:47651884-47651903 Msa1197350:intron 30.0%
ATTAAGTGTAACCACTATGT+TGG - chr8_1:47651898-47651917 None:intergenic 30.0%
AACTGATAATAGCTAGTTGT+AGG - chr8_1:47652032-47652051 None:intergenic 30.0%
CGAGTTATATACCTAGTTTA+TGG - chr8_1:47652241-47652260 None:intergenic 30.0%
!!! AGTGTTTTTGCTTTTTCTCT+TGG + chr8_1:47652542-47652561 Msa1197350:intron 30.0%
! AATCCAATTCATGTGTTGAT+AGG + chr8_1:47652703-47652722 Msa1197350:intron 30.0%
TAAGAAGCTTCCGAAAAATT+AGG + chr8_1:47652984-47653003 Msa1197350:three_prime_UTR 30.0%
ATGATAATCTTGTAGGAGTT+GGG - chr8_1:47653072-47653091 None:intergenic 30.0%
AATGATAATCTTGTAGGAGT+TGG - chr8_1:47653073-47653092 None:intergenic 30.0%
AACTCCTACAAGATTATCAT+TGG + chr8_1:47653072-47653091 Msa1197350:three_prime_UTR 30.0%
ACTCCTACAAGATTATCATT+GGG + chr8_1:47653073-47653092 Msa1197350:three_prime_UTR 30.0%
CTTTACTTTACCCTTATAGA+TGG - chr8_1:47653102-47653121 None:intergenic 30.0%
CATCTATAAGGGTAAAGTAA+AGG + chr8_1:47653100-47653119 Msa1197350:three_prime_UTR 30.0%
! TTAAAAGGTATGCAAATGAG+CGG - chr8_1:47653215-47653234 None:intergenic 30.0%
!!! ATTTTTTAAGTGCACAAGTG+AGG + chr8_1:47653307-47653326 Msa1197350:three_prime_UTR 30.0%
!!! TTTTTTAAGTGCACAAGTGA+GGG + chr8_1:47653308-47653327 Msa1197350:three_prime_UTR 30.0%
AAGCCTTAACTTTAATGGAA+CGG + chr8_1:47653471-47653490 Msa1197350:three_prime_UTR 30.0%
GAAAATTCTACATCACTACT+GGG + chr8_1:47653515-47653534 Msa1197350:three_prime_UTR 30.0%
TACTACACAAATAACATGGA+TGG + chr8_1:47653628-47653647 Msa1197350:three_prime_UTR 30.0%
TCATTATCATGTATGGGATT+TGG - chr8_1:47653691-47653710 None:intergenic 30.0%
! CCCATACATGATAATGATAA+TGG + chr8_1:47653694-47653713 Msa1197350:three_prime_UTR 30.0%
!! TAAGAAAACAAGCTTTGGAG+AGG + chr8_1:47650803-47650822 Msa1197350:CDS 35.0%
! TTCAAGAAAGTGTACCCTAT+TGG + chr8_1:47650886-47650905 Msa1197350:CDS 35.0%
!!! GTGCTTTTTTGAAGTCCAAT+AGG - chr8_1:47650904-47650923 None:intergenic 35.0%
GATGAAATCCTTTCATCCAA+TGG - chr8_1:47651006-47651025 None:intergenic 35.0%
TTTCAGCTTCTTCACATGAA+AGG + chr8_1:47651040-47651059 Msa1197350:CDS 35.0%
!!! TATGCCACGGTTTTAGAAAA+TGG + chr8_1:47651298-47651317 Msa1197350:intron 35.0%
!!! CATACCATTTTCTAAAACCG+TGG - chr8_1:47651305-47651324 None:intergenic 35.0%
TGGTATGTGATAAGTGTGTT+AGG + chr8_1:47651318-47651337 Msa1197350:intron 35.0%
GGTATGTGATAAGTGTGTTA+GGG + chr8_1:47651319-47651338 Msa1197350:intron 35.0%
CTGCTTCACATCATTTCTAT+TGG + chr8_1:47651605-47651624 Msa1197350:intron 35.0%
CTGTGCACAAAAAAATCTGA+AGG + chr8_1:47651655-47651674 Msa1197350:intron 35.0%
!! GCATTAATTTGTACTTCCTC+CGG + chr8_1:47651738-47651757 Msa1197350:intron 35.0%
AGATGGCATTAATCTCAATC+AGG + chr8_1:47652140-47652159 Msa1197350:intron 35.0%
!! CGCTTTGTTTACCATAAACT+AGG + chr8_1:47652227-47652246 Msa1197350:intron 35.0%
! TTCTTTTCAATCAGAGCTAG+AGG + chr8_1:47652574-47652593 Msa1197350:CDS 35.0%
! TTTTTGCGAGCAATATTGAC+AGG - chr8_1:47652611-47652630 None:intergenic 35.0%
ACTGAAATAAGCAGTCAGAA+TGG + chr8_1:47652633-47652652 Msa1197350:intron 35.0%
CTGAAATAAGCAGTCAGAAT+GGG + chr8_1:47652634-47652653 Msa1197350:intron 35.0%
TCACCTATCAACACATGAAT+TGG - chr8_1:47652709-47652728 None:intergenic 35.0%
! TTCATGTGTTGATAGGTGAA+AGG + chr8_1:47652710-47652729 Msa1197350:intron 35.0%
TTGAAAATGTGAAGACGATG+GGG - chr8_1:47652909-47652928 None:intergenic 35.0%
CTTGAAAATGTGAAGACGAT+GGG - chr8_1:47652910-47652929 None:intergenic 35.0%
ACTTGAAAATGTGAAGACGA+TGG - chr8_1:47652911-47652930 None:intergenic 35.0%
!! ATCGTCTTCACATTTTCAAG+TGG + chr8_1:47652910-47652929 Msa1197350:three_prime_UTR 35.0%
!!! TCGTCTTCACATTTTCAAGT+GGG + chr8_1:47652911-47652930 Msa1197350:three_prime_UTR 35.0%
! TGGAGCAAGACCTAATTTTT+CGG - chr8_1:47652997-47653016 None:intergenic 35.0%
AAAATTAGGTCTTGCTCCAT+TGG + chr8_1:47652998-47653017 Msa1197350:three_prime_UTR 35.0%
TCCTAGTATACAAAGACGTT+GGG - chr8_1:47653367-47653386 None:intergenic 35.0%
ATCCTAGTATACAAAGACGT+TGG - chr8_1:47653368-47653387 None:intergenic 35.0%
AACGTCTTTGTATACTAGGA+TGG + chr8_1:47653367-47653386 Msa1197350:three_prime_UTR 35.0%
CCATTAAAGTTAAGGCTTCA+AGG - chr8_1:47653469-47653488 None:intergenic 35.0%
CCTTGAAGCCTTAACTTTAA+TGG + chr8_1:47653466-47653485 Msa1197350:three_prime_UTR 35.0%
TCTCCGTTCCATTAAAGTTA+AGG - chr8_1:47653477-47653496 None:intergenic 35.0%
GAGATTCAACCTTAATGGAA+TGG + chr8_1:47653493-47653512 Msa1197350:three_prime_UTR 35.0%
GGAAAATTCTACATCACTAC+TGG + chr8_1:47653514-47653533 Msa1197350:three_prime_UTR 35.0%
! ATCTATGAGTATGGATTGTG+GGG - chr8_1:47653583-47653602 None:intergenic 35.0%
! GATCTATGAGTATGGATTGT+GGG - chr8_1:47653584-47653603 None:intergenic 35.0%
! TGATCTATGAGTATGGATTG+TGG - chr8_1:47653585-47653604 None:intergenic 35.0%
AGATGGTTGATCTATGAGTA+TGG - chr8_1:47653592-47653611 None:intergenic 35.0%
ACACAAATAACATGGATGGA+TGG + chr8_1:47653632-47653651 Msa1197350:three_prime_UTR 35.0%
CACAAATAACATGGATGGAT+GGG + chr8_1:47653633-47653652 Msa1197350:three_prime_UTR 35.0%
ACAAATAACATGGATGGATG+GGG + chr8_1:47653634-47653653 Msa1197350:three_prime_UTR 35.0%
! AGCTGATTCTTTGACTCCAT+TGG + chr8_1:47650987-47651006 Msa1197350:CDS 40.0%
CTTTGACTCCATTGGATGAA+AGG + chr8_1:47650995-47651014 Msa1197350:CDS 40.0%
TATATAACTCGTGCGTTGCA+CGG + chr8_1:47652250-47652269 Msa1197350:intron 40.0%
ATCCAGCAAAACCGTATTGA+GGG - chr8_1:47652819-47652838 None:intergenic 40.0%
! TCAATACGGTTTTGCTGGAT+GGG + chr8_1:47652819-47652838 Msa1197350:CDS 40.0%
GGATGTGAAAAGAGTCCTAA+TGG + chr8_1:47652932-47652951 Msa1197350:three_prime_UTR 40.0%
! AAAAGAGTCCTAATGGATCG+AGG + chr8_1:47652939-47652958 Msa1197350:three_prime_UTR 40.0%
TCGCCCAATGATAATCTTGT+AGG - chr8_1:47653079-47653098 None:intergenic 40.0%
AGGGTAAAGTAAAGGAACCT+TGG + chr8_1:47653108-47653127 Msa1197350:three_prime_UTR 40.0%
CTATTGAACTCTTCATGCCA+AGG - chr8_1:47653128-47653147 None:intergenic 40.0%
! GGGTGAGATCCAAACTTTTT+CGG + chr8_1:47653328-47653347 Msa1197350:three_prime_UTR 40.0%
ACCCAACGTCTTTGTATACT+AGG + chr8_1:47653363-47653382 Msa1197350:three_prime_UTR 40.0%
TTCTTAGGAGTGAGATGTAG+TGG + chr8_1:47653402-47653421 Msa1197350:three_prime_UTR 40.0%
TCTTAGGAGTGAGATGTAGT+GGG + chr8_1:47653403-47653422 Msa1197350:three_prime_UTR 40.0%
ATGGGGAAATATTCCACACA+TGG + chr8_1:47653436-47653455 Msa1197350:three_prime_UTR 40.0%
TGGGGAAATATTCCACACAT+GGG + chr8_1:47653437-47653456 Msa1197350:three_prime_UTR 40.0%
GAACGGAGATTCAACCTTAA+TGG + chr8_1:47653488-47653507 Msa1197350:three_prime_UTR 40.0%
!!! AGGAGCGGCTCATTTTTTAA+TGG + chr8_1:47653872-47653891 Msa1197350:three_prime_UTR 40.0%
!!! GGAGCGGCTCATTTTTTAAT+GGG + chr8_1:47653873-47653892 Msa1197350:three_prime_UTR 40.0%
! TTGCACCTTTTCAATTCCCC+TGG - chr8_1:47651123-47651142 None:intergenic 45.0%
! GGGAATTGAAAAGGTGCAAC+TGG + chr8_1:47651124-47651143 Msa1197350:intron 45.0%
CTTCCTCCGGTCCTATTTAT+AGG + chr8_1:47651751-47651770 Msa1197350:intron 45.0%
TCTCCTATAAATAGGACCGG+AGG - chr8_1:47651757-47651776 None:intergenic 45.0%
GCTAGTTGTAGGTAAGCACA+AGG - chr8_1:47652021-47652040 None:intergenic 45.0%
ATATAACTCGTGCGTTGCAC+GGG + chr8_1:47652251-47652270 Msa1197350:intron 45.0%
TGATAGGTGAAAGGAGAACG+AGG + chr8_1:47652719-47652738 Msa1197350:intron 45.0%
GCAAAACCGTATTGAGGGTA+TGG - chr8_1:47652814-47652833 None:intergenic 45.0%
! TACCCTCAATACGGTTTTGC+TGG + chr8_1:47652814-47652833 Msa1197350:CDS 45.0%
CATCCAGCAAAACCGTATTG+AGG - chr8_1:47652820-47652839 None:intergenic 45.0%
! CTCAATACGGTTTTGCTGGA+TGG + chr8_1:47652818-47652837 Msa1197350:CDS 45.0%
! CAATACGGTTTTGCTGGATG+GGG + chr8_1:47652820-47652839 Msa1197350:CDS 45.0%
! AAGAAGTACGTAGACAGGTG+AGG + chr8_1:47652862-47652881 Msa1197350:exon 45.0%
CTGAACTTCCTCGATCCATT+AGG - chr8_1:47652950-47652969 None:intergenic 45.0%
ATTAGGTCTTGCTCCATTGG+TGG + chr8_1:47653001-47653020 Msa1197350:three_prime_UTR 45.0%
TTAGGTCTTGCTCCATTGGT+GGG + chr8_1:47653002-47653021 Msa1197350:three_prime_UTR 45.0%
!! TTGCTCCATTGGTGGGTTTA+TGG + chr8_1:47653009-47653028 Msa1197350:three_prime_UTR 45.0%
!! TGCTCCATTGGTGGGTTTAT+GGG + chr8_1:47653010-47653029 Msa1197350:three_prime_UTR 45.0%
TCATTGGGCGACCATCTATA+AGG + chr8_1:47653088-47653107 Msa1197350:three_prime_UTR 45.0%
CATTGGGCGACCATCTATAA+GGG + chr8_1:47653089-47653108 Msa1197350:three_prime_UTR 45.0%
!!! GATCCAAACTTTTTCGGACG+AGG + chr8_1:47653334-47653353 Msa1197350:three_prime_UTR 45.0%
! TGTAGTGGGAACAAGCAAGA+TGG + chr8_1:47653417-47653436 Msa1197350:three_prime_UTR 45.0%
! GTAGTGGGAACAAGCAAGAT+GGG + chr8_1:47653418-47653437 Msa1197350:three_prime_UTR 45.0%
TAGTGGGAACAAGCAAGATG+GGG + chr8_1:47653419-47653438 Msa1197350:three_prime_UTR 45.0%
TCAAGGTCAATTCCCATGTG+TGG - chr8_1:47653452-47653471 None:intergenic 45.0%
ATGGATGGGGAAGAGATCAA+AGG + chr8_1:47653647-47653666 Msa1197350:three_prime_UTR 45.0%
!!! GCGGCTCATTTTTTAATGGG+TGG + chr8_1:47653876-47653895 Msa1197350:three_prime_UTR 45.0%
!! TAAATTATATTAAATATAAG+AGG + chr8_1:47652351-47652370 Msa1197350:intron 5.0%
!! AAATTATATTAAATATAAGA+GGG + chr8_1:47652352-47652371 Msa1197350:intron 5.0%
!!! ATATTTGTTAAAATATTTTT+AGG + chr8_1:47652400-47652419 Msa1197350:intron 5.0%
!! CACTAGTGAGTACTGAGCCA+GGG + chr8_1:47651103-47651122 Msa1197350:CDS 50.0%
!! ACTAGTGAGTACTGAGCCAG+GGG + chr8_1:47651104-47651123 Msa1197350:CDS 50.0%
CTGAGCCAGGGGAATTGAAA+AGG + chr8_1:47651115-47651134 Msa1197350:CDS 50.0%
! CCTCGTTCTTTTCCTCCTCT+GGG - chr8_1:47652763-47652782 None:intergenic 50.0%
! TCCTCGTTCTTTTCCTCCTC+TGG - chr8_1:47652764-47652783 None:intergenic 50.0%
AAAAGAACGAGGACAGTCGC+CGG + chr8_1:47652771-47652790 Msa1197350:CDS 50.0%
CCTGTCTACGTACTTCTTCC+TGG - chr8_1:47652860-47652879 None:intergenic 50.0%
! CCAGGAAGAAGTACGTAGAC+AGG + chr8_1:47652857-47652876 Msa1197350:exon 50.0%
TACGTAGACAGGTGAGGTGA+GGG + chr8_1:47652868-47652887 Msa1197350:exon 50.0%
TGTGAAGACGATGGGGTCAT+GGG - chr8_1:47652902-47652921 None:intergenic 50.0%
ATGTGAAGACGATGGGGTCA+TGG - chr8_1:47652903-47652922 None:intergenic 50.0%
GCATCCCATAAACCCACCAA+TGG - chr8_1:47653017-47653036 None:intergenic 50.0%
GCACCTCGTCCGAAAAAGTT+TGG - chr8_1:47653340-47653359 None:intergenic 50.0%
CCTGGCTCAGTACTCACTAG+TGG - chr8_1:47651105-47651124 None:intergenic 55.0%
!! CCACTAGTGAGTACTGAGCC+AGG + chr8_1:47651102-47651121 Msa1197350:CDS 55.0%
! CGTTCTTTTCCTCCTCTGGG+TGG - chr8_1:47652760-47652779 None:intergenic 55.0%
CCCAGAGGAGGAAAAGAACG+AGG + chr8_1:47652760-47652779 Msa1197350:CDS 55.0%
CAGTCGCCGGAAAGATCGAA+AGG + chr8_1:47652784-47652803 Msa1197350:CDS 55.0%
GGCGCTCCTTTCGATCTTTC+CGG - chr8_1:47652793-47652812 None:intergenic 55.0%
! GGAGCGCCATACCCTCAATA+CGG + chr8_1:47652805-47652824 Msa1197350:CDS 55.0%
GTACGTAGACAGGTGAGGTG+AGG + chr8_1:47652867-47652886 Msa1197350:exon 55.0%
GTGAAGACGATGGGGTCATG+GGG - chr8_1:47652901-47652920 None:intergenic 55.0%
TGACCAGAGACGCCACCCAG+AGG + chr8_1:47652745-47652764 Msa1197350:CDS 65.0%
AGACAGGTGAGGTGAGGGCG+TGG + chr8_1:47652873-47652892 Msa1197350:exon 65.0%
CCTCCTCTGGGTGGCGTCTC+TGG - chr8_1:47652751-47652770 None:intergenic 70.0%
CCAGAGACGCCACCCAGAGG+AGG + chr8_1:47652748-47652767 Msa1197350:CDS 70.0%
GGGGCGAGTCTAGTGCGTCC+AGG + chr8_1:47652839-47652858 Msa1197350:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 47650787 47653901 47650787 ID=Msa1197350;Name=Msa1197350
chr8_1 mRNA 47650787 47653901 47650787 ID=Msa1197350-mRNA-1;Parent=Msa1197350;Name=Msa1197350-mRNA-1;_AED=0.04;_eAED=0.26;_QI=0|0|0.33|0.66|1|1|3|1027|183
chr8_1 exon 47650787 47651136 47650787 ID=Msa1197350-mRNA-1:exon:15117;Parent=Msa1197350-mRNA-1
chr8_1 exon 47652544 47652595 47652544 ID=Msa1197350-mRNA-1:exon:15118;Parent=Msa1197350-mRNA-1
chr8_1 exon 47652725 47653901 47652725 ID=Msa1197350-mRNA-1:exon:15119;Parent=Msa1197350-mRNA-1
chr8_1 CDS 47650787 47651136 47650787 ID=Msa1197350-mRNA-1:cds;Parent=Msa1197350-mRNA-1
chr8_1 CDS 47652544 47652595 47652544 ID=Msa1197350-mRNA-1:cds;Parent=Msa1197350-mRNA-1
chr8_1 CDS 47652725 47652874 47652725 ID=Msa1197350-mRNA-1:cds;Parent=Msa1197350-mRNA-1
chr8_1 three_prime_UTR 47652875 47653901 47652875 ID=Msa1197350-mRNA-1:three_prime_utr;Parent=Msa1197350-mRNA-1
Gene Sequence

>Msa1197350

ATGTCTAAAACAAATGTAAGAAAACAAGCTTTGGAGAGGAGCACAAGTTTCAAAGATACACAAAAAATATTGAACCAAAATTTCTTCCATAATAAAATCTTCAAGAAAGTGTACCCTATTGGACTTCAAAAAAGCACTTCTTCACTATCTTTATCTTCAGTTTCATTATCTTTGTCACAAAACTCAAATGACTCTTCTCAAGCTGATTCTTTGACTCCATTGGATGAAAGGATTTCATCAGCACTTCGTTTGATTTCAGCTTCTTCACATGAAAGGAGAGAAAGTGCAGTAGCTAAAACTATACATCAACAAAGTCCACTAGTGAGTACTGAGCCAGGGGAATTGAAAAGTGTTTTTGCTTTTTCTCTTGGTATCACTTATTCTTTTCAATCAGAGCTAGAGGTGAAAGGAGAACGAGGTGATGACCAGAGACGCCACCCAGAGGAGGAAAAGAACGAGGACAGTCGCCGGAAAGATCGAAAGGAGCGCCATACCCTCAATACGGTTTTGCTGGATGGGGCGAGTCTAGTGCGTCCAGGAAGAAGTACGTAG
Protein sequence

>Msa1197350

MSKTNVRKQALERSTSFKDTQKILNQNFFHNKIFKKVYPIGLQKSTSSLSLSSVSLSLSQNSNDSSQADSLTPLDERISSALRLISASSHERRESAVAKTIHQQSPLVSTEPGELKSVFAFSLGITYSFQSELEVKGERGDDQRRHPEEEKNEDSRRKDRKERHTLNTVLLDGASLVRPGRST*