AlfalfaGEDB | Msa1199090

M. sativa cultivar zhongmu-4 / Msa1199090

KEGG: -
GO: -
PFAM: PF14368.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1199090	A0A396GKP3	95.726	117	5	0	1	117	7	123	2.39e-72	221

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1199090	Msa1199100	0.918123	2.295154e-86	-8.615850e-47
Msa1199090	Msa1252610	0.812594	3.919765e-51	-8.615850e-47
Msa1199090	Msa1293390	0.836497	9.040573e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1199090	MtrunA17_Chr8g0368831	95.726	117	5	0	1	117	7	123	4.61e-76	221
Msa1199090	MtrunA17_Chr8g0368861	97.391	115	3	0	3	117	1	115	4.88e-76	220
Msa1199090	MtrunA17_Chr7g0240471	50.000	94	45	2	25	117	20	112	1.39e-23	87.8
Msa1199090	MtrunA17_Chr7g0240451	50.000	90	43	2	29	117	24	112	3.91e-22	84.0
Msa1199090	MtrunA17_Chr7g0240441	56.364	55	23	1	65	119	38	91	1.73e-14	63.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1199090	AT2G37870.1	71.296	108	28	1	10	117	6	110	1.76e-47	148
Msa1199090	AT5G05960.1	44.068	118	58	3	3	118	1	112	9.83e-26	93.6
Msa1199090	AT3G53980.2	44.444	90	49	1	29	118	22	110	2.94e-24	89.7
Msa1199090	AT3G53980.1	44.444	90	49	1	29	118	22	110	2.94e-24	89.7

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Find 28 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAATGGTGATGGAATTCA+AGG	0.431133	8_1:+50954004	None:intergenic
AACATTAGGAACCGACCTGC+TGG	0.527493	8_1:+50954323	Msa1199090:CDS
AAGAACTTTCCTAGCCACAG+TGG	0.766481	8_1:+50954028	Msa1199090:CDS
AATGTGGAAGGACACCAATT+GGG	0.523864	8_1:+50954105	Msa1199090:CDS
ACTCTAGCACAACAAACTGG+TGG	0.633479	8_1:-50954188	None:intergenic
AGAACAGAACAAAGACATCT+TGG	0.469439	8_1:-50954233	None:intergenic
AGGCAGCTGGAGAATGTGGA+AGG	0.611703	8_1:+50954093	Msa1199090:CDS
AGTGAGGCAGCTGGAGAATG+TGG	0.550647	8_1:+50954089	Msa1199090:CDS
ATCTTAGCTTGTTTAGCAAT+AGG	0.376748	8_1:-50954263	None:intergenic
CAACAGAAACATCACCACTG+TGG	0.756055	8_1:-50954042	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAACAGAACAAAGACATCT+TGG	-	chr8_1:50954236-50954255	None:intergenic	35.0%
TTCCTAAGAGATGCAACATT+AGG	+	chr8_1:50954309-50954328	Msa1199090:CDS	35.0%
TTCCTAATGTTGCATCTCTT+AGG	-	chr8_1:50954314-50954333	None:intergenic	35.0%
AATGTGGAAGGACACCAATT+GGG	+	chr8_1:50954105-50954124	Msa1199090:CDS	40.0%
AAGAACTTTCCTAGCCACAG+TGG	+	chr8_1:50954028-50954047	Msa1199090:CDS	45.0%
CAACAGAAACATCACCACTG+TGG	-	chr8_1:50954045-50954064	None:intergenic	45.0%
GAATGTGGAAGGACACCAAT+TGG	+	chr8_1:50954104-50954123	Msa1199090:CDS	45.0%
TTACGTACAGCACCCAAACA+TGG	-	chr8_1:50954152-50954171	None:intergenic	45.0%
ACTCTAGCACAACAAACTGG+TGG	-	chr8_1:50954191-50954210	None:intergenic	45.0%
CCAACTCTAGCACAACAAAC+TGG	-	chr8_1:50954194-50954213	None:intergenic	45.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	50954008	50954367	50954008	ID=Msa1199090;Name=Msa1199090
chr8_1	mRNA	50954008	50954367	50954008	ID=Msa1199090-mRNA-1;Parent=Msa1199090;Name=Msa1199090-mRNA-1;_AED=0.43;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|119
chr8_1	exon	50954008	50954367	50954008	ID=Msa1199090-mRNA-1:exon:16090;Parent=Msa1199090-mRNA-1
chr8_1	CDS	50954008	50954367	50954008	ID=Msa1199090-mRNA-1:cds;Parent=Msa1199090-mRNA-1

Gene Sequence	>Msa1199090 ATGGTGATGGAATTCAAGGTAAGAACTTTCCTAGCCACAGTGGTGATGTTTCTGTTGGCATCAAGTGTGTTAATTTTGGAAAGTGAGGCAGCTGGAGAATGTGGAAGGACACCAATTGGGTCAGCAGCTGCTAGTCTAAGTCCATGTTTGGGTGCTGTACGTAATGTTAGGGCAAAAGTTCCACCAGTTTGTTGTGCTAGAGTTGGTGCTTTGATTAGAACTTCTCCAAGATGTCTTTGTTCTGTTCTTTTGTCACCTATTGCTAAACAAGCTAAGATTAATCCTGCTATTGCTATCACTGTTCCTAAGAGATGCAACATTAGGAACCGACCTGCTGGAAAGAAATGTGGAAGTAAGTAA
Protein sequence	>Msa1199090 MVMEFKVRTFLATVVMFLLASSVLILESEAAGECGRTPIGSAAASLSPCLGAVRNVRAKVPPVCCARVGALIRTSPRCLCSVLLSPIAKQAKINPAIAITVPKRCNIRNRPAGKKCGSK*