Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1200460 | RHN41801.1 | 98.148 | 108 | 2 | 0 | 1 | 108 | 1 | 108 | 6.58e-70 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1200460 | sp|K7K424|DAT2D_SOYBN | 59.615 | 104 | 42 | 0 | 2 | 105 | 14 | 117 | 6.38e-39 | 135 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1037890 | Msa1200460 | 0.802328 | 5.910000e-49 | -8.615850e-47 |
| Msa1200460 | Msa1293780 | 0.820475 | 6.725132e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1200460 | MtrunA17_Chr8g0369931 | 98.148 | 108 | 2 | 0 | 1 | 108 | 1 | 108 | 6.05e-74 | 214 |
| Msa1200460 | MtrunA17_Chr1g0163481 | 67.925 | 106 | 30 | 2 | 1 | 105 | 1 | 103 | 5.01e-41 | 135 |
| Msa1200460 | MtrunA17_Chr8g0369951 | 59.434 | 106 | 42 | 1 | 1 | 105 | 1 | 106 | 1.40e-39 | 134 |
| Msa1200460 | MtrunA17_Chr5g0407691 | 58.654 | 104 | 43 | 0 | 2 | 105 | 6 | 109 | 5.87e-39 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1200460 | AT3G51520.1 | 48.387 | 93 | 48 | 0 | 13 | 105 | 11 | 103 | 1.77e-27 | 102 |
Find 17 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGGATAAGAACAGTTTAT+TGG | 0.250541 | 8_1:+53083969 | Msa1200460:CDS |
| TTCCATCTTAATCAACCTTA+TGG | 0.288730 | 8_1:+53084197 | Msa1200460:CDS |
| GTAGCATTAACTTTGTGTTT+TGG | 0.361884 | 8_1:+53083754 | Msa1200460:CDS |
| TTGCGAGGAAATGAGGTATT+TGG | 0.362850 | 8_1:+53083703 | Msa1200460:CDS |
| GTGGATAAGAACAGTTTATT+GGG | 0.373417 | 8_1:+53083970 | Msa1200460:CDS |
| CCCCATCACACTTCACTTGG+AGG | 0.407649 | 8_1:+53084163 | Msa1200460:CDS |
| AACCATAAGGTTGATTAAGA+TGG | 0.439395 | 8_1:-53084199 | None:intergenic |
| ATAGGTTAATACAAACCATA+AGG | 0.442550 | 8_1:-53084212 | None:intergenic |
| TATTTGGTGAATCATGCAAT+TGG | 0.471420 | 8_1:+53083719 | Msa1200460:CDS |
| GTTGTTTATGATTCTTCCTG+TGG | 0.497559 | 8_1:+53083951 | Msa1200460:CDS |
| TCCTCCAAGTGAAGTGTGAT+GGG | 0.501289 | 8_1:-53084164 | None:intergenic |
| TTTCCCCATCACACTTCACT+TGG | 0.516494 | 8_1:+53084160 | Msa1200460:CDS |
| AATAAACTGTTCTTATCCAC+AGG | 0.525451 | 8_1:-53083967 | None:intergenic |
| AAAAGTGTTGCGAGGAAATG+AGG | 0.537517 | 8_1:+53083696 | Msa1200460:CDS |
| ATCCTCCAAGTGAAGTGTGA+TGG | 0.611933 | 8_1:-53084165 | None:intergenic |
| GAAGAATCATAAACAACATG+AGG | 0.619475 | 8_1:-53083945 | None:intergenic |
| CCTCCAAGTGAAGTGTGATG+GGG | 0.703428 | 8_1:-53084163 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAATTAATTAAGCTAA+AGG | - | chr8_1:53084029-53084048 | None:intergenic | 10.0% |
| !! | CAATTAAAAAAGGAAATAAT+TGG | - | chr8_1:53084058-53084077 | None:intergenic | 15.0% |
| !!! | TCTTTTTATTTTTCTTCTCT+TGG | + | chr8_1:53083870-53083889 | Msa1200460:intron | 20.0% |
| !!! | TTAATTACCTGGATAATTTT+TGG | - | chr8_1:53083996-53084015 | None:intergenic | 20.0% |
| !!! | ATTATCCAGGTAATTAATTT+TGG | + | chr8_1:53083999-53084018 | Msa1200460:intron | 20.0% |
| !!! | TTATCCAGGTAATTAATTTT+GGG | + | chr8_1:53084000-53084019 | Msa1200460:intron | 20.0% |
| !! | GAATCTGAAATGAAAACAAA+AGG | - | chr8_1:53084117-53084136 | None:intergenic | 25.0% |
| ! | TATTTGGTGAATCATGCAAT+TGG | + | chr8_1:53083719-53083738 | Msa1200460:CDS | 30.0% |
| ! | GTAGCATTAACTTTGTGTTT+TGG | + | chr8_1:53083754-53083773 | Msa1200460:CDS | 30.0% |
| GAAGAATCATAAACAACATG+AGG | - | chr8_1:53083948-53083967 | None:intergenic | 30.0% | |
| AATAAACTGTTCTTATCCAC+AGG | - | chr8_1:53083970-53083989 | None:intergenic | 30.0% | |
| TGTGGATAAGAACAGTTTAT+TGG | + | chr8_1:53083969-53083988 | Msa1200460:CDS | 30.0% | |
| GTGGATAAGAACAGTTTATT+GGG | + | chr8_1:53083970-53083989 | Msa1200460:CDS | 30.0% | |
| GAAACCCAAAATTAATTACC+TGG | - | chr8_1:53084007-53084026 | None:intergenic | 30.0% | |
| TTCCATCTTAATCAACCTTA+TGG | + | chr8_1:53084197-53084216 | Msa1200460:CDS | 30.0% | |
| ! | AACCATAAGGTTGATTAAGA+TGG | - | chr8_1:53084202-53084221 | None:intergenic | 30.0% |
| !! | AAAGTGCTTTTGAGAGAGAA+AGG | - | chr8_1:53083825-53083844 | None:intergenic | 35.0% |
| !!! | TGGCTCAAACACTTTTTTGA+TGG | + | chr8_1:53083890-53083909 | Msa1200460:intron | 35.0% |
| ! | AATTTGCATGTCAGGCTTTT+TGG | + | chr8_1:53083919-53083938 | Msa1200460:intron | 35.0% |
| GTTGTTTATGATTCTTCCTG+TGG | + | chr8_1:53083951-53083970 | Msa1200460:CDS | 35.0% | |
| TATTGGGCCAAAAATTATCC+AGG | + | chr8_1:53083986-53084005 | Msa1200460:CDS | 35.0% | |
| !! | AAAAGTGTTGCGAGGAAATG+AGG | + | chr8_1:53083696-53083715 | Msa1200460:CDS | 40.0% |
| ! | TTGCGAGGAAATGAGGTATT+TGG | + | chr8_1:53083703-53083722 | Msa1200460:CDS | 40.0% |
| GGTGTTTCAATTTGCATGTC+AGG | + | chr8_1:53083911-53083930 | Msa1200460:intron | 40.0% | |
| TTTCCCCATCACACTTCACT+TGG | + | chr8_1:53084160-53084179 | Msa1200460:CDS | 45.0% | |
| TCCTCCAAGTGAAGTGTGAT+GGG | - | chr8_1:53084167-53084186 | None:intergenic | 45.0% | |
| ATCCTCCAAGTGAAGTGTGA+TGG | - | chr8_1:53084168-53084187 | None:intergenic | 45.0% | |
| !!! | TTTTAATTTACAATTAAAAA+AGG | - | chr8_1:53084068-53084087 | None:intergenic | 5.0% |
| CCTCCAAGTGAAGTGTGATG+GGG | - | chr8_1:53084166-53084185 | None:intergenic | 50.0% | |
| CCCCATCACACTTCACTTGG+AGG | + | chr8_1:53084163-53084182 | Msa1200460:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 53083691 | 53084229 | 53083691 | ID=Msa1200460;Name=Msa1200460 |
| chr8_1 | mRNA | 53083691 | 53084229 | 53083691 | ID=Msa1200460-mRNA-1;Parent=Msa1200460;Name=Msa1200460-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.33|1|1|1|3|0|108 |
| chr8_1 | exon | 53083691 | 53083845 | 53083691 | ID=Msa1200460-mRNA-1:exon:16957;Parent=Msa1200460-mRNA-1 |
| chr8_1 | exon | 53083933 | 53084007 | 53083933 | ID=Msa1200460-mRNA-1:exon:16958;Parent=Msa1200460-mRNA-1 |
| chr8_1 | exon | 53084133 | 53084229 | 53084133 | ID=Msa1200460-mRNA-1:exon:16959;Parent=Msa1200460-mRNA-1 |
| chr8_1 | CDS | 53083691 | 53083845 | 53083691 | ID=Msa1200460-mRNA-1:cds;Parent=Msa1200460-mRNA-1 |
| chr8_1 | CDS | 53083933 | 53084007 | 53083933 | ID=Msa1200460-mRNA-1:cds;Parent=Msa1200460-mRNA-1 |
| chr8_1 | CDS | 53084133 | 53084229 | 53084133 | ID=Msa1200460-mRNA-1:cds;Parent=Msa1200460-mRNA-1 |
| Gene Sequence |
| Protein sequence |
