AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1037890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACACAAGATTTCTTCGTTT+TGG 0.224044 7_1:+74636065 Msa1037890:CDS
CAATCCAGTGGATGCAAAAT+TGG 0.229600 7_1:-74636551 None:intergenic
GTTGGAGAGAAGCTTGTAAT+TGG 0.310945 7_1:+74636526 Msa1037890:three_prime_UTR
CATCCACTGGATTGTATTCA+AGG 0.353753 7_1:+74636560 Msa1037890:three_prime_UTR
GTTGTAATAATAAGCTTGGT+TGG 0.449013 7_1:+74636028 None:intergenic
CGGATGAGTAGCGTCAACTA+TGG 0.473941 7_1:-74636138 None:intergenic
TATTTGTGCCCACTACTAGT+TGG 0.475875 7_1:+74636508 Msa1037890:three_prime_UTR
AGCTTCTCTCCAACTAGTAG+TGG 0.538262 7_1:-74636517 None:intergenic
TCGTCATCGTCTTCAGTATG+TGG 0.544092 7_1:+74637215 Msa1037890:three_prime_UTR
AAAACGAAGAAATCTTGTGT+TGG 0.585244 7_1:-74636063 None:intergenic
AGTAGCGTCAACTATGGCAG+TGG 0.617409 7_1:-74636132 None:intergenic
GCTTCTCTCCAACTAGTAGT+GGG 0.634311 7_1:-74636516 None:intergenic
AGATTCTTTATAGTGGACAG+TGG 0.675519 7_1:+74636089 Msa1037890:CDS
AAACCTTGAATACAATCCAG+TGG 0.710261 7_1:-74636563 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTGTTATTTACAGGTT+TGG + chr7_1:74636471-74636490 Msa1037890:intron 20.0%
!! TTCTCAATCAAAGTTATATA+TGG - chr7_1:74637113-74637132 None:intergenic 20.0%
!!! ATTTTTGTTATTTACAGGTT+TGG + chr7_1:74636471-74636490 Msa1037890:intron 20.0%
!! TTCTCAATCAAAGTTATATA+TGG - chr7_1:74637113-74637132 None:intergenic 20.0%
!!! GCATCATTTTTGTTATTTAC+AGG + chr7_1:74636466-74636485 Msa1037890:intron 25.0%
!!! TTACCAAACTATAGTTTTGA+TGG + chr7_1:74636642-74636661 Msa1037890:intron 25.0%
!!! TACCAAACTATAGTTTTGAT+GGG + chr7_1:74636643-74636662 Msa1037890:intron 25.0%
! TGTAATGTTTGGATACTAAA+GGG + chr7_1:74636813-74636832 Msa1037890:intron 25.0%
!!! TGTTATTTCATCCATCTTAT+GGG - chr7_1:74636875-74636894 None:intergenic 25.0%
!!! TTGTTATTTCATCCATCTTA+TGG - chr7_1:74636876-74636895 None:intergenic 25.0%
! GAAAAGTCAAGAAAAAGATT+TGG + chr7_1:74637142-74637161 Msa1037890:intron 25.0%
!!! GCATCATTTTTGTTATTTAC+AGG + chr7_1:74636466-74636485 Msa1037890:intron 25.0%
!!! TTACCAAACTATAGTTTTGA+TGG + chr7_1:74636642-74636661 Msa1037890:intron 25.0%
!!! TACCAAACTATAGTTTTGAT+GGG + chr7_1:74636643-74636662 Msa1037890:intron 25.0%
! TGTAATGTTTGGATACTAAA+GGG + chr7_1:74636813-74636832 Msa1037890:intron 25.0%
!!! TGTTATTTCATCCATCTTAT+GGG - chr7_1:74636875-74636894 None:intergenic 25.0%
!!! TTGTTATTTCATCCATCTTA+TGG - chr7_1:74636876-74636895 None:intergenic 25.0%
! GAAAAGTCAAGAAAAAGATT+TGG + chr7_1:74637142-74637161 Msa1037890:intron 25.0%
AAAACGAAGAAATCTTGTGT+TGG - chr7_1:74636066-74636085 None:intergenic 30.0%
! AACACAAGATTTCTTCGTTT+TGG + chr7_1:74636065-74636084 Msa1037890:CDS 30.0%
! ACACAAGATTTCTTCGTTTT+GGG + chr7_1:74636066-74636085 Msa1037890:CDS 30.0%
!! TTTTGGGAGATTCTTTATAG+TGG + chr7_1:74636082-74636101 Msa1037890:CDS 30.0%
CCATTAGAAAAATGTTCACT+AGG - chr7_1:74636188-74636207 None:intergenic 30.0%
! CCTAGTGAACATTTTTCTAA+TGG + chr7_1:74636185-74636204 Msa1037890:intron 30.0%
! ATGATTAGTCGATATACATG+TGG - chr7_1:74636379-74636398 None:intergenic 30.0%
TACCCATCAAAACTATAGTT+TGG - chr7_1:74636648-74636667 None:intergenic 30.0%
! CTTGCCTTTTGTGAAATTAT+CGG - chr7_1:74636732-74636751 None:intergenic 30.0%
CAATGTGATACTGTAATGTT+TGG + chr7_1:74636802-74636821 Msa1037890:intron 30.0%
CTGTAATGTTTGGATACTAA+AGG + chr7_1:74636812-74636831 Msa1037890:intron 30.0%
AAAACGAAGAAATCTTGTGT+TGG - chr7_1:74636066-74636085 None:intergenic 30.0%
! AACACAAGATTTCTTCGTTT+TGG + chr7_1:74636065-74636084 Msa1037890:CDS 30.0%
! ACACAAGATTTCTTCGTTTT+GGG + chr7_1:74636066-74636085 Msa1037890:CDS 30.0%
!! TTTTGGGAGATTCTTTATAG+TGG + chr7_1:74636082-74636101 Msa1037890:CDS 30.0%
CCATTAGAAAAATGTTCACT+AGG - chr7_1:74636188-74636207 None:intergenic 30.0%
! CCTAGTGAACATTTTTCTAA+TGG + chr7_1:74636185-74636204 Msa1037890:intron 30.0%
! ATGATTAGTCGATATACATG+TGG - chr7_1:74636379-74636398 None:intergenic 30.0%
TACCCATCAAAACTATAGTT+TGG - chr7_1:74636648-74636667 None:intergenic 30.0%
! CTTGCCTTTTGTGAAATTAT+CGG - chr7_1:74636732-74636751 None:intergenic 30.0%
CAATGTGATACTGTAATGTT+TGG + chr7_1:74636802-74636821 Msa1037890:intron 30.0%
CTGTAATGTTTGGATACTAA+AGG + chr7_1:74636812-74636831 Msa1037890:intron 30.0%
!! AGATTCTTTATAGTGGACAG+TGG + chr7_1:74636089-74636108 Msa1037890:CDS 35.0%
AAATGTTCACTAGGTATGTC+TGG - chr7_1:74636179-74636198 None:intergenic 35.0%
CGATTAAATCGAGGATGTTT+GGG - chr7_1:74636211-74636230 None:intergenic 35.0%
CCAAACATCCTCGATTTAAT+CGG + chr7_1:74636209-74636228 Msa1037890:intron 35.0%
AAACCTTGAATACAATCCAG+TGG - chr7_1:74636566-74636585 None:intergenic 35.0%
TATCGGACAGATTTATGATG+AGG - chr7_1:74636715-74636734 None:intergenic 35.0%
CTGTCCGATAATTTCACAAA+AGG + chr7_1:74636725-74636744 Msa1037890:intron 35.0%
TATGCTTGCAATTGTTTGTG+AGG - chr7_1:74636772-74636791 None:intergenic 35.0%
TCGAAGGAAACAATATCTCA+GGG - chr7_1:74637172-74637191 None:intergenic 35.0%
!! AGATTCTTTATAGTGGACAG+TGG + chr7_1:74636089-74636108 Msa1037890:CDS 35.0%
AAATGTTCACTAGGTATGTC+TGG - chr7_1:74636179-74636198 None:intergenic 35.0%
CGATTAAATCGAGGATGTTT+GGG - chr7_1:74636211-74636230 None:intergenic 35.0%
CCAAACATCCTCGATTTAAT+CGG + chr7_1:74636209-74636228 Msa1037890:intron 35.0%
AAACCTTGAATACAATCCAG+TGG - chr7_1:74636566-74636585 None:intergenic 35.0%
TATCGGACAGATTTATGATG+AGG - chr7_1:74636715-74636734 None:intergenic 35.0%
CTGTCCGATAATTTCACAAA+AGG + chr7_1:74636725-74636744 Msa1037890:intron 35.0%
TATGCTTGCAATTGTTTGTG+AGG - chr7_1:74636772-74636791 None:intergenic 35.0%
TCGAAGGAAACAATATCTCA+GGG - chr7_1:74637172-74637191 None:intergenic 35.0%
TCTGGCTAATCAAGACCATA+CGG - chr7_1:74636161-74636180 None:intergenic 40.0%
CCGATTAAATCGAGGATGTT+TGG - chr7_1:74636212-74636231 None:intergenic 40.0%
! CGATCATACCGATTAAATCG+AGG - chr7_1:74636220-74636239 None:intergenic 40.0%
ATGTCTCATGTCACATGTGT+AGG + chr7_1:74636426-74636445 Msa1037890:intron 40.0%
TGTCTCATGTCACATGTGTA+GGG + chr7_1:74636427-74636446 Msa1037890:intron 40.0%
TATTTGTGCCCACTACTAGT+TGG + chr7_1:74636508-74636527 Msa1037890:three_prime_UTR 40.0%
GTTGGAGAGAAGCTTGTAAT+TGG + chr7_1:74636526-74636545 Msa1037890:three_prime_UTR 40.0%
CAATCCAGTGGATGCAAAAT+TGG - chr7_1:74636554-74636573 None:intergenic 40.0%
CATCCACTGGATTGTATTCA+AGG + chr7_1:74636560-74636579 Msa1037890:three_prime_UTR 40.0%
CTCGAAGGAAACAATATCTC+AGG - chr7_1:74637173-74637192 None:intergenic 40.0%
GATATTGTTTCCTTCGAGCA+TGG + chr7_1:74637175-74637194 Msa1037890:intron 40.0%
TCTGGCTAATCAAGACCATA+CGG - chr7_1:74636161-74636180 None:intergenic 40.0%
CCGATTAAATCGAGGATGTT+TGG - chr7_1:74636212-74636231 None:intergenic 40.0%
! CGATCATACCGATTAAATCG+AGG - chr7_1:74636220-74636239 None:intergenic 40.0%
ATGTCTCATGTCACATGTGT+AGG + chr7_1:74636426-74636445 Msa1037890:intron 40.0%
TGTCTCATGTCACATGTGTA+GGG + chr7_1:74636427-74636446 Msa1037890:intron 40.0%
TATTTGTGCCCACTACTAGT+TGG + chr7_1:74636508-74636527 Msa1037890:three_prime_UTR 40.0%
GTTGGAGAGAAGCTTGTAAT+TGG + chr7_1:74636526-74636545 Msa1037890:three_prime_UTR 40.0%
CAATCCAGTGGATGCAAAAT+TGG - chr7_1:74636554-74636573 None:intergenic 40.0%
CATCCACTGGATTGTATTCA+AGG + chr7_1:74636560-74636579 Msa1037890:three_prime_UTR 40.0%
CTCGAAGGAAACAATATCTC+AGG - chr7_1:74637173-74637192 None:intergenic 40.0%
GATATTGTTTCCTTCGAGCA+TGG + chr7_1:74637175-74637194 Msa1037890:intron 40.0%
GCTTCTCTCCAACTAGTAGT+GGG - chr7_1:74636519-74636538 None:intergenic 45.0%
AGCTTCTCTCCAACTAGTAG+TGG - chr7_1:74636520-74636539 None:intergenic 45.0%
GCTTGCAATTGTTTGTGAGG+AGG - chr7_1:74636769-74636788 None:intergenic 45.0%
CATCCATCTTATGGGATGCA+AGG - chr7_1:74636867-74636886 None:intergenic 45.0%
TCGTCATCGTCTTCAGTATG+TGG + chr7_1:74637215-74637234 Msa1037890:three_prime_UTR 45.0%
GCTTCTCTCCAACTAGTAGT+GGG - chr7_1:74636519-74636538 None:intergenic 45.0%
AGCTTCTCTCCAACTAGTAG+TGG - chr7_1:74636520-74636539 None:intergenic 45.0%
GCTTGCAATTGTTTGTGAGG+AGG - chr7_1:74636769-74636788 None:intergenic 45.0%
CATCCATCTTATGGGATGCA+AGG - chr7_1:74636867-74636886 None:intergenic 45.0%
TCGTCATCGTCTTCAGTATG+TGG + chr7_1:74637215-74637234 Msa1037890:three_prime_UTR 45.0%
AGTAGCGTCAACTATGGCAG+TGG - chr7_1:74636135-74636154 None:intergenic 50.0%
CGGATGAGTAGCGTCAACTA+TGG - chr7_1:74636141-74636160 None:intergenic 50.0%
GTTGACGCTACTCATCCGTA+TGG + chr7_1:74636143-74636162 Msa1037890:three_prime_UTR 50.0%
! GGTGCCAATTTTGCATCCAC+TGG + chr7_1:74636547-74636566 Msa1037890:three_prime_UTR 50.0%
CTGCCTTGCATCCCATAAGA+TGG + chr7_1:74636861-74636880 Msa1037890:intron 50.0%
CATCTGCAAACCATGCTCGA+AGG - chr7_1:74637188-74637207 None:intergenic 50.0%
! CTTCGAGCATGGTTTGCAGA+TGG + chr7_1:74637186-74637205 Msa1037890:intron 50.0%
AGTAGCGTCAACTATGGCAG+TGG - chr7_1:74636135-74636154 None:intergenic 50.0%
CGGATGAGTAGCGTCAACTA+TGG - chr7_1:74636141-74636160 None:intergenic 50.0%
GTTGACGCTACTCATCCGTA+TGG + chr7_1:74636143-74636162 Msa1037890:three_prime_UTR 50.0%
! GGTGCCAATTTTGCATCCAC+TGG + chr7_1:74636547-74636566 Msa1037890:three_prime_UTR 50.0%
CTGCCTTGCATCCCATAAGA+TGG + chr7_1:74636861-74636880 Msa1037890:intron 50.0%
CATCTGCAAACCATGCTCGA+AGG - chr7_1:74637188-74637207 None:intergenic 50.0%
! CTTCGAGCATGGTTTGCAGA+TGG + chr7_1:74637186-74637205 Msa1037890:intron 50.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 74636042 74637245 74636042 ID=Msa1037890;Name=Msa1037890
chr7_1 mRNA 74636042 74637245 74636042 ID=Msa1037890-mRNA-1;Parent=Msa1037890;Name=Msa1037890-mRNA-1;_AED=0.65;_eAED=0.90;_QI=13|0|0|0.66|0|0|3|185|19
chr7_1 exon 74636042 74636164 74636042 ID=Msa1037890-mRNA-1:exon:18858;Parent=Msa1037890-mRNA-1
chr7_1 exon 74636488 74636581 74636488 ID=Msa1037890-mRNA-1:exon:18859;Parent=Msa1037890-mRNA-1
chr7_1 exon 74637205 74637245 74637205 ID=Msa1037890-mRNA-1:exon:18860;Parent=Msa1037890-mRNA-1
chr7_1 five_prime_UTR 74636042 74636054 74636042 ID=Msa1037890-mRNA-1:five_prime_utr;Parent=Msa1037890-mRNA-1
chr7_1 CDS 74636055 74636114 74636055 ID=Msa1037890-mRNA-1:cds;Parent=Msa1037890-mRNA-1
chr7_1 three_prime_UTR 74636115 74636164 74636115 ID=Msa1037890-mRNA-1:three_prime_utr;Parent=Msa1037890-mRNA-1
chr7_1 three_prime_UTR 74636488 74636581 74636488 ID=Msa1037890-mRNA-1:three_prime_utr;Parent=Msa1037890-mRNA-1
chr7_1 three_prime_UTR 74637205 74637245 74637205 ID=Msa1037890-mRNA-1:three_prime_utr;Parent=Msa1037890-mRNA-1
Gene Sequence

>Msa1037890

ATGCTCAACCAACACAAGATTTCTTCGTTTTGGGAGATTCTTTATAGTGGACAGTGGTAA

Protein sequence

>Msa1037890

MLNQHKISSFWEILYSGQW*