AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1204960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204960 MtrunA17_Chr4g0053911 97.854 233 5 0 1 233 1 233 1.59e-169 466
Msa1204960 MtrunA17_Chr5g0401401 70.588 238 58 5 1 233 1 231 1.40e-110 317
Msa1204960 MtrunA17_Chr7g0215281 61.417 127 46 1 1 124 3 129 5.77e-55 178
Msa1204960 MtrunA17_Chr6g0457431 55.319 141 51 1 1 141 3 131 7.98e-53 167
Msa1204960 MtrunA17_Chr1g0207471 63.566 129 43 1 1 129 1 125 2.79e-52 167
Msa1204960 MtrunA17_Chr6g0469271 60.550 109 41 1 1 107 3 111 1.56e-46 154
Msa1204960 MtrunA17_Chr6g0470651 51.667 120 54 3 1 118 3 120 9.31e-42 142
Msa1204960 MtrunA17_Chr6g0470631 50.833 120 55 3 1 118 3 120 1.29e-40 139
Msa1204960 MtrunA17_Chr1g0209841 50.000 120 46 3 1 118 3 110 1.89e-37 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204960 AT4G37540.1 58.233 249 79 8 1 233 1 240 2.25e-83 248
Msa1204960 AT3G49940.1 57.540 252 81 7 1 232 1 246 5.94e-82 245
Msa1204960 AT5G67420.2 54.545 253 92 7 1 233 1 250 4.77e-75 228
Msa1204960 AT5G67420.1 54.545 253 92 7 1 233 1 250 4.77e-75 228
Msa1204960 AT1G67100.1 66.667 111 37 0 1 111 3 113 7.70e-54 173
Msa1204960 AT3G02550.1 49.718 177 73 4 1 173 3 167 1.22e-51 168
Msa1204960 AT1G68510.1 63.462 104 38 0 1 104 3 106 2.57e-48 159

Find 105 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACTCATATTTCTATTT+TGG 0.129107 8_1:+60921288 None:intergenic
AAGATTCGCGATCCGAATTT+CGG 0.249148 8_1:-60920741 Msa1204960:CDS
GTAGCGTGGCCTTGAGCTTC+TGG 0.259138 8_1:+60921191 None:intergenic
TTACCTGGTCTCTGTGGTTC+GGG 0.275915 8_1:+60921110 None:intergenic
GTTGGGGTATAAATAAATAA+AGG 0.296968 8_1:+60921433 None:intergenic
ACCAGCTCGACCAAAGAATT+TGG 0.305659 8_1:+60921157 None:intergenic
GACTTCTTTGGACTGGAAAC+TGG 0.306937 8_1:-60920905 Msa1204960:CDS
ATTTGATATTCCACGGTTAC+AGG 0.313336 8_1:+60920234 None:intergenic
ACGGTGCTGTTGGACTTCTT+TGG 0.315165 8_1:-60920917 Msa1204960:CDS
TGAAAGTGGTTTAGGAATAA+TGG 0.315956 8_1:+60921380 None:intergenic
GAATAGAATGAAAGTGGTTT+AGG 0.325204 8_1:+60921372 None:intergenic
TTTATCGGAGCTGACACTTC+TGG 0.333923 8_1:-60920829 Msa1204960:CDS
AGATTCGCGATCCGAATTTC+GGG 0.347093 8_1:-60920740 Msa1204960:CDS
ACGGTGGCGCTGATGCTATT+TGG 0.350213 8_1:-60920764 Msa1204960:CDS
ACGACGGCGTGTTTAGAAAC+TGG 0.362464 8_1:-60920534 Msa1204960:CDS
TCTAGTAATAAGGTGTGTTC+TGG 0.372758 8_1:-60920702 Msa1204960:CDS
TGGCACGCTTAGGCCTTTAT+CGG 0.389750 8_1:-60920844 Msa1204960:CDS
ATTACCTGGTCTCTGTGGTT+CGG 0.410585 8_1:+60921109 None:intergenic
GAGCTCGCCAGCCCGAAATT+CGG 0.420698 8_1:+60920729 None:intergenic
TTGGGGTATAAATAAATAAA+GGG 0.424281 8_1:+60921434 None:intergenic
GAGCTCACGATCTAGTAATA+AGG 0.427686 8_1:-60920712 Msa1204960:CDS
AAGAGAGAATAGAATGAAAG+TGG 0.431602 8_1:+60921366 None:intergenic
AGTAAACTATGGTTGAGTTA+AGG 0.435665 8_1:+60921489 None:intergenic
GTCGATGACGTCGGAGGAAT+CGG 0.439790 8_1:-60920562 Msa1204960:CDS
AGAAGTGTCAGCTCCGATAA+AGG 0.449767 8_1:+60920831 None:intergenic
CAGAAAATCCTCATATGAAA+AGG 0.454062 8_1:+60920466 None:intergenic
AGAAGAAGAGGAGGTGTGGT+TGG 0.462946 8_1:+60921465 None:intergenic
GCCAAATTCTTTGGTCGAGC+TGG 0.464513 8_1:-60921158 Msa1204960:CDS
GCTGTTGGACTTCTTTGGAC+TGG 0.482667 8_1:-60920912 Msa1204960:CDS
GACGTCATCGACGGTGAACC+AGG 0.485410 8_1:+60920573 None:intergenic
ATGCGAATTGAAGTGATGTT+GGG 0.486771 8_1:+60921416 None:intergenic
GTGTGGTTGGTAGTAAACTA+TGG 0.487162 8_1:+60921478 None:intergenic
TTCTCTAGAGAGAAAGATGA+TGG 0.491224 8_1:-60920316 Msa1204960:three_prime_UTR
AGGGGAGGAAGGAGAAGAAG+AGG 0.493231 8_1:+60921453 None:intergenic
AATAGAAATATGAGTTGCAA+TGG 0.497518 8_1:-60921284 Msa1204960:exon
TTGTGGTGAAAAGAGTAAAA+CGG 0.498145 8_1:-60920381 Msa1204960:three_prime_UTR
GCAGTGGAGGAACGCCGGCC+TGG 0.500212 8_1:-60920591 Msa1204960:CDS
AATGCGAATTGAAGTGATGT+TGG 0.500278 8_1:+60921415 None:intergenic
TCTCTAGAGAAATCCTGAAC+CGG 0.501718 8_1:+60920328 None:intergenic
CGACGGCGTGTTTAGAAACT+GGG 0.509803 8_1:-60920533 Msa1204960:CDS
TTCGGGGACGTTGGAAATGA+AGG 0.510532 8_1:+60921127 None:intergenic
GTTTCGATCCATTGCAAACA+AGG 0.513363 8_1:+60921215 None:intergenic
AGTCCAACAGCACCGTTCAC+AGG 0.514245 8_1:+60920924 None:intergenic
AGTTAAGGTTAGTTGCAAGT+GGG 0.519707 8_1:+60921504 None:intergenic
TAAATAAATAAAGGGGAGGA+AGG 0.524788 8_1:+60921442 None:intergenic
ATTTAATATTTGATATTCCA+CGG 0.529132 8_1:+60920227 None:intergenic
AAGGAGAAGAAGAGGAGGTG+TGG 0.529924 8_1:+60921461 None:intergenic
ATAGAAATATGAGTTGCAAT+GGG 0.530799 8_1:-60921283 Msa1204960:exon
AAATCAAGATTAATCGCCGC+TGG 0.534004 8_1:+60920642 None:intergenic
GTTAAGGTTAGTTGCAAGTG+GGG 0.540885 8_1:+60921505 None:intergenic
TGGAAGCACACGGAGGAGAT+CGG 0.542304 8_1:-60920682 Msa1204960:CDS
GGTGGAGTCACGGTGGTGAT+CGG 0.543236 8_1:-60920500 Msa1204960:CDS
GGAGGAAGGAGAAGAAGAGG+AGG 0.543266 8_1:+60921456 None:intergenic
TAGAAACTGGGATTGGAGAT+CGG 0.545733 8_1:-60920521 Msa1204960:CDS
ATTGGAGATCGGTGGAGTCA+CGG 0.549508 8_1:-60920510 Msa1204960:CDS
GAGTTAAGGTTAGTTGCAAG+TGG 0.557508 8_1:+60921503 None:intergenic
AACCCTGTGAACGGTGCTGT+TGG 0.557626 8_1:-60920927 Msa1204960:CDS
GCGTGTTTAGAAACTGGGAT+TGG 0.557756 8_1:-60920528 Msa1204960:CDS
GTGGTTTAGGAATAATGGAA+TGG 0.559576 8_1:+60921385 None:intergenic
AGCAAAACAGAGAAACGTTA+CGG 0.559764 8_1:+60920415 None:intergenic
TTCTTCGCGGTGGCACGCTT+AGG 0.561098 8_1:-60920854 Msa1204960:CDS
GGGTGCCGAGTTCTTCGAAA+GGG 0.563138 8_1:-60921263 Msa1204960:CDS
GTTTGAAGCGTGTGGAAGAA+CGG 0.563405 8_1:-60920952 Msa1204960:CDS
TCTCTGTGGTTCGGGGACGT+TGG 0.563762 8_1:+60921118 None:intergenic
TCGCGATCCGAATTTCGGGC+TGG 0.563771 8_1:-60920736 Msa1204960:CDS
TTAGGAATAATGGAATGGAA+TGG 0.568834 8_1:+60921390 None:intergenic
TTAAGGTTAGTTGCAAGTGG+GGG 0.577324 8_1:+60921506 None:intergenic
TGCCACTGATGAAGCTTCTG+AGG 0.578795 8_1:-60920793 Msa1204960:CDS
TCATCGACGGTGAACCAGGC+CGG 0.580860 8_1:+60920577 None:intergenic
AGAAGGCAGTGGAGGAACGC+CGG 0.585660 8_1:-60920596 Msa1204960:CDS
GAAGCTTCTGAGGCGGAAGA+CGG 0.587046 8_1:-60920783 Msa1204960:CDS
CACTGATGAAGCTTCTGAGG+CGG 0.596477 8_1:-60920790 Msa1204960:CDS
TCTCTACTGTTTGAAGCGTG+TGG 0.600037 8_1:-60920960 Msa1204960:CDS
GTCCAACAGCACCGTTCACA+GGG 0.600320 8_1:+60920925 None:intergenic
AGAGAAAGATGATGGTTCGT+CGG 0.604350 8_1:-60920308 Msa1204960:three_prime_UTR
GCTTCATCAGTGGCAGTGAG+AGG 0.608069 8_1:+60920801 None:intergenic
TTTGCTTACAATACTCATTG+TGG 0.609288 8_1:-60920398 Msa1204960:three_prime_UTR
CGCCTCAGAAGCTTCATCAG+TGG 0.613409 8_1:+60920791 None:intergenic
GTCCCCGAACCACAGAGACC+AGG 0.617602 8_1:-60921113 Msa1204960:intron
GAGATCGGAGGAGTTTGTGA+AGG 0.623682 8_1:-60920667 Msa1204960:CDS
GCGGTTGAGACAGTTCTTCG+CGG 0.626359 8_1:-60920867 Msa1204960:CDS
AAATAATTACCTGGTCTCTG+TGG 0.626571 8_1:+60921104 None:intergenic
TACCTGGTCTCTGTGGTTCG+GGG 0.637914 8_1:+60921111 None:intergenic
GCTTCTGAGGCGGAAGACGG+TGG 0.641101 8_1:-60920780 Msa1204960:CDS
TGCGAATTGAAGTGATGTTG+GGG 0.644823 8_1:+60921417 None:intergenic
GATTCCTCCGACGTCATCGA+CGG 0.648497 8_1:+60920564 None:intergenic
TGGGTGCCGAGTTCTTCGAA+AGG 0.651709 8_1:-60921264 Msa1204960:CDS
TGGGGTATAAATAAATAAAG+GGG 0.654291 8_1:+60921435 None:intergenic
TGGTTCACCGTCGATGACGT+CGG 0.655686 8_1:-60920571 Msa1204960:CDS
GATGTGTAAACCTGTAACCG+TGG 0.659840 8_1:-60920244 Msa1204960:three_prime_UTR
AGGTGTGTTCTGGAAGCACA+CGG 0.660146 8_1:-60920692 Msa1204960:CDS
GGAGGAATCGGTGACGACGA+CGG 0.660492 8_1:-60920550 Msa1204960:CDS
TGCAACCCTTTCGAAGAACT+CGG 0.661053 8_1:+60921258 None:intergenic
CTGGCACGTGTGTCAAGCAG+CGG 0.661568 8_1:-60920886 Msa1204960:CDS
AGAACGGTTAACCCTGTGAA+CGG 0.663812 8_1:-60920936 Msa1204960:CDS
AAGCACACGGAGGAGATCGG+AGG 0.664572 8_1:-60920679 Msa1204960:CDS
GGAGTTTGTGAAGGTTCCAG+CGG 0.666158 8_1:-60920658 Msa1204960:CDS
GTTGAGACAGTTCTTCGCGG+TGG 0.669818 8_1:-60920864 Msa1204960:CDS
GGTATAAATAAATAAAGGGG+AGG 0.672012 8_1:+60921438 None:intergenic
ATCGAAACTCCAGAAGCTCA+AGG 0.686071 8_1:-60921200 Msa1204960:CDS
AAACTGGGATTGGAGATCGG+TGG 0.696279 8_1:-60920518 Msa1204960:CDS
TTCACCGTCGATGACGTCGG+AGG 0.699966 8_1:-60920568 Msa1204960:CDS
TGGCTACGAAAACAGTAGCG+TGG 0.700795 8_1:+60921177 None:intergenic
GGAGATCGGTGGAGTCACGG+TGG 0.745894 8_1:-60920507 Msa1204960:CDS
TGTGTTCTGGAAGCACACGG+AGG 0.795756 8_1:-60920689 Msa1204960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATATTTGATATTCCA+CGG + chr8_1:60921501-60921520 None:intergenic 15.0%
!! ATTTAATATTTGATATTCCA+CGG + chr8_1:60921501-60921520 None:intergenic 15.0%
!! TTGGGGTATAAATAAATAAA+GGG + chr8_1:60920294-60920313 None:intergenic 20.0%
!! CAATCTTTCTCTTATATTTA+CGG + chr8_1:60920662-60920681 None:intergenic 20.0%
!! TTGGGGTATAAATAAATAAA+GGG + chr8_1:60920294-60920313 None:intergenic 20.0%
!! CAATCTTTCTCTTATATTTA+CGG + chr8_1:60920662-60920681 None:intergenic 20.0%
! TGGGGTATAAATAAATAAAG+GGG + chr8_1:60920293-60920312 None:intergenic 25.0%
! GTTGGGGTATAAATAAATAA+AGG + chr8_1:60920295-60920314 None:intergenic 25.0%
!!! TGCAACTCATATTTCTATTT+TGG + chr8_1:60920440-60920459 None:intergenic 25.0%
! AATAGAAATATGAGTTGCAA+TGG - chr8_1:60920441-60920460 Msa1204960:three_prime_UTR 25.0%
! ATAGAAATATGAGTTGCAAT+GGG - chr8_1:60920442-60920461 Msa1204960:three_prime_UTR 25.0%
! CGTAAATATAAGAGAAAGAT+TGG - chr8_1:60920660-60920679 Msa1204960:CDS 25.0%
! TGGGGTATAAATAAATAAAG+GGG + chr8_1:60920293-60920312 None:intergenic 25.0%
! GTTGGGGTATAAATAAATAA+AGG + chr8_1:60920295-60920314 None:intergenic 25.0%
!!! TGCAACTCATATTTCTATTT+TGG + chr8_1:60920440-60920459 None:intergenic 25.0%
! AATAGAAATATGAGTTGCAA+TGG - chr8_1:60920441-60920460 Msa1204960:three_prime_UTR 25.0%
! ATAGAAATATGAGTTGCAAT+GGG - chr8_1:60920442-60920461 Msa1204960:three_prime_UTR 25.0%
! CGTAAATATAAGAGAAAGAT+TGG - chr8_1:60920660-60920679 Msa1204960:CDS 25.0%
AGTAAACTATGGTTGAGTTA+AGG + chr8_1:60920239-60920258 None:intergenic 30.0%
TAAATAAATAAAGGGGAGGA+AGG + chr8_1:60920286-60920305 None:intergenic 30.0%
GGTATAAATAAATAAAGGGG+AGG + chr8_1:60920290-60920309 None:intergenic 30.0%
! TTAGGAATAATGGAATGGAA+TGG + chr8_1:60920338-60920357 None:intergenic 30.0%
TGAAAGTGGTTTAGGAATAA+TGG + chr8_1:60920348-60920367 None:intergenic 30.0%
GAATAGAATGAAAGTGGTTT+AGG + chr8_1:60920356-60920375 None:intergenic 30.0%
AAGAGAGAATAGAATGAAAG+TGG + chr8_1:60920362-60920381 None:intergenic 30.0%
AGACCAGGTAATTATTTAAC+CGG - chr8_1:60920627-60920646 Msa1204960:CDS 30.0%
CAGAAAATCCTCATATGAAA+AGG + chr8_1:60921262-60921281 None:intergenic 30.0%
TTTGCTTACAATACTCATTG+TGG - chr8_1:60921327-60921346 Msa1204960:five_prime_UTR 30.0%
TTGTGGTGAAAAGAGTAAAA+CGG - chr8_1:60921344-60921363 Msa1204960:five_prime_UTR 30.0%
!! AGTAAAACGGTTTTGTAGTT+CGG - chr8_1:60921357-60921376 Msa1204960:five_prime_UTR 30.0%
AGTAAACTATGGTTGAGTTA+AGG + chr8_1:60920239-60920258 None:intergenic 30.0%
TAAATAAATAAAGGGGAGGA+AGG + chr8_1:60920286-60920305 None:intergenic 30.0%
GGTATAAATAAATAAAGGGG+AGG + chr8_1:60920290-60920309 None:intergenic 30.0%
! TTAGGAATAATGGAATGGAA+TGG + chr8_1:60920338-60920357 None:intergenic 30.0%
TGAAAGTGGTTTAGGAATAA+TGG + chr8_1:60920348-60920367 None:intergenic 30.0%
GAATAGAATGAAAGTGGTTT+AGG + chr8_1:60920356-60920375 None:intergenic 30.0%
AAGAGAGAATAGAATGAAAG+TGG + chr8_1:60920362-60920381 None:intergenic 30.0%
AGACCAGGTAATTATTTAAC+CGG - chr8_1:60920627-60920646 Msa1204960:CDS 30.0%
CAGAAAATCCTCATATGAAA+AGG + chr8_1:60921262-60921281 None:intergenic 30.0%
TTTGCTTACAATACTCATTG+TGG - chr8_1:60921327-60921346 Msa1204960:five_prime_UTR 30.0%
TTGTGGTGAAAAGAGTAAAA+CGG - chr8_1:60921344-60921363 Msa1204960:five_prime_UTR 30.0%
!! AGTAAAACGGTTTTGTAGTT+CGG - chr8_1:60921357-60921376 Msa1204960:five_prime_UTR 30.0%
! AGTTAAGGTTAGTTGCAAGT+GGG + chr8_1:60920224-60920243 None:intergenic 35.0%
ATGCGAATTGAAGTGATGTT+GGG + chr8_1:60920312-60920331 None:intergenic 35.0%
AATGCGAATTGAAGTGATGT+TGG + chr8_1:60920313-60920332 None:intergenic 35.0%
! GTGGTTTAGGAATAATGGAA+TGG + chr8_1:60920343-60920362 None:intergenic 35.0%
!! TTTTACGACCTTGTTTGCAA+TGG - chr8_1:60920502-60920521 Msa1204960:CDS 35.0%
! GTTTTCGTAGCCAAATTCTT+TGG - chr8_1:60920558-60920577 Msa1204960:CDS 35.0%
AAATAATTACCTGGTCTCTG+TGG + chr8_1:60920624-60920643 None:intergenic 35.0%
AGACCGGTTAAATAATTACC+TGG + chr8_1:60920633-60920652 None:intergenic 35.0%
TCTAGTAATAAGGTGTGTTC+TGG - chr8_1:60921023-60921042 Msa1204960:intron 35.0%
ACTGCCTTCTGTTGAAAAAT+TGG + chr8_1:60921119-60921138 None:intergenic 35.0%
!! AATTTTTCAACAGAAGGCAG+TGG - chr8_1:60921118-60921137 Msa1204960:CDS 35.0%
! GTTCTCAACCTTTTCATATG+AGG - chr8_1:60921251-60921270 Msa1204960:CDS 35.0%
AGCAAAACAGAGAAACGTTA+CGG + chr8_1:60921313-60921332 None:intergenic 35.0%
! TTCTCTAGAGAGAAAGATGA+TGG - chr8_1:60921409-60921428 Msa1204960:five_prime_UTR 35.0%
ATTTGATATTCCACGGTTAC+AGG + chr8_1:60921494-60921513 None:intergenic 35.0%
! AGTTAAGGTTAGTTGCAAGT+GGG + chr8_1:60920224-60920243 None:intergenic 35.0%
ATGCGAATTGAAGTGATGTT+GGG + chr8_1:60920312-60920331 None:intergenic 35.0%
AATGCGAATTGAAGTGATGT+TGG + chr8_1:60920313-60920332 None:intergenic 35.0%
! GTGGTTTAGGAATAATGGAA+TGG + chr8_1:60920343-60920362 None:intergenic 35.0%
!! TTTTACGACCTTGTTTGCAA+TGG - chr8_1:60920502-60920521 Msa1204960:CDS 35.0%
! GTTTTCGTAGCCAAATTCTT+TGG - chr8_1:60920558-60920577 Msa1204960:CDS 35.0%
AAATAATTACCTGGTCTCTG+TGG + chr8_1:60920624-60920643 None:intergenic 35.0%
AGACCGGTTAAATAATTACC+TGG + chr8_1:60920633-60920652 None:intergenic 35.0%
TCTAGTAATAAGGTGTGTTC+TGG - chr8_1:60921023-60921042 Msa1204960:intron 35.0%
ACTGCCTTCTGTTGAAAAAT+TGG + chr8_1:60921119-60921138 None:intergenic 35.0%
!! AATTTTTCAACAGAAGGCAG+TGG - chr8_1:60921118-60921137 Msa1204960:CDS 35.0%
! GTTCTCAACCTTTTCATATG+AGG - chr8_1:60921251-60921270 Msa1204960:CDS 35.0%
AGCAAAACAGAGAAACGTTA+CGG + chr8_1:60921313-60921332 None:intergenic 35.0%
! TTCTCTAGAGAGAAAGATGA+TGG - chr8_1:60921409-60921428 Msa1204960:five_prime_UTR 35.0%
ATTTGATATTCCACGGTTAC+AGG + chr8_1:60921494-60921513 None:intergenic 35.0%
! TTAAGGTTAGTTGCAAGTGG+GGG + chr8_1:60920222-60920241 None:intergenic 40.0%
! GTTAAGGTTAGTTGCAAGTG+GGG + chr8_1:60920223-60920242 None:intergenic 40.0%
! GAGTTAAGGTTAGTTGCAAG+TGG + chr8_1:60920225-60920244 None:intergenic 40.0%
!! GTGTGGTTGGTAGTAAACTA+TGG + chr8_1:60920250-60920269 None:intergenic 40.0%
TGCGAATTGAAGTGATGTTG+GGG + chr8_1:60920311-60920330 None:intergenic 40.0%
GTTTCGATCCATTGCAAACA+AGG + chr8_1:60920513-60920532 None:intergenic 40.0%
!! ATATTTACGGACTCTGAGAC+CGG + chr8_1:60920649-60920668 None:intergenic 40.0%
AAGATTCGCGATCCGAATTT+CGG - chr8_1:60920984-60921003 Msa1204960:intron 40.0%
GAGCTCACGATCTAGTAATA+AGG - chr8_1:60921013-60921032 Msa1204960:intron 40.0%
AAATCAAGATTAATCGCCGC+TGG + chr8_1:60921086-60921105 None:intergenic 40.0%
!! GACGCCAATTTTTCAACAGA+AGG - chr8_1:60921112-60921131 Msa1204960:intron 40.0%
TAGAAACTGGGATTGGAGAT+CGG - chr8_1:60921204-60921223 Msa1204960:CDS 40.0%
! GGTGAATTTTCGTGAGTTAC+CGG - chr8_1:60921378-60921397 Msa1204960:five_prime_UTR 40.0%
! TCTCTAGAGAAATCCTGAAC+CGG + chr8_1:60921400-60921419 None:intergenic 40.0%
! AGAGAAAGATGATGGTTCGT+CGG - chr8_1:60921417-60921436 Msa1204960:five_prime_UTR 40.0%
! TTAAGGTTAGTTGCAAGTGG+GGG + chr8_1:60920222-60920241 None:intergenic 40.0%
! GTTAAGGTTAGTTGCAAGTG+GGG + chr8_1:60920223-60920242 None:intergenic 40.0%
! GAGTTAAGGTTAGTTGCAAG+TGG + chr8_1:60920225-60920244 None:intergenic 40.0%
!! GTGTGGTTGGTAGTAAACTA+TGG + chr8_1:60920250-60920269 None:intergenic 40.0%
TGCGAATTGAAGTGATGTTG+GGG + chr8_1:60920311-60920330 None:intergenic 40.0%
GTTTCGATCCATTGCAAACA+AGG + chr8_1:60920513-60920532 None:intergenic 40.0%
!! ATATTTACGGACTCTGAGAC+CGG + chr8_1:60920649-60920668 None:intergenic 40.0%
AAGATTCGCGATCCGAATTT+CGG - chr8_1:60920984-60921003 Msa1204960:intron 40.0%
GAGCTCACGATCTAGTAATA+AGG - chr8_1:60921013-60921032 Msa1204960:intron 40.0%
AAATCAAGATTAATCGCCGC+TGG + chr8_1:60921086-60921105 None:intergenic 40.0%
!! GACGCCAATTTTTCAACAGA+AGG - chr8_1:60921112-60921131 Msa1204960:intron 40.0%
TAGAAACTGGGATTGGAGAT+CGG - chr8_1:60921204-60921223 Msa1204960:CDS 40.0%
! GGTGAATTTTCGTGAGTTAC+CGG - chr8_1:60921378-60921397 Msa1204960:five_prime_UTR 40.0%
! TCTCTAGAGAAATCCTGAAC+CGG + chr8_1:60921400-60921419 None:intergenic 40.0%
! AGAGAAAGATGATGGTTCGT+CGG - chr8_1:60921417-60921436 Msa1204960:five_prime_UTR 40.0%
TGCAACCCTTTCGAAGAACT+CGG + chr8_1:60920470-60920489 None:intergenic 45.0%
ATCGAAACTCCAGAAGCTCA+AGG - chr8_1:60920525-60920544 Msa1204960:CDS 45.0%
ACCAGCTCGACCAAAGAATT+TGG + chr8_1:60920571-60920590 None:intergenic 45.0%
ATTACCTGGTCTCTGTGGTT+CGG + chr8_1:60920619-60920638 None:intergenic 45.0%
! TCTCTACTGTTTGAAGCGTG+TGG - chr8_1:60920765-60920784 Msa1204960:CDS 45.0%
! GTTTGAAGCGTGTGGAAGAA+CGG - chr8_1:60920773-60920792 Msa1204960:CDS 45.0%
AGAACGGTTAACCCTGTGAA+CGG - chr8_1:60920789-60920808 Msa1204960:CDS 45.0%
! GACTTCTTTGGACTGGAAAC+TGG - chr8_1:60920820-60920839 Msa1204960:CDS 45.0%
!! AGAAGTGTCAGCTCCGATAA+AGG + chr8_1:60920897-60920916 None:intergenic 45.0%
TTTATCGGAGCTGACACTTC+TGG - chr8_1:60920896-60920915 Msa1204960:CDS 45.0%
AGATTCGCGATCCGAATTTC+GGG - chr8_1:60920985-60921004 Msa1204960:intron 45.0%
! TTTTCAACAGAAGGCAGTGG+AGG - chr8_1:60921121-60921140 Msa1204960:CDS 45.0%
GCGTGTTTAGAAACTGGGAT+TGG - chr8_1:60921197-60921216 Msa1204960:CDS 45.0%
!!! TTTTCGTGAGTTACCGGTTC+AGG - chr8_1:60921384-60921403 Msa1204960:five_prime_UTR 45.0%
GATGTGTAAACCTGTAACCG+TGG - chr8_1:60921481-60921500 Msa1204960:five_prime_UTR 45.0%
TGCAACCCTTTCGAAGAACT+CGG + chr8_1:60920470-60920489 None:intergenic 45.0%
ATCGAAACTCCAGAAGCTCA+AGG - chr8_1:60920525-60920544 Msa1204960:CDS 45.0%
ACCAGCTCGACCAAAGAATT+TGG + chr8_1:60920571-60920590 None:intergenic 45.0%
ATTACCTGGTCTCTGTGGTT+CGG + chr8_1:60920619-60920638 None:intergenic 45.0%
! TCTCTACTGTTTGAAGCGTG+TGG - chr8_1:60920765-60920784 Msa1204960:CDS 45.0%
! GTTTGAAGCGTGTGGAAGAA+CGG - chr8_1:60920773-60920792 Msa1204960:CDS 45.0%
AGAACGGTTAACCCTGTGAA+CGG - chr8_1:60920789-60920808 Msa1204960:CDS 45.0%
! GACTTCTTTGGACTGGAAAC+TGG - chr8_1:60920820-60920839 Msa1204960:CDS 45.0%
!! AGAAGTGTCAGCTCCGATAA+AGG + chr8_1:60920897-60920916 None:intergenic 45.0%
TTTATCGGAGCTGACACTTC+TGG - chr8_1:60920896-60920915 Msa1204960:CDS 45.0%
AGATTCGCGATCCGAATTTC+GGG - chr8_1:60920985-60921004 Msa1204960:intron 45.0%
! TTTTCAACAGAAGGCAGTGG+AGG - chr8_1:60921121-60921140 Msa1204960:CDS 45.0%
GCGTGTTTAGAAACTGGGAT+TGG - chr8_1:60921197-60921216 Msa1204960:CDS 45.0%
!!! TTTTCGTGAGTTACCGGTTC+AGG - chr8_1:60921384-60921403 Msa1204960:five_prime_UTR 45.0%
GATGTGTAAACCTGTAACCG+TGG - chr8_1:60921481-60921500 Msa1204960:five_prime_UTR 45.0%
! AGAAGAAGAGGAGGTGTGGT+TGG + chr8_1:60920263-60920282 None:intergenic 50.0%
AAGGAGAAGAAGAGGAGGTG+TGG + chr8_1:60920267-60920286 None:intergenic 50.0%
TGGCTACGAAAACAGTAGCG+TGG + chr8_1:60920551-60920570 None:intergenic 50.0%
GCCAAATTCTTTGGTCGAGC+TGG - chr8_1:60920567-60920586 Msa1204960:CDS 50.0%
TTCGGGGACGTTGGAAATGA+AGG + chr8_1:60920601-60920620 None:intergenic 50.0%
TTACCTGGTCTCTGTGGTTC+GGG + chr8_1:60920618-60920637 None:intergenic 50.0%
!! ACGGTGCTGTTGGACTTCTT+TGG - chr8_1:60920808-60920827 Msa1204960:CDS 50.0%
GCTGTTGGACTTCTTTGGAC+TGG - chr8_1:60920813-60920832 Msa1204960:CDS 50.0%
TGGCACGCTTAGGCCTTTAT+CGG - chr8_1:60920881-60920900 Msa1204960:CDS 50.0%
TGCCACTGATGAAGCTTCTG+AGG - chr8_1:60920932-60920951 Msa1204960:CDS 50.0%
! CACTGATGAAGCTTCTGAGG+CGG - chr8_1:60920935-60920954 Msa1204960:CDS 50.0%
!! AGGTGTGTTCTGGAAGCACA+CGG - chr8_1:60921033-60921052 Msa1204960:intron 50.0%
GAGATCGGAGGAGTTTGTGA+AGG - chr8_1:60921058-60921077 Msa1204960:intron 50.0%
GGAGTTTGTGAAGGTTCCAG+CGG - chr8_1:60921067-60921086 Msa1204960:intron 50.0%
ACGACGGCGTGTTTAGAAAC+TGG - chr8_1:60921191-60921210 Msa1204960:CDS 50.0%
CGACGGCGTGTTTAGAAACT+GGG - chr8_1:60921192-60921211 Msa1204960:CDS 50.0%
AAACTGGGATTGGAGATCGG+TGG - chr8_1:60921207-60921226 Msa1204960:CDS 50.0%
ATTGGAGATCGGTGGAGTCA+CGG - chr8_1:60921215-60921234 Msa1204960:CDS 50.0%
! AGAAGAAGAGGAGGTGTGGT+TGG + chr8_1:60920263-60920282 None:intergenic 50.0%
AAGGAGAAGAAGAGGAGGTG+TGG + chr8_1:60920267-60920286 None:intergenic 50.0%
TGGCTACGAAAACAGTAGCG+TGG + chr8_1:60920551-60920570 None:intergenic 50.0%
GCCAAATTCTTTGGTCGAGC+TGG - chr8_1:60920567-60920586 Msa1204960:CDS 50.0%
TTCGGGGACGTTGGAAATGA+AGG + chr8_1:60920601-60920620 None:intergenic 50.0%
TTACCTGGTCTCTGTGGTTC+GGG + chr8_1:60920618-60920637 None:intergenic 50.0%
!! ACGGTGCTGTTGGACTTCTT+TGG - chr8_1:60920808-60920827 Msa1204960:CDS 50.0%
GCTGTTGGACTTCTTTGGAC+TGG - chr8_1:60920813-60920832 Msa1204960:CDS 50.0%
TGGCACGCTTAGGCCTTTAT+CGG - chr8_1:60920881-60920900 Msa1204960:CDS 50.0%
TGCCACTGATGAAGCTTCTG+AGG - chr8_1:60920932-60920951 Msa1204960:CDS 50.0%
! CACTGATGAAGCTTCTGAGG+CGG - chr8_1:60920935-60920954 Msa1204960:CDS 50.0%
!! AGGTGTGTTCTGGAAGCACA+CGG - chr8_1:60921033-60921052 Msa1204960:intron 50.0%
GAGATCGGAGGAGTTTGTGA+AGG - chr8_1:60921058-60921077 Msa1204960:intron 50.0%
GGAGTTTGTGAAGGTTCCAG+CGG - chr8_1:60921067-60921086 Msa1204960:intron 50.0%
ACGACGGCGTGTTTAGAAAC+TGG - chr8_1:60921191-60921210 Msa1204960:CDS 50.0%
CGACGGCGTGTTTAGAAACT+GGG - chr8_1:60921192-60921211 Msa1204960:CDS 50.0%
AAACTGGGATTGGAGATCGG+TGG - chr8_1:60921207-60921226 Msa1204960:CDS 50.0%
ATTGGAGATCGGTGGAGTCA+CGG - chr8_1:60921215-60921234 Msa1204960:CDS 50.0%
GGAGGAAGGAGAAGAAGAGG+AGG + chr8_1:60920272-60920291 None:intergenic 55.0%
AGGGGAGGAAGGAGAAGAAG+AGG + chr8_1:60920275-60920294 None:intergenic 55.0%
!! TGGGTGCCGAGTTCTTCGAA+AGG - chr8_1:60920461-60920480 Msa1204960:exon 55.0%
!! GGGTGCCGAGTTCTTCGAAA+GGG - chr8_1:60920462-60920481 Msa1204960:exon 55.0%
TACCTGGTCTCTGTGGTTCG+GGG + chr8_1:60920617-60920636 None:intergenic 55.0%
!! AACCCTGTGAACGGTGCTGT+TGG - chr8_1:60920798-60920817 Msa1204960:CDS 55.0%
GTCCAACAGCACCGTTCACA+GGG + chr8_1:60920803-60920822 None:intergenic 55.0%
AGTCCAACAGCACCGTTCAC+AGG + chr8_1:60920804-60920823 None:intergenic 55.0%
GCGGTTGAGACAGTTCTTCG+CGG - chr8_1:60920858-60920877 Msa1204960:CDS 55.0%
GTTGAGACAGTTCTTCGCGG+TGG - chr8_1:60920861-60920880 Msa1204960:CDS 55.0%
GCTTCATCAGTGGCAGTGAG+AGG + chr8_1:60920927-60920946 None:intergenic 55.0%
CGCCTCAGAAGCTTCATCAG+TGG + chr8_1:60920937-60920956 None:intergenic 55.0%
!! GAAGCTTCTGAGGCGGAAGA+CGG - chr8_1:60920942-60920961 Msa1204960:CDS 55.0%
!! ACGGTGGCGCTGATGCTATT+TGG - chr8_1:60920961-60920980 Msa1204960:CDS 55.0%
!! TGTGTTCTGGAAGCACACGG+AGG - chr8_1:60921036-60921055 Msa1204960:intron 55.0%
! TGGAAGCACACGGAGGAGAT+CGG - chr8_1:60921043-60921062 Msa1204960:intron 55.0%
TGGTTCACCGTCGATGACGT+CGG - chr8_1:60921154-60921173 Msa1204960:CDS 55.0%
GATTCCTCCGACGTCATCGA+CGG + chr8_1:60921164-60921183 None:intergenic 55.0%
GTCGATGACGTCGGAGGAAT+CGG - chr8_1:60921163-60921182 Msa1204960:CDS 55.0%
GGAGGAAGGAGAAGAAGAGG+AGG + chr8_1:60920272-60920291 None:intergenic 55.0%
AGGGGAGGAAGGAGAAGAAG+AGG + chr8_1:60920275-60920294 None:intergenic 55.0%
!! TGGGTGCCGAGTTCTTCGAA+AGG - chr8_1:60920461-60920480 Msa1204960:exon 55.0%
!! GGGTGCCGAGTTCTTCGAAA+GGG - chr8_1:60920462-60920481 Msa1204960:exon 55.0%
TACCTGGTCTCTGTGGTTCG+GGG + chr8_1:60920617-60920636 None:intergenic 55.0%
!! AACCCTGTGAACGGTGCTGT+TGG - chr8_1:60920798-60920817 Msa1204960:CDS 55.0%
GTCCAACAGCACCGTTCACA+GGG + chr8_1:60920803-60920822 None:intergenic 55.0%
AGTCCAACAGCACCGTTCAC+AGG + chr8_1:60920804-60920823 None:intergenic 55.0%
GCGGTTGAGACAGTTCTTCG+CGG - chr8_1:60920858-60920877 Msa1204960:CDS 55.0%
GTTGAGACAGTTCTTCGCGG+TGG - chr8_1:60920861-60920880 Msa1204960:CDS 55.0%
GCTTCATCAGTGGCAGTGAG+AGG + chr8_1:60920927-60920946 None:intergenic 55.0%
CGCCTCAGAAGCTTCATCAG+TGG + chr8_1:60920937-60920956 None:intergenic 55.0%
!! GAAGCTTCTGAGGCGGAAGA+CGG - chr8_1:60920942-60920961 Msa1204960:CDS 55.0%
!! ACGGTGGCGCTGATGCTATT+TGG - chr8_1:60920961-60920980 Msa1204960:CDS 55.0%
!! TGTGTTCTGGAAGCACACGG+AGG - chr8_1:60921036-60921055 Msa1204960:intron 55.0%
! TGGAAGCACACGGAGGAGAT+CGG - chr8_1:60921043-60921062 Msa1204960:intron 55.0%
TGGTTCACCGTCGATGACGT+CGG - chr8_1:60921154-60921173 Msa1204960:CDS 55.0%
GATTCCTCCGACGTCATCGA+CGG + chr8_1:60921164-60921183 None:intergenic 55.0%
GTCGATGACGTCGGAGGAAT+CGG - chr8_1:60921163-60921182 Msa1204960:CDS 55.0%
AGTGGGGGAGAGAGACGTGA+AGG + chr8_1:60920207-60920226 None:intergenic 60.0%
! GTAGCGTGGCCTTGAGCTTC+TGG + chr8_1:60920537-60920556 None:intergenic 60.0%
! TCTCTGTGGTTCGGGGACGT+TGG + chr8_1:60920610-60920629 None:intergenic 60.0%
CTGGCACGTGTGTCAAGCAG+CGG - chr8_1:60920839-60920858 Msa1204960:CDS 60.0%
TTCTTCGCGGTGGCACGCTT+AGG - chr8_1:60920871-60920890 Msa1204960:CDS 60.0%
TCGCGATCCGAATTTCGGGC+TGG - chr8_1:60920989-60921008 Msa1204960:intron 60.0%
GAGCTCGCCAGCCCGAAATT+CGG + chr8_1:60920999-60921018 None:intergenic 60.0%
AAGCACACGGAGGAGATCGG+AGG - chr8_1:60921046-60921065 Msa1204960:intron 60.0%
AGAAGGCAGTGGAGGAACGC+CGG - chr8_1:60921129-60921148 Msa1204960:CDS 60.0%
TCATCGACGGTGAACCAGGC+CGG + chr8_1:60921151-60921170 None:intergenic 60.0%
GACGTCATCGACGGTGAACC+AGG + chr8_1:60921155-60921174 None:intergenic 60.0%
TTCACCGTCGATGACGTCGG+AGG - chr8_1:60921157-60921176 Msa1204960:CDS 60.0%
GGAGGAATCGGTGACGACGA+CGG - chr8_1:60921175-60921194 Msa1204960:CDS 60.0%
GGTGGAGTCACGGTGGTGAT+CGG - chr8_1:60921225-60921244 Msa1204960:CDS 60.0%
AGTGGGGGAGAGAGACGTGA+AGG + chr8_1:60920207-60920226 None:intergenic 60.0%
! GTAGCGTGGCCTTGAGCTTC+TGG + chr8_1:60920537-60920556 None:intergenic 60.0%
! TCTCTGTGGTTCGGGGACGT+TGG + chr8_1:60920610-60920629 None:intergenic 60.0%
CTGGCACGTGTGTCAAGCAG+CGG - chr8_1:60920839-60920858 Msa1204960:CDS 60.0%
TTCTTCGCGGTGGCACGCTT+AGG - chr8_1:60920871-60920890 Msa1204960:CDS 60.0%
TCGCGATCCGAATTTCGGGC+TGG - chr8_1:60920989-60921008 Msa1204960:intron 60.0%
GAGCTCGCCAGCCCGAAATT+CGG + chr8_1:60920999-60921018 None:intergenic 60.0%
AAGCACACGGAGGAGATCGG+AGG - chr8_1:60921046-60921065 Msa1204960:intron 60.0%
AGAAGGCAGTGGAGGAACGC+CGG - chr8_1:60921129-60921148 Msa1204960:CDS 60.0%
TCATCGACGGTGAACCAGGC+CGG + chr8_1:60921151-60921170 None:intergenic 60.0%
GACGTCATCGACGGTGAACC+AGG + chr8_1:60921155-60921174 None:intergenic 60.0%
TTCACCGTCGATGACGTCGG+AGG - chr8_1:60921157-60921176 Msa1204960:CDS 60.0%
GGAGGAATCGGTGACGACGA+CGG - chr8_1:60921175-60921194 Msa1204960:CDS 60.0%
GGTGGAGTCACGGTGGTGAT+CGG - chr8_1:60921225-60921244 Msa1204960:CDS 60.0%
GTCCCCGAACCACAGAGACC+AGG - chr8_1:60920612-60920631 Msa1204960:CDS 65.0%
!! GCTTCTGAGGCGGAAGACGG+TGG - chr8_1:60920945-60920964 Msa1204960:CDS 65.0%
GGAGATCGGTGGAGTCACGG+TGG - chr8_1:60921218-60921237 Msa1204960:CDS 65.0%
GTCCCCGAACCACAGAGACC+AGG - chr8_1:60920612-60920631 Msa1204960:CDS 65.0%
!! GCTTCTGAGGCGGAAGACGG+TGG - chr8_1:60920945-60920964 Msa1204960:CDS 65.0%
GGAGATCGGTGGAGTCACGG+TGG - chr8_1:60921218-60921237 Msa1204960:CDS 65.0%
GCAGTGGAGGAACGCCGGCC+TGG - chr8_1:60921134-60921153 Msa1204960:CDS 75.0%
GCAGTGGAGGAACGCCGGCC+TGG - chr8_1:60921134-60921153 Msa1204960:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 60920197 60921550 60920197 ID=Msa1204960;Name=Msa1204960
chr8_1 mRNA 60920197 60921550 60920197 ID=Msa1204960-mRNA-1;Parent=Msa1204960;Name=Msa1204960-mRNA-1;_AED=0.04;_eAED=0.04;_QI=253|1|1|1|1|1|2|279|233
chr8_1 exon 60920197 60920993 60920197 ID=Msa1204960-mRNA-1:exon:19795;Parent=Msa1204960-mRNA-1
chr8_1 exon 60921114 60921550 60921114 ID=Msa1204960-mRNA-1:exon:19794;Parent=Msa1204960-mRNA-1
chr8_1 five_prime_UTR 60921298 60921550 60921298 ID=Msa1204960-mRNA-1:five_prime_utr;Parent=Msa1204960-mRNA-1
chr8_1 CDS 60921114 60921297 60921114 ID=Msa1204960-mRNA-1:cds;Parent=Msa1204960-mRNA-1
chr8_1 CDS 60920476 60920993 60920476 ID=Msa1204960-mRNA-1:cds;Parent=Msa1204960-mRNA-1
chr8_1 three_prime_UTR 60920197 60920475 60920197 ID=Msa1204960-mRNA-1:three_prime_utr;Parent=Msa1204960-mRNA-1
Gene Sequence

>Msa1204960

ATGAGTTGCAATGGGTGCCGAGTTCTTCGAAAGGGTTGCAGCGAGTCTTGTATTTTACGACCTTGTTTGCAATGGATCGAAACTCCAGAAGCTCAAGGCCACGCTACTGTTTTCGTAGCCAAATTCTTTGGTCGAGCTGGTCTTATGTCCTTCATTTCCAACGTCCCCGAACCACAGAGACCAGCTCTGTTTCAGTCTCTACTGTTTGAAGCGTGTGGAAGAACGGTTAACCCTGTGAACGGTGCTGTTGGACTTCTTTGGACTGGAAACTGGCACGTGTGTCAAGCAGCGGTTGAGACAGTTCTTCGCGGTGGCACGCTTAGGCCTTTATCGGAGCTGACACTTCTGGACGCACCTCTCACTGCCACTGATGAAGCTTCTGAGGCGGAAGACGGTGGCGCTGATGCTATTTGGAAGATTCGCGATCCGAATTTCGGGCTGGCGAGCTCACGATCTAGTAATAAGGTGTGTTCTGGAAGCACACGGAGGAGATCGGAGGAGTTTGTGAAGGTTCCAGCGGCGATTAATCTTGATTTACGATTGACGCCAATTTTTCAACAGAAGGCAGTGGAGGAACGCCGGCCTGGTTCACCGTCGATGACGTCGGAGGAATCGGTGACGACGACGGCGTGTTTAGAAACTGGGATTGGAGATCGGTGGAGTCACGGTGGTGATCGGAAAGTTCTCAACCTTTTCATATGA
Protein sequence

>Msa1204960

MSCNGCRVLRKGCSESCILRPCLQWIETPEAQGHATVFVAKFFGRAGLMSFISNVPEPQRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHVCQAAVETVLRGGTLRPLSELTLLDAPLTATDEASEAEDGGADAIWKIRDPNFGLASSRSSNKVCSGSTRRRSEEFVKVPAAINLDLRLTPIFQQKAVEERRPGSPSMTSEESVTTTACLETGIGDRWSHGGDRKVLNLFI*