AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1256580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1256580 MtrunA17_Chr4g0053911 97.854 233 5 0 1 233 1 233 1.47e-169 466
Msa1256580 MtrunA17_Chr5g0401401 70.588 238 58 5 1 233 1 231 1.72e-110 317
Msa1256580 MtrunA17_Chr7g0215281 61.417 127 46 1 1 124 3 129 7.00e-55 177
Msa1256580 MtrunA17_Chr6g0457431 55.319 141 51 1 1 141 3 131 1.21e-52 167
Msa1256580 MtrunA17_Chr1g0207471 62.791 129 44 1 1 129 1 125 1.85e-51 165
Msa1256580 MtrunA17_Chr6g0469271 60.550 109 41 1 1 107 3 111 1.07e-46 154
Msa1256580 MtrunA17_Chr6g0470651 50.000 120 56 2 1 118 3 120 1.48e-41 141
Msa1256580 MtrunA17_Chr6g0470631 47.581 124 61 2 1 122 3 124 1.85e-40 138
Msa1256580 MtrunA17_Chr1g0209841 48.387 124 50 3 1 122 3 114 1.52e-37 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1256580 AT4G37540.1 57.831 249 80 8 1 233 1 240 1.85e-82 246
Msa1256580 AT3G49940.1 57.143 252 82 7 1 232 1 246 6.56e-82 245
Msa1256580 AT5G67420.2 53.937 254 92 7 1 233 1 250 8.04e-75 227
Msa1256580 AT5G67420.1 53.937 254 92 7 1 233 1 250 8.04e-75 227
Msa1256580 AT1G67100.1 66.667 111 37 0 1 111 3 113 1.02e-53 173
Msa1256580 AT3G02550.1 49.718 177 73 4 1 173 3 167 5.98e-52 169
Msa1256580 AT1G68510.1 63.462 104 38 0 1 104 3 106 4.76e-48 158

Find 103 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACTCATGTTTCTATTT+TGG 0.155235 8_2:-56645973 None:intergenic
AAGATTCGCGATCCGAATTT+CGG 0.249148 8_2:+56646520 Msa1256580:CDS
GTAGCGTGGCCTTGAGCTTC+TGG 0.259138 8_2:-56646070 None:intergenic
GTTGGGGTATAAATAAATAA+AGG 0.296968 8_2:-56645833 None:intergenic
GAATAGAAAGAAAGTGGTTT+AGG 0.300622 8_2:-56645889 None:intergenic
TTACCTGGTCTATGTGGTTC+AGG 0.302101 8_2:-56646151 None:intergenic
ACCAGCTCGACCAAAGAATT+TGG 0.305659 8_2:-56646104 None:intergenic
GACTTCTTTGGACTGGAAAC+TGG 0.306937 8_2:+56646356 Msa1256580:CDS
ATTTGATATTCCACGGTTAC+AGG 0.313506 8_2:-56647031 None:intergenic
ACGGTGCTGTTGGACTTCTT+TGG 0.315165 8_2:+56646344 Msa1256580:CDS
AGAAAGTGGTTTAGGAATAA+TGG 0.315956 8_2:-56645881 None:intergenic
TTTATCGGAGCTGACACTTC+TGG 0.333923 8_2:+56646432 Msa1256580:CDS
AGATTCGCGATCCGAATTTC+GGG 0.347093 8_2:+56646521 Msa1256580:CDS
ACGGTGGCGCTGATGCTATT+TGG 0.350213 8_2:+56646497 Msa1256580:CDS
ACGACGGCGTGTTTAGAAAC+TGG 0.362464 8_2:+56646727 Msa1256580:CDS
TCTAGTAATAAGGTGTGTTC+TGG 0.372758 8_2:+56646559 Msa1256580:CDS
TGGCACGCTTAGGCCTTTAT+CGG 0.389750 8_2:+56646417 Msa1256580:CDS
GAGCTCGCCAGCCCGAAATT+CGG 0.420698 8_2:-56646532 None:intergenic
TTGGGGTATAAATAAATAAA+GGG 0.424281 8_2:-56645832 None:intergenic
GAGCTCACGATCTAGTAATA+AGG 0.427686 8_2:+56646549 Msa1256580:CDS
AAGAGAGAATAGAAAGAAAG+TGG 0.431602 8_2:-56645895 None:intergenic
AGTAAACTATGGTTGAGTTA+AGG 0.435665 8_2:-56645777 None:intergenic
GTCGATGACGTCGGAGGAAT+CGG 0.439790 8_2:+56646699 Msa1256580:CDS
AGAAGTGTCAGCTCCGATAA+AGG 0.449767 8_2:-56646430 None:intergenic
CAGAAAATCCTCATATGAAA+AGG 0.454062 8_2:-56646795 None:intergenic
AGAAGAAGAGGAGGTGTGGT+TGG 0.462946 8_2:-56645801 None:intergenic
GCCAAATTCTTTGGTCGAGC+TGG 0.464513 8_2:+56646103 Msa1256580:CDS
GCTGTTGGACTTCTTTGGAC+TGG 0.482667 8_2:+56646349 Msa1256580:CDS
GACGTCATCGACGGTGAACC+AGG 0.485410 8_2:-56646688 None:intergenic
ATGCGAATTGAAGTGATGTT+GGG 0.486771 8_2:-56645850 None:intergenic
GTGTGGTTGGTAGTAAACTA+TGG 0.487162 8_2:-56645788 None:intergenic
TTCTCTAGAGAGAAAGATGA+TGG 0.491224 8_2:+56646945 Msa1256580:three_prime_UTR
AGGGGAGGAAGGAGAAGAAG+AGG 0.493231 8_2:-56645813 None:intergenic
AATAGAAACATGAGTTGCAA+TGG 0.497518 8_2:+56645977 Msa1256580:exon
TTGTGGTGAAAAGAGTAAAA+CGG 0.498145 8_2:+56646880 Msa1256580:three_prime_UTR
GCAGTGGAGGAACGCCGGCC+TGG 0.500212 8_2:+56646670 Msa1256580:CDS
AATGCGAATTGAAGTGATGT+TGG 0.500278 8_2:-56645851 None:intergenic
TCTCTAGAGAAATCCTGAAC+CGG 0.501718 8_2:-56646933 None:intergenic
TACCTGGTCTATGTGGTTCA+GGG 0.504621 8_2:-56646150 None:intergenic
CGACGGCGTGTTTAGAAACT+GGG 0.509803 8_2:+56646728 Msa1256580:CDS
AGTCCAACAGCACCGTTCAC+AGG 0.514245 8_2:-56646337 None:intergenic
AATTAATTACCTGGTCTATG+TGG 0.516471 8_2:-56646157 None:intergenic
GTTTCGATCCATTGCAAGCA+AGG 0.521828 8_2:-56646046 None:intergenic
TAAATAAATAAAGGGGAGGA+AGG 0.524788 8_2:-56645824 None:intergenic
ATTTAATATTTGATATTCCA+CGG 0.529132 8_2:-56647038 None:intergenic
ATAGAAACATGAGTTGCAAT+GGG 0.529336 8_2:+56645978 Msa1256580:exon
AAGGAGAAGAAGAGGAGGTG+TGG 0.529924 8_2:-56645805 None:intergenic
TTCAGGGACGTTAGAAATGA+AGG 0.532305 8_2:-56646134 None:intergenic
AAATCAAGATTAATCGCCGC+TGG 0.534004 8_2:-56646619 None:intergenic
GGTGGAGTCACGGTGGTGAT+CGG 0.543236 8_2:+56646761 Msa1256580:CDS
GGAGGAAGGAGAAGAAGAGG+AGG 0.543266 8_2:-56645810 None:intergenic
TAGAAACTGGGATTGGAGAT+CGG 0.545733 8_2:+56646740 Msa1256580:CDS
TAAGGTTAGTTGCAAGCAAG+TGG 0.549359 8_2:-56645759 None:intergenic
ATTGGAGATCGGTGGAGTCA+CGG 0.549508 8_2:+56646751 Msa1256580:CDS
AACCCTGTGAACGGTGCTGT+TGG 0.557626 8_2:+56646334 Msa1256580:CDS
GCGTGTTTAGAAACTGGGAT+TGG 0.557756 8_2:+56646733 Msa1256580:CDS
AGCAAAACAGAGAAACGTTA+CGG 0.559764 8_2:-56646846 None:intergenic
TTCTTCGCGGTGGCACGCTT+AGG 0.561098 8_2:+56646407 Msa1256580:CDS
AAGGTTAGTTGCAAGCAAGT+GGG 0.561944 8_2:-56645758 None:intergenic
GGGTGCCGAGTTCTTCGAAA+GGG 0.563138 8_2:+56645998 Msa1256580:CDS
GTTTGAAGCGTGTGGAAGAA+CGG 0.563405 8_2:+56646309 Msa1256580:CDS
TCGCGATCCGAATTTCGGGC+TGG 0.563771 8_2:+56646525 Msa1256580:CDS
TGGAAGCAAACGGAGGAGAT+CGG 0.569877 8_2:+56646579 Msa1256580:CDS
TGCCACTGATGAAGCTTCTG+AGG 0.578795 8_2:+56646468 Msa1256580:CDS
GTGGTTTAGGAATAATGGAA+TGG 0.579027 8_2:-56645876 None:intergenic
TCATCGACGGTGAACCAGGC+CGG 0.580860 8_2:-56646684 None:intergenic
AGGTGTGTTCTGGAAGCAAA+CGG 0.583068 8_2:+56646569 Msa1256580:CDS
AGAAGGCAGTGGAGGAACGC+CGG 0.585660 8_2:+56646665 Msa1256580:CDS
GAAGCTTCTGAGGCGGAAGA+CGG 0.587046 8_2:+56646478 Msa1256580:CDS
TTTGATATTCCACGGTTACA+GGG 0.588797 8_2:-56647030 None:intergenic
CACTGATGAAGCTTCTGAGG+CGG 0.596477 8_2:+56646471 Msa1256580:CDS
AAGTGGGGAGAGAGACGTGA+AGG 0.599979 8_2:-56645742 None:intergenic
TCTCTACTGTTTGAAGCGTG+TGG 0.600037 8_2:+56646301 Msa1256580:CDS
GTCCAACAGCACCGTTCACA+GGG 0.600320 8_2:-56646336 None:intergenic
AGAGAAAGATGATGGTTCGT+CGG 0.604350 8_2:+56646953 Msa1256580:three_prime_UTR
GCTTCATCAGTGGCAGTGAG+AGG 0.608069 8_2:-56646460 None:intergenic
TTTGCTTACAATACTCATTG+TGG 0.609288 8_2:+56646863 Msa1256580:three_prime_UTR
AGGTTAGTTGCAAGCAAGTG+GGG 0.609783 8_2:-56645757 None:intergenic
CGCCTCAGAAGCTTCATCAG+TGG 0.613409 8_2:-56646470 None:intergenic
GAGATCGGAGGAGTTTGTGA+AGG 0.623682 8_2:+56646594 Msa1256580:CDS
GCGGTTGAGACAGTTCTTCG+CGG 0.626359 8_2:+56646394 Msa1256580:CDS
GCTTCTGAGGCGGAAGACGG+TGG 0.641101 8_2:+56646481 Msa1256580:CDS
TGCAACCCTTTCGAAGAACT+CGG 0.644072 8_2:-56646003 None:intergenic
TGCGAATTGAAGTGATGTTG+GGG 0.644823 8_2:-56645849 None:intergenic
GATTCCTCCGACGTCATCGA+CGG 0.648497 8_2:-56646697 None:intergenic
TGGGTGCCGAGTTCTTCGAA+AGG 0.651709 8_2:+56645997 Msa1256580:CDS
TGGGGTATAAATAAATAAAG+GGG 0.654291 8_2:-56645831 None:intergenic
GTCCCTGAACCACATAGACC+AGG 0.654563 8_2:+56646148 Msa1256580:CDS
TGGTTCACCGTCGATGACGT+CGG 0.655686 8_2:+56646690 Msa1256580:CDS
AAGCAAACGGAGGAGATCGG+AGG 0.657755 8_2:+56646582 Msa1256580:CDS
GGAGGAATCGGTGACGACGA+CGG 0.660492 8_2:+56646711 Msa1256580:CDS
CTGGCACGTGTGTCAAGCAG+CGG 0.661568 8_2:+56646375 Msa1256580:CDS
AGAACGGTTAACCCTGTGAA+CGG 0.663812 8_2:+56646325 Msa1256580:CDS
GGAGTTTGTGAAGGTTCCAG+CGG 0.666158 8_2:+56646603 Msa1256580:CDS
GTTGAGACAGTTCTTCGCGG+TGG 0.669818 8_2:+56646397 Msa1256580:CDS
GGTATAAATAAATAAAGGGG+AGG 0.672012 8_2:-56645828 None:intergenic
GATGTGTAACCCTGTAACCG+TGG 0.679105 8_2:+56647021 Msa1256580:three_prime_UTR
ATCGAAACTCCAGAAGCTCA+AGG 0.686071 8_2:+56646061 Msa1256580:CDS
AAACTGGGATTGGAGATCGG+TGG 0.696279 8_2:+56646743 Msa1256580:CDS
TTCACCGTCGATGACGTCGG+AGG 0.699966 8_2:+56646693 Msa1256580:CDS
TGGCTACGAAAACAGTAGCG+TGG 0.700795 8_2:-56646084 None:intergenic
TGTGTTCTGGAAGCAAACGG+AGG 0.716901 8_2:+56646572 Msa1256580:CDS
GGAGATCGGTGGAGTCACGG+TGG 0.745894 8_2:+56646754 Msa1256580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATATTTGATATTCCA+CGG - chr8_2:56647041-56647060 None:intergenic 15.0%
!! ATTTAATATTTGATATTCCA+CGG - chr8_2:56647041-56647060 None:intergenic 15.0%
!! TTGGGGTATAAATAAATAAA+GGG - chr8_2:56645835-56645854 None:intergenic 20.0%
!! CAAACTTTCTCTTATATTTA+CGG - chr8_2:56646198-56646217 None:intergenic 20.0%
!! TTGGGGTATAAATAAATAAA+GGG - chr8_2:56645835-56645854 None:intergenic 20.0%
!! CAAACTTTCTCTTATATTTA+CGG - chr8_2:56646198-56646217 None:intergenic 20.0%
! TGGGGTATAAATAAATAAAG+GGG - chr8_2:56645834-56645853 None:intergenic 25.0%
! GTTGGGGTATAAATAAATAA+AGG - chr8_2:56645836-56645855 None:intergenic 25.0%
! CGTAAATATAAGAGAAAGTT+TGG + chr8_2:56646196-56646215 Msa1256580:intron 25.0%
! TGGGGTATAAATAAATAAAG+GGG - chr8_2:56645834-56645853 None:intergenic 25.0%
! GTTGGGGTATAAATAAATAA+AGG - chr8_2:56645836-56645855 None:intergenic 25.0%
! CGTAAATATAAGAGAAAGTT+TGG + chr8_2:56646196-56646215 Msa1256580:intron 25.0%
AGTAAACTATGGTTGAGTTA+AGG - chr8_2:56645780-56645799 None:intergenic 30.0%
TAAATAAATAAAGGGGAGGA+AGG - chr8_2:56645827-56645846 None:intergenic 30.0%
GGTATAAATAAATAAAGGGG+AGG - chr8_2:56645831-56645850 None:intergenic 30.0%
AGAAAGTGGTTTAGGAATAA+TGG - chr8_2:56645884-56645903 None:intergenic 30.0%
GAATAGAAAGAAAGTGGTTT+AGG - chr8_2:56645892-56645911 None:intergenic 30.0%
AAGAGAGAATAGAAAGAAAG+TGG - chr8_2:56645898-56645917 None:intergenic 30.0%
!! TGCAACTCATGTTTCTATTT+TGG - chr8_2:56645976-56645995 None:intergenic 30.0%
AATAGAAACATGAGTTGCAA+TGG + chr8_2:56645977-56645996 Msa1256580:exon 30.0%
ATAGAAACATGAGTTGCAAT+GGG + chr8_2:56645978-56645997 Msa1256580:exon 30.0%
AATTAATTACCTGGTCTATG+TGG - chr8_2:56646160-56646179 None:intergenic 30.0%
AGACCAGGTAATTAATTAAC+CGG + chr8_2:56646163-56646182 Msa1256580:intron 30.0%
CAGAAAATCCTCATATGAAA+AGG - chr8_2:56646798-56646817 None:intergenic 30.0%
TTTGCTTACAATACTCATTG+TGG + chr8_2:56646863-56646882 Msa1256580:three_prime_UTR 30.0%
TTGTGGTGAAAAGAGTAAAA+CGG + chr8_2:56646880-56646899 Msa1256580:three_prime_UTR 30.0%
!! AGTAAAACGGTTTTGTAGTT+CGG + chr8_2:56646893-56646912 Msa1256580:three_prime_UTR 30.0%
AGTAAACTATGGTTGAGTTA+AGG - chr8_2:56645780-56645799 None:intergenic 30.0%
TAAATAAATAAAGGGGAGGA+AGG - chr8_2:56645827-56645846 None:intergenic 30.0%
GGTATAAATAAATAAAGGGG+AGG - chr8_2:56645831-56645850 None:intergenic 30.0%
AGAAAGTGGTTTAGGAATAA+TGG - chr8_2:56645884-56645903 None:intergenic 30.0%
GAATAGAAAGAAAGTGGTTT+AGG - chr8_2:56645892-56645911 None:intergenic 30.0%
AAGAGAGAATAGAAAGAAAG+TGG - chr8_2:56645898-56645917 None:intergenic 30.0%
!! TGCAACTCATGTTTCTATTT+TGG - chr8_2:56645976-56645995 None:intergenic 30.0%
AATAGAAACATGAGTTGCAA+TGG + chr8_2:56645977-56645996 Msa1256580:exon 30.0%
ATAGAAACATGAGTTGCAAT+GGG + chr8_2:56645978-56645997 Msa1256580:exon 30.0%
AATTAATTACCTGGTCTATG+TGG - chr8_2:56646160-56646179 None:intergenic 30.0%
AGACCAGGTAATTAATTAAC+CGG + chr8_2:56646163-56646182 Msa1256580:intron 30.0%
CAGAAAATCCTCATATGAAA+AGG - chr8_2:56646798-56646817 None:intergenic 30.0%
TTTGCTTACAATACTCATTG+TGG + chr8_2:56646863-56646882 Msa1256580:three_prime_UTR 30.0%
TTGTGGTGAAAAGAGTAAAA+CGG + chr8_2:56646880-56646899 Msa1256580:three_prime_UTR 30.0%
!! AGTAAAACGGTTTTGTAGTT+CGG + chr8_2:56646893-56646912 Msa1256580:three_prime_UTR 30.0%
ATGCGAATTGAAGTGATGTT+GGG - chr8_2:56645853-56645872 None:intergenic 35.0%
AATGCGAATTGAAGTGATGT+TGG - chr8_2:56645854-56645873 None:intergenic 35.0%
! GTGGTTTAGGAATAATGGAA+TGG - chr8_2:56645879-56645898 None:intergenic 35.0%
! GTTTTCGTAGCCAAATTCTT+TGG + chr8_2:56646094-56646113 Msa1256580:CDS 35.0%
AGACCGGTTAATTAATTACC+TGG - chr8_2:56646169-56646188 None:intergenic 35.0%
!! ATATTTACGGACTCTAAGAC+CGG - chr8_2:56646185-56646204 None:intergenic 35.0%
TCTAGTAATAAGGTGTGTTC+TGG + chr8_2:56646559-56646578 Msa1256580:CDS 35.0%
ACTGCCTTCTGTTGAAAAAT+TGG - chr8_2:56646655-56646674 None:intergenic 35.0%
!! AATTTTTCAACAGAAGGCAG+TGG + chr8_2:56646654-56646673 Msa1256580:CDS 35.0%
! GTTCTCAACCTTTTCATATG+AGG + chr8_2:56646787-56646806 Msa1256580:CDS 35.0%
AGCAAAACAGAGAAACGTTA+CGG - chr8_2:56646849-56646868 None:intergenic 35.0%
! TTCTCTAGAGAGAAAGATGA+TGG + chr8_2:56646945-56646964 Msa1256580:three_prime_UTR 35.0%
TTTGATATTCCACGGTTACA+GGG - chr8_2:56647033-56647052 None:intergenic 35.0%
ATTTGATATTCCACGGTTAC+AGG - chr8_2:56647034-56647053 None:intergenic 35.0%
ATGCGAATTGAAGTGATGTT+GGG - chr8_2:56645853-56645872 None:intergenic 35.0%
AATGCGAATTGAAGTGATGT+TGG - chr8_2:56645854-56645873 None:intergenic 35.0%
! GTGGTTTAGGAATAATGGAA+TGG - chr8_2:56645879-56645898 None:intergenic 35.0%
! GTTTTCGTAGCCAAATTCTT+TGG + chr8_2:56646094-56646113 Msa1256580:CDS 35.0%
AGACCGGTTAATTAATTACC+TGG - chr8_2:56646169-56646188 None:intergenic 35.0%
!! ATATTTACGGACTCTAAGAC+CGG - chr8_2:56646185-56646204 None:intergenic 35.0%
TCTAGTAATAAGGTGTGTTC+TGG + chr8_2:56646559-56646578 Msa1256580:CDS 35.0%
ACTGCCTTCTGTTGAAAAAT+TGG - chr8_2:56646655-56646674 None:intergenic 35.0%
!! AATTTTTCAACAGAAGGCAG+TGG + chr8_2:56646654-56646673 Msa1256580:CDS 35.0%
! GTTCTCAACCTTTTCATATG+AGG + chr8_2:56646787-56646806 Msa1256580:CDS 35.0%
AGCAAAACAGAGAAACGTTA+CGG - chr8_2:56646849-56646868 None:intergenic 35.0%
! TTCTCTAGAGAGAAAGATGA+TGG + chr8_2:56646945-56646964 Msa1256580:three_prime_UTR 35.0%
TTTGATATTCCACGGTTACA+GGG - chr8_2:56647033-56647052 None:intergenic 35.0%
ATTTGATATTCCACGGTTAC+AGG - chr8_2:56647034-56647053 None:intergenic 35.0%
! AAGGTTAGTTGCAAGCAAGT+GGG - chr8_2:56645761-56645780 None:intergenic 40.0%
! TAAGGTTAGTTGCAAGCAAG+TGG - chr8_2:56645762-56645781 None:intergenic 40.0%
!! GTGTGGTTGGTAGTAAACTA+TGG - chr8_2:56645791-56645810 None:intergenic 40.0%
TGCGAATTGAAGTGATGTTG+GGG - chr8_2:56645852-56645871 None:intergenic 40.0%
! TTTTACGACCTTGCTTGCAA+TGG + chr8_2:56646038-56646057 Msa1256580:CDS 40.0%
TTCAGGGACGTTAGAAATGA+AGG - chr8_2:56646137-56646156 None:intergenic 40.0%
AAGATTCGCGATCCGAATTT+CGG + chr8_2:56646520-56646539 Msa1256580:CDS 40.0%
GAGCTCACGATCTAGTAATA+AGG + chr8_2:56646549-56646568 Msa1256580:CDS 40.0%
AAATCAAGATTAATCGCCGC+TGG - chr8_2:56646622-56646641 None:intergenic 40.0%
!! GACGCCAATTTTTCAACAGA+AGG + chr8_2:56646648-56646667 Msa1256580:CDS 40.0%
TAGAAACTGGGATTGGAGAT+CGG + chr8_2:56646740-56646759 Msa1256580:CDS 40.0%
! GGTGAATTTTCGTGAGTTAC+CGG + chr8_2:56646914-56646933 Msa1256580:three_prime_UTR 40.0%
! TCTCTAGAGAAATCCTGAAC+CGG - chr8_2:56646936-56646955 None:intergenic 40.0%
! AGAGAAAGATGATGGTTCGT+CGG + chr8_2:56646953-56646972 Msa1256580:three_prime_UTR 40.0%
! AAGGTTAGTTGCAAGCAAGT+GGG - chr8_2:56645761-56645780 None:intergenic 40.0%
! TAAGGTTAGTTGCAAGCAAG+TGG - chr8_2:56645762-56645781 None:intergenic 40.0%
!! GTGTGGTTGGTAGTAAACTA+TGG - chr8_2:56645791-56645810 None:intergenic 40.0%
TGCGAATTGAAGTGATGTTG+GGG - chr8_2:56645852-56645871 None:intergenic 40.0%
! TTTTACGACCTTGCTTGCAA+TGG + chr8_2:56646038-56646057 Msa1256580:CDS 40.0%
TTCAGGGACGTTAGAAATGA+AGG - chr8_2:56646137-56646156 None:intergenic 40.0%
AAGATTCGCGATCCGAATTT+CGG + chr8_2:56646520-56646539 Msa1256580:CDS 40.0%
GAGCTCACGATCTAGTAATA+AGG + chr8_2:56646549-56646568 Msa1256580:CDS 40.0%
AAATCAAGATTAATCGCCGC+TGG - chr8_2:56646622-56646641 None:intergenic 40.0%
!! GACGCCAATTTTTCAACAGA+AGG + chr8_2:56646648-56646667 Msa1256580:CDS 40.0%
TAGAAACTGGGATTGGAGAT+CGG + chr8_2:56646740-56646759 Msa1256580:CDS 40.0%
! GGTGAATTTTCGTGAGTTAC+CGG + chr8_2:56646914-56646933 Msa1256580:three_prime_UTR 40.0%
! TCTCTAGAGAAATCCTGAAC+CGG - chr8_2:56646936-56646955 None:intergenic 40.0%
! AGAGAAAGATGATGGTTCGT+CGG + chr8_2:56646953-56646972 Msa1256580:three_prime_UTR 40.0%
! AGGTTAGTTGCAAGCAAGTG+GGG - chr8_2:56645760-56645779 None:intergenic 45.0%
TGCAACCCTTTCGAAGAACT+CGG - chr8_2:56646006-56646025 None:intergenic 45.0%
GTTTCGATCCATTGCAAGCA+AGG - chr8_2:56646049-56646068 None:intergenic 45.0%
ATCGAAACTCCAGAAGCTCA+AGG + chr8_2:56646061-56646080 Msa1256580:CDS 45.0%
ACCAGCTCGACCAAAGAATT+TGG - chr8_2:56646107-56646126 None:intergenic 45.0%
TACCTGGTCTATGTGGTTCA+GGG - chr8_2:56646153-56646172 None:intergenic 45.0%
TTACCTGGTCTATGTGGTTC+AGG - chr8_2:56646154-56646173 None:intergenic 45.0%
! TCTCTACTGTTTGAAGCGTG+TGG + chr8_2:56646301-56646320 Msa1256580:CDS 45.0%
! GTTTGAAGCGTGTGGAAGAA+CGG + chr8_2:56646309-56646328 Msa1256580:CDS 45.0%
AGAACGGTTAACCCTGTGAA+CGG + chr8_2:56646325-56646344 Msa1256580:CDS 45.0%
! GACTTCTTTGGACTGGAAAC+TGG + chr8_2:56646356-56646375 Msa1256580:CDS 45.0%
!! AGAAGTGTCAGCTCCGATAA+AGG - chr8_2:56646433-56646452 None:intergenic 45.0%
TTTATCGGAGCTGACACTTC+TGG + chr8_2:56646432-56646451 Msa1256580:CDS 45.0%
AGATTCGCGATCCGAATTTC+GGG + chr8_2:56646521-56646540 Msa1256580:CDS 45.0%
!! AGGTGTGTTCTGGAAGCAAA+CGG + chr8_2:56646569-56646588 Msa1256580:CDS 45.0%
! TTTTCAACAGAAGGCAGTGG+AGG + chr8_2:56646657-56646676 Msa1256580:CDS 45.0%
GCGTGTTTAGAAACTGGGAT+TGG + chr8_2:56646733-56646752 Msa1256580:CDS 45.0%
!!! TTTTCGTGAGTTACCGGTTC+AGG + chr8_2:56646920-56646939 Msa1256580:three_prime_UTR 45.0%
! AGGTTAGTTGCAAGCAAGTG+GGG - chr8_2:56645760-56645779 None:intergenic 45.0%
TGCAACCCTTTCGAAGAACT+CGG - chr8_2:56646006-56646025 None:intergenic 45.0%
GTTTCGATCCATTGCAAGCA+AGG - chr8_2:56646049-56646068 None:intergenic 45.0%
ATCGAAACTCCAGAAGCTCA+AGG + chr8_2:56646061-56646080 Msa1256580:CDS 45.0%
ACCAGCTCGACCAAAGAATT+TGG - chr8_2:56646107-56646126 None:intergenic 45.0%
TACCTGGTCTATGTGGTTCA+GGG - chr8_2:56646153-56646172 None:intergenic 45.0%
TTACCTGGTCTATGTGGTTC+AGG - chr8_2:56646154-56646173 None:intergenic 45.0%
! TCTCTACTGTTTGAAGCGTG+TGG + chr8_2:56646301-56646320 Msa1256580:CDS 45.0%
! GTTTGAAGCGTGTGGAAGAA+CGG + chr8_2:56646309-56646328 Msa1256580:CDS 45.0%
AGAACGGTTAACCCTGTGAA+CGG + chr8_2:56646325-56646344 Msa1256580:CDS 45.0%
! GACTTCTTTGGACTGGAAAC+TGG + chr8_2:56646356-56646375 Msa1256580:CDS 45.0%
!! AGAAGTGTCAGCTCCGATAA+AGG - chr8_2:56646433-56646452 None:intergenic 45.0%
TTTATCGGAGCTGACACTTC+TGG + chr8_2:56646432-56646451 Msa1256580:CDS 45.0%
AGATTCGCGATCCGAATTTC+GGG + chr8_2:56646521-56646540 Msa1256580:CDS 45.0%
!! AGGTGTGTTCTGGAAGCAAA+CGG + chr8_2:56646569-56646588 Msa1256580:CDS 45.0%
! TTTTCAACAGAAGGCAGTGG+AGG + chr8_2:56646657-56646676 Msa1256580:CDS 45.0%
GCGTGTTTAGAAACTGGGAT+TGG + chr8_2:56646733-56646752 Msa1256580:CDS 45.0%
!!! TTTTCGTGAGTTACCGGTTC+AGG + chr8_2:56646920-56646939 Msa1256580:three_prime_UTR 45.0%
! AGAAGAAGAGGAGGTGTGGT+TGG - chr8_2:56645804-56645823 None:intergenic 50.0%
AAGGAGAAGAAGAGGAGGTG+TGG - chr8_2:56645808-56645827 None:intergenic 50.0%
TGGCTACGAAAACAGTAGCG+TGG - chr8_2:56646087-56646106 None:intergenic 50.0%
GCCAAATTCTTTGGTCGAGC+TGG + chr8_2:56646103-56646122 Msa1256580:CDS 50.0%
!! ACGGTGCTGTTGGACTTCTT+TGG + chr8_2:56646344-56646363 Msa1256580:CDS 50.0%
GCTGTTGGACTTCTTTGGAC+TGG + chr8_2:56646349-56646368 Msa1256580:CDS 50.0%
TGGCACGCTTAGGCCTTTAT+CGG + chr8_2:56646417-56646436 Msa1256580:CDS 50.0%
TGCCACTGATGAAGCTTCTG+AGG + chr8_2:56646468-56646487 Msa1256580:CDS 50.0%
! CACTGATGAAGCTTCTGAGG+CGG + chr8_2:56646471-56646490 Msa1256580:CDS 50.0%
!! TGTGTTCTGGAAGCAAACGG+AGG + chr8_2:56646572-56646591 Msa1256580:CDS 50.0%
TGGAAGCAAACGGAGGAGAT+CGG + chr8_2:56646579-56646598 Msa1256580:CDS 50.0%
GAGATCGGAGGAGTTTGTGA+AGG + chr8_2:56646594-56646613 Msa1256580:CDS 50.0%
GGAGTTTGTGAAGGTTCCAG+CGG + chr8_2:56646603-56646622 Msa1256580:CDS 50.0%
ACGACGGCGTGTTTAGAAAC+TGG + chr8_2:56646727-56646746 Msa1256580:CDS 50.0%
CGACGGCGTGTTTAGAAACT+GGG + chr8_2:56646728-56646747 Msa1256580:CDS 50.0%
AAACTGGGATTGGAGATCGG+TGG + chr8_2:56646743-56646762 Msa1256580:CDS 50.0%
ATTGGAGATCGGTGGAGTCA+CGG + chr8_2:56646751-56646770 Msa1256580:CDS 50.0%
GATGTGTAACCCTGTAACCG+TGG + chr8_2:56647021-56647040 Msa1256580:three_prime_UTR 50.0%
! AGAAGAAGAGGAGGTGTGGT+TGG - chr8_2:56645804-56645823 None:intergenic 50.0%
AAGGAGAAGAAGAGGAGGTG+TGG - chr8_2:56645808-56645827 None:intergenic 50.0%
TGGCTACGAAAACAGTAGCG+TGG - chr8_2:56646087-56646106 None:intergenic 50.0%
GCCAAATTCTTTGGTCGAGC+TGG + chr8_2:56646103-56646122 Msa1256580:CDS 50.0%
!! ACGGTGCTGTTGGACTTCTT+TGG + chr8_2:56646344-56646363 Msa1256580:CDS 50.0%
GCTGTTGGACTTCTTTGGAC+TGG + chr8_2:56646349-56646368 Msa1256580:CDS 50.0%
TGGCACGCTTAGGCCTTTAT+CGG + chr8_2:56646417-56646436 Msa1256580:CDS 50.0%
TGCCACTGATGAAGCTTCTG+AGG + chr8_2:56646468-56646487 Msa1256580:CDS 50.0%
! CACTGATGAAGCTTCTGAGG+CGG + chr8_2:56646471-56646490 Msa1256580:CDS 50.0%
!! TGTGTTCTGGAAGCAAACGG+AGG + chr8_2:56646572-56646591 Msa1256580:CDS 50.0%
TGGAAGCAAACGGAGGAGAT+CGG + chr8_2:56646579-56646598 Msa1256580:CDS 50.0%
GAGATCGGAGGAGTTTGTGA+AGG + chr8_2:56646594-56646613 Msa1256580:CDS 50.0%
GGAGTTTGTGAAGGTTCCAG+CGG + chr8_2:56646603-56646622 Msa1256580:CDS 50.0%
ACGACGGCGTGTTTAGAAAC+TGG + chr8_2:56646727-56646746 Msa1256580:CDS 50.0%
CGACGGCGTGTTTAGAAACT+GGG + chr8_2:56646728-56646747 Msa1256580:CDS 50.0%
AAACTGGGATTGGAGATCGG+TGG + chr8_2:56646743-56646762 Msa1256580:CDS 50.0%
ATTGGAGATCGGTGGAGTCA+CGG + chr8_2:56646751-56646770 Msa1256580:CDS 50.0%
GATGTGTAACCCTGTAACCG+TGG + chr8_2:56647021-56647040 Msa1256580:three_prime_UTR 50.0%
AAGTGGGGAGAGAGACGTGA+AGG - chr8_2:56645745-56645764 None:intergenic 55.0%
GGAGGAAGGAGAAGAAGAGG+AGG - chr8_2:56645813-56645832 None:intergenic 55.0%
AGGGGAGGAAGGAGAAGAAG+AGG - chr8_2:56645816-56645835 None:intergenic 55.0%
!! TGGGTGCCGAGTTCTTCGAA+AGG + chr8_2:56645997-56646016 Msa1256580:CDS 55.0%
!! GGGTGCCGAGTTCTTCGAAA+GGG + chr8_2:56645998-56646017 Msa1256580:CDS 55.0%
GTCCCTGAACCACATAGACC+AGG + chr8_2:56646148-56646167 Msa1256580:CDS 55.0%
!! AACCCTGTGAACGGTGCTGT+TGG + chr8_2:56646334-56646353 Msa1256580:CDS 55.0%
GTCCAACAGCACCGTTCACA+GGG - chr8_2:56646339-56646358 None:intergenic 55.0%
AGTCCAACAGCACCGTTCAC+AGG - chr8_2:56646340-56646359 None:intergenic 55.0%
GCGGTTGAGACAGTTCTTCG+CGG + chr8_2:56646394-56646413 Msa1256580:CDS 55.0%
GTTGAGACAGTTCTTCGCGG+TGG + chr8_2:56646397-56646416 Msa1256580:CDS 55.0%
GCTTCATCAGTGGCAGTGAG+AGG - chr8_2:56646463-56646482 None:intergenic 55.0%
CGCCTCAGAAGCTTCATCAG+TGG - chr8_2:56646473-56646492 None:intergenic 55.0%
!! GAAGCTTCTGAGGCGGAAGA+CGG + chr8_2:56646478-56646497 Msa1256580:CDS 55.0%
!! ACGGTGGCGCTGATGCTATT+TGG + chr8_2:56646497-56646516 Msa1256580:CDS 55.0%
AAGCAAACGGAGGAGATCGG+AGG + chr8_2:56646582-56646601 Msa1256580:CDS 55.0%
TGGTTCACCGTCGATGACGT+CGG + chr8_2:56646690-56646709 Msa1256580:CDS 55.0%
GATTCCTCCGACGTCATCGA+CGG - chr8_2:56646700-56646719 None:intergenic 55.0%
GTCGATGACGTCGGAGGAAT+CGG + chr8_2:56646699-56646718 Msa1256580:CDS 55.0%
AAGTGGGGAGAGAGACGTGA+AGG - chr8_2:56645745-56645764 None:intergenic 55.0%
GGAGGAAGGAGAAGAAGAGG+AGG - chr8_2:56645813-56645832 None:intergenic 55.0%
AGGGGAGGAAGGAGAAGAAG+AGG - chr8_2:56645816-56645835 None:intergenic 55.0%
!! TGGGTGCCGAGTTCTTCGAA+AGG + chr8_2:56645997-56646016 Msa1256580:CDS 55.0%
!! GGGTGCCGAGTTCTTCGAAA+GGG + chr8_2:56645998-56646017 Msa1256580:CDS 55.0%
GTCCCTGAACCACATAGACC+AGG + chr8_2:56646148-56646167 Msa1256580:CDS 55.0%
!! AACCCTGTGAACGGTGCTGT+TGG + chr8_2:56646334-56646353 Msa1256580:CDS 55.0%
GTCCAACAGCACCGTTCACA+GGG - chr8_2:56646339-56646358 None:intergenic 55.0%
AGTCCAACAGCACCGTTCAC+AGG - chr8_2:56646340-56646359 None:intergenic 55.0%
GCGGTTGAGACAGTTCTTCG+CGG + chr8_2:56646394-56646413 Msa1256580:CDS 55.0%
GTTGAGACAGTTCTTCGCGG+TGG + chr8_2:56646397-56646416 Msa1256580:CDS 55.0%
GCTTCATCAGTGGCAGTGAG+AGG - chr8_2:56646463-56646482 None:intergenic 55.0%
CGCCTCAGAAGCTTCATCAG+TGG - chr8_2:56646473-56646492 None:intergenic 55.0%
!! GAAGCTTCTGAGGCGGAAGA+CGG + chr8_2:56646478-56646497 Msa1256580:CDS 55.0%
!! ACGGTGGCGCTGATGCTATT+TGG + chr8_2:56646497-56646516 Msa1256580:CDS 55.0%
AAGCAAACGGAGGAGATCGG+AGG + chr8_2:56646582-56646601 Msa1256580:CDS 55.0%
TGGTTCACCGTCGATGACGT+CGG + chr8_2:56646690-56646709 Msa1256580:CDS 55.0%
GATTCCTCCGACGTCATCGA+CGG - chr8_2:56646700-56646719 None:intergenic 55.0%
GTCGATGACGTCGGAGGAAT+CGG + chr8_2:56646699-56646718 Msa1256580:CDS 55.0%
! GTAGCGTGGCCTTGAGCTTC+TGG - chr8_2:56646073-56646092 None:intergenic 60.0%
CTGGCACGTGTGTCAAGCAG+CGG + chr8_2:56646375-56646394 Msa1256580:CDS 60.0%
TTCTTCGCGGTGGCACGCTT+AGG + chr8_2:56646407-56646426 Msa1256580:CDS 60.0%
TCGCGATCCGAATTTCGGGC+TGG + chr8_2:56646525-56646544 Msa1256580:CDS 60.0%
GAGCTCGCCAGCCCGAAATT+CGG - chr8_2:56646535-56646554 None:intergenic 60.0%
AGAAGGCAGTGGAGGAACGC+CGG + chr8_2:56646665-56646684 Msa1256580:CDS 60.0%
TCATCGACGGTGAACCAGGC+CGG - chr8_2:56646687-56646706 None:intergenic 60.0%
GACGTCATCGACGGTGAACC+AGG - chr8_2:56646691-56646710 None:intergenic 60.0%
TTCACCGTCGATGACGTCGG+AGG + chr8_2:56646693-56646712 Msa1256580:CDS 60.0%
GGAGGAATCGGTGACGACGA+CGG + chr8_2:56646711-56646730 Msa1256580:CDS 60.0%
GGTGGAGTCACGGTGGTGAT+CGG + chr8_2:56646761-56646780 Msa1256580:CDS 60.0%
! GTAGCGTGGCCTTGAGCTTC+TGG - chr8_2:56646073-56646092 None:intergenic 60.0%
CTGGCACGTGTGTCAAGCAG+CGG + chr8_2:56646375-56646394 Msa1256580:CDS 60.0%
TTCTTCGCGGTGGCACGCTT+AGG + chr8_2:56646407-56646426 Msa1256580:CDS 60.0%
TCGCGATCCGAATTTCGGGC+TGG + chr8_2:56646525-56646544 Msa1256580:CDS 60.0%
GAGCTCGCCAGCCCGAAATT+CGG - chr8_2:56646535-56646554 None:intergenic 60.0%
AGAAGGCAGTGGAGGAACGC+CGG + chr8_2:56646665-56646684 Msa1256580:CDS 60.0%
TCATCGACGGTGAACCAGGC+CGG - chr8_2:56646687-56646706 None:intergenic 60.0%
GACGTCATCGACGGTGAACC+AGG - chr8_2:56646691-56646710 None:intergenic 60.0%
TTCACCGTCGATGACGTCGG+AGG + chr8_2:56646693-56646712 Msa1256580:CDS 60.0%
GGAGGAATCGGTGACGACGA+CGG + chr8_2:56646711-56646730 Msa1256580:CDS 60.0%
GGTGGAGTCACGGTGGTGAT+CGG + chr8_2:56646761-56646780 Msa1256580:CDS 60.0%
!! GCTTCTGAGGCGGAAGACGG+TGG + chr8_2:56646481-56646500 Msa1256580:CDS 65.0%
GGAGATCGGTGGAGTCACGG+TGG + chr8_2:56646754-56646773 Msa1256580:CDS 65.0%
!! GCTTCTGAGGCGGAAGACGG+TGG + chr8_2:56646481-56646500 Msa1256580:CDS 65.0%
GGAGATCGGTGGAGTCACGG+TGG + chr8_2:56646754-56646773 Msa1256580:CDS 65.0%
GCAGTGGAGGAACGCCGGCC+TGG + chr8_2:56646670-56646689 Msa1256580:CDS 75.0%
GCAGTGGAGGAACGCCGGCC+TGG + chr8_2:56646670-56646689 Msa1256580:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 56645735 56647089 56645735 ID=Msa1256580;Name=Msa1256580
chr8_2 mRNA 56645735 56647089 56645735 ID=Msa1256580-mRNA-1;Parent=Msa1256580;Name=Msa1256580-mRNA-1;_AED=0.04;_eAED=0.04;_QI=251|1|1|1|1|1|2|282|233
chr8_2 exon 56645735 56646169 56645735 ID=Msa1256580-mRNA-1:exon:17450;Parent=Msa1256580-mRNA-1
chr8_2 exon 56646290 56647089 56646290 ID=Msa1256580-mRNA-1:exon:17451;Parent=Msa1256580-mRNA-1
chr8_2 five_prime_UTR 56645735 56645985 56645735 ID=Msa1256580-mRNA-1:five_prime_utr;Parent=Msa1256580-mRNA-1
chr8_2 CDS 56645986 56646169 56645986 ID=Msa1256580-mRNA-1:cds;Parent=Msa1256580-mRNA-1
chr8_2 CDS 56646290 56646807 56646290 ID=Msa1256580-mRNA-1:cds;Parent=Msa1256580-mRNA-1
chr8_2 three_prime_UTR 56646808 56647089 56646808 ID=Msa1256580-mRNA-1:three_prime_utr;Parent=Msa1256580-mRNA-1
Gene Sequence

>Msa1256580

ATGAGTTGCAATGGGTGCCGAGTTCTTCGAAAGGGTTGCAGTGAGTCATGTATTTTACGACCTTGCTTGCAATGGATCGAAACTCCAGAAGCTCAAGGCCACGCTACTGTTTTCGTAGCCAAATTCTTTGGTCGAGCTGGTCTCATGTCCTTCATTTCTAACGTCCCTGAACCACATAGACCAGCTCTGTTTCAGTCTCTACTGTTTGAAGCGTGTGGAAGAACGGTTAACCCTGTGAACGGTGCTGTTGGACTTCTTTGGACTGGAAACTGGCACGTGTGTCAAGCAGCGGTTGAGACAGTTCTTCGCGGTGGCACGCTTAGGCCTTTATCGGAGCTGACACTTCTGGACGCACCTCTCACTGCCACTGATGAAGCTTCTGAGGCGGAAGACGGTGGCGCTGATGCTATTTGGAAGATTCGCGATCCGAATTTCGGGCTGGCGAGCTCACGATCTAGTAATAAGGTGTGTTCTGGAAGCAAACGGAGGAGATCGGAGGAGTTTGTGAAGGTTCCAGCGGCGATTAATCTTGATTTACGATTGACGCCAATTTTTCAACAGAAGGCAGTGGAGGAACGCCGGCCTGGTTCACCGTCGATGACGTCGGAGGAATCGGTGACGACGACGGCGTGTTTAGAAACTGGGATTGGAGATCGGTGGAGTCACGGTGGTGATCGGAAAGTTCTCAACCTTTTCATATGA
Protein sequence

>Msa1256580

MSCNGCRVLRKGCSESCILRPCLQWIETPEAQGHATVFVAKFFGRAGLMSFISNVPEPHRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHVCQAAVETVLRGGTLRPLSELTLLDAPLTATDEASEAEDGGADAIWKIRDPNFGLASSRSSNKVCSGSKRRRSEEFVKVPAAINLDLRLTPIFQQKAVEERRPGSPSMTSEESVTTTACLETGIGDRWSHGGDRKVLNLFI*