AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1329330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329330 MtrunA17_Chr4g0053911 98.283 233 4 0 1 233 1 233 3.00e-170 468
Msa1329330 MtrunA17_Chr5g0401401 71.008 238 57 5 1 233 1 231 2.45e-111 318
Msa1329330 MtrunA17_Chr7g0215281 57.857 140 54 2 1 135 3 142 1.56e-54 177
Msa1329330 MtrunA17_Chr1g0207471 58.042 143 57 2 1 140 1 143 8.93e-54 171
Msa1329330 MtrunA17_Chr6g0457431 55.319 141 51 1 1 141 3 131 8.61e-53 167
Msa1329330 MtrunA17_Chr6g0469271 60.550 109 41 1 1 107 3 111 1.70e-46 154
Msa1329330 MtrunA17_Chr6g0470651 51.667 120 54 3 1 118 3 120 7.59e-42 142
Msa1329330 MtrunA17_Chr6g0470631 49.194 124 59 3 1 122 3 124 1.06e-40 139
Msa1329330 MtrunA17_Chr1g0209841 48.387 124 50 3 1 122 3 114 1.72e-37 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329330 AT3G49940.1 57.937 252 80 7 1 232 1 246 1.32e-83 249
Msa1329330 AT4G37540.1 57.490 247 84 7 1 233 1 240 2.11e-82 246
Msa1329330 AT5G67420.2 54.724 254 90 7 1 233 1 250 8.45e-77 232
Msa1329330 AT5G67420.1 54.724 254 90 7 1 233 1 250 8.45e-77 232
Msa1329330 AT1G67100.1 67.273 110 36 0 1 110 3 112 1.17e-53 172
Msa1329330 AT3G02550.1 49.718 177 73 4 1 173 3 167 2.72e-52 170
Msa1329330 AT1G68510.1 61.947 113 41 1 1 111 3 115 1.13e-48 160

Find 102 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACTCATGTTTCTATTT+TGG 0.155235 8_4:-47843862 None:intergenic
AAGATTCGCGATCCGAATTT+CGG 0.249558 8_4:+47844409 Msa1329330:CDS
TTACCTGGTCTCTGTGGTTC+GGG 0.275915 8_4:-47844040 None:intergenic
GTTGGGGTATAAATAAATAA+AGG 0.296968 8_4:-47843717 None:intergenic
GAATAGAAAGAAAGTGGTTT+AGG 0.300622 8_4:-47843778 None:intergenic
GACTTCTTTGGACTGGAAAC+TGG 0.306937 8_4:+47844245 Msa1329330:CDS
GTAGCGTGGCCTTGAGCTTC+TGG 0.312181 8_4:-47843959 None:intergenic
ATTTGATATTCCACGGTTAC+AGG 0.313336 8_4:-47844922 None:intergenic
ACGGTGCTGTTGGACTTCTT+TGG 0.315165 8_4:+47844233 Msa1329330:CDS
AGAAAGTGGTTTAGGAATAA+TGG 0.315956 8_4:-47843770 None:intergenic
TTTACCGGAGCTGACACTTC+TGG 0.333923 8_4:+47844321 Msa1329330:CDS
AGATTCGCGATCCGAATTTC+GGG 0.342540 8_4:+47844410 Msa1329330:CDS
ACGGTGGCGCTGCTGCTATT+TGG 0.345295 8_4:+47844386 Msa1329330:CDS
ACGACGGCGTGTTTAGAAAC+TGG 0.362464 8_4:+47844616 Msa1329330:CDS
TCTAGTAATAAGGTGTGTTC+TGG 0.372758 8_4:+47844448 Msa1329330:CDS
ATTACCTGGTCTCTGTGGTT+CGG 0.410585 8_4:-47844041 None:intergenic
TTGGGGTATAAATAAATAAA+GGG 0.424281 8_4:-47843716 None:intergenic
GAGCTCACGATCTAGTAATA+AGG 0.427686 8_4:+47844438 Msa1329330:CDS
AAGAGAGAATAGAAAGAAAG+TGG 0.431602 8_4:-47843784 None:intergenic
AGTAAACTATGGTTGAGTTA+AGG 0.435665 8_4:-47843661 None:intergenic
GTCGATGACGTCGGAGGAAT+CGG 0.439790 8_4:+47844588 Msa1329330:CDS
TGGCACGCTTAGGCCTTTAC+CGG 0.448239 8_4:+47844306 Msa1329330:CDS
CAGAAAATCCTCATATGAAA+AGG 0.454062 8_4:-47844684 None:intergenic
AGAAGAAGAGGAGGTGTGGT+TGG 0.462946 8_4:-47843685 None:intergenic
TTCTTCGTGGTGGCACGCTT+AGG 0.479220 8_4:+47844296 Msa1329330:CDS
GCTGTTGGACTTCTTTGGAC+TGG 0.482667 8_4:+47844238 Msa1329330:CDS
GACGTCATCGACGGTGAACC+AGG 0.485410 8_4:-47844577 None:intergenic
GCAAAATTCTTTGGTCGAGC+TGG 0.485866 8_4:+47843992 Msa1329330:CDS
ATGCGAATTGAAGTGATGTT+GGG 0.486771 8_4:-47843734 None:intergenic
GTGTGGTTGGTAGTAAACTA+TGG 0.487162 8_4:-47843672 None:intergenic
AGGGGAGGAAGGAGAAGAAG+AGG 0.493231 8_4:-47843697 None:intergenic
AATAGAAACATGAGTTGCAA+TGG 0.497518 8_4:+47843866 Msa1329330:exon
TTGTGGTGAAAAGAGTAAAA+CGG 0.498145 8_4:+47844769 Msa1329330:three_prime_UTR
AATGCGAATTGAAGTGATGT+TGG 0.500278 8_4:-47843735 None:intergenic
GTTTCGATCCATTGCAAACA+AGG 0.507273 8_4:-47843935 None:intergenic
TCTCTAGAGAAATCCTGAAC+CGG 0.508750 8_4:-47844822 None:intergenic
CGACGGCGTGTTTAGAAACT+GGG 0.509803 8_4:+47844617 Msa1329330:CDS
TTCGGGGACGTTGGAAATGA+AGG 0.510532 8_4:-47844023 None:intergenic
AGTCCAACAGCACCGTTCAC+AGG 0.514245 8_4:-47844226 None:intergenic
GGGTGCCGAGTTCTTCGAAA+AGG 0.515559 8_4:+47843887 Msa1329330:CDS
GCAGTGGAGGAACGACGGCC+TGG 0.518874 8_4:+47844559 Msa1329330:CDS
AGAAGTGTCAGCTCCGGTAA+AGG 0.524390 8_4:-47844319 None:intergenic
TAAATAAATAAAGGGGAGGA+AGG 0.524788 8_4:-47843708 None:intergenic
ATTTAATATTTGATATTCCA+CGG 0.529132 8_4:-47844929 None:intergenic
ATAGAAACATGAGTTGCAAT+GGG 0.529336 8_4:+47843867 Msa1329330:exon
AAGGAGAAGAAGAGGAGGTG+TGG 0.529924 8_4:-47843689 None:intergenic
GGTGGAGTCACGGTGGTGAT+CGG 0.543236 8_4:+47844650 Msa1329330:CDS
GGAGGAAGGAGAAGAAGAGG+AGG 0.543266 8_4:-47843694 None:intergenic
TAGAAACTGGGATTGGAGAT+CGG 0.545733 8_4:+47844629 Msa1329330:CDS
TAAGGTTAGTTGCAAGCAAG+TGG 0.549359 8_4:-47843643 None:intergenic
ATTGGAGATCGGTGGAGTCA+CGG 0.549508 8_4:+47844640 Msa1329330:CDS
AGAGAGTCAGAGAAAGATGA+TGG 0.551093 8_4:+47844840 Msa1329330:three_prime_UTR
AACCCTGTGAACGGTGCTGT+TGG 0.557626 8_4:+47844223 Msa1329330:CDS
GCGTGTTTAGAAACTGGGAT+TGG 0.557756 8_4:+47844622 Msa1329330:CDS
GTGGTTTAGGAATAATGGAA+TGG 0.559576 8_4:-47843765 None:intergenic
AGCAAAACAGAGAAACGTTA+CGG 0.559764 8_4:-47844735 None:intergenic
GCGGTTGAGACGGTTCTTCG+TGG 0.561357 8_4:+47844283 Msa1329330:CDS
AAGGTTAGTTGCAAGCAAGT+GGG 0.561944 8_4:-47843642 None:intergenic
GTTTGAAGCGTGTGGAAGAA+CGG 0.563405 8_4:+47844198 Msa1329330:CDS
TCTCTGTGGTTCGGGGACGT+TGG 0.563762 8_4:-47844032 None:intergenic
TTAGGAATAATGGAATGGAA+TGG 0.568834 8_4:-47843760 None:intergenic
TGGAAGCAAACGGAGGAGAT+CGG 0.569877 8_4:+47844468 Msa1329330:CDS
TGCCACTGATGAAGCTTCTG+AGG 0.578795 8_4:+47844357 Msa1329330:CDS
AAATCAAGATTAATCGCCGC+GGG 0.581245 8_4:-47844508 None:intergenic
AGGTGTGTTCTGGAAGCAAA+CGG 0.583068 8_4:+47844458 Msa1329330:CDS
GAAGCTTCTGAGGCGGAAGA+CGG 0.587046 8_4:+47844367 Msa1329330:CDS
TCGCGATCCGAATTTCGGGT+TGG 0.591044 8_4:+47844414 Msa1329330:CDS
TTGTCAAGCAGCGGTTGAGA+CGG 0.594446 8_4:+47844273 Msa1329330:CDS
CACTGATGAAGCTTCTGAGG+CGG 0.596477 8_4:+47844360 Msa1329330:CDS
AAGTGGGGAGAGAGACGTGA+AGG 0.599979 8_4:-47843626 None:intergenic
TCTCTACTGTTTGAAGCGTG+TGG 0.600037 8_4:+47844190 Msa1329330:CDS
GTCCAACAGCACCGTTCACA+GGG 0.600320 8_4:-47844225 None:intergenic
GATGTGTAAACCTGTAACCG+TGG 0.601531 8_4:+47844912 Msa1329330:three_prime_UTR
GCTTCATCAGTGGCAGTGAG+AGG 0.602040 8_4:-47844349 None:intergenic
TTTGCTTACAATACTCATTG+TGG 0.609288 8_4:+47844752 Msa1329330:three_prime_UTR
AGGTTAGTTGCAAGCAAGTG+GGG 0.609783 8_4:-47843641 None:intergenic
CGCCTCAGAAGCTTCATCAG+TGG 0.613409 8_4:-47844359 None:intergenic
AGAGAAAGATGATGGTTCGT+CGG 0.615967 8_4:+47844848 Msa1329330:three_prime_UTR
GTCCCCGAACCACAGAGACC+AGG 0.617602 8_4:+47844037 Msa1329330:CDS
AAATAATTACCTGGTCTCTG+TGG 0.626571 8_4:-47844046 None:intergenic
CTGGCACGTTTGTCAAGCAG+CGG 0.631827 8_4:+47844264 Msa1329330:CDS
GAGATCGGAGGAGTTTGTCA+AGG 0.633312 8_4:+47844483 Msa1329330:CDS
TACCTGGTCTCTGTGGTTCG+GGG 0.637914 8_4:-47844039 None:intergenic
GCTTCTGAGGCGGAAGACGG+TGG 0.641101 8_4:+47844370 Msa1329330:CDS
TGCGAATTGAAGTGATGTTG+GGG 0.644823 8_4:-47843733 None:intergenic
GTTGAGACGGTTCTTCGTGG+TGG 0.647601 8_4:+47844286 Msa1329330:CDS
GATTCCTCCGACGTCATCGA+CGG 0.648497 8_4:-47844586 None:intergenic
GGAGTTTGTCAAGGTTCCCG+CGG 0.651001 8_4:+47844492 Msa1329330:CDS
TGGGGTATAAATAAATAAAG+GGG 0.654291 8_4:-47843715 None:intergenic
TGGTTCACCGTCGATGACGT+CGG 0.655686 8_4:+47844579 Msa1329330:CDS
AGAAGGCAGTGGAGGAACGA+CGG 0.656112 8_4:+47844554 Msa1329330:CDS
ATCGAAACACCAGAAGCTCA+AGG 0.656960 8_4:+47843950 Msa1329330:CDS
AAGCAAACGGAGGAGATCGG+AGG 0.657755 8_4:+47844471 Msa1329330:CDS
GGAGGAATCGGTGACGACGA+CGG 0.660492 8_4:+47844600 Msa1329330:CDS
AGAACGGTTAACCCTGTGAA+CGG 0.663812 8_4:+47844214 Msa1329330:CDS
TTGCTACGAAAACAGTAGCG+TGG 0.670232 8_4:-47843973 None:intergenic
GGTATAAATAAATAAAGGGG+AGG 0.672012 8_4:-47843712 None:intergenic
AAACTGGGATTGGAGATCGG+TGG 0.696279 8_4:+47844632 Msa1329330:CDS
TAAATCAAGATTAATCGCCG+CGG 0.697247 8_4:-47844509 None:intergenic
TTCACCGTCGATGACGTCGG+AGG 0.699966 8_4:+47844582 Msa1329330:CDS
TGTGTTCTGGAAGCAAACGG+AGG 0.716901 8_4:+47844461 Msa1329330:CDS
GGAGATCGGTGGAGTCACGG+TGG 0.745894 8_4:+47844643 Msa1329330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATATTTGATATTCCA+CGG - chr8_4:47844932-47844951 None:intergenic 15.0%
!! ATTTAATATTTGATATTCCA+CGG - chr8_4:47844932-47844951 None:intergenic 15.0%
!! TTGGGGTATAAATAAATAAA+GGG - chr8_4:47843719-47843738 None:intergenic 20.0%
!! CAAACTTTCTCTTATATTTA+CGG - chr8_4:47844087-47844106 None:intergenic 20.0%
!! TTGGGGTATAAATAAATAAA+GGG - chr8_4:47843719-47843738 None:intergenic 20.0%
!! CAAACTTTCTCTTATATTTA+CGG - chr8_4:47844087-47844106 None:intergenic 20.0%
! TGGGGTATAAATAAATAAAG+GGG - chr8_4:47843718-47843737 None:intergenic 25.0%
! GTTGGGGTATAAATAAATAA+AGG - chr8_4:47843720-47843739 None:intergenic 25.0%
! CGTAAATATAAGAGAAAGTT+TGG + chr8_4:47844085-47844104 Msa1329330:intron 25.0%
! TGGGGTATAAATAAATAAAG+GGG - chr8_4:47843718-47843737 None:intergenic 25.0%
! GTTGGGGTATAAATAAATAA+AGG - chr8_4:47843720-47843739 None:intergenic 25.0%
! CGTAAATATAAGAGAAAGTT+TGG + chr8_4:47844085-47844104 Msa1329330:intron 25.0%
AGTAAACTATGGTTGAGTTA+AGG - chr8_4:47843664-47843683 None:intergenic 30.0%
TAAATAAATAAAGGGGAGGA+AGG - chr8_4:47843711-47843730 None:intergenic 30.0%
GGTATAAATAAATAAAGGGG+AGG - chr8_4:47843715-47843734 None:intergenic 30.0%
! TTAGGAATAATGGAATGGAA+TGG - chr8_4:47843763-47843782 None:intergenic 30.0%
AGAAAGTGGTTTAGGAATAA+TGG - chr8_4:47843773-47843792 None:intergenic 30.0%
GAATAGAAAGAAAGTGGTTT+AGG - chr8_4:47843781-47843800 None:intergenic 30.0%
AAGAGAGAATAGAAAGAAAG+TGG - chr8_4:47843787-47843806 None:intergenic 30.0%
!! TGCAACTCATGTTTCTATTT+TGG - chr8_4:47843865-47843884 None:intergenic 30.0%
AATAGAAACATGAGTTGCAA+TGG + chr8_4:47843866-47843885 Msa1329330:exon 30.0%
ATAGAAACATGAGTTGCAAT+GGG + chr8_4:47843867-47843886 Msa1329330:exon 30.0%
! GTTTTCGTAGCAAAATTCTT+TGG + chr8_4:47843983-47844002 Msa1329330:CDS 30.0%
AGACCAGGTAATTATTTAAC+CGG + chr8_4:47844052-47844071 Msa1329330:intron 30.0%
CAGAAAATCCTCATATGAAA+AGG - chr8_4:47844687-47844706 None:intergenic 30.0%
TTTGCTTACAATACTCATTG+TGG + chr8_4:47844752-47844771 Msa1329330:three_prime_UTR 30.0%
TTGTGGTGAAAAGAGTAAAA+CGG + chr8_4:47844769-47844788 Msa1329330:three_prime_UTR 30.0%
!! AGTAAAACGGTTTTGTAGTT+CGG + chr8_4:47844782-47844801 Msa1329330:three_prime_UTR 30.0%
AGTAAACTATGGTTGAGTTA+AGG - chr8_4:47843664-47843683 None:intergenic 30.0%
TAAATAAATAAAGGGGAGGA+AGG - chr8_4:47843711-47843730 None:intergenic 30.0%
GGTATAAATAAATAAAGGGG+AGG - chr8_4:47843715-47843734 None:intergenic 30.0%
! TTAGGAATAATGGAATGGAA+TGG - chr8_4:47843763-47843782 None:intergenic 30.0%
AGAAAGTGGTTTAGGAATAA+TGG - chr8_4:47843773-47843792 None:intergenic 30.0%
GAATAGAAAGAAAGTGGTTT+AGG - chr8_4:47843781-47843800 None:intergenic 30.0%
AAGAGAGAATAGAAAGAAAG+TGG - chr8_4:47843787-47843806 None:intergenic 30.0%
!! TGCAACTCATGTTTCTATTT+TGG - chr8_4:47843865-47843884 None:intergenic 30.0%
AATAGAAACATGAGTTGCAA+TGG + chr8_4:47843866-47843885 Msa1329330:exon 30.0%
ATAGAAACATGAGTTGCAAT+GGG + chr8_4:47843867-47843886 Msa1329330:exon 30.0%
! GTTTTCGTAGCAAAATTCTT+TGG + chr8_4:47843983-47844002 Msa1329330:CDS 30.0%
AGACCAGGTAATTATTTAAC+CGG + chr8_4:47844052-47844071 Msa1329330:intron 30.0%
CAGAAAATCCTCATATGAAA+AGG - chr8_4:47844687-47844706 None:intergenic 30.0%
TTTGCTTACAATACTCATTG+TGG + chr8_4:47844752-47844771 Msa1329330:three_prime_UTR 30.0%
TTGTGGTGAAAAGAGTAAAA+CGG + chr8_4:47844769-47844788 Msa1329330:three_prime_UTR 30.0%
!! AGTAAAACGGTTTTGTAGTT+CGG + chr8_4:47844782-47844801 Msa1329330:three_prime_UTR 30.0%
ATGCGAATTGAAGTGATGTT+GGG - chr8_4:47843737-47843756 None:intergenic 35.0%
AATGCGAATTGAAGTGATGT+TGG - chr8_4:47843738-47843757 None:intergenic 35.0%
! GTGGTTTAGGAATAATGGAA+TGG - chr8_4:47843768-47843787 None:intergenic 35.0%
!! TTTTACGACCTTGTTTGCAA+TGG + chr8_4:47843927-47843946 Msa1329330:CDS 35.0%
AAATAATTACCTGGTCTCTG+TGG - chr8_4:47844049-47844068 None:intergenic 35.0%
AGACCGGTTAAATAATTACC+TGG - chr8_4:47844058-47844077 None:intergenic 35.0%
TCTAGTAATAAGGTGTGTTC+TGG + chr8_4:47844448-47844467 Msa1329330:CDS 35.0%
TAAATCAAGATTAATCGCCG+CGG - chr8_4:47844512-47844531 None:intergenic 35.0%
ACTGCCTTCTGTTGAAAAAT+CGG - chr8_4:47844544-47844563 None:intergenic 35.0%
! GTTCTCAACCTTTTCATATG+AGG + chr8_4:47844676-47844695 Msa1329330:CDS 35.0%
AGCAAAACAGAGAAACGTTA+CGG - chr8_4:47844738-47844757 None:intergenic 35.0%
ATTTGATATTCCACGGTTAC+AGG - chr8_4:47844925-47844944 None:intergenic 35.0%
ATGCGAATTGAAGTGATGTT+GGG - chr8_4:47843737-47843756 None:intergenic 35.0%
AATGCGAATTGAAGTGATGT+TGG - chr8_4:47843738-47843757 None:intergenic 35.0%
! GTGGTTTAGGAATAATGGAA+TGG - chr8_4:47843768-47843787 None:intergenic 35.0%
!! TTTTACGACCTTGTTTGCAA+TGG + chr8_4:47843927-47843946 Msa1329330:CDS 35.0%
AAATAATTACCTGGTCTCTG+TGG - chr8_4:47844049-47844068 None:intergenic 35.0%
AGACCGGTTAAATAATTACC+TGG - chr8_4:47844058-47844077 None:intergenic 35.0%
TCTAGTAATAAGGTGTGTTC+TGG + chr8_4:47844448-47844467 Msa1329330:CDS 35.0%
TAAATCAAGATTAATCGCCG+CGG - chr8_4:47844512-47844531 None:intergenic 35.0%
ACTGCCTTCTGTTGAAAAAT+CGG - chr8_4:47844544-47844563 None:intergenic 35.0%
! GTTCTCAACCTTTTCATATG+AGG + chr8_4:47844676-47844695 Msa1329330:CDS 35.0%
AGCAAAACAGAGAAACGTTA+CGG - chr8_4:47844738-47844757 None:intergenic 35.0%
ATTTGATATTCCACGGTTAC+AGG - chr8_4:47844925-47844944 None:intergenic 35.0%
! AAGGTTAGTTGCAAGCAAGT+GGG - chr8_4:47843645-47843664 None:intergenic 40.0%
! TAAGGTTAGTTGCAAGCAAG+TGG - chr8_4:47843646-47843665 None:intergenic 40.0%
!! GTGTGGTTGGTAGTAAACTA+TGG - chr8_4:47843675-47843694 None:intergenic 40.0%
TGCGAATTGAAGTGATGTTG+GGG - chr8_4:47843736-47843755 None:intergenic 40.0%
! TGCAACCTTTTCGAAGAACT+CGG - chr8_4:47843895-47843914 None:intergenic 40.0%
GTTTCGATCCATTGCAAACA+AGG - chr8_4:47843938-47843957 None:intergenic 40.0%
!! ATATTTACGGACTCTGAGAC+CGG - chr8_4:47844074-47844093 None:intergenic 40.0%
AAGATTCGCGATCCGAATTT+CGG + chr8_4:47844409-47844428 Msa1329330:CDS 40.0%
GAGCTCACGATCTAGTAATA+AGG + chr8_4:47844438-47844457 Msa1329330:CDS 40.0%
AAATCAAGATTAATCGCCGC+GGG - chr8_4:47844511-47844530 None:intergenic 40.0%
!! GATTTTTCAACAGAAGGCAG+TGG + chr8_4:47844543-47844562 Msa1329330:CDS 40.0%
TAGAAACTGGGATTGGAGAT+CGG + chr8_4:47844629-47844648 Msa1329330:CDS 40.0%
! GGTGAATTTTCGTGAGTTAC+CGG + chr8_4:47844803-47844822 Msa1329330:three_prime_UTR 40.0%
! TCTCTAGAGAAATCCTGAAC+CGG - chr8_4:47844825-47844844 None:intergenic 40.0%
AGAGAGTCAGAGAAAGATGA+TGG + chr8_4:47844840-47844859 Msa1329330:three_prime_UTR 40.0%
! AGAGAAAGATGATGGTTCGT+CGG + chr8_4:47844848-47844867 Msa1329330:three_prime_UTR 40.0%
! AAGGTTAGTTGCAAGCAAGT+GGG - chr8_4:47843645-47843664 None:intergenic 40.0%
! TAAGGTTAGTTGCAAGCAAG+TGG - chr8_4:47843646-47843665 None:intergenic 40.0%
!! GTGTGGTTGGTAGTAAACTA+TGG - chr8_4:47843675-47843694 None:intergenic 40.0%
TGCGAATTGAAGTGATGTTG+GGG - chr8_4:47843736-47843755 None:intergenic 40.0%
! TGCAACCTTTTCGAAGAACT+CGG - chr8_4:47843895-47843914 None:intergenic 40.0%
GTTTCGATCCATTGCAAACA+AGG - chr8_4:47843938-47843957 None:intergenic 40.0%
!! ATATTTACGGACTCTGAGAC+CGG - chr8_4:47844074-47844093 None:intergenic 40.0%
AAGATTCGCGATCCGAATTT+CGG + chr8_4:47844409-47844428 Msa1329330:CDS 40.0%
GAGCTCACGATCTAGTAATA+AGG + chr8_4:47844438-47844457 Msa1329330:CDS 40.0%
AAATCAAGATTAATCGCCGC+GGG - chr8_4:47844511-47844530 None:intergenic 40.0%
!! GATTTTTCAACAGAAGGCAG+TGG + chr8_4:47844543-47844562 Msa1329330:CDS 40.0%
TAGAAACTGGGATTGGAGAT+CGG + chr8_4:47844629-47844648 Msa1329330:CDS 40.0%
! GGTGAATTTTCGTGAGTTAC+CGG + chr8_4:47844803-47844822 Msa1329330:three_prime_UTR 40.0%
! TCTCTAGAGAAATCCTGAAC+CGG - chr8_4:47844825-47844844 None:intergenic 40.0%
AGAGAGTCAGAGAAAGATGA+TGG + chr8_4:47844840-47844859 Msa1329330:three_prime_UTR 40.0%
! AGAGAAAGATGATGGTTCGT+CGG + chr8_4:47844848-47844867 Msa1329330:three_prime_UTR 40.0%
! AGGTTAGTTGCAAGCAAGTG+GGG - chr8_4:47843644-47843663 None:intergenic 45.0%
ATCGAAACACCAGAAGCTCA+AGG + chr8_4:47843950-47843969 Msa1329330:CDS 45.0%
TTGCTACGAAAACAGTAGCG+TGG - chr8_4:47843976-47843995 None:intergenic 45.0%
GCAAAATTCTTTGGTCGAGC+TGG + chr8_4:47843992-47844011 Msa1329330:CDS 45.0%
ATTACCTGGTCTCTGTGGTT+CGG - chr8_4:47844044-47844063 None:intergenic 45.0%
! TCTCTACTGTTTGAAGCGTG+TGG + chr8_4:47844190-47844209 Msa1329330:CDS 45.0%
! GTTTGAAGCGTGTGGAAGAA+CGG + chr8_4:47844198-47844217 Msa1329330:CDS 45.0%
AGAACGGTTAACCCTGTGAA+CGG + chr8_4:47844214-47844233 Msa1329330:CDS 45.0%
! GACTTCTTTGGACTGGAAAC+TGG + chr8_4:47844245-47844264 Msa1329330:CDS 45.0%
AGATTCGCGATCCGAATTTC+GGG + chr8_4:47844410-47844429 Msa1329330:CDS 45.0%
!! AGGTGTGTTCTGGAAGCAAA+CGG + chr8_4:47844458-47844477 Msa1329330:CDS 45.0%
!!! GACGCCGATTTTTCAACAGA+AGG + chr8_4:47844537-47844556 Msa1329330:CDS 45.0%
! TTTTCAACAGAAGGCAGTGG+AGG + chr8_4:47844546-47844565 Msa1329330:CDS 45.0%
GCGTGTTTAGAAACTGGGAT+TGG + chr8_4:47844622-47844641 Msa1329330:CDS 45.0%
!!! TTTTCGTGAGTTACCGGTTC+AGG + chr8_4:47844809-47844828 Msa1329330:three_prime_UTR 45.0%
GATGTGTAAACCTGTAACCG+TGG + chr8_4:47844912-47844931 Msa1329330:three_prime_UTR 45.0%
! AGGTTAGTTGCAAGCAAGTG+GGG - chr8_4:47843644-47843663 None:intergenic 45.0%
ATCGAAACACCAGAAGCTCA+AGG + chr8_4:47843950-47843969 Msa1329330:CDS 45.0%
TTGCTACGAAAACAGTAGCG+TGG - chr8_4:47843976-47843995 None:intergenic 45.0%
GCAAAATTCTTTGGTCGAGC+TGG + chr8_4:47843992-47844011 Msa1329330:CDS 45.0%
ATTACCTGGTCTCTGTGGTT+CGG - chr8_4:47844044-47844063 None:intergenic 45.0%
! TCTCTACTGTTTGAAGCGTG+TGG + chr8_4:47844190-47844209 Msa1329330:CDS 45.0%
! GTTTGAAGCGTGTGGAAGAA+CGG + chr8_4:47844198-47844217 Msa1329330:CDS 45.0%
AGAACGGTTAACCCTGTGAA+CGG + chr8_4:47844214-47844233 Msa1329330:CDS 45.0%
! GACTTCTTTGGACTGGAAAC+TGG + chr8_4:47844245-47844264 Msa1329330:CDS 45.0%
AGATTCGCGATCCGAATTTC+GGG + chr8_4:47844410-47844429 Msa1329330:CDS 45.0%
!! AGGTGTGTTCTGGAAGCAAA+CGG + chr8_4:47844458-47844477 Msa1329330:CDS 45.0%
!!! GACGCCGATTTTTCAACAGA+AGG + chr8_4:47844537-47844556 Msa1329330:CDS 45.0%
! TTTTCAACAGAAGGCAGTGG+AGG + chr8_4:47844546-47844565 Msa1329330:CDS 45.0%
GCGTGTTTAGAAACTGGGAT+TGG + chr8_4:47844622-47844641 Msa1329330:CDS 45.0%
!!! TTTTCGTGAGTTACCGGTTC+AGG + chr8_4:47844809-47844828 Msa1329330:three_prime_UTR 45.0%
GATGTGTAAACCTGTAACCG+TGG + chr8_4:47844912-47844931 Msa1329330:three_prime_UTR 45.0%
! AGAAGAAGAGGAGGTGTGGT+TGG - chr8_4:47843688-47843707 None:intergenic 50.0%
AAGGAGAAGAAGAGGAGGTG+TGG - chr8_4:47843692-47843711 None:intergenic 50.0%
TTCGGGGACGTTGGAAATGA+AGG - chr8_4:47844026-47844045 None:intergenic 50.0%
TTACCTGGTCTCTGTGGTTC+GGG - chr8_4:47844043-47844062 None:intergenic 50.0%
!! ACGGTGCTGTTGGACTTCTT+TGG + chr8_4:47844233-47844252 Msa1329330:CDS 50.0%
GCTGTTGGACTTCTTTGGAC+TGG + chr8_4:47844238-47844257 Msa1329330:CDS 50.0%
TTGTCAAGCAGCGGTTGAGA+CGG + chr8_4:47844273-47844292 Msa1329330:CDS 50.0%
!! AGAAGTGTCAGCTCCGGTAA+AGG - chr8_4:47844322-47844341 None:intergenic 50.0%
TTTACCGGAGCTGACACTTC+TGG + chr8_4:47844321-47844340 Msa1329330:CDS 50.0%
TGCCACTGATGAAGCTTCTG+AGG + chr8_4:47844357-47844376 Msa1329330:CDS 50.0%
! CACTGATGAAGCTTCTGAGG+CGG + chr8_4:47844360-47844379 Msa1329330:CDS 50.0%
!! TGTGTTCTGGAAGCAAACGG+AGG + chr8_4:47844461-47844480 Msa1329330:CDS 50.0%
TGGAAGCAAACGGAGGAGAT+CGG + chr8_4:47844468-47844487 Msa1329330:CDS 50.0%
GAGATCGGAGGAGTTTGTCA+AGG + chr8_4:47844483-47844502 Msa1329330:CDS 50.0%
ACGACGGCGTGTTTAGAAAC+TGG + chr8_4:47844616-47844635 Msa1329330:CDS 50.0%
CGACGGCGTGTTTAGAAACT+GGG + chr8_4:47844617-47844636 Msa1329330:CDS 50.0%
AAACTGGGATTGGAGATCGG+TGG + chr8_4:47844632-47844651 Msa1329330:CDS 50.0%
ATTGGAGATCGGTGGAGTCA+CGG + chr8_4:47844640-47844659 Msa1329330:CDS 50.0%
! AGAAGAAGAGGAGGTGTGGT+TGG - chr8_4:47843688-47843707 None:intergenic 50.0%
AAGGAGAAGAAGAGGAGGTG+TGG - chr8_4:47843692-47843711 None:intergenic 50.0%
TTCGGGGACGTTGGAAATGA+AGG - chr8_4:47844026-47844045 None:intergenic 50.0%
TTACCTGGTCTCTGTGGTTC+GGG - chr8_4:47844043-47844062 None:intergenic 50.0%
!! ACGGTGCTGTTGGACTTCTT+TGG + chr8_4:47844233-47844252 Msa1329330:CDS 50.0%
GCTGTTGGACTTCTTTGGAC+TGG + chr8_4:47844238-47844257 Msa1329330:CDS 50.0%
TTGTCAAGCAGCGGTTGAGA+CGG + chr8_4:47844273-47844292 Msa1329330:CDS 50.0%
!! AGAAGTGTCAGCTCCGGTAA+AGG - chr8_4:47844322-47844341 None:intergenic 50.0%
TTTACCGGAGCTGACACTTC+TGG + chr8_4:47844321-47844340 Msa1329330:CDS 50.0%
TGCCACTGATGAAGCTTCTG+AGG + chr8_4:47844357-47844376 Msa1329330:CDS 50.0%
! CACTGATGAAGCTTCTGAGG+CGG + chr8_4:47844360-47844379 Msa1329330:CDS 50.0%
!! TGTGTTCTGGAAGCAAACGG+AGG + chr8_4:47844461-47844480 Msa1329330:CDS 50.0%
TGGAAGCAAACGGAGGAGAT+CGG + chr8_4:47844468-47844487 Msa1329330:CDS 50.0%
GAGATCGGAGGAGTTTGTCA+AGG + chr8_4:47844483-47844502 Msa1329330:CDS 50.0%
ACGACGGCGTGTTTAGAAAC+TGG + chr8_4:47844616-47844635 Msa1329330:CDS 50.0%
CGACGGCGTGTTTAGAAACT+GGG + chr8_4:47844617-47844636 Msa1329330:CDS 50.0%
AAACTGGGATTGGAGATCGG+TGG + chr8_4:47844632-47844651 Msa1329330:CDS 50.0%
ATTGGAGATCGGTGGAGTCA+CGG + chr8_4:47844640-47844659 Msa1329330:CDS 50.0%
AAGTGGGGAGAGAGACGTGA+AGG - chr8_4:47843629-47843648 None:intergenic 55.0%
GGAGGAAGGAGAAGAAGAGG+AGG - chr8_4:47843697-47843716 None:intergenic 55.0%
AGGGGAGGAAGGAGAAGAAG+AGG - chr8_4:47843700-47843719 None:intergenic 55.0%
!! GGGTGCCGAGTTCTTCGAAA+AGG + chr8_4:47843887-47843906 Msa1329330:CDS 55.0%
TACCTGGTCTCTGTGGTTCG+GGG - chr8_4:47844042-47844061 None:intergenic 55.0%
!! AACCCTGTGAACGGTGCTGT+TGG + chr8_4:47844223-47844242 Msa1329330:CDS 55.0%
GTCCAACAGCACCGTTCACA+GGG - chr8_4:47844228-47844247 None:intergenic 55.0%
AGTCCAACAGCACCGTTCAC+AGG - chr8_4:47844229-47844248 None:intergenic 55.0%
CTGGCACGTTTGTCAAGCAG+CGG + chr8_4:47844264-47844283 Msa1329330:CDS 55.0%
GTTGAGACGGTTCTTCGTGG+TGG + chr8_4:47844286-47844305 Msa1329330:CDS 55.0%
TTCTTCGTGGTGGCACGCTT+AGG + chr8_4:47844296-47844315 Msa1329330:CDS 55.0%
TGGCACGCTTAGGCCTTTAC+CGG + chr8_4:47844306-47844325 Msa1329330:CDS 55.0%
GCTTCATCAGTGGCAGTGAG+AGG - chr8_4:47844352-47844371 None:intergenic 55.0%
CGCCTCAGAAGCTTCATCAG+TGG - chr8_4:47844362-47844381 None:intergenic 55.0%
!! GAAGCTTCTGAGGCGGAAGA+CGG + chr8_4:47844367-47844386 Msa1329330:CDS 55.0%
! TCGCGATCCGAATTTCGGGT+TGG + chr8_4:47844414-47844433 Msa1329330:CDS 55.0%
GAGCTCGCCAACCCGAAATT+CGG - chr8_4:47844424-47844443 None:intergenic 55.0%
AAGCAAACGGAGGAGATCGG+AGG + chr8_4:47844471-47844490 Msa1329330:CDS 55.0%
GGAGTTTGTCAAGGTTCCCG+CGG + chr8_4:47844492-47844511 Msa1329330:CDS 55.0%
AGAAGGCAGTGGAGGAACGA+CGG + chr8_4:47844554-47844573 Msa1329330:CDS 55.0%
TGGTTCACCGTCGATGACGT+CGG + chr8_4:47844579-47844598 Msa1329330:CDS 55.0%
GATTCCTCCGACGTCATCGA+CGG - chr8_4:47844589-47844608 None:intergenic 55.0%
GTCGATGACGTCGGAGGAAT+CGG + chr8_4:47844588-47844607 Msa1329330:CDS 55.0%
AAGTGGGGAGAGAGACGTGA+AGG - chr8_4:47843629-47843648 None:intergenic 55.0%
GGAGGAAGGAGAAGAAGAGG+AGG - chr8_4:47843697-47843716 None:intergenic 55.0%
AGGGGAGGAAGGAGAAGAAG+AGG - chr8_4:47843700-47843719 None:intergenic 55.0%
!! GGGTGCCGAGTTCTTCGAAA+AGG + chr8_4:47843887-47843906 Msa1329330:CDS 55.0%
TACCTGGTCTCTGTGGTTCG+GGG - chr8_4:47844042-47844061 None:intergenic 55.0%
!! AACCCTGTGAACGGTGCTGT+TGG + chr8_4:47844223-47844242 Msa1329330:CDS 55.0%
GTCCAACAGCACCGTTCACA+GGG - chr8_4:47844228-47844247 None:intergenic 55.0%
AGTCCAACAGCACCGTTCAC+AGG - chr8_4:47844229-47844248 None:intergenic 55.0%
CTGGCACGTTTGTCAAGCAG+CGG + chr8_4:47844264-47844283 Msa1329330:CDS 55.0%
GTTGAGACGGTTCTTCGTGG+TGG + chr8_4:47844286-47844305 Msa1329330:CDS 55.0%
TTCTTCGTGGTGGCACGCTT+AGG + chr8_4:47844296-47844315 Msa1329330:CDS 55.0%
TGGCACGCTTAGGCCTTTAC+CGG + chr8_4:47844306-47844325 Msa1329330:CDS 55.0%
GCTTCATCAGTGGCAGTGAG+AGG - chr8_4:47844352-47844371 None:intergenic 55.0%
CGCCTCAGAAGCTTCATCAG+TGG - chr8_4:47844362-47844381 None:intergenic 55.0%
!! GAAGCTTCTGAGGCGGAAGA+CGG + chr8_4:47844367-47844386 Msa1329330:CDS 55.0%
! TCGCGATCCGAATTTCGGGT+TGG + chr8_4:47844414-47844433 Msa1329330:CDS 55.0%
GAGCTCGCCAACCCGAAATT+CGG - chr8_4:47844424-47844443 None:intergenic 55.0%
AAGCAAACGGAGGAGATCGG+AGG + chr8_4:47844471-47844490 Msa1329330:CDS 55.0%
GGAGTTTGTCAAGGTTCCCG+CGG + chr8_4:47844492-47844511 Msa1329330:CDS 55.0%
AGAAGGCAGTGGAGGAACGA+CGG + chr8_4:47844554-47844573 Msa1329330:CDS 55.0%
TGGTTCACCGTCGATGACGT+CGG + chr8_4:47844579-47844598 Msa1329330:CDS 55.0%
GATTCCTCCGACGTCATCGA+CGG - chr8_4:47844589-47844608 None:intergenic 55.0%
GTCGATGACGTCGGAGGAAT+CGG + chr8_4:47844588-47844607 Msa1329330:CDS 55.0%
! GTAGCGTGGCCTTGAGCTTC+TGG - chr8_4:47843962-47843981 None:intergenic 60.0%
! TCTCTGTGGTTCGGGGACGT+TGG - chr8_4:47844035-47844054 None:intergenic 60.0%
GCGGTTGAGACGGTTCTTCG+TGG + chr8_4:47844283-47844302 Msa1329330:CDS 60.0%
!! GCGTCCAGAAGTGTCAGCTC+CGG - chr8_4:47844328-47844347 None:intergenic 60.0%
ACGGTGGCGCTGCTGCTATT+TGG + chr8_4:47844386-47844405 Msa1329330:CDS 60.0%
GACGTCATCGACGGTGAACC+AGG - chr8_4:47844580-47844599 None:intergenic 60.0%
TTCACCGTCGATGACGTCGG+AGG + chr8_4:47844582-47844601 Msa1329330:CDS 60.0%
GGAGGAATCGGTGACGACGA+CGG + chr8_4:47844600-47844619 Msa1329330:CDS 60.0%
GGTGGAGTCACGGTGGTGAT+CGG + chr8_4:47844650-47844669 Msa1329330:CDS 60.0%
! GTAGCGTGGCCTTGAGCTTC+TGG - chr8_4:47843962-47843981 None:intergenic 60.0%
! TCTCTGTGGTTCGGGGACGT+TGG - chr8_4:47844035-47844054 None:intergenic 60.0%
GCGGTTGAGACGGTTCTTCG+TGG + chr8_4:47844283-47844302 Msa1329330:CDS 60.0%
!! GCGTCCAGAAGTGTCAGCTC+CGG - chr8_4:47844328-47844347 None:intergenic 60.0%
ACGGTGGCGCTGCTGCTATT+TGG + chr8_4:47844386-47844405 Msa1329330:CDS 60.0%
GACGTCATCGACGGTGAACC+AGG - chr8_4:47844580-47844599 None:intergenic 60.0%
TTCACCGTCGATGACGTCGG+AGG + chr8_4:47844582-47844601 Msa1329330:CDS 60.0%
GGAGGAATCGGTGACGACGA+CGG + chr8_4:47844600-47844619 Msa1329330:CDS 60.0%
GGTGGAGTCACGGTGGTGAT+CGG + chr8_4:47844650-47844669 Msa1329330:CDS 60.0%
GTCCCCGAACCACAGAGACC+AGG + chr8_4:47844037-47844056 Msa1329330:CDS 65.0%
!! GCTTCTGAGGCGGAAGACGG+TGG + chr8_4:47844370-47844389 Msa1329330:CDS 65.0%
GGAGATCGGTGGAGTCACGG+TGG + chr8_4:47844643-47844662 Msa1329330:CDS 65.0%
GTCCCCGAACCACAGAGACC+AGG + chr8_4:47844037-47844056 Msa1329330:CDS 65.0%
!! GCTTCTGAGGCGGAAGACGG+TGG + chr8_4:47844370-47844389 Msa1329330:CDS 65.0%
GGAGATCGGTGGAGTCACGG+TGG + chr8_4:47844643-47844662 Msa1329330:CDS 65.0%
GCAGTGGAGGAACGACGGCC+TGG + chr8_4:47844559-47844578 Msa1329330:CDS 70.0%
GCAGTGGAGGAACGACGGCC+TGG + chr8_4:47844559-47844578 Msa1329330:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 47843619 47844981 47843619 ID=Msa1329330;Name=Msa1329330
chr8_4 mRNA 47843619 47844981 47843619 ID=Msa1329330-mRNA-1;Parent=Msa1329330;Name=Msa1329330-mRNA-1;_AED=0.04;_eAED=0.04;_QI=256|1|1|1|1|1|2|285|233
chr8_4 exon 47843619 47844058 47843619 ID=Msa1329330-mRNA-1:exon:13680;Parent=Msa1329330-mRNA-1
chr8_4 exon 47844179 47844981 47844179 ID=Msa1329330-mRNA-1:exon:13681;Parent=Msa1329330-mRNA-1
chr8_4 five_prime_UTR 47843619 47843874 47843619 ID=Msa1329330-mRNA-1:five_prime_utr;Parent=Msa1329330-mRNA-1
chr8_4 CDS 47843875 47844058 47843875 ID=Msa1329330-mRNA-1:cds;Parent=Msa1329330-mRNA-1
chr8_4 CDS 47844179 47844696 47844179 ID=Msa1329330-mRNA-1:cds;Parent=Msa1329330-mRNA-1
chr8_4 three_prime_UTR 47844697 47844981 47844697 ID=Msa1329330-mRNA-1:three_prime_utr;Parent=Msa1329330-mRNA-1
Gene Sequence

>Msa1329330

ATGAGTTGCAATGGGTGCCGAGTTCTTCGAAAAGGTTGCAGTGAGTCATGTATTTTACGACCTTGTTTGCAATGGATCGAAACACCAGAAGCTCAAGGCCACGCTACTGTTTTCGTAGCAAAATTCTTTGGTCGAGCTGGTCTCATGTCCTTCATTTCCAACGTCCCCGAACCACAGAGACCAGCTCTGTTTCAGTCTCTACTGTTTGAAGCGTGTGGAAGAACGGTTAACCCTGTGAACGGTGCTGTTGGACTTCTTTGGACTGGAAACTGGCACGTTTGTCAAGCAGCGGTTGAGACGGTTCTTCGTGGTGGCACGCTTAGGCCTTTACCGGAGCTGACACTTCTGGACGCGCCTCTCACTGCCACTGATGAAGCTTCTGAGGCGGAAGACGGTGGCGCTGCTGCTATTTGGAAGATTCGCGATCCGAATTTCGGGTTGGCGAGCTCACGATCTAGTAATAAGGTGTGTTCTGGAAGCAAACGGAGGAGATCGGAGGAGTTTGTCAAGGTTCCCGCGGCGATTAATCTTGATTTACGATTGACGCCGATTTTTCAACAGAAGGCAGTGGAGGAACGACGGCCTGGTTCACCGTCGATGACGTCGGAGGAATCGGTGACGACGACGGCGTGTTTAGAAACTGGGATTGGAGATCGGTGGAGTCACGGTGGTGATCGGAAAGTTCTCAACCTTTTCATATGA
Protein sequence

>Msa1329330

MSCNGCRVLRKGCSESCILRPCLQWIETPEAQGHATVFVAKFFGRAGLMSFISNVPEPQRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHVCQAAVETVLRGGTLRPLPELTLLDAPLTATDEASEAEDGGAAAIWKIRDPNFGLASSRSSNKVCSGSKRRRSEEFVKVPAAINLDLRLTPIFQQKAVEERRPGSPSMTSEESVTTTACLETGIGDRWSHGGDRKVLNLFI*