AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1205180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1205180 MtrunA17_Chr4g0054191 92.899 169 11 1 1 169 1 168 2.82e-115 323
Msa1205180 MtrunA17_Chr4g0059361 36.702 188 99 3 1 169 1 187 4.96e-33 115
Msa1205180 MtrunA17_Chr3g0117971 34.359 195 90 6 1 169 1 183 3.03e-30 108
Msa1205180 MtrunA17_Chr5g0414281 38.764 178 88 5 1 166 1 169 2.61e-29 105
Msa1205180 MtrunA17_Chr8g0364591 37.500 176 98 4 1 168 1 172 2.50e-27 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1205180 AT5G67620.1 54.545 187 61 4 1 169 1 181 4.89e-60 184
Msa1205180 AT5G62900.1 40.000 170 86 4 1 166 1 158 2.02e-30 108
Msa1205180 AT5G50090.1 40.230 174 83 5 1 169 1 158 4.54e-29 105
Msa1205180 AT5G50090.2 39.080 174 79 5 1 169 1 152 8.86e-28 101
Msa1205180 AT5G50090.3 50.000 112 48 3 1 107 1 109 1.03e-24 93.6
Msa1205180 AT1G60010.1 32.609 184 92 4 1 166 1 170 2.67e-24 93.6
Msa1205180 AT1G10530.1 34.444 180 87 5 1 166 1 163 3.98e-23 90.1

Find 75 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAGTACTCTGTTTATTTA+TGG 0.088386 8_1:+61313645 None:intergenic
CCTGATGACACTTTGCTTAT+TGG 0.123509 8_1:-61313166 Msa1205180:CDS
CAGAGTTATTATTAGGATTA+AGG 0.148932 8_1:+61313514 None:intergenic
TTACAAAGCATTGCAGAATT+TGG 0.165154 8_1:-61311123 Msa1205180:CDS
AACAAAGAATTGTTAGAAAA+TGG 0.170409 8_1:-61313383 Msa1205180:exon
CGTAGTACAGAGTTATTATT+AGG 0.216664 8_1:+61313507 None:intergenic
AATTAAGGAAGATTTATTTA+TGG 0.225415 8_1:+61311069 None:intergenic
GGCAATTACATGAATTATAT+TGG 0.238496 8_1:+61311037 None:intergenic
CGAAACTTAATTACCTTAAC+TGG 0.239716 8_1:+61312752 None:intergenic
GCAAGATTGAGAGGATTTAT+TGG 0.272858 8_1:-61313309 Msa1205180:CDS
TATTGGAAGGAACATAATTA+AGG 0.287678 8_1:+61311054 None:intergenic
ATTAAGGAAGATTTATTTAT+GGG 0.290530 8_1:+61311070 None:intergenic
TTCCTTATGTCTGCCTGGTT+TGG 0.310678 8_1:-61310878 Msa1205180:three_prime_UTR
CCAGGCAGACATAAGGAAAA+TGG 0.333487 8_1:+61310883 None:intergenic
AACAAAATAGCATGATATAA+TGG 0.364383 8_1:+61310927 None:intergenic
ACAAAGAATTGTTAGAAAAT+GGG 0.367585 8_1:-61313382 Msa1205180:exon
AAATTCTGCAATGCTTTGTA+AGG 0.387024 8_1:+61311125 None:intergenic
TGACACTTTGCTTATTGGTC+AGG 0.391638 8_1:-61313161 Msa1205180:CDS
ATTGTTGTTGAGTTATGAAA+TGG 0.411921 8_1:+61313457 None:intergenic
GAATTGTCAAGCTGCAGAAT+TGG 0.417620 8_1:-61313359 Msa1205180:CDS
GGGAAATTGTTGAAGGAAAC+TGG 0.431181 8_1:-61312830 Msa1205180:CDS
TCGAAGAAATGTGGTAAACT+TGG 0.433100 8_1:-61312851 Msa1205180:CDS
ACCTTAACTGGACTAGGATT+CGG 0.447919 8_1:+61312764 None:intergenic
GTGGGTTGTAGATGTATTGA+AGG 0.454729 8_1:-61312882 Msa1205180:intron
TGGACTAGGATTCGGAGCTC+TGG 0.457328 8_1:+61312772 None:intergenic
TCCGAATCCTAGTCCAGTTA+AGG 0.460882 8_1:-61312765 Msa1205180:intron
TGTGTTGTGTCAGGTGGAAC+AGG 0.461365 8_1:-61311238 Msa1205180:intron
TCTGCAATGCTTTGTAAGGC+TGG 0.461811 8_1:+61311129 None:intergenic
TTTGTAAGGCTGGCCTCCAT+TGG 0.463118 8_1:+61311139 None:intergenic
TAACAGTTGGGTAATTACTA+AGG 0.472552 8_1:+61310952 None:intergenic
TGCACTACTACAAATTAAGA+AGG 0.472876 8_1:+61311012 None:intergenic
GATATAATGGATTAACAGTT+GGG 0.473192 8_1:+61310940 None:intergenic
TGTAGTAGTGCATTATACAT+AGG 0.474686 8_1:-61311001 Msa1205180:three_prime_UTR
TAAACTTGGGAAATTGTTGA+AGG 0.479398 8_1:-61312837 Msa1205180:CDS
AAGGAAACTGGTGGTAGTCA+TGG 0.487783 8_1:-61312818 Msa1205180:CDS
AGAAGCAATAACAGAAGTGT+TGG 0.498291 8_1:-61311186 Msa1205180:CDS
TGATATAATGGATTAACAGT+TGG 0.498377 8_1:+61310939 None:intergenic
TTCCAAACCAGGCAGACATA+AGG 0.505916 8_1:+61310876 None:intergenic
CCAATAAGCAAAGTGTCATC+AGG 0.507918 8_1:+61313166 None:intergenic
AAGATTTATTTATGGGAGTG+AGG 0.508515 8_1:+61311077 None:intergenic
GCACTACTACAAATTAAGAA+GGG 0.510827 8_1:+61311013 None:intergenic
ATCCTCTCAATCTTGCTCCC+TGG 0.517252 8_1:+61313316 None:intergenic
AGATTTATTTATGGGAGTGA+GGG 0.523438 8_1:+61311078 None:intergenic
CAAAGAATTGTTAGAAAATG+GGG 0.524195 8_1:-61313381 Msa1205180:exon
ATGGATTACTCCCTCCGTCC+CGG 0.524511 8_1:+61313664 None:intergenic
TTGGTCTGTTAGTGCTCATG+AGG 0.527619 8_1:-61313290 Msa1205180:CDS
ACAGTTGTGATTCATCATCC+AGG 0.528907 8_1:-61313334 Msa1205180:CDS
CGAAGAAATGTGGTAAACTT+GGG 0.529302 8_1:-61312850 Msa1205180:CDS
CCATTCTCAGATTTAAGAGT+AGG 0.536503 8_1:+61313220 None:intergenic
TTGTTGGTGGTAGTGGCCAA+TGG 0.537451 8_1:-61311155 Msa1205180:CDS
GAGGTTATGAATTCAAATCC+TGG 0.537803 8_1:-61313271 Msa1205180:CDS
CCTACTCTTAAATCTGAGAA+TGG 0.540696 8_1:-61313220 Msa1205180:CDS
AGAAGCTTGAGATGTTTCAA+TGG 0.541690 8_1:+61313193 None:intergenic
TTGTTGTTGAGTTATGAAAT+GGG 0.543010 8_1:+61313458 None:intergenic
GTCAGTTATTCCGGGACGGA+GGG 0.548543 8_1:-61313674 Msa1205180:five_prime_UTR
TTAATTACCTTAACTGGACT+AGG 0.548847 8_1:+61312758 None:intergenic
AGAATGATGATGAATGATGA+TGG 0.553575 8_1:+61313606 None:intergenic
ATTACATGAATTATATTGGA+AGG 0.554922 8_1:+61311041 None:intergenic
AGGAAACTGGTGGTAGTCAT+GGG 0.570159 8_1:-61312817 Msa1205180:CDS
TTGATGAAGTGAAGTGAAGT+GGG 0.594464 8_1:+61313404 None:intergenic
GTTGATGAAGTGAAGTGAAG+TGG 0.607847 8_1:+61313403 None:intergenic
ATCCAGGGAGCAAGATTGAG+AGG 0.609389 8_1:-61313318 Msa1205180:CDS
CACTACTACAAATTAAGAAG+GGG 0.614071 8_1:+61311014 None:intergenic
GGTCAGTTATTCCGGGACGG+AGG 0.616404 8_1:-61313675 Msa1205180:five_prime_UTR
GAGTTTGCTTCGAAGAAATG+TGG 0.621219 8_1:-61312860 Msa1205180:CDS
CAGTTGTGATTCATCATCCA+GGG 0.623471 8_1:-61313333 Msa1205180:CDS
ACTCAACGAACGGAGAGCAA+TGG 0.634599 8_1:-61311213 Msa1205180:CDS
AAGGCACACATTTCCAAACC+AGG 0.644598 8_1:+61310865 None:intergenic
TTGGTGGTAGTGGCCAATGG+AGG 0.645219 8_1:-61311152 Msa1205180:CDS
ATAGGTCAGTTATTCCGGGA+CGG 0.646482 8_1:-61313678 Msa1205180:five_prime_UTR
GGAACAGGCGACTCAACGAA+CGG 0.652981 8_1:-61311223 Msa1205180:CDS
ACAAGAGCAACATAGTGACC+AGG 0.657647 8_1:+61313253 None:intergenic
ACTACTACAAATTAAGAAGG+GGG 0.677016 8_1:+61311015 None:intergenic
CTACTACAAATTAAGAAGGG+GGG 0.690219 8_1:+61311016 None:intergenic
AAATTGTTGAAGGAAACTGG+TGG 0.844470 8_1:-61312827 Msa1205180:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTATGTTATATTATTATT+TGG + chr8_1:61311505-61311524 None:intergenic 10.0%
!!! ATAAAAAAAAGTGTTAAAAT+AGG + chr8_1:61312960-61312979 None:intergenic 10.0%
!!! TTTATTGATTAAAATTTACA+TGG - chr8_1:61312975-61312994 Msa1205180:intron 10.0%
!!! TTATTGATTAAAATTTACAT+GGG - chr8_1:61312976-61312995 Msa1205180:intron 10.0%
!! AAGAAAAATGTATAAATAAA+GGG + chr8_1:61313177-61313196 None:intergenic 10.0%
!! TAAGAAAAATGTATAAATAA+AGG + chr8_1:61313178-61313197 None:intergenic 10.0%
!!! GACACTTTTTTTTAAAAAAT+AGG - chr8_1:61310917-61310936 Msa1205180:three_prime_UTR 15.0%
!!! TTACTTTTAACAAATTTTAG+AGG - chr8_1:61312051-61312070 Msa1205180:intron 15.0%
!!! AATTCAATTTTTAATTAGGA+CGG - chr8_1:61312101-61312120 Msa1205180:intron 15.0%
!! TGATTGAAAAATAATAATTC+AGG + chr8_1:61312366-61312385 None:intergenic 15.0%
!! AATTAGAGTTATATTGTATT+GGG + chr8_1:61313112-61313131 None:intergenic 15.0%
!! TAATTAGAGTTATATTGTAT+TGG + chr8_1:61313113-61313132 None:intergenic 15.0%
!! AGAAAAATGTATAAATAAAG+GGG + chr8_1:61313176-61313195 None:intergenic 15.0%
!! CAAAAATAAAAGATGAATAA+AGG + chr8_1:61313357-61313376 None:intergenic 15.0%
!! ATTAAGGAAGATTTATTTAT+GGG + chr8_1:61313546-61313565 None:intergenic 15.0%
!! AATTAAGGAAGATTTATTTA+TGG + chr8_1:61313547-61313566 None:intergenic 15.0%
!! AACAAAGAATTGTTAGAAAA+TGG - chr8_1:61311230-61311249 Msa1205180:intron 20.0%
!! ACAAAGAATTGTTAGAAAAT+GGG - chr8_1:61311231-61311250 Msa1205180:intron 20.0%
!! TATTGTTAAATAAGACTAGA+AGG + chr8_1:61311530-61311549 None:intergenic 20.0%
!! AAATTTGCCAAAAAAAATTG+TGG - chr8_1:61311696-61311715 Msa1205180:intron 20.0%
!! AGTAAAACACACAAAATTAA+AGG + chr8_1:61312036-61312055 None:intergenic 20.0%
!!! TTTGTTTAGATTTTACGATT+TGG + chr8_1:61312734-61312753 None:intergenic 20.0%
!!! TGAAATGACTCAATTATTTT+GGG + chr8_1:61313065-61313084 None:intergenic 20.0%
!!! TTGAAATGACTCAATTATTT+TGG + chr8_1:61313066-61313085 None:intergenic 20.0%
!! AAAGTGGAATATATTGAATT+GGG + chr8_1:61313153-61313172 None:intergenic 20.0%
!! TCTAATACAATTGTCATAAA+TGG + chr8_1:61313271-61313290 None:intergenic 20.0%
!! TTAGAAATCATAGTATGATT+TGG - chr8_1:61313286-61313305 Msa1205180:CDS 20.0%
!! ATTACATGAATTATATTGGA+AGG + chr8_1:61313575-61313594 None:intergenic 20.0%
!! AACAAAATAGCATGATATAA+TGG + chr8_1:61313689-61313708 None:intergenic 20.0%
! AAAAAATAGGTCAGTTATTC+CGG - chr8_1:61310930-61310949 Msa1205180:three_prime_UTR 25.0%
! TGTGATAAAAACGTGTATAA+GGG + chr8_1:61311046-61311065 None:intergenic 25.0%
! ATGTGATAAAAACGTGTATA+AGG + chr8_1:61311047-61311066 None:intergenic 25.0%
! CAGAGTTATTATTAGGATTA+AGG + chr8_1:61311102-61311121 None:intergenic 25.0%
!! TTGTTGTTGAGTTATGAAAT+GGG + chr8_1:61311158-61311177 None:intergenic 25.0%
!! ATTGTTGTTGAGTTATGAAA+TGG + chr8_1:61311159-61311178 None:intergenic 25.0%
! CAAAGAATTGTTAGAAAATG+GGG - chr8_1:61311232-61311251 Msa1205180:intron 25.0%
! CTTATTCATAAGAGACATTT+AGG + chr8_1:61311620-61311639 None:intergenic 25.0%
!!! TTCATGTCCACAATTTTTTT+TGG + chr8_1:61311706-61311725 None:intergenic 25.0%
!! AAAATTGTGGACATGAATTT+TGG - chr8_1:61311709-61311728 Msa1205180:intron 25.0%
! GTTTCGTTATAGTTTCAAAT+TGG - chr8_1:61311878-61311897 Msa1205180:intron 25.0%
! TAGATTATCATCGTGAATTT+AGG + chr8_1:61312576-61312595 None:intergenic 25.0%
!!! AAAAAGTGTTAAAATAGGTG+TGG + chr8_1:61312955-61312974 None:intergenic 25.0%
! GAAAGTGGAATATATTGAAT+TGG + chr8_1:61313154-61313173 None:intergenic 25.0%
!!! TTTTATTTTTGTGTTGTGTC+AGG - chr8_1:61313366-61313385 Msa1205180:CDS 25.0%
! TATTGGAAGGAACATAATTA+AGG + chr8_1:61313562-61313581 None:intergenic 25.0%
! GGCAATTACATGAATTATAT+TGG + chr8_1:61313579-61313598 None:intergenic 25.0%
!!! GATATAATGGATTAACAGTT+GGG + chr8_1:61313676-61313695 None:intergenic 25.0%
!!! TGATATAATGGATTAACAGT+TGG + chr8_1:61313677-61313696 None:intergenic 25.0%
AAAAATAGGTCAGTTATTCC+GGG - chr8_1:61310931-61310950 Msa1205180:three_prime_UTR 30.0%
! CAGAGTACTCTGTTTATTTA+TGG + chr8_1:61310971-61310990 None:intergenic 30.0%
! AGAATGATGATGAATGATGA+TGG + chr8_1:61311010-61311029 None:intergenic 30.0%
!!! AAACTGTTTTTTCTCTGACA+AGG + chr8_1:61311079-61311098 None:intergenic 30.0%
CGTAGTACAGAGTTATTATT+AGG + chr8_1:61311109-61311128 None:intergenic 30.0%
! ACATCTAGTTCAACAAAAGT+TGG - chr8_1:61311549-61311568 Msa1205180:intron 30.0%
ATGTCTCTTATGAATAAGAC+CGG - chr8_1:61311622-61311641 Msa1205180:intron 30.0%
TAAACTTGGGAAATTGTTGA+AGG - chr8_1:61311776-61311795 Msa1205180:intron 30.0%
TTAATTACCTTAACTGGACT+AGG + chr8_1:61311858-61311877 None:intergenic 30.0%
CGAAACTTAATTACCTTAAC+TGG + chr8_1:61311864-61311883 None:intergenic 30.0%
AAAGTCAATTGCAAAAAAGG+TGG + chr8_1:61311942-61311961 None:intergenic 30.0%
AGCAAAGTCAATTGCAAAAA+AGG + chr8_1:61311945-61311964 None:intergenic 30.0%
TTATGGATCGAATTCATGAT+GGG - chr8_1:61312234-61312253 Msa1205180:intron 30.0%
TAATTCAGGTGTTAATGCTT+TGG + chr8_1:61312352-61312371 None:intergenic 30.0%
TCTTAGTTAGGTAGTAATAG+TGG + chr8_1:61312456-61312475 None:intergenic 30.0%
ACTATTACTACCTAACTAAG+AGG - chr8_1:61312455-61312474 Msa1205180:intron 30.0%
TTCTTGTAAACCTCTTAGTT+AGG + chr8_1:61312468-61312487 None:intergenic 30.0%
TTTACAAGAAACCGCAAAAT+AGG - chr8_1:61312478-61312497 Msa1205180:intron 30.0%
!!! AAACCGCAAAATAGGTTTTT+TGG - chr8_1:61312486-61312505 Msa1205180:intron 30.0%
! ATGCCAAAAAACCTATTTTG+CGG + chr8_1:61312492-61312511 None:intergenic 30.0%
! GTGAATTTAGGATAACTAAG+TGG + chr8_1:61312564-61312583 None:intergenic 30.0%
TAATCTAGTTGACTATGCAA+TGG - chr8_1:61312589-61312608 Msa1205180:intron 30.0%
TGACTATGCAATGGAATATT+AGG - chr8_1:61312598-61312617 Msa1205180:intron 30.0%
ATCCATCATTGTTTGGATTA+TGG + chr8_1:61312648-61312667 None:intergenic 30.0%
GATAAAAGTTGCAGAACAAA+GGG + chr8_1:61312671-61312690 None:intergenic 30.0%
TGATAAAAGTTGCAGAACAA+AGG + chr8_1:61312672-61312691 None:intergenic 30.0%
AAACAAAAAGTCAGTAGCAT+TGG - chr8_1:61312747-61312766 Msa1205180:intron 30.0%
! ATTGGTGATCTAACAATCAT+AGG - chr8_1:61312765-61312784 Msa1205180:intron 30.0%
TAGAGATTATGTAGCTCAAA+TGG + chr8_1:61312884-61312903 None:intergenic 30.0%
TGTATAAATAAAGGGGAAAG+TGG + chr8_1:61313169-61313188 None:intergenic 30.0%
AAATTCTGCAATGCTTTGTA+AGG + chr8_1:61313491-61313510 None:intergenic 30.0%
!! TTACAAAGCATTGCAGAATT+TGG - chr8_1:61313490-61313509 Msa1205180:five_prime_UTR 30.0%
AGATTTATTTATGGGAGTGA+GGG + chr8_1:61313538-61313557 None:intergenic 30.0%
AAGATTTATTTATGGGAGTG+AGG + chr8_1:61313539-61313558 None:intergenic 30.0%
ACTACTACAAATTAAGAAGG+GGG + chr8_1:61313601-61313620 None:intergenic 30.0%
CACTACTACAAATTAAGAAG+GGG + chr8_1:61313602-61313621 None:intergenic 30.0%
GCACTACTACAAATTAAGAA+GGG + chr8_1:61313603-61313622 None:intergenic 30.0%
TGCACTACTACAAATTAAGA+AGG + chr8_1:61313604-61313623 None:intergenic 30.0%
TGTAGTAGTGCATTATACAT+AGG - chr8_1:61313612-61313631 Msa1205180:five_prime_UTR 30.0%
TAACAGTTGGGTAATTACTA+AGG + chr8_1:61313664-61313683 None:intergenic 30.0%
TTGATGAAGTGAAGTGAAGT+GGG + chr8_1:61311212-61311231 None:intergenic 35.0%
GCAAGATTGAGAGGATTTAT+TGG - chr8_1:61311304-61311323 Msa1205180:intron 35.0%
GAGGTTATGAATTCAAATCC+TGG - chr8_1:61311342-61311361 Msa1205180:intron 35.0%
CCATTCTCAGATTTAAGAGT+AGG + chr8_1:61311396-61311415 None:intergenic 35.0%
CCTACTCTTAAATCTGAGAA+TGG - chr8_1:61311393-61311412 Msa1205180:intron 35.0%
AGAAGCTTGAGATGTTTCAA+TGG + chr8_1:61311423-61311442 None:intergenic 35.0%
!! TATCGCTTGATCAGTTTTGA+AGG - chr8_1:61311477-61311496 Msa1205180:intron 35.0%
! ATTGTGGACATGAATTTTGG+TGG - chr8_1:61311712-61311731 Msa1205180:intron 35.0%
!! TTGTGGACATGAATTTTGGT+GGG - chr8_1:61311713-61311732 Msa1205180:intron 35.0%
TCGAAGAAATGTGGTAAACT+TGG - chr8_1:61311762-61311781 Msa1205180:intron 35.0%
CGAAGAAATGTGGTAAACTT+GGG - chr8_1:61311763-61311782 Msa1205180:intron 35.0%
AAATTGTTGAAGGAAACTGG+TGG - chr8_1:61311786-61311805 Msa1205180:intron 35.0%
CTCTTGTTAGACTTAGAGTA+AGG - chr8_1:61311908-61311927 Msa1205180:intron 35.0%
AAACCGATCGTTATACCTTA+TGG - chr8_1:61312217-61312236 Msa1205180:intron 35.0%
GATCCATAAGGTATAACGAT+CGG + chr8_1:61312223-61312242 None:intergenic 35.0%
CATCATGAATTCGATCCATA+AGG + chr8_1:61312235-61312254 None:intergenic 35.0%
CTTATGGATCGAATTCATGA+TGG - chr8_1:61312233-61312252 Msa1205180:intron 35.0%
! TCATAGCAACCAAAAGGTTT+TGG + chr8_1:61312423-61312442 None:intergenic 35.0%
!!! AAATAGGTTTTTTGGCATGC+AGG - chr8_1:61312494-61312513 Msa1205180:intron 35.0%
!!! AATAGGTTTTTTGGCATGCA+GGG - chr8_1:61312495-61312514 Msa1205180:intron 35.0%
CTCCATAATCCAAACAATGA+TGG - chr8_1:61312643-61312662 Msa1205180:intron 35.0%
GTGATCTAACAATCATAGGA+CGG - chr8_1:61312769-61312788 Msa1205180:CDS 35.0%
! AGGGAATAGTAAATTTGGTG+CGG + chr8_1:61313002-61313021 None:intergenic 35.0%
GTAAATGAGTCAGATATTCC+AGG + chr8_1:61313029-61313048 None:intergenic 35.0%
AGGGAGTAAAGTGTATTGTT+TGG - chr8_1:61313240-61313259 Msa1205180:CDS 35.0%
! GGCATTTTCTCTTTACATCT+TGG - chr8_1:61313307-61313326 Msa1205180:CDS 35.0%
!! ATAAAGGCACTGCATATGAT+AGG + chr8_1:61313341-61313360 None:intergenic 35.0%
!! TATTTTTGTGTTGTGTCAGG+TGG - chr8_1:61313369-61313388 Msa1205180:exon 35.0%
! AGAAGCAATAACAGAAGTGT+TGG - chr8_1:61313427-61313446 Msa1205180:five_prime_UTR 35.0%
!! AGTGTTGGAAGACATTTTGT+TGG - chr8_1:61313442-61313461 Msa1205180:five_prime_UTR 35.0%
CTACTACAAATTAAGAAGGG+GGG + chr8_1:61313600-61313619 None:intergenic 35.0%
! GTTGATGAAGTGAAGTGAAG+TGG + chr8_1:61311213-61311232 None:intergenic 40.0%
GAATTGTCAAGCTGCAGAAT+TGG - chr8_1:61311254-61311273 Msa1205180:intron 40.0%
ACAGTTGTGATTCATCATCC+AGG - chr8_1:61311279-61311298 Msa1205180:intron 40.0%
CAGTTGTGATTCATCATCCA+GGG - chr8_1:61311280-61311299 Msa1205180:intron 40.0%
! CCAATAAGCAAAGTGTCATC+AGG + chr8_1:61311450-61311469 None:intergenic 40.0%
CCTGATGACACTTTGCTTAT+TGG - chr8_1:61311447-61311466 Msa1205180:intron 40.0%
TGACACTTTGCTTATTGGTC+AGG - chr8_1:61311452-61311471 Msa1205180:intron 40.0%
GGTATGCCTTAGTCGAAATT+TGG - chr8_1:61311570-61311589 Msa1205180:intron 40.0%
TGCTGTCCAAATTTCGACTA+AGG + chr8_1:61311579-61311598 None:intergenic 40.0%
TCTCTTATGAATAAGACCGG+AGG - chr8_1:61311625-61311644 Msa1205180:intron 40.0%
CTCTTATGAATAAGACCGGA+GGG - chr8_1:61311626-61311645 Msa1205180:intron 40.0%
TTGAAAATTGACACTCCCTC+CGG + chr8_1:61311644-61311663 None:intergenic 40.0%
! GTGGGTTGTAGATGTATTGA+AGG - chr8_1:61311731-61311750 Msa1205180:intron 40.0%
! GAGTTTGCTTCGAAGAAATG+TGG - chr8_1:61311753-61311772 Msa1205180:intron 40.0%
GGGAAATTGTTGAAGGAAAC+TGG - chr8_1:61311783-61311802 Msa1205180:intron 40.0%
! ACCTTAACTGGACTAGGATT+CGG + chr8_1:61311852-61311871 None:intergenic 40.0%
! TATAACGATCGGTTTCAACG+TGG + chr8_1:61312212-61312231 None:intergenic 40.0%
! GGTGTTAATGCTTTGGATCT+CGG + chr8_1:61312345-61312364 None:intergenic 40.0%
!! AGCAACCAAAAGGTTTTGGT+AGG + chr8_1:61312419-61312438 None:intergenic 40.0%
CCAACATCATAGCAACCAAA+AGG + chr8_1:61312429-61312448 None:intergenic 40.0%
!! CCTTTTGGTTGCTATGATGT+TGG - chr8_1:61312426-61312445 Msa1205180:intron 40.0%
!!! ATAGGTTTTTTGGCATGCAG+GGG - chr8_1:61312496-61312515 Msa1205180:intron 40.0%
CAAAGGGATCCATCATTGTT+TGG + chr8_1:61312655-61312674 None:intergenic 40.0%
TTCATGAGATGGAAACGTGA+AGG + chr8_1:61312704-61312723 None:intergenic 40.0%
!! TTGGTAGTGCTTTCATGAGA+TGG + chr8_1:61312715-61312734 None:intergenic 40.0%
CTAACAATCATAGGACGGTA+TGG - chr8_1:61312774-61312793 Msa1205180:CDS 40.0%
GACGGAGGGAATAGTAAATT+TGG + chr8_1:61313007-61313026 None:intergenic 40.0%
ATGAGTCAGATATTCCAGGA+CGG + chr8_1:61313025-61313044 None:intergenic 40.0%
! CCAAACTGACTCAGATATTC+CGG - chr8_1:61313212-61313231 Msa1205180:CDS 40.0%
! CAAACTGACTCAGATATTCC+GGG - chr8_1:61313213-61313232 Msa1205180:CDS 40.0%
! GTTGGAAGACATTTTGTTGG+TGG - chr8_1:61313445-61313464 Msa1205180:five_prime_UTR 40.0%
!!! AGACATTTTGTTGGTGGTAG+TGG - chr8_1:61313451-61313470 Msa1205180:five_prime_UTR 40.0%
ATAGGTCAGTTATTCCGGGA+CGG - chr8_1:61310935-61310954 Msa1205180:three_prime_UTR 45.0%
TTGGTCTGTTAGTGCTCATG+AGG - chr8_1:61311323-61311342 Msa1205180:intron 45.0%
ACAAGAGCAACATAGTGACC+AGG + chr8_1:61311363-61311382 None:intergenic 45.0%
AAGGAAACTGGTGGTAGTCA+TGG - chr8_1:61311795-61311814 Msa1205180:intron 45.0%
AGGAAACTGGTGGTAGTCAT+GGG - chr8_1:61311796-61311815 Msa1205180:intron 45.0%
TCCGAATCCTAGTCCAGTTA+AGG - chr8_1:61311848-61311867 Msa1205180:intron 45.0%
! TGCGTCCTACCAAAACCTTT+TGG - chr8_1:61312411-61312430 Msa1205180:intron 45.0%
ATTTACTATTCCCTCCGTCC+TGG - chr8_1:61313008-61313027 Msa1205180:intron 45.0%
CCGGAATATCTGAGTCAGTT+TGG + chr8_1:61313215-61313234 None:intergenic 45.0%
TCTGCAATGCTTTGTAAGGC+TGG + chr8_1:61313487-61313506 None:intergenic 45.0%
CCAGGCAGACATAAGGAAAA+TGG + chr8_1:61313733-61313752 None:intergenic 45.0%
! CCATTTTCCTTATGTCTGCC+TGG - chr8_1:61313730-61313749 Msa1205180:five_prime_UTR 45.0%
TTCCTTATGTCTGCCTGGTT+TGG - chr8_1:61313735-61313754 Msa1205180:five_prime_UTR 45.0%
TTCCAAACCAGGCAGACATA+AGG + chr8_1:61313740-61313759 None:intergenic 45.0%
!!! TTGAATTTTAAATTTATAAT+AGG + chr8_1:61312088-61312107 None:intergenic 5.0%
!!! TTAAAATTCAATTTTTAATT+AGG - chr8_1:61312097-61312116 Msa1205180:intron 5.0%
!! TTATAAAAAAAAAAAAGTAA+AGG - chr8_1:61312828-61312847 Msa1205180:CDS 5.0%
ATCCAGGGAGCAAGATTGAG+AGG - chr8_1:61311295-61311314 Msa1205180:intron 50.0%
ATCCTCTCAATCTTGCTCCC+TGG + chr8_1:61311300-61311319 None:intergenic 50.0%
GTCAGATATTCCAGGACGGA+GGG + chr8_1:61313021-61313040 None:intergenic 50.0%
AGTCAGATATTCCAGGACGG+AGG + chr8_1:61313022-61313041 None:intergenic 50.0%
CTGACTCAGATATTCCGGGA+CGG - chr8_1:61313217-61313236 Msa1205180:CDS 50.0%
TGTGTTGTGTCAGGTGGAAC+AGG - chr8_1:61313375-61313394 Msa1205180:exon 50.0%
ACTCAACGAACGGAGAGCAA+TGG - chr8_1:61313400-61313419 Msa1205180:five_prime_UTR 50.0%
!! TTGTTGGTGGTAGTGGCCAA+TGG - chr8_1:61313458-61313477 Msa1205180:five_prime_UTR 50.0%
!! TTTGTAAGGCTGGCCTCCAT+TGG + chr8_1:61313477-61313496 None:intergenic 50.0%
GTCAGTTATTCCGGGACGGA+GGG - chr8_1:61310939-61310958 Msa1205180:three_prime_UTR 55.0%
! ATGGATTACTCCCTCCGTCC+CGG + chr8_1:61310952-61310971 None:intergenic 55.0%
! TGGACTAGGATTCGGAGCTC+TGG + chr8_1:61311844-61311863 None:intergenic 55.0%
! TTTGGCATGCAGGGGCACAT+AGG - chr8_1:61312504-61312523 Msa1205180:intron 55.0%
ACTCAGATATTCCGGGACGG+AGG - chr8_1:61313220-61313239 Msa1205180:CDS 55.0%
CTCAGATATTCCGGGACGGA+GGG - chr8_1:61313221-61313240 Msa1205180:CDS 55.0%
ACACTTTACTCCCTCCGTCC+CGG + chr8_1:61313234-61313253 None:intergenic 55.0%
GGAACAGGCGACTCAACGAA+CGG - chr8_1:61313390-61313409 Msa1205180:five_prime_UTR 55.0%
!! TTGGTGGTAGTGGCCAATGG+AGG - chr8_1:61313461-61313480 Msa1205180:five_prime_UTR 55.0%
GGTCAGTTATTCCGGGACGG+AGG - chr8_1:61310938-61310957 Msa1205180:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 61310868 61313767 61310868 ID=Msa1205180;Name=Msa1205180
chr8_1 mRNA 61310868 61313767 61310868 ID=Msa1205180-mRNA-1;Parent=Msa1205180;Name=Msa1205180-mRNA-1;_AED=0.02;_eAED=0.02;_QI=381|1|1|1|1|1|3|248|169
chr8_1 exon 61310868 61311247 61310868 ID=Msa1205180-mRNA-1:exon:19905;Parent=Msa1205180-mRNA-1
chr8_1 exon 61312766 61312893 61312766 ID=Msa1205180-mRNA-1:exon:19904;Parent=Msa1205180-mRNA-1
chr8_1 exon 61313137 61313767 61313137 ID=Msa1205180-mRNA-1:exon:19903;Parent=Msa1205180-mRNA-1
chr8_1 five_prime_UTR 61313387 61313767 61313387 ID=Msa1205180-mRNA-1:five_prime_utr;Parent=Msa1205180-mRNA-1
chr8_1 CDS 61313137 61313386 61313137 ID=Msa1205180-mRNA-1:cds;Parent=Msa1205180-mRNA-1
chr8_1 CDS 61312766 61312893 61312766 ID=Msa1205180-mRNA-1:cds;Parent=Msa1205180-mRNA-1
chr8_1 CDS 61311116 61311247 61311116 ID=Msa1205180-mRNA-1:cds;Parent=Msa1205180-mRNA-1
chr8_1 three_prime_UTR 61310868 61311115 61310868 ID=Msa1205180-mRNA-1:three_prime_utr;Parent=Msa1205180-mRNA-1
Gene Sequence

>Msa1205180

ATGGGGAATTGTCAAGCTGCAGAATTGGCTACAGTTGTGATTCATCATCCAGGGAGCAAGATTGAGAGGATTTATTGGTCTGTTAGTGCTCATGAGGTTATGAATTCAAATCCTGGTCACTATGTTGCTCTTGTTGTTTCATCACCTACTCTTAAATCTGAGAATGGTATGCCATTGAAACATCTCAAGCTTCTTCGACCTGATGACACTTTGCTTATTGGTCAGGTTTATCGCTTGATCAGTTTTGAAGATGTATTGAAGGAGTTTGCTTCGAAGAAATGTGGTAAACTTGGGAAATTGTTGAAGGAAACTGGTGGTAGTCATGGGATTCAGATGAAGCATAGAGACTCCAGAGCTCCGAATCCTAGTCCAGTTAAGGTGGAACAGGCGACTCAACGAACGGAGAGCAATGGCAGCAGAAGCAATAACAGAAGTGTTGGAAGACATTTTGTTGGTGGTAGTGGCCAATGGAGGCCAGCCTTACAAAGCATTGCAGAATTTGGAACTTGA
Protein sequence

>Msa1205180

MGNCQAAELATVVIHHPGSKIERIYWSVSAHEVMNSNPGHYVALVVSSPTLKSENGMPLKHLKLLRPDDTLLIGQVYRLISFEDVLKEFASKKCGKLGKLLKETGGSHGIQMKHRDSRAPNPSPVKVEQATQRTESNGSRSNNRSVGRHFVGGSGQWRPALQSIAEFGT*