AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1214510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1214510 MtrunA17_Chr4g0062691 97.179 390 7 4 1 388 1 388 0.0 766
Msa1214510 MtrunA17_Chr6g0456881 41.176 170 85 3 110 266 105 272 1.48e-28 115
Msa1214510 MtrunA17_Chr7g0220931 66.129 62 21 0 133 194 122 183 3.52e-21 94.0
Msa1214510 MtrunA17_Chr1g0205131 55.696 79 33 1 119 195 95 173 1.30e-19 89.7
Msa1214510 MtrunA17_Chr8g0376571 35.329 167 83 6 127 268 57 223 2.75e-17 83.6
Msa1214510 MtrunA17_Chr8g0360441 45.918 98 51 2 109 205 31 127 4.52e-17 82.4
Msa1214510 MtrunA17_Chr7g0219461 32.035 231 129 9 132 358 58 264 8.56e-17 80.9
Msa1214510 MtrunA17_Chr2g0316391 48.718 78 39 1 113 190 3 79 3.34e-15 75.5
Msa1214510 MtrunA17_Chr6g0454781 58.621 58 24 0 132 189 50 107 4.90e-15 75.5
Msa1214510 MtrunA17_Chr4g0040921 50.000 64 32 0 127 190 18 81 7.64e-15 75.1
Msa1214510 MtrunA17_Chr3g0086961 33.099 142 87 2 132 268 58 196 1.97e-14 74.3
Msa1214510 MtrunA17_Chr2g0325171 49.206 63 32 0 127 189 20 82 2.12e-14 73.6
Msa1214510 MtrunA17_Chr4g0061561 48.276 58 30 0 132 189 58 115 4.63e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1214510 AT3G18550.1 32.131 305 157 11 1 263 5 301 3.83e-25 106
Msa1214510 AT3G18550.4 32.131 305 157 11 1 263 5 301 3.83e-25 106
Msa1214510 AT3G18550.3 32.131 305 157 11 1 263 5 301 4.51e-25 106
Msa1214510 AT3G18550.2 36.538 208 109 5 1 190 5 207 8.00e-25 105
Msa1214510 AT1G67260.2 51.546 97 39 1 108 196 56 152 1.23e-23 101
Msa1214510 AT1G67260.3 51.546 97 39 1 108 196 56 152 1.23e-23 101
Msa1214510 AT1G67260.1 51.546 97 39 1 108 196 56 152 1.38e-23 101
Msa1214510 AT1G68800.2 65.217 69 23 1 122 189 102 170 2.24e-22 97.8
Msa1214510 AT1G68800.1 65.217 69 23 1 122 189 102 170 2.34e-22 97.4
Msa1214510 AT4G18390.1 35.519 183 78 6 127 293 38 196 1.15e-19 90.1
Msa1214510 AT4G18390.2 35.519 183 78 6 127 293 38 196 1.15e-19 90.1
Msa1214510 AT3G15030.2 44.898 98 53 1 94 190 7 104 4.20e-17 82.8
Msa1214510 AT3G15030.4 44.898 98 53 1 94 190 7 104 4.20e-17 82.8
Msa1214510 AT3G15030.3 44.898 98 53 1 94 190 7 104 4.20e-17 82.8
Msa1214510 AT3G15030.1 44.898 98 53 1 94 190 7 104 4.20e-17 82.8
Msa1214510 AT1G53230.1 49.383 81 40 1 112 191 29 109 6.29e-16 79.3
Msa1214510 AT1G53230.2 49.383 81 40 1 112 191 29 109 6.29e-16 79.3
Msa1214510 AT1G30210.2 36.879 141 65 3 127 267 46 162 8.59e-15 75.1
Msa1214510 AT1G30210.1 36.879 141 65 3 127 267 46 162 8.59e-15 75.1
Msa1214510 AT2G31070.1 45.000 80 43 1 111 189 8 87 4.99e-14 73.2
Msa1214510 AT3G02150.1 53.448 58 27 0 132 189 75 132 2.55e-13 70.1
Msa1214510 AT3G02150.2 53.448 58 27 0 132 189 75 132 4.48e-13 70.5
Msa1214510 AT5G60970.1 48.333 60 31 0 130 189 62 121 1.01e-12 69.3
Msa1214510 AT5G08070.2 48.276 58 30 0 132 189 34 91 1.79e-11 64.3
Msa1214510 AT5G08070.1 48.276 58 30 0 132 189 34 91 1.79e-11 64.3

Find 83 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATATCATCAGGATAATT+AGG 0.171473 8_1:+75179069 None:intergenic
TTATTTCTTTCCTTTCCTTT+TGG 0.203378 8_1:-75177870 Msa1214510:three_prime_UTR
GAGAAGAAATAGTACTAATA+AGG 0.277919 8_1:-75178524 Msa1214510:CDS
CTGTTTGTTGAATACATATC+TGG 0.295320 8_1:+75179228 None:intergenic
GAGAAAGGAGGAAGAGGAAA+GGG 0.310050 8_1:+75179105 None:intergenic
TCTGTTCCTGACCTTGAAAT+TGG 0.315222 8_1:-75177740 Msa1214510:three_prime_UTR
TTGCTTTCAAAGGAACAAAA+AGG 0.336731 8_1:+75179165 None:intergenic
GTGAGGTATCTTCAGAATTT+CGG 0.343155 8_1:-75177836 Msa1214510:three_prime_UTR
CATGTTGTTGGAGGAAGATT+TGG 0.344830 8_1:+75179028 None:intergenic
TTTGGTCTTCAAGATATGTT+AGG 0.353164 8_1:-75178745 Msa1214510:CDS
TGAGCCTGACTGACACAAAA+AGG 0.360607 8_1:+75177920 None:intergenic
AGAAAGGAGGAAGAGGAAAG+GGG 0.382698 8_1:+75179106 None:intergenic
GATATATTTATAATACTGTT+TGG 0.387916 8_1:-75179290 Msa1214510:five_prime_UTR
AAGGTGTTTGATCTCAAGTT+TGG 0.394081 8_1:+75178678 None:intergenic
AAAGGAGGTGGTGATGATAA+GGG 0.399159 8_1:+75177938 None:intergenic
TGCTAGGTTATTTCCATTGA+GGG 0.401156 8_1:+75179203 None:intergenic
GTAAACTTGTACCAATTTCA+AGG 0.404054 8_1:+75177729 None:intergenic
GAACAGAAACAAACAAGAAA+GGG 0.406969 8_1:+75177756 None:intergenic
CAATATCATCAGGATAATTA+GGG 0.427407 8_1:+75179070 None:intergenic
AAAAGGAGGTGGTGATGATA+AGG 0.428540 8_1:+75177937 None:intergenic
AGAGAAAGGAGGAAGAGGAA+AGG 0.429249 8_1:+75179104 None:intergenic
AATTTGAACTTACTTGTTGA+AGG 0.433582 8_1:+75178131 None:intergenic
GCTTTGATTGTTGAGTTTGA+AGG 0.434606 8_1:+75178934 None:intergenic
CTTCCTGCAGCTGCTGGTAA+AGG 0.436015 8_1:-75178898 Msa1214510:CDS
TCAAGCTTGGATAATGAAGT+TGG 0.437838 8_1:-75178586 Msa1214510:CDS
TTGTTATTCATCATCACAAC+AGG 0.440557 8_1:+75178979 None:intergenic
CTTTGTTCATTATGAGCAAA+TGG 0.450640 8_1:-75178186 Msa1214510:CDS
TTGAGAGAATTGAACATCAT+AGG 0.458304 8_1:+75178157 None:intergenic
CATCAAGACATGTACTAGCT+AGG 0.462310 8_1:-75179346 Msa1214510:five_prime_UTR
GGAAATCAACATCATGTTGT+TGG 0.463207 8_1:+75179016 None:intergenic
TCAAAACCGCGAAAGGACTA+AGG 0.463298 8_1:-75178810 Msa1214510:CDS
CTTGTACCAATTTCAAGGTC+AGG 0.464630 8_1:+75177734 None:intergenic
CAAGAGAAAGGGCAAGAGAA+AGG 0.468363 8_1:-75178459 Msa1214510:CDS
AGATTCTTCCACACTTTCAA+AGG 0.468610 8_1:+75178339 None:intergenic
TTTCAAAGGGGTTCCAAATG+AGG 0.468951 8_1:+75178353 None:intergenic
GATTTGGTGGCTGTCTTGAA+AGG 0.472274 8_1:+75179044 None:intergenic
GAAAGGAGGAAGAGGAAAGG+GGG 0.475237 8_1:+75179107 None:intergenic
GGGAATTGAAAGAAAGAGAA+AGG 0.476940 8_1:+75179090 None:intergenic
AAGAGTGCATTCAATTCTAT+AGG 0.477613 8_1:-75178493 Msa1214510:CDS
CCGATCCCTTAGTCCTTTCG+CGG 0.487376 8_1:+75178804 None:intergenic
AATCTTCTTCCTGCAGCTGC+TGG 0.488028 8_1:-75178904 Msa1214510:CDS
GATTCTTCCACACTTTCAAA+GGG 0.488241 8_1:+75178340 None:intergenic
GGAACAGAAACAAACAAGAA+AGG 0.496658 8_1:+75177755 None:intergenic
TCAACAAACAGTTCCCTCAA+TGG 0.498770 8_1:-75179216 Msa1214510:CDS
AAGAAAGAGAAAGGAGGAAG+AGG 0.499947 8_1:+75179099 None:intergenic
CAACAGGTGGATGATGAAGT+TGG 0.500835 8_1:+75178995 None:intergenic
GTTTGATCTCAAGTTTGGAT+TGG 0.508056 8_1:+75178683 None:intergenic
TGAAAGGGATCAATATCATC+AGG 0.511469 8_1:+75179060 None:intergenic
GTTGTTGGAGGAAGATTTGG+TGG 0.511656 8_1:+75179031 None:intergenic
TGAACAATTTCACCAATCCA+TGG 0.514462 8_1:-75178019 Msa1214510:CDS
TCAAAGCAATACAATGAAGA+AGG 0.515193 8_1:-75178388 Msa1214510:CDS
GATGATAAGGGGTGCAAGTA+CGG 0.515892 8_1:+75177950 None:intergenic
ATGCTAGGTTATTTCCATTG+AGG 0.517513 8_1:+75179202 None:intergenic
TCACCACATAACATTCCAAA+AGG 0.520050 8_1:+75177855 None:intergenic
TACTGAAGGAGTTTCAAGCT+TGG 0.530351 8_1:-75178599 Msa1214510:CDS
TGAAGTTGAGTTGCTTTCAA+AGG 0.532484 8_1:+75179155 None:intergenic
TTGCCTTTACCAGCAGCTGC+AGG 0.547785 8_1:+75178895 None:intergenic
ATCAAGACATGTACTAGCTA+GGG 0.547923 8_1:-75179345 Msa1214510:five_prime_UTR
GAAAGTGTGGAAGAATCTGC+AGG 0.550929 8_1:-75178334 Msa1214510:CDS
TTGGAACCCCTTTGAAAGTG+TGG 0.551418 8_1:-75178347 Msa1214510:CDS
ATTTGGTGGCTGTCTTGAAA+GGG 0.555885 8_1:+75179045 None:intergenic
GTGATTTAGAGGAAAATGCT+AGG 0.566051 8_1:+75179187 None:intergenic
AGCAAGATCAAAACCGCGAA+AGG 0.567602 8_1:-75178817 Msa1214510:CDS
AATCAACATCATGTTGTTGG+AGG 0.575800 8_1:+75179019 None:intergenic
CAAAACCGCGAAAGGACTAA+GGG 0.576240 8_1:-75178809 Msa1214510:CDS
TCAACTTCTGAATGTACTGA+AGG 0.578330 8_1:-75178613 Msa1214510:CDS
AATCAGCTTCACATGATCAA+AGG 0.583940 8_1:+75178276 None:intergenic
AACAGAAACAAACAAGAAAG+GGG 0.594346 8_1:+75177757 None:intergenic
AAGCAAGCAAAACAGTGGAC+TGG 0.594986 8_1:-75178714 Msa1214510:CDS
CCGCGAAAGGACTAAGGGAT+CGG 0.598651 8_1:-75178804 Msa1214510:CDS
TTATTCATCATCACAACAGG+TGG 0.603901 8_1:+75178982 None:intergenic
TGTCACTTGAAGTTGCAAAG+AGG 0.614161 8_1:-75178771 Msa1214510:CDS
ACATAACATTCCAAAAGGAA+AGG 0.614793 8_1:+75177860 None:intergenic
AAGGGCAAGAGAAAGGACAA+AGG 0.615637 8_1:-75178452 Msa1214510:CDS
AATTGAAAGAAAGAGAAAGG+AGG 0.616668 8_1:+75179093 None:intergenic
AGCTTCACATGATCAAAGGA+AGG 0.617475 8_1:+75178280 None:intergenic
TGGTAAAGGCAAAACTGTAG+TGG 0.630471 8_1:-75178884 Msa1214510:CDS
AAGGAGGTGGTGATGATAAG+GGG 0.632930 8_1:+75177939 None:intergenic
GGTAGCATGTTAATTAGATG+CGG 0.636554 8_1:+75177971 None:intergenic
AGAGAATTGAACATCATAGG+TGG 0.646080 8_1:+75178160 None:intergenic
ATTCTTCCACACTTTCAAAG+GGG 0.682878 8_1:+75178341 None:intergenic
TCATGTGAAGCTGATTGATG+AGG 0.717288 8_1:-75178269 Msa1214510:CDS
ACAGTACTGATCATCATCAG+CGG 0.729484 8_1:-75178957 Msa1214510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATATTTATAATACTGTT+TGG - chr8_1:75177780-75177799 Msa1214510:three_prime_UTR 15.0%
!! GATATATTTATAATACTGTT+TGG - chr8_1:75177780-75177799 Msa1214510:three_prime_UTR 15.0%
! CAATATCATCAGGATAATTA+GGG + chr8_1:75178003-75178022 None:intergenic 25.0%
! TCAATATCATCAGGATAATT+AGG + chr8_1:75178004-75178023 None:intergenic 25.0%
!!! ATAACAAGTTTTTGTTGTTG+TGG + chr8_1:75178523-75178542 None:intergenic 25.0%
! GAGAAGAAATAGTACTAATA+AGG - chr8_1:75178546-75178565 Msa1214510:CDS 25.0%
! GAATAAAAACTAACCTCATT+TGG - chr8_1:75178704-75178723 Msa1214510:CDS 25.0%
!! AATTTGAACTTACTTGTTGA+AGG + chr8_1:75178942-75178961 None:intergenic 25.0%
!!! TTATTTCTTTCCTTTCCTTT+TGG - chr8_1:75179200-75179219 Msa1214510:CDS 25.0%
! CAATATCATCAGGATAATTA+GGG + chr8_1:75178003-75178022 None:intergenic 25.0%
! TCAATATCATCAGGATAATT+AGG + chr8_1:75178004-75178023 None:intergenic 25.0%
!!! ATAACAAGTTTTTGTTGTTG+TGG + chr8_1:75178523-75178542 None:intergenic 25.0%
! GAGAAGAAATAGTACTAATA+AGG - chr8_1:75178546-75178565 Msa1214510:CDS 25.0%
! GAATAAAAACTAACCTCATT+TGG - chr8_1:75178704-75178723 Msa1214510:CDS 25.0%
!! AATTTGAACTTACTTGTTGA+AGG + chr8_1:75178942-75178961 None:intergenic 25.0%
!!! TTATTTCTTTCCTTTCCTTT+TGG - chr8_1:75179200-75179219 Msa1214510:CDS 25.0%
! CTGTTTGTTGAATACATATC+TGG + chr8_1:75177845-75177864 None:intergenic 30.0%
TTGCTTTCAAAGGAACAAAA+AGG + chr8_1:75177908-75177927 None:intergenic 30.0%
AATTGAAAGAAAGAGAAAGG+AGG + chr8_1:75177980-75177999 None:intergenic 30.0%
! TTGTTATTCATCATCACAAC+AGG + chr8_1:75178094-75178113 None:intergenic 30.0%
! TCTTGCTAGATCTTTTTCTT+TGG + chr8_1:75178222-75178241 None:intergenic 30.0%
! TTTGGTCTTCAAGATATGTT+AGG - chr8_1:75178325-75178344 Msa1214510:CDS 30.0%
! TAACATTTTTCTCTCTTGCA+AGG + chr8_1:75178414-75178433 None:intergenic 30.0%
! AAGAGTGCATTCAATTCTAT+AGG - chr8_1:75178577-75178596 Msa1214510:CDS 30.0%
TAGAGAAAAAGCAAGAGAAA+GGG - chr8_1:75178600-75178619 Msa1214510:CDS 30.0%
AAGGACAAAGGAAAAAAAGA+AGG - chr8_1:75178630-75178649 Msa1214510:CDS 30.0%
TCAAAGCAATACAATGAAGA+AGG - chr8_1:75178682-75178701 Msa1214510:CDS 30.0%
CTTTGTTCATTATGAGCAAA+TGG - chr8_1:75178884-75178903 Msa1214510:CDS 30.0%
TTGAGAGAATTGAACATCAT+AGG + chr8_1:75178916-75178935 None:intergenic 30.0%
GCTTCATATATGTATATGCA+TGG + chr8_1:75178967-75178986 None:intergenic 30.0%
ACATAACATTCCAAAAGGAA+AGG + chr8_1:75179213-75179232 None:intergenic 30.0%
!!! TTTCCTTTTGGAATGTTATG+TGG - chr8_1:75179212-75179231 Msa1214510:CDS 30.0%
AACAGAAACAAACAAGAAAG+GGG + chr8_1:75179316-75179335 None:intergenic 30.0%
GAACAGAAACAAACAAGAAA+GGG + chr8_1:75179317-75179336 None:intergenic 30.0%
GTAAACTTGTACCAATTTCA+AGG + chr8_1:75179344-75179363 None:intergenic 30.0%
! CTGTTTGTTGAATACATATC+TGG + chr8_1:75177845-75177864 None:intergenic 30.0%
TTGCTTTCAAAGGAACAAAA+AGG + chr8_1:75177908-75177927 None:intergenic 30.0%
AATTGAAAGAAAGAGAAAGG+AGG + chr8_1:75177980-75177999 None:intergenic 30.0%
! TTGTTATTCATCATCACAAC+AGG + chr8_1:75178094-75178113 None:intergenic 30.0%
! TCTTGCTAGATCTTTTTCTT+TGG + chr8_1:75178222-75178241 None:intergenic 30.0%
! TTTGGTCTTCAAGATATGTT+AGG - chr8_1:75178325-75178344 Msa1214510:CDS 30.0%
! TAACATTTTTCTCTCTTGCA+AGG + chr8_1:75178414-75178433 None:intergenic 30.0%
! AAGAGTGCATTCAATTCTAT+AGG - chr8_1:75178577-75178596 Msa1214510:CDS 30.0%
TAGAGAAAAAGCAAGAGAAA+GGG - chr8_1:75178600-75178619 Msa1214510:CDS 30.0%
AAGGACAAAGGAAAAAAAGA+AGG - chr8_1:75178630-75178649 Msa1214510:CDS 30.0%
TCAAAGCAATACAATGAAGA+AGG - chr8_1:75178682-75178701 Msa1214510:CDS 30.0%
CTTTGTTCATTATGAGCAAA+TGG - chr8_1:75178884-75178903 Msa1214510:CDS 30.0%
TTGAGAGAATTGAACATCAT+AGG + chr8_1:75178916-75178935 None:intergenic 30.0%
GCTTCATATATGTATATGCA+TGG + chr8_1:75178967-75178986 None:intergenic 30.0%
ACATAACATTCCAAAAGGAA+AGG + chr8_1:75179213-75179232 None:intergenic 30.0%
!!! TTTCCTTTTGGAATGTTATG+TGG - chr8_1:75179212-75179231 Msa1214510:CDS 30.0%
AACAGAAACAAACAAGAAAG+GGG + chr8_1:75179316-75179335 None:intergenic 30.0%
GAACAGAAACAAACAAGAAA+GGG + chr8_1:75179317-75179336 None:intergenic 30.0%
GTAAACTTGTACCAATTTCA+AGG + chr8_1:75179344-75179363 None:intergenic 30.0%
ATCAAGACATGTACTAGCTA+GGG - chr8_1:75177725-75177744 Msa1214510:three_prime_UTR 35.0%
! TGCTAGGTTATTTCCATTGA+GGG + chr8_1:75177870-75177889 None:intergenic 35.0%
! ATGCTAGGTTATTTCCATTG+AGG + chr8_1:75177871-75177890 None:intergenic 35.0%
GTGATTTAGAGGAAAATGCT+AGG + chr8_1:75177886-75177905 None:intergenic 35.0%
GGAACAAAAAGGTGATTTAG+AGG + chr8_1:75177897-75177916 None:intergenic 35.0%
! TGAAGTTGAGTTGCTTTCAA+AGG + chr8_1:75177918-75177937 None:intergenic 35.0%
GGGAATTGAAAGAAAGAGAA+AGG + chr8_1:75177983-75178002 None:intergenic 35.0%
TGAAAGGGATCAATATCATC+AGG + chr8_1:75178013-75178032 None:intergenic 35.0%
AATCAACATCATGTTGTTGG+AGG + chr8_1:75178054-75178073 None:intergenic 35.0%
GGAAATCAACATCATGTTGT+TGG + chr8_1:75178057-75178076 None:intergenic 35.0%
TTATTCATCATCACAACAGG+TGG + chr8_1:75178091-75178110 None:intergenic 35.0%
GCTTTGATTGTTGAGTTTGA+AGG + chr8_1:75178139-75178158 None:intergenic 35.0%
!!! GAAGTTGCAAAGAGGTTTTT+TGG - chr8_1:75178307-75178326 Msa1214510:CDS 35.0%
TGAAAAAGCAAGCAAAACAG+TGG - chr8_1:75178351-75178370 Msa1214510:CDS 35.0%
GTTTGATCTCAAGTTTGGAT+TGG + chr8_1:75178390-75178409 None:intergenic 35.0%
! AAGGTGTTTGATCTCAAGTT+TGG + chr8_1:75178395-75178414 None:intergenic 35.0%
! GTTGAAGATGCACTTTTAGT+AGG + chr8_1:75178442-75178461 None:intergenic 35.0%
TCAACTTCTGAATGTACTGA+AGG - chr8_1:75178457-75178476 Msa1214510:CDS 35.0%
TCAAGCTTGGATAATGAAGT+TGG - chr8_1:75178484-75178503 Msa1214510:CDS 35.0%
GTAGAGAAAAAGCAAGAGAA+AGG - chr8_1:75178599-75178618 Msa1214510:CDS 35.0%
ATTCTTCCACACTTTCAAAG+GGG + chr8_1:75178732-75178751 None:intergenic 35.0%
GATTCTTCCACACTTTCAAA+GGG + chr8_1:75178733-75178752 None:intergenic 35.0%
AGATTCTTCCACACTTTCAA+AGG + chr8_1:75178734-75178753 None:intergenic 35.0%
AATCAGCTTCACATGATCAA+AGG + chr8_1:75178797-75178816 None:intergenic 35.0%
AAGCTACTCTCAAAAAGCTA+AGG - chr8_1:75178837-75178856 Msa1214510:CDS 35.0%
AGAGAATTGAACATCATAGG+TGG + chr8_1:75178913-75178932 None:intergenic 35.0%
TGAACAATTTCACCAATCCA+TGG - chr8_1:75179051-75179070 Msa1214510:CDS 35.0%
ACCAATCCATGGAAAAAACT+TGG - chr8_1:75179062-75179081 Msa1214510:CDS 35.0%
CCAATCCATGGAAAAAACTT+GGG - chr8_1:75179063-75179082 Msa1214510:CDS 35.0%
GGTAGCATGTTAATTAGATG+CGG + chr8_1:75179102-75179121 None:intergenic 35.0%
TCACCACATAACATTCCAAA+AGG + chr8_1:75179218-75179237 None:intergenic 35.0%
!!! TTTTGGAATGTTATGTGGTG+AGG - chr8_1:75179217-75179236 Msa1214510:CDS 35.0%
! GTGAGGTATCTTCAGAATTT+CGG - chr8_1:75179234-75179253 Msa1214510:exon 35.0%
GGAACAGAAACAAACAAGAA+AGG + chr8_1:75179318-75179337 None:intergenic 35.0%
ATCAAGACATGTACTAGCTA+GGG - chr8_1:75177725-75177744 Msa1214510:three_prime_UTR 35.0%
! TGCTAGGTTATTTCCATTGA+GGG + chr8_1:75177870-75177889 None:intergenic 35.0%
! ATGCTAGGTTATTTCCATTG+AGG + chr8_1:75177871-75177890 None:intergenic 35.0%
GTGATTTAGAGGAAAATGCT+AGG + chr8_1:75177886-75177905 None:intergenic 35.0%
GGAACAAAAAGGTGATTTAG+AGG + chr8_1:75177897-75177916 None:intergenic 35.0%
! TGAAGTTGAGTTGCTTTCAA+AGG + chr8_1:75177918-75177937 None:intergenic 35.0%
GGGAATTGAAAGAAAGAGAA+AGG + chr8_1:75177983-75178002 None:intergenic 35.0%
TGAAAGGGATCAATATCATC+AGG + chr8_1:75178013-75178032 None:intergenic 35.0%
AATCAACATCATGTTGTTGG+AGG + chr8_1:75178054-75178073 None:intergenic 35.0%
GGAAATCAACATCATGTTGT+TGG + chr8_1:75178057-75178076 None:intergenic 35.0%
TTATTCATCATCACAACAGG+TGG + chr8_1:75178091-75178110 None:intergenic 35.0%
GCTTTGATTGTTGAGTTTGA+AGG + chr8_1:75178139-75178158 None:intergenic 35.0%
!!! GAAGTTGCAAAGAGGTTTTT+TGG - chr8_1:75178307-75178326 Msa1214510:CDS 35.0%
TGAAAAAGCAAGCAAAACAG+TGG - chr8_1:75178351-75178370 Msa1214510:CDS 35.0%
GTTTGATCTCAAGTTTGGAT+TGG + chr8_1:75178390-75178409 None:intergenic 35.0%
! AAGGTGTTTGATCTCAAGTT+TGG + chr8_1:75178395-75178414 None:intergenic 35.0%
! GTTGAAGATGCACTTTTAGT+AGG + chr8_1:75178442-75178461 None:intergenic 35.0%
TCAACTTCTGAATGTACTGA+AGG - chr8_1:75178457-75178476 Msa1214510:CDS 35.0%
TCAAGCTTGGATAATGAAGT+TGG - chr8_1:75178484-75178503 Msa1214510:CDS 35.0%
GTAGAGAAAAAGCAAGAGAA+AGG - chr8_1:75178599-75178618 Msa1214510:CDS 35.0%
ATTCTTCCACACTTTCAAAG+GGG + chr8_1:75178732-75178751 None:intergenic 35.0%
GATTCTTCCACACTTTCAAA+GGG + chr8_1:75178733-75178752 None:intergenic 35.0%
AGATTCTTCCACACTTTCAA+AGG + chr8_1:75178734-75178753 None:intergenic 35.0%
AATCAGCTTCACATGATCAA+AGG + chr8_1:75178797-75178816 None:intergenic 35.0%
AAGCTACTCTCAAAAAGCTA+AGG - chr8_1:75178837-75178856 Msa1214510:CDS 35.0%
AGAGAATTGAACATCATAGG+TGG + chr8_1:75178913-75178932 None:intergenic 35.0%
TGAACAATTTCACCAATCCA+TGG - chr8_1:75179051-75179070 Msa1214510:CDS 35.0%
ACCAATCCATGGAAAAAACT+TGG - chr8_1:75179062-75179081 Msa1214510:CDS 35.0%
CCAATCCATGGAAAAAACTT+GGG - chr8_1:75179063-75179082 Msa1214510:CDS 35.0%
GGTAGCATGTTAATTAGATG+CGG + chr8_1:75179102-75179121 None:intergenic 35.0%
TCACCACATAACATTCCAAA+AGG + chr8_1:75179218-75179237 None:intergenic 35.0%
!!! TTTTGGAATGTTATGTGGTG+AGG - chr8_1:75179217-75179236 Msa1214510:CDS 35.0%
! GTGAGGTATCTTCAGAATTT+CGG - chr8_1:75179234-75179253 Msa1214510:exon 35.0%
GGAACAGAAACAAACAAGAA+AGG + chr8_1:75179318-75179337 None:intergenic 35.0%
CATCAAGACATGTACTAGCT+AGG - chr8_1:75177724-75177743 Msa1214510:three_prime_UTR 40.0%
TCAACAAACAGTTCCCTCAA+TGG - chr8_1:75177854-75177873 Msa1214510:three_prime_UTR 40.0%
AAGAAAGAGAAAGGAGGAAG+AGG + chr8_1:75177974-75177993 None:intergenic 40.0%
! ATTTGGTGGCTGTCTTGAAA+GGG + chr8_1:75178028-75178047 None:intergenic 40.0%
CATGTTGTTGGAGGAAGATT+TGG + chr8_1:75178045-75178064 None:intergenic 40.0%
!! ACAGTACTGATCATCATCAG+CGG - chr8_1:75178113-75178132 Msa1214510:intron 40.0%
TGGTAAAGGCAAAACTGTAG+TGG - chr8_1:75178186-75178205 Msa1214510:CDS 40.0%
! TGTCACTTGAAGTTGCAAAG+AGG - chr8_1:75178299-75178318 Msa1214510:CDS 40.0%
TACTGAAGGAGTTTCAAGCT+TGG - chr8_1:75178471-75178490 Msa1214510:CDS 40.0%
TTTCAAAGGGGTTCCAAATG+AGG + chr8_1:75178720-75178739 None:intergenic 40.0%
AGCTTCACATGATCAAAGGA+AGG + chr8_1:75178793-75178812 None:intergenic 40.0%
TCATGTGAAGCTGATTGATG+AGG - chr8_1:75178801-75178820 Msa1214510:CDS 40.0%
!!! CCCAAGTTTTTTCCATGGAT+TGG + chr8_1:75179066-75179085 None:intergenic 40.0%
ATCCATGGAAAAAACTTGGG+AGG - chr8_1:75179066-75179085 Msa1214510:CDS 40.0%
! AAAGGAGGTGGTGATGATAA+GGG + chr8_1:75179135-75179154 None:intergenic 40.0%
! AAAAGGAGGTGGTGATGATA+AGG + chr8_1:75179136-75179155 None:intergenic 40.0%
TCTGTTCCTGACCTTGAAAT+TGG - chr8_1:75179330-75179349 Msa1214510:five_prime_UTR 40.0%
CTTGTACCAATTTCAAGGTC+AGG + chr8_1:75179339-75179358 None:intergenic 40.0%
CATCAAGACATGTACTAGCT+AGG - chr8_1:75177724-75177743 Msa1214510:three_prime_UTR 40.0%
TCAACAAACAGTTCCCTCAA+TGG - chr8_1:75177854-75177873 Msa1214510:three_prime_UTR 40.0%
AAGAAAGAGAAAGGAGGAAG+AGG + chr8_1:75177974-75177993 None:intergenic 40.0%
! ATTTGGTGGCTGTCTTGAAA+GGG + chr8_1:75178028-75178047 None:intergenic 40.0%
CATGTTGTTGGAGGAAGATT+TGG + chr8_1:75178045-75178064 None:intergenic 40.0%
!! ACAGTACTGATCATCATCAG+CGG - chr8_1:75178113-75178132 Msa1214510:intron 40.0%
TGGTAAAGGCAAAACTGTAG+TGG - chr8_1:75178186-75178205 Msa1214510:CDS 40.0%
! TGTCACTTGAAGTTGCAAAG+AGG - chr8_1:75178299-75178318 Msa1214510:CDS 40.0%
TACTGAAGGAGTTTCAAGCT+TGG - chr8_1:75178471-75178490 Msa1214510:CDS 40.0%
TTTCAAAGGGGTTCCAAATG+AGG + chr8_1:75178720-75178739 None:intergenic 40.0%
AGCTTCACATGATCAAAGGA+AGG + chr8_1:75178793-75178812 None:intergenic 40.0%
TCATGTGAAGCTGATTGATG+AGG - chr8_1:75178801-75178820 Msa1214510:CDS 40.0%
!!! CCCAAGTTTTTTCCATGGAT+TGG + chr8_1:75179066-75179085 None:intergenic 40.0%
ATCCATGGAAAAAACTTGGG+AGG - chr8_1:75179066-75179085 Msa1214510:CDS 40.0%
! AAAGGAGGTGGTGATGATAA+GGG + chr8_1:75179135-75179154 None:intergenic 40.0%
! AAAAGGAGGTGGTGATGATA+AGG + chr8_1:75179136-75179155 None:intergenic 40.0%
TCTGTTCCTGACCTTGAAAT+TGG - chr8_1:75179330-75179349 Msa1214510:five_prime_UTR 40.0%
CTTGTACCAATTTCAAGGTC+AGG + chr8_1:75179339-75179358 None:intergenic 40.0%
AGAAAGGAGGAAGAGGAAAG+GGG + chr8_1:75177967-75177986 None:intergenic 45.0%
GAGAAAGGAGGAAGAGGAAA+GGG + chr8_1:75177968-75177987 None:intergenic 45.0%
AGAGAAAGGAGGAAGAGGAA+AGG + chr8_1:75177969-75177988 None:intergenic 45.0%
!! GATTTGGTGGCTGTCTTGAA+AGG + chr8_1:75178029-75178048 None:intergenic 45.0%
GTTGTTGGAGGAAGATTTGG+TGG + chr8_1:75178042-75178061 None:intergenic 45.0%
CAACAGGTGGATGATGAAGT+TGG + chr8_1:75178078-75178097 None:intergenic 45.0%
!! CGGTTTTGATCTTGCTGTGT+CGG + chr8_1:75178249-75178268 None:intergenic 45.0%
AGCAAGATCAAAACCGCGAA+AGG - chr8_1:75178253-75178272 Msa1214510:CDS 45.0%
TCAAAACCGCGAAAGGACTA+AGG - chr8_1:75178260-75178279 Msa1214510:CDS 45.0%
CAAAACCGCGAAAGGACTAA+GGG - chr8_1:75178261-75178280 Msa1214510:CDS 45.0%
AAGCAAGCAAAACAGTGGAC+TGG - chr8_1:75178356-75178375 Msa1214510:CDS 45.0%
CAAGAGAAAGGGCAAGAGAA+AGG - chr8_1:75178611-75178630 Msa1214510:CDS 45.0%
AAGGGCAAGAGAAAGGACAA+AGG - chr8_1:75178618-75178637 Msa1214510:CDS 45.0%
! TTGGAACCCCTTTGAAAGTG+TGG - chr8_1:75178723-75178742 Msa1214510:CDS 45.0%
! GAAAGTGTGGAAGAATCTGC+AGG - chr8_1:75178736-75178755 Msa1214510:CDS 45.0%
!!! TGCCTCCCAAGTTTTTTCCA+TGG + chr8_1:75179071-75179090 None:intergenic 45.0%
! GATGATAAGGGGTGCAAGTA+CGG + chr8_1:75179123-75179142 None:intergenic 45.0%
! AAGGAGGTGGTGATGATAAG+GGG + chr8_1:75179134-75179153 None:intergenic 45.0%
TGACTGACACAAAAAGGAGG+TGG + chr8_1:75179147-75179166 None:intergenic 45.0%
! ACCTCCTTTTTGTGTCAGTC+AGG - chr8_1:75179146-75179165 Msa1214510:CDS 45.0%
TGAGCCTGACTGACACAAAA+AGG + chr8_1:75179153-75179172 None:intergenic 45.0%
AGAAAGGAGGAAGAGGAAAG+GGG + chr8_1:75177967-75177986 None:intergenic 45.0%
GAGAAAGGAGGAAGAGGAAA+GGG + chr8_1:75177968-75177987 None:intergenic 45.0%
AGAGAAAGGAGGAAGAGGAA+AGG + chr8_1:75177969-75177988 None:intergenic 45.0%
!! GATTTGGTGGCTGTCTTGAA+AGG + chr8_1:75178029-75178048 None:intergenic 45.0%
GTTGTTGGAGGAAGATTTGG+TGG + chr8_1:75178042-75178061 None:intergenic 45.0%
CAACAGGTGGATGATGAAGT+TGG + chr8_1:75178078-75178097 None:intergenic 45.0%
!! CGGTTTTGATCTTGCTGTGT+CGG + chr8_1:75178249-75178268 None:intergenic 45.0%
AGCAAGATCAAAACCGCGAA+AGG - chr8_1:75178253-75178272 Msa1214510:CDS 45.0%
TCAAAACCGCGAAAGGACTA+AGG - chr8_1:75178260-75178279 Msa1214510:CDS 45.0%
CAAAACCGCGAAAGGACTAA+GGG - chr8_1:75178261-75178280 Msa1214510:CDS 45.0%
AAGCAAGCAAAACAGTGGAC+TGG - chr8_1:75178356-75178375 Msa1214510:CDS 45.0%
CAAGAGAAAGGGCAAGAGAA+AGG - chr8_1:75178611-75178630 Msa1214510:CDS 45.0%
AAGGGCAAGAGAAAGGACAA+AGG - chr8_1:75178618-75178637 Msa1214510:CDS 45.0%
! TTGGAACCCCTTTGAAAGTG+TGG - chr8_1:75178723-75178742 Msa1214510:CDS 45.0%
! GAAAGTGTGGAAGAATCTGC+AGG - chr8_1:75178736-75178755 Msa1214510:CDS 45.0%
!!! TGCCTCCCAAGTTTTTTCCA+TGG + chr8_1:75179071-75179090 None:intergenic 45.0%
! GATGATAAGGGGTGCAAGTA+CGG + chr8_1:75179123-75179142 None:intergenic 45.0%
! AAGGAGGTGGTGATGATAAG+GGG + chr8_1:75179134-75179153 None:intergenic 45.0%
TGACTGACACAAAAAGGAGG+TGG + chr8_1:75179147-75179166 None:intergenic 45.0%
! ACCTCCTTTTTGTGTCAGTC+AGG - chr8_1:75179146-75179165 Msa1214510:CDS 45.0%
TGAGCCTGACTGACACAAAA+AGG + chr8_1:75179153-75179172 None:intergenic 45.0%
GAAAGGAGGAAGAGGAAAGG+GGG + chr8_1:75177966-75177985 None:intergenic 50.0%
AATCTTCTTCCTGCAGCTGC+TGG - chr8_1:75178166-75178185 Msa1214510:CDS 50.0%
GCCTGACTGACACAAAAAGG+AGG + chr8_1:75179150-75179169 None:intergenic 50.0%
GAAAGGAGGAAGAGGAAAGG+GGG + chr8_1:75177966-75177985 None:intergenic 50.0%
AATCTTCTTCCTGCAGCTGC+TGG - chr8_1:75178166-75178185 Msa1214510:CDS 50.0%
GCCTGACTGACACAAAAAGG+AGG + chr8_1:75179150-75179169 None:intergenic 50.0%
CTTCCTGCAGCTGCTGGTAA+AGG - chr8_1:75178172-75178191 Msa1214510:CDS 55.0%
TTGCCTTTACCAGCAGCTGC+AGG + chr8_1:75178178-75178197 None:intergenic 55.0%
CCGATCCCTTAGTCCTTTCG+CGG + chr8_1:75178269-75178288 None:intergenic 55.0%
CCGCGAAAGGACTAAGGGAT+CGG - chr8_1:75178266-75178285 Msa1214510:CDS 55.0%
CTTCCTGCAGCTGCTGGTAA+AGG - chr8_1:75178172-75178191 Msa1214510:CDS 55.0%
TTGCCTTTACCAGCAGCTGC+AGG + chr8_1:75178178-75178197 None:intergenic 55.0%
CCGATCCCTTAGTCCTTTCG+CGG + chr8_1:75178269-75178288 None:intergenic 55.0%
CCGCGAAAGGACTAAGGGAT+CGG - chr8_1:75178266-75178285 Msa1214510:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 75177709 75179383 75177709 ID=Msa1214510;Name=Msa1214510
chr8_1 mRNA 75177709 75179383 75177709 ID=Msa1214510-mRNA-1;Parent=Msa1214510;Name=Msa1214510-mRNA-1;_AED=0.00;_eAED=0.00;_QI=139|1|1|1|0|0.5|2|275|388
chr8_1 exon 75177709 75178049 75177709 ID=Msa1214510-mRNA-1:exon:25811;Parent=Msa1214510-mRNA-1
chr8_1 exon 75178144 75179383 75178144 ID=Msa1214510-mRNA-1:exon:25810;Parent=Msa1214510-mRNA-1
chr8_1 five_prime_UTR 75179245 75179383 75179245 ID=Msa1214510-mRNA-1:five_prime_utr;Parent=Msa1214510-mRNA-1
chr8_1 CDS 75178144 75179244 75178144 ID=Msa1214510-mRNA-1:cds;Parent=Msa1214510-mRNA-1
chr8_1 CDS 75177984 75178049 75177984 ID=Msa1214510-mRNA-1:cds;Parent=Msa1214510-mRNA-1
chr8_1 three_prime_UTR 75177709 75177983 75177709 ID=Msa1214510-mRNA-1:three_prime_utr;Parent=Msa1214510-mRNA-1
Gene Sequence

>Msa1214510

ATGTATTCAACAAACAGTTCCCTCAATGGAAATAACCTAGCATTTTCCTCTAAATCACCTTTTTGTTCCTTTGAAAGCAACTCAACTTCATCAACAGATATTGAAAACAGTCATTCCCCCTTTCCTCTTCCTCCTTTCTCTTTCTTTCAATTCCCTAATTATCCTGATGATATTGATCCCTTTCAAGACAGCCACCAAATCTTCCTCCAACAACATGATGTTGATTTCCAACTTCATCATCCACCTGTTGTGATGATGAATAACAACAGTACTGATCATCATCAGCGGCCTTCAAACTCAACAATCAAAGCTAACAACAATCTTCTTCCTGCAGCTGCTGGTAAAGGCAAAACTGTAGTGGATCAGCAGATCCAAAGAAAAAGATCTAGCAAGAGAGACCGACACAGCAAGATCAAAACCGCGAAAGGACTAAGGGATCGGAGAATGAGATTGTCACTTGAAGTTGCAAAGAGGTTTTTTGGTCTTCAAGATATGTTAGGCTTTGAAAAAGCAAGCAAAACAGTGGACTGGTTGCTCAACCAATCCAAACTTGAGATCAAACACCTTGCAAGAGAGAAAAATGTTAATTTTCCTACTAAAAGTGCATCTTCAACTTCTGAATGTACTGAAGGAGTTTCAAGCTTGGATAATGAAGTTGGAAATCAAGAAGAACCACAACAACAAAAACTTGTTATGAAGAGAAGAAATAGTACTAATAAGGTTTGTAGAAAGAGTGCATTCAATTCTATAGGTAGAGAAAAAGCAAGAGAAAGGGCAAGAGAAAGGACAAAGGAAAAAAAGAAGGCTAGAACATCACTAGTTGATGAATCAAATTCAAAGCAATACAATGAAGAAGGAATAAAAACTAACCTCATTTGGAACCCCTTTGAAAGTGTGGAAGAATCTGCAGGTACACATCAGAGTACTCAAGATGTGAATCATCCTTCCTTTGATCATGTGAAGCTGATTGATGAGGCTGAAGTAGAAACAAGCTACTCTCAAAAAGCTAAGGAACAACATTCAGATGAAGATAATTCTTTGTTCATTATGAGCAAATGGAGTCCACCTATGATGTTCAATTCTCTCAATAACCTTCAACAACATCAATTTGAACAATTTCACCAATCCATGGAAAAAACTTGGGAGGCATACAACAACCGCATCTAA
Protein sequence

>Msa1214510

MYSTNSSLNGNNLAFSSKSPFCSFESNSTSSTDIENSHSPFPLPPFSFFQFPNYPDDIDPFQDSHQIFLQQHDVDFQLHHPPVVMMNNNSTDHHQRPSNSTIKANNNLLPAAAGKGKTVVDQQIQRKRSSKRDRHSKIKTAKGLRDRRMRLSLEVAKRFFGLQDMLGFEKASKTVDWLLNQSKLEIKHLAREKNVNFPTKSASSTSECTEGVSSLDNEVGNQEEPQQQKLVMKRRNSTNKVCRKSAFNSIGREKARERARERTKEKKKARTSLVDESNSKQYNEEGIKTNLIWNPFESVEESAGTHQSTQDVNHPSFDHVKLIDEAEVETSYSQKAKEQHSDEDNSLFIMSKWSPPMMFNSLNNLQQHQFEQFHQSMEKTWEAYNNRI*