AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1215560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1215560 MtrunA17_Chr4g0064671 96.429 84 3 0 6 89 169 252 1.85e-57 178
Msa1215560 MtrunA17_Chr1g0149991 54.808 104 43 1 6 109 452 551 4.01e-34 124
Msa1215560 MtrunA17_Chr6g0458441 39.744 78 43 4 10 83 349 426 6.21e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1215560 AT5G10790.2 50.000 92 43 1 3 91 319 410 7.03e-26 100
Msa1215560 AT5G10790.1 50.000 92 43 1 3 91 448 539 1.36e-25 100

Find 23 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTACAAGTGTGATGATTCT+TGG 0.387459 8_1:-76847805 Msa1215560:CDS
AAACATTGTGAGGGCTGCTC+AGG 0.399967 8_1:-76847765 Msa1215560:CDS
CATGGATCATTTGAAGCATC+TGG 0.409135 8_1:+76847917 None:intergenic
AAAATCATGGCATATCATTA+GGG 0.428866 8_1:+76847609 None:intergenic
AAGCAAAGCAACTGATAAAT+CGG 0.429703 8_1:-76847703 Msa1215560:CDS
GACATATGATGCCAATGGTT+TGG 0.451918 8_1:+76847652 None:intergenic
TAAATCGGTCGCCTCATGAT+TGG 0.463129 8_1:-76847688 Msa1215560:CDS
CTCATGATTGGCACAGGAGA+TGG 0.489277 8_1:-76847676 Msa1215560:CDS
CACTCTGGTAAGTTAGATGC+TGG 0.512954 8_1:-76847863 Msa1215560:CDS
GTACAAGTGTGATGATTCTT+GGG 0.516165 8_1:-76847804 Msa1215560:CDS
AAATTTGCAAAATGGTAAAG+AGG 0.517259 8_1:-76848442 None:intergenic
TCTGGCTCATCCCCATCAAA+AGG 0.519011 8_1:+76847935 None:intergenic
CAGGAGATGGTCCAAACCAT+TGG 0.530742 8_1:-76847663 Msa1215560:CDS
TTTGCTGTCGTCACTCACTC+TGG 0.539742 8_1:-76847878 Msa1215560:CDS
GGTCGCCTCATGATTGGCAC+AGG 0.544879 8_1:-76847682 Msa1215560:CDS
ATCTGCGATTAAGCAATCAA+TGG 0.573921 8_1:-76847826 Msa1215560:CDS
ACCATTGGCATCATATGTCA+TGG 0.588411 8_1:-76847648 Msa1215560:CDS
AACATTGTGAGGGCTGCTCA+GGG 0.594864 8_1:-76847764 Msa1215560:CDS
AACAATTCAAACTCAGAACA+TGG 0.598642 8_1:+76847899 None:intergenic
AAGTTGATGAAAACATTGTG+AGG 0.627661 8_1:-76847775 Msa1215560:CDS
CATCTCCTGTGCCAATCATG+AGG 0.630249 8_1:+76847677 None:intergenic
ACCATGACATATGATGCCAA+TGG 0.694643 8_1:+76847647 None:intergenic
AGTTGATGAAAACATTGTGA+GGG 0.699031 8_1:-76847774 Msa1215560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTTTCAAATTATTATTTG+GGG + chr8_1:76847758-76847777 None:intergenic 15.0%
!! CAACTTTCAAATTATTATTT+GGG + chr8_1:76847759-76847778 None:intergenic 15.0%
!! TCAACTTTCAAATTATTATT+TGG + chr8_1:76847760-76847779 None:intergenic 15.0%
!!! AAGTTGAATGATTATTTTTA+TGG - chr8_1:76847773-76847792 Msa1215560:CDS 15.0%
!! AACTTTCAAATTATTATTTG+GGG + chr8_1:76847758-76847777 None:intergenic 15.0%
!! CAACTTTCAAATTATTATTT+GGG + chr8_1:76847759-76847778 None:intergenic 15.0%
!! TCAACTTTCAAATTATTATT+TGG + chr8_1:76847760-76847779 None:intergenic 15.0%
!!! AAGTTGAATGATTATTTTTA+TGG - chr8_1:76847773-76847792 Msa1215560:CDS 15.0%
!! ATAGTCATTTAATGTCAAAA+TGG + chr8_1:76847800-76847819 None:intergenic 20.0%
!! GAAAAAACATGAAAAAAGAA+TGG - chr8_1:76847829-76847848 Msa1215560:CDS 20.0%
!!! ATAGAATATTTCCTTTTGAT+GGG - chr8_1:76848098-76848117 Msa1215560:intron 20.0%
!! ATAGTCATTTAATGTCAAAA+TGG + chr8_1:76847800-76847819 None:intergenic 20.0%
!! GAAAAAACATGAAAAAAGAA+TGG - chr8_1:76847829-76847848 Msa1215560:CDS 20.0%
!!! ATAGAATATTTCCTTTTGAT+GGG - chr8_1:76848098-76848117 Msa1215560:intron 20.0%
!! GATAGAATATTTCCTTTTGA+TGG - chr8_1:76848097-76848116 Msa1215560:intron 25.0%
!! TAGAATATTTCCTTTTGATG+GGG - chr8_1:76848099-76848118 Msa1215560:intron 25.0%
!! GATAGAATATTTCCTTTTGA+TGG - chr8_1:76848097-76848116 Msa1215560:intron 25.0%
!! TAGAATATTTCCTTTTGATG+GGG - chr8_1:76848099-76848118 Msa1215560:intron 25.0%
TAAAGAGGTTAGTGAAATAG+AGG - chr8_1:76847617-76847636 Msa1215560:CDS 30.0%
TTAACGGAAACATTAATGGA+GGG + chr8_1:76847661-76847680 None:intergenic 30.0%
TTTAACGGAAACATTAATGG+AGG + chr8_1:76847662-76847681 None:intergenic 30.0%
! CCTTTTAACGGAAACATTAA+TGG + chr8_1:76847665-76847684 None:intergenic 30.0%
CCATTAATGTTTCCGTTAAA+AGG - chr8_1:76847662-76847681 Msa1215560:CDS 30.0%
AATGTTTCCGTTAAAAGGTA+TGG - chr8_1:76847667-76847686 Msa1215560:CDS 30.0%
! AATTATTATTTGGGGCCAAA+TGG + chr8_1:76847750-76847769 None:intergenic 30.0%
ATTTCTCATCCTCAATCTTA+TGG - chr8_1:76847869-76847888 Msa1215560:CDS 30.0%
AGAGAAAATGAAATAGCCTT+CGG + chr8_1:76847960-76847979 None:intergenic 30.0%
AACAATTCAAACTCAGAACA+TGG + chr8_1:76848148-76848167 None:intergenic 30.0%
!! AAGTTGATGAAAACATTGTG+AGG - chr8_1:76848269-76848288 Msa1215560:intron 30.0%
!! AGTTGATGAAAACATTGTGA+GGG - chr8_1:76848270-76848289 Msa1215560:intron 30.0%
AAGCAAAGCAACTGATAAAT+CGG - chr8_1:76848341-76848360 Msa1215560:intron 30.0%
TAAAGAGGTTAGTGAAATAG+AGG - chr8_1:76847617-76847636 Msa1215560:CDS 30.0%
TTAACGGAAACATTAATGGA+GGG + chr8_1:76847661-76847680 None:intergenic 30.0%
TTTAACGGAAACATTAATGG+AGG + chr8_1:76847662-76847681 None:intergenic 30.0%
! CCTTTTAACGGAAACATTAA+TGG + chr8_1:76847665-76847684 None:intergenic 30.0%
CCATTAATGTTTCCGTTAAA+AGG - chr8_1:76847662-76847681 Msa1215560:CDS 30.0%
AATGTTTCCGTTAAAAGGTA+TGG - chr8_1:76847667-76847686 Msa1215560:CDS 30.0%
! AATTATTATTTGGGGCCAAA+TGG + chr8_1:76847750-76847769 None:intergenic 30.0%
ATTTCTCATCCTCAATCTTA+TGG - chr8_1:76847869-76847888 Msa1215560:CDS 30.0%
AGAGAAAATGAAATAGCCTT+CGG + chr8_1:76847960-76847979 None:intergenic 30.0%
AACAATTCAAACTCAGAACA+TGG + chr8_1:76848148-76848167 None:intergenic 30.0%
!! AAGTTGATGAAAACATTGTG+AGG - chr8_1:76848269-76848288 Msa1215560:intron 30.0%
!! AGTTGATGAAAACATTGTGA+GGG - chr8_1:76848270-76848289 Msa1215560:intron 30.0%
AAGCAAAGCAACTGATAAAT+CGG - chr8_1:76848341-76848360 Msa1215560:intron 30.0%
!! TAATGGAGGGACTATTTTGA+TGG + chr8_1:76847648-76847667 None:intergenic 35.0%
GAGAAAATGAAATAGCCTTC+GGG + chr8_1:76847959-76847978 None:intergenic 35.0%
CAATCACAATAAAGCTTGTG+CGG + chr8_1:76848018-76848037 None:intergenic 35.0%
ATCTGCGATTAAGCAATCAA+TGG - chr8_1:76848218-76848237 Msa1215560:intron 35.0%
GTACAAGTGTGATGATTCTT+GGG - chr8_1:76848240-76848259 Msa1215560:intron 35.0%
!! TAATGGAGGGACTATTTTGA+TGG + chr8_1:76847648-76847667 None:intergenic 35.0%
GAGAAAATGAAATAGCCTTC+GGG + chr8_1:76847959-76847978 None:intergenic 35.0%
CAATCACAATAAAGCTTGTG+CGG + chr8_1:76848018-76848037 None:intergenic 35.0%
ATCTGCGATTAAGCAATCAA+TGG - chr8_1:76848218-76848237 Msa1215560:intron 35.0%
GTACAAGTGTGATGATTCTT+GGG - chr8_1:76848240-76848259 Msa1215560:intron 35.0%
! TTCCGTTAAAAGGTATGGAG+TGG - chr8_1:76847672-76847691 Msa1215560:CDS 40.0%
! TGCCACTCCATACCTTTTAA+CGG + chr8_1:76847677-76847696 None:intergenic 40.0%
! CACATCAGTATCGAACCATT+TGG - chr8_1:76847732-76847751 Msa1215560:CDS 40.0%
CATCCTCAATCTTATGGAGT+CGG - chr8_1:76847875-76847894 Msa1215560:CDS 40.0%
! AAACCGACTCCATAAGATTG+AGG + chr8_1:76847881-76847900 None:intergenic 40.0%
TCTCTCAGGACAGTTTGTAT+CGG + chr8_1:76847934-76847953 None:intergenic 40.0%
TAAAGCTTGTGCGGAATTCA+CGG + chr8_1:76848009-76848028 None:intergenic 40.0%
!! CGCACAAGCTTTATTGTGAT+TGG - chr8_1:76848016-76848035 Msa1215560:intron 40.0%
! CATGGATCATTTGAAGCATC+TGG + chr8_1:76848130-76848149 None:intergenic 40.0%
GGTACAAGTGTGATGATTCT+TGG - chr8_1:76848239-76848258 Msa1215560:intron 40.0%
GACATATGATGCCAATGGTT+TGG + chr8_1:76848395-76848414 None:intergenic 40.0%
ACCATTGGCATCATATGTCA+TGG - chr8_1:76848396-76848415 Msa1215560:intron 40.0%
ACCATGACATATGATGCCAA+TGG + chr8_1:76848400-76848419 None:intergenic 40.0%
! TTCCGTTAAAAGGTATGGAG+TGG - chr8_1:76847672-76847691 Msa1215560:CDS 40.0%
! TGCCACTCCATACCTTTTAA+CGG + chr8_1:76847677-76847696 None:intergenic 40.0%
! CACATCAGTATCGAACCATT+TGG - chr8_1:76847732-76847751 Msa1215560:CDS 40.0%
CATCCTCAATCTTATGGAGT+CGG - chr8_1:76847875-76847894 Msa1215560:CDS 40.0%
! AAACCGACTCCATAAGATTG+AGG + chr8_1:76847881-76847900 None:intergenic 40.0%
TCTCTCAGGACAGTTTGTAT+CGG + chr8_1:76847934-76847953 None:intergenic 40.0%
TAAAGCTTGTGCGGAATTCA+CGG + chr8_1:76848009-76848028 None:intergenic 40.0%
!! CGCACAAGCTTTATTGTGAT+TGG - chr8_1:76848016-76848035 Msa1215560:intron 40.0%
! CATGGATCATTTGAAGCATC+TGG + chr8_1:76848130-76848149 None:intergenic 40.0%
GGTACAAGTGTGATGATTCT+TGG - chr8_1:76848239-76848258 Msa1215560:intron 40.0%
GACATATGATGCCAATGGTT+TGG + chr8_1:76848395-76848414 None:intergenic 40.0%
ACCATTGGCATCATATGTCA+TGG - chr8_1:76848396-76848415 Msa1215560:intron 40.0%
ACCATGACATATGATGCCAA+TGG + chr8_1:76848400-76848419 None:intergenic 40.0%
GGTATGGAGTGGCACATTAA+CGG - chr8_1:76847683-76847702 Msa1215560:CDS 45.0%
AGTGGCACATTAACGGTTTG+TGG - chr8_1:76847690-76847709 Msa1215560:CDS 45.0%
GTTTGTGGTGACACTCAACT+TGG - chr8_1:76847705-76847724 Msa1215560:CDS 45.0%
CTCTCAGGACAGTTTGTATC+GGG + chr8_1:76847933-76847952 None:intergenic 45.0%
AAAGCTTGTGCGGAATTCAC+GGG + chr8_1:76848008-76848027 None:intergenic 45.0%
! GCTTTATTGTGATTGGCTGC+TGG - chr8_1:76848023-76848042 Msa1215560:intron 45.0%
! TGATTGGCTGCTGGTGATTT+CGG - chr8_1:76848032-76848051 Msa1215560:intron 45.0%
TGGTGATTTCGGGATAAGCT+CGG - chr8_1:76848043-76848062 Msa1215560:intron 45.0%
! AAGCTCGGGACGTTTTCTTT+CGG - chr8_1:76848058-76848077 Msa1215560:intron 45.0%
CACTCTGGTAAGTTAGATGC+TGG - chr8_1:76848181-76848200 Msa1215560:intron 45.0%
TAAATCGGTCGCCTCATGAT+TGG - chr8_1:76848356-76848375 Msa1215560:intron 45.0%
GGTATGGAGTGGCACATTAA+CGG - chr8_1:76847683-76847702 Msa1215560:CDS 45.0%
AGTGGCACATTAACGGTTTG+TGG - chr8_1:76847690-76847709 Msa1215560:CDS 45.0%
GTTTGTGGTGACACTCAACT+TGG - chr8_1:76847705-76847724 Msa1215560:CDS 45.0%
CTCTCAGGACAGTTTGTATC+GGG + chr8_1:76847933-76847952 None:intergenic 45.0%
AAAGCTTGTGCGGAATTCAC+GGG + chr8_1:76848008-76848027 None:intergenic 45.0%
! GCTTTATTGTGATTGGCTGC+TGG - chr8_1:76848023-76848042 Msa1215560:intron 45.0%
! TGATTGGCTGCTGGTGATTT+CGG - chr8_1:76848032-76848051 Msa1215560:intron 45.0%
TGGTGATTTCGGGATAAGCT+CGG - chr8_1:76848043-76848062 Msa1215560:intron 45.0%
! AAGCTCGGGACGTTTTCTTT+CGG - chr8_1:76848058-76848077 Msa1215560:intron 45.0%
CACTCTGGTAAGTTAGATGC+TGG - chr8_1:76848181-76848200 Msa1215560:intron 45.0%
TAAATCGGTCGCCTCATGAT+TGG - chr8_1:76848356-76848375 Msa1215560:intron 45.0%
ATAGCCTTCGGGAATCTCTC+AGG + chr8_1:76847948-76847967 None:intergenic 50.0%
CGGGAGAGAGAGGGATAAAA+CGG + chr8_1:76847989-76848008 None:intergenic 50.0%
! GATTGGCTGCTGGTGATTTC+GGG - chr8_1:76848033-76848052 Msa1215560:intron 50.0%
GGTGATTTCGGGATAAGCTC+GGG - chr8_1:76848044-76848063 Msa1215560:intron 50.0%
! AGCTCGGGACGTTTTCTTTC+GGG - chr8_1:76848059-76848078 Msa1215560:intron 50.0%
! TCTGGCTCATCCCCATCAAA+AGG + chr8_1:76848112-76848131 None:intergenic 50.0%
! TTTGCTGTCGTCACTCACTC+TGG - chr8_1:76848166-76848185 Msa1215560:intron 50.0%
AAACATTGTGAGGGCTGCTC+AGG - chr8_1:76848279-76848298 Msa1215560:intron 50.0%
AACATTGTGAGGGCTGCTCA+GGG - chr8_1:76848280-76848299 Msa1215560:intron 50.0%
CATCTCCTGTGCCAATCATG+AGG + chr8_1:76848370-76848389 None:intergenic 50.0%
CTCATGATTGGCACAGGAGA+TGG - chr8_1:76848368-76848387 Msa1215560:intron 50.0%
CAGGAGATGGTCCAAACCAT+TGG - chr8_1:76848381-76848400 Msa1215560:intron 50.0%
ATAGCCTTCGGGAATCTCTC+AGG + chr8_1:76847948-76847967 None:intergenic 50.0%
CGGGAGAGAGAGGGATAAAA+CGG + chr8_1:76847989-76848008 None:intergenic 50.0%
! GATTGGCTGCTGGTGATTTC+GGG - chr8_1:76848033-76848052 Msa1215560:intron 50.0%
GGTGATTTCGGGATAAGCTC+GGG - chr8_1:76848044-76848063 Msa1215560:intron 50.0%
! AGCTCGGGACGTTTTCTTTC+GGG - chr8_1:76848059-76848078 Msa1215560:intron 50.0%
! TCTGGCTCATCCCCATCAAA+AGG + chr8_1:76848112-76848131 None:intergenic 50.0%
! TTTGCTGTCGTCACTCACTC+TGG - chr8_1:76848166-76848185 Msa1215560:intron 50.0%
AAACATTGTGAGGGCTGCTC+AGG - chr8_1:76848279-76848298 Msa1215560:intron 50.0%
AACATTGTGAGGGCTGCTCA+GGG - chr8_1:76848280-76848299 Msa1215560:intron 50.0%
CATCTCCTGTGCCAATCATG+AGG + chr8_1:76848370-76848389 None:intergenic 50.0%
CTCATGATTGGCACAGGAGA+TGG - chr8_1:76848368-76848387 Msa1215560:intron 50.0%
CAGGAGATGGTCCAAACCAT+TGG - chr8_1:76848381-76848400 Msa1215560:intron 50.0%
CTGTCCTGAGAGATTCCCGA+AGG - chr8_1:76847941-76847960 Msa1215560:CDS 55.0%
CGGAATTCACGGGAGAGAGA+GGG + chr8_1:76847998-76848017 None:intergenic 55.0%
CTGTCCTGAGAGATTCCCGA+AGG - chr8_1:76847941-76847960 Msa1215560:CDS 55.0%
CGGAATTCACGGGAGAGAGA+GGG + chr8_1:76847998-76848017 None:intergenic 55.0%
GCGGAATTCACGGGAGAGAG+AGG + chr8_1:76847999-76848018 None:intergenic 60.0%
GGTCGCCTCATGATTGGCAC+AGG - chr8_1:76848362-76848381 Msa1215560:intron 60.0%
GCGGAATTCACGGGAGAGAG+AGG + chr8_1:76847999-76848018 None:intergenic 60.0%
GGTCGCCTCATGATTGGCAC+AGG - chr8_1:76848362-76848381 Msa1215560:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 76847613 76848453 76847613 ID=Msa1215560;Name=Msa1215560
chr8_1 mRNA 76847613 76848453 76847613 ID=Msa1215560-mRNA-1;Parent=Msa1215560;Name=Msa1215560-mRNA-1;_AED=0.54;_eAED=0.54;_QI=0|0|0|0.5|0|0|2|0|124
chr8_1 exon 76848443 76848453 76848443 ID=Msa1215560-mRNA-1:exon:26494;Parent=Msa1215560-mRNA-1
chr8_1 exon 76847613 76847976 76847613 ID=Msa1215560-mRNA-1:exon:26493;Parent=Msa1215560-mRNA-1
chr8_1 CDS 76848443 76848453 76848443 ID=Msa1215560-mRNA-1:cds;Parent=Msa1215560-mRNA-1
chr8_1 CDS 76847613 76847976 76847613 ID=Msa1215560-mRNA-1:cds;Parent=Msa1215560-mRNA-1
Gene Sequence

>Msa1215560

ATGGTAAAGAGCACAGCTGATAGAATATTTCCTTTTGATGGGGATGAGCCAGATGCTTCAAATGATCCATGTTCTGAGTTTGAATTGTTTGCTGTCGTCACTCACTCTGGTAAGTTAGATGCTGGTCATTATGTGACATATCTGCGATTAAGCAATCAATGGTACAAGTGTGATGATTCTTGGGTTACTCAAGTTGATGAAAACATTGTGAGGGCTGCTCAGGGTTACATGATGTTCTATGTACAGAAGATGCTTTACTATAAAGCAAAGCAACTGATAAATCGGTCGCCTCATGATTGGCACAGGAGATGGTCCAAACCATTGGCATCATATGTCATGGTCAATATCAGTTTTCACCCTAATGATATGCCATGA

Protein sequence

>Msa1215560

MVKSTADRIFPFDGDEPDASNDPCSEFELFAVVTHSGKLDAGHYVTYLRLSNQWYKCDDSWVTQVDENIVRAAQGYMMFYVQKMLYYKAKQLINRSPHDWHRRWSKPLASYVMVNISFHPNDMP*