AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1216200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1216200 MtrunA17_Chr5g0448391 70.833 48 14 0 88 135 26 73 1.29e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 108 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGAATAAACTCTTTATTT+TGG 0.185794 8_1:-77846457 None:intergenic
TTGCAGGTGCCTGGGGTTTC+CGG 0.195942 8_1:+77845890 Msa1216200:intron
TGCTACTTTGGTGGAGTTCC+TGG 0.234539 8_1:+77846085 Msa1216200:three_prime_UTR
TTGGACAGTTTCGACGTTTC+GGG 0.240386 8_1:+77845755 Msa1216200:intron
CGTAGGTTATGGCATAGTTA+TGG 0.291956 8_1:+77845932 Msa1216200:exon
TGAATTCCGTAGGTCATCTT+TGG 0.299400 8_1:+77845958 Msa1216200:three_prime_UTR
TTTGGACAGTTTCGACGTTT+CGG 0.305938 8_1:+77845754 Msa1216200:intron
ACAGTATCGAACCATCATTA+TGG 0.315837 8_1:-77846051 None:intergenic
ATCCTTGCATCATAATTGAT+TGG 0.323481 8_1:+77846265 Msa1216200:three_prime_UTR
GCACTCTTAGTAAATCTAAA+AGG 0.330629 8_1:+77846503 Msa1216200:three_prime_UTR
GGTGTTCCGCCTATAGAAAT+AGG 0.332859 8_1:+77846351 Msa1216200:three_prime_UTR
TTATGGCCGTTGCAGGTGCC+TGG 0.349373 8_1:+77845881 Msa1216200:intron
AGCTTCCATTACGGAGGGGC+TGG 0.354407 8_1:-77843387 None:intergenic
TGCCAAACCCTCCATTCTAT+TGG 0.361812 8_1:+77843359 Msa1216200:CDS
ACATGAGGTGGCGGCTCTGG+TGG 0.366154 8_1:-77843634 None:intergenic
AACACCGTAAATAATCAAAA+AGG 0.368236 8_1:-77846334 None:intergenic
GTAGGTTATGGCATAGTTAT+GGG 0.369274 8_1:+77845933 Msa1216200:exon
CATGAGGTGGCGGCTCTGGT+GGG 0.376867 8_1:-77843633 None:intergenic
AGATTTACTAAGAGTGCATC+AGG 0.390361 8_1:-77846497 None:intergenic
TGGATTTCAGTGTCATATCC+AGG 0.391609 8_1:+77845978 Msa1216200:three_prime_UTR
CTCCAATAGAATGGAGGGTT+TGG 0.398970 8_1:-77843361 None:intergenic
TATGATGCAAGGATATAAAA+AGG 0.399801 8_1:-77846256 None:intergenic
TCCTTGCATCATAATTGATT+GGG 0.403311 8_1:+77846266 Msa1216200:three_prime_UTR
TTCACATGAGGTGGCGGCTC+TGG 0.404013 8_1:-77843637 None:intergenic
CAGTATCGAACCATCATTAT+GGG 0.413109 8_1:-77846050 None:intergenic
CAAGGCTGTTGCTACTTTGG+TGG 0.414565 8_1:+77846076 Msa1216200:three_prime_UTR
AGCTTATTTCCCCTCCGTAA+TGG 0.419483 8_1:+77843406 Msa1216200:CDS
TTACAAGGCTGTTGCTACTT+TGG 0.420680 8_1:+77846073 Msa1216200:three_prime_UTR
AAGAGTTTATTCCTTGTAGT+AGG 0.425407 8_1:+77846465 Msa1216200:three_prime_UTR
TGAGGTGGCGGCTCTGGTGG+GGG 0.430876 8_1:-77843631 None:intergenic
TTTCGACGTTTCGGGCTGTA+GGG 0.442256 8_1:+77845763 Msa1216200:CDS
AAAACACGCCCTATTTCTAT+AGG 0.447088 8_1:-77846360 None:intergenic
GGATCTCTCTCGTTTGCTCT+TGG 0.451038 8_1:+77843568 Msa1216200:CDS
CAGAGATTGTGTTTCTCAGT+TGG 0.454829 8_1:-77843594 None:intergenic
ACAGACGGCGATGGCAGATC+CGG 0.456209 8_1:-77843682 None:intergenic
TATGGCCGTTGCAGGTGCCT+GGG 0.457031 8_1:+77845882 Msa1216200:intron
CTGAGAATCGATGGTGGATA+AGG 0.457251 8_1:-77843724 None:intergenic
AGAGATTGTGTTTCTCAGTT+GGG 0.458081 8_1:-77843593 None:intergenic
CACAAAACCCCTTCAGCAGC+AGG 0.462304 8_1:+77843547 Msa1216200:CDS
AGTTCTCCCCTTCTCATTGA+AGG 0.469004 8_1:+77843430 Msa1216200:CDS
ATGAGGTGGCGGCTCTGGTG+GGG 0.469088 8_1:-77843632 None:intergenic
GTGTTCCGCCTATAGAAATA+GGG 0.481864 8_1:+77846352 Msa1216200:three_prime_UTR
GCTTCCATTACGGAGGGGCT+GGG 0.485234 8_1:-77843386 None:intergenic
GTTTCGACGTTTCGGGCTGT+AGG 0.489022 8_1:+77845762 Msa1216200:intron
TGTGTGTGTGGCAGCTCTGT+TGG 0.497376 8_1:+77846130 Msa1216200:three_prime_UTR
AGATGAAGTGTGGACTGAAA+GGG 0.498409 8_1:-77843474 None:intergenic
TGCCTGGGGTTTCCGGATTG+TGG 0.502441 8_1:+77845897 Msa1216200:CDS
AAAAGTACCTTCAATGAGAA+GGG 0.512331 8_1:-77843437 None:intergenic
TATCCAGGGGCAGATGTATG+TGG 0.517377 8_1:+77845993 Msa1216200:three_prime_UTR
AGAACCCAGCCCCTCCGTAA+TGG 0.519154 8_1:+77843382 Msa1216200:CDS
CACCACAATCCGGAAACCCC+AGG 0.521302 8_1:-77845899 None:intergenic
AAGATGAAGTGTGGACTGAA+AGG 0.522770 8_1:-77843475 None:intergenic
ATCTGTTTCACATGAGGTGG+CGG 0.524655 8_1:-77843643 None:intergenic
GGCTGGGTTCTCCAATAGAA+TGG 0.531167 8_1:-77843370 None:intergenic
AAAACTGCAGCACCACAATC+CGG 0.531873 8_1:-77845909 None:intergenic
GGGGAAATAAGCTTCCATTA+CGG 0.533732 8_1:-77843396 None:intergenic
GGATTTCAGTGTCATATCCA+GGG 0.542599 8_1:+77845979 Msa1216200:three_prime_UTR
TAAAACAAAAGCTTAACAGC+TGG 0.545088 8_1:-77846223 None:intergenic
GCTTACGCGGAGGCCGTACA+AGG 0.546430 8_1:-77843747 None:intergenic
ACCCAATCAATTATGATGCA+AGG 0.548459 8_1:-77846267 None:intergenic
GAAGGGGAGAACTTCCATTA+CGG 0.548862 8_1:-77843420 None:intergenic
CATCGATTCTCAGCCTTGTA+CGG 0.550510 8_1:+77843734 Msa1216200:CDS
GAGAGATCCTGCTGCTGAAG+GGG 0.551750 8_1:-77843554 None:intergenic
AGAGAGATCCTGCTGCTGAA+GGG 0.552367 8_1:-77843555 None:intergenic
GATCATCCACTTTGGTCCAC+CGG 0.552525 8_1:+77843663 Msa1216200:CDS
GTTAGGCAGAGGGGTATGCA+TGG 0.553611 8_1:+77846183 Msa1216200:three_prime_UTR
AGATGTATGTGGGAGCTATG+TGG 0.560753 8_1:+77846004 Msa1216200:three_prime_UTR
CTCCCACATACATCTGCCCC+TGG 0.561250 8_1:-77845996 None:intergenic
GAGAGAGATCCTGCTGCTGA+AGG 0.561653 8_1:-77843556 None:intergenic
TTCGACGTTTCGGGCTGTAG+GGG 0.568472 8_1:+77845764 Msa1216200:CDS
GTGAAACAGATCATCCACTT+TGG 0.571766 8_1:+77843655 Msa1216200:CDS
GTGATTGTGGTGACATGGTA+AGG 0.574127 8_1:-77843498 None:intergenic
GACGGCGATGGCAGATCCGG+TGG 0.575149 8_1:-77843679 None:intergenic
GATGTATGTGGGAGCTATGT+GGG 0.577005 8_1:+77846005 Msa1216200:three_prime_UTR
GGGTTCTCCAATAGAATGGA+GGG 0.585537 8_1:-77843366 None:intergenic
TCGACGTTTCGGGCTGTAGG+GGG 0.590638 8_1:+77845765 Msa1216200:CDS
TCTGGATCGGTGGCAGCAAG+AGG 0.593704 8_1:-77843522 None:intergenic
GATGAAGTGTGGACTGAAAG+GGG 0.594339 8_1:-77843473 None:intergenic
CGTACAAGGCTGAGAATCGA+TGG 0.594576 8_1:-77843733 None:intergenic
TGAAATCCAAAGATGACCTA+CGG 0.599719 8_1:-77845964 None:intergenic
ATGGCCGTTGCAGGTGCCTG+GGG 0.608536 8_1:+77845883 Msa1216200:intron
AAGACAAGCTTCAAAACAGA+CGG 0.609330 8_1:-77843697 None:intergenic
ATCCAGGGGCAGATGTATGT+GGG 0.611379 8_1:+77845994 Msa1216200:three_prime_UTR
AAATAAGCTTCCATTACGGA+GGG 0.614463 8_1:-77843392 None:intergenic
GGAAAATTGATCCTACTACA+AGG 0.616080 8_1:-77846476 None:intergenic
AGCTTCAAAACAGACGGCGA+TGG 0.617315 8_1:-77843691 None:intergenic
GATGGTTCGATACTGTTACA+AGG 0.624697 8_1:+77846058 Msa1216200:three_prime_UTR
GGGAGAACTTCCATTACGGA+GGG 0.626059 8_1:-77843416 None:intergenic
GATTTCAGTGTCATATCCAG+GGG 0.627782 8_1:+77845980 Msa1216200:three_prime_UTR
GTTATGGGTCTGAATTCCGT+AGG 0.634027 8_1:+77845948 Msa1216200:three_prime_UTR
GGCAGCAAGAGGTGTGATTG+TGG 0.634747 8_1:-77843511 None:intergenic
ATGATCTGTTTCACATGAGG+TGG 0.635789 8_1:-77843646 None:intergenic
AAAGTACCTTCAATGAGAAG+GGG 0.639653 8_1:-77843436 None:intergenic
GCAGATCCGGTGGACCAAAG+TGG 0.640804 8_1:-77843669 None:intergenic
TGGGTTCTCCAATAGAATGG+AGG 0.642827 8_1:-77843367 None:intergenic
TCATTATGGGGAAAAGCATG+TGG 0.642985 8_1:-77846037 None:intergenic
GAAATAAGCTTCCATTACGG+AGG 0.656243 8_1:-77843393 None:intergenic
AGTATCGAACCATCATTATG+GGG 0.669754 8_1:-77846049 None:intergenic
GAGGTGTGATTGTGGTGACA+TGG 0.670312 8_1:-77843503 None:intergenic
GGGGAGAACTTCCATTACGG+AGG 0.671617 8_1:-77843417 None:intergenic
ACACGCCCTATTTCTATAGG+CGG 0.674415 8_1:-77846357 None:intergenic
ATGAAGTGTGGACTGAAAGG+GGG 0.674586 8_1:-77843472 None:intergenic
GAGCTAATATGTTGTGTGTG+TGG 0.674657 8_1:+77846118 Msa1216200:three_prime_UTR
AATAAGCTTCCATTACGGAG+GGG 0.693424 8_1:-77843391 None:intergenic
ACAAGGCTGAGAATCGATGG+TGG 0.697728 8_1:-77843730 None:intergenic
ATGGTAAGGAAGATGAAGTG+TGG 0.708922 8_1:-77843484 None:intergenic
GGAGAACTTCCATTACGGAG+GGG 0.711943 8_1:-77843415 None:intergenic
TGGATGATCTGTTTCACATG+AGG 0.715310 8_1:-77843649 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATAATTGCCAAATAAA+TGG - chr8_1:77844966-77844985 None:intergenic 15.0%
!! TGTTTGAAGATTAATTATTT+AGG + chr8_1:77845651-77845670 Msa1216200:intron 15.0%
!! GTTTGAAGATTAATTATTTA+GGG + chr8_1:77845652-77845671 Msa1216200:intron 15.0%
!!! CATATTGTTAATTTTGTATT+TGG + chr8_1:77846550-77846569 Msa1216200:three_prime_UTR 15.0%
!!! TTTTCAGAATTCTTAAAACT+AGG - chr8_1:77844647-77844666 None:intergenic 20.0%
!!! GTTTTGGAATTTGTATAATA+CGG - chr8_1:77844850-77844869 None:intergenic 20.0%
!! TGAATCTCTTATTAAAAGAA+GGG - chr8_1:77844872-77844891 None:intergenic 20.0%
!! TTGAATCTCTTATTAAAAGA+AGG - chr8_1:77844873-77844892 None:intergenic 20.0%
!! TGAATCAAAATGGAAAATAA+AGG - chr8_1:77844906-77844925 None:intergenic 20.0%
!!! ATTCAATTATCATTTTGAGA+AGG + chr8_1:77845456-77845475 Msa1216200:intron 20.0%
!!! TGTTCCTTTTTGATTATTTA+CGG + chr8_1:77846330-77846349 Msa1216200:three_prime_UTR 20.0%
!!! AAGGAATAAACTCTTTATTT+TGG - chr8_1:77846460-77846479 None:intergenic 20.0%
! GACACAAATCAATTGATAAA+GGG - chr8_1:77844067-77844086 None:intergenic 25.0%
!!! TCTTTTTTCCTATCCAATTA+CGG - chr8_1:77844161-77844180 None:intergenic 25.0%
!! TTGTTGATGAATGAGTTATT+AGG + chr8_1:77844225-77844244 Msa1216200:intron 25.0%
! ATGAATGAGTTATTAGGTTA+TGG + chr8_1:77844231-77844250 Msa1216200:intron 25.0%
! AGTTATTAGGTTATGGAATT+TGG + chr8_1:77844238-77844257 Msa1216200:intron 25.0%
! TCAAAAACACCTATTAAAAC+AGG - chr8_1:77844309-77844328 None:intergenic 25.0%
! ATAAACAAGAGAGAATTACA+AGG - chr8_1:77844387-77844406 None:intergenic 25.0%
! TAGAATAGTTTACTTGGAAT+TGG - chr8_1:77844528-77844547 None:intergenic 25.0%
! TTGCTATAGAATAGTTTACT+TGG - chr8_1:77844534-77844553 None:intergenic 25.0%
! AATTGCATAATAGCATCAAA+CGG - chr8_1:77844741-77844760 None:intergenic 25.0%
!! TCTTATTAAAAGAAGGGTTT+TGG - chr8_1:77844866-77844885 None:intergenic 25.0%
!! TTTCCCTTTTTCCATTTATT+TGG + chr8_1:77844952-77844971 Msa1216200:intron 25.0%
! TTGCCAAATAAATGGAAAAA+GGG - chr8_1:77844958-77844977 None:intergenic 25.0%
! ATTGCCAAATAAATGGAAAA+AGG - chr8_1:77844959-77844978 None:intergenic 25.0%
! TGGCAATTATTAATTCTTGT+TGG + chr8_1:77844972-77844991 Msa1216200:intron 25.0%
! CTCAAAAGAATAAGAAAAAC+AGG - chr8_1:77845113-77845132 None:intergenic 25.0%
! AAAACACAAAATAACATCAG+TGG - chr8_1:77845224-77845243 None:intergenic 25.0%
!! TCTTTTGTGTATATGTAATG+AGG + chr8_1:77845262-77845281 Msa1216200:intron 25.0%
! ATGTTACAAGGCATAATTAT+AGG - chr8_1:77845300-77845319 None:intergenic 25.0%
! TATAATTATGCCTTGTAACA+TGG + chr8_1:77845299-77845318 Msa1216200:intron 25.0%
! AAAATATTTGCCATGTTACA+AGG - chr8_1:77845312-77845331 None:intergenic 25.0%
!! TTGTAACATGGCAAATATTT+TGG + chr8_1:77845311-77845330 Msa1216200:intron 25.0%
!! CAGTATAATGATTTTGTGTT+AGG + chr8_1:77845393-77845412 Msa1216200:intron 25.0%
! TACACATAACACTAAAAAAG+AGG - chr8_1:77845500-77845519 None:intergenic 25.0%
! TATGATGCAAGGATATAAAA+AGG - chr8_1:77846259-77846278 None:intergenic 25.0%
! ATCAAAAAGGAACAAAAATC+CGG - chr8_1:77846324-77846343 None:intergenic 25.0%
! AACACCGTAAATAATCAAAA+AGG - chr8_1:77846337-77846356 None:intergenic 25.0%
!! AAAAGTACCTTCAATGAGAA+GGG - chr8_1:77843440-77843459 None:intergenic 30.0%
!! AAAAAGTACCTTCAATGAGA+AGG - chr8_1:77843441-77843460 None:intergenic 30.0%
!!! GTACTGTGTTTTAGTTTAGT+TGG + chr8_1:77843949-77843968 Msa1216200:intron 30.0%
AATCAATTGATAAAGGGCAA+AGG - chr8_1:77844061-77844080 None:intergenic 30.0%
GGACACAAATCAATTGATAA+AGG - chr8_1:77844068-77844087 None:intergenic 30.0%
!!! ACTGCAAATCCTGTTTTAAT+AGG + chr8_1:77844297-77844316 Msa1216200:intron 30.0%
TATCAGCTCTTCAAATTTGT+TGG + chr8_1:77844332-77844351 Msa1216200:intron 30.0%
! ATCAGCTCTTCAAATTTGTT+GGG + chr8_1:77844333-77844352 Msa1216200:intron 30.0%
ATTAAATCCGCAAGCAAAAA+CGG + chr8_1:77844475-77844494 Msa1216200:intron 30.0%
GCAAATAAACGCTTAAAACT+AGG - chr8_1:77844679-77844698 None:intergenic 30.0%
! TTCCAGTAGTTTTCGTATTA+CGG + chr8_1:77844822-77844841 Msa1216200:intron 30.0%
GCAGCAATGAACTAATTATA+CGG - chr8_1:77845000-77845019 None:intergenic 30.0%
ATGTAATTAAGCTGCAACAA+TGG - chr8_1:77845056-77845075 None:intergenic 30.0%
! TGAAGGGTAGTATATGTTTT+TGG + chr8_1:77845162-77845181 Msa1216200:intron 30.0%
ATATGTAATGAGGACACATT+TGG + chr8_1:77845272-77845291 Msa1216200:intron 30.0%
CTTATGGTTTAAGTGACTTA+TGG + chr8_1:77845431-77845450 Msa1216200:intron 30.0%
! GAATGATTTTGTACTATGAG+TGG + chr8_1:77845677-77845696 Msa1216200:intron 30.0%
!!! ATGCATGGTTTTAGTTTCTA+AGG + chr8_1:77846198-77846217 Msa1216200:three_prime_UTR 30.0%
!!! TGCATGGTTTTAGTTTCTAA+GGG + chr8_1:77846199-77846218 Msa1216200:three_prime_UTR 30.0%
TAAAACAAAAGCTTAACAGC+TGG - chr8_1:77846226-77846245 None:intergenic 30.0%
ATCCTTGCATCATAATTGAT+TGG + chr8_1:77846265-77846284 Msa1216200:three_prime_UTR 30.0%
TCCTTGCATCATAATTGATT+GGG + chr8_1:77846266-77846285 Msa1216200:three_prime_UTR 30.0%
AAGAGTTTATTCCTTGTAGT+AGG + chr8_1:77846465-77846484 Msa1216200:three_prime_UTR 30.0%
GCACTCTTAGTAAATCTAAA+AGG + chr8_1:77846503-77846522 Msa1216200:three_prime_UTR 30.0%
AAATAAGCTTCCATTACGGA+GGG - chr8_1:77843395-77843414 None:intergenic 35.0%
! AAAGTACCTTCAATGAGAAG+GGG - chr8_1:77843439-77843458 None:intergenic 35.0%
AGAGATTGTGTTTCTCAGTT+GGG - chr8_1:77843596-77843615 None:intergenic 35.0%
AAGACAAGCTTCAAAACAGA+CGG - chr8_1:77843700-77843719 None:intergenic 35.0%
GTAACACAGATCTTGAAGAA+GGG - chr8_1:77843806-77843825 None:intergenic 35.0%
AGTAACACAGATCTTGAAGA+AGG - chr8_1:77843807-77843826 None:intergenic 35.0%
AACTGGATTCATCCTCATAA+AGG - chr8_1:77844032-77844051 None:intergenic 35.0%
!!! ATTTTTTCACTTTGTCTCGC+TGG - chr8_1:77844089-77844108 None:intergenic 35.0%
CAGAAGAAATGATCCGTAAT+TGG + chr8_1:77844145-77844164 Msa1216200:intron 35.0%
GAAATGATCCGTAATTGGAT+AGG + chr8_1:77844150-77844169 Msa1216200:intron 35.0%
TTGGATAGGAAAAAAGACTC+TGG + chr8_1:77844164-77844183 Msa1216200:intron 35.0%
!! TCAGCTCTTCAAATTTGTTG+GGG + chr8_1:77844334-77844353 Msa1216200:intron 35.0%
TTAAATCCGCAAGCAAAAAC+GGG + chr8_1:77844476-77844495 Msa1216200:intron 35.0%
CATAATAGCATCAAACGGTT+TGG - chr8_1:77844736-77844755 None:intergenic 35.0%
GCATGTAGCTATCTAAACTA+AGG - chr8_1:77844789-77844808 None:intergenic 35.0%
AATAGCAGCGTGAATCAAAA+TGG - chr8_1:77844916-77844935 None:intergenic 35.0%
GCTTAATTACATCAATCCGT+TGG + chr8_1:77845064-77845083 Msa1216200:intron 35.0%
TTCTAGATAGTTTCAGCCTA+AGG + chr8_1:77845135-77845154 Msa1216200:intron 35.0%
TCTAGATAGTTTCAGCCTAA+GGG + chr8_1:77845136-77845155 Msa1216200:intron 35.0%
ACATGCAGTAGGGAAAAAAT+AGG + chr8_1:77845340-77845359 Msa1216200:intron 35.0%
!! CAATATGTAGTTGCCACTTT+AGG + chr8_1:77845363-77845382 Msa1216200:intron 35.0%
ATACTGTCAATTGCCTAAAG+TGG - chr8_1:77845379-77845398 None:intergenic 35.0%
! ATTTTGTGTTAGGCCAACTA+TGG + chr8_1:77845403-77845422 Msa1216200:intron 35.0%
ACCATAAGAAATGCCATAGT+TGG - chr8_1:77845419-77845438 None:intergenic 35.0%
CTCAACAACCAAAACACTTA+TGG - chr8_1:77845556-77845575 None:intergenic 35.0%
!! GTAGTTTAGCAGTTTTGTAC+AGG + chr8_1:77845593-77845612 Msa1216200:intron 35.0%
! TTTTGTACTATGAGTGGTGT+TGG + chr8_1:77845683-77845702 Msa1216200:intron 35.0%
TTTGTACTATGAGTGGTGTT+GGG + chr8_1:77845684-77845703 Msa1216200:intron 35.0%
!!! CTGTTTTCTGCTGCTTTTTT+GGG + chr8_1:77845822-77845841 Msa1216200:CDS 35.0%
GTAGGTTATGGCATAGTTAT+GGG + chr8_1:77845933-77845952 Msa1216200:exon 35.0%
TGAAATCCAAAGATGACCTA+CGG - chr8_1:77845967-77845986 None:intergenic 35.0%
! AGTATCGAACCATCATTATG+GGG - chr8_1:77846052-77846071 None:intergenic 35.0%
! CAGTATCGAACCATCATTAT+GGG - chr8_1:77846053-77846072 None:intergenic 35.0%
! ACAGTATCGAACCATCATTA+TGG - chr8_1:77846054-77846073 None:intergenic 35.0%
!! ATTAGCTCTGCTAAAAAACC+AGG - chr8_1:77846106-77846125 None:intergenic 35.0%
ACCCAATCAATTATGATGCA+AGG - chr8_1:77846270-77846289 None:intergenic 35.0%
!! AAAAATCCGGTGCCATAAAA+AGG - chr8_1:77846311-77846330 None:intergenic 35.0%
!! AAAACACGCCCTATTTCTAT+AGG - chr8_1:77846363-77846382 None:intergenic 35.0%
GGAAAATTGATCCTACTACA+AGG - chr8_1:77846479-77846498 None:intergenic 35.0%
! AGATTTACTAAGAGTGCATC+AGG - chr8_1:77846500-77846519 None:intergenic 35.0%
AATAAGCTTCCATTACGGAG+GGG - chr8_1:77843394-77843413 None:intergenic 40.0%
GAAATAAGCTTCCATTACGG+AGG - chr8_1:77843396-77843415 None:intergenic 40.0%
GGGGAAATAAGCTTCCATTA+CGG - chr8_1:77843399-77843418 None:intergenic 40.0%
! AGATGAAGTGTGGACTGAAA+GGG - chr8_1:77843477-77843496 None:intergenic 40.0%
! AAGATGAAGTGTGGACTGAA+AGG - chr8_1:77843478-77843497 None:intergenic 40.0%
ATGGTAAGGAAGATGAAGTG+TGG - chr8_1:77843487-77843506 None:intergenic 40.0%
CAGAGATTGTGTTTCTCAGT+TGG - chr8_1:77843597-77843616 None:intergenic 40.0%
ATGATCTGTTTCACATGAGG+TGG - chr8_1:77843649-77843668 None:intergenic 40.0%
TGGATGATCTGTTTCACATG+AGG - chr8_1:77843652-77843671 None:intergenic 40.0%
GTGAAACAGATCATCCACTT+TGG + chr8_1:77843655-77843674 Msa1216200:CDS 40.0%
!!! GTCTGTTTTGAAGCTTGTCT+TGG + chr8_1:77843699-77843718 Msa1216200:CDS 40.0%
AAAGCTGTTCGAAATTCGTC+TGG - chr8_1:77843775-77843794 None:intergenic 40.0%
! CGAATTTCGAACAGCTTTGA+AGG + chr8_1:77843777-77843796 Msa1216200:intron 40.0%
ATTTCGACTACACATGGTCT+CGG - chr8_1:77844112-77844131 None:intergenic 40.0%
CCATCTTCTAATGCTGACAA+AGG - chr8_1:77844202-77844221 None:intergenic 40.0%
CCTTTGTCAGCATTAGAAGA+TGG + chr8_1:77844199-77844218 Msa1216200:intron 40.0%
! AGCTAGTGTTAGCATTGAAG+GGG - chr8_1:77844364-77844383 None:intergenic 40.0%
! GAGCTAGTGTTAGCATTGAA+GGG - chr8_1:77844365-77844384 None:intergenic 40.0%
!! TACATACCCGTTTTTGCTTG+CGG - chr8_1:77844485-77844504 None:intergenic 40.0%
!! TTCATGTTGTTAGCCGCTTT+TGG + chr8_1:77844598-77844617 Msa1216200:intron 40.0%
CCAAAACTAGATGCCAAAAG+CGG - chr8_1:77844614-77844633 None:intergenic 40.0%
TGCCGTAATACGAAAACTAC+TGG - chr8_1:77844827-77844846 None:intergenic 40.0%
GCACGTAAAATTGCTTCCAA+CGG - chr8_1:77845083-77845102 None:intergenic 40.0%
CTAGATAGTTTCAGCCTAAG+GGG + chr8_1:77845137-77845156 Msa1216200:intron 40.0%
TCAGTGGTAGAAATGCGTAT+AGG - chr8_1:77845208-77845227 None:intergenic 40.0%
TTTGGCATGAAACATGCAGT+AGG + chr8_1:77845329-77845348 Msa1216200:intron 40.0%
TTGGCATGAAACATGCAGTA+GGG + chr8_1:77845330-77845349 Msa1216200:intron 40.0%
GCCAACTATGGCATTTCTTA+TGG + chr8_1:77845415-77845434 Msa1216200:intron 40.0%
CCAAAACACTTATGGCATCT+TGG - chr8_1:77845548-77845567 None:intergenic 40.0%
! CCAAGATGCCATAAGTGTTT+TGG + chr8_1:77845545-77845564 Msa1216200:intron 40.0%
GTTTGATGCGGCAAAGTTAA+TGG + chr8_1:77845719-77845738 Msa1216200:intron 40.0%
TTTGGACAGTTTCGACGTTT+CGG + chr8_1:77845754-77845773 Msa1216200:intron 40.0%
CCAAAAAAGCAGCAGAAAAC+AGG - chr8_1:77845824-77845843 None:intergenic 40.0%
!!! CCTGTTTTCTGCTGCTTTTT+TGG + chr8_1:77845821-77845840 Msa1216200:CDS 40.0%
!!! CTGCTGGTTTTGATCTGTTA+TGG + chr8_1:77845864-77845883 Msa1216200:intron 40.0%
CGTAGGTTATGGCATAGTTA+TGG + chr8_1:77845932-77845951 Msa1216200:exon 40.0%
TGAATTCCGTAGGTCATCTT+TGG + chr8_1:77845958-77845977 Msa1216200:three_prime_UTR 40.0%
TGGATTTCAGTGTCATATCC+AGG + chr8_1:77845978-77845997 Msa1216200:three_prime_UTR 40.0%
GGATTTCAGTGTCATATCCA+GGG + chr8_1:77845979-77845998 Msa1216200:three_prime_UTR 40.0%
GATTTCAGTGTCATATCCAG+GGG + chr8_1:77845980-77845999 Msa1216200:three_prime_UTR 40.0%
! TCATTATGGGGAAAAGCATG+TGG - chr8_1:77846040-77846059 None:intergenic 40.0%
! CATGCTTTTCCCCATAATGA+TGG + chr8_1:77846040-77846059 Msa1216200:three_prime_UTR 40.0%
GATGGTTCGATACTGTTACA+AGG + chr8_1:77846058-77846077 Msa1216200:three_prime_UTR 40.0%
! TTACAAGGCTGTTGCTACTT+TGG + chr8_1:77846073-77846092 Msa1216200:three_prime_UTR 40.0%
GAGCTAATATGTTGTGTGTG+TGG + chr8_1:77846118-77846137 Msa1216200:three_prime_UTR 40.0%
!!! GGTGTTTTTAGGAATTGCGT+TGG + chr8_1:77846151-77846170 Msa1216200:three_prime_UTR 40.0%
!! TGCGTTGGTGCATTTTTGTT+AGG + chr8_1:77846166-77846185 Msa1216200:three_prime_UTR 40.0%
! GTGCATTTTTGTTAGGCAGA+GGG + chr8_1:77846173-77846192 Msa1216200:three_prime_UTR 40.0%
! TGCATTTTTGTTAGGCAGAG+GGG + chr8_1:77846174-77846193 Msa1216200:three_prime_UTR 40.0%
! GTACTGCCAAAGCCTTTTTA+TGG + chr8_1:77846296-77846315 Msa1216200:three_prime_UTR 40.0%
GTGTTCCGCCTATAGAAATA+GGG + chr8_1:77846352-77846371 Msa1216200:three_prime_UTR 40.0%
TGCCAAACCCTCCATTCTAT+TGG + chr8_1:77843359-77843378 Msa1216200:CDS 45.0%
CTCCAATAGAATGGAGGGTT+TGG - chr8_1:77843364-77843383 None:intergenic 45.0%
GGGTTCTCCAATAGAATGGA+GGG - chr8_1:77843369-77843388 None:intergenic 45.0%
TGGGTTCTCCAATAGAATGG+AGG - chr8_1:77843370-77843389 None:intergenic 45.0%
AGCTTATTTCCCCTCCGTAA+TGG + chr8_1:77843406-77843425 Msa1216200:CDS 45.0%
GAAGGGGAGAACTTCCATTA+CGG - chr8_1:77843423-77843442 None:intergenic 45.0%
AGTTCTCCCCTTCTCATTGA+AGG + chr8_1:77843430-77843449 Msa1216200:CDS 45.0%
! ATGAAGTGTGGACTGAAAGG+GGG - chr8_1:77843475-77843494 None:intergenic 45.0%
! GATGAAGTGTGGACTGAAAG+GGG - chr8_1:77843476-77843495 None:intergenic 45.0%
GTGATTGTGGTGACATGGTA+AGG - chr8_1:77843501-77843520 None:intergenic 45.0%
ATCTGTTTCACATGAGGTGG+CGG - chr8_1:77843646-77843665 None:intergenic 45.0%
!! CTGAGAATCGATGGTGGATA+AGG - chr8_1:77843727-77843746 None:intergenic 45.0%
!! CATCGATTCTCAGCCTTGTA+CGG + chr8_1:77843734-77843753 Msa1216200:CDS 45.0%
AGAAGCAACCATGGAGGTTT+CGG - chr8_1:77843841-77843860 None:intergenic 45.0%
AAGCTATCGAGAAGCAACCA+TGG - chr8_1:77843850-77843869 None:intergenic 45.0%
TTGTGTGATACCTACGATCC+AGG - chr8_1:77843999-77844018 None:intergenic 45.0%
TGGATCGTAGGTATCACACA+AGG + chr8_1:77843998-77844017 Msa1216200:intron 45.0%
AGGTATCACACAAGGTTCGT+TGG + chr8_1:77844006-77844025 Msa1216200:intron 45.0%
!! AAGGTTCGTTGGCCTTTATG+AGG + chr8_1:77844017-77844036 Msa1216200:intron 45.0%
AAGGGCAAAGGTAGTGAAAC+TGG - chr8_1:77844049-77844068 None:intergenic 45.0%
ACGCACATTTCGACTACACA+TGG - chr8_1:77844118-77844137 None:intergenic 45.0%
TAGTCGAAATGTGCGTGATG+AGG + chr8_1:77844122-77844141 Msa1216200:intron 45.0%
! GGAGCTAGTGTTAGCATTGA+AGG - chr8_1:77844366-77844385 None:intergenic 45.0%
!! CCGCTTTTGGCATCTAGTTT+TGG + chr8_1:77844611-77844630 Msa1216200:intron 45.0%
TTTCAGCCTAAGGGGTTTGA+AGG + chr8_1:77845145-77845164 Msa1216200:intron 45.0%
TTCAGCCTAAGGGGTTTGAA+GGG + chr8_1:77845146-77845165 Msa1216200:intron 45.0%
TACTACCCTTCAAACCCCTT+AGG - chr8_1:77845154-77845173 None:intergenic 45.0%
TAATGGTGTCCGCTTGCATT+TGG + chr8_1:77845736-77845755 Msa1216200:intron 45.0%
CGAAACTGTCCAAATGCAAG+CGG - chr8_1:77845748-77845767 None:intergenic 45.0%
TTGGACAGTTTCGACGTTTC+GGG + chr8_1:77845755-77845774 Msa1216200:intron 45.0%
AAAACCAGCAGCAGCAAAAC+AGG - chr8_1:77845855-77845874 None:intergenic 45.0%
AAAACTGCAGCACCACAATC+CGG - chr8_1:77845912-77845931 None:intergenic 45.0%
! GCTGCAGTTTTCGTAGGTTA+TGG + chr8_1:77845921-77845940 Msa1216200:exon 45.0%
GTTATGGGTCTGAATTCCGT+AGG + chr8_1:77845948-77845967 Msa1216200:three_prime_UTR 45.0%
AGATGTATGTGGGAGCTATG+TGG + chr8_1:77846004-77846023 Msa1216200:three_prime_UTR 45.0%
GATGTATGTGGGAGCTATGT+GGG + chr8_1:77846005-77846024 Msa1216200:three_prime_UTR 45.0%
!! GCAGCTCTGTTGGTGTTTTT+AGG + chr8_1:77846140-77846159 Msa1216200:three_prime_UTR 45.0%
! GGTGCATTTTTGTTAGGCAG+AGG + chr8_1:77846172-77846191 Msa1216200:three_prime_UTR 45.0%
! CCAAAGCCTTTTTATGGCAC+CGG + chr8_1:77846302-77846321 Msa1216200:three_prime_UTR 45.0%
GGTGTTCCGCCTATAGAAAT+AGG + chr8_1:77846351-77846370 Msa1216200:three_prime_UTR 45.0%
!! ACACGCCCTATTTCTATAGG+CGG - chr8_1:77846360-77846379 None:intergenic 45.0%
GGCTGGGTTCTCCAATAGAA+TGG - chr8_1:77843373-77843392 None:intergenic 50.0%
GGAGAACTTCCATTACGGAG+GGG - chr8_1:77843418-77843437 None:intergenic 50.0%
GGGAGAACTTCCATTACGGA+GGG - chr8_1:77843419-77843438 None:intergenic 50.0%
GAGGTGTGATTGTGGTGACA+TGG - chr8_1:77843506-77843525 None:intergenic 50.0%
! GTTTTGTGGATCTGGATCGG+TGG - chr8_1:77843535-77843554 None:intergenic 50.0%
!! GGGGTTTTGTGGATCTGGAT+CGG - chr8_1:77843538-77843557 None:intergenic 50.0%
!! CTGAAGGGGTTTTGTGGATC+TGG - chr8_1:77843543-77843562 None:intergenic 50.0%
AGAGAGATCCTGCTGCTGAA+GGG - chr8_1:77843558-77843577 None:intergenic 50.0%
GGATCTCTCTCGTTTGCTCT+TGG + chr8_1:77843568-77843587 Msa1216200:CDS 50.0%
GATCATCCACTTTGGTCCAC+CGG + chr8_1:77843663-77843682 Msa1216200:CDS 50.0%
AGCTTCAAAACAGACGGCGA+TGG - chr8_1:77843694-77843713 None:intergenic 50.0%
ACAAGGCTGAGAATCGATGG+TGG - chr8_1:77843733-77843752 None:intergenic 50.0%
CGTACAAGGCTGAGAATCGA+TGG - chr8_1:77843736-77843755 None:intergenic 50.0%
AATTCGTCTGGAGGCTTACG+CGG - chr8_1:77843763-77843782 None:intergenic 50.0%
GCTGTTCGAAATTCGTCTGG+AGG - chr8_1:77843772-77843791 None:intergenic 50.0%
CTATCGAGAAGCAACCATGG+AGG - chr8_1:77843847-77843866 None:intergenic 50.0%
! GCTTTTGCGTAAACGAAGCC+TGG + chr8_1:77843978-77843997 Msa1216200:intron 50.0%
GTAAACGAAGCCTGGATCGT+AGG + chr8_1:77843986-77844005 Msa1216200:intron 50.0%
TTTGCCGCATCAAACAGTCC+CGG - chr8_1:77845714-77845733 None:intergenic 50.0%
TTTCGACGTTTCGGGCTGTA+GGG + chr8_1:77845763-77845782 Msa1216200:CDS 50.0%
! GTTACCTGTTTTGCTGCTGC+TGG + chr8_1:77845848-77845867 Msa1216200:intron 50.0%
TGATCTGTTATGGCCGTTGC+AGG + chr8_1:77845874-77845893 Msa1216200:intron 50.0%
!!! TGTGGTGCTGCAGTTTTCGT+AGG + chr8_1:77845915-77845934 Msa1216200:CDS 50.0%
TATCCAGGGGCAGATGTATG+TGG + chr8_1:77845993-77846012 Msa1216200:three_prime_UTR 50.0%
ATCCAGGGGCAGATGTATGT+GGG + chr8_1:77845994-77846013 Msa1216200:three_prime_UTR 50.0%
!! CAAGGCTGTTGCTACTTTGG+TGG + chr8_1:77846076-77846095 Msa1216200:three_prime_UTR 50.0%
!! TGCTACTTTGGTGGAGTTCC+TGG + chr8_1:77846085-77846104 Msa1216200:three_prime_UTR 50.0%
!! CCGGTGCCATAAAAAGGCTT+TGG - chr8_1:77846305-77846324 None:intergenic 50.0%
GGGGAGAACTTCCATTACGG+AGG - chr8_1:77843420-77843439 None:intergenic 55.0%
! GGCAGCAAGAGGTGTGATTG+TGG - chr8_1:77843514-77843533 None:intergenic 55.0%
! CTGCTGCTGAAGGGGTTTTG+TGG - chr8_1:77843549-77843568 None:intergenic 55.0%
CACAAAACCCCTTCAGCAGC+AGG + chr8_1:77843547-77843566 Msa1216200:CDS 55.0%
GAGAGATCCTGCTGCTGAAG+GGG - chr8_1:77843557-77843576 None:intergenic 55.0%
GAGAGAGATCCTGCTGCTGA+AGG - chr8_1:77843559-77843578 None:intergenic 55.0%
AACCATGGAGGTTTCGGCTG+CGG - chr8_1:77843835-77843854 None:intergenic 55.0%
TTGCCGCATCAAACAGTCCC+GGG - chr8_1:77845713-77845732 None:intergenic 55.0%
GTTTCGACGTTTCGGGCTGT+AGG + chr8_1:77845762-77845781 Msa1216200:intron 55.0%
TTCGACGTTTCGGGCTGTAG+GGG + chr8_1:77845764-77845783 Msa1216200:CDS 55.0%
TGTGTGTGTGGCAGCTCTGT+TGG + chr8_1:77846130-77846149 Msa1216200:three_prime_UTR 55.0%
! GTTAGGCAGAGGGGTATGCA+TGG + chr8_1:77846183-77846202 Msa1216200:three_prime_UTR 55.0%
AGAACCCAGCCCCTCCGTAA+TGG + chr8_1:77843382-77843401 Msa1216200:CDS 60.0%
GCTTCCATTACGGAGGGGCT+GGG - chr8_1:77843389-77843408 None:intergenic 60.0%
AGCTTCCATTACGGAGGGGC+TGG - chr8_1:77843390-77843409 None:intergenic 60.0%
TCTGGATCGGTGGCAGCAAG+AGG - chr8_1:77843525-77843544 None:intergenic 60.0%
! TTCACATGAGGTGGCGGCTC+TGG - chr8_1:77843640-77843659 None:intergenic 60.0%
GCAGATCCGGTGGACCAAAG+TGG - chr8_1:77843672-77843691 None:intergenic 60.0%
ACAGACGGCGATGGCAGATC+CGG - chr8_1:77843685-77843704 None:intergenic 60.0%
! ATGAGTGGTGTTGGGCTCCC+CGG + chr8_1:77845692-77845711 Msa1216200:intron 60.0%
CTCCCCGGGACTGTTTGATG+CGG + chr8_1:77845707-77845726 Msa1216200:intron 60.0%
TGCCGCATCAAACAGTCCCG+GGG - chr8_1:77845712-77845731 None:intergenic 60.0%
TCGACGTTTCGGGCTGTAGG+GGG + chr8_1:77845765-77845784 Msa1216200:CDS 60.0%
TTATGGCCGTTGCAGGTGCC+TGG + chr8_1:77845881-77845900 Msa1216200:intron 60.0%
TATGGCCGTTGCAGGTGCCT+GGG + chr8_1:77845882-77845901 Msa1216200:intron 60.0%
GAAACCCCAGGCACCTGCAA+CGG - chr8_1:77845890-77845909 None:intergenic 60.0%
TTGCAGGTGCCTGGGGTTTC+CGG + chr8_1:77845890-77845909 Msa1216200:intron 60.0%
TGCCTGGGGTTTCCGGATTG+TGG + chr8_1:77845897-77845916 Msa1216200:CDS 60.0%
CACCACAATCCGGAAACCCC+AGG - chr8_1:77845902-77845921 None:intergenic 60.0%
CTCCCACATACATCTGCCCC+TGG - chr8_1:77845999-77846018 None:intergenic 60.0%
! ATGAGGTGGCGGCTCTGGTG+GGG - chr8_1:77843635-77843654 None:intergenic 65.0%
! CATGAGGTGGCGGCTCTGGT+GGG - chr8_1:77843636-77843655 None:intergenic 65.0%
! ACATGAGGTGGCGGCTCTGG+TGG - chr8_1:77843637-77843656 None:intergenic 65.0%
GCTTACGCGGAGGCCGTACA+AGG - chr8_1:77843750-77843769 None:intergenic 65.0%
TCGTCTGGAGGCTTACGCGG+AGG - chr8_1:77843760-77843779 None:intergenic 65.0%
GTCCGCAGCCGAAACCTCCA+TGG + chr8_1:77843830-77843849 Msa1216200:intron 65.0%
! TGAGTGGTGTTGGGCTCCCC+GGG + chr8_1:77845693-77845712 Msa1216200:intron 65.0%
! TTTCGGGCTGTAGGGGGCAC+TGG + chr8_1:77845771-77845790 Msa1216200:CDS 65.0%
ATGGCCGTTGCAGGTGCCTG+GGG + chr8_1:77845883-77845902 Msa1216200:intron 65.0%
! TGAGGTGGCGGCTCTGGTGG+GGG - chr8_1:77843634-77843653 None:intergenic 70.0%
GACGGCGATGGCAGATCCGG+TGG - chr8_1:77843682-77843701 None:intergenic 70.0%
! CGGCTCTGGTGGGGGACGCT+CGG - chr8_1:77843626-77843645 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 77843358 77846573 77843358 ID=Msa1216200;Name=Msa1216200
chr8_1 mRNA 77843358 77846573 77843358 ID=Msa1216200-mRNA-1;Parent=Msa1216200;Name=Msa1216200-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|0|0.33|0.66|0.5|0.33|3|637|175
chr8_1 exon 77843358 77843763 77843358 ID=Msa1216200-mRNA-1:exon:26896;Parent=Msa1216200-mRNA-1
chr8_1 exon 77845763 77845843 77845763 ID=Msa1216200-mRNA-1:exon:26897;Parent=Msa1216200-mRNA-1
chr8_1 exon 77845896 77846573 77845896 ID=Msa1216200-mRNA-1:exon:26898;Parent=Msa1216200-mRNA-1
chr8_1 CDS 77843358 77843763 77843358 ID=Msa1216200-mRNA-1:cds;Parent=Msa1216200-mRNA-1
chr8_1 CDS 77845763 77845843 77845763 ID=Msa1216200-mRNA-1:cds;Parent=Msa1216200-mRNA-1
chr8_1 CDS 77845896 77845936 77845896 ID=Msa1216200-mRNA-1:cds;Parent=Msa1216200-mRNA-1
chr8_1 three_prime_UTR 77845937 77846573 77845937 ID=Msa1216200-mRNA-1:three_prime_utr;Parent=Msa1216200-mRNA-1
Gene Sequence

>Msa1216200

ATGCCAAACCCTCCATTCTATTGGAGAACCCAGCCCCTCCGTAATGGAAGCTTATTTCCCCTCCGTAATGGAAGTTCTCCCCTTCTCATTGAAGGTACTTTTTGTTACTTGTTGCCCCCTTTCAGTCCACACTTCATCTTCCTTACCATGTCACCACAATCACACCTCTTGCTGCCACCGATCCAGATCCACAAAACCCCTTCAGCAGCAGGATCTCTCTCGTTTGCTCTTGGTACCCAACTGAGAAACACAATCTCTGTCTCAACCGAGCGTCCCCCACCAGAGCCGCCACCTCATGTGAAACAGATCATCCACTTTGGTCCACCGGATCTGCCATCGCCGTCTGTTTTGAAGCTTGTCTTGGCACCTTATCCACCATCGATTCTCAGCCTTGTACGGCCTCCGCTTTCGACGTTTCGGGCTGTAGGGGGCACTGGCGCTGCTGCTGTCATGACAGTGCAGCACCTGTTTTCTGCTGCTTTTTTGGGTGCCTGGGGTTTCCGGATTGTGGTGCTGCAGTTTTCGTAG
Protein sequence

>Msa1216200

MPNPPFYWRTQPLRNGSLFPLRNGSSPLLIEGTFCYLLPPFSPHFIFLTMSPQSHLLLPPIQIHKTPSAAGSLSFALGTQLRNTISVSTERPPPEPPPHVKQIIHFGPPDLPSPSVLKLVLAPYPPSILSLVRPPLSTFRAVGGTGAAAVMTVQHLFSAAFLGAWGFRIVVLQFS*