Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0824390 | XP_013444289.1 | 75.735 | 136 | 4 | 1 | 1 | 107 | 420 | 555 | 1.97e-58 | 205 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0824390 | sp|Q8LGU1|AB8C_ARATH | 57.353 | 136 | 29 | 1 | 1 | 107 | 598 | 733 | 2.17e-43 | 154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0824390 | A0A072TM89 | 75.735 | 136 | 4 | 1 | 1 | 107 | 420 | 555 | 9.41e-59 | 205 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0009420 | Msa0824390 | 0.801904 | 7.224742e-49 | -8.615850e-47 |
| Msa0064970 | Msa0824390 | 0.805524 | 1.280304e-49 | -8.615850e-47 |
| Msa0649110 | Msa0824390 | 0.800491 | 1.405661e-48 | -8.615850e-47 |
| Msa0663240 | Msa0824390 | 0.803241 | 3.829215e-49 | -8.615850e-47 |
| Msa0669410 | Msa0824390 | 0.822729 | 2.027169e-53 | -8.615850e-47 |
| Msa0766630 | Msa0824390 | 0.803211 | 3.884735e-49 | -8.615850e-47 |
| Msa0800210 | Msa0824390 | 0.818853 | 1.578202e-52 | -8.615850e-47 |
| Msa0824390 | Msa0842710 | 0.820975 | 5.161569e-53 | -8.615850e-47 |
| Msa0824390 | Msa0877250 | 0.800732 | 1.255509e-48 | -8.615850e-47 |
| Msa0824390 | Msa0899430 | 0.815650 | 8.292426e-52 | -8.615850e-47 |
| Msa0824390 | Msa0899450 | 0.829495 | 4.985655e-55 | -8.615850e-47 |
| Msa0824390 | Msa0925250 | 0.809016 | 2.329946e-50 | -8.615850e-47 |
| Msa0824390 | Msa0925290 | 0.842092 | 3.194258e-58 | -8.615850e-47 |
| Msa0824390 | Msa0927380 | 0.823404 | 1.410828e-53 | -8.615850e-47 |
| Msa0824390 | Msa0958550 | 0.808915 | 2.448768e-50 | -8.615850e-47 |
| Msa0824390 | Msa0964900 | 0.808774 | 2.624913e-50 | -8.615850e-47 |
| Msa0824390 | Msa0967280 | 0.841584 | 4.350148e-58 | -8.615850e-47 |
| Msa0824390 | Msa0976670 | 0.822628 | 2.139748e-53 | -8.615850e-47 |
| Msa0824390 | Msa0998680 | 0.818424 | 1.974256e-52 | -8.615850e-47 |
| Msa0824390 | Msa1149370 | 0.803821 | 2.902601e-49 | -8.615850e-47 |
| Msa0824390 | Msa1216200 | -0.802955 | 4.388721e-49 | -8.615850e-47 |
| Msa0824390 | Msa1248300 | 0.808947 | 2.410637e-50 | -8.615850e-47 |
| Msa0824390 | Msa1248310 | 0.807065 | 6.062964e-50 | -8.615850e-47 |
| Msa0824390 | Msa1248370 | 0.802219 | 6.222893e-49 | -8.615850e-47 |
| Msa0824390 | Msa1284680 | 0.802218 | 6.227880e-49 | -8.615850e-47 |
| Msa0824390 | Msa1403480 | 0.804544 | 2.053401e-49 | -8.615850e-47 |
| Msa0194440 | Msa0824390 | 0.806537 | 7.837770e-50 | -8.615850e-47 |
| Msa0384890 | Msa0824390 | 0.806107 | 9.659791e-50 | -8.615850e-47 |
| Msa0394660 | Msa0824390 | 0.804831 | 1.788581e-49 | -8.615850e-47 |
| Msa0434000 | Msa0824390 | 0.827431 | 1.570205e-54 | -8.615850e-47 |
| Msa0459260 | Msa0824390 | 0.812834 | 3.472548e-51 | -8.615850e-47 |
| Msa0479340 | Msa0824390 | 0.809788 | 1.591320e-50 | -8.615850e-47 |
| Msa0342750 | Msa0824390 | 0.805779 | 1.131852e-49 | -8.615850e-47 |
| Msa0493000 | Msa0824390 | 0.807275 | 5.471959e-50 | -8.615850e-47 |
| Msa0538600 | Msa0824390 | 0.807656 | 4.543909e-50 | -8.615850e-47 |
| Msa0598790 | Msa0824390 | 0.805083 | 1.584009e-49 | -8.615850e-47 |
| Msa0598880 | Msa0824390 | -0.802999 | 4.296651e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0824390 | MtrunA17_Chr8g0341751 | 75.735 | 136 | 4 | 1 | 1 | 107 | 424 | 559 | 1.96e-62 | 205 |
| Msa0824390 | MtrunA17_Chr8g0349441 | 74.242 | 132 | 9 | 1 | 1 | 107 | 45 | 176 | 5.91e-62 | 188 |
| Msa0824390 | MtrunA17_Chr8g0341761 | 68.382 | 136 | 14 | 1 | 1 | 107 | 596 | 731 | 2.86e-55 | 185 |
| Msa0824390 | MtrunA17_Chr8g0353781 | 66.176 | 136 | 17 | 1 | 1 | 107 | 599 | 734 | 9.25e-55 | 183 |
| Msa0824390 | MtrunA17_Chr6g0484361 | 66.176 | 136 | 17 | 1 | 1 | 107 | 604 | 739 | 5.26e-53 | 177 |
| Msa0824390 | MtrunA17_Chr8g0341811 | 65.672 | 134 | 17 | 1 | 3 | 107 | 439 | 572 | 5.90e-52 | 175 |
| Msa0824390 | MtrunA17_Chr8g0353541 | 64.706 | 136 | 19 | 1 | 1 | 107 | 208 | 343 | 2.26e-51 | 174 |
| Msa0824390 | MtrunA17_Chr8g0341851 | 55.882 | 136 | 31 | 1 | 1 | 107 | 606 | 741 | 8.52e-45 | 155 |
| Msa0824390 | MtrunA17_Chr7g0262191 | 45.588 | 136 | 45 | 1 | 1 | 107 | 639 | 774 | 6.47e-33 | 121 |
| Msa0824390 | MtrunA17_Chr5g0412721 | 44.853 | 136 | 46 | 1 | 1 | 107 | 619 | 754 | 9.80e-33 | 120 |
| Msa0824390 | MtrunA17_Chr5g0445741 | 44.853 | 136 | 46 | 1 | 1 | 107 | 630 | 765 | 1.82e-32 | 120 |
| Msa0824390 | MtrunA17_Chr5g0445671 | 44.853 | 136 | 46 | 1 | 1 | 107 | 239 | 374 | 2.11e-32 | 119 |
| Msa0824390 | MtrunA17_Chr6g0463201 | 44.030 | 134 | 46 | 1 | 3 | 107 | 627 | 760 | 2.39e-32 | 119 |
| Msa0824390 | MtrunA17_Chr8g0337121 | 47.015 | 134 | 42 | 2 | 3 | 107 | 629 | 762 | 3.20e-32 | 119 |
| Msa0824390 | MtrunA17_Chr1g0183191 | 46.324 | 136 | 44 | 1 | 1 | 107 | 643 | 778 | 3.24e-32 | 119 |
| Msa0824390 | MtrunA17_Chr6g0463141 | 43.284 | 134 | 47 | 1 | 3 | 107 | 621 | 754 | 3.89e-32 | 119 |
| Msa0824390 | MtrunA17_Chr6g0463401 | 42.647 | 136 | 49 | 1 | 1 | 107 | 628 | 763 | 6.86e-32 | 118 |
| Msa0824390 | MtrunA17_Chr6g0471281 | 42.647 | 136 | 49 | 1 | 1 | 107 | 621 | 756 | 8.04e-32 | 118 |
| Msa0824390 | MtrunA17_Chr8g0337191 | 46.269 | 134 | 43 | 2 | 3 | 107 | 609 | 742 | 1.02e-31 | 117 |
| Msa0824390 | MtrunA17_Chr3g0101481 | 41.912 | 136 | 50 | 1 | 1 | 107 | 624 | 759 | 2.78e-31 | 116 |
| Msa0824390 | MtrunA17_Chr3g0101461 | 42.647 | 136 | 49 | 1 | 1 | 107 | 432 | 567 | 7.80e-31 | 115 |
| Msa0824390 | MtrunA17_Chr3g0101441 | 43.382 | 136 | 48 | 1 | 1 | 107 | 432 | 567 | 8.43e-31 | 115 |
| Msa0824390 | MtrunA17_Chr3g0101471 | 42.647 | 136 | 49 | 1 | 1 | 107 | 430 | 565 | 1.29e-30 | 114 |
| Msa0824390 | MtrunA17_Chr5g0412921 | 44.118 | 136 | 47 | 1 | 1 | 107 | 610 | 745 | 1.35e-30 | 114 |
| Msa0824390 | MtrunA17_Chr6g0463131 | 41.176 | 136 | 51 | 1 | 1 | 107 | 621 | 756 | 2.72e-30 | 114 |
| Msa0824390 | MtrunA17_Chr6g0463211 | 41.791 | 134 | 49 | 1 | 3 | 107 | 660 | 793 | 4.73e-30 | 113 |
| Msa0824390 | MtrunA17_Chr6g0463181 | 41.045 | 134 | 50 | 1 | 3 | 107 | 609 | 742 | 5.20e-30 | 113 |
| Msa0824390 | MtrunA17_Chr6g0463121 | 40.441 | 136 | 52 | 1 | 1 | 107 | 620 | 755 | 6.19e-30 | 112 |
| Msa0824390 | MtrunA17_Chr6g0463151 | 38.806 | 134 | 53 | 1 | 3 | 107 | 440 | 573 | 2.19e-29 | 111 |
| Msa0824390 | MtrunA17_Chr1g0202221 | 42.336 | 137 | 49 | 2 | 1 | 107 | 618 | 754 | 3.40e-29 | 110 |
| Msa0824390 | MtrunA17_Chr8g0341781 | 87.500 | 56 | 7 | 0 | 1 | 56 | 435 | 490 | 2.27e-28 | 107 |
| Msa0824390 | MtrunA17_Chr6g0472531 | 39.706 | 136 | 53 | 1 | 1 | 107 | 571 | 706 | 3.02e-28 | 107 |
| Msa0824390 | MtrunA17_Chr1g0195691 | 38.235 | 136 | 55 | 1 | 1 | 107 | 650 | 785 | 1.40e-26 | 103 |
| Msa0824390 | MtrunA17_Chr8g0341791 | 88.000 | 50 | 6 | 0 | 58 | 107 | 37 | 86 | 3.79e-25 | 97.4 |
| Msa0824390 | MtrunA17_Chr8g0376961 | 41.844 | 141 | 48 | 4 | 1 | 107 | 583 | 723 | 6.37e-25 | 98.6 |
| Msa0824390 | MtrunA17_Chr2g0295911 | 40.441 | 136 | 52 | 1 | 1 | 107 | 641 | 776 | 7.10e-25 | 98.2 |
| Msa0824390 | MtrunA17_Chr2g0295901 | 40.441 | 136 | 52 | 2 | 1 | 107 | 641 | 776 | 1.19e-24 | 97.8 |
| Msa0824390 | MtrunA17_Chr8g0363771 | 41.176 | 136 | 51 | 2 | 1 | 107 | 636 | 771 | 2.06e-24 | 97.1 |
| Msa0824390 | MtrunA17_Chr2g0295921 | 37.500 | 136 | 56 | 1 | 1 | 107 | 653 | 788 | 1.19e-23 | 94.7 |
| Msa0824390 | MtrunA17_Chr3g0080941 | 36.986 | 146 | 61 | 2 | 1 | 115 | 615 | 760 | 5.13e-23 | 93.2 |
| Msa0824390 | MtrunA17_Chr3g0080981 | 37.671 | 146 | 60 | 2 | 1 | 115 | 615 | 760 | 5.86e-23 | 92.8 |
| Msa0824390 | MtrunA17_Chr2g0285731 | 35.862 | 145 | 63 | 1 | 1 | 115 | 615 | 759 | 1.46e-22 | 91.7 |
| Msa0824390 | MtrunA17_Chr3g0085281 | 42.478 | 113 | 34 | 2 | 26 | 107 | 617 | 729 | 1.40e-20 | 86.3 |
| Msa0824390 | MtrunA17_Chr2g0333911 | 37.226 | 137 | 54 | 4 | 3 | 107 | 159 | 295 | 5.20e-19 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0824390 | AT3G21250.4 | 57.353 | 136 | 29 | 1 | 1 | 107 | 604 | 739 | 2.10e-44 | 154 |
| Msa0824390 | AT3G21250.2 | 57.353 | 136 | 29 | 1 | 1 | 107 | 598 | 733 | 2.21e-44 | 154 |
| Msa0824390 | AT3G21250.1 | 57.353 | 136 | 29 | 1 | 1 | 107 | 598 | 733 | 2.34e-44 | 154 |
| Msa0824390 | AT3G21250.5 | 57.353 | 136 | 29 | 1 | 1 | 107 | 604 | 739 | 2.43e-44 | 154 |
| Msa0824390 | AT3G21250.6 | 57.353 | 136 | 29 | 1 | 1 | 107 | 182 | 317 | 3.07e-44 | 154 |
| Msa0824390 | AT3G21250.3 | 57.353 | 136 | 29 | 1 | 1 | 107 | 182 | 317 | 3.07e-44 | 154 |
| Msa0824390 | AT1G04120.2 | 45.588 | 136 | 45 | 1 | 1 | 107 | 622 | 757 | 1.08e-32 | 121 |
| Msa0824390 | AT1G04120.1 | 45.588 | 136 | 45 | 1 | 1 | 107 | 622 | 757 | 1.21e-32 | 120 |
| Msa0824390 | AT1G04120.3 | 45.588 | 136 | 45 | 1 | 1 | 107 | 587 | 722 | 1.30e-32 | 120 |
| Msa0824390 | AT3G60970.1 | 44.853 | 136 | 46 | 1 | 1 | 107 | 214 | 349 | 8.37e-32 | 118 |
| Msa0824390 | AT3G59140.1 | 43.704 | 135 | 46 | 2 | 3 | 107 | 603 | 737 | 1.37e-28 | 109 |
| Msa0824390 | AT3G62700.1 | 39.706 | 136 | 53 | 1 | 1 | 107 | 639 | 774 | 1.84e-28 | 108 |
| Msa0824390 | AT2G47800.1 | 38.971 | 136 | 54 | 1 | 1 | 107 | 641 | 776 | 1.19e-27 | 106 |
| Msa0824390 | AT3G60160.3 | 46.324 | 136 | 44 | 1 | 1 | 107 | 594 | 729 | 2.97e-25 | 99.8 |
| Msa0824390 | AT3G60160.2 | 46.324 | 136 | 44 | 1 | 1 | 107 | 630 | 765 | 3.05e-25 | 99.8 |
| Msa0824390 | AT3G60160.1 | 46.324 | 136 | 44 | 1 | 1 | 107 | 630 | 765 | 3.31e-25 | 99.8 |
| Msa0824390 | AT2G34660.3 | 35.862 | 145 | 63 | 1 | 1 | 115 | 327 | 471 | 1.47e-23 | 95.1 |
| Msa0824390 | AT2G34660.1 | 35.862 | 145 | 63 | 1 | 1 | 115 | 614 | 758 | 1.75e-23 | 94.7 |
| Msa0824390 | AT2G34660.2 | 35.862 | 145 | 63 | 1 | 1 | 115 | 614 | 758 | 1.75e-23 | 94.7 |
| Msa0824390 | AT1G30400.2 | 35.862 | 145 | 63 | 1 | 1 | 115 | 614 | 758 | 8.52e-23 | 92.8 |
| Msa0824390 | AT1G30400.1 | 35.862 | 145 | 63 | 1 | 1 | 115 | 614 | 758 | 8.52e-23 | 92.8 |
| Msa0824390 | AT1G30410.5 | 35.862 | 145 | 63 | 2 | 1 | 115 | 500 | 644 | 1.14e-22 | 92.4 |
| Msa0824390 | AT1G30410.3 | 35.862 | 145 | 63 | 2 | 1 | 115 | 615 | 759 | 1.33e-22 | 92.4 |
| Msa0824390 | AT1G30410.2 | 35.862 | 145 | 63 | 2 | 1 | 115 | 615 | 759 | 1.38e-22 | 92.4 |
| Msa0824390 | AT1G30410.6 | 35.862 | 145 | 63 | 2 | 1 | 115 | 615 | 759 | 1.38e-22 | 92.4 |
| Msa0824390 | AT1G30410.4 | 35.862 | 145 | 63 | 2 | 1 | 115 | 615 | 759 | 1.38e-22 | 92.4 |
| Msa0824390 | AT1G30410.1 | 35.862 | 145 | 63 | 2 | 1 | 115 | 618 | 762 | 1.43e-22 | 92.0 |
| Msa0824390 | AT3G13080.3 | 41.176 | 136 | 51 | 1 | 1 | 107 | 640 | 775 | 2.24e-22 | 91.7 |
| Msa0824390 | AT3G13080.4 | 41.176 | 136 | 51 | 1 | 1 | 107 | 640 | 775 | 2.24e-22 | 91.7 |
| Msa0824390 | AT3G13080.2 | 41.176 | 136 | 51 | 1 | 1 | 107 | 640 | 775 | 2.54e-22 | 91.7 |
| Msa0824390 | AT3G13080.1 | 41.176 | 136 | 51 | 1 | 1 | 107 | 640 | 775 | 2.80e-22 | 91.3 |
| Msa0824390 | AT3G13080.5 | 41.176 | 136 | 51 | 1 | 1 | 107 | 640 | 775 | 3.08e-22 | 91.3 |
| Msa0824390 | AT1G30420.1 | 35.172 | 145 | 64 | 2 | 1 | 115 | 615 | 759 | 8.62e-22 | 90.1 |
| Msa0824390 | AT1G30420.2 | 35.172 | 145 | 64 | 2 | 1 | 115 | 615 | 759 | 1.18e-21 | 89.7 |
| Msa0824390 | AT3G13090.1 | 41.176 | 136 | 51 | 1 | 1 | 107 | 601 | 736 | 2.73e-21 | 88.6 |
| Msa0824390 | AT3G13100.1 | 41.176 | 136 | 51 | 1 | 1 | 107 | 624 | 759 | 2.03e-20 | 85.9 |
| Msa0824390 | AT3G13100.2 | 41.176 | 136 | 51 | 1 | 1 | 107 | 379 | 514 | 2.54e-20 | 85.9 |
| Msa0824390 | AT2G07680.2 | 32.847 | 137 | 60 | 4 | 3 | 107 | 582 | 718 | 1.06e-16 | 75.5 |
| Msa0824390 | AT2G07680.6 | 32.847 | 137 | 60 | 4 | 3 | 107 | 483 | 619 | 1.56e-16 | 75.1 |
| Msa0824390 | AT2G07680.4 | 32.847 | 137 | 60 | 4 | 3 | 107 | 557 | 693 | 1.64e-16 | 74.7 |
| Msa0824390 | AT2G07680.1 | 32.847 | 137 | 60 | 4 | 3 | 107 | 560 | 696 | 1.66e-16 | 74.7 |
| Msa0824390 | AT2G07680.5 | 32.847 | 137 | 60 | 4 | 3 | 107 | 566 | 702 | 1.72e-16 | 74.7 |
| Msa0824390 | AT2G07680.3 | 32.847 | 137 | 60 | 4 | 3 | 107 | 582 | 718 | 1.74e-16 | 74.7 |
| Msa0824390 | AT1G71330.1 | 61.702 | 47 | 18 | 0 | 61 | 107 | 1 | 47 | 1.79e-13 | 65.9 |
Find 30 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTACAGTTCCTGAAATCTT+TGG | 0.238475 | 5_3:+63623057 | None:intergenic |
| GGAGAGATTCCAAAGATTTC+AGG | 0.251036 | 5_3:-63623066 | Msa0824390:CDS |
| GAATTGTGCTCTTCTCCTTC+AGG | 0.295397 | 5_3:-63622633 | Msa0824390:CDS |
| CATGGTGATCTTACAGAAAT+TGG | 0.314895 | 5_3:-63622708 | Msa0824390:CDS |
| TTCAAGATGGAAACTTCATT+TGG | 0.320619 | 5_3:-63623209 | Msa0824390:CDS |
| ATCATTGATATCCTTATCTA+AGG | 0.340848 | 5_3:+63622737 | None:intergenic |
| ACCTCTCACTCTTCTCCTTC+AGG | 0.363797 | 5_3:-63622604 | Msa0824390:CDS |
| ATATTAACTTCTCTTAAAGT+TGG | 0.375416 | 5_3:+63623168 | None:intergenic |
| CTCATATCTTGCTTTGTCCA+TGG | 0.384238 | 5_3:+63622779 | None:intergenic |
| GAGAAGTTAATATAGAAATC+AGG | 0.454792 | 5_3:-63623158 | Msa0824390:CDS |
| GATATCCTTATCTAAGGCAC+AGG | 0.504393 | 5_3:+63622743 | None:intergenic |
| TAAAGCCTGTGCCTTAGATA+AGG | 0.519806 | 5_3:-63622748 | Msa0824390:CDS |
| GTGTGTGGACCAGTTGGAGC+TGG | 0.527728 | 5_3:-63623117 | Msa0824390:CDS |
| GATATCAATGATTTCAGCCA+TGG | 0.551981 | 5_3:-63622726 | Msa0824390:CDS |
| CAGAGAGGGATCAATATAAG+TGG | 0.558760 | 5_3:-63622684 | Msa0824390:CDS |
| AATGCTATGGAAATTCAAGA+TGG | 0.564810 | 5_3:-63623222 | None:intergenic |
| CAGAGAGTCAGTGAACCTGA+AGG | 0.579352 | 5_3:+63622589 | None:intergenic |
| AGTTAATATAGAAATCAGGC+GGG | 0.581386 | 5_3:-63623154 | Msa0824390:CDS |
| GGACAGAAAATTGCAGTGTG+TGG | 0.582870 | 5_3:-63623132 | Msa0824390:CDS |
| ATTGCAGTGTGTGGACCAGT+TGG | 0.590180 | 5_3:-63623123 | Msa0824390:CDS |
| ACCTGAAGGAGAAGAGTGAG+AGG | 0.599277 | 5_3:+63622603 | None:intergenic |
| TTACAGAAATTGGTCAGAGA+GGG | 0.601531 | 5_3:-63622698 | Msa0824390:CDS |
| TGAAGGAGAAGAGTGAGAGG+TGG | 0.607006 | 5_3:+63622606 | None:intergenic |
| TAAGTGGAGGACAAAAGCAA+AGG | 0.636679 | 5_3:-63622668 | Msa0824390:CDS |
| CTTACAGAAATTGGTCAGAG+AGG | 0.638609 | 5_3:-63622699 | Msa0824390:CDS |
| GTGAGAGGTGGAGAACCTGA+AGG | 0.640647 | 5_3:+63622618 | None:intergenic |
| AGAGGGATCAATATAAGTGG+AGG | 0.647017 | 5_3:-63622681 | Msa0824390:CDS |
| AAGTTAATATAGAAATCAGG+CGG | 0.651493 | 5_3:-63623155 | Msa0824390:CDS |
| GTTAATATAGAAATCAGGCG+GGG | 0.668485 | 5_3:-63623153 | Msa0824390:CDS |
| CAATTTCTGTAAGATCACCA+TGG | 0.706817 | 5_3:+63622709 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTCAGATACAAATAATA+GGG | + | chr5_3:63622901-63622920 | None:intergenic | 15.0% |
| !! | TAATTTCAGATACAAATAAT+AGG | + | chr5_3:63622902-63622921 | None:intergenic | 15.0% |
| !! | ATATTAACTTCTCTTAAAGT+TGG | + | chr5_3:63622647-63622666 | None:intergenic | 20.0% |
| ! | GAGAAGTTAATATAGAAATC+AGG | - | chr5_3:63622654-63622673 | Msa0824390:CDS | 25.0% |
| ! | AAGTTAATATAGAAATCAGG+CGG | - | chr5_3:63622657-63622676 | Msa0824390:CDS | 25.0% |
| ! | ATGAGTAAATATCCAAAAAG+AGG | - | chr5_3:63622799-63622818 | Msa0824390:intron | 25.0% |
| ! | TGAGTAAATATCCAAAAAGA+GGG | - | chr5_3:63622800-63622819 | Msa0824390:intron | 25.0% |
| !! | AAAGACATTTTGCAATTTCA+CGG | - | chr5_3:63622832-63622851 | Msa0824390:intron | 25.0% |
| ! | TATTAATAATGTGAGAGACT+AGG | - | chr5_3:63622866-63622885 | Msa0824390:intron | 25.0% |
| ! | ACATAGTATTAGTAGTATCT+TGG | - | chr5_3:63622967-63622986 | Msa0824390:intron | 25.0% |
| ! | GTTCAAGATAATACACTATT+TGG | - | chr5_3:63623005-63623024 | Msa0824390:intron | 25.0% |
| ! | ATCATTGATATCCTTATCTA+AGG | + | chr5_3:63623078-63623097 | None:intergenic | 25.0% |
| TTCAAGATGGAAACTTCATT+TGG | - | chr5_3:63622603-63622622 | Msa0824390:CDS | 30.0% | |
| AGTTAATATAGAAATCAGGC+GGG | - | chr5_3:63622658-63622677 | Msa0824390:CDS | 30.0% | |
| ! | TCTCTTTTGTATGCAATACT+AGG | - | chr5_3:63622725-63622744 | Msa0824390:CDS | 30.0% |
| GTTAATATAGAAATCAGGCG+GGG | - | chr5_3:63622659-63622678 | Msa0824390:CDS | 35.0% | |
| CTTACAGTTCCTGAAATCTT+TGG | + | chr5_3:63622758-63622777 | None:intergenic | 35.0% | |
| !! | TTCAAGTTTGTCCCTCTTTT+TGG | + | chr5_3:63622814-63622833 | None:intergenic | 35.0% |
| GTAGTATCTTGGATACAAAG+TGG | - | chr5_3:63622978-63622997 | Msa0824390:intron | 35.0% | |
| GATATCAATGATTTCAGCCA+TGG | - | chr5_3:63623086-63623105 | Msa0824390:CDS | 35.0% | |
| CAATTTCTGTAAGATCACCA+TGG | + | chr5_3:63623106-63623125 | None:intergenic | 35.0% | |
| CATGGTGATCTTACAGAAAT+TGG | - | chr5_3:63623104-63623123 | Msa0824390:CDS | 35.0% | |
| TTACAGAAATTGGTCAGAGA+GGG | - | chr5_3:63623114-63623133 | Msa0824390:CDS | 35.0% | |
| GGAGAGATTCCAAAGATTTC+AGG | - | chr5_3:63622746-63622765 | Msa0824390:CDS | 40.0% | |
| TACACTATTTGGCAAGACCA+TGG | - | chr5_3:63623016-63623035 | Msa0824390:intron | 40.0% | |
| CTCATATCTTGCTTTGTCCA+TGG | + | chr5_3:63623036-63623055 | None:intergenic | 40.0% | |
| TAAAGCCTGTGCCTTAGATA+AGG | - | chr5_3:63623064-63623083 | Msa0824390:CDS | 40.0% | |
| ! | GATATCCTTATCTAAGGCAC+AGG | + | chr5_3:63623072-63623091 | None:intergenic | 40.0% |
| CTTACAGAAATTGGTCAGAG+AGG | - | chr5_3:63623113-63623132 | Msa0824390:CDS | 40.0% | |
| CAGAGAGGGATCAATATAAG+TGG | - | chr5_3:63623128-63623147 | Msa0824390:CDS | 40.0% | |
| AGAGGGATCAATATAAGTGG+AGG | - | chr5_3:63623131-63623150 | Msa0824390:CDS | 40.0% | |
| TAAGTGGAGGACAAAAGCAA+AGG | - | chr5_3:63623144-63623163 | Msa0824390:CDS | 40.0% | |
| GGACAGAAAATTGCAGTGTG+TGG | - | chr5_3:63622680-63622699 | Msa0824390:CDS | 45.0% | |
| ! | GATGATTTTCCAGCTCCAAC+TGG | + | chr5_3:63622707-63622726 | None:intergenic | 45.0% |
| GAATTGTGCTCTTCTCCTTC+AGG | - | chr5_3:63623179-63623198 | Msa0824390:CDS | 45.0% | |
| ATTGCAGTGTGTGGACCAGT+TGG | - | chr5_3:63622689-63622708 | Msa0824390:CDS | 50.0% | |
| TGAAGGAGAAGAGTGAGAGG+TGG | + | chr5_3:63623209-63623228 | None:intergenic | 50.0% | |
| ACCTCTCACTCTTCTCCTTC+AGG | - | chr5_3:63623208-63623227 | Msa0824390:CDS | 50.0% | |
| ACCTGAAGGAGAAGAGTGAG+AGG | + | chr5_3:63623212-63623231 | None:intergenic | 50.0% | |
| GTGAGAGGTGGAGAACCTGA+AGG | + | chr5_3:63623197-63623216 | None:intergenic | 55.0% | |
| GTGTGTGGACCAGTTGGAGC+TGG | - | chr5_3:63622695-63622714 | Msa0824390:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 63622596 | 63623238 | 63622596 | ID=Msa0824390;Name=Msa0824390 |
| chr5_3 | mRNA | 63622596 | 63623238 | 63622596 | ID=Msa0824390-mRNA-1;Parent=Msa0824390;Name=Msa0824390-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0|0|1|1|1|2|0|127 |
| chr5_3 | exon | 63623062 | 63623238 | 63623062 | ID=Msa0824390-mRNA-1:exon:19879;Parent=Msa0824390-mRNA-1 |
| chr5_3 | exon | 63622596 | 63622802 | 63622596 | ID=Msa0824390-mRNA-1:exon:19878;Parent=Msa0824390-mRNA-1 |
| chr5_3 | CDS | 63623062 | 63623238 | 63623062 | ID=Msa0824390-mRNA-1:cds;Parent=Msa0824390-mRNA-1 |
| chr5_3 | CDS | 63622596 | 63622802 | 63622596 | ID=Msa0824390-mRNA-1:cds;Parent=Msa0824390-mRNA-1 |
| Gene Sequence |
| Protein sequence |
