Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1218440 | KEH32332.1 | 75.301 | 166 | 27 | 3 | 1 | 156 | 12 | 173 | 2.10e-52 | 175 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0382030 | Msa1218440 | 0.821633 | 3.639982e-53 | -8.615850e-47 |
| Msa0431530 | Msa1218440 | 0.818516 | 1.881680e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1218440 | MtrunA17_Chr4g0068021 | 75.301 | 166 | 27 | 3 | 1 | 156 | 12 | 173 | 1.93e-56 | 175 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 59 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTAGGTGGAGGAGGAGG+GGG | 0.295895 | 8_1:-81100034 | None:intergenic |
| TGGAGATGGAACTTGGTTGC+AGG | 0.305413 | 8_1:-81099963 | None:intergenic |
| GGAAGTAGGTGGAGGAGGAG+GGG | 0.312049 | 8_1:-81100035 | None:intergenic |
| GAGGAAGTAGGTGGAGGAGG+AGG | 0.320009 | 8_1:-81100037 | None:intergenic |
| GATGAAGGTGGTGGCGGTGA+TGG | 0.355172 | 8_1:-81099992 | None:intergenic |
| AAGAAGGATGATAGAACAAT+TGG | 0.357797 | 8_1:-81100322 | None:intergenic |
| AAAGGAAAATAAGGAACAAT+TGG | 0.359271 | 8_1:-81100238 | None:intergenic |
| TGGTAATAGAAAGGAAAATA+AGG | 0.366977 | 8_1:-81100247 | None:intergenic |
| GGGAGGACAGTTGCTGGTGG+TGG | 0.367624 | 8_1:-81100014 | None:intergenic |
| GGTGGCGGTGATGGTGGTGG+TGG | 0.387584 | 8_1:-81099983 | None:intergenic |
| TGTGCACTGGATCTGTGCTT+TGG | 0.399775 | 8_1:-81099918 | None:intergenic |
| GAAGGTGGTGGCGGTGATGG+TGG | 0.419882 | 8_1:-81099989 | None:intergenic |
| AAATAAGGAACAATTGGATT+AGG | 0.423566 | 8_1:-81100232 | None:intergenic |
| GTGGTGGTGGAGATGGAACT+TGG | 0.425859 | 8_1:-81099970 | None:intergenic |
| GGTGGTGGCGGTGATGGTGG+TGG | 0.429431 | 8_1:-81099986 | None:intergenic |
| CTGGATCTGTGCTTTGGAGG+TGG | 0.433353 | 8_1:-81099912 | None:intergenic |
| AGACGAAGTAGGTGCGGAGG+AGG | 0.447114 | 8_1:-81100284 | None:intergenic |
| GGTGCGGAGGAGGGTGGTGG+AGG | 0.451281 | 8_1:-81100274 | None:intergenic |
| GGAGATGGAACTTGGTTGCA+GGG | 0.458766 | 8_1:-81099962 | None:intergenic |
| CCTCCTCCACCTACTTCCTC+TGG | 0.467195 | 8_1:+81100040 | Msa1218440:CDS |
| GTAGGTGCGGAGGAGGGTGG+TGG | 0.468381 | 8_1:-81100277 | None:intergenic |
| GGATTAGGTGGTGGTGGAGT+TGG | 0.468533 | 8_1:-81100217 | None:intergenic |
| CCACCTACTTCCTCTGGTGG+TGG | 0.470519 | 8_1:+81100046 | Msa1218440:CDS |
| GCACTGGATCTGTGCTTTGG+AGG | 0.478366 | 8_1:-81099915 | None:intergenic |
| TCCTCTGGTGGTGGATCAAG+TGG | 0.478487 | 8_1:+81100055 | Msa1218440:CDS |
| AGTGGCGGAAGCAGCGGCGG+AGG | 0.478610 | 8_1:+81100151 | Msa1218440:intron |
| GTGGTGGATGAAGGTGGTGG+CGG | 0.484951 | 8_1:-81099998 | None:intergenic |
| ACAATTGGATTAGGTGGTGG+TGG | 0.492183 | 8_1:-81100223 | None:intergenic |
| GGTGATGGTGGTGGTGGAGA+TGG | 0.492880 | 8_1:-81099977 | None:intergenic |
| CAGTTGCTGGTGGTGGATGA+AGG | 0.499288 | 8_1:-81100007 | None:intergenic |
| AGGAAGTAGGTGGAGGAGGA+GGG | 0.503787 | 8_1:-81100036 | None:intergenic |
| CCAGAGGAAGTAGGTGGAGG+AGG | 0.511251 | 8_1:-81100040 | None:intergenic |
| TCCAGTGGCGGAAGCAGCGG+CGG | 0.523539 | 8_1:+81100148 | Msa1218440:intron |
| GGACCGTAGTATCTGTTACT+AGG | 0.524627 | 8_1:-81100196 | None:intergenic |
| TAGTATCTGTTACTAGGTGG+AGG | 0.529762 | 8_1:-81100190 | None:intergenic |
| CTGGTGGTGGATGAAGGTGG+TGG | 0.534938 | 8_1:-81100001 | None:intergenic |
| AAGAGTGAGCGAGACGAAGT+AGG | 0.537046 | 8_1:-81100295 | None:intergenic |
| TAAGGAACAATTGGATTAGG+TGG | 0.544270 | 8_1:-81100229 | None:intergenic |
| TGAAAGCAACAACATGAAGA+AGG | 0.546201 | 8_1:-81100338 | None:intergenic |
| TCATCCAGTGGCGGAAGCAG+CGG | 0.555726 | 8_1:+81100145 | Msa1218440:intron |
| GGAGGACTGTGGTAATAGAA+AGG | 0.563513 | 8_1:-81100256 | None:intergenic |
| CCACCAGAGGAAGTAGGTGG+AGG | 0.576757 | 8_1:-81100043 | None:intergenic |
| GGAACAATTGGATTAGGTGG+TGG | 0.577304 | 8_1:-81100226 | None:intergenic |
| GAAGTAGGTGCGGAGGAGGG+TGG | 0.577992 | 8_1:-81100280 | None:intergenic |
| GCGAGACGAAGTAGGTGCGG+AGG | 0.578517 | 8_1:-81100287 | None:intergenic |
| AGGAGGGTGGTGGAGGACTG+TGG | 0.581790 | 8_1:-81100267 | None:intergenic |
| AAGCAGCACACATTGTGCAC+TGG | 0.593488 | 8_1:-81099931 | None:intergenic |
| TTGCTGGTGGTGGATGAAGG+TGG | 0.599278 | 8_1:-81100004 | None:intergenic |
| CCTCCACCTACTTCCTCTGG+TGG | 0.613868 | 8_1:+81100043 | Msa1218440:CDS |
| GACGAAGTAGGTGCGGAGGA+GGG | 0.617607 | 8_1:-81100283 | None:intergenic |
| GAGATGGAACTTGGTTGCAG+GGG | 0.618756 | 8_1:-81099961 | None:intergenic |
| TCTGGTGGTGGATCAAGTGG+AGG | 0.623168 | 8_1:+81100058 | Msa1218440:CDS |
| TCCACTTGATCCACCACCAG+AGG | 0.623500 | 8_1:-81100056 | None:intergenic |
| CCACCTAGTAACAGATACTA+CGG | 0.626231 | 8_1:+81100193 | Msa1218440:CDS |
| GATCCACCACCAGAGGAAGT+AGG | 0.628044 | 8_1:-81100049 | None:intergenic |
| TGAGCGAGACGAAGTAGGTG+CGG | 0.630530 | 8_1:-81100290 | None:intergenic |
| AGTATCTGTTACTAGGTGGA+GGG | 0.632538 | 8_1:-81100189 | None:intergenic |
| CCACCACCAGAGGAAGTAGG+TGG | 0.649431 | 8_1:-81100046 | None:intergenic |
| CCGTAGTATCTGTTACTAGG+TGG | 0.663783 | 8_1:-81100193 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAGTGAAGCTAAAAA+AGG | + | chr8_1:81100367-81100386 | Msa1218440:CDS | 20.0% |
| ! | AAATAAGGAACAATTGGATT+AGG | - | chr8_1:81100235-81100254 | None:intergenic | 25.0% |
| ! | AAAGGAAAATAAGGAACAAT+TGG | - | chr8_1:81100241-81100260 | None:intergenic | 25.0% |
| ! | TGGTAATAGAAAGGAAAATA+AGG | - | chr8_1:81100250-81100269 | None:intergenic | 25.0% |
| AAGAAGGATGATAGAACAAT+TGG | - | chr8_1:81100325-81100344 | None:intergenic | 30.0% | |
| TAAGTGTAAGAATGAGATGG+TGG | - | chr8_1:81100106-81100125 | None:intergenic | 35.0% | |
| GAGTAAGTGTAAGAATGAGA+TGG | - | chr8_1:81100109-81100128 | None:intergenic | 35.0% | |
| ! | TAAGGAACAATTGGATTAGG+TGG | - | chr8_1:81100232-81100251 | None:intergenic | 35.0% |
| TGAAAGCAACAACATGAAGA+AGG | - | chr8_1:81100341-81100360 | None:intergenic | 35.0% | |
| !! | CTTTGGAGGTGGTTTTTATG+TGG | - | chr8_1:81099904-81099923 | None:intergenic | 40.0% |
| AGTATCTGTTACTAGGTGGA+GGG | - | chr8_1:81100192-81100211 | None:intergenic | 40.0% | |
| TAGTATCTGTTACTAGGTGG+AGG | - | chr8_1:81100193-81100212 | None:intergenic | 40.0% | |
| CCACCTAGTAACAGATACTA+CGG | + | chr8_1:81100193-81100212 | Msa1218440:CDS | 40.0% | |
| !! | TAAGAATGAGATGGTGGAGG+TGG | - | chr8_1:81100100-81100119 | None:intergenic | 45.0% |
| ! | GTGTAAGAATGAGATGGTGG+AGG | - | chr8_1:81100103-81100122 | None:intergenic | 45.0% |
| CCGTAGTATCTGTTACTAGG+TGG | - | chr8_1:81100196-81100215 | None:intergenic | 45.0% | |
| GGACCGTAGTATCTGTTACT+AGG | - | chr8_1:81100199-81100218 | None:intergenic | 45.0% | |
| ! | ACAATTGGATTAGGTGGTGG+TGG | - | chr8_1:81100226-81100245 | None:intergenic | 45.0% |
| ! | GGAACAATTGGATTAGGTGG+TGG | - | chr8_1:81100229-81100248 | None:intergenic | 45.0% |
| GGAGGACTGTGGTAATAGAA+AGG | - | chr8_1:81100259-81100278 | None:intergenic | 45.0% | |
| TGTGCACTGGATCTGTGCTT+TGG | - | chr8_1:81099921-81099940 | None:intergenic | 50.0% | |
| AAGCAGCACACATTGTGCAC+TGG | - | chr8_1:81099934-81099953 | None:intergenic | 50.0% | |
| GAGATGGAACTTGGTTGCAG+GGG | - | chr8_1:81099964-81099983 | None:intergenic | 50.0% | |
| GGAGATGGAACTTGGTTGCA+GGG | - | chr8_1:81099965-81099984 | None:intergenic | 50.0% | |
| TGGAGATGGAACTTGGTTGC+AGG | - | chr8_1:81099966-81099985 | None:intergenic | 50.0% | |
| AAGAGTGAGCGAGACGAAGT+AGG | - | chr8_1:81100298-81100317 | None:intergenic | 50.0% | |
| !! | CTGGATCTGTGCTTTGGAGG+TGG | - | chr8_1:81099915-81099934 | None:intergenic | 55.0% |
| GCACTGGATCTGTGCTTTGG+AGG | - | chr8_1:81099918-81099937 | None:intergenic | 55.0% | |
| !! | GTGGTGGTGGAGATGGAACT+TGG | - | chr8_1:81099973-81099992 | None:intergenic | 55.0% |
| !! | TTGCTGGTGGTGGATGAAGG+TGG | - | chr8_1:81100007-81100026 | None:intergenic | 55.0% |
| !! | CAGTTGCTGGTGGTGGATGA+AGG | - | chr8_1:81100010-81100029 | None:intergenic | 55.0% |
| AGGAAGTAGGTGGAGGAGGA+GGG | - | chr8_1:81100039-81100058 | None:intergenic | 55.0% | |
| GATCCACCACCAGAGGAAGT+AGG | - | chr8_1:81100052-81100071 | None:intergenic | 55.0% | |
| !! | TCCTCTGGTGGTGGATCAAG+TGG | + | chr8_1:81100055-81100074 | Msa1218440:CDS | 55.0% |
| TCCACTTGATCCACCACCAG+AGG | - | chr8_1:81100059-81100078 | None:intergenic | 55.0% | |
| !! | TCTGGTGGTGGATCAAGTGG+AGG | + | chr8_1:81100058-81100077 | Msa1218440:CDS | 55.0% |
| !! | GGATTAGGTGGTGGTGGAGT+TGG | - | chr8_1:81100220-81100239 | None:intergenic | 55.0% |
| TGAGCGAGACGAAGTAGGTG+CGG | - | chr8_1:81100293-81100312 | None:intergenic | 55.0% | |
| !! | GGTGATGGTGGTGGTGGAGA+TGG | - | chr8_1:81099980-81099999 | None:intergenic | 60.0% |
| !! | GATGAAGGTGGTGGCGGTGA+TGG | - | chr8_1:81099995-81100014 | None:intergenic | 60.0% |
| !! | GTGGTGGATGAAGGTGGTGG+CGG | - | chr8_1:81100001-81100020 | None:intergenic | 60.0% |
| !! | CTGGTGGTGGATGAAGGTGG+TGG | - | chr8_1:81100004-81100023 | None:intergenic | 60.0% |
| GAAGTAGGTGGAGGAGGAGG+GGG | - | chr8_1:81100037-81100056 | None:intergenic | 60.0% | |
| GGAAGTAGGTGGAGGAGGAG+GGG | - | chr8_1:81100038-81100057 | None:intergenic | 60.0% | |
| GAGGAAGTAGGTGGAGGAGG+AGG | - | chr8_1:81100040-81100059 | None:intergenic | 60.0% | |
| CCAGAGGAAGTAGGTGGAGG+AGG | - | chr8_1:81100043-81100062 | None:intergenic | 60.0% | |
| CCTCCTCCACCTACTTCCTC+TGG | + | chr8_1:81100040-81100059 | Msa1218440:CDS | 60.0% | |
| CCACCAGAGGAAGTAGGTGG+AGG | - | chr8_1:81100046-81100065 | None:intergenic | 60.0% | |
| CCTCCACCTACTTCCTCTGG+TGG | + | chr8_1:81100043-81100062 | Msa1218440:CDS | 60.0% | |
| CCACCACCAGAGGAAGTAGG+TGG | - | chr8_1:81100049-81100068 | None:intergenic | 60.0% | |
| CCACCTACTTCCTCTGGTGG+TGG | + | chr8_1:81100046-81100065 | Msa1218440:CDS | 60.0% | |
| CTGCTTCCGCCACTGGATGA+CGG | - | chr8_1:81100145-81100164 | None:intergenic | 60.0% | |
| ! | TCATCCAGTGGCGGAAGCAG+CGG | + | chr8_1:81100145-81100164 | Msa1218440:intron | 60.0% |
| GACGAAGTAGGTGCGGAGGA+GGG | - | chr8_1:81100286-81100305 | None:intergenic | 60.0% | |
| AGACGAAGTAGGTGCGGAGG+AGG | - | chr8_1:81100287-81100306 | None:intergenic | 60.0% | |
| !! | GAAGGTGGTGGCGGTGATGG+TGG | - | chr8_1:81099992-81100011 | None:intergenic | 65.0% |
| GGGAGGACAGTTGCTGGTGG+TGG | - | chr8_1:81100017-81100036 | None:intergenic | 65.0% | |
| AGGGGGAGGACAGTTGCTGG+TGG | - | chr8_1:81100020-81100039 | None:intergenic | 65.0% | |
| AGGAGGGGGAGGACAGTTGC+TGG | - | chr8_1:81100023-81100042 | None:intergenic | 65.0% | |
| !! | GGATGACGGAGGAGGTGGAG+AGG | - | chr8_1:81100131-81100150 | None:intergenic | 65.0% |
| !! | CCACTGGATGACGGAGGAGG+TGG | - | chr8_1:81100136-81100155 | None:intergenic | 65.0% |
| CCACCTCCTCCGTCATCCAG+TGG | + | chr8_1:81100133-81100152 | Msa1218440:intron | 65.0% | |
| CCTCCTCCGTCATCCAGTGG+CGG | + | chr8_1:81100136-81100155 | Msa1218440:intron | 65.0% | |
| ! | CTTCCGCCACTGGATGACGG+AGG | - | chr8_1:81100142-81100161 | None:intergenic | 65.0% |
| !! | AGGAGGGTGGTGGAGGACTG+TGG | - | chr8_1:81100270-81100289 | None:intergenic | 65.0% |
| GAAGTAGGTGCGGAGGAGGG+TGG | - | chr8_1:81100283-81100302 | None:intergenic | 65.0% | |
| GCGAGACGAAGTAGGTGCGG+AGG | - | chr8_1:81100290-81100309 | None:intergenic | 65.0% | |
| !! | GGTGGCGGTGATGGTGGTGG+TGG | - | chr8_1:81099986-81100005 | None:intergenic | 70.0% |
| !! | GGTGGTGGCGGTGATGGTGG+TGG | - | chr8_1:81099989-81100008 | None:intergenic | 70.0% |
| GTAGGTGGAGGAGGAGGGGG+AGG | - | chr8_1:81100034-81100053 | None:intergenic | 70.0% | |
| !! | CCGCCACTGGATGACGGAGG+AGG | - | chr8_1:81100139-81100158 | None:intergenic | 70.0% |
| ! | TCCAGTGGCGGAAGCAGCGG+CGG | + | chr8_1:81100148-81100167 | Msa1218440:intron | 70.0% |
| GTAGGTGCGGAGGAGGGTGG+TGG | - | chr8_1:81100280-81100299 | None:intergenic | 70.0% | |
| TCCGCCGCTGCTTCCGCCAC+TGG | - | chr8_1:81100152-81100171 | None:intergenic | 75.0% | |
| ! | AGTGGCGGAAGCAGCGGCGG+AGG | + | chr8_1:81100151-81100170 | Msa1218440:intron | 75.0% |
| ! | GGTGCGGAGGAGGGTGGTGG+AGG | - | chr8_1:81100277-81100296 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 81099848 | 81100397 | 81099848 | ID=Msa1218440;Name=Msa1218440 |
| chr8_1 | mRNA | 81099848 | 81100397 | 81099848 | ID=Msa1218440-mRNA-1;Parent=Msa1218440;Name=Msa1218440-mRNA-1;_AED=0.08;_eAED=0.17;_QI=0|0|0|1|0|0|2|0|159 |
| chr8_1 | exon | 81099848 | 81100079 | 81099848 | ID=Msa1218440-mRNA-1:exon:28663;Parent=Msa1218440-mRNA-1 |
| chr8_1 | exon | 81100153 | 81100397 | 81100153 | ID=Msa1218440-mRNA-1:exon:28664;Parent=Msa1218440-mRNA-1 |
| chr8_1 | CDS | 81099848 | 81100079 | 81099848 | ID=Msa1218440-mRNA-1:cds;Parent=Msa1218440-mRNA-1 |
| chr8_1 | CDS | 81100153 | 81100397 | 81100153 | ID=Msa1218440-mRNA-1:cds;Parent=Msa1218440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
