AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0431530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0431530 MtrunA17_Chr3g0136301 96.507 229 6 1 1 227 1 229 4.17e-165 454
Msa0431530 MtrunA17_Chr8g0336911 45.026 191 103 2 32 221 30 219 2.28e-54 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0431530 AT1G02180.1 44.000 175 92 6 32 203 29 200 3.77e-43 145

Find 78 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAGGGTCTCCTTATTTC+TGG 0.118257 3_2:+83577959 Msa0431530:CDS
TTCATCCTTTCCTTCCTTCT+TGG 0.129768 3_2:+83575619 Msa0431530:CDS
AAGTACCTTCTTAAAGATTC+TGG 0.222866 3_2:-83575413 None:intergenic
TCGTATAACTGGTCGTTTCT+TGG 0.250063 3_2:+83577810 Msa0431530:CDS
GCTTCAGCAAATTGAGATTC+AGG 0.261524 3_2:-83577748 None:intergenic
AGCTCTTCTCGTTTCTTATC+AGG 0.274033 3_2:-83577879 None:intergenic
CGTATAACTGGTCGTTTCTT+GGG 0.276791 3_2:+83577811 Msa0431530:CDS
GTACCCTATAGCCCTGAAAA+AGG 0.292646 3_2:+83578430 Msa0431530:three_prime_UTR
ATGCCTTAACACCACAGTTT+GGG 0.323486 3_2:-83577777 None:intergenic
ATTCAAGATGCATTTGGTTA+TGG 0.323548 3_2:+83578404 Msa0431530:three_prime_UTR
AACAACACACACCAGAAATA+AGG 0.333485 3_2:-83577970 None:intergenic
ACCAAACGTGTGAGTGAGTT+GGG 0.348682 3_2:-83575493 None:intergenic
GATGCCTTAACACCACAGTT+TGG 0.357391 3_2:-83577778 None:intergenic
TCAACTCTTGCTCGTATAAC+TGG 0.364017 3_2:+83577799 Msa0431530:CDS
TGCCCCAAACTGTGGTGTTA+AGG 0.376413 3_2:+83577774 Msa0431530:CDS
TCAAAAGCAAATGTGCTGTT+AGG 0.377113 3_2:-83578006 None:intergenic
GCATATGAAACATGTGATAT+TGG 0.378061 3_2:+83578323 Msa0431530:three_prime_UTR
GCTTCAGTTTCAATTGCAAT+GGG 0.382192 3_2:+83578135 Msa0431530:CDS
ATTGGAATTCAAGATGCATT+TGG 0.388458 3_2:+83578398 Msa0431530:three_prime_UTR
CAAGTTGGTGCTGCTGTAAC+CGG 0.391700 3_2:+83577931 Msa0431530:CDS
TTCCTTCTTGGTGCTTCTAC+CGG 0.398930 3_2:+83575631 Msa0431530:CDS
ACAAAACCAGGTTGCTATAG+TGG 0.403030 3_2:+83578048 Msa0431530:CDS
TGCTTCAGTTTCAATTGCAA+TGG 0.413253 3_2:+83578134 Msa0431530:CDS
TGCTTTGATTGTTATACGAT+TGG 0.413715 3_2:+83578380 Msa0431530:three_prime_UTR
TCTCGCCCTACAGTACATTA+AGG 0.413855 3_2:+83577675 Msa0431530:CDS
CCCCAACTCACTCACACGTT+TGG 0.438855 3_2:+83575492 Msa0431530:five_prime_UTR
TCATCTTTATTTGACAATCC+CGG 0.454811 3_2:+83577643 Msa0431530:CDS
TGTGCAGTGGTGCTCATGTT+TGG 0.457962 3_2:+83578082 Msa0431530:CDS
CAACTCACTCACACGTTTGG+TGG 0.462241 3_2:+83575495 Msa0431530:five_prime_UTR
ATATCACATGTTTCATATGC+AGG 0.471229 3_2:-83578320 None:intergenic
TAGGGCGAGACATGCGAGCC+CGG 0.474850 3_2:-83577662 None:intergenic
GCAGCTACTAACTTGTCTGC+AGG 0.476484 3_2:-83577589 None:intergenic
CATCTTTATTTGACAATCCC+GGG 0.500468 3_2:+83577644 Msa0431530:CDS
GGTGATTGTGGTGCTGTGGG+TGG 0.502206 3_2:+83577706 Msa0431530:CDS
GGTATGCCTTAATGTACTGT+AGG 0.503759 3_2:-83577681 None:intergenic
CAGGGTGATTGTGGTGCTGT+GGG 0.510763 3_2:+83577703 Msa0431530:CDS
CTTCAGCAAATTGAGATTCA+GGG 0.511447 3_2:-83577747 None:intergenic
CGAAAACAGAACTGCTCACA+AGG 0.516931 3_2:+83577618 Msa0431530:CDS
GTGCATGGACATACTTAGTC+TGG 0.524594 3_2:-83577837 None:intergenic
GTATAACTGGTCGTTTCTTG+GGG 0.529253 3_2:+83577812 Msa0431530:CDS
TGGTGTGTGTTGTTTAGCAA+TGG 0.533016 3_2:+83577979 Msa0431530:CDS
CCAGGGTGATTGTGGTGCTG+TGG 0.539111 3_2:+83577702 Msa0431530:CDS
AATAGCCACATCACACTGAC+TGG 0.539928 3_2:-83578108 None:intergenic
TACTTCCAGAATCTTTAAGA+AGG 0.542813 3_2:+83575408 Msa0431530:five_prime_UTR
CACCAAACGTGTGAGTGAGT+TGG 0.545998 3_2:-83575494 None:intergenic
AAGGCATACCAGGGTGATTG+TGG 0.548506 3_2:+83577694 Msa0431530:CDS
ACAGTACATTAAGGCATACC+AGG 0.557681 3_2:+83577684 Msa0431530:CDS
CAGAAAACGCCTCAGGTGCA+TGG 0.559426 3_2:-83577852 None:intergenic
GCTGCTGTAACCGGTACTGA+TGG 0.569000 3_2:+83577940 Msa0431530:CDS
AGTAAGAATCACACTCAAGT+TGG 0.576187 3_2:+83577916 Msa0431530:CDS
AAGCACCAAGAAGGAAGGAA+AGG 0.578158 3_2:-83575624 None:intergenic
AGGGCGAGACATGCGAGCCC+GGG 0.579446 3_2:-83577661 None:intergenic
TGCCTTAACACCACAGTTTG+GGG 0.580489 3_2:-83577776 None:intergenic
CTTTGGTAGCATGTGAGTGG+TGG 0.580649 3_2:-83575439 None:intergenic
CACCGGTAGAAGCACCAAGA+AGG 0.580761 3_2:-83575633 None:intergenic
GCTGTAACCGGTACTGATGG+AGG 0.581331 3_2:+83577943 Msa0431530:CDS
AGGATTTCAGAAAACGCCTC+AGG 0.582162 3_2:-83577859 None:intergenic
CCTCTGTAGTGACAAAATGA+AGG 0.583962 3_2:-83578203 None:intergenic
GGTAGAAGCACCAAGAAGGA+AGG 0.585888 3_2:-83575629 None:intergenic
AGGAGACCCTCCATCAGTAC+CGG 0.597918 3_2:-83577950 None:intergenic
GTATGCCTTAATGTACTGTA+GGG 0.599616 3_2:-83577680 None:intergenic
TTTCTTTGGTAGCATGTGAG+TGG 0.604521 3_2:-83575442 None:intergenic
TTCAGCAAATTGAGATTCAG+GGG 0.606803 3_2:-83577746 None:intergenic
GGACTTACTTTGAGTAGCAC+CGG 0.613594 3_2:-83575650 None:intergenic
GGTCACCAGTCAGTGTGATG+TGG 0.613666 3_2:+83578103 Msa0431530:CDS
CAGCTACTAACTTGTCTGCA+GGG 0.614123 3_2:-83577588 None:intergenic
CTTCAGTTTCAATTGCAATG+GGG 0.618866 3_2:+83578136 Msa0431530:CDS
CTGTAACCGGTACTGATGGA+GGG 0.623210 3_2:+83577944 Msa0431530:CDS
AAATAACAAAATCAAAACAA+AGG 0.645136 3_2:-83578258 None:intergenic
TTAGCTCCACTATAGCAACC+TGG 0.653166 3_2:-83578054 None:intergenic
CCAAACGTGTGAGTGAGTTG+GGG 0.654948 3_2:-83575492 None:intergenic
TCAGCAAATTGAGATTCAGG+GGG 0.657704 3_2:-83577745 None:intergenic
CCACAGCACCACAATCACCC+TGG 0.672377 3_2:-83577702 None:intergenic
GTGGCTAAAGAAACAAAACC+AGG 0.685787 3_2:+83578036 Msa0431530:CDS
TAAGTATGTCCATGCACCTG+AGG 0.686474 3_2:+83577843 Msa0431530:CDS
GTGTCTGCTTCAATGTAGTG+AGG 0.687007 3_2:-83575523 None:intergenic
CAGTACATTAAGGCATACCA+GGG 0.710618 3_2:+83577685 Msa0431530:CDS
GGAGCTAATGATGTGTGCAG+TGG 0.715652 3_2:+83578069 Msa0431530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGTCATTAAAAAAAAAT+TGG + chr3_2:83576320-83576339 Msa0431530:intron 10.0%
!!! ATTTTCATTGATTATATATA+TGG + chr3_2:83577342-83577361 Msa0431530:intron 10.0%
!!! AATAAAAAAAAGACTTGTTT+TGG - chr3_2:83575674-83575693 None:intergenic 15.0%
!!! TTTATTTTATATCTCAGATT+TGG + chr3_2:83575688-83575707 Msa0431530:intron 15.0%
!! ATTAAAAAAAAATTGGTTGT+AGG + chr3_2:83576327-83576346 Msa0431530:intron 15.0%
!! AGAAAATAAATATAACTATG+AGG - chr3_2:83577458-83577477 None:intergenic 15.0%
!! AAATAACAAAATCAAAACAA+AGG - chr3_2:83578261-83578280 None:intergenic 15.0%
!!! TGTTTTTTTCTTTCTTTCTT+TGG - chr3_2:83575459-83575478 None:intergenic 20.0%
!! CTATAACTCATTAAAGTTTA+TGG + chr3_2:83576054-83576073 Msa0431530:intron 20.0%
!! GACAATATAAAATGAAATCA+AGG - chr3_2:83576422-83576441 None:intergenic 20.0%
!! AAAAAAATTGAACTGTTATG+TGG + chr3_2:83576603-83576622 Msa0431530:intron 20.0%
!! AAATATGTAACCTTAATCAA+AGG - chr3_2:83577318-83577337 None:intergenic 20.0%
!! ATAAATATAACTATGAGGTA+GGG - chr3_2:83577453-83577472 None:intergenic 20.0%
!! AATAAATATAACTATGAGGT+AGG - chr3_2:83577454-83577473 None:intergenic 20.0%
!!! TGAATTTTTACTTGATTTAG+TGG + chr3_2:83578180-83578199 Msa0431530:three_prime_UTR 20.0%
! TCTTGCAAGTTGTATTAAAA+TGG + chr3_2:83575898-83575917 Msa0431530:intron 25.0%
! TGAAAGTTCCAAATAAGATA+CGG - chr3_2:83575941-83575960 None:intergenic 25.0%
!! ATTTGGAACTTTCACTATAA+TGG + chr3_2:83575947-83575966 Msa0431530:intron 25.0%
! TATGTTAGTTAGTCACAAAT+TGG + chr3_2:83576172-83576191 Msa0431530:intron 25.0%
! TTAAACAATAAGCATCAAAC+AGG - chr3_2:83576501-83576520 None:intergenic 25.0%
! TTGTTTAAGAGCTAATTATG+TGG + chr3_2:83576513-83576532 Msa0431530:intron 25.0%
! TTTCAACAAAATTAATCCAG+GGG - chr3_2:83576542-83576561 None:intergenic 25.0%
! CTTTCAACAAAATTAATCCA+GGG - chr3_2:83576543-83576562 None:intergenic 25.0%
! TCTTTCAACAAAATTAATCC+AGG - chr3_2:83576544-83576563 None:intergenic 25.0%
!! ATGACTTTTGTAGTGAAATT+AGG + chr3_2:83576733-83576752 Msa0431530:intron 25.0%
! TGAAATTAGGACTAAACTTA+TGG + chr3_2:83576746-83576765 Msa0431530:intron 25.0%
!!! TGAATATCTAGCTTTCATTA+TGG + chr3_2:83576876-83576895 Msa0431530:intron 25.0%
! AGACAAACTATAACAAATGT+TGG - chr3_2:83577062-83577081 None:intergenic 25.0%
!!! ACATTTGTTATAGTTTGTCT+AGG + chr3_2:83577062-83577081 Msa0431530:intron 25.0%
! AACCTTAATCAAAGGATAAA+CGG - chr3_2:83577310-83577329 None:intergenic 25.0%
! GTAAAAATTCATGATCACAA+TGG - chr3_2:83578171-83578190 None:intergenic 25.0%
!!! TGATTTTGTTATTTGTTGAG+AGG + chr3_2:83578267-83578286 Msa0431530:three_prime_UTR 25.0%
!! AAATTGTTGCATAAGCTTTT+TGG + chr3_2:83578457-83578476 Msa0431530:three_prime_UTR 25.0%
! TACTTCCAGAATCTTTAAGA+AGG + chr3_2:83575408-83575427 Msa0431530:five_prime_UTR 30.0%
AAGTACCTTCTTAAAGATTC+TGG - chr3_2:83575416-83575435 None:intergenic 30.0%
TATGTAACATGAGTTTGATC+TGG + chr3_2:83575729-83575748 Msa0431530:intron 30.0%
ATTACTGTGAAATGCTACAT+TGG - chr3_2:83575817-83575836 None:intergenic 30.0%
AGGAAAAAGAGAACAAGTTA+AGG + chr3_2:83575986-83576005 Msa0431530:intron 30.0%
ATTGTCCAAATCAAGAATCA+CGG - chr3_2:83576017-83576036 None:intergenic 30.0%
!!! TTATGGTCTTTGTTTTAGTG+TGG + chr3_2:83576071-83576090 Msa0431530:intron 30.0%
TTGTGACTAACTAACATAAG+TGG - chr3_2:83576170-83576189 None:intergenic 30.0%
TTTATAACTGTATACGCTTC+CGG + chr3_2:83576376-83576395 Msa0431530:intron 30.0%
! CATTTTATATTGTCTCTGCT+TGG + chr3_2:83576428-83576447 Msa0431530:intron 30.0%
AATTGAACTGTTATGTGGTT+AGG + chr3_2:83576608-83576627 Msa0431530:intron 30.0%
ACAAAAGTCATAACTTTGCA+TGG - chr3_2:83576724-83576743 None:intergenic 30.0%
AAACTTATGGAAGCCTTAAA+TGG + chr3_2:83576759-83576778 Msa0431530:intron 30.0%
! GGATTTAGGAATTTGAGATT+TGG + chr3_2:83576818-83576837 Msa0431530:intron 30.0%
ACAGAGTTTAGTCTATACAA+AGG + chr3_2:83576937-83576956 Msa0431530:intron 30.0%
CAGAGTTTAGTCTATACAAA+GGG + chr3_2:83576938-83576957 Msa0431530:intron 30.0%
AAGATAAATAAAACCGTTGC+TGG + chr3_2:83576971-83576990 Msa0431530:intron 30.0%
ACAATGATAGAAGCAATATC+AGG - chr3_2:83577025-83577044 None:intergenic 30.0%
TAGTGACTGTATTTCAAACA+CGG + chr3_2:83577251-83577270 Msa0431530:intron 30.0%
TAATCAAAGGATAAACGGAA+AGG - chr3_2:83577305-83577324 None:intergenic 30.0%
TTCCGTTTATCCTTTGATTA+AGG + chr3_2:83577305-83577324 Msa0431530:intron 30.0%
AATTTAGAGAACACACAAGT+GGG - chr3_2:83577520-83577539 None:intergenic 30.0%
TAATTTAGAGAACACACAAG+TGG - chr3_2:83577521-83577540 None:intergenic 30.0%
! TCATCTTTATTTGACAATCC+CGG + chr3_2:83577643-83577662 Msa0431530:CDS 30.0%
ATATCACATGTTTCATATGC+AGG - chr3_2:83578323-83578342 None:intergenic 30.0%
GCATATGAAACATGTGATAT+TGG + chr3_2:83578323-83578342 Msa0431530:three_prime_UTR 30.0%
! CTCTTGTAATTTTCTCATCT+AGG + chr3_2:83578346-83578365 Msa0431530:three_prime_UTR 30.0%
TGCTTTGATTGTTATACGAT+TGG + chr3_2:83578380-83578399 Msa0431530:three_prime_UTR 30.0%
ATTGGAATTCAAGATGCATT+TGG + chr3_2:83578398-83578417 Msa0431530:three_prime_UTR 30.0%
ATTCAAGATGCATTTGGTTA+TGG + chr3_2:83578404-83578423 Msa0431530:three_prime_UTR 30.0%
! AATTCTGCTAGCTGTAGTTT+TGG + chr3_2:83575834-83575853 Msa0431530:intron 35.0%
AGAACTGTCCGTATCTTATT+TGG + chr3_2:83575930-83575949 Msa0431530:intron 35.0%
TGGAACTTTCACTATAATGG+TGG + chr3_2:83575950-83575969 Msa0431530:intron 35.0%
ATATCCCGTGATTCTTGATT+TGG + chr3_2:83576009-83576028 Msa0431530:intron 35.0%
TTGTCCAAATCAAGAATCAC+GGG - chr3_2:83576016-83576035 None:intergenic 35.0%
GAGTTATAGAGACGAAGAAA+TGG - chr3_2:83576043-83576062 None:intergenic 35.0%
!! GTTTTAGTGTGGAGTTTCAT+TGG + chr3_2:83576082-83576101 Msa0431530:intron 35.0%
GTTAGTCACAAATTGGTTGT+AGG + chr3_2:83576179-83576198 Msa0431530:intron 35.0%
!! AGTTCAACTGGTTTGAGTTA+AGG + chr3_2:83576224-83576243 Msa0431530:intron 35.0%
!! GTTCAACTGGTTTGAGTTAA+GGG + chr3_2:83576225-83576244 Msa0431530:intron 35.0%
TGAGTTAAGGGATAAAGAGT+TGG + chr3_2:83576237-83576256 Msa0431530:intron 35.0%
! ACGTGGACATAGTTCATTTT+TGG - chr3_2:83576456-83576475 None:intergenic 35.0%
CTAGAGCTAGAAACATAGAT+AGG - chr3_2:83576582-83576601 None:intergenic 35.0%
!! CTATCTATGTTTCTAGCTCT+AGG + chr3_2:83576580-83576599 Msa0431530:intron 35.0%
AACAGAAGTCTCATCTTCAA+AGG - chr3_2:83576674-83576693 None:intergenic 35.0%
AAAGTCATAACTTTGCATGG+TGG - chr3_2:83576721-83576740 None:intergenic 35.0%
TACAAAGAGGTCACCATTTA+AGG - chr3_2:83576775-83576794 None:intergenic 35.0%
TATCTGTGTCAAGTACAAAG+AGG - chr3_2:83576788-83576807 None:intergenic 35.0%
!! GATAGAGAGTACTAGGATTT+AGG + chr3_2:83576804-83576823 Msa0431530:intron 35.0%
TAAGAGTGGAGAAAATGTTG+TGG + chr3_2:83576848-83576867 Msa0431530:intron 35.0%
TATAGACTAAACTCTGTCTC+TGG - chr3_2:83576934-83576953 None:intergenic 35.0%
ATGATAGAAGCAATATCAGG+AGG - chr3_2:83577022-83577041 None:intergenic 35.0%
TGAGTTTCTCAACAGATTCT+CGG - chr3_2:83577128-83577147 None:intergenic 35.0%
AAAGTAGGCTGTTTATTGTG+AGG - chr3_2:83577375-83577394 None:intergenic 35.0%
! CACAATAAACAGCCTACTTT+TGG + chr3_2:83577375-83577394 Msa0431530:intron 35.0%
!! TTTTTCAGTGAGCCAAAAGT+AGG - chr3_2:83577390-83577409 None:intergenic 35.0%
!!! TACTTTTGGCTCACTGAAAA+AGG + chr3_2:83577389-83577408 Msa0431530:intron 35.0%
!!! ACTTTTGGCTCACTGAAAAA+GGG + chr3_2:83577390-83577409 Msa0431530:intron 35.0%
! CATCTTTATTTGACAATCCC+GGG + chr3_2:83577644-83577663 Msa0431530:CDS 35.0%
GTATGCCTTAATGTACTGTA+GGG - chr3_2:83577683-83577702 None:intergenic 35.0%
TTCAGCAAATTGAGATTCAG+GGG - chr3_2:83577749-83577768 None:intergenic 35.0%
CTTCAGCAAATTGAGATTCA+GGG - chr3_2:83577750-83577769 None:intergenic 35.0%
AGTAAGAATCACACTCAAGT+TGG + chr3_2:83577916-83577935 Msa0431530:CDS 35.0%
AACAACACACACCAGAAATA+AGG - chr3_2:83577973-83577992 None:intergenic 35.0%
! TCAAAAGCAAATGTGCTGTT+AGG - chr3_2:83578009-83578028 None:intergenic 35.0%
!! TAACAGCACATTTGCTTTTG+AGG + chr3_2:83578008-83578027 Msa0431530:CDS 35.0%
!! AACAGCACATTTGCTTTTGA+GGG + chr3_2:83578009-83578028 Msa0431530:CDS 35.0%
TGCTTCAGTTTCAATTGCAA+TGG + chr3_2:83578134-83578153 Msa0431530:CDS 35.0%
GCTTCAGTTTCAATTGCAAT+GGG + chr3_2:83578135-83578154 Msa0431530:CDS 35.0%
CTTCAGTTTCAATTGCAATG+GGG + chr3_2:83578136-83578155 Msa0431530:CDS 35.0%
! ATTTTGTCACTACAGAGGAT+TGG + chr3_2:83578208-83578227 Msa0431530:three_prime_UTR 35.0%
!! ACAATTTCACCCCTTTTTCA+GGG - chr3_2:83578444-83578463 None:intergenic 35.0%
! AACAATTTCACCCCTTTTTC+AGG - chr3_2:83578445-83578464 None:intergenic 35.0%
! TTTCTTTGGTAGCATGTGAG+TGG - chr3_2:83575445-83575464 None:intergenic 40.0%
! CAGCACAGTGTGAAAGTTTT+AGG - chr3_2:83575592-83575611 None:intergenic 40.0%
TTCATCCTTTCCTTCCTTCT+TGG + chr3_2:83575619-83575638 Msa0431530:CDS 40.0%
!!! TAGCTGTAGTTTTGGTCACT+TGG + chr3_2:83575842-83575861 Msa0431530:intron 40.0%
! TTTGGTCACTTGGTGGAAAA+TGG + chr3_2:83575852-83575871 Msa0431530:intron 40.0%
! ATGGTGGAGAAAGAAAGTGA+AGG + chr3_2:83575966-83575985 Msa0431530:intron 40.0%
AGTCACAAATTGGTTGTAGG+AGG + chr3_2:83576182-83576201 Msa0431530:intron 40.0%
AAATTGGTTGTAGGAGGACA+GGG + chr3_2:83576188-83576207 Msa0431530:intron 40.0%
GTTAAGGGATAAAGAGTTGG+AGG + chr3_2:83576240-83576259 Msa0431530:intron 40.0%
! CTGCTAGTTTGAAAAAGTGC+CGG - chr3_2:83576398-83576417 None:intergenic 40.0%
GCTATTATACATGGAACACG+TGG - chr3_2:83576473-83576492 None:intergenic 40.0%
! CAGGTCGTTGCTATTATACA+TGG - chr3_2:83576482-83576501 None:intergenic 40.0%
! CATCTTCAAAGGTACATGGT+TGG - chr3_2:83576663-83576682 None:intergenic 40.0%
! GTCTCATCTTCAAAGGTACA+TGG - chr3_2:83576667-83576686 None:intergenic 40.0%
!! TGACACAGATAGAGAGTACT+AGG + chr3_2:83576797-83576816 Msa0431530:intron 40.0%
!! GATTTGGTGTTCGTTAAGAG+TGG + chr3_2:83576834-83576853 Msa0431530:intron 40.0%
CTTCTGCATCAGAGATGAAT+TGG + chr3_2:83576899-83576918 Msa0431530:intron 40.0%
GGTATGCCTTAATGTACTGT+AGG - chr3_2:83577684-83577703 None:intergenic 40.0%
ACAGTACATTAAGGCATACC+AGG + chr3_2:83577684-83577703 Msa0431530:CDS 40.0%
CAGTACATTAAGGCATACCA+GGG + chr3_2:83577685-83577704 Msa0431530:CDS 40.0%
TCAGCAAATTGAGATTCAGG+GGG - chr3_2:83577748-83577767 None:intergenic 40.0%
GCTTCAGCAAATTGAGATTC+AGG - chr3_2:83577751-83577770 None:intergenic 40.0%
ATGCCTTAACACCACAGTTT+GGG - chr3_2:83577780-83577799 None:intergenic 40.0%
TCAACTCTTGCTCGTATAAC+TGG + chr3_2:83577799-83577818 Msa0431530:CDS 40.0%
TCGTATAACTGGTCGTTTCT+TGG + chr3_2:83577810-83577829 Msa0431530:CDS 40.0%
CGTATAACTGGTCGTTTCTT+GGG + chr3_2:83577811-83577830 Msa0431530:CDS 40.0%
GTATAACTGGTCGTTTCTTG+GGG + chr3_2:83577812-83577831 Msa0431530:CDS 40.0%
AGCTCTTCTCGTTTCTTATC+AGG - chr3_2:83577882-83577901 None:intergenic 40.0%
!! TGGTGTGTGTTGTTTAGCAA+TGG + chr3_2:83577979-83577998 Msa0431530:CDS 40.0%
GTGGCTAAAGAAACAAAACC+AGG + chr3_2:83578036-83578055 Msa0431530:CDS 40.0%
ACAAAACCAGGTTGCTATAG+TGG + chr3_2:83578048-83578067 Msa0431530:CDS 40.0%
CCTCTGTAGTGACAAAATGA+AGG - chr3_2:83578206-83578225 None:intergenic 40.0%
! CCTTCATTTTGTCACTACAG+AGG + chr3_2:83578203-83578222 Msa0431530:three_prime_UTR 40.0%
TACCCTATAGCCCTGAAAAA+GGG + chr3_2:83578431-83578450 Msa0431530:three_prime_UTR 40.0%
ACCAAACGTGTGAGTGAGTT+GGG - chr3_2:83575496-83575515 None:intergenic 45.0%
GTGTCTGCTTCAATGTAGTG+AGG - chr3_2:83575526-83575545 None:intergenic 45.0%
AAGCACCAAGAAGGAAGGAA+AGG - chr3_2:83575627-83575646 None:intergenic 45.0%
!! TTCCTTCTTGGTGCTTCTAC+CGG + chr3_2:83575631-83575650 Msa0431530:CDS 45.0%
GGACTTACTTTGAGTAGCAC+CGG - chr3_2:83575653-83575672 None:intergenic 45.0%
!!! CTGTAGTTTTGGTCACTTGG+TGG + chr3_2:83575845-83575864 Msa0431530:intron 45.0%
CAAATTGGTTGTAGGAGGAC+AGG + chr3_2:83576187-83576206 Msa0431530:intron 45.0%
ATTCGTCCGAGTTTGAACTC+AGG - chr3_2:83576267-83576286 None:intergenic 45.0%
!!! CGGCACTTTTTCAAACTAGC+AGG + chr3_2:83576396-83576415 Msa0431530:intron 45.0%
AAAAGAGCAGCAACCAGCAA+CGG - chr3_2:83576987-83577006 None:intergenic 45.0%
TTACATGTGTGCTTCCCAGA+TGG + chr3_2:83577090-83577109 Msa0431530:intron 45.0%
! CAGCTACTAACTTGTCTGCA+GGG - chr3_2:83577591-83577610 None:intergenic 45.0%
CGAAAACAGAACTGCTCACA+AGG + chr3_2:83577618-83577637 Msa0431530:CDS 45.0%
TCTCGCCCTACAGTACATTA+AGG + chr3_2:83577675-83577694 Msa0431530:CDS 45.0%
TGCCTTAACACCACAGTTTG+GGG - chr3_2:83577779-83577798 None:intergenic 45.0%
GATGCCTTAACACCACAGTT+TGG - chr3_2:83577781-83577800 None:intergenic 45.0%
GTGCATGGACATACTTAGTC+TGG - chr3_2:83577840-83577859 None:intergenic 45.0%
TAAGTATGTCCATGCACCTG+AGG + chr3_2:83577843-83577862 Msa0431530:CDS 45.0%
AGGATTTCAGAAAACGCCTC+AGG - chr3_2:83577862-83577881 None:intergenic 45.0%
! ATGGAGGGTCTCCTTATTTC+TGG + chr3_2:83577959-83577978 Msa0431530:CDS 45.0%
!! AGCACATTTGCTTTTGAGGG+TGG + chr3_2:83578012-83578031 Msa0431530:CDS 45.0%
!! ATTTGCTTTTGAGGGTGGTG+TGG + chr3_2:83578017-83578036 Msa0431530:CDS 45.0%
TTAGCTCCACTATAGCAACC+TGG - chr3_2:83578057-83578076 None:intergenic 45.0%
AATAGCCACATCACACTGAC+TGG - chr3_2:83578111-83578130 None:intergenic 45.0%
GTACCCTATAGCCCTGAAAA+AGG + chr3_2:83578430-83578449 Msa0431530:three_prime_UTR 45.0%
ACCCTATAGCCCTGAAAAAG+GGG + chr3_2:83578432-83578451 Msa0431530:three_prime_UTR 45.0%
!!! ACCCCTTTTTCAGGGCTATA+GGG - chr3_2:83578436-83578455 None:intergenic 45.0%
! CTTTGGTAGCATGTGAGTGG+TGG - chr3_2:83575442-83575461 None:intergenic 50.0%
CCAAACGTGTGAGTGAGTTG+GGG - chr3_2:83575495-83575514 None:intergenic 50.0%
CACCAAACGTGTGAGTGAGT+TGG - chr3_2:83575497-83575516 None:intergenic 50.0%
CAACTCACTCACACGTTTGG+TGG + chr3_2:83575495-83575514 Msa0431530:five_prime_UTR 50.0%
!! GGTAGAAGCACCAAGAAGGA+AGG - chr3_2:83575632-83575651 None:intergenic 50.0%
GGAGGTCCTGAGTTCAAACT+CGG + chr3_2:83576258-83576277 Msa0431530:intron 50.0%
GCTAATTATGTGGTCTCCCC+TGG + chr3_2:83576523-83576542 Msa0431530:intron 50.0%
GGCAGAAGAATCCACCATCT+GGG - chr3_2:83577107-83577126 None:intergenic 50.0%
! GCAGCTACTAACTTGTCTGC+AGG - chr3_2:83577592-83577611 None:intergenic 50.0%
AAGGCATACCAGGGTGATTG+TGG + chr3_2:83577694-83577713 Msa0431530:CDS 50.0%
TTGAGATTCAGGGGGCTTCT+TGG - chr3_2:83577740-83577759 None:intergenic 50.0%
! GAAGCTTTTGCCCCAAACTG+TGG + chr3_2:83577766-83577785 Msa0431530:CDS 50.0%
! TGCCCCAAACTGTGGTGTTA+AGG + chr3_2:83577774-83577793 Msa0431530:CDS 50.0%
!! CAAGTTGGTGCTGCTGTAAC+CGG + chr3_2:83577931-83577950 Msa0431530:CDS 50.0%
!! CTGTAACCGGTACTGATGGA+GGG + chr3_2:83577944-83577963 Msa0431530:CDS 50.0%
GGAGCTAATGATGTGTGCAG+TGG + chr3_2:83578069-83578088 Msa0431530:CDS 50.0%
TGTGCAGTGGTGCTCATGTT+TGG + chr3_2:83578082-83578101 Msa0431530:CDS 50.0%
!!! CACCCCTTTTTCAGGGCTAT+AGG - chr3_2:83578437-83578456 None:intergenic 50.0%
CCCCAACTCACTCACACGTT+TGG + chr3_2:83575492-83575511 Msa0431530:five_prime_UTR 55.0%
!! CACCGGTAGAAGCACCAAGA+AGG - chr3_2:83575636-83575655 None:intergenic 55.0%
TTGGTTGTAGGAGGACAGGG+CGG + chr3_2:83576191-83576210 Msa0431530:intron 55.0%
GGAGGGTTGGGTAGTTCAAC+TGG + chr3_2:83576212-83576231 Msa0431530:intron 55.0%
CATGTGTGCTTCCCAGATGG+TGG + chr3_2:83577093-83577112 Msa0431530:intron 55.0%
CGGCAGAAGAATCCACCATC+TGG - chr3_2:83577108-83577127 None:intergenic 55.0%
!! CAGGGTGATTGTGGTGCTGT+GGG + chr3_2:83577703-83577722 Msa0431530:CDS 55.0%
CAGAAAACGCCTCAGGTGCA+TGG - chr3_2:83577855-83577874 None:intergenic 55.0%
! GCTGCTGTAACCGGTACTGA+TGG + chr3_2:83577940-83577959 Msa0431530:CDS 55.0%
!! GCTGTAACCGGTACTGATGG+AGG + chr3_2:83577943-83577962 Msa0431530:CDS 55.0%
AGGAGACCCTCCATCAGTAC+CGG - chr3_2:83577953-83577972 None:intergenic 55.0%
GGTCACCAGTCAGTGTGATG+TGG + chr3_2:83578103-83578122 Msa0431530:CDS 55.0%
TTGTAGGAGGACAGGGCGGA+GGG + chr3_2:83576195-83576214 Msa0431530:intron 60.0%
CCACAGCACCACAATCACCC+TGG - chr3_2:83577705-83577724 None:intergenic 60.0%
! CCAGGGTGATTGTGGTGCTG+TGG + chr3_2:83577702-83577721 Msa0431530:CDS 60.0%
!! GGTGATTGTGGTGCTGTGGG+TGG + chr3_2:83577706-83577725 Msa0431530:CDS 60.0%
GTTGTAGGAGGACAGGGCGG+AGG + chr3_2:83576194-83576213 Msa0431530:intron 65.0%
TAGGGCGAGACATGCGAGCC+CGG - chr3_2:83577665-83577684 None:intergenic 65.0%
AGGAGGACAGGGCGGAGGGT+TGG + chr3_2:83576199-83576218 Msa0431530:intron 70.0%
GGAGGACAGGGCGGAGGGTT+GGG + chr3_2:83576200-83576219 Msa0431530:intron 70.0%
AGGGCGAGACATGCGAGCCC+GGG - chr3_2:83577664-83577683 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 83575401 83578524 83575401 ID=Msa0431530;Name=Msa0431530
chr3_2 mRNA 83575401 83578524 83575401 ID=Msa0431530-mRNA-1;Parent=Msa0431530;Name=Msa0431530-mRNA-1;_AED=0.00;_eAED=0.00;_QI=185|1|1|1|1|1|2|348|227
chr3_2 exon 83575401 83575664 83575401 ID=Msa0431530-mRNA-1:exon:23358;Parent=Msa0431530-mRNA-1
chr3_2 exon 83577572 83578524 83577572 ID=Msa0431530-mRNA-1:exon:23359;Parent=Msa0431530-mRNA-1
chr3_2 five_prime_UTR 83575401 83575585 83575401 ID=Msa0431530-mRNA-1:five_prime_utr;Parent=Msa0431530-mRNA-1
chr3_2 CDS 83575586 83575664 83575586 ID=Msa0431530-mRNA-1:cds;Parent=Msa0431530-mRNA-1
chr3_2 CDS 83577572 83578176 83577572 ID=Msa0431530-mRNA-1:cds;Parent=Msa0431530-mRNA-1
chr3_2 three_prime_UTR 83578177 83578524 83578177 ID=Msa0431530-mRNA-1:three_prime_utr;Parent=Msa0431530-mRNA-1
Gene Sequence

>Msa0431530

ATGCCTAAAACTTTCACACTGTGCTGTCTTTTCTTCATCCTTTCCTTCCTTCTTGGTGCTTCTACCGGTGCTACTCAAATCAAAGTTACTGATAACCCTGCAGACAAGTTAGTAGCTGCTATCAACGAAAACAGAACTGCTCACAAGGATTCATCTTTATTTGACAATCCCGGGCTCGCATGTCTCGCCCTACAGTACATTAAGGCATACCAGGGTGATTGTGGTGCTGTGGGTGGATCTGATGCCAAGAAGCCCCCTGAATCTCAATTTGCTGAAGCTTTTGCCCCAAACTGTGGTGTTAAGGCATCAACTCTTGCTCGTATAACTGGTCGTTTCTTGGGGTGCCAGACTAAGTATGTCCATGCACCTGAGGCGTTTTCTGAAATCCTGATAAGAAACGAGAAGAGCTTAGACATACTCTACAGTAAGAATCACACTCAAGTTGGTGCTGCTGTAACCGGTACTGATGGAGGGTCTCCTTATTTCTGGTGTGTGTTGTTTAGCAATGGCAAACCTAACAGCACATTTGCTTTTGAGGGTGGTGTGGCTAAAGAAACAAAACCAGGTTGCTATAGTGGAGCTAATGATGTGTGCAGTGGTGCTCATGTTTGGTCACCAGTCAGTGTGATGTGGCTATTTGTTGCTTCAGTTTCAATTGCAATGGGGTTTGCTTTTCCATTGTGA
Protein sequence

>Msa0431530

MPKTFTLCCLFFILSFLLGASTGATQIKVTDNPADKLVAAINENRTAHKDSSLFDNPGLACLALQYIKAYQGDCGAVGGSDAKKPPESQFAEAFAPNCGVKASTLARITGRFLGCQTKYVHAPEAFSEILIRNEKSLDILYSKNHTQVGAAVTGTDGGSPYFWCVLFSNGKPNSTFAFEGGVAKETKPGCYSGANDVCSGAHVWSPVSVMWLFVASVSIAMGFAFPL*