AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0382030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0382030 MtrunA17_Chr3g0136301 96.507 229 6 1 1 227 1 229 4.17e-165 454
Msa0382030 MtrunA17_Chr8g0336911 45.026 191 103 2 32 221 30 219 2.28e-54 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0382030 AT1G02180.1 44.000 175 92 6 32 203 29 200 3.77e-43 145

Find 78 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAGGGTCTCCTTATTTC+TGG 0.118257 3_1:+84505683 Msa0382030:CDS
TTCATCCTTTCCTTCCTTCT+TGG 0.129768 3_1:+84503344 Msa0382030:CDS
AAGTACCTTCTTAAAGATTC+TGG 0.222866 3_1:-84503138 None:intergenic
TCGTATAACTGGTCGTTTCT+TGG 0.250063 3_1:+84505534 Msa0382030:CDS
GCTTCAGCAAATTGAGATTC+AGG 0.261524 3_1:-84505472 None:intergenic
AGCTCTTCTCGTTTCTTATC+AGG 0.274033 3_1:-84505603 None:intergenic
CGTATAACTGGTCGTTTCTT+GGG 0.276791 3_1:+84505535 Msa0382030:CDS
GTACCCTATAGCCCTGAAAA+AGG 0.292646 3_1:+84506154 Msa0382030:three_prime_UTR
ATGCCTTAACACCACAGTTT+GGG 0.323486 3_1:-84505501 None:intergenic
ATTCAAGATGCATTTGGTTA+TGG 0.323548 3_1:+84506128 Msa0382030:three_prime_UTR
AACAACACACACCAGAAATA+AGG 0.333485 3_1:-84505694 None:intergenic
ACCAAACGTGTGAGTGAGTT+GGG 0.348682 3_1:-84503218 None:intergenic
GATGCCTTAACACCACAGTT+TGG 0.357391 3_1:-84505502 None:intergenic
TCAACTCTTGCTCGTATAAC+TGG 0.364017 3_1:+84505523 Msa0382030:CDS
TGCCCCAAACTGTGGTGTTA+AGG 0.376413 3_1:+84505498 Msa0382030:CDS
TCAAAAGCAAATGTGCTGTT+AGG 0.377113 3_1:-84505730 None:intergenic
GCATATGAAACATGTGATAT+TGG 0.378061 3_1:+84506047 Msa0382030:three_prime_UTR
GCTTCAGTTTCAATTGCAAT+GGG 0.382192 3_1:+84505859 Msa0382030:CDS
ATTGGAATTCAAGATGCATT+TGG 0.388458 3_1:+84506122 Msa0382030:three_prime_UTR
CAAGTTGGTGCTGCTGTAAC+CGG 0.391700 3_1:+84505655 Msa0382030:CDS
TTCCTTCTTGGTGCTTCTAC+CGG 0.398930 3_1:+84503356 Msa0382030:CDS
ACAAAACCAGGTTGCTATAG+TGG 0.403030 3_1:+84505772 Msa0382030:CDS
TGCTTCAGTTTCAATTGCAA+TGG 0.413253 3_1:+84505858 Msa0382030:CDS
TGCTTTGATTGTTATACGAT+TGG 0.413715 3_1:+84506104 Msa0382030:three_prime_UTR
TCTCGCCCTACAGTACATTA+AGG 0.413855 3_1:+84505399 Msa0382030:CDS
CCCCAACTCACTCACACGTT+TGG 0.438855 3_1:+84503217 Msa0382030:five_prime_UTR
TCATCTTTATTTGACAATCC+CGG 0.454811 3_1:+84505367 Msa0382030:CDS
TGTGCAGTGGTGCTCATGTT+TGG 0.457962 3_1:+84505806 Msa0382030:CDS
CAACTCACTCACACGTTTGG+TGG 0.462241 3_1:+84503220 Msa0382030:five_prime_UTR
ATATCACATGTTTCATATGC+AGG 0.471229 3_1:-84506044 None:intergenic
TAGGGCGAGACATGCGAGCC+CGG 0.474850 3_1:-84505386 None:intergenic
GCAGCTACTAACTTGTCTGC+AGG 0.476484 3_1:-84505313 None:intergenic
CATCTTTATTTGACAATCCC+GGG 0.500468 3_1:+84505368 Msa0382030:CDS
GGTGATTGTGGTGCTGTGGG+TGG 0.502206 3_1:+84505430 Msa0382030:CDS
GGTATGCCTTAATGTACTGT+AGG 0.503759 3_1:-84505405 None:intergenic
CAGGGTGATTGTGGTGCTGT+GGG 0.510763 3_1:+84505427 Msa0382030:CDS
CTTCAGCAAATTGAGATTCA+GGG 0.511447 3_1:-84505471 None:intergenic
CGAAAACAGAACTGCTCACA+AGG 0.516931 3_1:+84505342 Msa0382030:CDS
GTGCATGGACATACTTAGTC+TGG 0.524594 3_1:-84505561 None:intergenic
GTATAACTGGTCGTTTCTTG+GGG 0.529253 3_1:+84505536 Msa0382030:CDS
TGGTGTGTGTTGTTTAGCAA+TGG 0.533016 3_1:+84505703 Msa0382030:CDS
CCAGGGTGATTGTGGTGCTG+TGG 0.539111 3_1:+84505426 Msa0382030:CDS
AATAGCCACATCACACTGAC+TGG 0.539928 3_1:-84505832 None:intergenic
TACTTCCAGAATCTTTAAGA+AGG 0.542813 3_1:+84503133 Msa0382030:five_prime_UTR
CACCAAACGTGTGAGTGAGT+TGG 0.545998 3_1:-84503219 None:intergenic
AAGGCATACCAGGGTGATTG+TGG 0.548506 3_1:+84505418 Msa0382030:CDS
ACAGTACATTAAGGCATACC+AGG 0.557681 3_1:+84505408 Msa0382030:CDS
CAGAAAACGCCTCAGGTGCA+TGG 0.559426 3_1:-84505576 None:intergenic
GCTGCTGTAACCGGTACTGA+TGG 0.569000 3_1:+84505664 Msa0382030:CDS
AGTAAGAATCACACTCAAGT+TGG 0.576187 3_1:+84505640 Msa0382030:CDS
AAGCACCAAGAAGGAAGGAA+AGG 0.578158 3_1:-84503349 None:intergenic
AGGGCGAGACATGCGAGCCC+GGG 0.579446 3_1:-84505385 None:intergenic
TGCCTTAACACCACAGTTTG+GGG 0.580489 3_1:-84505500 None:intergenic
CTTTGGTAGCATGTGAGTGG+TGG 0.580649 3_1:-84503164 None:intergenic
CACCGGTAGAAGCACCAAGA+AGG 0.580761 3_1:-84503358 None:intergenic
GCTGTAACCGGTACTGATGG+AGG 0.581331 3_1:+84505667 Msa0382030:CDS
AGGATTTCAGAAAACGCCTC+AGG 0.582162 3_1:-84505583 None:intergenic
CCTCTGTAGTGACAAAATGA+AGG 0.583962 3_1:-84505927 None:intergenic
GGTAGAAGCACCAAGAAGGA+AGG 0.585888 3_1:-84503354 None:intergenic
AGGAGACCCTCCATCAGTAC+CGG 0.597918 3_1:-84505674 None:intergenic
GTATGCCTTAATGTACTGTA+GGG 0.599616 3_1:-84505404 None:intergenic
TTTCTTTGGTAGCATGTGAG+TGG 0.604521 3_1:-84503167 None:intergenic
TTCAGCAAATTGAGATTCAG+GGG 0.606803 3_1:-84505470 None:intergenic
GGACTTACTTTGAGTAGCAC+CGG 0.613594 3_1:-84503375 None:intergenic
GGTCACCAGTCAGTGTGATG+TGG 0.613666 3_1:+84505827 Msa0382030:CDS
CAGCTACTAACTTGTCTGCA+GGG 0.614123 3_1:-84505312 None:intergenic
CTTCAGTTTCAATTGCAATG+GGG 0.618866 3_1:+84505860 Msa0382030:CDS
CTGTAACCGGTACTGATGGA+GGG 0.623210 3_1:+84505668 Msa0382030:CDS
AAATAACAAAATCAAAACAA+AGG 0.645136 3_1:-84505982 None:intergenic
TTAGCTCCACTATAGCAACC+TGG 0.653166 3_1:-84505778 None:intergenic
CCAAACGTGTGAGTGAGTTG+GGG 0.654948 3_1:-84503217 None:intergenic
TCAGCAAATTGAGATTCAGG+GGG 0.657704 3_1:-84505469 None:intergenic
CCACAGCACCACAATCACCC+TGG 0.672377 3_1:-84505426 None:intergenic
GTGGCTAAAGAAACAAAACC+AGG 0.685787 3_1:+84505760 Msa0382030:CDS
TAAGTATGTCCATGCACCTG+AGG 0.686474 3_1:+84505567 Msa0382030:CDS
GTGTCTGCTTCAATGTAGTG+AGG 0.687007 3_1:-84503248 None:intergenic
CAGTACATTAAGGCATACCA+GGG 0.710618 3_1:+84505409 Msa0382030:CDS
GGAGCTAATGATGTGTGCAG+TGG 0.715652 3_1:+84505793 Msa0382030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCATTGATTATATATA+TGG + chr3_1:84505066-84505085 Msa0382030:intron 10.0%
!!! AATAAAAAAAAGACTTGTTT+TGG - chr3_1:84503399-84503418 None:intergenic 15.0%
!!! TTTATTTTATATCTCAGATT+TGG + chr3_1:84503413-84503432 Msa0382030:intron 15.0%
!! CATTTGTCATTAAAAAAAAT+TGG + chr3_1:84504044-84504063 Msa0382030:intron 15.0%
!! AGAAAATAAATATAACTATG+AGG - chr3_1:84505182-84505201 None:intergenic 15.0%
!! AAATAACAAAATCAAAACAA+AGG - chr3_1:84505985-84506004 None:intergenic 15.0%
!!! TGTTTTTTTCTTTCTTTCTT+TGG - chr3_1:84503184-84503203 None:intergenic 20.0%
!! CTATAACTCATTAAAGTTTA+TGG + chr3_1:84503779-84503798 Msa0382030:intron 20.0%
!! CATTAAAAAAAATTGGTTGT+AGG + chr3_1:84504051-84504070 Msa0382030:intron 20.0%
!! GACAATATAAAATGAAATCA+AGG - chr3_1:84504146-84504165 None:intergenic 20.0%
!! AAAAAAATTGAACTGTTATG+TGG + chr3_1:84504327-84504346 Msa0382030:intron 20.0%
!! AAATATGTAACCTTAATCAA+AGG - chr3_1:84505042-84505061 None:intergenic 20.0%
!! ATAAATATAACTATGAGGTA+GGG - chr3_1:84505177-84505196 None:intergenic 20.0%
!! AATAAATATAACTATGAGGT+AGG - chr3_1:84505178-84505197 None:intergenic 20.0%
!!! TGAATTTTTACTTGATTTAG+TGG + chr3_1:84505904-84505923 Msa0382030:three_prime_UTR 20.0%
! TCTTGCAAGTTGTATTAAAA+TGG + chr3_1:84503623-84503642 Msa0382030:intron 25.0%
! TGAAAGTTCCAAATAAGATA+CGG - chr3_1:84503666-84503685 None:intergenic 25.0%
!! ATTTGGAACTTTCACTATAA+TGG + chr3_1:84503672-84503691 Msa0382030:intron 25.0%
! TATGTTAGTTAGTCACAAAT+TGG + chr3_1:84503897-84503916 Msa0382030:intron 25.0%
! TTAAACAATAAGCATCAAAC+GGG - chr3_1:84504225-84504244 None:intergenic 25.0%
! CTTAAACAATAAGCATCAAA+CGG - chr3_1:84504226-84504245 None:intergenic 25.0%
! TTGTTTAAGAGCTAATTATG+TGG + chr3_1:84504237-84504256 Msa0382030:intron 25.0%
! TTTCAACAAAATTAATCCAG+GGG - chr3_1:84504266-84504285 None:intergenic 25.0%
! CTTTCAACAAAATTAATCCA+GGG - chr3_1:84504267-84504286 None:intergenic 25.0%
! TCTTTCAACAAAATTAATCC+AGG - chr3_1:84504268-84504287 None:intergenic 25.0%
!! ATGACTTTTGTAGTGAAATT+AGG + chr3_1:84504457-84504476 Msa0382030:intron 25.0%
! TGAAATTAGGACTAAACTTA+TGG + chr3_1:84504470-84504489 Msa0382030:intron 25.0%
!!! TGAATATCTAGCTTTCATTA+TGG + chr3_1:84504600-84504619 Msa0382030:intron 25.0%
! AGACAAACTATAACAAATGT+TGG - chr3_1:84504786-84504805 None:intergenic 25.0%
!!! ACATTTGTTATAGTTTGTCT+AGG + chr3_1:84504786-84504805 Msa0382030:intron 25.0%
! AACCTTAATCAAAGGATAAA+CGG - chr3_1:84505034-84505053 None:intergenic 25.0%
! GTAAAAATTCATGATCACAA+TGG - chr3_1:84505895-84505914 None:intergenic 25.0%
!!! TGATTTTGTTATTTGTTGAG+AGG + chr3_1:84505991-84506010 Msa0382030:three_prime_UTR 25.0%
!! AAATTGTTGCATAAGCTTTT+TGG + chr3_1:84506181-84506200 Msa0382030:three_prime_UTR 25.0%
! TACTTCCAGAATCTTTAAGA+AGG + chr3_1:84503133-84503152 Msa0382030:five_prime_UTR 30.0%
AAGTACCTTCTTAAAGATTC+TGG - chr3_1:84503141-84503160 None:intergenic 30.0%
TATGTAACATGAGTTTGATC+TGG + chr3_1:84503454-84503473 Msa0382030:intron 30.0%
ATTACTGTGAAATGCTACAT+TGG - chr3_1:84503542-84503561 None:intergenic 30.0%
AGGAAAAAGAGAACAAGTTA+AGG + chr3_1:84503711-84503730 Msa0382030:intron 30.0%
ATTGTCCAAATCAAGAATCA+CGG - chr3_1:84503742-84503761 None:intergenic 30.0%
!!! TTATGGTCTTTGTTTTAGTG+TGG + chr3_1:84503796-84503815 Msa0382030:intron 30.0%
TTGTGACTAACTAACATAAG+TGG - chr3_1:84503895-84503914 None:intergenic 30.0%
TTTATAACTGTATACGCTTC+CGG + chr3_1:84504100-84504119 Msa0382030:intron 30.0%
! CATTTTATATTGTCTCTGCT+TGG + chr3_1:84504152-84504171 Msa0382030:intron 30.0%
AATTGAACTGTTATGTGGTT+AGG + chr3_1:84504332-84504351 Msa0382030:intron 30.0%
ACAAAAGTCATAACTTTGCA+TGG - chr3_1:84504448-84504467 None:intergenic 30.0%
AAACTTATGGAAGCCTTAAA+TGG + chr3_1:84504483-84504502 Msa0382030:intron 30.0%
! GGATTTAGGAATTTGAGATT+TGG + chr3_1:84504542-84504561 Msa0382030:intron 30.0%
TATAGACTAAACTCTGTCTT+TGG - chr3_1:84504658-84504677 None:intergenic 30.0%
ACAGAGTTTAGTCTATACAA+AGG + chr3_1:84504661-84504680 Msa0382030:intron 30.0%
CAGAGTTTAGTCTATACAAA+GGG + chr3_1:84504662-84504681 Msa0382030:intron 30.0%
AAGATAAATAAAACCGTTGC+TGG + chr3_1:84504695-84504714 Msa0382030:intron 30.0%
ACAATGATAGAAGCAATATC+AGG - chr3_1:84504749-84504768 None:intergenic 30.0%
TAGTGACTGTATTTCAAACA+CGG + chr3_1:84504975-84504994 Msa0382030:intron 30.0%
TAATCAAAGGATAAACGGAA+AGG - chr3_1:84505029-84505048 None:intergenic 30.0%
TTCCGTTTATCCTTTGATTA+AGG + chr3_1:84505029-84505048 Msa0382030:intron 30.0%
AATTTAGAGAACACACAAGT+GGG - chr3_1:84505244-84505263 None:intergenic 30.0%
TAATTTAGAGAACACACAAG+TGG - chr3_1:84505245-84505264 None:intergenic 30.0%
! TCATCTTTATTTGACAATCC+CGG + chr3_1:84505367-84505386 Msa0382030:CDS 30.0%
ATATCACATGTTTCATATGC+AGG - chr3_1:84506047-84506066 None:intergenic 30.0%
GCATATGAAACATGTGATAT+TGG + chr3_1:84506047-84506066 Msa0382030:three_prime_UTR 30.0%
! CTCTTGTAATTTTCTCATCT+AGG + chr3_1:84506070-84506089 Msa0382030:three_prime_UTR 30.0%
TGCTTTGATTGTTATACGAT+TGG + chr3_1:84506104-84506123 Msa0382030:three_prime_UTR 30.0%
ATTGGAATTCAAGATGCATT+TGG + chr3_1:84506122-84506141 Msa0382030:three_prime_UTR 30.0%
ATTCAAGATGCATTTGGTTA+TGG + chr3_1:84506128-84506147 Msa0382030:three_prime_UTR 30.0%
! AATTCTGCTAGCTGTAGTTT+TGG + chr3_1:84503559-84503578 Msa0382030:intron 35.0%
AGAACTGTCCGTATCTTATT+TGG + chr3_1:84503655-84503674 Msa0382030:intron 35.0%
TGGAACTTTCACTATAATGG+TGG + chr3_1:84503675-84503694 Msa0382030:intron 35.0%
ATATCCCGTGATTCTTGATT+TGG + chr3_1:84503734-84503753 Msa0382030:intron 35.0%
TTGTCCAAATCAAGAATCAC+GGG - chr3_1:84503741-84503760 None:intergenic 35.0%
GAGTTATAGAGACGAAGAAA+TGG - chr3_1:84503768-84503787 None:intergenic 35.0%
!! GTTTTAGTGTGGAGTTTCAT+TGG + chr3_1:84503807-84503826 Msa0382030:intron 35.0%
GTTAGTCACAAATTGGTTGT+AGG + chr3_1:84503904-84503923 Msa0382030:intron 35.0%
!! AGTTCAACTGGTTTGAGTTA+AGG + chr3_1:84503949-84503968 Msa0382030:intron 35.0%
!! GTTCAACTGGTTTGAGTTAA+GGG + chr3_1:84503950-84503969 Msa0382030:intron 35.0%
TGAGTTAAGGGATAAAGAGT+TGG + chr3_1:84503962-84503981 Msa0382030:intron 35.0%
! ACGTGGACATAGTTCATTTT+TGG - chr3_1:84504180-84504199 None:intergenic 35.0%
CTAGAGCTAGAAACATAGAT+AGG - chr3_1:84504306-84504325 None:intergenic 35.0%
!! CTATCTATGTTTCTAGCTCT+AGG + chr3_1:84504304-84504323 Msa0382030:intron 35.0%
AACAGAAGTCTCATCTTCAA+AGG - chr3_1:84504398-84504417 None:intergenic 35.0%
AAAGTCATAACTTTGCATGG+TGG - chr3_1:84504445-84504464 None:intergenic 35.0%
TACAAAGAGGTCACCATTTA+AGG - chr3_1:84504499-84504518 None:intergenic 35.0%
TATCTGTGTCAAGTACAAAG+AGG - chr3_1:84504512-84504531 None:intergenic 35.0%
!! GATAGAGAGTACTAGGATTT+AGG + chr3_1:84504528-84504547 Msa0382030:intron 35.0%
TAAGAGTGGAGAAAATGTTG+TGG + chr3_1:84504572-84504591 Msa0382030:intron 35.0%
ATGATAGAAGCAATATCAGG+AGG - chr3_1:84504746-84504765 None:intergenic 35.0%
TGAGTTTCTCAACAGATTCT+CGG - chr3_1:84504852-84504871 None:intergenic 35.0%
AAAGTAGGCTGTTTATTGTG+AGG - chr3_1:84505099-84505118 None:intergenic 35.0%
! CACAATAAACAGCCTACTTT+TGG + chr3_1:84505099-84505118 Msa0382030:intron 35.0%
!! TTTTTCAGTGAGCCAAAAGT+AGG - chr3_1:84505114-84505133 None:intergenic 35.0%
!!! TACTTTTGGCTCACTGAAAA+AGG + chr3_1:84505113-84505132 Msa0382030:intron 35.0%
!!! ACTTTTGGCTCACTGAAAAA+GGG + chr3_1:84505114-84505133 Msa0382030:intron 35.0%
! CATCTTTATTTGACAATCCC+GGG + chr3_1:84505368-84505387 Msa0382030:CDS 35.0%
GTATGCCTTAATGTACTGTA+GGG - chr3_1:84505407-84505426 None:intergenic 35.0%
TTCAGCAAATTGAGATTCAG+GGG - chr3_1:84505473-84505492 None:intergenic 35.0%
CTTCAGCAAATTGAGATTCA+GGG - chr3_1:84505474-84505493 None:intergenic 35.0%
AGTAAGAATCACACTCAAGT+TGG + chr3_1:84505640-84505659 Msa0382030:CDS 35.0%
AACAACACACACCAGAAATA+AGG - chr3_1:84505697-84505716 None:intergenic 35.0%
! TCAAAAGCAAATGTGCTGTT+AGG - chr3_1:84505733-84505752 None:intergenic 35.0%
!! TAACAGCACATTTGCTTTTG+AGG + chr3_1:84505732-84505751 Msa0382030:CDS 35.0%
!! AACAGCACATTTGCTTTTGA+GGG + chr3_1:84505733-84505752 Msa0382030:CDS 35.0%
TGCTTCAGTTTCAATTGCAA+TGG + chr3_1:84505858-84505877 Msa0382030:CDS 35.0%
GCTTCAGTTTCAATTGCAAT+GGG + chr3_1:84505859-84505878 Msa0382030:CDS 35.0%
CTTCAGTTTCAATTGCAATG+GGG + chr3_1:84505860-84505879 Msa0382030:CDS 35.0%
! ATTTTGTCACTACAGAGGAT+TGG + chr3_1:84505932-84505951 Msa0382030:three_prime_UTR 35.0%
!! ACAATTTCACCCCTTTTTCA+GGG - chr3_1:84506168-84506187 None:intergenic 35.0%
! AACAATTTCACCCCTTTTTC+AGG - chr3_1:84506169-84506188 None:intergenic 35.0%
! TTTCTTTGGTAGCATGTGAG+TGG - chr3_1:84503170-84503189 None:intergenic 40.0%
! CAGCACAGTGTGAAAGTTTT+AGG - chr3_1:84503317-84503336 None:intergenic 40.0%
TTCATCCTTTCCTTCCTTCT+TGG + chr3_1:84503344-84503363 Msa0382030:CDS 40.0%
!!! TAGCTGTAGTTTTGGTCACT+TGG + chr3_1:84503567-84503586 Msa0382030:intron 40.0%
! TTTGGTCACTTGGTGGAAAA+TGG + chr3_1:84503577-84503596 Msa0382030:intron 40.0%
! ATGGTGGAGAAAGAAAGTGA+AGG + chr3_1:84503691-84503710 Msa0382030:intron 40.0%
AGTCACAAATTGGTTGTAGG+AGG + chr3_1:84503907-84503926 Msa0382030:intron 40.0%
AAATTGGTTGTAGGAGGACA+GGG + chr3_1:84503913-84503932 Msa0382030:intron 40.0%
GTTAAGGGATAAAGAGTTGG+AGG + chr3_1:84503965-84503984 Msa0382030:intron 40.0%
! CTGCTAGTTTGAAAAAGTGC+CGG - chr3_1:84504122-84504141 None:intergenic 40.0%
GCTATTATACATGGAACACG+TGG - chr3_1:84504197-84504216 None:intergenic 40.0%
! CATCTTCAAAGGTACATGGT+TGG - chr3_1:84504387-84504406 None:intergenic 40.0%
! GTCTCATCTTCAAAGGTACA+TGG - chr3_1:84504391-84504410 None:intergenic 40.0%
!! TGACACAGATAGAGAGTACT+AGG + chr3_1:84504521-84504540 Msa0382030:intron 40.0%
!! GATTTGGTGTTCGTTAAGAG+TGG + chr3_1:84504558-84504577 Msa0382030:intron 40.0%
CTTCTGCATCAGAGATGAAT+TGG + chr3_1:84504623-84504642 Msa0382030:intron 40.0%
GGTATGCCTTAATGTACTGT+AGG - chr3_1:84505408-84505427 None:intergenic 40.0%
ACAGTACATTAAGGCATACC+AGG + chr3_1:84505408-84505427 Msa0382030:CDS 40.0%
CAGTACATTAAGGCATACCA+GGG + chr3_1:84505409-84505428 Msa0382030:CDS 40.0%
TCAGCAAATTGAGATTCAGG+GGG - chr3_1:84505472-84505491 None:intergenic 40.0%
GCTTCAGCAAATTGAGATTC+AGG - chr3_1:84505475-84505494 None:intergenic 40.0%
ATGCCTTAACACCACAGTTT+GGG - chr3_1:84505504-84505523 None:intergenic 40.0%
TCAACTCTTGCTCGTATAAC+TGG + chr3_1:84505523-84505542 Msa0382030:CDS 40.0%
TCGTATAACTGGTCGTTTCT+TGG + chr3_1:84505534-84505553 Msa0382030:CDS 40.0%
CGTATAACTGGTCGTTTCTT+GGG + chr3_1:84505535-84505554 Msa0382030:CDS 40.0%
GTATAACTGGTCGTTTCTTG+GGG + chr3_1:84505536-84505555 Msa0382030:CDS 40.0%
AGCTCTTCTCGTTTCTTATC+AGG - chr3_1:84505606-84505625 None:intergenic 40.0%
!! TGGTGTGTGTTGTTTAGCAA+TGG + chr3_1:84505703-84505722 Msa0382030:CDS 40.0%
GTGGCTAAAGAAACAAAACC+AGG + chr3_1:84505760-84505779 Msa0382030:CDS 40.0%
ACAAAACCAGGTTGCTATAG+TGG + chr3_1:84505772-84505791 Msa0382030:CDS 40.0%
CCTCTGTAGTGACAAAATGA+AGG - chr3_1:84505930-84505949 None:intergenic 40.0%
! CCTTCATTTTGTCACTACAG+AGG + chr3_1:84505927-84505946 Msa0382030:three_prime_UTR 40.0%
TACCCTATAGCCCTGAAAAA+GGG + chr3_1:84506155-84506174 Msa0382030:three_prime_UTR 40.0%
ACCAAACGTGTGAGTGAGTT+GGG - chr3_1:84503221-84503240 None:intergenic 45.0%
GTGTCTGCTTCAATGTAGTG+AGG - chr3_1:84503251-84503270 None:intergenic 45.0%
AAGCACCAAGAAGGAAGGAA+AGG - chr3_1:84503352-84503371 None:intergenic 45.0%
!! TTCCTTCTTGGTGCTTCTAC+CGG + chr3_1:84503356-84503375 Msa0382030:CDS 45.0%
GGACTTACTTTGAGTAGCAC+CGG - chr3_1:84503378-84503397 None:intergenic 45.0%
!!! CTGTAGTTTTGGTCACTTGG+TGG + chr3_1:84503570-84503589 Msa0382030:intron 45.0%
CAAATTGGTTGTAGGAGGAC+AGG + chr3_1:84503912-84503931 Msa0382030:intron 45.0%
ATTCGTCCGAGTTTGAACTC+AGG - chr3_1:84503992-84504011 None:intergenic 45.0%
!!! CGGCACTTTTTCAAACTAGC+AGG + chr3_1:84504120-84504139 Msa0382030:intron 45.0%
! CGGGTCGTTGCTATTATACA+TGG - chr3_1:84504206-84504225 None:intergenic 45.0%
AAAAGAGCAGCAACCAGCAA+CGG - chr3_1:84504711-84504730 None:intergenic 45.0%
TTACATGTGTGCTTCCCAGA+TGG + chr3_1:84504814-84504833 Msa0382030:intron 45.0%
! CAGCTACTAACTTGTCTGCA+GGG - chr3_1:84505315-84505334 None:intergenic 45.0%
CGAAAACAGAACTGCTCACA+AGG + chr3_1:84505342-84505361 Msa0382030:CDS 45.0%
TCTCGCCCTACAGTACATTA+AGG + chr3_1:84505399-84505418 Msa0382030:CDS 45.0%
TGCCTTAACACCACAGTTTG+GGG - chr3_1:84505503-84505522 None:intergenic 45.0%
GATGCCTTAACACCACAGTT+TGG - chr3_1:84505505-84505524 None:intergenic 45.0%
GTGCATGGACATACTTAGTC+TGG - chr3_1:84505564-84505583 None:intergenic 45.0%
TAAGTATGTCCATGCACCTG+AGG + chr3_1:84505567-84505586 Msa0382030:CDS 45.0%
AGGATTTCAGAAAACGCCTC+AGG - chr3_1:84505586-84505605 None:intergenic 45.0%
! ATGGAGGGTCTCCTTATTTC+TGG + chr3_1:84505683-84505702 Msa0382030:CDS 45.0%
!! AGCACATTTGCTTTTGAGGG+TGG + chr3_1:84505736-84505755 Msa0382030:CDS 45.0%
!! ATTTGCTTTTGAGGGTGGTG+TGG + chr3_1:84505741-84505760 Msa0382030:CDS 45.0%
TTAGCTCCACTATAGCAACC+TGG - chr3_1:84505781-84505800 None:intergenic 45.0%
AATAGCCACATCACACTGAC+TGG - chr3_1:84505835-84505854 None:intergenic 45.0%
GTACCCTATAGCCCTGAAAA+AGG + chr3_1:84506154-84506173 Msa0382030:three_prime_UTR 45.0%
ACCCTATAGCCCTGAAAAAG+GGG + chr3_1:84506156-84506175 Msa0382030:three_prime_UTR 45.0%
!!! ACCCCTTTTTCAGGGCTATA+GGG - chr3_1:84506160-84506179 None:intergenic 45.0%
! CTTTGGTAGCATGTGAGTGG+TGG - chr3_1:84503167-84503186 None:intergenic 50.0%
CCAAACGTGTGAGTGAGTTG+GGG - chr3_1:84503220-84503239 None:intergenic 50.0%
CACCAAACGTGTGAGTGAGT+TGG - chr3_1:84503222-84503241 None:intergenic 50.0%
CAACTCACTCACACGTTTGG+TGG + chr3_1:84503220-84503239 Msa0382030:five_prime_UTR 50.0%
!! GGTAGAAGCACCAAGAAGGA+AGG - chr3_1:84503357-84503376 None:intergenic 50.0%
GGAGGTCCTGAGTTCAAACT+CGG + chr3_1:84503983-84504002 Msa0382030:intron 50.0%
GCTAATTATGTGGTCTCCCC+TGG + chr3_1:84504247-84504266 Msa0382030:intron 50.0%
GGCAGAAGAATCCACCATCT+GGG - chr3_1:84504831-84504850 None:intergenic 50.0%
! GCAGCTACTAACTTGTCTGC+AGG - chr3_1:84505316-84505335 None:intergenic 50.0%
AAGGCATACCAGGGTGATTG+TGG + chr3_1:84505418-84505437 Msa0382030:CDS 50.0%
TTGAGATTCAGGGGGCTTCT+TGG - chr3_1:84505464-84505483 None:intergenic 50.0%
! GAAGCTTTTGCCCCAAACTG+TGG + chr3_1:84505490-84505509 Msa0382030:CDS 50.0%
! TGCCCCAAACTGTGGTGTTA+AGG + chr3_1:84505498-84505517 Msa0382030:CDS 50.0%
!! CAAGTTGGTGCTGCTGTAAC+CGG + chr3_1:84505655-84505674 Msa0382030:CDS 50.0%
!! CTGTAACCGGTACTGATGGA+GGG + chr3_1:84505668-84505687 Msa0382030:CDS 50.0%
GGAGCTAATGATGTGTGCAG+TGG + chr3_1:84505793-84505812 Msa0382030:CDS 50.0%
TGTGCAGTGGTGCTCATGTT+TGG + chr3_1:84505806-84505825 Msa0382030:CDS 50.0%
!!! CACCCCTTTTTCAGGGCTAT+AGG - chr3_1:84506161-84506180 None:intergenic 50.0%
CCCCAACTCACTCACACGTT+TGG + chr3_1:84503217-84503236 Msa0382030:five_prime_UTR 55.0%
!! CACCGGTAGAAGCACCAAGA+AGG - chr3_1:84503361-84503380 None:intergenic 55.0%
TTGGTTGTAGGAGGACAGGG+CGG + chr3_1:84503916-84503935 Msa0382030:intron 55.0%
GGAGGGTTGGGTAGTTCAAC+TGG + chr3_1:84503937-84503956 Msa0382030:intron 55.0%
CATGTGTGCTTCCCAGATGG+TGG + chr3_1:84504817-84504836 Msa0382030:intron 55.0%
CGGCAGAAGAATCCACCATC+TGG - chr3_1:84504832-84504851 None:intergenic 55.0%
!! CAGGGTGATTGTGGTGCTGT+GGG + chr3_1:84505427-84505446 Msa0382030:CDS 55.0%
CAGAAAACGCCTCAGGTGCA+TGG - chr3_1:84505579-84505598 None:intergenic 55.0%
! GCTGCTGTAACCGGTACTGA+TGG + chr3_1:84505664-84505683 Msa0382030:CDS 55.0%
!! GCTGTAACCGGTACTGATGG+AGG + chr3_1:84505667-84505686 Msa0382030:CDS 55.0%
AGGAGACCCTCCATCAGTAC+CGG - chr3_1:84505677-84505696 None:intergenic 55.0%
GGTCACCAGTCAGTGTGATG+TGG + chr3_1:84505827-84505846 Msa0382030:CDS 55.0%
TTGTAGGAGGACAGGGCGGA+GGG + chr3_1:84503920-84503939 Msa0382030:intron 60.0%
CCACAGCACCACAATCACCC+TGG - chr3_1:84505429-84505448 None:intergenic 60.0%
! CCAGGGTGATTGTGGTGCTG+TGG + chr3_1:84505426-84505445 Msa0382030:CDS 60.0%
!! GGTGATTGTGGTGCTGTGGG+TGG + chr3_1:84505430-84505449 Msa0382030:CDS 60.0%
GTTGTAGGAGGACAGGGCGG+AGG + chr3_1:84503919-84503938 Msa0382030:intron 65.0%
TAGGGCGAGACATGCGAGCC+CGG - chr3_1:84505389-84505408 None:intergenic 65.0%
AGGAGGACAGGGCGGAGGGT+TGG + chr3_1:84503924-84503943 Msa0382030:intron 70.0%
GGAGGACAGGGCGGAGGGTT+GGG + chr3_1:84503925-84503944 Msa0382030:intron 70.0%
AGGGCGAGACATGCGAGCCC+GGG - chr3_1:84505388-84505407 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 84503126 84506248 84503126 ID=Msa0382030;Name=Msa0382030
chr3_1 mRNA 84503126 84506248 84503126 ID=Msa0382030-mRNA-1;Parent=Msa0382030;Name=Msa0382030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=185|1|1|1|1|1|2|348|227
chr3_1 exon 84503126 84503389 84503126 ID=Msa0382030-mRNA-1:exon:21952;Parent=Msa0382030-mRNA-1
chr3_1 exon 84505296 84506248 84505296 ID=Msa0382030-mRNA-1:exon:21953;Parent=Msa0382030-mRNA-1
chr3_1 five_prime_UTR 84503126 84503310 84503126 ID=Msa0382030-mRNA-1:five_prime_utr;Parent=Msa0382030-mRNA-1
chr3_1 CDS 84503311 84503389 84503311 ID=Msa0382030-mRNA-1:cds;Parent=Msa0382030-mRNA-1
chr3_1 CDS 84505296 84505900 84505296 ID=Msa0382030-mRNA-1:cds;Parent=Msa0382030-mRNA-1
chr3_1 three_prime_UTR 84505901 84506248 84505901 ID=Msa0382030-mRNA-1:three_prime_utr;Parent=Msa0382030-mRNA-1
Gene Sequence

>Msa0382030

ATGCCTAAAACTTTCACACTGTGCTGTCTTTTCTTCATCCTTTCCTTCCTTCTTGGTGCTTCTACCGGTGCTACTCAAATCAAAGTTACTGATAACCCTGCAGACAAGTTAGTAGCTGCTATCAACGAAAACAGAACTGCTCACAAGGATTCATCTTTATTTGACAATCCCGGGCTCGCATGTCTCGCCCTACAGTACATTAAGGCATACCAGGGTGATTGTGGTGCTGTGGGTGGATCTGATGCCAAGAAGCCCCCTGAATCTCAATTTGCTGAAGCTTTTGCCCCAAACTGTGGTGTTAAGGCATCAACTCTTGCTCGTATAACTGGTCGTTTCTTGGGGTGCCAGACTAAGTATGTCCATGCACCTGAGGCGTTTTCTGAAATCCTGATAAGAAACGAGAAGAGCTTAGACATACTCTACAGTAAGAATCACACTCAAGTTGGTGCTGCTGTAACCGGTACTGATGGAGGGTCTCCTTATTTCTGGTGTGTGTTGTTTAGCAATGGCAAACCTAACAGCACATTTGCTTTTGAGGGTGGTGTGGCTAAAGAAACAAAACCAGGTTGCTATAGTGGAGCTAATGATGTGTGCAGTGGTGCTCATGTTTGGTCACCAGTCAGTGTGATGTGGCTATTTGTTGCTTCAGTTTCAATTGCAATGGGGTTTGCTTTTCCATTGTGA
Protein sequence

>Msa0382030

MPKTFTLCCLFFILSFLLGASTGATQIKVTDNPADKLVAAINENRTAHKDSSLFDNPGLACLALQYIKAYQGDCGAVGGSDAKKPPESQFAEAFAPNCGVKASTLARITGRFLGCQTKYVHAPEAFSEILIRNEKSLDILYSKNHTQVGAAVTGTDGGSPYFWCVLFSNGKPNSTFAFEGGVAKETKPGCYSGANDVCSGAHVWSPVSVMWLFVASVSIAMGFAFPL*