AlfalfaGEDB | Msa1224720

M. sativa cultivar zhongmu-4 / Msa1224720

KEGG: K14488
GO: -
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224720	sp\|P32295\|ARG7_VIGRR	53.030	66	30	1	68	132	24	89	8.63e-19	79.0

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224720	G7JS56	97.419	155	4	0	1	155	1	155	8.26e-110	318

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0183730	Msa1224720	0.802970	4.356399e-49	-8.615850e-47
Msa1224720	Msa1266740	0.932503	7.723294e-95	-8.615850e-47
Msa1224720	Msa1280800	0.927743	7.679214e-92	-8.615850e-47
Msa1224720	Msa1363200	0.926498	4.319912e-91	-8.615850e-47
Msa1224720	Msa1447140	0.804742	1.866700e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224720	MtrunA17_Chr4g0070071	97.419	155	4	0	1	155	1	155	1.59e-113	318
Msa1224720	MtrunA17_Chr2g0301531	63.226	155	47	4	1	146	1	154	3.44e-63	191
Msa1224720	MtrunA17_Chr1g0147571	36.184	152	93	2	1	150	36	185	1.02e-24	94.7
Msa1224720	MtrunA17_Chr3g0139171	38.710	124	71	2	11	133	14	133	6.32e-23	89.0
Msa1224720	MtrunA17_Chr1g0178451	52.113	71	32	1	70	138	50	120	8.04e-21	82.4
Msa1224720	MtrunA17_Chr4g0000251	52.941	68	32	0	69	136	70	137	1.25e-20	82.8
Msa1224720	MtrunA17_Chr4g0036841	62.121	66	24	1	68	132	34	99	1.26e-20	81.3
Msa1224720	MtrunA17_Chr4g0036461	44.444	90	43	3	46	132	4	89	1.91e-20	80.5
Msa1224720	MtrunA17_Chr2g0281291	49.383	81	36	2	51	130	12	88	3.13e-20	79.7
Msa1224720	MtrunA17_Chr5g0405301	47.778	90	43	1	43	132	18	103	3.65e-20	80.1
Msa1224720	MtrunA17_Chr4g0009931	47.253	91	47	1	45	134	2	92	3.98e-20	79.7
Msa1224720	MtrunA17_Chr3g0145371	48.750	80	38	1	53	132	32	108	5.16e-20	79.7
Msa1224720	MtrunA17_Chr4g0036491	44.444	90	43	3	46	132	4	89	7.09e-20	79.0
Msa1224720	MtrunA17_Chr4g0036611	45.556	90	42	3	46	132	4	89	1.15e-19	78.2
Msa1224720	MtrunA17_Chr4g0036331	61.017	59	22	1	68	125	22	80	1.27e-19	78.2
Msa1224720	MtrunA17_Chr3g0121721	54.688	64	28	1	68	130	24	87	4.30e-19	77.0
Msa1224720	MtrunA17_Chr5g0396091	53.030	66	30	1	68	132	28	93	4.73e-19	77.0
Msa1224720	MtrunA17_Chr3g0139711	49.383	81	36	2	51	130	12	88	5.37e-19	76.6
Msa1224720	MtrunA17_Chr8g0372061	47.945	73	38	0	61	133	77	149	7.01e-19	77.8
Msa1224720	MtrunA17_Chr4g0036201	48.780	82	37	2	52	132	13	90	8.92e-19	75.9
Msa1224720	MtrunA17_Chr7g0266581	47.826	69	36	0	72	140	53	121	9.37e-19	77.0
Msa1224720	MtrunA17_Chr8g0358971	53.125	64	29	1	68	130	24	87	9.77e-19	75.9
Msa1224720	MtrunA17_Chr3g0121801	57.627	59	24	1	68	125	24	82	1.01e-18	75.9
Msa1224720	MtrunA17_Chr7g0266571	47.826	69	36	0	72	140	53	121	1.08e-18	77.0
Msa1224720	MtrunA17_Chr4g0036571	59.322	59	23	1	68	125	59	117	1.52e-18	76.6
Msa1224720	MtrunA17_Chr4g0036431	43.182	88	47	2	46	132	4	89	1.66e-18	75.5
Msa1224720	MtrunA17_Chr4g0036791	54.688	64	28	1	68	130	26	89	1.99e-18	75.1
Msa1224720	MtrunA17_Chr8g0359011	57.627	59	24	1	68	125	2	60	2.14e-18	74.3
Msa1224720	MtrunA17_Chr4g0036821	54.545	66	29	1	68	132	25	90	3.11e-18	74.7
Msa1224720	MtrunA17_Chr4g0036181	46.591	88	45	2	46	132	61	147	3.50e-18	76.3
Msa1224720	MtrunA17_Chr4g0036551	51.515	66	31	1	68	132	19	84	3.60e-18	74.3
Msa1224720	MtrunA17_Chr4g0036801	53.030	66	30	1	68	132	25	90	4.21e-18	74.3
Msa1224720	MtrunA17_Chr3g0121701	47.500	80	37	2	52	130	12	87	4.62e-18	74.3
Msa1224720	MtrunA17_Chr4g0036811	46.512	86	44	2	46	130	4	88	4.70e-18	74.3
Msa1224720	MtrunA17_Chr3g0121731	47.500	80	37	2	52	130	12	87	4.72e-18	74.3
Msa1224720	MtrunA17_Chr4g0036681	51.515	66	31	1	68	132	24	89	6.32e-18	73.9
Msa1224720	MtrunA17_Chr4g0036531	51.515	66	31	1	68	132	24	89	6.74e-18	73.9
Msa1224720	MtrunA17_Chr8g0359001	59.259	54	21	1	68	120	24	77	7.63e-18	73.2
Msa1224720	MtrunA17_Chr4g0036831	46.512	86	44	2	46	130	4	88	8.60e-18	73.6
Msa1224720	MtrunA17_Chr4g0036751	50.000	74	36	1	61	133	12	85	1.06e-17	73.2
Msa1224720	MtrunA17_Chr4g0036621	55.932	59	25	1	68	125	18	76	1.36e-17	72.8
Msa1224720	MtrunA17_Chr8g0358981	51.562	64	30	1	68	130	24	87	1.63e-17	72.8
Msa1224720	MtrunA17_Chr8g0358991	53.030	66	30	1	68	132	24	89	1.84e-17	72.8
Msa1224720	MtrunA17_Chr4g0036631	50.000	66	32	1	68	132	19	84	1.98e-17	72.4
Msa1224720	MtrunA17_Chr4g0036581	48.485	66	33	1	68	132	24	89	2.46e-17	72.4
Msa1224720	MtrunA17_Chr5g0396081	57.576	66	25	3	61	125	13	76	2.73e-17	72.0
Msa1224720	MtrunA17_Chr4g0036271	54.237	59	26	1	68	125	22	80	3.51e-17	72.0
Msa1224720	MtrunA17_Chr4g0036161	48.529	68	35	0	66	133	33	100	3.67e-17	72.0
Msa1224720	MtrunA17_Chr4g0036691	54.237	59	26	1	68	125	19	77	3.70e-17	71.6
Msa1224720	MtrunA17_Chr3g0121711	51.515	66	31	1	68	132	24	89	4.12e-17	71.6
Msa1224720	MtrunA17_Chr4g0036391	56.897	58	24	1	69	125	20	77	4.21e-17	71.6
Msa1224720	MtrunA17_Chr3g0121741	51.515	66	31	1	68	132	24	89	4.49e-17	71.6
Msa1224720	MtrunA17_Chr4g0036221	54.688	64	27	2	68	130	25	87	4.74e-17	71.6
Msa1224720	MtrunA17_Chr4g0036651	40.909	88	49	2	46	132	4	89	5.69e-17	71.6
Msa1224720	MtrunA17_Chr4g0036601	51.562	64	30	1	68	130	20	83	6.24e-17	71.2
Msa1224720	MtrunA17_Chr3g0121761	51.515	66	31	1	68	132	24	89	7.28e-17	71.2
Msa1224720	MtrunA17_Chr7g0214201	46.970	66	35	0	69	134	67	132	7.30e-17	73.2
Msa1224720	MtrunA17_Chr4g0036471	54.237	59	26	1	68	125	20	78	8.43e-17	70.9
Msa1224720	MtrunA17_Chr4g0036641	54.237	59	26	1	68	125	20	78	8.43e-17	70.9
Msa1224720	MtrunA17_Chr4g0036671	54.237	59	26	1	68	125	20	78	8.80e-17	70.9
Msa1224720	MtrunA17_Chr3g0121781	51.515	66	31	1	68	132	24	89	9.03e-17	70.9
Msa1224720	MtrunA17_Chr4g0036561	51.515	66	31	1	68	132	19	84	9.64e-17	70.9
Msa1224720	MtrunA17_Chr4g0036861	54.839	62	27	1	68	128	56	117	1.06e-16	71.6
Msa1224720	MtrunA17_Chr4g0036241	51.562	64	30	1	68	130	25	88	1.08e-16	70.9
Msa1224720	MtrunA17_Chr4g0036211	44.444	90	48	2	46	134	142	230	1.20e-16	74.3
Msa1224720	MtrunA17_Chr4g0036211	46.667	75	38	2	46	119	4	77	2.32e-14	68.2
Msa1224720	MtrunA17_Chr4g0036191	53.125	64	27	2	68	130	25	86	1.28e-16	70.5
Msa1224720	MtrunA17_Chr4g0036141	44.444	72	40	0	67	138	35	106	1.34e-16	71.6
Msa1224720	MtrunA17_Chr4g0036441	52.542	59	27	1	68	125	19	77	1.47e-16	70.1
Msa1224720	MtrunA17_Chr1g0179511	43.836	73	41	0	57	129	7	79	1.53e-16	71.2
Msa1224720	MtrunA17_Chr3g0139731	53.125	64	29	1	68	130	63	126	1.54e-16	71.6
Msa1224720	MtrunA17_Chr4g0036411	48.148	81	35	3	46	125	4	78	1.64e-16	70.1
Msa1224720	MtrunA17_Chr8g0346181	41.026	78	43	2	69	143	27	104	2.62e-16	70.1
Msa1224720	MtrunA17_Chr4g0036401	46.970	66	34	1	68	132	24	89	3.01e-16	69.7
Msa1224720	MtrunA17_Chr4g0036761	54.237	59	26	1	68	125	22	80	3.02e-16	69.7
Msa1224720	MtrunA17_Chr4g0036501	54.237	59	26	1	68	125	20	78	3.38e-16	69.3
Msa1224720	MtrunA17_Chr4g0036521	51.471	68	31	2	68	133	31	98	3.80e-16	69.7
Msa1224720	MtrunA17_Chr4g0036661	55.172	58	25	1	69	125	20	77	4.00e-16	68.9
Msa1224720	MtrunA17_Chr3g0139701	54.237	59	26	1	68	125	82	140	5.19e-16	70.5
Msa1224720	MtrunA17_Chr3g0145411	53.425	73	27	3	68	138	33	100	5.52e-16	69.3
Msa1224720	MtrunA17_Chr3g0121811	50.000	70	30	2	52	120	13	78	6.15e-16	68.9
Msa1224720	MtrunA17_Chr3g0145401	51.562	64	30	1	68	130	44	107	8.91e-16	68.9
Msa1224720	MtrunA17_Chr1g0156341	46.970	66	35	0	64	129	32	97	9.08e-16	69.7
Msa1224720	MtrunA17_Chr4g0036541	54.237	59	26	1	68	125	19	77	1.03e-15	68.2
Msa1224720	MtrunA17_Chr3g0127561	39.744	78	45	1	68	143	32	109	1.08e-15	68.9
Msa1224720	MtrunA17_Chr4g0036231	50.000	72	31	2	50	120	19	86	1.08e-15	68.2
Msa1224720	MtrunA17_Chr4g0036421	44.444	81	37	2	46	125	4	77	1.21e-15	67.8
Msa1224720	MtrunA17_Chr4g0000241	50.794	63	29	1	69	129	62	124	1.84e-15	69.3
Msa1224720	MtrunA17_Chr3g0142421	46.970	66	35	0	68	133	41	106	1.91e-15	67.8
Msa1224720	MtrunA17_Chr7g0276101	40.789	76	42	2	69	141	23	98	6.05e-15	66.6
Msa1224720	MtrunA17_Chr5g0396071	46.970	66	34	1	68	132	20	85	6.33e-15	66.2
Msa1224720	MtrunA17_Chr4g0036341	44.304	79	42	2	61	138	13	90	6.52e-15	66.2
Msa1224720	MtrunA17_Chr8g0358871	38.750	80	46	1	68	144	34	113	7.76e-15	66.6
Msa1224720	MtrunA17_Chr4g0011821	51.667	60	26	1	69	125	79	138	1.90e-14	66.6
Msa1224720	MtrunA17_Chr4g0036891	48.529	68	33	2	68	133	26	93	2.43e-14	64.7
Msa1224720	MtrunA17_Chr1g0199531	43.590	78	44	0	52	129	33	110	3.56e-14	64.7
Msa1224720	MtrunA17_Chr7g0264401	46.479	71	38	0	66	136	37	107	7.03e-14	63.9
Msa1224720	MtrunA17_Chr4g0036711	46.269	67	35	1	69	134	15	81	7.58e-14	63.2
Msa1224720	MtrunA17_Chr8g0386121	44.615	65	36	0	70	134	47	111	1.69e-13	63.2
Msa1224720	MtrunA17_Chr3g0121751	50.820	61	25	2	51	111	70	125	3.46e-13	63.5
Msa1224720	MtrunA17_Chr1g0181011	39.286	84	48	1	53	136	76	156	3.75e-13	63.5
Msa1224720	MtrunA17_Chr6g0481791	40.299	67	39	1	68	133	33	99	4.28e-13	61.6
Msa1224720	MtrunA17_Chr7g0230411	40.260	77	41	2	61	134	69	143	4.55e-13	62.8
Msa1224720	MtrunA17_Chr8g0386131	42.029	69	40	0	70	138	50	118	4.99e-13	62.8
Msa1224720	MtrunA17_Chr3g0131991	38.235	68	42	0	69	136	53	120	5.44e-13	62.8
Msa1224720	MtrunA17_Chr8g0386111	42.029	69	40	0	70	138	46	114	6.14e-13	62.4
Msa1224720	MtrunA17_Chr8g0389151	40.506	79	41	1	48	126	25	97	1.69e-12	61.2
Msa1224720	MtrunA17_Chr2g0311341	36.986	73	44	1	61	133	52	122	2.96e-12	60.5
Msa1224720	MtrunA17_Chr8g0389141	33.654	104	61	2	43	146	27	122	1.14e-11	59.3
Msa1224720	MtrunA17_Chr7g0264551	33.696	92	56	2	32	121	18	106	1.56e-11	58.2
Msa1224720	MtrunA17_Chr4g0036701	51.852	54	25	1	62	115	26	78	1.59e-11	57.4
Msa1224720	MtrunA17_Chr2g0327281	37.079	89	49	2	37	121	16	101	1.98e-11	58.2
Msa1224720	MtrunA17_Chr4g0028491	38.961	77	40	2	68	142	37	108	2.87e-11	57.4
Msa1224720	MtrunA17_Chr8g0385011	42.466	73	29	1	69	128	3	75	3.35e-11	57.0
Msa1224720	MtrunA17_Chr3g0130911	44.118	68	30	1	69	128	9	76	4.09e-11	56.6
Msa1224720	MtrunA17_Chr4g0028481	46.970	66	31	1	68	129	37	102	4.30e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224720	AT5G20810.1	58.537	164	56	4	1	152	1	164	7.43e-59	180
Msa1224720	AT5G20810.2	60.645	155	49	4	1	143	1	155	5.33e-57	176
Msa1224720	AT3G43120.1	56.790	162	58	5	1	152	1	160	3.17e-55	171
Msa1224720	AT4G31320.1	49.425	87	38	1	69	155	80	160	6.89e-21	84.7
Msa1224720	AT2G24400.1	58.462	65	27	0	69	133	64	128	8.64e-21	84.0
Msa1224720	AT2G21220.1	45.000	100	49	2	32	130	7	101	1.72e-19	78.6
Msa1224720	AT4G34800.1	53.947	76	31	2	58	129	10	85	2.91e-19	77.8
Msa1224720	AT2G18010.1	53.846	65	30	0	66	130	45	109	8.18e-19	77.0
Msa1224720	AT3G61900.1	39.815	108	61	2	45	148	6	113	1.74e-18	77.0
Msa1224720	AT3G51200.1	45.161	93	42	2	37	129	17	100	4.32e-18	75.1
Msa1224720	AT1G19830.1	38.889	108	62	1	29	132	4	111	4.33e-18	75.5
Msa1224720	AT2G46690.1	49.275	69	32	1	68	133	23	91	5.57e-18	75.1
Msa1224720	AT4G38840.1	58.462	65	25	2	68	130	32	96	1.46e-17	73.6
Msa1224720	AT3G20220.1	46.575	73	38	1	58	129	36	108	1.80e-17	73.9
Msa1224720	AT4G34790.1	50.685	73	30	3	69	138	39	108	1.92e-17	73.6
Msa1224720	AT5G66260.1	50.000	64	32	0	69	132	34	97	2.15e-17	73.2
Msa1224720	AT1G75580.1	40.659	91	54	0	43	133	18	108	3.29e-17	72.8
Msa1224720	AT2G21210.1	51.429	70	31	2	69	135	28	97	4.36e-17	72.4
Msa1224720	AT4G34760.1	50.769	65	32	0	66	130	40	104	7.19e-17	72.0
Msa1224720	AT4G00880.1	50.685	73	32	2	69	137	28	100	1.55e-16	71.6
Msa1224720	AT4G38860.1	53.030	66	30	1	66	130	37	102	2.16e-16	70.9
Msa1224720	AT2G21210.2	56.667	60	23	2	69	125	95	154	2.36e-16	72.0
Msa1224720	AT4G34810.1	50.746	67	28	2	69	130	48	114	3.25e-16	70.5
Msa1224720	AT4G34770.1	43.678	87	46	2	52	135	18	104	4.41e-16	69.7
Msa1224720	AT4G38825.1	50.704	71	28	3	70	138	24	89	5.26e-16	69.3
Msa1224720	AT4G36110.1	49.206	63	32	0	68	130	39	101	1.06e-15	68.9
Msa1224720	AT2G37030.1	48.485	66	34	0	66	131	46	111	1.59e-15	68.9
Msa1224720	AT5G10990.1	50.000	62	31	0	65	126	37	98	1.84e-15	69.3
Msa1224720	AT3G03820.1	50.704	71	31	2	68	134	26	96	2.83e-15	67.4
Msa1224720	AT1G75590.1	48.387	62	32	0	65	126	38	99	4.05e-15	68.6
Msa1224720	AT5G18050.1	50.746	67	31	2	61	125	16	82	5.43e-15	66.6
Msa1224720	AT1G56150.1	35.714	84	54	0	49	132	18	101	8.30e-15	66.6
Msa1224720	AT5G18030.1	54.237	59	25	2	69	125	22	80	1.31e-14	65.5
Msa1224720	AT3G60690.1	44.444	72	37	1	66	134	85	156	1.45e-14	67.8
Msa1224720	AT2G16580.1	47.059	68	36	0	66	133	41	108	1.71e-14	65.9
Msa1224720	AT5G18010.1	50.746	67	31	2	61	125	16	82	2.19e-14	65.1
Msa1224720	AT5G18020.1	49.254	67	32	2	61	125	16	82	2.22e-14	65.1
Msa1224720	AT2G36210.1	38.710	93	52	2	49	140	38	126	3.68e-14	65.9
Msa1224720	AT3G03850.1	48.649	74	32	3	64	134	23	93	3.78e-14	64.7
Msa1224720	AT3G12830.1	35.366	82	53	0	52	133	33	114	6.87e-14	65.1
Msa1224720	AT5G18060.1	53.448	58	25	2	70	125	25	82	7.35e-14	63.5
Msa1224720	AT1G19840.1	48.387	62	32	0	65	126	37	98	1.05e-13	65.1
Msa1224720	AT4G34780.1	53.226	62	25	2	68	125	27	88	1.19e-13	63.5
Msa1224720	AT5G53590.1	43.590	78	38	2	68	139	45	122	1.29e-13	64.3
Msa1224720	AT3G53250.1	43.939	66	34	1	66	131	49	111	2.49e-13	63.2
Msa1224720	AT3G03840.1	54.237	59	24	2	70	125	28	86	3.52e-13	62.0
Msa1224720	AT4G34750.2	35.897	78	47	1	68	145	36	110	6.61e-13	62.8
Msa1224720	AT4G34750.1	35.897	78	47	1	68	145	36	110	6.61e-13	62.8
Msa1224720	AT4G38850.1	46.667	60	28	2	70	125	22	81	1.01e-12	60.8
Msa1224720	AT3G53250.2	43.939	66	34	1	66	131	80	142	1.57e-12	62.0
Msa1224720	AT4G22620.1	44.118	68	34	2	61	125	72	138	2.00e-12	61.6
Msa1224720	AT4G09530.1	38.028	71	40	2	70	136	29	99	2.97e-12	60.1
Msa1224720	AT4G13790.1	49.123	57	26	2	68	121	24	80	3.11e-12	59.7
Msa1224720	AT1G16510.1	36.923	65	41	0	69	133	54	118	5.86e-12	60.1
Msa1224720	AT1G20470.1	43.284	67	37	1	71	137	25	90	9.22e-12	59.7
Msa1224720	AT5G50760.1	41.270	63	37	0	72	134	58	120	1.77e-11	59.7
Msa1224720	AT2G28085.1	31.579	114	66	4	28	130	8	120	1.80e-11	58.5
Msa1224720	AT4G12410.1	42.857	63	33	1	69	128	76	138	2.36e-11	58.9

Find 40 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAGCTGAGAAGGTATTTAA+AGG	0.276472	8_1:+89815171	None:intergenic
GGAACATCGTGCGGTGCTTT+AGG	0.291352	8_1:+89815355	None:intergenic
GTTATCTTCTATTCAATATC+AGG	0.295590	8_1:-89816020	Msa1224720:intron
GAGGGCAATATTGATCATTC+TGG	0.379305	8_1:-89815466	Msa1224720:CDS
GAAGATAACATTAGATATGA+AGG	0.399265	8_1:+89816034	None:intergenic
TGGCAGAGCGTTTGTTAAGC+TGG	0.411349	8_1:+89816076	None:intergenic
TCTATGTTGGTCCTGAGCTT+AGG	0.426326	8_1:-89815315	Msa1224720:CDS
TAAATACCTTCTCAGCTGTA+TGG	0.429520	8_1:-89815167	Msa1224720:CDS
ATAGACAGCTAAGTATCCTT+TGG	0.430579	8_1:+89815333	None:intergenic
ATCTCTGTATTTAGTGTGAC+AGG	0.434760	8_1:-89815627	Msa1224720:intron
AAATCTGCTAAACTTGATCC+TGG	0.454410	8_1:-89815600	Msa1224720:five_prime_UTR
TGAAAGAAATATTTCAAAAG+TGG	0.457045	8_1:-89815504	Msa1224720:CDS
TAGACAGCTAAGTATCCTTT+GGG	0.468981	8_1:+89815334	None:intergenic
TGGCAAAAGCTTATCATCAA+TGG	0.473791	8_1:-89815560	Msa1224720:exon
TTTCACCTATGATTAACAAA+AGG	0.486292	8_1:-89815426	Msa1224720:CDS
AGAGGTGCAAATCATAGTGC+TGG	0.508610	8_1:-89814958	Msa1224720:three_prime_UTR
GAGCGTTTGTTAAGCTGGAA+TGG	0.513920	8_1:+89816081	None:intergenic
AACTAGTATGATACTTAAAG+AGG	0.531529	8_1:-89814976	Msa1224720:exon
TTATCATCAATGGACGATGC+TGG	0.539963	8_1:-89815550	Msa1224720:exon
GAGTGGCTAAGGTAGCTTGT+GGG	0.551541	8_1:+89815280	None:intergenic
GGATACTTAGCTGTCTATGT+TGG	0.555421	8_1:-89815328	Msa1224720:CDS
ACAAGAGTTAAAAGTATCCC+AGG	0.556086	8_1:+89815582	None:intergenic
TGAAGGGATGTCAATGAGAT+CGG	0.558302	8_1:+89816051	None:intergenic
TATTCTCCATACAGCTGAGA+AGG	0.559878	8_1:+89815161	None:intergenic
AATATTTCAAAAGTGGCAAG+TGG	0.559917	8_1:-89815497	Msa1224720:CDS
GATCATTCTGGTGTGAATCA+TGG	0.562412	8_1:-89815454	Msa1224720:CDS
ATGATGAATCTCCTAAGCTC+AGG	0.564919	8_1:+89815304	None:intergenic
GTGGCAAGTGGGTTATAAAG+AGG	0.566612	8_1:-89815485	Msa1224720:CDS
AAGATAACATTAGATATGAA+GGG	0.591038	8_1:+89816035	None:intergenic
AATCTGCTAAACTTGATCCT+GGG	0.596689	8_1:-89815599	Msa1224720:five_prime_UTR
TGGCAAGTGGGTTATAAAGA+GGG	0.604345	8_1:-89815484	Msa1224720:CDS
GAAATTGCAGCTGGAAACAC+AGG	0.604667	8_1:-89815018	Msa1224720:intron
TTAAAGGTTTCAATTTCACA+TGG	0.605541	8_1:+89815187	None:intergenic
ATATTTCAAAAGTGGCAAGT+GGG	0.643378	8_1:-89815496	Msa1224720:CDS
TGAGTGGCTAAGGTAGCTTG+TGG	0.646992	8_1:+89815279	None:intergenic
GGATGTCAATGAGATCGGAA+TGG	0.652039	8_1:+89816056	None:intergenic
TATCCTTTGGGAACATCGTG+CGG	0.653439	8_1:+89815346	None:intergenic
GCACCGCACGATGTTCCCAA+AGG	0.669257	8_1:-89815349	Msa1224720:CDS
AGCTGGAAACACAGGAACTG+TGG	0.675127	8_1:-89815010	Msa1224720:CDS
CATTCTGGTGTGAATCATGG+AGG	0.741290	8_1:-89815451	Msa1224720:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TACTTTATATAATTTCATAT+AGG	-	chr8_1:89815955-89815974	Msa1224720:intron	10.0%
!!	TAATTAGTTAAAAAAAGAAA+AGG	+	chr8_1:89816005-89816024	None:intergenic	10.0%
!!	TACTTTATATAATTTCATAT+AGG	-	chr8_1:89815955-89815974	Msa1224720:intron	10.0%
!!	TAATTAGTTAAAAAAAGAAA+AGG	+	chr8_1:89816005-89816024	None:intergenic	10.0%
!!!	TTTAAAATTTCTGTAATCAT+GGG	+	chr8_1:89815201-89815220	None:intergenic	15.0%
!!!	ATTTAAAATTTCTGTAATCA+TGG	+	chr8_1:89815202-89815221	None:intergenic	15.0%
!!!	TTTAAAATTTCTGTAATCAT+GGG	+	chr8_1:89815201-89815220	None:intergenic	15.0%
!!!	ATTTAAAATTTCTGTAATCA+TGG	+	chr8_1:89815202-89815221	None:intergenic	15.0%
!!	AAGATAACATTAGATATGAA+GGG	+	chr8_1:89815024-89815043	None:intergenic	20.0%
!!	TAAATACAGAGATGTTATAA+AGG	+	chr8_1:89815422-89815441	None:intergenic	20.0%
!!	TGAAAGAAATATTTCAAAAG+TGG	-	chr8_1:89815552-89815571	Msa1224720:exon	20.0%
!!!	TTTAACCTTTTGTTAATCAT+AGG	+	chr8_1:89815638-89815657	None:intergenic	20.0%
!!	TAAAGTAATTAACTTACAAG+AGG	+	chr8_1:89815942-89815961	None:intergenic	20.0%
!!	TTAACTAATTATTCGACTAT+TGG	-	chr8_1:89816014-89816033	Msa1224720:intron	20.0%
!!!	AATGATGTTTCTCAATTTTA+TGG	+	chr8_1:89814936-89814955	None:intergenic	20.0%
!!	AAGATAACATTAGATATGAA+GGG	+	chr8_1:89815024-89815043	None:intergenic	20.0%
!!	TAAATACAGAGATGTTATAA+AGG	+	chr8_1:89815422-89815441	None:intergenic	20.0%
!!	TGAAAGAAATATTTCAAAAG+TGG	-	chr8_1:89815552-89815571	Msa1224720:exon	20.0%
!!!	TTTAACCTTTTGTTAATCAT+AGG	+	chr8_1:89815638-89815657	None:intergenic	20.0%
!!	TAAAGTAATTAACTTACAAG+AGG	+	chr8_1:89815942-89815961	None:intergenic	20.0%
!!	TTAACTAATTATTCGACTAT+TGG	-	chr8_1:89816014-89816033	Msa1224720:intron	20.0%
!	GAAGATAACATTAGATATGA+AGG	+	chr8_1:89815025-89815044	None:intergenic	25.0%
!	GTTATCTTCTATTCAATATC+AGG	-	chr8_1:89815036-89815055	Msa1224720:intron	25.0%
!	CATTGTAGTGTTAACAAAAA+AGG	+	chr8_1:89815095-89815114	None:intergenic	25.0%
!!	GTTTTCTACTATTCTATGAA+TGG	-	chr8_1:89815151-89815170	Msa1224720:CDS	25.0%
!	TTTCACCTATGATTAACAAA+AGG	-	chr8_1:89815630-89815649	Msa1224720:intron	25.0%
!	TTAAAGGTTTCAATTTCACA+TGG	+	chr8_1:89815872-89815891	None:intergenic	25.0%
!	AACTAGTATGATACTTAAAG+AGG	-	chr8_1:89816080-89816099	Msa1224720:five_prime_UTR	25.0%
!	GAAGATAACATTAGATATGA+AGG	+	chr8_1:89815025-89815044	None:intergenic	25.0%
!	GTTATCTTCTATTCAATATC+AGG	-	chr8_1:89815036-89815055	Msa1224720:intron	25.0%
!	CATTGTAGTGTTAACAAAAA+AGG	+	chr8_1:89815095-89815114	None:intergenic	25.0%
!!	GTTTTCTACTATTCTATGAA+TGG	-	chr8_1:89815151-89815170	Msa1224720:CDS	25.0%
!	TTTCACCTATGATTAACAAA+AGG	-	chr8_1:89815630-89815649	Msa1224720:intron	25.0%
!	TTAAAGGTTTCAATTTCACA+TGG	+	chr8_1:89815872-89815891	None:intergenic	25.0%
!	AACTAGTATGATACTTAAAG+AGG	-	chr8_1:89816080-89816099	Msa1224720:five_prime_UTR	25.0%
!	CAGGTAACTGCTAAATTTTA+TGG	+	chr8_1:89815261-89815280	None:intergenic	30.0%
	AATATTTCAAAAGTGGCAAG+TGG	-	chr8_1:89815559-89815578	Msa1224720:exon	30.0%
	ATATTTCAAAAGTGGCAAGT+GGG	-	chr8_1:89815560-89815579	Msa1224720:exon	30.0%
!!	ACAACATTTTGAAAAGTGAG+TGG	+	chr8_1:89815796-89815815	None:intergenic	30.0%
!!	CTCACTTTTCAAAATGTTGT+TGG	-	chr8_1:89815796-89815815	Msa1224720:intron	30.0%
!!!	GAATTTGGTTTTAATCAGTG+TGG	-	chr8_1:89815833-89815852	Msa1224720:intron	30.0%
!	CAGGTAACTGCTAAATTTTA+TGG	+	chr8_1:89815261-89815280	None:intergenic	30.0%
	AATATTTCAAAAGTGGCAAG+TGG	-	chr8_1:89815559-89815578	Msa1224720:exon	30.0%
	ATATTTCAAAAGTGGCAAGT+GGG	-	chr8_1:89815560-89815579	Msa1224720:exon	30.0%
!!	ACAACATTTTGAAAAGTGAG+TGG	+	chr8_1:89815796-89815815	None:intergenic	30.0%
!!	CTCACTTTTCAAAATGTTGT+TGG	-	chr8_1:89815796-89815815	Msa1224720:intron	30.0%
!!!	GAATTTGGTTTTAATCAGTG+TGG	-	chr8_1:89815833-89815852	Msa1224720:intron	30.0%
	GGATCTATGATCATCTATCT+TGG	-	chr8_1:89815172-89815191	Msa1224720:CDS	35.0%
	CTTTAGATTGAAGACGTGTT+AGG	+	chr8_1:89815349-89815368	None:intergenic	35.0%
	ATCTCTGTATTTAGTGTGAC+AGG	-	chr8_1:89815429-89815448	Msa1224720:CDS	35.0%
	AAATCTGCTAAACTTGATCC+TGG	-	chr8_1:89815456-89815475	Msa1224720:CDS	35.0%
	AATCTGCTAAACTTGATCCT+GGG	-	chr8_1:89815457-89815476	Msa1224720:CDS	35.0%
!!	ACAAGAGTTAAAAGTATCCC+AGG	+	chr8_1:89815477-89815496	None:intergenic	35.0%
!!	TGGGATACTTTTAACTCTTG+TGG	-	chr8_1:89815476-89815495	Msa1224720:CDS	35.0%
	TGGCAAAAGCTTATCATCAA+TGG	-	chr8_1:89815496-89815515	Msa1224720:CDS	35.0%
	TAGACAGCTAAGTATCCTTT+GGG	+	chr8_1:89815725-89815744	None:intergenic	35.0%
	ATAGACAGCTAAGTATCCTT+TGG	+	chr8_1:89815726-89815745	None:intergenic	35.0%
!!	TTTTGAAAAGTGAGTGGCTA+AGG	+	chr8_1:89815790-89815809	None:intergenic	35.0%
	GAAAAAGCTGCTGATGAATT+TGG	-	chr8_1:89815818-89815837	Msa1224720:intron	35.0%
!!!	TTTGGTTTTAATCAGTGTGG+TGG	-	chr8_1:89815836-89815855	Msa1224720:intron	35.0%
!!	ACAGCTGAGAAGGTATTTAA+AGG	+	chr8_1:89815888-89815907	None:intergenic	35.0%
	TAAATACCTTCTCAGCTGTA+TGG	-	chr8_1:89815889-89815908	Msa1224720:intron	35.0%
	GGATCTATGATCATCTATCT+TGG	-	chr8_1:89815172-89815191	Msa1224720:CDS	35.0%
	CTTTAGATTGAAGACGTGTT+AGG	+	chr8_1:89815349-89815368	None:intergenic	35.0%
	ATCTCTGTATTTAGTGTGAC+AGG	-	chr8_1:89815429-89815448	Msa1224720:CDS	35.0%
	AAATCTGCTAAACTTGATCC+TGG	-	chr8_1:89815456-89815475	Msa1224720:CDS	35.0%
	AATCTGCTAAACTTGATCCT+GGG	-	chr8_1:89815457-89815476	Msa1224720:CDS	35.0%
!!	ACAAGAGTTAAAAGTATCCC+AGG	+	chr8_1:89815477-89815496	None:intergenic	35.0%
!!	TGGGATACTTTTAACTCTTG+TGG	-	chr8_1:89815476-89815495	Msa1224720:CDS	35.0%
	TGGCAAAAGCTTATCATCAA+TGG	-	chr8_1:89815496-89815515	Msa1224720:CDS	35.0%
	TAGACAGCTAAGTATCCTTT+GGG	+	chr8_1:89815725-89815744	None:intergenic	35.0%
	ATAGACAGCTAAGTATCCTT+TGG	+	chr8_1:89815726-89815745	None:intergenic	35.0%
!!	TTTTGAAAAGTGAGTGGCTA+AGG	+	chr8_1:89815790-89815809	None:intergenic	35.0%
	GAAAAAGCTGCTGATGAATT+TGG	-	chr8_1:89815818-89815837	Msa1224720:intron	35.0%
!!!	TTTGGTTTTAATCAGTGTGG+TGG	-	chr8_1:89815836-89815855	Msa1224720:intron	35.0%
!!	ACAGCTGAGAAGGTATTTAA+AGG	+	chr8_1:89815888-89815907	None:intergenic	35.0%
	TAAATACCTTCTCAGCTGTA+TGG	-	chr8_1:89815889-89815908	Msa1224720:intron	35.0%
	TGAAGGGATGTCAATGAGAT+CGG	+	chr8_1:89815008-89815027	None:intergenic	40.0%
	TTATCATCAATGGACGATGC+TGG	-	chr8_1:89815506-89815525	Msa1224720:CDS	40.0%
!!	TGGCAAGTGGGTTATAAAGA+GGG	-	chr8_1:89815572-89815591	Msa1224720:five_prime_UTR	40.0%
	GAGGGCAATATTGATCATTC+TGG	-	chr8_1:89815590-89815609	Msa1224720:five_prime_UTR	40.0%
	GATCATTCTGGTGTGAATCA+TGG	-	chr8_1:89815602-89815621	Msa1224720:five_prime_UTR	40.0%
	GGATACTTAGCTGTCTATGT+TGG	-	chr8_1:89815728-89815747	Msa1224720:intron	40.0%
	ATGATGAATCTCCTAAGCTC+AGG	+	chr8_1:89815755-89815774	None:intergenic	40.0%
	TATTCTCCATACAGCTGAGA+AGG	+	chr8_1:89815898-89815917	None:intergenic	40.0%
	ACTATTGGTGAAATTGCAGC+TGG	-	chr8_1:89816029-89816048	Msa1224720:five_prime_UTR	40.0%
	TGAAGGGATGTCAATGAGAT+CGG	+	chr8_1:89815008-89815027	None:intergenic	40.0%
	TTATCATCAATGGACGATGC+TGG	-	chr8_1:89815506-89815525	Msa1224720:CDS	40.0%
!!	TGGCAAGTGGGTTATAAAGA+GGG	-	chr8_1:89815572-89815591	Msa1224720:five_prime_UTR	40.0%
	GAGGGCAATATTGATCATTC+TGG	-	chr8_1:89815590-89815609	Msa1224720:five_prime_UTR	40.0%
	GATCATTCTGGTGTGAATCA+TGG	-	chr8_1:89815602-89815621	Msa1224720:five_prime_UTR	40.0%
	GGATACTTAGCTGTCTATGT+TGG	-	chr8_1:89815728-89815747	Msa1224720:intron	40.0%
	ATGATGAATCTCCTAAGCTC+AGG	+	chr8_1:89815755-89815774	None:intergenic	40.0%
	TATTCTCCATACAGCTGAGA+AGG	+	chr8_1:89815898-89815917	None:intergenic	40.0%
	ACTATTGGTGAAATTGCAGC+TGG	-	chr8_1:89816029-89816048	Msa1224720:five_prime_UTR	40.0%
!	GAGCGTTTGTTAAGCTGGAA+TGG	+	chr8_1:89814978-89814997	None:intergenic	45.0%
	GGATGTCAATGAGATCGGAA+TGG	+	chr8_1:89815003-89815022	None:intergenic	45.0%
	TGTTAACTAGTGTGCAGTGC+AGG	+	chr8_1:89815280-89815299	None:intergenic	45.0%
!!	GTGGCAAGTGGGTTATAAAG+AGG	-	chr8_1:89815571-89815590	Msa1224720:five_prime_UTR	45.0%
	CATTCTGGTGTGAATCATGG+AGG	-	chr8_1:89815605-89815624	Msa1224720:five_prime_UTR	45.0%
!	TATCCTTTGGGAACATCGTG+CGG	+	chr8_1:89815713-89815732	None:intergenic	45.0%
	TCTATGTTGGTCCTGAGCTT+AGG	-	chr8_1:89815741-89815760	Msa1224720:intron	45.0%
	GAAATTGCAGCTGGAAACAC+AGG	-	chr8_1:89816038-89816057	Msa1224720:five_prime_UTR	45.0%
!	AGAGGTGCAAATCATAGTGC+TGG	-	chr8_1:89816098-89816117	Msa1224720:five_prime_UTR	45.0%
!	GAGCGTTTGTTAAGCTGGAA+TGG	+	chr8_1:89814978-89814997	None:intergenic	45.0%
	GGATGTCAATGAGATCGGAA+TGG	+	chr8_1:89815003-89815022	None:intergenic	45.0%
	TGTTAACTAGTGTGCAGTGC+AGG	+	chr8_1:89815280-89815299	None:intergenic	45.0%
!!	GTGGCAAGTGGGTTATAAAG+AGG	-	chr8_1:89815571-89815590	Msa1224720:five_prime_UTR	45.0%
	CATTCTGGTGTGAATCATGG+AGG	-	chr8_1:89815605-89815624	Msa1224720:five_prime_UTR	45.0%
!	TATCCTTTGGGAACATCGTG+CGG	+	chr8_1:89815713-89815732	None:intergenic	45.0%
	TCTATGTTGGTCCTGAGCTT+AGG	-	chr8_1:89815741-89815760	Msa1224720:intron	45.0%
	GAAATTGCAGCTGGAAACAC+AGG	-	chr8_1:89816038-89816057	Msa1224720:five_prime_UTR	45.0%
!	AGAGGTGCAAATCATAGTGC+TGG	-	chr8_1:89816098-89816117	Msa1224720:five_prime_UTR	45.0%
!	TGGCAGAGCGTTTGTTAAGC+TGG	+	chr8_1:89814983-89815002	None:intergenic	50.0%
!	GAGTGGCTAAGGTAGCTTGT+GGG	+	chr8_1:89815779-89815798	None:intergenic	50.0%
	TGAGTGGCTAAGGTAGCTTG+TGG	+	chr8_1:89815780-89815799	None:intergenic	50.0%
	AGCTGGAAACACAGGAACTG+TGG	-	chr8_1:89816046-89816065	Msa1224720:five_prime_UTR	50.0%
!	TGGCAGAGCGTTTGTTAAGC+TGG	+	chr8_1:89814983-89815002	None:intergenic	50.0%
!	GAGTGGCTAAGGTAGCTTGT+GGG	+	chr8_1:89815779-89815798	None:intergenic	50.0%
	TGAGTGGCTAAGGTAGCTTG+TGG	+	chr8_1:89815780-89815799	None:intergenic	50.0%
	AGCTGGAAACACAGGAACTG+TGG	-	chr8_1:89816046-89816065	Msa1224720:five_prime_UTR	50.0%
	GGAACATCGTGCGGTGCTTT+AGG	+	chr8_1:89815704-89815723	None:intergenic	55.0%
	GGAACATCGTGCGGTGCTTT+AGG	+	chr8_1:89815704-89815723	None:intergenic	55.0%
	GCACCGCACGATGTTCCCAA+AGG	-	chr8_1:89815707-89815726	Msa1224720:intron	60.0%
	GCACCGCACGATGTTCCCAA+AGG	-	chr8_1:89815707-89815726	Msa1224720:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	89814935	89816143	89814935	ID=Msa1224720;Name=Msa1224720
chr8_1	mRNA	89814935	89816143	89814935	ID=Msa1224720-mRNA-1;Parent=Msa1224720;Name=Msa1224720-mRNA-1;_AED=0.01;_eAED=0.01;_QI=195\|1\|1\|1\|0.5\|0.33\|3\|58\|155
chr8_1	exon	89814935	89815030	89814935	ID=Msa1224720-mRNA-1:exon:32619;Parent=Msa1224720-mRNA-1
chr8_1	exon	89815134	89815635	89815134	ID=Msa1224720-mRNA-1:exon:32618;Parent=Msa1224720-mRNA-1
chr8_1	exon	89816021	89816143	89816021	ID=Msa1224720-mRNA-1:exon:32617;Parent=Msa1224720-mRNA-1
chr8_1	five_prime_UTR	89816021	89816143	89816021	ID=Msa1224720-mRNA-1:five_prime_utr;Parent=Msa1224720-mRNA-1
chr8_1	five_prime_UTR	89815564	89815635	89815564	ID=Msa1224720-mRNA-1:five_prime_utr;Parent=Msa1224720-mRNA-1
chr8_1	CDS	89815134	89815563	89815134	ID=Msa1224720-mRNA-1:cds;Parent=Msa1224720-mRNA-1
chr8_1	CDS	89814993	89815030	89814993	ID=Msa1224720-mRNA-1:cds;Parent=Msa1224720-mRNA-1
chr8_1	three_prime_UTR	89814935	89814992	89814935	ID=Msa1224720-mRNA-1:three_prime_utr;Parent=Msa1224720-mRNA-1

Gene Sequence

>Msa1224720

ATGGACGATGCTGGAGTCTCGAAATTAACTGCTAAATTGAAAGAAATATTTCAAAAGTGGCAAGTGGGTTATAAAGAGGGCAATATTGATCATTCTGGTGTGAATCATGGAGGAATTTCACCTATGATTAACAAAAGGTTAAACAGTTTAGTGTCTTTTGATTCTGATGAAGATAGTTGCAATAGTCCTAAAGCACCGCACGATGTTCCCAAAGGATACTTAGCTGTCTATGTTGGTCCTGAGCTTAGGAGATTCATCATTCCCACAAGCTACCTTAGCCACTCACTTTTCAAAATGTTGTTGGAAAAAGCTGCTGATGAATTTGGTTTTAATCAGTGTGGTGGACTTACAATTCCATGTGAAATTGAAACCTTTAAATACCTTCTCAGCTGTATGGAGAATACTCAGCTTCATCATGATCAAACCTCTTCTGGAAACACAGGAACTGTGGAAGAGAGAATAAACTAG

Protein sequence

>Msa1224720

MDDAGVSKLTAKLKEIFQKWQVGYKEGNIDHSGVNHGGISPMINKRLNSLVSFDSDEDSCNSPKAPHDVPKGYLAVYVGPELRRFIIPTSYLSHSLFKMLLEKAADEFGFNQCGGLTIPCEIETFKYLLSCMENTQLHHDQTSSGNTGTVEERIN*