AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1266740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1266740 MtrunA17_Chr4g0070071 97.419 155 4 0 1 155 1 155 1.59e-113 318
Msa1266740 MtrunA17_Chr2g0301531 63.226 155 47 4 1 146 1 154 3.44e-63 191
Msa1266740 MtrunA17_Chr1g0147571 36.184 152 93 2 1 150 36 185 1.02e-24 94.7
Msa1266740 MtrunA17_Chr3g0139171 38.710 124 71 2 11 133 14 133 6.32e-23 89.0
Msa1266740 MtrunA17_Chr1g0178451 52.113 71 32 1 70 138 50 120 8.04e-21 82.4
Msa1266740 MtrunA17_Chr4g0000251 52.941 68 32 0 69 136 70 137 1.25e-20 82.8
Msa1266740 MtrunA17_Chr4g0036841 62.121 66 24 1 68 132 34 99 1.26e-20 81.3
Msa1266740 MtrunA17_Chr4g0036461 44.444 90 43 3 46 132 4 89 1.91e-20 80.5
Msa1266740 MtrunA17_Chr2g0281291 49.383 81 36 2 51 130 12 88 3.13e-20 79.7
Msa1266740 MtrunA17_Chr5g0405301 47.778 90 43 1 43 132 18 103 3.65e-20 80.1
Msa1266740 MtrunA17_Chr4g0009931 47.253 91 47 1 45 134 2 92 3.98e-20 79.7
Msa1266740 MtrunA17_Chr3g0145371 48.750 80 38 1 53 132 32 108 5.16e-20 79.7
Msa1266740 MtrunA17_Chr4g0036491 44.444 90 43 3 46 132 4 89 7.09e-20 79.0
Msa1266740 MtrunA17_Chr4g0036611 45.556 90 42 3 46 132 4 89 1.15e-19 78.2
Msa1266740 MtrunA17_Chr4g0036331 61.017 59 22 1 68 125 22 80 1.27e-19 78.2
Msa1266740 MtrunA17_Chr3g0121721 54.688 64 28 1 68 130 24 87 4.30e-19 77.0
Msa1266740 MtrunA17_Chr5g0396091 53.030 66 30 1 68 132 28 93 4.73e-19 77.0
Msa1266740 MtrunA17_Chr3g0139711 49.383 81 36 2 51 130 12 88 5.37e-19 76.6
Msa1266740 MtrunA17_Chr8g0372061 47.945 73 38 0 61 133 77 149 7.01e-19 77.8
Msa1266740 MtrunA17_Chr4g0036201 48.780 82 37 2 52 132 13 90 8.92e-19 75.9
Msa1266740 MtrunA17_Chr7g0266581 47.826 69 36 0 72 140 53 121 9.37e-19 77.0
Msa1266740 MtrunA17_Chr8g0358971 53.125 64 29 1 68 130 24 87 9.77e-19 75.9
Msa1266740 MtrunA17_Chr3g0121801 57.627 59 24 1 68 125 24 82 1.01e-18 75.9
Msa1266740 MtrunA17_Chr7g0266571 47.826 69 36 0 72 140 53 121 1.08e-18 77.0
Msa1266740 MtrunA17_Chr4g0036571 59.322 59 23 1 68 125 59 117 1.52e-18 76.6
Msa1266740 MtrunA17_Chr4g0036431 43.182 88 47 2 46 132 4 89 1.66e-18 75.5
Msa1266740 MtrunA17_Chr4g0036791 54.688 64 28 1 68 130 26 89 1.99e-18 75.1
Msa1266740 MtrunA17_Chr8g0359011 57.627 59 24 1 68 125 2 60 2.14e-18 74.3
Msa1266740 MtrunA17_Chr4g0036821 54.545 66 29 1 68 132 25 90 3.11e-18 74.7
Msa1266740 MtrunA17_Chr4g0036181 46.591 88 45 2 46 132 61 147 3.50e-18 76.3
Msa1266740 MtrunA17_Chr4g0036551 51.515 66 31 1 68 132 19 84 3.60e-18 74.3
Msa1266740 MtrunA17_Chr4g0036801 53.030 66 30 1 68 132 25 90 4.21e-18 74.3
Msa1266740 MtrunA17_Chr3g0121701 47.500 80 37 2 52 130 12 87 4.62e-18 74.3
Msa1266740 MtrunA17_Chr4g0036811 46.512 86 44 2 46 130 4 88 4.70e-18 74.3
Msa1266740 MtrunA17_Chr3g0121731 47.500 80 37 2 52 130 12 87 4.72e-18 74.3
Msa1266740 MtrunA17_Chr4g0036681 51.515 66 31 1 68 132 24 89 6.32e-18 73.9
Msa1266740 MtrunA17_Chr4g0036531 51.515 66 31 1 68 132 24 89 6.74e-18 73.9
Msa1266740 MtrunA17_Chr8g0359001 59.259 54 21 1 68 120 24 77 7.63e-18 73.2
Msa1266740 MtrunA17_Chr4g0036831 46.512 86 44 2 46 130 4 88 8.60e-18 73.6
Msa1266740 MtrunA17_Chr4g0036751 50.000 74 36 1 61 133 12 85 1.06e-17 73.2
Msa1266740 MtrunA17_Chr4g0036621 55.932 59 25 1 68 125 18 76 1.36e-17 72.8
Msa1266740 MtrunA17_Chr8g0358981 51.562 64 30 1 68 130 24 87 1.63e-17 72.8
Msa1266740 MtrunA17_Chr8g0358991 53.030 66 30 1 68 132 24 89 1.84e-17 72.8
Msa1266740 MtrunA17_Chr4g0036631 50.000 66 32 1 68 132 19 84 1.98e-17 72.4
Msa1266740 MtrunA17_Chr4g0036581 48.485 66 33 1 68 132 24 89 2.46e-17 72.4
Msa1266740 MtrunA17_Chr5g0396081 57.576 66 25 3 61 125 13 76 2.73e-17 72.0
Msa1266740 MtrunA17_Chr4g0036271 54.237 59 26 1 68 125 22 80 3.51e-17 72.0
Msa1266740 MtrunA17_Chr4g0036161 48.529 68 35 0 66 133 33 100 3.67e-17 72.0
Msa1266740 MtrunA17_Chr4g0036691 54.237 59 26 1 68 125 19 77 3.70e-17 71.6
Msa1266740 MtrunA17_Chr3g0121711 51.515 66 31 1 68 132 24 89 4.12e-17 71.6
Msa1266740 MtrunA17_Chr4g0036391 56.897 58 24 1 69 125 20 77 4.21e-17 71.6
Msa1266740 MtrunA17_Chr3g0121741 51.515 66 31 1 68 132 24 89 4.49e-17 71.6
Msa1266740 MtrunA17_Chr4g0036221 54.688 64 27 2 68 130 25 87 4.74e-17 71.6
Msa1266740 MtrunA17_Chr4g0036651 40.909 88 49 2 46 132 4 89 5.69e-17 71.6
Msa1266740 MtrunA17_Chr4g0036601 51.562 64 30 1 68 130 20 83 6.24e-17 71.2
Msa1266740 MtrunA17_Chr3g0121761 51.515 66 31 1 68 132 24 89 7.28e-17 71.2
Msa1266740 MtrunA17_Chr7g0214201 46.970 66 35 0 69 134 67 132 7.30e-17 73.2
Msa1266740 MtrunA17_Chr4g0036471 54.237 59 26 1 68 125 20 78 8.43e-17 70.9
Msa1266740 MtrunA17_Chr4g0036641 54.237 59 26 1 68 125 20 78 8.43e-17 70.9
Msa1266740 MtrunA17_Chr4g0036671 54.237 59 26 1 68 125 20 78 8.80e-17 70.9
Msa1266740 MtrunA17_Chr3g0121781 51.515 66 31 1 68 132 24 89 9.03e-17 70.9
Msa1266740 MtrunA17_Chr4g0036561 51.515 66 31 1 68 132 19 84 9.64e-17 70.9
Msa1266740 MtrunA17_Chr4g0036861 54.839 62 27 1 68 128 56 117 1.06e-16 71.6
Msa1266740 MtrunA17_Chr4g0036241 51.562 64 30 1 68 130 25 88 1.08e-16 70.9
Msa1266740 MtrunA17_Chr4g0036211 44.444 90 48 2 46 134 142 230 1.20e-16 74.3
Msa1266740 MtrunA17_Chr4g0036211 46.667 75 38 2 46 119 4 77 2.32e-14 68.2
Msa1266740 MtrunA17_Chr4g0036191 53.125 64 27 2 68 130 25 86 1.28e-16 70.5
Msa1266740 MtrunA17_Chr4g0036141 44.444 72 40 0 67 138 35 106 1.34e-16 71.6
Msa1266740 MtrunA17_Chr4g0036441 52.542 59 27 1 68 125 19 77 1.47e-16 70.1
Msa1266740 MtrunA17_Chr1g0179511 43.836 73 41 0 57 129 7 79 1.53e-16 71.2
Msa1266740 MtrunA17_Chr3g0139731 53.125 64 29 1 68 130 63 126 1.54e-16 71.6
Msa1266740 MtrunA17_Chr4g0036411 48.148 81 35 3 46 125 4 78 1.64e-16 70.1
Msa1266740 MtrunA17_Chr8g0346181 41.026 78 43 2 69 143 27 104 2.62e-16 70.1
Msa1266740 MtrunA17_Chr4g0036401 46.970 66 34 1 68 132 24 89 3.01e-16 69.7
Msa1266740 MtrunA17_Chr4g0036761 54.237 59 26 1 68 125 22 80 3.02e-16 69.7
Msa1266740 MtrunA17_Chr4g0036501 54.237 59 26 1 68 125 20 78 3.38e-16 69.3
Msa1266740 MtrunA17_Chr4g0036521 51.471 68 31 2 68 133 31 98 3.80e-16 69.7
Msa1266740 MtrunA17_Chr4g0036661 55.172 58 25 1 69 125 20 77 4.00e-16 68.9
Msa1266740 MtrunA17_Chr3g0139701 54.237 59 26 1 68 125 82 140 5.19e-16 70.5
Msa1266740 MtrunA17_Chr3g0145411 53.425 73 27 3 68 138 33 100 5.52e-16 69.3
Msa1266740 MtrunA17_Chr3g0121811 50.000 70 30 2 52 120 13 78 6.15e-16 68.9
Msa1266740 MtrunA17_Chr3g0145401 51.562 64 30 1 68 130 44 107 8.91e-16 68.9
Msa1266740 MtrunA17_Chr1g0156341 46.970 66 35 0 64 129 32 97 9.08e-16 69.7
Msa1266740 MtrunA17_Chr4g0036541 54.237 59 26 1 68 125 19 77 1.03e-15 68.2
Msa1266740 MtrunA17_Chr3g0127561 39.744 78 45 1 68 143 32 109 1.08e-15 68.9
Msa1266740 MtrunA17_Chr4g0036231 50.000 72 31 2 50 120 19 86 1.08e-15 68.2
Msa1266740 MtrunA17_Chr4g0036421 44.444 81 37 2 46 125 4 77 1.21e-15 67.8
Msa1266740 MtrunA17_Chr4g0000241 50.794 63 29 1 69 129 62 124 1.84e-15 69.3
Msa1266740 MtrunA17_Chr3g0142421 46.970 66 35 0 68 133 41 106 1.91e-15 67.8
Msa1266740 MtrunA17_Chr7g0276101 40.789 76 42 2 69 141 23 98 6.05e-15 66.6
Msa1266740 MtrunA17_Chr5g0396071 46.970 66 34 1 68 132 20 85 6.33e-15 66.2
Msa1266740 MtrunA17_Chr4g0036341 44.304 79 42 2 61 138 13 90 6.52e-15 66.2
Msa1266740 MtrunA17_Chr8g0358871 38.750 80 46 1 68 144 34 113 7.76e-15 66.6
Msa1266740 MtrunA17_Chr4g0011821 51.667 60 26 1 69 125 79 138 1.90e-14 66.6
Msa1266740 MtrunA17_Chr4g0036891 48.529 68 33 2 68 133 26 93 2.43e-14 64.7
Msa1266740 MtrunA17_Chr1g0199531 43.590 78 44 0 52 129 33 110 3.56e-14 64.7
Msa1266740 MtrunA17_Chr7g0264401 46.479 71 38 0 66 136 37 107 7.03e-14 63.9
Msa1266740 MtrunA17_Chr4g0036711 46.269 67 35 1 69 134 15 81 7.58e-14 63.2
Msa1266740 MtrunA17_Chr8g0386121 44.615 65 36 0 70 134 47 111 1.69e-13 63.2
Msa1266740 MtrunA17_Chr3g0121751 50.820 61 25 2 51 111 70 125 3.46e-13 63.5
Msa1266740 MtrunA17_Chr1g0181011 39.286 84 48 1 53 136 76 156 3.75e-13 63.5
Msa1266740 MtrunA17_Chr6g0481791 40.299 67 39 1 68 133 33 99 4.28e-13 61.6
Msa1266740 MtrunA17_Chr7g0230411 40.260 77 41 2 61 134 69 143 4.55e-13 62.8
Msa1266740 MtrunA17_Chr8g0386131 42.029 69 40 0 70 138 50 118 4.99e-13 62.8
Msa1266740 MtrunA17_Chr3g0131991 38.235 68 42 0 69 136 53 120 5.44e-13 62.8
Msa1266740 MtrunA17_Chr8g0386111 42.029 69 40 0 70 138 46 114 6.14e-13 62.4
Msa1266740 MtrunA17_Chr8g0389151 40.506 79 41 1 48 126 25 97 1.69e-12 61.2
Msa1266740 MtrunA17_Chr2g0311341 36.986 73 44 1 61 133 52 122 2.96e-12 60.5
Msa1266740 MtrunA17_Chr8g0389141 33.654 104 61 2 43 146 27 122 1.14e-11 59.3
Msa1266740 MtrunA17_Chr7g0264551 33.696 92 56 2 32 121 18 106 1.56e-11 58.2
Msa1266740 MtrunA17_Chr4g0036701 51.852 54 25 1 62 115 26 78 1.59e-11 57.4
Msa1266740 MtrunA17_Chr2g0327281 37.079 89 49 2 37 121 16 101 1.98e-11 58.2
Msa1266740 MtrunA17_Chr4g0028491 38.961 77 40 2 68 142 37 108 2.87e-11 57.4
Msa1266740 MtrunA17_Chr8g0385011 42.466 73 29 1 69 128 3 75 3.35e-11 57.0
Msa1266740 MtrunA17_Chr3g0130911 44.118 68 30 1 69 128 9 76 4.09e-11 56.6
Msa1266740 MtrunA17_Chr4g0028481 46.970 66 31 1 68 129 37 102 4.30e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1266740 AT5G20810.1 58.537 164 56 4 1 152 1 164 7.43e-59 180
Msa1266740 AT5G20810.2 60.645 155 49 4 1 143 1 155 5.33e-57 176
Msa1266740 AT3G43120.1 56.790 162 58 5 1 152 1 160 3.17e-55 171
Msa1266740 AT4G31320.1 49.425 87 38 1 69 155 80 160 6.89e-21 84.7
Msa1266740 AT2G24400.1 58.462 65 27 0 69 133 64 128 8.64e-21 84.0
Msa1266740 AT2G21220.1 45.000 100 49 2 32 130 7 101 1.72e-19 78.6
Msa1266740 AT4G34800.1 53.947 76 31 2 58 129 10 85 2.91e-19 77.8
Msa1266740 AT2G18010.1 53.846 65 30 0 66 130 45 109 8.18e-19 77.0
Msa1266740 AT3G61900.1 39.815 108 61 2 45 148 6 113 1.74e-18 77.0
Msa1266740 AT3G51200.1 45.161 93 42 2 37 129 17 100 4.32e-18 75.1
Msa1266740 AT1G19830.1 38.889 108 62 1 29 132 4 111 4.33e-18 75.5
Msa1266740 AT2G46690.1 49.275 69 32 1 68 133 23 91 5.57e-18 75.1
Msa1266740 AT4G38840.1 58.462 65 25 2 68 130 32 96 1.46e-17 73.6
Msa1266740 AT3G20220.1 46.575 73 38 1 58 129 36 108 1.80e-17 73.9
Msa1266740 AT4G34790.1 50.685 73 30 3 69 138 39 108 1.92e-17 73.6
Msa1266740 AT5G66260.1 50.000 64 32 0 69 132 34 97 2.15e-17 73.2
Msa1266740 AT1G75580.1 40.659 91 54 0 43 133 18 108 3.29e-17 72.8
Msa1266740 AT2G21210.1 51.429 70 31 2 69 135 28 97 4.36e-17 72.4
Msa1266740 AT4G34760.1 50.769 65 32 0 66 130 40 104 7.19e-17 72.0
Msa1266740 AT4G00880.1 50.685 73 32 2 69 137 28 100 1.55e-16 71.6
Msa1266740 AT4G38860.1 53.030 66 30 1 66 130 37 102 2.16e-16 70.9
Msa1266740 AT2G21210.2 56.667 60 23 2 69 125 95 154 2.36e-16 72.0
Msa1266740 AT4G34810.1 50.746 67 28 2 69 130 48 114 3.25e-16 70.5
Msa1266740 AT4G34770.1 43.678 87 46 2 52 135 18 104 4.41e-16 69.7
Msa1266740 AT4G38825.1 50.704 71 28 3 70 138 24 89 5.26e-16 69.3
Msa1266740 AT4G36110.1 49.206 63 32 0 68 130 39 101 1.06e-15 68.9
Msa1266740 AT2G37030.1 48.485 66 34 0 66 131 46 111 1.59e-15 68.9
Msa1266740 AT5G10990.1 50.000 62 31 0 65 126 37 98 1.84e-15 69.3
Msa1266740 AT3G03820.1 50.704 71 31 2 68 134 26 96 2.83e-15 67.4
Msa1266740 AT1G75590.1 48.387 62 32 0 65 126 38 99 4.05e-15 68.6
Msa1266740 AT5G18050.1 50.746 67 31 2 61 125 16 82 5.43e-15 66.6
Msa1266740 AT1G56150.1 35.714 84 54 0 49 132 18 101 8.30e-15 66.6
Msa1266740 AT5G18030.1 54.237 59 25 2 69 125 22 80 1.31e-14 65.5
Msa1266740 AT3G60690.1 44.444 72 37 1 66 134 85 156 1.45e-14 67.8
Msa1266740 AT2G16580.1 47.059 68 36 0 66 133 41 108 1.71e-14 65.9
Msa1266740 AT5G18010.1 50.746 67 31 2 61 125 16 82 2.19e-14 65.1
Msa1266740 AT5G18020.1 49.254 67 32 2 61 125 16 82 2.22e-14 65.1
Msa1266740 AT2G36210.1 38.710 93 52 2 49 140 38 126 3.68e-14 65.9
Msa1266740 AT3G03850.1 48.649 74 32 3 64 134 23 93 3.78e-14 64.7
Msa1266740 AT3G12830.1 35.366 82 53 0 52 133 33 114 6.87e-14 65.1
Msa1266740 AT5G18060.1 53.448 58 25 2 70 125 25 82 7.35e-14 63.5
Msa1266740 AT1G19840.1 48.387 62 32 0 65 126 37 98 1.05e-13 65.1
Msa1266740 AT4G34780.1 53.226 62 25 2 68 125 27 88 1.19e-13 63.5
Msa1266740 AT5G53590.1 43.590 78 38 2 68 139 45 122 1.29e-13 64.3
Msa1266740 AT3G53250.1 43.939 66 34 1 66 131 49 111 2.49e-13 63.2
Msa1266740 AT3G03840.1 54.237 59 24 2 70 125 28 86 3.52e-13 62.0
Msa1266740 AT4G34750.2 35.897 78 47 1 68 145 36 110 6.61e-13 62.8
Msa1266740 AT4G34750.1 35.897 78 47 1 68 145 36 110 6.61e-13 62.8
Msa1266740 AT4G38850.1 46.667 60 28 2 70 125 22 81 1.01e-12 60.8
Msa1266740 AT3G53250.2 43.939 66 34 1 66 131 80 142 1.57e-12 62.0
Msa1266740 AT4G22620.1 44.118 68 34 2 61 125 72 138 2.00e-12 61.6
Msa1266740 AT4G09530.1 38.028 71 40 2 70 136 29 99 2.97e-12 60.1
Msa1266740 AT4G13790.1 49.123 57 26 2 68 121 24 80 3.11e-12 59.7
Msa1266740 AT1G16510.1 36.923 65 41 0 69 133 54 118 5.86e-12 60.1
Msa1266740 AT1G20470.1 43.284 67 37 1 71 137 25 90 9.22e-12 59.7
Msa1266740 AT5G50760.1 41.270 63 37 0 72 134 58 120 1.77e-11 59.7
Msa1266740 AT2G28085.1 31.579 114 66 4 28 130 8 120 1.80e-11 58.5
Msa1266740 AT4G12410.1 42.857 63 33 1 69 128 76 138 2.36e-11 58.9

Find 40 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGCTGAGAAGGTATTTAA+AGG 0.276472 8_2:+71731167 None:intergenic
GGAACATCGTGCGGTGCTTT+AGG 0.291352 8_2:+71731351 None:intergenic
GTTATCTTCTATTCAATATC+AGG 0.295590 8_2:-71732020 Msa1266740:intron
GAGGGCAATATTGATCATTC+TGG 0.379305 8_2:-71731462 Msa1266740:CDS
GAAGATAACATTAGATATGA+AGG 0.399265 8_2:+71732034 None:intergenic
TGGCAGAGCGTTTGTTAAGC+TGG 0.411349 8_2:+71732076 None:intergenic
TCTATGTTGGTCCTGAGCTT+AGG 0.426326 8_2:-71731311 Msa1266740:CDS
TAAATACCTTCTCAGCTGTA+TGG 0.429520 8_2:-71731163 Msa1266740:CDS
ATAGACAGCTAAGTATCCTT+TGG 0.430579 8_2:+71731329 None:intergenic
TCTGTATTTAGTGTGACATA+AGG 0.442791 8_2:-71731623 Msa1266740:intron
AAATCTGCTAAACTTGATCC+TGG 0.454410 8_2:-71731596 Msa1266740:five_prime_UTR
TGAAAGAAATATTTCAAAAG+TGG 0.457045 8_2:-71731500 Msa1266740:CDS
TAGACAGCTAAGTATCCTTT+GGG 0.468981 8_2:+71731330 None:intergenic
TGGCAAAAGCTTATCATCAA+TGG 0.473791 8_2:-71731556 Msa1266740:exon
TTTCACCTATGATTAACAAA+AGG 0.486292 8_2:-71731422 Msa1266740:CDS
AGAGGTGCAAATCATAGTGC+TGG 0.508610 8_2:-71730954 Msa1266740:three_prime_UTR
GAGCGTTTGTTAAGCTGGAA+TGG 0.513920 8_2:+71732081 None:intergenic
AACTAGTATGATACTTAAAG+AGG 0.531529 8_2:-71730972 Msa1266740:exon
TTATCATCAATGGACGATGC+TGG 0.539963 8_2:-71731546 Msa1266740:exon
GAGTGGCTAAGGTAGCTTGT+GGG 0.551541 8_2:+71731276 None:intergenic
GGATACTTAGCTGTCTATGT+TGG 0.555421 8_2:-71731324 Msa1266740:CDS
ACAAGAGTTAAAAGTATCCC+AGG 0.556086 8_2:+71731578 None:intergenic
TGAAGGGATGTCAATGAGAT+CGG 0.558302 8_2:+71732051 None:intergenic
TATTCTCCATACAGCTGAGA+AGG 0.559878 8_2:+71731157 None:intergenic
AATATTTCAAAAGTGGCAAG+TGG 0.559917 8_2:-71731493 Msa1266740:CDS
GATCATTCTGGTGTGAATCA+TGG 0.562412 8_2:-71731450 Msa1266740:CDS
ATGATGAATCTCCTAAGCTC+AGG 0.564919 8_2:+71731300 None:intergenic
GTGGCAAGTGGGTTATAAAG+AGG 0.566612 8_2:-71731481 Msa1266740:CDS
AAGATAACATTAGATATGAA+GGG 0.591038 8_2:+71732035 None:intergenic
AATCTGCTAAACTTGATCCT+GGG 0.596689 8_2:-71731595 Msa1266740:five_prime_UTR
TGGCAAGTGGGTTATAAAGA+GGG 0.604345 8_2:-71731480 Msa1266740:CDS
GAAATTGCAGCTGGAAACAC+AGG 0.604667 8_2:-71731014 Msa1266740:intron
TTAAAGGTTTCAATTTCACA+TGG 0.605541 8_2:+71731183 None:intergenic
ATATTTCAAAAGTGGCAAGT+GGG 0.643378 8_2:-71731492 Msa1266740:CDS
TGAGTGGCTAAGGTAGCTTG+TGG 0.646992 8_2:+71731275 None:intergenic
GGATGTCAATGAGATCGGAA+TGG 0.652039 8_2:+71732056 None:intergenic
TATCCTTTGGGAACATCGTG+CGG 0.653439 8_2:+71731342 None:intergenic
GCACCGCACGATGTTCCCAA+AGG 0.669257 8_2:-71731345 Msa1266740:CDS
AGCTGGAAACACAGGAACTG+TGG 0.675127 8_2:-71731006 Msa1266740:CDS
CATTCTGGTGTGAATCATGG+AGG 0.741290 8_2:-71731447 Msa1266740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTTTATATAATTTCATAT+AGG - chr8_2:71731955-71731974 Msa1266740:intron 10.0%
!! TAATTAGTTAAAAAAAGAAA+AGG + chr8_2:71732005-71732024 None:intergenic 10.0%
!! TACTTTATATAATTTCATAT+AGG - chr8_2:71731955-71731974 Msa1266740:intron 10.0%
!! TAATTAGTTAAAAAAAGAAA+AGG + chr8_2:71732005-71732024 None:intergenic 10.0%
!!! TTTAAAATTTCTGTAATCAT+GGG + chr8_2:71731197-71731216 None:intergenic 15.0%
!!! ATTTAAAATTTCTGTAATCA+TGG + chr8_2:71731198-71731217 None:intergenic 15.0%
!!! TTTAAAATTTCTGTAATCAT+GGG + chr8_2:71731197-71731216 None:intergenic 15.0%
!!! ATTTAAAATTTCTGTAATCA+TGG + chr8_2:71731198-71731217 None:intergenic 15.0%
!! AAGATAACATTAGATATGAA+GGG + chr8_2:71731020-71731039 None:intergenic 20.0%
!! TAAATACAGAGATGTTATAA+AGG + chr8_2:71731419-71731438 None:intergenic 20.0%
!! TGAAAGAAATATTTCAAAAG+TGG - chr8_2:71731552-71731571 Msa1266740:exon 20.0%
!!! TTTAACCTTTTGTTAATCAT+AGG + chr8_2:71731638-71731657 None:intergenic 20.0%
!! TAAAGTAATTAACTTACAAG+AGG + chr8_2:71731942-71731961 None:intergenic 20.0%
!! TTAACTAATTATTCGACTAT+TGG - chr8_2:71732014-71732033 Msa1266740:intron 20.0%
!!! AATGATGTTTCTCAATTTTA+TGG + chr8_2:71730932-71730951 None:intergenic 20.0%
!! AAGATAACATTAGATATGAA+GGG + chr8_2:71731020-71731039 None:intergenic 20.0%
!! TAAATACAGAGATGTTATAA+AGG + chr8_2:71731419-71731438 None:intergenic 20.0%
!! TGAAAGAAATATTTCAAAAG+TGG - chr8_2:71731552-71731571 Msa1266740:exon 20.0%
!!! TTTAACCTTTTGTTAATCAT+AGG + chr8_2:71731638-71731657 None:intergenic 20.0%
!! TAAAGTAATTAACTTACAAG+AGG + chr8_2:71731942-71731961 None:intergenic 20.0%
!! TTAACTAATTATTCGACTAT+TGG - chr8_2:71732014-71732033 Msa1266740:intron 20.0%
! GAAGATAACATTAGATATGA+AGG + chr8_2:71731021-71731040 None:intergenic 25.0%
! GTTATCTTCTATTCAATATC+AGG - chr8_2:71731032-71731051 Msa1266740:intron 25.0%
! CATTGTAGTGTTAACAAAAA+AGG + chr8_2:71731091-71731110 None:intergenic 25.0%
!! GTTTTCTACTATTCTATGAA+TGG - chr8_2:71731147-71731166 Msa1266740:CDS 25.0%
! TTTCACCTATGATTAACAAA+AGG - chr8_2:71731630-71731649 Msa1266740:intron 25.0%
! TTAAAGGTTTCAATTTCACA+TGG + chr8_2:71731872-71731891 None:intergenic 25.0%
! AACTAGTATGATACTTAAAG+AGG - chr8_2:71732080-71732099 Msa1266740:five_prime_UTR 25.0%
! GAAGATAACATTAGATATGA+AGG + chr8_2:71731021-71731040 None:intergenic 25.0%
! GTTATCTTCTATTCAATATC+AGG - chr8_2:71731032-71731051 Msa1266740:intron 25.0%
! CATTGTAGTGTTAACAAAAA+AGG + chr8_2:71731091-71731110 None:intergenic 25.0%
!! GTTTTCTACTATTCTATGAA+TGG - chr8_2:71731147-71731166 Msa1266740:CDS 25.0%
! TTTCACCTATGATTAACAAA+AGG - chr8_2:71731630-71731649 Msa1266740:intron 25.0%
! TTAAAGGTTTCAATTTCACA+TGG + chr8_2:71731872-71731891 None:intergenic 25.0%
! AACTAGTATGATACTTAAAG+AGG - chr8_2:71732080-71732099 Msa1266740:five_prime_UTR 25.0%
! CAGGTAACTGCTAAATTTTA+TGG + chr8_2:71731257-71731276 None:intergenic 30.0%
TCTGTATTTAGTGTGACATA+AGG - chr8_2:71731429-71731448 Msa1266740:CDS 30.0%
AATATTTCAAAAGTGGCAAG+TGG - chr8_2:71731559-71731578 Msa1266740:exon 30.0%
ATATTTCAAAAGTGGCAAGT+GGG - chr8_2:71731560-71731579 Msa1266740:five_prime_UTR 30.0%
!! ACAACATTTTGAAAAGTGAG+TGG + chr8_2:71731796-71731815 None:intergenic 30.0%
!! CTCACTTTTCAAAATGTTGT+TGG - chr8_2:71731796-71731815 Msa1266740:intron 30.0%
!!! GAATTTGGTTTTAATCAGTG+TGG - chr8_2:71731833-71731852 Msa1266740:intron 30.0%
! CAGGTAACTGCTAAATTTTA+TGG + chr8_2:71731257-71731276 None:intergenic 30.0%
TCTGTATTTAGTGTGACATA+AGG - chr8_2:71731429-71731448 Msa1266740:CDS 30.0%
AATATTTCAAAAGTGGCAAG+TGG - chr8_2:71731559-71731578 Msa1266740:exon 30.0%
ATATTTCAAAAGTGGCAAGT+GGG - chr8_2:71731560-71731579 Msa1266740:five_prime_UTR 30.0%
!! ACAACATTTTGAAAAGTGAG+TGG + chr8_2:71731796-71731815 None:intergenic 30.0%
!! CTCACTTTTCAAAATGTTGT+TGG - chr8_2:71731796-71731815 Msa1266740:intron 30.0%
!!! GAATTTGGTTTTAATCAGTG+TGG - chr8_2:71731833-71731852 Msa1266740:intron 30.0%
GGATCTATGATCATCTATCT+TGG - chr8_2:71731168-71731187 Msa1266740:CDS 35.0%
CTTTAGATTGAAGACGTGTT+AGG + chr8_2:71731345-71731364 None:intergenic 35.0%
AAATCTGCTAAACTTGATCC+TGG - chr8_2:71731456-71731475 Msa1266740:CDS 35.0%
AATCTGCTAAACTTGATCCT+GGG - chr8_2:71731457-71731476 Msa1266740:CDS 35.0%
!! ACAAGAGTTAAAAGTATCCC+AGG + chr8_2:71731477-71731496 None:intergenic 35.0%
!! TGGGATACTTTTAACTCTTG+TGG - chr8_2:71731476-71731495 Msa1266740:CDS 35.0%
TGGCAAAAGCTTATCATCAA+TGG - chr8_2:71731496-71731515 Msa1266740:CDS 35.0%
TAGACAGCTAAGTATCCTTT+GGG + chr8_2:71731725-71731744 None:intergenic 35.0%
ATAGACAGCTAAGTATCCTT+TGG + chr8_2:71731726-71731745 None:intergenic 35.0%
!! TTTTGAAAAGTGAGTGGCTA+AGG + chr8_2:71731790-71731809 None:intergenic 35.0%
GAAAAAGCTGCTGATGAATT+TGG - chr8_2:71731818-71731837 Msa1266740:intron 35.0%
!!! TTTGGTTTTAATCAGTGTGG+TGG - chr8_2:71731836-71731855 Msa1266740:intron 35.0%
!! ACAGCTGAGAAGGTATTTAA+AGG + chr8_2:71731888-71731907 None:intergenic 35.0%
TAAATACCTTCTCAGCTGTA+TGG - chr8_2:71731889-71731908 Msa1266740:intron 35.0%
GGATCTATGATCATCTATCT+TGG - chr8_2:71731168-71731187 Msa1266740:CDS 35.0%
CTTTAGATTGAAGACGTGTT+AGG + chr8_2:71731345-71731364 None:intergenic 35.0%
AAATCTGCTAAACTTGATCC+TGG - chr8_2:71731456-71731475 Msa1266740:CDS 35.0%
AATCTGCTAAACTTGATCCT+GGG - chr8_2:71731457-71731476 Msa1266740:CDS 35.0%
!! ACAAGAGTTAAAAGTATCCC+AGG + chr8_2:71731477-71731496 None:intergenic 35.0%
!! TGGGATACTTTTAACTCTTG+TGG - chr8_2:71731476-71731495 Msa1266740:CDS 35.0%
TGGCAAAAGCTTATCATCAA+TGG - chr8_2:71731496-71731515 Msa1266740:CDS 35.0%
TAGACAGCTAAGTATCCTTT+GGG + chr8_2:71731725-71731744 None:intergenic 35.0%
ATAGACAGCTAAGTATCCTT+TGG + chr8_2:71731726-71731745 None:intergenic 35.0%
!! TTTTGAAAAGTGAGTGGCTA+AGG + chr8_2:71731790-71731809 None:intergenic 35.0%
GAAAAAGCTGCTGATGAATT+TGG - chr8_2:71731818-71731837 Msa1266740:intron 35.0%
!!! TTTGGTTTTAATCAGTGTGG+TGG - chr8_2:71731836-71731855 Msa1266740:intron 35.0%
!! ACAGCTGAGAAGGTATTTAA+AGG + chr8_2:71731888-71731907 None:intergenic 35.0%
TAAATACCTTCTCAGCTGTA+TGG - chr8_2:71731889-71731908 Msa1266740:intron 35.0%
TGAAGGGATGTCAATGAGAT+CGG + chr8_2:71731004-71731023 None:intergenic 40.0%
TTATCATCAATGGACGATGC+TGG - chr8_2:71731506-71731525 Msa1266740:CDS 40.0%
!! TGGCAAGTGGGTTATAAAGA+GGG - chr8_2:71731572-71731591 Msa1266740:five_prime_UTR 40.0%
GAGGGCAATATTGATCATTC+TGG - chr8_2:71731590-71731609 Msa1266740:five_prime_UTR 40.0%
GATCATTCTGGTGTGAATCA+TGG - chr8_2:71731602-71731621 Msa1266740:five_prime_UTR 40.0%
GGATACTTAGCTGTCTATGT+TGG - chr8_2:71731728-71731747 Msa1266740:intron 40.0%
ATGATGAATCTCCTAAGCTC+AGG + chr8_2:71731755-71731774 None:intergenic 40.0%
TATTCTCCATACAGCTGAGA+AGG + chr8_2:71731898-71731917 None:intergenic 40.0%
ACTATTGGTGAAATTGCAGC+TGG - chr8_2:71732029-71732048 Msa1266740:five_prime_UTR 40.0%
TGAAGGGATGTCAATGAGAT+CGG + chr8_2:71731004-71731023 None:intergenic 40.0%
TTATCATCAATGGACGATGC+TGG - chr8_2:71731506-71731525 Msa1266740:CDS 40.0%
!! TGGCAAGTGGGTTATAAAGA+GGG - chr8_2:71731572-71731591 Msa1266740:five_prime_UTR 40.0%
GAGGGCAATATTGATCATTC+TGG - chr8_2:71731590-71731609 Msa1266740:five_prime_UTR 40.0%
GATCATTCTGGTGTGAATCA+TGG - chr8_2:71731602-71731621 Msa1266740:five_prime_UTR 40.0%
GGATACTTAGCTGTCTATGT+TGG - chr8_2:71731728-71731747 Msa1266740:intron 40.0%
ATGATGAATCTCCTAAGCTC+AGG + chr8_2:71731755-71731774 None:intergenic 40.0%
TATTCTCCATACAGCTGAGA+AGG + chr8_2:71731898-71731917 None:intergenic 40.0%
ACTATTGGTGAAATTGCAGC+TGG - chr8_2:71732029-71732048 Msa1266740:five_prime_UTR 40.0%
! GAGCGTTTGTTAAGCTGGAA+TGG + chr8_2:71730974-71730993 None:intergenic 45.0%
GGATGTCAATGAGATCGGAA+TGG + chr8_2:71730999-71731018 None:intergenic 45.0%
TGTTAACTAGTGTGCAGTGC+AGG + chr8_2:71731276-71731295 None:intergenic 45.0%
!! GTGGCAAGTGGGTTATAAAG+AGG - chr8_2:71731571-71731590 Msa1266740:five_prime_UTR 45.0%
CATTCTGGTGTGAATCATGG+AGG - chr8_2:71731605-71731624 Msa1266740:five_prime_UTR 45.0%
! TATCCTTTGGGAACATCGTG+CGG + chr8_2:71731713-71731732 None:intergenic 45.0%
TCTATGTTGGTCCTGAGCTT+AGG - chr8_2:71731741-71731760 Msa1266740:intron 45.0%
GAAATTGCAGCTGGAAACAC+AGG - chr8_2:71732038-71732057 Msa1266740:five_prime_UTR 45.0%
! AGAGGTGCAAATCATAGTGC+TGG - chr8_2:71732098-71732117 Msa1266740:five_prime_UTR 45.0%
! GAGCGTTTGTTAAGCTGGAA+TGG + chr8_2:71730974-71730993 None:intergenic 45.0%
GGATGTCAATGAGATCGGAA+TGG + chr8_2:71730999-71731018 None:intergenic 45.0%
TGTTAACTAGTGTGCAGTGC+AGG + chr8_2:71731276-71731295 None:intergenic 45.0%
!! GTGGCAAGTGGGTTATAAAG+AGG - chr8_2:71731571-71731590 Msa1266740:five_prime_UTR 45.0%
CATTCTGGTGTGAATCATGG+AGG - chr8_2:71731605-71731624 Msa1266740:five_prime_UTR 45.0%
! TATCCTTTGGGAACATCGTG+CGG + chr8_2:71731713-71731732 None:intergenic 45.0%
TCTATGTTGGTCCTGAGCTT+AGG - chr8_2:71731741-71731760 Msa1266740:intron 45.0%
GAAATTGCAGCTGGAAACAC+AGG - chr8_2:71732038-71732057 Msa1266740:five_prime_UTR 45.0%
! AGAGGTGCAAATCATAGTGC+TGG - chr8_2:71732098-71732117 Msa1266740:five_prime_UTR 45.0%
! TGGCAGAGCGTTTGTTAAGC+TGG + chr8_2:71730979-71730998 None:intergenic 50.0%
! GAGTGGCTAAGGTAGCTTGT+GGG + chr8_2:71731779-71731798 None:intergenic 50.0%
TGAGTGGCTAAGGTAGCTTG+TGG + chr8_2:71731780-71731799 None:intergenic 50.0%
AGCTGGAAACACAGGAACTG+TGG - chr8_2:71732046-71732065 Msa1266740:five_prime_UTR 50.0%
! TGGCAGAGCGTTTGTTAAGC+TGG + chr8_2:71730979-71730998 None:intergenic 50.0%
! GAGTGGCTAAGGTAGCTTGT+GGG + chr8_2:71731779-71731798 None:intergenic 50.0%
TGAGTGGCTAAGGTAGCTTG+TGG + chr8_2:71731780-71731799 None:intergenic 50.0%
AGCTGGAAACACAGGAACTG+TGG - chr8_2:71732046-71732065 Msa1266740:five_prime_UTR 50.0%
GGAACATCGTGCGGTGCTTT+AGG + chr8_2:71731704-71731723 None:intergenic 55.0%
GGAACATCGTGCGGTGCTTT+AGG + chr8_2:71731704-71731723 None:intergenic 55.0%
GCACCGCACGATGTTCCCAA+AGG - chr8_2:71731707-71731726 Msa1266740:intron 60.0%
GCACCGCACGATGTTCCCAA+AGG - chr8_2:71731707-71731726 Msa1266740:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 71730931 71732143 71730931 ID=Msa1266740;Name=Msa1266740
chr8_2 mRNA 71730931 71732143 71730931 ID=Msa1266740-mRNA-1;Parent=Msa1266740;Name=Msa1266740-mRNA-1;_AED=0.01;_eAED=0.01;_QI=198|1|1|1|0.5|0.33|3|58|155
chr8_2 exon 71730931 71731026 71730931 ID=Msa1266740-mRNA-1:exon:24093;Parent=Msa1266740-mRNA-1
chr8_2 exon 71731130 71731634 71731130 ID=Msa1266740-mRNA-1:exon:24092;Parent=Msa1266740-mRNA-1
chr8_2 exon 71732021 71732143 71732021 ID=Msa1266740-mRNA-1:exon:24091;Parent=Msa1266740-mRNA-1
chr8_2 five_prime_UTR 71732021 71732143 71732021 ID=Msa1266740-mRNA-1:five_prime_utr;Parent=Msa1266740-mRNA-1
chr8_2 five_prime_UTR 71731560 71731634 71731560 ID=Msa1266740-mRNA-1:five_prime_utr;Parent=Msa1266740-mRNA-1
chr8_2 CDS 71731130 71731559 71731130 ID=Msa1266740-mRNA-1:cds;Parent=Msa1266740-mRNA-1
chr8_2 CDS 71730989 71731026 71730989 ID=Msa1266740-mRNA-1:cds;Parent=Msa1266740-mRNA-1
chr8_2 three_prime_UTR 71730931 71730988 71730931 ID=Msa1266740-mRNA-1:three_prime_utr;Parent=Msa1266740-mRNA-1
Gene Sequence

>Msa1266740

ATGGACGATGCTGGAGTCTCGAAATTAACTGCTAAATTGAAAGAAATATTTCAAAAGTGGCAAGTGGGTTATAAAGAGGGCAATATTGATCATTCTGGTGTGAATCATGGAGGAATTTCACCTATGATTAACAAAAGGTTAAACAGTTTAGTGTCTTTTGATTCTGATGAAGATAGTTGCAATAGTCCTAAAGCACCGCACGATGTTCCCAAAGGATACTTAGCTGTCTATGTTGGTCCTGAGCTTAGGAGATTCATCATTCCCACAAGCTACCTTAGCCACTCACTTTTCAAAATGTTGTTGGAAAAAGCTGCTGATGAATTTGGTTTTAATCAGTGTGGTGGACTTACAATTCCATGTGAAATTGAAACCTTTAAATACCTTCTCAGCTGTATGGAGAATACTCAGCTTCATCATGATCAAACCTCTTCTGGAAACACAGGAACTGTGGAAGAGAGAATAAACTAG
Protein sequence

>Msa1266740

MDDAGVSKLTAKLKEIFQKWQVGYKEGNIDHSGVNHGGISPMINKRLNSLVSFDSDEDSCNSPKAPHDVPKGYLAVYVGPELRRFIIPTSYLSHSLFKMLLEKAADEFGFNQCGGLTIPCEIETFKYLLSCMENTQLHHDQTSSGNTGTVEERIN*