AlfalfaGEDB | Msa1227800

M. sativa cultivar zhongmu-4 / Msa1227800

KEGG: -
GO: GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141
PFAM: PF03106.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1227800	XP_003626678.1	80.731	301	33	5	5	304	3	279	5.51e-159	454

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1227800	sp\|Q9LY00\|WRK70_ARATH	39.227	181	86	6	6	172	10	180	2.32e-32	124

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification
Msa1227800	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1227800	Msa1270910	0.913711	4.480761e-84	-8.615850e-47
Msa1227800	Msa1369340	0.883138	5.782803e-71	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1227800	MtrunA17_Chr8g0334541	80.731	301	33	5	5	304	3	279	5.06e-163	454
Msa1227800	MtrunA17_Chr8g0350621	49.430	263	105	8	1	255	1	243	5.54e-72	223
Msa1227800	MtrunA17_Chr7g0244971	44.251	287	145	5	4	287	12	286	6.62e-65	206
Msa1227800	MtrunA17_Chr7g0244981	40.956	293	136	8	4	287	11	275	5.41e-62	198
Msa1227800	MtrunA17_Chr2g0315371	40.444	225	98	7	9	205	18	234	4.50e-44	152
Msa1227800	MtrunA17_Chr2g0315381	38.645	251	107	8	2	214	10	251	8.53e-39	139
Msa1227800	MtrunA17_Chr2g0315361	40.329	243	103	8	6	213	14	249	1.90e-37	135
Msa1227800	MtrunA17_Chr8g0350631	49.419	172	58	6	133	302	5	149	3.44e-37	130
Msa1227800	MtrunA17_Chr3g0128671	45.833	168	78	6	13	171	23	186	1.99e-33	124
Msa1227800	MtrunA17_Chr5g0428351	40.606	165	85	3	8	172	92	243	1.85e-32	122
Msa1227800	MtrunA17_Chr7g0275531	37.500	176	95	5	4	171	9	177	5.00e-27	107
Msa1227800	MtrunA17_Chr8g0350641	45.588	136	62	4	6	132	7	139	2.37e-25	98.6
Msa1227800	MtrunA17_Chr3g0126391	30.392	204	111	4	1	178	1	199	8.82e-23	96.7
Msa1227800	MtrunA17_Chr8g0387981	31.771	192	104	4	8	178	13	198	1.12e-21	93.6
Msa1227800	MtrunA17_Chr8g0347251	47.674	86	38	1	93	171	110	195	8.31e-20	88.2
Msa1227800	MtrunA17_Chr7g0224801	54.237	59	24	1	112	170	93	148	1.07e-14	70.9
Msa1227800	MtrunA17_Chr8g0383351	47.458	59	28	1	112	170	160	215	1.04e-13	69.3
Msa1227800	MtrunA17_Chr7g0238921	56.897	58	22	1	112	169	124	178	4.18e-13	68.6
Msa1227800	MtrunA17_Chr3g0129361	47.458	59	28	1	112	170	144	199	4.93e-13	67.0
Msa1227800	MtrunA17_Chr7g0248721	52.308	65	25	3	112	174	141	201	8.60e-13	66.6
Msa1227800	MtrunA17_Chr5g0418781	50.000	58	26	1	112	169	124	178	1.88e-12	66.2
Msa1227800	MtrunA17_Chr8g0335811	50.000	58	26	1	112	169	154	208	2.90e-12	65.1
Msa1227800	MtrunA17_Chr4g0051881	51.724	58	25	1	112	169	178	232	2.94e-12	66.2
Msa1227800	MtrunA17_Chr8g0348311	43.590	78	40	2	95	170	136	211	2.99e-12	66.2
Msa1227800	MtrunA17_Chr5g0431811	37.500	96	53	3	110	202	670	761	3.23e-12	67.0
Msa1227800	MtrunA17_Chr5g0443131	50.000	58	26	1	112	169	157	211	3.39e-12	65.9
Msa1227800	MtrunA17_Chr8g0386241	36.782	87	50	2	84	170	77	158	8.38e-12	62.8
Msa1227800	MtrunA17_Chr1g0194481	47.458	59	28	1	112	170	158	213	2.19e-11	63.5
Msa1227800	MtrunA17_Chr2g0294231	47.458	59	28	1	112	170	203	258	3.18e-11	63.5
Msa1227800	MtrunA17_Chr2g0315401	52.542	59	27	1	140	198	19	76	4.43e-11	60.1
Msa1227800	MtrunA17_Chr4g0068541	44.828	58	29	1	112	169	208	262	6.72e-11	62.8
Msa1227800	MtrunA17_Chr7g0270191	47.458	59	28	1	112	170	145	200	6.82e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1227800	AT3G56400.1	39.227	181	86	6	6	172	10	180	2.36e-33	124
Msa1227800	AT2G46400.1	34.896	192	113	4	9	200	6	185	2.19e-26	105
Msa1227800	AT1G66550.1	32.039	206	110	6	6	203	8	191	7.49e-26	103
Msa1227800	AT1G66550.2	31.579	209	102	5	6	203	8	186	7.18e-25	100
Msa1227800	AT1G66600.1	33.333	195	109	4	6	199	8	182	7.24e-25	100
Msa1227800	AT5G22570.1	32.178	202	117	5	5	197	8	198	9.46e-25	101
Msa1227800	AT1G66560.1	35.533	197	100	7	7	199	9	182	3.13e-24	99.0
Msa1227800	AT4G11070.1	30.583	206	98	4	3	173	7	202	2.41e-22	95.1
Msa1227800	AT2G40750.1	29.915	234	128	6	6	207	14	243	1.58e-21	93.6
Msa1227800	AT5G01900.1	35.838	173	98	5	4	171	5	169	4.87e-21	90.9
Msa1227800	AT2G40740.1	35.256	156	57	4	89	231	137	261	8.69e-20	87.8
Msa1227800	AT2G40740.3	35.256	156	57	4	89	231	146	270	1.16e-19	87.8
Msa1227800	AT4G11070.2	31.351	185	92	3	3	173	7	170	1.43e-19	87.0
Msa1227800	AT5G24110.1	31.461	178	105	4	4	177	14	178	1.82e-19	87.0
Msa1227800	AT1G80590.1	31.928	166	84	4	6	171	8	144	1.01e-18	84.0
Msa1227800	AT4G23810.1	46.591	88	40	1	93	173	132	219	1.96e-18	84.3
Msa1227800	AT1G64000.1	49.231	65	28	2	112	174	114	175	1.02e-13	68.9
Msa1227800	AT2G46130.1	49.231	65	28	2	112	174	30	91	1.25e-13	66.6
Msa1227800	AT5G45260.1	51.515	66	30	1	106	169	1200	1265	1.72e-13	71.6
Msa1227800	AT5G41570.1	47.692	65	29	2	112	174	98	159	7.46e-13	66.2
Msa1227800	AT1G69310.4	50.000	58	26	1	112	169	147	201	7.58e-13	68.2
Msa1227800	AT1G69310.2	50.000	58	26	1	112	169	147	201	7.58e-13	68.2
Msa1227800	AT1G69310.1	50.000	58	26	1	112	169	147	201	7.58e-13	68.2
Msa1227800	AT5G49520.1	50.847	59	26	1	112	170	221	276	1.26e-12	68.2
Msa1227800	AT2G44745.1	50.000	58	26	1	112	169	145	199	2.03e-12	65.9
Msa1227800	AT4G39410.1	42.500	80	35	3	96	169	203	277	2.03e-12	67.0
Msa1227800	AT4G18170.1	41.667	96	49	3	76	169	136	226	4.36e-12	66.2
Msa1227800	AT1G29860.1	50.000	58	26	1	112	169	136	190	4.68e-12	65.9
Msa1227800	AT5G13080.1	49.153	59	27	1	112	170	67	122	8.07e-12	62.4
Msa1227800	AT4G01250.1	31.061	132	88	2	38	169	55	183	1.56e-11	64.3
Msa1227800	AT5G46350.1	48.276	58	27	1	112	169	183	237	2.34e-11	63.9
Msa1227800	AT3G01970.1	47.458	59	28	1	112	170	65	120	7.10e-11	60.1

Find 76 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATATACACACCTTGAGTATT+TGG	0.203884	8_2:-3192625	None:intergenic
AAAACGAGTAACCTCATTAT+TGG	0.242421	8_2:+3193360	Msa1227800:three_prime_UTR
GAAATAGAGTTATCTAAAAT+TGG	0.244498	8_2:-3193130	None:intergenic
TGATGTCGACGATGATAAAA+AGG	0.250505	8_2:-3193014	None:intergenic
ACAAGATGTTGTGCAAATAA+TGG	0.269160	8_2:-3192057	None:intergenic
AAGTGAGAGAGTTGAAAATA+TGG	0.295847	8_2:+3191930	Msa1227800:exon
TTCAATTCTCTGCACTTGTT+TGG	0.311204	8_2:-3192831	None:intergenic
CAAGATGTTGTGCAAATAAT+GGG	0.321057	8_2:-3192056	None:intergenic
CTCATGTACAATATACAAAA+TGG	0.346875	8_2:-3193326	None:intergenic
CAAGGAGAACTCACCTTCCT+TGG	0.368416	8_2:+3193084	Msa1227800:CDS
TTTGATGATGTTTGTTCAAA+AGG	0.368943	8_2:-3192116	None:intergenic
ACAAGTGTGGTGGCCGTAAT+AGG	0.383562	8_2:-3192879	None:intergenic
ACCTTGAGTATTTGGAATTG+AGG	0.393271	8_2:-3192617	None:intergenic
GAGTTATCTAAAATTGGTTC+AGG	0.405651	8_2:-3193124	None:intergenic
TATAATTGAAAGCCATGAAA+AGG	0.416194	8_2:-3193280	None:intergenic
CAATCTTGTTCGGTTGGAGT+TGG	0.418906	8_2:-3192989	None:intergenic
AAACGAGTAACCTCATTATT+GGG	0.421058	8_2:+3193361	Msa1227800:three_prime_UTR
CAACAGATGATGGGCATGAA+TGG	0.421200	8_2:+3192571	Msa1227800:CDS
TTGTATAAGACCACCTATTA+CGG	0.432341	8_2:+3192866	Msa1227800:CDS
AACACACATACAAGAGCATT+AGG	0.440774	8_2:+3191808	Msa1227800:five_prime_UTR
GTTGTTAAAGCTAAGCAATA+TGG	0.446307	8_2:-3192960	None:intergenic
GGGCATGAATGGAGAAAGTA+TGG	0.453906	8_2:+3192582	Msa1227800:CDS
GCATTAGGAAGATGGAAGGA+AGG	0.455060	8_2:+3191823	Msa1227800:five_prime_UTR
TATAATGATATATGAGGTAA+TGG	0.457382	8_2:+3193409	Msa1227800:three_prime_UTR
TCAGAAAGACCAACAGATGA+TGG	0.460980	8_2:+3192561	Msa1227800:CDS
AATCTTGTTCGGTTGGAGTT+GGG	0.465300	8_2:-3192988	None:intergenic
CAAAATGGCCGGACATGTAA+AGG	0.471506	8_2:-3193311	None:intergenic
TGGTAATATAATGATATATG+AGG	0.479365	8_2:+3193403	Msa1227800:three_prime_UTR
CACTTGAATCTGATGATCAC+AGG	0.481025	8_2:+3193191	Msa1227800:CDS
TTTGAACAAACATCATCAAA+GGG	0.495098	8_2:+3192119	Msa1227800:CDS
TTTAACTATTAAATCTGAAG+AGG	0.496351	8_2:+3192155	Msa1227800:CDS
CATTCATGCCCATCATCTGT+TGG	0.504398	8_2:-3192570	None:intergenic
TGATAGAGATGAGGCAATGA+AGG	0.505201	8_2:+3191954	Msa1227800:CDS
AAGGGACAATCTTGTTCGGT+TGG	0.505479	8_2:-3192995	None:intergenic
AGTATTATCATGAGGAGAAG+TGG	0.509303	8_2:-3192930	None:intergenic
GTAGATAACCTTTACATGTC+CGG	0.510188	8_2:+3193303	Msa1227800:exon
ATAATTGAAAGCCATGAAAA+GGG	0.510799	8_2:-3193279	None:intergenic
GATATGATGATAATATTGTA+TGG	0.516217	8_2:+3193214	Msa1227800:CDS
CATTAGGAAGATGGAAGGAA+GGG	0.523179	8_2:+3191824	Msa1227800:five_prime_UTR
ATACAAGAGCATTAGGAAGA+TGG	0.532214	8_2:+3191815	Msa1227800:five_prime_UTR
CTAGAGGATCTCACAAAAGA+AGG	0.538260	8_2:+3192217	Msa1227800:CDS
GATGTTTGTTCAAAAGGAAG+AGG	0.548943	8_2:-3192110	None:intergenic
GTGAGATCCTCTAGCACCAC+GGG	0.549156	8_2:-3192208	None:intergenic
ATATATGCAGGGACTACTAC+AGG	0.552760	8_2:+3192774	Msa1227800:intron
TAGATAACTCTATTTCTACA+AGG	0.554534	8_2:+3193137	Msa1227800:CDS
TAATAGGTGGTCTTATACAA+GGG	0.559513	8_2:-3192863	None:intergenic
TCCTCAATTCCAAATACTCA+AGG	0.566315	8_2:+3192616	Msa1227800:CDS
GTATTATCATGAGGAGAAGT+GGG	0.567061	8_2:-3192929	None:intergenic
TTGTTCGGTTGGAGTTGGGA+AGG	0.567594	8_2:-3192984	None:intergenic
CAGAAAGACCAACAGATGAT+GGG	0.573763	8_2:+3192562	Msa1227800:CDS
ATGTTTGTTCAAAAGGAAGA+GGG	0.576811	8_2:-3192109	None:intergenic
GTAATAGGTGGTCTTATACA+AGG	0.578341	8_2:-3192864	None:intergenic
AGTTCCACCCGTGGTGCTAG+AGG	0.580528	8_2:+3192201	Msa1227800:CDS
AGAGAGCAGGTATTCATCCA+AGG	0.581141	8_2:-3193101	None:intergenic
GAAAGCCATGAAAAGGGTGA+AGG	0.584427	8_2:-3193273	None:intergenic
CATGCATTGTCCCAATAATG+AGG	0.585488	8_2:-3193371	None:intergenic
AAGAGCATTAGGAAGATGGA+AGG	0.586002	8_2:+3191819	Msa1227800:five_prime_UTR
GACAATGCATGTGATGACTT+TGG	0.586647	8_2:+3193383	Msa1227800:three_prime_UTR
TGTACAATATACAAAATGGC+CGG	0.591384	8_2:-3193322	None:intergenic
GCAATATTCCACTGAATGTG+AGG	0.603090	8_2:-3193062	None:intergenic
AGCAGGTATTCATCCAAGGA+AGG	0.605278	8_2:-3193097	None:intergenic
ACATTCAGTGGAATATTGCA+AGG	0.616091	8_2:+3193066	Msa1227800:CDS
GTTAAAGCTAAGCAATATGG+AGG	0.618068	8_2:-3192957	None:intergenic
GAGGAAGTAGTATTATCATG+AGG	0.627255	8_2:-3192938	None:intergenic
AAATTGGTTCAGGAGAGAGC+AGG	0.628355	8_2:-3193114	None:intergenic
GCAATGAAGGAGCTTGTGAG+AGG	0.633274	8_2:+3191967	Msa1227800:CDS
CAATGAAGGAGCTTGTGAGA+GGG	0.635141	8_2:+3191968	Msa1227800:CDS
CTAATTTGTTACAAGTGTGG+TGG	0.636211	8_2:-3192889	None:intergenic
TGTGAGATCCTCTAGCACCA+CGG	0.642464	8_2:-3192209	None:intergenic
AGATCCTCTAGCACCACGGG+TGG	0.645633	8_2:-3192205	None:intergenic
AGTGTGGTGGCCGTAATAGG+TGG	0.645916	8_2:-3192876	None:intergenic
AAATATGGATGATAGAGATG+AGG	0.650081	8_2:+3191945	Msa1227800:exon
ACTTGAATCTGATGATCACA+GGG	0.651483	8_2:+3193192	Msa1227800:CDS
TGTCTAATTTGTTACAAGTG+TGG	0.656286	8_2:-3192892	None:intergenic
TAGGTGGTCTTATACAAGGG+AGG	0.682870	8_2:-3192860	None:intergenic
TACAAGACTAGTTCCACCCG+TGG	0.693459	8_2:+3192192	Msa1227800:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATATATATTAATAATATAT+TGG	-	chr8_2:3192289-3192308	None:intergenic	0.0%
!!	ATATATTATTAATATATATA+TGG	+	chr8_2:3192289-3192308	Msa1227800:intron	0.0%
!!	TATATATATTAATAATATAT+TGG	-	chr8_2:3192289-3192308	None:intergenic	0.0%
!!	ATATATTATTAATATATATA+TGG	+	chr8_2:3192289-3192308	Msa1227800:intron	0.0%
!!	TATATATTAATTCAATTCAT+TGG	-	chr8_2:3192759-3192778	None:intergenic	10.0%
!!	TATATATTAATTCAATTCAT+TGG	-	chr8_2:3192759-3192778	None:intergenic	10.0%
!!	TATATATAGATGAATTAAGT+TGG	-	chr8_2:3192675-3192694	None:intergenic	15.0%
!!	AATTGAATTAATATATATGC+AGG	+	chr8_2:3192762-3192781	Msa1227800:intron	15.0%
!!	ATTGAATTAATATATATGCA+GGG	+	chr8_2:3192763-3192782	Msa1227800:intron	15.0%
!!	TATATATAGATGAATTAAGT+TGG	-	chr8_2:3192675-3192694	None:intergenic	15.0%
!!	AATTGAATTAATATATATGC+AGG	+	chr8_2:3192762-3192781	Msa1227800:intron	15.0%
!!	ATTGAATTAATATATATGCA+GGG	+	chr8_2:3192763-3192782	Msa1227800:intron	15.0%
!!	TTTAACTATTAAATCTGAAG+AGG	+	chr8_2:3192155-3192174	Msa1227800:CDS	20.0%
!!!	GAAATAGAGTTATCTAAAAT+TGG	-	chr8_2:3193133-3193152	None:intergenic	20.0%
!!	GATATGATGATAATATTGTA+TGG	+	chr8_2:3193214-3193233	Msa1227800:CDS	20.0%
!!	TGGTAATATAATGATATATG+AGG	+	chr8_2:3193403-3193422	Msa1227800:three_prime_UTR	20.0%
!!	TATAATGATATATGAGGTAA+TGG	+	chr8_2:3193409-3193428	Msa1227800:three_prime_UTR	20.0%
!!	TTTAACTATTAAATCTGAAG+AGG	+	chr8_2:3192155-3192174	Msa1227800:CDS	20.0%
!!!	GAAATAGAGTTATCTAAAAT+TGG	-	chr8_2:3193133-3193152	None:intergenic	20.0%
!!	GATATGATGATAATATTGTA+TGG	+	chr8_2:3193214-3193233	Msa1227800:CDS	20.0%
!!	TGGTAATATAATGATATATG+AGG	+	chr8_2:3193403-3193422	Msa1227800:three_prime_UTR	20.0%
!!	TATAATGATATATGAGGTAA+TGG	+	chr8_2:3193409-3193428	Msa1227800:three_prime_UTR	20.0%
!!!	TTTGATGATGTTTGTTCAAA+AGG	-	chr8_2:3192119-3192138	None:intergenic	25.0%
!!	TTTTGAACAAACATCATCAA+AGG	+	chr8_2:3192118-3192137	Msa1227800:CDS	25.0%
!	TTTGAACAAACATCATCAAA+GGG	+	chr8_2:3192119-3192138	Msa1227800:CDS	25.0%
!!!	GTTTTTAAAGAGTGTTAACA+AGG	-	chr8_2:3192395-3192414	None:intergenic	25.0%
!!	GAACATTTTCTATAGAATCA+TGG	+	chr8_2:3192443-3192462	Msa1227800:intron	25.0%
!!	TAGAATCATGGTTAAGTTTT+TGG	+	chr8_2:3192455-3192474	Msa1227800:intron	25.0%
!!!	TAGATAACTCTATTTCTACA+AGG	+	chr8_2:3193137-3193156	Msa1227800:CDS	25.0%
!	ATAATTGAAAGCCATGAAAA+GGG	-	chr8_2:3193282-3193301	None:intergenic	25.0%
!	TATAATTGAAAGCCATGAAA+AGG	-	chr8_2:3193283-3193302	None:intergenic	25.0%
!	CTCATGTACAATATACAAAA+TGG	-	chr8_2:3193329-3193348	None:intergenic	25.0%
!!!	TTTGATGATGTTTGTTCAAA+AGG	-	chr8_2:3192119-3192138	None:intergenic	25.0%
!!	TTTTGAACAAACATCATCAA+AGG	+	chr8_2:3192118-3192137	Msa1227800:CDS	25.0%
!	TTTGAACAAACATCATCAAA+GGG	+	chr8_2:3192119-3192138	Msa1227800:CDS	25.0%
!!!	GTTTTTAAAGAGTGTTAACA+AGG	-	chr8_2:3192395-3192414	None:intergenic	25.0%
!!	GAACATTTTCTATAGAATCA+TGG	+	chr8_2:3192443-3192462	Msa1227800:intron	25.0%
!!	TAGAATCATGGTTAAGTTTT+TGG	+	chr8_2:3192455-3192474	Msa1227800:intron	25.0%
!!!	TAGATAACTCTATTTCTACA+AGG	+	chr8_2:3193137-3193156	Msa1227800:CDS	25.0%
!	ATAATTGAAAGCCATGAAAA+GGG	-	chr8_2:3193282-3193301	None:intergenic	25.0%
!	TATAATTGAAAGCCATGAAA+AGG	-	chr8_2:3193283-3193302	None:intergenic	25.0%
!	CTCATGTACAATATACAAAA+TGG	-	chr8_2:3193329-3193348	None:intergenic	25.0%
	AAGTGAGAGAGTTGAAAATA+TGG	+	chr8_2:3191930-3191949	Msa1227800:exon	30.0%
	AAATATGGATGATAGAGATG+AGG	+	chr8_2:3191945-3191964	Msa1227800:exon	30.0%
	CAAGATGTTGTGCAAATAAT+GGG	-	chr8_2:3192059-3192078	None:intergenic	30.0%
	ACAAGATGTTGTGCAAATAA+TGG	-	chr8_2:3192060-3192079	None:intergenic	30.0%
	ATGTTTGTTCAAAAGGAAGA+GGG	-	chr8_2:3192112-3192131	None:intergenic	30.0%
	AACATGAAATTCTCATTGTG+TGG	+	chr8_2:3192361-3192380	Msa1227800:intron	30.0%
	ATATACACACCTTGAGTATT+TGG	-	chr8_2:3192628-3192647	None:intergenic	30.0%
!!	TAATAGGTGGTCTTATACAA+GGG	-	chr8_2:3192866-3192885	None:intergenic	30.0%
	TTGTATAAGACCACCTATTA+CGG	+	chr8_2:3192866-3192885	Msa1227800:CDS	30.0%
!!	TGTCTAATTTGTTACAAGTG+TGG	-	chr8_2:3192895-3192914	None:intergenic	30.0%
	GTTGTTAAAGCTAAGCAATA+TGG	-	chr8_2:3192963-3192982	None:intergenic	30.0%
	TAAAAAGGGACAATCTTGTT+CGG	-	chr8_2:3193002-3193021	None:intergenic	30.0%
!!	TTTTAATTCCTCACATTCAG+TGG	+	chr8_2:3193054-3193073	Msa1227800:CDS	30.0%
!	GAGTTATCTAAAATTGGTTC+AGG	-	chr8_2:3193127-3193146	None:intergenic	30.0%
	TGTACAATATACAAAATGGC+CGG	-	chr8_2:3193325-3193344	None:intergenic	30.0%
	AAAACGAGTAACCTCATTAT+TGG	+	chr8_2:3193360-3193379	Msa1227800:three_prime_UTR	30.0%
	AAACGAGTAACCTCATTATT+GGG	+	chr8_2:3193361-3193380	Msa1227800:three_prime_UTR	30.0%
	AAGTGAGAGAGTTGAAAATA+TGG	+	chr8_2:3191930-3191949	Msa1227800:exon	30.0%
	AAATATGGATGATAGAGATG+AGG	+	chr8_2:3191945-3191964	Msa1227800:exon	30.0%
	CAAGATGTTGTGCAAATAAT+GGG	-	chr8_2:3192059-3192078	None:intergenic	30.0%
	ACAAGATGTTGTGCAAATAA+TGG	-	chr8_2:3192060-3192079	None:intergenic	30.0%
	ATGTTTGTTCAAAAGGAAGA+GGG	-	chr8_2:3192112-3192131	None:intergenic	30.0%
	AACATGAAATTCTCATTGTG+TGG	+	chr8_2:3192361-3192380	Msa1227800:intron	30.0%
	ATATACACACCTTGAGTATT+TGG	-	chr8_2:3192628-3192647	None:intergenic	30.0%
!!	TAATAGGTGGTCTTATACAA+GGG	-	chr8_2:3192866-3192885	None:intergenic	30.0%
	TTGTATAAGACCACCTATTA+CGG	+	chr8_2:3192866-3192885	Msa1227800:CDS	30.0%
!!	TGTCTAATTTGTTACAAGTG+TGG	-	chr8_2:3192895-3192914	None:intergenic	30.0%
	GTTGTTAAAGCTAAGCAATA+TGG	-	chr8_2:3192963-3192982	None:intergenic	30.0%
	TAAAAAGGGACAATCTTGTT+CGG	-	chr8_2:3193002-3193021	None:intergenic	30.0%
!!	TTTTAATTCCTCACATTCAG+TGG	+	chr8_2:3193054-3193073	Msa1227800:CDS	30.0%
!	GAGTTATCTAAAATTGGTTC+AGG	-	chr8_2:3193127-3193146	None:intergenic	30.0%
	TGTACAATATACAAAATGGC+CGG	-	chr8_2:3193325-3193344	None:intergenic	30.0%
	AAAACGAGTAACCTCATTAT+TGG	+	chr8_2:3193360-3193379	Msa1227800:three_prime_UTR	30.0%
	AAACGAGTAACCTCATTATT+GGG	+	chr8_2:3193361-3193380	Msa1227800:three_prime_UTR	30.0%
!!	AACACACATACAAGAGCATT+AGG	+	chr8_2:3191808-3191827	Msa1227800:five_prime_UTR	35.0%
!!	ATACAAGAGCATTAGGAAGA+TGG	+	chr8_2:3191815-3191834	Msa1227800:five_prime_UTR	35.0%
	GATGTTTGTTCAAAAGGAAG+AGG	-	chr8_2:3192113-3192132	None:intergenic	35.0%
!!	TCATGGTTAAGTTTTTGGAG+TGG	+	chr8_2:3192460-3192479	Msa1227800:intron	35.0%
	AAAAACTACAGAAACGTGTG+AGG	+	chr8_2:3192533-3192552	Msa1227800:CDS	35.0%
	TCCTCAATTCCAAATACTCA+AGG	+	chr8_2:3192616-3192635	Msa1227800:CDS	35.0%
!	ACCTTGAGTATTTGGAATTG+AGG	-	chr8_2:3192620-3192639	None:intergenic	35.0%
	TTCAATTCTCTGCACTTGTT+TGG	-	chr8_2:3192834-3192853	None:intergenic	35.0%
!!	GTAATAGGTGGTCTTATACA+AGG	-	chr8_2:3192867-3192886	None:intergenic	35.0%
!!	CTAATTTGTTACAAGTGTGG+TGG	-	chr8_2:3192892-3192911	None:intergenic	35.0%
	GTATTATCATGAGGAGAAGT+GGG	-	chr8_2:3192932-3192951	None:intergenic	35.0%
	AGTATTATCATGAGGAGAAG+TGG	-	chr8_2:3192933-3192952	None:intergenic	35.0%
	GAGGAAGTAGTATTATCATG+AGG	-	chr8_2:3192941-3192960	None:intergenic	35.0%
	GTTAAAGCTAAGCAATATGG+AGG	-	chr8_2:3192960-3192979	None:intergenic	35.0%
	GATGTCGACGATGATAAAAA+GGG	-	chr8_2:3193016-3193035	None:intergenic	35.0%
	TGATGTCGACGATGATAAAA+AGG	-	chr8_2:3193017-3193036	None:intergenic	35.0%
	ACATTCAGTGGAATATTGCA+AGG	+	chr8_2:3193066-3193085	Msa1227800:CDS	35.0%
	ACTTGAATCTGATGATCACA+GGG	+	chr8_2:3193192-3193211	Msa1227800:CDS	35.0%
	GTAGATAACCTTTACATGTC+CGG	+	chr8_2:3193303-3193322	Msa1227800:exon	35.0%
!	TGAGGTAATGGTAACAGAAA+AGG	+	chr8_2:3193421-3193440	Msa1227800:three_prime_UTR	35.0%
!!	AACACACATACAAGAGCATT+AGG	+	chr8_2:3191808-3191827	Msa1227800:five_prime_UTR	35.0%
!!	ATACAAGAGCATTAGGAAGA+TGG	+	chr8_2:3191815-3191834	Msa1227800:five_prime_UTR	35.0%
	GATGTTTGTTCAAAAGGAAG+AGG	-	chr8_2:3192113-3192132	None:intergenic	35.0%
!!	TCATGGTTAAGTTTTTGGAG+TGG	+	chr8_2:3192460-3192479	Msa1227800:intron	35.0%
	AAAAACTACAGAAACGTGTG+AGG	+	chr8_2:3192533-3192552	Msa1227800:CDS	35.0%
	TCCTCAATTCCAAATACTCA+AGG	+	chr8_2:3192616-3192635	Msa1227800:CDS	35.0%
!	ACCTTGAGTATTTGGAATTG+AGG	-	chr8_2:3192620-3192639	None:intergenic	35.0%
	TTCAATTCTCTGCACTTGTT+TGG	-	chr8_2:3192834-3192853	None:intergenic	35.0%
!!	GTAATAGGTGGTCTTATACA+AGG	-	chr8_2:3192867-3192886	None:intergenic	35.0%
!!	CTAATTTGTTACAAGTGTGG+TGG	-	chr8_2:3192892-3192911	None:intergenic	35.0%
	GTATTATCATGAGGAGAAGT+GGG	-	chr8_2:3192932-3192951	None:intergenic	35.0%
	AGTATTATCATGAGGAGAAG+TGG	-	chr8_2:3192933-3192952	None:intergenic	35.0%
	GAGGAAGTAGTATTATCATG+AGG	-	chr8_2:3192941-3192960	None:intergenic	35.0%
	GTTAAAGCTAAGCAATATGG+AGG	-	chr8_2:3192960-3192979	None:intergenic	35.0%
	GATGTCGACGATGATAAAAA+GGG	-	chr8_2:3193016-3193035	None:intergenic	35.0%
	TGATGTCGACGATGATAAAA+AGG	-	chr8_2:3193017-3193036	None:intergenic	35.0%
	ACATTCAGTGGAATATTGCA+AGG	+	chr8_2:3193066-3193085	Msa1227800:CDS	35.0%
	ACTTGAATCTGATGATCACA+GGG	+	chr8_2:3193192-3193211	Msa1227800:CDS	35.0%
	GTAGATAACCTTTACATGTC+CGG	+	chr8_2:3193303-3193322	Msa1227800:exon	35.0%
!!	AAGAGCATTAGGAAGATGGA+AGG	+	chr8_2:3191819-3191838	Msa1227800:five_prime_UTR	40.0%
	CATTAGGAAGATGGAAGGAA+GGG	+	chr8_2:3191824-3191843	Msa1227800:five_prime_UTR	40.0%
	TGATAGAGATGAGGCAATGA+AGG	+	chr8_2:3191954-3191973	Msa1227800:CDS	40.0%
	CTAGAGGATCTCACAAAAGA+AGG	+	chr8_2:3192217-3192236	Msa1227800:CDS	40.0%
!	AGTCATGTTGTTTGCTAGCT+AGG	+	chr8_2:3192415-3192434	Msa1227800:intron	40.0%
	TCAGAAAGACCAACAGATGA+TGG	+	chr8_2:3192561-3192580	Msa1227800:CDS	40.0%
	CAGAAAGACCAACAGATGAT+GGG	+	chr8_2:3192562-3192581	Msa1227800:CDS	40.0%
	ATATATGCAGGGACTACTAC+AGG	+	chr8_2:3192774-3192793	Msa1227800:intron	40.0%
	AATCTTGTTCGGTTGGAGTT+GGG	-	chr8_2:3192991-3193010	None:intergenic	40.0%
	GCAATATTCCACTGAATGTG+AGG	-	chr8_2:3193065-3193084	None:intergenic	40.0%
	CACTTGAATCTGATGATCAC+AGG	+	chr8_2:3193191-3193210	Msa1227800:CDS	40.0%
!	GATTTCCTTCACCCTTTTCA+TGG	+	chr8_2:3193268-3193287	Msa1227800:CDS	40.0%
	CATGCATTGTCCCAATAATG+AGG	-	chr8_2:3193374-3193393	None:intergenic	40.0%
	GACAATGCATGTGATGACTT+TGG	+	chr8_2:3193383-3193402	Msa1227800:three_prime_UTR	40.0%
!!	AAGAGCATTAGGAAGATGGA+AGG	+	chr8_2:3191819-3191838	Msa1227800:five_prime_UTR	40.0%
	CATTAGGAAGATGGAAGGAA+GGG	+	chr8_2:3191824-3191843	Msa1227800:five_prime_UTR	40.0%
	TGATAGAGATGAGGCAATGA+AGG	+	chr8_2:3191954-3191973	Msa1227800:CDS	40.0%
	CTAGAGGATCTCACAAAAGA+AGG	+	chr8_2:3192217-3192236	Msa1227800:CDS	40.0%
!	AGTCATGTTGTTTGCTAGCT+AGG	+	chr8_2:3192415-3192434	Msa1227800:intron	40.0%
	TCAGAAAGACCAACAGATGA+TGG	+	chr8_2:3192561-3192580	Msa1227800:CDS	40.0%
	CAGAAAGACCAACAGATGAT+GGG	+	chr8_2:3192562-3192581	Msa1227800:CDS	40.0%
	ATATATGCAGGGACTACTAC+AGG	+	chr8_2:3192774-3192793	Msa1227800:intron	40.0%
	AATCTTGTTCGGTTGGAGTT+GGG	-	chr8_2:3192991-3193010	None:intergenic	40.0%
	GCAATATTCCACTGAATGTG+AGG	-	chr8_2:3193065-3193084	None:intergenic	40.0%
	CACTTGAATCTGATGATCAC+AGG	+	chr8_2:3193191-3193210	Msa1227800:CDS	40.0%
!	GATTTCCTTCACCCTTTTCA+TGG	+	chr8_2:3193268-3193287	Msa1227800:CDS	40.0%
	CATGCATTGTCCCAATAATG+AGG	-	chr8_2:3193374-3193393	None:intergenic	40.0%
	GACAATGCATGTGATGACTT+TGG	+	chr8_2:3193383-3193402	Msa1227800:three_prime_UTR	40.0%
	GCATTAGGAAGATGGAAGGA+AGG	+	chr8_2:3191823-3191842	Msa1227800:five_prime_UTR	45.0%
	CAATGAAGGAGCTTGTGAGA+GGG	+	chr8_2:3191968-3191987	Msa1227800:CDS	45.0%
	CATTCATGCCCATCATCTGT+TGG	-	chr8_2:3192573-3192592	None:intergenic	45.0%
!!	CAACAGATGATGGGCATGAA+TGG	+	chr8_2:3192571-3192590	Msa1227800:CDS	45.0%
	GGGCATGAATGGAGAAAGTA+TGG	+	chr8_2:3192582-3192601	Msa1227800:CDS	45.0%
!!	TAGGTGGTCTTATACAAGGG+AGG	-	chr8_2:3192863-3192882	None:intergenic	45.0%
	CAATCTTGTTCGGTTGGAGT+TGG	-	chr8_2:3192992-3193011	None:intergenic	45.0%
	AAGGGACAATCTTGTTCGGT+TGG	-	chr8_2:3192998-3193017	None:intergenic	45.0%
	AGCAGGTATTCATCCAAGGA+AGG	-	chr8_2:3193100-3193119	None:intergenic	45.0%
	AGAGAGCAGGTATTCATCCA+AGG	-	chr8_2:3193104-3193123	None:intergenic	45.0%
!	AAATTGGTTCAGGAGAGAGC+AGG	-	chr8_2:3193117-3193136	None:intergenic	45.0%
	GAAAGCCATGAAAAGGGTGA+AGG	-	chr8_2:3193276-3193295	None:intergenic	45.0%
	CAAAATGGCCGGACATGTAA+AGG	-	chr8_2:3193314-3193333	None:intergenic	45.0%
	GCATTAGGAAGATGGAAGGA+AGG	+	chr8_2:3191823-3191842	Msa1227800:five_prime_UTR	45.0%
	CAATGAAGGAGCTTGTGAGA+GGG	+	chr8_2:3191968-3191987	Msa1227800:CDS	45.0%
	CATTCATGCCCATCATCTGT+TGG	-	chr8_2:3192573-3192592	None:intergenic	45.0%
!!	CAACAGATGATGGGCATGAA+TGG	+	chr8_2:3192571-3192590	Msa1227800:CDS	45.0%
	GGGCATGAATGGAGAAAGTA+TGG	+	chr8_2:3192582-3192601	Msa1227800:CDS	45.0%
!!	TAGGTGGTCTTATACAAGGG+AGG	-	chr8_2:3192863-3192882	None:intergenic	45.0%
	CAATCTTGTTCGGTTGGAGT+TGG	-	chr8_2:3192992-3193011	None:intergenic	45.0%
	AAGGGACAATCTTGTTCGGT+TGG	-	chr8_2:3192998-3193017	None:intergenic	45.0%
	AGCAGGTATTCATCCAAGGA+AGG	-	chr8_2:3193100-3193119	None:intergenic	45.0%
	AGAGAGCAGGTATTCATCCA+AGG	-	chr8_2:3193104-3193123	None:intergenic	45.0%
!	AAATTGGTTCAGGAGAGAGC+AGG	-	chr8_2:3193117-3193136	None:intergenic	45.0%
	GAAAGCCATGAAAAGGGTGA+AGG	-	chr8_2:3193276-3193295	None:intergenic	45.0%
	CAAAATGGCCGGACATGTAA+AGG	-	chr8_2:3193314-3193333	None:intergenic	45.0%
	GCAATGAAGGAGCTTGTGAG+AGG	+	chr8_2:3191967-3191986	Msa1227800:CDS	50.0%
!	TACAAGACTAGTTCCACCCG+TGG	+	chr8_2:3192192-3192211	Msa1227800:CDS	50.0%
	TGTGAGATCCTCTAGCACCA+CGG	-	chr8_2:3192212-3192231	None:intergenic	50.0%
	ACAAGTGTGGTGGCCGTAAT+AGG	-	chr8_2:3192882-3192901	None:intergenic	50.0%
	TTGTTCGGTTGGAGTTGGGA+AGG	-	chr8_2:3192987-3193006	None:intergenic	50.0%
	CAAGGAGAACTCACCTTCCT+TGG	+	chr8_2:3193084-3193103	Msa1227800:CDS	50.0%
	GCAATGAAGGAGCTTGTGAG+AGG	+	chr8_2:3191967-3191986	Msa1227800:CDS	50.0%
!	TACAAGACTAGTTCCACCCG+TGG	+	chr8_2:3192192-3192211	Msa1227800:CDS	50.0%
	TGTGAGATCCTCTAGCACCA+CGG	-	chr8_2:3192212-3192231	None:intergenic	50.0%
	ACAAGTGTGGTGGCCGTAAT+AGG	-	chr8_2:3192882-3192901	None:intergenic	50.0%
	TTGTTCGGTTGGAGTTGGGA+AGG	-	chr8_2:3192987-3193006	None:intergenic	50.0%
	CAAGGAGAACTCACCTTCCT+TGG	+	chr8_2:3193084-3193103	Msa1227800:CDS	50.0%
	GTGAGATCCTCTAGCACCAC+GGG	-	chr8_2:3192211-3192230	None:intergenic	55.0%
	AGTGTGGTGGCCGTAATAGG+TGG	-	chr8_2:3192879-3192898	None:intergenic	55.0%
	GTGAGATCCTCTAGCACCAC+GGG	-	chr8_2:3192211-3192230	None:intergenic	55.0%
	AGTGTGGTGGCCGTAATAGG+TGG	-	chr8_2:3192879-3192898	None:intergenic	55.0%
!	AGTTCCACCCGTGGTGCTAG+AGG	+	chr8_2:3192201-3192220	Msa1227800:CDS	60.0%
	AGATCCTCTAGCACCACGGG+TGG	-	chr8_2:3192208-3192227	None:intergenic	60.0%
!	AGTTCCACCCGTGGTGCTAG+AGG	+	chr8_2:3192201-3192220	Msa1227800:CDS	60.0%
	AGATCCTCTAGCACCACGGG+TGG	-	chr8_2:3192208-3192227	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	3191784	3193442	3191784	ID=Msa1227800;Name=Msa1227800
chr8_2	mRNA	3191784	3193442	3191784	ID=Msa1227800-mRNA-1;Parent=Msa1227800;Name=Msa1227800-mRNA-1;_AED=0.25;_eAED=0.25;_QI=165\|1\|1\|1\|1\|1\|3\|139\|304
chr8_2	exon	3191784	3192238	3191784	ID=Msa1227800-mRNA-1:exon:958;Parent=Msa1227800-mRNA-1
chr8_2	exon	3192533	3192637	3192533	ID=Msa1227800-mRNA-1:exon:959;Parent=Msa1227800-mRNA-1
chr8_2	exon	3192784	3193442	3192784	ID=Msa1227800-mRNA-1:exon:960;Parent=Msa1227800-mRNA-1
chr8_2	five_prime_UTR	3191784	3191948	3191784	ID=Msa1227800-mRNA-1:five_prime_utr;Parent=Msa1227800-mRNA-1
chr8_2	CDS	3191949	3192238	3191949	ID=Msa1227800-mRNA-1:cds;Parent=Msa1227800-mRNA-1
chr8_2	CDS	3192533	3192637	3192533	ID=Msa1227800-mRNA-1:cds;Parent=Msa1227800-mRNA-1
chr8_2	CDS	3192784	3193303	3192784	ID=Msa1227800-mRNA-1:cds;Parent=Msa1227800-mRNA-1
chr8_2	three_prime_UTR	3193304	3193442	3193304	ID=Msa1227800-mRNA-1:three_prime_utr;Parent=Msa1227800-mRNA-1

Gene Sequence

>Msa1227800

ATGGATGATAGAGATGAGGCAATGAAGGAGCTTGTGAGAGGGCGTGAGTTAGCTAAGCAGCTTGAACAACATCTTAGTAACAACATTAATGATAAATTATTAGCAAGCCCATTATTTGCACAACATCTTGTAAGCAACGTTGTCAACTCATTTAGTAACACCCTCTTCCTTTTGAACAAACATCATCAAAGGGAACAAAACTTTTCTTTAACTATTAAATCTGAAGAGGAATCTCAAGATAGTTACAAGACTAGTTCCACCCGTGGTGCTAGAGGATCTCACAAAAGAAGAAAAACTACAGAAACGTGTGAGGAAGTGTCAGAAAGACCAACAGATGATGGGCATGAATGGAGAAAGTATGGTCAAAAAACAATCCTCAATTCCAAATACTCAAGGGACTACTACAGGTGTACTCACAAGTTTAATCAAAGATGCAAAGCAACCAAACAAGTGCAGAGAATTGAAGAGAAGCCTCCCTTGTATAAGACCACCTATTACGGCCACCACACTTGTAACAAATTAGACAGTATCATATTTGAACCCACTTCTCCTCATGATAATACTACTTCCTCCATATTGCTTAGCTTTAACAACACCTTCCCAACTCCAACCGAACAAGATTGTCCCTTTTTATCATCGTCGACATCATCATCATCATCTTCGTCTTTTAATTCCTCACATTCAGTGGAATATTGCAAGGAGAACTCACCTTCCTTGGATGAATACCTGCTCTCTCCTGAACCAATTTTAGATAACTCTATTTCTACAAGGCATGTTCGTAGTACTCTATCATCATCAACTACACTTGAATCTGATGATCACAGGGATATGATGATAATATTGTATGGTCTCTTGTATGATTCTGATGAACTTGATACTGATTTCCTTCACCCTTTTCATGGCTTTCAATTATAG

Protein sequence

>Msa1227800

MDDRDEAMKELVRGRELAKQLEQHLSNNINDKLLASPLFAQHLVSNVVNSFSNTLFLLNKHHQREQNFSLTIKSEEESQDSYKTSSTRGARGSHKRRKTTETCEEVSERPTDDGHEWRKYGQKTILNSKYSRDYYRCTHKFNQRCKATKQVQRIEEKPPLYKTTYYGHHTCNKLDSIIFEPTSPHDNTTSSILLSFNNTFPTPTEQDCPFLSSSTSSSSSSSFNSSHSVEYCKENSPSLDEYLLSPEPILDNSISTRHVRSTLSSSTTLESDDHRDMMIILYGLLYDSDELDTDFLHPFHGFQL*