AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1229720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1229720 MtrunA17_Chr8g0337261 85.882 170 4 3 1 168 1 152 1.61e-55 172
Msa1229720 MtrunA17_Chr8g0337251 64.286 168 23 8 1 168 1 131 1.11e-31 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 82 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCCCTCAACTTTATTT+AGG 0.238868 8_2:+5655413 Msa1229720:three_prime_UTR
AAGTTTAAGCGTGGAAAATT+TGG 0.266461 8_2:+5655184 Msa1229720:CDS
CAACTTTATTTAGGTCTGTT+TGG 0.271608 8_2:+5655422 Msa1229720:three_prime_UTR
ATGAACAATTATTGTTCATT+TGG 0.293223 8_2:+5655345 Msa1229720:three_prime_UTR
AAGTATAAGCATGGAAAATT+TGG 0.307873 8_2:+5655127 Msa1229720:CDS
TATCATCATGGTTATGGTTA+TGG 0.313342 8_2:+5655097 Msa1229720:CDS
GTTCCAACAAGCATTCAATT+TGG 0.331950 8_2:+5653814 None:intergenic
AAGCATGGTAGGTTTGGTTT+TGG 0.332336 8_2:+5655160 Msa1229720:CDS
GCATTCAATTTGGTCACCTT+TGG 0.343406 8_2:+5653824 Msa1229720:five_prime_UTR
TCTTATCATGCTCCACAATA+TGG 0.348923 8_2:+5654453 Msa1229720:CDS
TATGGCTATCCATATCCTTC+TGG 0.365272 8_2:+5654471 Msa1229720:CDS
GGAGGTTATCATCATGGTTA+TGG 0.376411 8_2:+5655091 Msa1229720:CDS
CGTTGGAAGCATGGTAGGTT+TGG 0.389621 8_2:+5655154 Msa1229720:CDS
TCAAGTGTACCAGAAGGATA+TGG 0.413672 8_2:-5654480 None:intergenic
CCTTCCGGTTATCCTCATTC+CGG 0.421172 8_2:+5654414 Msa1229720:CDS
GAACCAGGTGGAGGAGGATA+AGG 0.429948 8_2:-5654279 None:intergenic
GGAGGATAAGGTGGTGCTGC+TGG 0.432218 8_2:-5654267 None:intergenic
TGTTATAAAGGGCGTGGATC+TGG 0.441402 8_2:+5655004 Msa1229720:intron
ATATCCGGAATGAGGATAAC+CGG 0.442176 8_2:-5654418 None:intergenic
GGTATAGGAGGATTAATTGC+AGG 0.442489 8_2:+5655025 Msa1229720:CDS
GCAAGCGATGGAAGTGATGC+AGG 0.445817 8_2:+5655206 Msa1229720:exon
AGGTTATTATCCACCGCAAC+CGG 0.446409 8_2:+5654323 Msa1229720:CDS
GGAGGAGGGTATCCACCTTC+CGG 0.452585 8_2:+5654399 Msa1229720:CDS
GCAGGGGCGGCAGCTGCATA+CGG 0.453407 8_2:+5655043 Msa1229720:CDS
AAAGGGCGTGGATCTGGTAT+AGG 0.462773 8_2:+5655010 Msa1229720:intron
CACCATCTGTCGCATGGACA+TGG 0.469597 8_2:+5655070 Msa1229720:CDS
CCACCACATGCTGCAGGAGG+AGG 0.490677 8_2:+5654384 Msa1229720:CDS
TGACCAAATTGAATGCTTGT+TGG 0.494322 8_2:-5653817 None:intergenic
AGATGGTTGATGATGATATC+CGG 0.500001 8_2:-5654433 None:intergenic
TTTGGCAAGCGTTGGAAGCA+TGG 0.515401 8_2:+5655145 Msa1229720:CDS
CATGGTTATGGTTATGGACA+TGG 0.516613 8_2:+5655103 Msa1229720:CDS
CCTCCTTGAGGTGGATATGC+CGG 0.520114 8_2:-5654342 None:intergenic
ACAGACCTAAATAAAGTTGA+GGG 0.520171 8_2:-5655418 None:intergenic
ATGGAAAATTTGGCAAGCGT+TGG 0.520922 8_2:+5655137 Msa1229720:CDS
CCACCTTATCCTCCTCCACC+TGG 0.523771 8_2:+5654276 Msa1229720:CDS
GAATAAGAACCAGGTGGAGG+AGG 0.530977 8_2:-5654285 None:intergenic
CTCCATGTCCATGCGACAGA+TGG 0.531469 8_2:-5655072 None:intergenic
TACTCAACTGAAAATTGCAA+AGG 0.534231 8_2:-5653863 None:intergenic
ATAACCTCCTTGAGGTGGAT+AGG 0.535330 8_2:-5654307 None:intergenic
GCAAGCGTTGGAAGCATGGT+AGG 0.542067 8_2:+5655149 Msa1229720:CDS
AACAGACCTAAATAAAGTTG+AGG 0.548568 8_2:-5655419 None:intergenic
CAATATCCACCACATGCTGC+AGG 0.549438 8_2:+5654378 Msa1229720:CDS
CACCACATGCTGCAGGAGGA+GGG 0.555583 8_2:+5654385 Msa1229720:CDS
TGAGGTGGATATGCCGGTTG+CGG 0.565399 8_2:-5654336 None:intergenic
GGACATGGAGGTTATCATCA+TGG 0.565727 8_2:+5655085 Msa1229720:CDS
GGATATGGATAGCCATATTG+TGG 0.568164 8_2:-5654465 None:intergenic
CAAGCGATGGAAGTGATGCA+GGG 0.575898 8_2:+5655207 Msa1229720:exon
AAGTAGTAATAACTATTGCT+TGG 0.576130 8_2:-5653890 None:intergenic
TACCCTCCTCCTGCAGCATG+TGG 0.578490 8_2:-5654387 None:intergenic
CCTCCTCCTGCAGCATGTGG+TGG 0.578595 8_2:-5654384 None:intergenic
GGACATGGCAAGTATAAGCA+TGG 0.580856 8_2:+5655118 Msa1229720:CDS
TCTTATTCCTATCCACCTCA+AGG 0.583936 8_2:+5654300 Msa1229720:CDS
GTATAGGAGGATTAATTGCA+GGG 0.585893 8_2:+5655026 Msa1229720:CDS
GGGCGTGGATCTGGTATAGG+AGG 0.586096 8_2:+5655013 Msa1229720:intron
TATTGTGGAGCATGATAAGA+TGG 0.586561 8_2:-5654450 None:intergenic
ACATGTCATAAGATATCTCA+TGG 0.598626 8_2:-5655464 None:intergenic
GGTGGATATGCCGGTTGCGG+TGG 0.601680 8_2:-5654333 None:intergenic
GGATAATAACCTCCTTGAGG+TGG 0.602130 8_2:-5654351 None:intergenic
GTGGAAAATTTGGCAAGCGA+TGG 0.603805 8_2:+5655194 Msa1229720:CDS
CCAGGTGGAGGAGGATAAGG+TGG 0.606697 8_2:-5654276 None:intergenic
GGTGCTCACCATCTGTCGCA+TGG 0.609238 8_2:+5655064 Msa1229720:CDS
TAGGAATAAGAACCAGGTGG+AGG 0.612421 8_2:-5654288 None:intergenic
GGATAATAACCTCCTTGAGG+TGG 0.612862 8_2:-5654312 None:intergenic
GATCAAGAATCTAGTGAGAG+AGG 0.615149 8_2:+5654219 Msa1229720:CDS
AGGAGGATTAATTGCAGGGG+CGG 0.617439 8_2:+5655030 Msa1229720:CDS
GAATGAGGATAACCGGAAGG+TGG 0.623841 8_2:-5654411 None:intergenic
GGTGGATAGGAATAAGAACC+AGG 0.628633 8_2:-5654294 None:intergenic
AAGCGATGGAAGTGATGCAG+GGG 0.637098 8_2:+5655208 Msa1229720:exon
TGATGATGATATCCGGAATG+AGG 0.639527 8_2:-5654426 None:intergenic
CAACCGGCATATCCACCTCA+AGG 0.642247 8_2:+5654339 Msa1229720:CDS
CTTTCTATAAAATCATCCAA+AGG 0.660061 8_2:-5653840 None:intergenic
GGGTGAAACAACATGAACAA+TGG 0.660870 8_2:+5654183 Msa1229720:intron
TATCCACCACATGCTGCAGG+AGG 0.666317 8_2:+5654381 Msa1229720:CDS
CCGGAATGAGGATAACCGGA+AGG 0.667322 8_2:-5654414 None:intergenic
GCATGTGGTGGATATTGTGA+AGG 0.678658 8_2:-5654372 None:intergenic
CATCTGTCGCATGGACATGG+AGG 0.679502 8_2:+5655073 Msa1229720:CDS
GAAGGATAATAACCTCCTTG+AGG 0.682876 8_2:-5654354 None:intergenic
TATTCCTATCCACCTCAAGG+AGG 0.698574 8_2:+5654303 Msa1229720:CDS
GGTGGATAATAACCTCCTTG+AGG 0.702028 8_2:-5654315 None:intergenic
GGATAGGAATAAGAACCAGG+TGG 0.722057 8_2:-5654291 None:intergenic
CCGGCATATCCACCTCAAGG+AGG 0.736973 8_2:+5654342 Msa1229720:CDS
TATAGGAGGATTAATTGCAG+GGG 0.742815 8_2:+5655027 Msa1229720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTATTGTTGTTATAAA+GGG + chr8_2:5654993-5655012 Msa1229720:intron 10.0%
!!! TAATTTATTGTTGTTATAAA+GGG + chr8_2:5654993-5655012 Msa1229720:intron 10.0%
!! AATATACTCCATAAAAAATT+GGG - chr8_2:5654510-5654529 None:intergenic 15.0%
!! TAATATACTCCATAAAAAAT+TGG - chr8_2:5654511-5654530 None:intergenic 15.0%
!!! TACTAGTTTCAAATATTATT+AGG + chr8_2:5654557-5654576 Msa1229720:intron 15.0%
!!! ATTTTCTGCTTTTTTTTATT+GGG + chr8_2:5654600-5654619 Msa1229720:intron 15.0%
!!! CTAATTTATTGTTGTTATAA+AGG + chr8_2:5654992-5655011 Msa1229720:intron 15.0%
!! AATATACTCCATAAAAAATT+GGG - chr8_2:5654510-5654529 None:intergenic 15.0%
!! TAATATACTCCATAAAAAAT+TGG - chr8_2:5654511-5654530 None:intergenic 15.0%
!!! TACTAGTTTCAAATATTATT+AGG + chr8_2:5654557-5654576 Msa1229720:intron 15.0%
!!! ATTTTCTGCTTTTTTTTATT+GGG + chr8_2:5654600-5654619 Msa1229720:intron 15.0%
!!! CTAATTTATTGTTGTTATAA+AGG + chr8_2:5654992-5655011 Msa1229720:intron 15.0%
!!! AAAGTTTCTTAGTTTGAATT+TGG + chr8_2:5653960-5653979 Msa1229720:intron 20.0%
!! AGGAAAGAAAAAAAAAACAA+AGG - chr8_2:5654065-5654084 None:intergenic 20.0%
!!! CATTTTCTGCTTTTTTTTAT+TGG + chr8_2:5654599-5654618 Msa1229720:intron 20.0%
!! ATGAACAATTATTGTTCATT+TGG + chr8_2:5655345-5655364 Msa1229720:three_prime_UTR 20.0%
!!! AAAGTTTCTTAGTTTGAATT+TGG + chr8_2:5653960-5653979 Msa1229720:intron 20.0%
!! AGGAAAGAAAAAAAAAACAA+AGG - chr8_2:5654065-5654084 None:intergenic 20.0%
!!! CATTTTCTGCTTTTTTTTAT+TGG + chr8_2:5654599-5654618 Msa1229720:intron 20.0%
!! ATGAACAATTATTGTTCATT+TGG + chr8_2:5655345-5655364 Msa1229720:three_prime_UTR 20.0%
! CTTTCTATAAAATCATCCAA+AGG - chr8_2:5653843-5653862 None:intergenic 25.0%
! AAGTAGTAATAACTATTGCT+TGG - chr8_2:5653893-5653912 None:intergenic 25.0%
! TTTAATTCTTCTTCCTTTCT+TGG + chr8_2:5653993-5654012 Msa1229720:intron 25.0%
! CAAAATCATGCAAAATATCT+TGG - chr8_2:5654025-5654044 None:intergenic 25.0%
!! CAAGATATTTTGCATGATTT+TGG + chr8_2:5654023-5654042 Msa1229720:intron 25.0%
!!! TTGATCATCCCAATTTTTTA+TGG + chr8_2:5654499-5654518 Msa1229720:intron 25.0%
!!! TATAAAGTGTTGTCAAATAG+AGG + chr8_2:5654694-5654713 Msa1229720:intron 25.0%
! AACAACATTGATCACAAAAA+TGG + chr8_2:5654786-5654805 Msa1229720:intron 25.0%
!!! AGATCAATGTTTTTTGCTAA+TGG + chr8_2:5654828-5654847 Msa1229720:intron 25.0%
! TAAAACAAAGACGTAATATC+AGG - chr8_2:5654907-5654926 None:intergenic 25.0%
! AAGTATAAGCATGGAAAATT+TGG + chr8_2:5655127-5655146 Msa1229720:CDS 25.0%
! CTTTCTATAAAATCATCCAA+AGG - chr8_2:5653843-5653862 None:intergenic 25.0%
! AAGTAGTAATAACTATTGCT+TGG - chr8_2:5653893-5653912 None:intergenic 25.0%
! TTTAATTCTTCTTCCTTTCT+TGG + chr8_2:5653993-5654012 Msa1229720:intron 25.0%
! CAAAATCATGCAAAATATCT+TGG - chr8_2:5654025-5654044 None:intergenic 25.0%
!! CAAGATATTTTGCATGATTT+TGG + chr8_2:5654023-5654042 Msa1229720:intron 25.0%
!!! TTGATCATCCCAATTTTTTA+TGG + chr8_2:5654499-5654518 Msa1229720:intron 25.0%
!!! TATAAAGTGTTGTCAAATAG+AGG + chr8_2:5654694-5654713 Msa1229720:intron 25.0%
! AACAACATTGATCACAAAAA+TGG + chr8_2:5654786-5654805 Msa1229720:intron 25.0%
!!! AGATCAATGTTTTTTGCTAA+TGG + chr8_2:5654828-5654847 Msa1229720:intron 25.0%
! TAAAACAAAGACGTAATATC+AGG - chr8_2:5654907-5654926 None:intergenic 25.0%
! AAGTATAAGCATGGAAAATT+TGG + chr8_2:5655127-5655146 Msa1229720:CDS 25.0%
TACTCAACTGAAAATTGCAA+AGG - chr8_2:5653866-5653885 None:intergenic 30.0%
! ATCTTGGAATTTTCCAAGAA+AGG - chr8_2:5654009-5654028 None:intergenic 30.0%
! ATTTTGGATTCCATCAATCA+TGG + chr8_2:5654039-5654058 Msa1229720:intron 30.0%
TGTTTATGTGTTAAGCCAAA+GGG - chr8_2:5654102-5654121 None:intergenic 30.0%
TTGTTTATGTGTTAAGCCAA+AGG - chr8_2:5654103-5654122 None:intergenic 30.0%
! TGCCAATTTCAAACTGTTTT+AGG + chr8_2:5654162-5654181 Msa1229720:intron 30.0%
! GCCAATTTCAAACTGTTTTA+GGG + chr8_2:5654163-5654182 Msa1229720:intron 30.0%
! ACCCTAAAACAGTTTGAAAT+TGG - chr8_2:5654167-5654186 None:intergenic 30.0%
CATCTATTACAGAACAATAG+CGG - chr8_2:5654668-5654687 None:intergenic 30.0%
CAGAATTTGTACAAACTATG+TGG + chr8_2:5654749-5654768 Msa1229720:intron 30.0%
TAACACATGCAAGAAACATT+AGG - chr8_2:5654864-5654883 None:intergenic 30.0%
TATCATCATGGTTATGGTTA+TGG + chr8_2:5655097-5655116 Msa1229720:CDS 30.0%
AAGTTTAAGCGTGGAAAATT+TGG + chr8_2:5655184-5655203 Msa1229720:CDS 30.0%
!!! AGCAAGTTTTGAATTTCAGT+TGG + chr8_2:5655313-5655332 Msa1229720:three_prime_UTR 30.0%
ACAGACCTAAATAAAGTTGA+GGG - chr8_2:5655421-5655440 None:intergenic 30.0%
AACAGACCTAAATAAAGTTG+AGG - chr8_2:5655422-5655441 None:intergenic 30.0%
! CAACTTTATTTAGGTCTGTT+TGG + chr8_2:5655422-5655441 Msa1229720:three_prime_UTR 30.0%
ACATGTCATAAGATATCTCA+TGG - chr8_2:5655467-5655486 None:intergenic 30.0%
TACTCAACTGAAAATTGCAA+AGG - chr8_2:5653866-5653885 None:intergenic 30.0%
! ATCTTGGAATTTTCCAAGAA+AGG - chr8_2:5654009-5654028 None:intergenic 30.0%
! ATTTTGGATTCCATCAATCA+TGG + chr8_2:5654039-5654058 Msa1229720:intron 30.0%
TGTTTATGTGTTAAGCCAAA+GGG - chr8_2:5654102-5654121 None:intergenic 30.0%
TTGTTTATGTGTTAAGCCAA+AGG - chr8_2:5654103-5654122 None:intergenic 30.0%
! TGCCAATTTCAAACTGTTTT+AGG + chr8_2:5654162-5654181 Msa1229720:intron 30.0%
! GCCAATTTCAAACTGTTTTA+GGG + chr8_2:5654163-5654182 Msa1229720:intron 30.0%
! ACCCTAAAACAGTTTGAAAT+TGG - chr8_2:5654167-5654186 None:intergenic 30.0%
CATCTATTACAGAACAATAG+CGG - chr8_2:5654668-5654687 None:intergenic 30.0%
CAGAATTTGTACAAACTATG+TGG + chr8_2:5654749-5654768 Msa1229720:intron 30.0%
TAACACATGCAAGAAACATT+AGG - chr8_2:5654864-5654883 None:intergenic 30.0%
TATCATCATGGTTATGGTTA+TGG + chr8_2:5655097-5655116 Msa1229720:CDS 30.0%
AAGTTTAAGCGTGGAAAATT+TGG + chr8_2:5655184-5655203 Msa1229720:CDS 30.0%
!!! AGCAAGTTTTGAATTTCAGT+TGG + chr8_2:5655313-5655332 Msa1229720:three_prime_UTR 30.0%
ACAGACCTAAATAAAGTTGA+GGG - chr8_2:5655421-5655440 None:intergenic 30.0%
AACAGACCTAAATAAAGTTG+AGG - chr8_2:5655422-5655441 None:intergenic 30.0%
! CAACTTTATTTAGGTCTGTT+TGG + chr8_2:5655422-5655441 Msa1229720:three_prime_UTR 30.0%
ACATGTCATAAGATATCTCA+TGG - chr8_2:5655467-5655486 None:intergenic 30.0%
AAAACAAAGGCCATGATTGA+TGG - chr8_2:5654052-5654071 None:intergenic 35.0%
! AAAGGGTATGGAAGAATCAT+AGG - chr8_2:5654085-5654104 None:intergenic 35.0%
TATGATTCTTCCATACCCTT+TGG + chr8_2:5654084-5654103 Msa1229720:intron 35.0%
! GGTCTGTTTTCACATATAAC+AGG + chr8_2:5654240-5654259 Msa1229720:CDS 35.0%
!! AGATGGTTGATGATGATATC+CGG - chr8_2:5654436-5654455 None:intergenic 35.0%
TATTGTGGAGCATGATAAGA+TGG - chr8_2:5654453-5654472 None:intergenic 35.0%
TCTTATCATGCTCCACAATA+TGG + chr8_2:5654453-5654472 Msa1229720:CDS 35.0%
CAAAGACGTAATATCAGGTA+TGG - chr8_2:5654902-5654921 None:intergenic 35.0%
! TATTGTTGTTATAAAGGGCG+TGG + chr8_2:5654998-5655017 Msa1229720:intron 35.0%
GTATAGGAGGATTAATTGCA+GGG + chr8_2:5655026-5655045 Msa1229720:CDS 35.0%
TATAGGAGGATTAATTGCAG+GGG + chr8_2:5655027-5655046 Msa1229720:CDS 35.0%
! GTCTTTTGTTCAAATGTGTG+TGG + chr8_2:5655375-5655394 Msa1229720:three_prime_UTR 35.0%
! GAAAGCCCTCAACTTTATTT+AGG + chr8_2:5655413-5655432 Msa1229720:three_prime_UTR 35.0%
TGACCAAATTGAATGCTTGT+TGG - chr8_2:5653820-5653839 None:intergenic 35.0%
AAAACAAAGGCCATGATTGA+TGG - chr8_2:5654052-5654071 None:intergenic 35.0%
! AAAGGGTATGGAAGAATCAT+AGG - chr8_2:5654085-5654104 None:intergenic 35.0%
TATGATTCTTCCATACCCTT+TGG + chr8_2:5654084-5654103 Msa1229720:intron 35.0%
! GGTCTGTTTTCACATATAAC+AGG + chr8_2:5654240-5654259 Msa1229720:CDS 35.0%
!! AGATGGTTGATGATGATATC+CGG - chr8_2:5654436-5654455 None:intergenic 35.0%
TATTGTGGAGCATGATAAGA+TGG - chr8_2:5654453-5654472 None:intergenic 35.0%
TCTTATCATGCTCCACAATA+TGG + chr8_2:5654453-5654472 Msa1229720:CDS 35.0%
CAAAGACGTAATATCAGGTA+TGG - chr8_2:5654902-5654921 None:intergenic 35.0%
! TATTGTTGTTATAAAGGGCG+TGG + chr8_2:5654998-5655017 Msa1229720:intron 35.0%
GTATAGGAGGATTAATTGCA+GGG + chr8_2:5655026-5655045 Msa1229720:CDS 35.0%
TATAGGAGGATTAATTGCAG+GGG + chr8_2:5655027-5655046 Msa1229720:CDS 35.0%
! GTCTTTTGTTCAAATGTGTG+TGG + chr8_2:5655375-5655394 Msa1229720:three_prime_UTR 35.0%
! GAAAGCCCTCAACTTTATTT+AGG + chr8_2:5655413-5655432 Msa1229720:three_prime_UTR 35.0%
GCATTCAATTTGGTCACCTT+TGG + chr8_2:5653824-5653843 Msa1229720:five_prime_UTR 40.0%
ATGTGTTAAGCCAAAGGGTA+TGG - chr8_2:5654097-5654116 None:intergenic 40.0%
GGGTGAAACAACATGAACAA+TGG + chr8_2:5654183-5654202 Msa1229720:intron 40.0%
GATCAAGAATCTAGTGAGAG+AGG + chr8_2:5654219-5654238 Msa1229720:CDS 40.0%
TCTTATTCCTATCCACCTCA+AGG + chr8_2:5654300-5654319 Msa1229720:CDS 40.0%
GAAGGATAATAACCTCCTTG+AGG - chr8_2:5654357-5654376 None:intergenic 40.0%
ATATCCGGAATGAGGATAAC+CGG - chr8_2:5654421-5654440 None:intergenic 40.0%
! TGATGATGATATCCGGAATG+AGG - chr8_2:5654429-5654448 None:intergenic 40.0%
GGATATGGATAGCCATATTG+TGG - chr8_2:5654468-5654487 None:intergenic 40.0%
TATGGCTATCCATATCCTTC+TGG + chr8_2:5654471-5654490 Msa1229720:CDS 40.0%
TCAAGTGTACCAGAAGGATA+TGG - chr8_2:5654483-5654502 None:intergenic 40.0%
!! CGGCTTGTTAAAATTCTGCT+AGG - chr8_2:5654648-5654667 None:intergenic 40.0%
GGTATAGGAGGATTAATTGC+AGG + chr8_2:5655025-5655044 Msa1229720:CDS 40.0%
GGAGGTTATCATCATGGTTA+TGG + chr8_2:5655091-5655110 Msa1229720:CDS 40.0%
CATGGTTATGGTTATGGACA+TGG + chr8_2:5655103-5655122 Msa1229720:CDS 40.0%
ATGGAAAATTTGGCAAGCGT+TGG + chr8_2:5655137-5655156 Msa1229720:CDS 40.0%
!! AAGCATGGTAGGTTTGGTTT+TGG + chr8_2:5655160-5655179 Msa1229720:CDS 40.0%
!!! GGTTTTGGAAAGTTTAAGCG+TGG + chr8_2:5655175-5655194 Msa1229720:CDS 40.0%
GCATTCAATTTGGTCACCTT+TGG + chr8_2:5653824-5653843 Msa1229720:five_prime_UTR 40.0%
ATGTGTTAAGCCAAAGGGTA+TGG - chr8_2:5654097-5654116 None:intergenic 40.0%
GGGTGAAACAACATGAACAA+TGG + chr8_2:5654183-5654202 Msa1229720:intron 40.0%
GATCAAGAATCTAGTGAGAG+AGG + chr8_2:5654219-5654238 Msa1229720:CDS 40.0%
TCTTATTCCTATCCACCTCA+AGG + chr8_2:5654300-5654319 Msa1229720:CDS 40.0%
GAAGGATAATAACCTCCTTG+AGG - chr8_2:5654357-5654376 None:intergenic 40.0%
ATATCCGGAATGAGGATAAC+CGG - chr8_2:5654421-5654440 None:intergenic 40.0%
! TGATGATGATATCCGGAATG+AGG - chr8_2:5654429-5654448 None:intergenic 40.0%
GGATATGGATAGCCATATTG+TGG - chr8_2:5654468-5654487 None:intergenic 40.0%
TATGGCTATCCATATCCTTC+TGG + chr8_2:5654471-5654490 Msa1229720:CDS 40.0%
TCAAGTGTACCAGAAGGATA+TGG - chr8_2:5654483-5654502 None:intergenic 40.0%
!! CGGCTTGTTAAAATTCTGCT+AGG - chr8_2:5654648-5654667 None:intergenic 40.0%
GGTATAGGAGGATTAATTGC+AGG + chr8_2:5655025-5655044 Msa1229720:CDS 40.0%
GGAGGTTATCATCATGGTTA+TGG + chr8_2:5655091-5655110 Msa1229720:CDS 40.0%
CATGGTTATGGTTATGGACA+TGG + chr8_2:5655103-5655122 Msa1229720:CDS 40.0%
ATGGAAAATTTGGCAAGCGT+TGG + chr8_2:5655137-5655156 Msa1229720:CDS 40.0%
!! AAGCATGGTAGGTTTGGTTT+TGG + chr8_2:5655160-5655179 Msa1229720:CDS 40.0%
!!! GGTTTTGGAAAGTTTAAGCG+TGG + chr8_2:5655175-5655194 Msa1229720:CDS 40.0%
TAGGAATAAGAACCAGGTGG+AGG - chr8_2:5654291-5654310 None:intergenic 45.0%
GGATAGGAATAAGAACCAGG+TGG - chr8_2:5654294-5654313 None:intergenic 45.0%
GGTGGATAGGAATAAGAACC+AGG - chr8_2:5654297-5654316 None:intergenic 45.0%
TATTCCTATCCACCTCAAGG+AGG + chr8_2:5654303-5654322 Msa1229720:CDS 45.0%
ATAACCTCCTTGAGGTGGAT+AGG - chr8_2:5654310-5654329 None:intergenic 45.0%
GGATAATAACCTCCTTGAGG+TGG - chr8_2:5654315-5654334 None:intergenic 45.0%
GGTGGATAATAACCTCCTTG+AGG - chr8_2:5654318-5654337 None:intergenic 45.0%
AGGTTATTATCCACCGCAAC+CGG + chr8_2:5654323-5654342 Msa1229720:CDS 45.0%
GGATAATAACCTCCTTGAGG+TGG - chr8_2:5654315-5654334 None:intergenic 45.0%
!! GCATGTGGTGGATATTGTGA+AGG - chr8_2:5654375-5654394 None:intergenic 45.0%
GGATGATCAAGTGTACCAGA+AGG - chr8_2:5654489-5654508 None:intergenic 45.0%
CAGAATGATGCTCGTTTGAG+AGG + chr8_2:5654950-5654969 Msa1229720:intron 45.0%
! TGTTATAAAGGGCGTGGATC+TGG + chr8_2:5655004-5655023 Msa1229720:intron 45.0%
GGACATGGAGGTTATCATCA+TGG + chr8_2:5655085-5655104 Msa1229720:CDS 45.0%
GGACATGGCAAGTATAAGCA+TGG + chr8_2:5655118-5655137 Msa1229720:CDS 45.0%
GTGGAAAATTTGGCAAGCGA+TGG + chr8_2:5655194-5655213 Msa1229720:CDS 45.0%
TAGGAATAAGAACCAGGTGG+AGG - chr8_2:5654291-5654310 None:intergenic 45.0%
GGATAGGAATAAGAACCAGG+TGG - chr8_2:5654294-5654313 None:intergenic 45.0%
GGTGGATAGGAATAAGAACC+AGG - chr8_2:5654297-5654316 None:intergenic 45.0%
TATTCCTATCCACCTCAAGG+AGG + chr8_2:5654303-5654322 Msa1229720:CDS 45.0%
ATAACCTCCTTGAGGTGGAT+AGG - chr8_2:5654310-5654329 None:intergenic 45.0%
GGATAATAACCTCCTTGAGG+TGG - chr8_2:5654315-5654334 None:intergenic 45.0%
GGTGGATAATAACCTCCTTG+AGG - chr8_2:5654318-5654337 None:intergenic 45.0%
AGGTTATTATCCACCGCAAC+CGG + chr8_2:5654323-5654342 Msa1229720:CDS 45.0%
GGATAATAACCTCCTTGAGG+TGG - chr8_2:5654315-5654334 None:intergenic 45.0%
!! GCATGTGGTGGATATTGTGA+AGG - chr8_2:5654375-5654394 None:intergenic 45.0%
GGATGATCAAGTGTACCAGA+AGG - chr8_2:5654489-5654508 None:intergenic 45.0%
CAGAATGATGCTCGTTTGAG+AGG + chr8_2:5654950-5654969 Msa1229720:intron 45.0%
! TGTTATAAAGGGCGTGGATC+TGG + chr8_2:5655004-5655023 Msa1229720:intron 45.0%
GGACATGGAGGTTATCATCA+TGG + chr8_2:5655085-5655104 Msa1229720:CDS 45.0%
GGACATGGCAAGTATAAGCA+TGG + chr8_2:5655118-5655137 Msa1229720:CDS 45.0%
GTGGAAAATTTGGCAAGCGA+TGG + chr8_2:5655194-5655213 Msa1229720:CDS 45.0%
GAATAAGAACCAGGTGGAGG+AGG - chr8_2:5654288-5654307 None:intergenic 50.0%
CAATATCCACCACATGCTGC+AGG + chr8_2:5654378-5654397 Msa1229720:CDS 50.0%
GAATGAGGATAACCGGAAGG+TGG - chr8_2:5654414-5654433 None:intergenic 50.0%
CCTTCCGGTTATCCTCATTC+CGG + chr8_2:5654414-5654433 Msa1229720:CDS 50.0%
! AAAGGGCGTGGATCTGGTAT+AGG + chr8_2:5655010-5655029 Msa1229720:intron 50.0%
AGGAGGATTAATTGCAGGGG+CGG + chr8_2:5655030-5655049 Msa1229720:CDS 50.0%
!! TTTGGCAAGCGTTGGAAGCA+TGG + chr8_2:5655145-5655164 Msa1229720:CDS 50.0%
! CGTTGGAAGCATGGTAGGTT+TGG + chr8_2:5655154-5655173 Msa1229720:CDS 50.0%
CAAGCGATGGAAGTGATGCA+GGG + chr8_2:5655207-5655226 Msa1229720:exon 50.0%
AAGCGATGGAAGTGATGCAG+GGG + chr8_2:5655208-5655227 Msa1229720:exon 50.0%
GAATAAGAACCAGGTGGAGG+AGG - chr8_2:5654288-5654307 None:intergenic 50.0%
CAATATCCACCACATGCTGC+AGG + chr8_2:5654378-5654397 Msa1229720:CDS 50.0%
GAATGAGGATAACCGGAAGG+TGG - chr8_2:5654414-5654433 None:intergenic 50.0%
CCTTCCGGTTATCCTCATTC+CGG + chr8_2:5654414-5654433 Msa1229720:CDS 50.0%
! AAAGGGCGTGGATCTGGTAT+AGG + chr8_2:5655010-5655029 Msa1229720:intron 50.0%
AGGAGGATTAATTGCAGGGG+CGG + chr8_2:5655030-5655049 Msa1229720:CDS 50.0%
!! TTTGGCAAGCGTTGGAAGCA+TGG + chr8_2:5655145-5655164 Msa1229720:CDS 50.0%
! CGTTGGAAGCATGGTAGGTT+TGG + chr8_2:5655154-5655173 Msa1229720:CDS 50.0%
CAAGCGATGGAAGTGATGCA+GGG + chr8_2:5655207-5655226 Msa1229720:exon 50.0%
AAGCGATGGAAGTGATGCAG+GGG + chr8_2:5655208-5655227 Msa1229720:exon 50.0%
GAACCAGGTGGAGGAGGATA+AGG - chr8_2:5654282-5654301 None:intergenic 55.0%
! TGAGGTGGATATGCCGGTTG+CGG - chr8_2:5654339-5654358 None:intergenic 55.0%
CAACCGGCATATCCACCTCA+AGG + chr8_2:5654339-5654358 Msa1229720:CDS 55.0%
CCTCCTTGAGGTGGATATGC+CGG - chr8_2:5654345-5654364 None:intergenic 55.0%
TATCCACCACATGCTGCAGG+AGG + chr8_2:5654381-5654400 Msa1229720:CDS 55.0%
CCGGAATGAGGATAACCGGA+AGG - chr8_2:5654417-5654436 None:intergenic 55.0%
CACCATCTGTCGCATGGACA+TGG + chr8_2:5655070-5655089 Msa1229720:CDS 55.0%
CTCCATGTCCATGCGACAGA+TGG - chr8_2:5655075-5655094 None:intergenic 55.0%
CATCTGTCGCATGGACATGG+AGG + chr8_2:5655073-5655092 Msa1229720:CDS 55.0%
!! GCAAGCGTTGGAAGCATGGT+AGG + chr8_2:5655149-5655168 Msa1229720:CDS 55.0%
GCAAGCGATGGAAGTGATGC+AGG + chr8_2:5655206-5655225 Msa1229720:exon 55.0%
GAACCAGGTGGAGGAGGATA+AGG - chr8_2:5654282-5654301 None:intergenic 55.0%
! TGAGGTGGATATGCCGGTTG+CGG - chr8_2:5654339-5654358 None:intergenic 55.0%
CAACCGGCATATCCACCTCA+AGG + chr8_2:5654339-5654358 Msa1229720:CDS 55.0%
CCTCCTTGAGGTGGATATGC+CGG - chr8_2:5654345-5654364 None:intergenic 55.0%
TATCCACCACATGCTGCAGG+AGG + chr8_2:5654381-5654400 Msa1229720:CDS 55.0%
CCGGAATGAGGATAACCGGA+AGG - chr8_2:5654417-5654436 None:intergenic 55.0%
CACCATCTGTCGCATGGACA+TGG + chr8_2:5655070-5655089 Msa1229720:CDS 55.0%
CTCCATGTCCATGCGACAGA+TGG - chr8_2:5655075-5655094 None:intergenic 55.0%
CATCTGTCGCATGGACATGG+AGG + chr8_2:5655073-5655092 Msa1229720:CDS 55.0%
!! GCAAGCGTTGGAAGCATGGT+AGG + chr8_2:5655149-5655168 Msa1229720:CDS 55.0%
GCAAGCGATGGAAGTGATGC+AGG + chr8_2:5655206-5655225 Msa1229720:exon 55.0%
!! GGAGGATAAGGTGGTGCTGC+TGG - chr8_2:5654270-5654289 None:intergenic 60.0%
CCAGGTGGAGGAGGATAAGG+TGG - chr8_2:5654279-5654298 None:intergenic 60.0%
CCACCTTATCCTCCTCCACC+TGG + chr8_2:5654276-5654295 Msa1229720:CDS 60.0%
CCGGCATATCCACCTCAAGG+AGG + chr8_2:5654342-5654361 Msa1229720:CDS 60.0%
CACCACATGCTGCAGGAGGA+GGG + chr8_2:5654385-5654404 Msa1229720:CDS 60.0%
TACCCTCCTCCTGCAGCATG+TGG - chr8_2:5654390-5654409 None:intergenic 60.0%
!! GGAGGAGGGTATCCACCTTC+CGG + chr8_2:5654399-5654418 Msa1229720:CDS 60.0%
GGGCGTGGATCTGGTATAGG+AGG + chr8_2:5655013-5655032 Msa1229720:intron 60.0%
GGTGCTCACCATCTGTCGCA+TGG + chr8_2:5655064-5655083 Msa1229720:CDS 60.0%
!! GGAGGATAAGGTGGTGCTGC+TGG - chr8_2:5654270-5654289 None:intergenic 60.0%
CCAGGTGGAGGAGGATAAGG+TGG - chr8_2:5654279-5654298 None:intergenic 60.0%
CCACCTTATCCTCCTCCACC+TGG + chr8_2:5654276-5654295 Msa1229720:CDS 60.0%
CCGGCATATCCACCTCAAGG+AGG + chr8_2:5654342-5654361 Msa1229720:CDS 60.0%
CACCACATGCTGCAGGAGGA+GGG + chr8_2:5654385-5654404 Msa1229720:CDS 60.0%
TACCCTCCTCCTGCAGCATG+TGG - chr8_2:5654390-5654409 None:intergenic 60.0%
!! GGAGGAGGGTATCCACCTTC+CGG + chr8_2:5654399-5654418 Msa1229720:CDS 60.0%
GGGCGTGGATCTGGTATAGG+AGG + chr8_2:5655013-5655032 Msa1229720:intron 60.0%
GGTGCTCACCATCTGTCGCA+TGG + chr8_2:5655064-5655083 Msa1229720:CDS 60.0%
! GGTGGATATGCCGGTTGCGG+TGG - chr8_2:5654336-5654355 None:intergenic 65.0%
CCTCCTCCTGCAGCATGTGG+TGG - chr8_2:5654387-5654406 None:intergenic 65.0%
CCACCACATGCTGCAGGAGG+AGG + chr8_2:5654384-5654403 Msa1229720:CDS 65.0%
! GGTGGATATGCCGGTTGCGG+TGG - chr8_2:5654336-5654355 None:intergenic 65.0%
CCTCCTCCTGCAGCATGTGG+TGG - chr8_2:5654387-5654406 None:intergenic 65.0%
CCACCACATGCTGCAGGAGG+AGG + chr8_2:5654384-5654403 Msa1229720:CDS 65.0%
GCAGGGGCGGCAGCTGCATA+CGG + chr8_2:5655043-5655062 Msa1229720:CDS 70.0%
GCAGGGGCGGCAGCTGCATA+CGG + chr8_2:5655043-5655062 Msa1229720:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 5653818 5655501 5653818 ID=Msa1229720;Name=Msa1229720
chr8_2 mRNA 5653818 5655501 5653818 ID=Msa1229720-mRNA-1;Parent=Msa1229720;Name=Msa1229720-mRNA-1;_AED=0.04;_eAED=0.04;_QI=93|1|1|1|0.5|0.33|3|279|168
chr8_2 exon 5653818 5653899 5653818 ID=Msa1229720-mRNA-1:exon:1922;Parent=Msa1229720-mRNA-1
chr8_2 exon 5654184 5654492 5654184 ID=Msa1229720-mRNA-1:exon:1923;Parent=Msa1229720-mRNA-1
chr8_2 exon 5655014 5655501 5655014 ID=Msa1229720-mRNA-1:exon:1924;Parent=Msa1229720-mRNA-1
chr8_2 five_prime_UTR 5653818 5653899 5653818 ID=Msa1229720-mRNA-1:five_prime_utr;Parent=Msa1229720-mRNA-1
chr8_2 five_prime_UTR 5654184 5654194 5654184 ID=Msa1229720-mRNA-1:five_prime_utr;Parent=Msa1229720-mRNA-1
chr8_2 CDS 5654195 5654492 5654195 ID=Msa1229720-mRNA-1:cds;Parent=Msa1229720-mRNA-1
chr8_2 CDS 5655014 5655222 5655014 ID=Msa1229720-mRNA-1:cds;Parent=Msa1229720-mRNA-1
chr8_2 three_prime_UTR 5655223 5655501 5655223 ID=Msa1229720-mRNA-1:three_prime_utr;Parent=Msa1229720-mRNA-1
Gene Sequence

>Msa1229720

ATGAACAATGGTAGAAACAAGCATGATCAAGAATCTAGTGAGAGAGGTCTGTTTTCACATATAACAGGCTATCCAGCAGCACCACCTTATCCTCCTCCACCTGGTTCTTATTCCTATCCACCTCAAGGAGGTTATTATCCACCGCAACCGGCATATCCACCTCAAGGAGGTTATTATCCTTCACAATATCCACCACATGCTGCAGGAGGAGGGTATCCACCTTCCGGTTATCCTCATTCCGGATATCATCATCAACCATCTTATCATGCTCCACAATATGGCTATCCATATCCTTCTGGGCGTGGATCTGGTATAGGAGGATTAATTGCAGGGGCGGCAGCTGCATACGGTGCTCACCATCTGTCGCATGGACATGGAGGTTATCATCATGGTTATGGTTATGGACATGGCAAGTATAAGCATGGAAAATTTGGCAAGCGTTGGAAGCATGGTAGGTTTGGTTTTGGAAAGTTTAAGCGTGGAAAATTTGGCAAGCGATGGAAGTGA
Protein sequence

>Msa1229720

MNNGRNKHDQESSERGLFSHITGYPAAPPYPPPPGSYSYPPQGGYYPPQPAYPPQGGYYPSQYPPHAAGGGYPPSGYPHSGYHHQPSYHAPQYGYPYPSGRGSGIGGLIAGAAAAYGAHHLSHGHGGYHHGYGYGHGKYKHGKFGKRWKHGRFGFGKFKRGKFGKRWK*