AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0689370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0689370 MtrunA17_Chr8g0337261 85.882 170 4 3 1 168 1 152 1.61e-55 172
Msa0689370 MtrunA17_Chr8g0337251 64.286 168 23 8 1 168 1 131 1.11e-31 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 77 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCCCTCAACTTTATTT+AGG 0.238868 4_4:-56426218 Msa0689370:three_prime_UTR
AAGTTTAAGCGTGGAAAATT+TGG 0.266461 4_4:-56426447 Msa0689370:CDS
CAACTTTATTTAGGTCTGTT+TGG 0.271608 4_4:-56426209 Msa0689370:three_prime_UTR
ATGAACAATTATTGTTCATT+TGG 0.293223 4_4:-56426286 Msa0689370:three_prime_UTR
AAGTATAAGCATGGAAAATT+TGG 0.307873 4_4:-56426504 Msa0689370:CDS
TATCATCATGGTTATGGTTA+TGG 0.313342 4_4:-56426534 Msa0689370:CDS
AAGCATGGTAGGTTTGGTTT+TGG 0.332336 4_4:-56426471 Msa0689370:CDS
TCTTATCATGCTCCACAATA+TGG 0.348923 4_4:-56427178 Msa0689370:CDS
TATGGCTATCCATATCCTTC+TGG 0.365272 4_4:-56427160 Msa0689370:intron
GGAGGTTATCATCATGGTTA+TGG 0.376411 4_4:-56426540 Msa0689370:CDS
CGTTGGAAGCATGGTAGGTT+TGG 0.389621 4_4:-56426477 Msa0689370:CDS
TCAAGTGTACCAGAAGGATA+TGG 0.413672 4_4:+56427151 None:intergenic
CCTTCCGGTTATCCTCATTC+CGG 0.421172 4_4:-56427217 Msa0689370:CDS
GAACCAGGTGGAGGAGGATA+AGG 0.429948 4_4:+56427352 None:intergenic
GGAGGATAAGGTGGTGCTGC+TGG 0.432218 4_4:+56427364 None:intergenic
TGTTATAAAGGGCGTGGATC+TGG 0.441402 4_4:-56426627 Msa0689370:intron
ATATCCGGAATGAGGATAAC+CGG 0.442176 4_4:+56427213 None:intergenic
GGTATAGGAGGATTAATTGC+AGG 0.442489 4_4:-56426606 Msa0689370:CDS
GCAAGCGATGGAAGTGATGC+AGG 0.445817 4_4:-56426425 Msa0689370:exon
AGGTTATTATCCACCGCAAC+CGG 0.446409 4_4:-56427308 Msa0689370:CDS
GGAGGAGGGTATCCACCTTC+CGG 0.452585 4_4:-56427232 Msa0689370:CDS
GCAGGGGCGGCAGCTGCATA+CGG 0.453407 4_4:-56426588 Msa0689370:CDS
AAAGGGCGTGGATCTGGTAT+AGG 0.462773 4_4:-56426621 Msa0689370:intron
CACCATCTGTCGCATGGACA+TGG 0.469597 4_4:-56426561 Msa0689370:CDS
CATGGTTATGGTTATGGACA+TGG 0.469853 4_4:-56426528 Msa0689370:CDS
CCACCACATGCTGCAGGAGG+AGG 0.490677 4_4:-56427247 Msa0689370:CDS
AGATGGTTGATGATGATATC+CGG 0.500001 4_4:+56427198 None:intergenic
TTTGGCAAGCGTTGGAAGCA+TGG 0.515401 4_4:-56426486 Msa0689370:CDS
CCTCCTTGAGGTGGATATGC+CGG 0.520114 4_4:+56427289 None:intergenic
ACAGACCTAAATAAAGTTGA+GGG 0.520171 4_4:+56426213 None:intergenic
ATGGAAAATTTGGCAAGCGT+TGG 0.520922 4_4:-56426494 Msa0689370:CDS
CCACCTTATCCTCCTCCACC+TGG 0.523771 4_4:-56427355 Msa0689370:CDS
GAATAAGAACCAGGTGGAGG+AGG 0.530977 4_4:+56427346 None:intergenic
CTCCATGTCCATGCGACAGA+TGG 0.531469 4_4:+56426559 None:intergenic
ATAACCTCCTTGAGGTGGAT+AGG 0.535330 4_4:+56427324 None:intergenic
GCAAGCGTTGGAAGCATGGT+AGG 0.542067 4_4:-56426482 Msa0689370:CDS
AACAGACCTAAATAAAGTTG+AGG 0.548568 4_4:+56426212 None:intergenic
CAATATCCACCACATGCTGC+AGG 0.549438 4_4:-56427253 Msa0689370:CDS
CACCACATGCTGCAGGAGGA+GGG 0.555583 4_4:-56427246 Msa0689370:CDS
TGAGGTGGATATGCCGGTTG+CGG 0.565399 4_4:+56427295 None:intergenic
GGACATGGAGGTTATCATCA+TGG 0.565727 4_4:-56426546 Msa0689370:CDS
GGATATGGATAGCCATATTG+TGG 0.568164 4_4:+56427166 None:intergenic
CAAGCGATGGAAGTGATGCA+GGG 0.575898 4_4:-56426424 Msa0689370:exon
TACCCTCCTCCTGCAGCATG+TGG 0.578490 4_4:+56427244 None:intergenic
CCTCCTCCTGCAGCATGTGG+TGG 0.578595 4_4:+56427247 None:intergenic
TCTTATTCCTATCCACCTCA+AGG 0.583936 4_4:-56427331 Msa0689370:CDS
GTATAGGAGGATTAATTGCA+GGG 0.585893 4_4:-56426605 Msa0689370:CDS
GGGCGTGGATCTGGTATAGG+AGG 0.586096 4_4:-56426618 Msa0689370:CDS
TATTGTGGAGCATGATAAGA+TGG 0.586561 4_4:+56427181 None:intergenic
ACATGTCATAAGATATCTCA+TGG 0.598626 4_4:+56426167 None:intergenic
GGTGGATATGCCGGTTGCGG+TGG 0.601680 4_4:+56427298 None:intergenic
GGATAATAACCTCCTTGAGG+TGG 0.602130 4_4:+56427280 None:intergenic
GTGGAAAATTTGGCAAGCGA+TGG 0.603805 4_4:-56426437 Msa0689370:CDS
CCAGGTGGAGGAGGATAAGG+TGG 0.606697 4_4:+56427355 None:intergenic
GGACATGGGAAGTATAAGCA+TGG 0.607097 4_4:-56426513 Msa0689370:CDS
GGTGCTCACCATCTGTCGCA+TGG 0.609238 4_4:-56426567 Msa0689370:CDS
TAGGAATAAGAACCAGGTGG+AGG 0.612421 4_4:+56427343 None:intergenic
GGATAATAACCTCCTTGAGG+TGG 0.612862 4_4:+56427319 None:intergenic
GATCAAGAATCTAGTGAGAG+AGG 0.615149 4_4:-56427412 Msa0689370:CDS
AGGAGGATTAATTGCAGGGG+CGG 0.617439 4_4:-56426601 Msa0689370:CDS
ATGGTTATGGTTATGGACAT+GGG 0.618834 4_4:-56426527 Msa0689370:CDS
GAATGAGGATAACCGGAAGG+TGG 0.623841 4_4:+56427220 None:intergenic
GGTGGATAGGAATAAGAACC+AGG 0.628633 4_4:+56427337 None:intergenic
AAGCGATGGAAGTGATGCAG+GGG 0.637098 4_4:-56426423 Msa0689370:exon
TGATGATGATATCCGGAATG+AGG 0.639527 4_4:+56427205 None:intergenic
CAACCGGCATATCCACCTCA+AGG 0.642247 4_4:-56427292 Msa0689370:CDS
GGGTGAAACAACATGAACAA+TGG 0.660870 4_4:-56427448 None:intergenic
TATCCACCACATGCTGCAGG+AGG 0.666317 4_4:-56427250 Msa0689370:CDS
CCGGAATGAGGATAACCGGA+AGG 0.667322 4_4:+56427217 None:intergenic
GCATGTGGTGGATATTGTGA+AGG 0.678658 4_4:+56427259 None:intergenic
CATCTGTCGCATGGACATGG+AGG 0.679502 4_4:-56426558 Msa0689370:CDS
GAAGGATAATAACCTCCTTG+AGG 0.682876 4_4:+56427277 None:intergenic
TATTCCTATCCACCTCAAGG+AGG 0.698574 4_4:-56427328 Msa0689370:CDS
GGTGGATAATAACCTCCTTG+AGG 0.702028 4_4:+56427316 None:intergenic
GGATAGGAATAAGAACCAGG+TGG 0.722057 4_4:+56427340 None:intergenic
CCGGCATATCCACCTCAAGG+AGG 0.736973 4_4:-56427289 Msa0689370:CDS
TATAGGAGGATTAATTGCAG+GGG 0.742815 4_4:-56426604 Msa0689370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTATTGTTGTTATAAA+GGG - chr4_4:56426974-56426993 Msa0689370:intron 10.0%
!!! TAATTTATTGTTGTTATAAA+GGG - chr4_4:56426974-56426993 Msa0689370:intron 10.0%
!! AATATACTCCATAAAAAATT+GGG + chr4_4:56426491-56426510 None:intergenic 15.0%
!! TAATATACTCCATAAAAAAT+TGG + chr4_4:56426492-56426511 None:intergenic 15.0%
!!! TACTAGTTTCAAATATTATT+AGG - chr4_4:56426538-56426557 Msa0689370:CDS 15.0%
!!! ATTTTCTGCTTTTTTTTATT+GGG - chr4_4:56426581-56426600 Msa0689370:CDS 15.0%
!!! CTAATTTATTGTTGTTATAA+AGG - chr4_4:56426973-56426992 Msa0689370:intron 15.0%
!! AATATACTCCATAAAAAATT+GGG + chr4_4:56426491-56426510 None:intergenic 15.0%
!! TAATATACTCCATAAAAAAT+TGG + chr4_4:56426492-56426511 None:intergenic 15.0%
!!! TACTAGTTTCAAATATTATT+AGG - chr4_4:56426538-56426557 Msa0689370:CDS 15.0%
!!! ATTTTCTGCTTTTTTTTATT+GGG - chr4_4:56426581-56426600 Msa0689370:CDS 15.0%
!!! CTAATTTATTGTTGTTATAA+AGG - chr4_4:56426973-56426992 Msa0689370:intron 15.0%
!!! CATTTTCTGCTTTTTTTTAT+TGG - chr4_4:56426580-56426599 Msa0689370:CDS 20.0%
!! ATGAACAATTATTGTTCATT+TGG - chr4_4:56427326-56427345 Msa0689370:CDS 20.0%
!!! CATTTTCTGCTTTTTTTTAT+TGG - chr4_4:56426580-56426599 Msa0689370:CDS 20.0%
!! ATGAACAATTATTGTTCATT+TGG - chr4_4:56427326-56427345 Msa0689370:CDS 20.0%
!!! TTGATCATCCCAATTTTTTA+TGG - chr4_4:56426480-56426499 Msa0689370:CDS 25.0%
!!! TATAAAGTGTTGTCAAATAG+AGG - chr4_4:56426675-56426694 Msa0689370:intron 25.0%
! AACAACATTGATCACAAAAA+TGG - chr4_4:56426767-56426786 Msa0689370:intron 25.0%
!!! AGATCAATGTTTTTTGCTAA+TGG - chr4_4:56426809-56426828 Msa0689370:intron 25.0%
! TAAAACAAAGACGTAATATC+AGG + chr4_4:56426888-56426907 None:intergenic 25.0%
! AAGTATAAGCATGGAAAATT+TGG - chr4_4:56427108-56427127 Msa0689370:intron 25.0%
!!! TTGATCATCCCAATTTTTTA+TGG - chr4_4:56426480-56426499 Msa0689370:CDS 25.0%
!!! TATAAAGTGTTGTCAAATAG+AGG - chr4_4:56426675-56426694 Msa0689370:intron 25.0%
! AACAACATTGATCACAAAAA+TGG - chr4_4:56426767-56426786 Msa0689370:intron 25.0%
!!! AGATCAATGTTTTTTGCTAA+TGG - chr4_4:56426809-56426828 Msa0689370:intron 25.0%
! TAAAACAAAGACGTAATATC+AGG + chr4_4:56426888-56426907 None:intergenic 25.0%
! AAGTATAAGCATGGAAAATT+TGG - chr4_4:56427108-56427127 Msa0689370:intron 25.0%
CATCTATTACAGAACAATAG+CGG + chr4_4:56426649-56426668 None:intergenic 30.0%
CAGAATTTGTACAAACTATG+TGG - chr4_4:56426730-56426749 Msa0689370:intron 30.0%
TAACACATGCAAGAAACATT+AGG + chr4_4:56426845-56426864 None:intergenic 30.0%
TATCATCATGGTTATGGTTA+TGG - chr4_4:56427078-56427097 Msa0689370:intron 30.0%
AAGTTTAAGCGTGGAAAATT+TGG - chr4_4:56427165-56427184 Msa0689370:CDS 30.0%
!!! AGCAAGTTTTGAATTTCAGT+TGG - chr4_4:56427294-56427313 Msa0689370:CDS 30.0%
ACAGACCTAAATAAAGTTGA+GGG + chr4_4:56427402-56427421 None:intergenic 30.0%
AACAGACCTAAATAAAGTTG+AGG + chr4_4:56427403-56427422 None:intergenic 30.0%
! CAACTTTATTTAGGTCTGTT+TGG - chr4_4:56427403-56427422 Msa0689370:CDS 30.0%
CATCTATTACAGAACAATAG+CGG + chr4_4:56426649-56426668 None:intergenic 30.0%
CAGAATTTGTACAAACTATG+TGG - chr4_4:56426730-56426749 Msa0689370:intron 30.0%
TAACACATGCAAGAAACATT+AGG + chr4_4:56426845-56426864 None:intergenic 30.0%
TATCATCATGGTTATGGTTA+TGG - chr4_4:56427078-56427097 Msa0689370:intron 30.0%
AAGTTTAAGCGTGGAAAATT+TGG - chr4_4:56427165-56427184 Msa0689370:CDS 30.0%
!!! AGCAAGTTTTGAATTTCAGT+TGG - chr4_4:56427294-56427313 Msa0689370:CDS 30.0%
ACAGACCTAAATAAAGTTGA+GGG + chr4_4:56427402-56427421 None:intergenic 30.0%
AACAGACCTAAATAAAGTTG+AGG + chr4_4:56427403-56427422 None:intergenic 30.0%
! CAACTTTATTTAGGTCTGTT+TGG - chr4_4:56427403-56427422 Msa0689370:CDS 30.0%
! GGTCTGTTTTCACATATAAC+AGG - chr4_4:56426221-56426240 Msa0689370:three_prime_UTR 35.0%
!! AGATGGTTGATGATGATATC+CGG + chr4_4:56426417-56426436 None:intergenic 35.0%
TATTGTGGAGCATGATAAGA+TGG + chr4_4:56426434-56426453 None:intergenic 35.0%
TCTTATCATGCTCCACAATA+TGG - chr4_4:56426434-56426453 Msa0689370:CDS 35.0%
CAAAGACGTAATATCAGGTA+TGG + chr4_4:56426883-56426902 None:intergenic 35.0%
! TATTGTTGTTATAAAGGGCG+TGG - chr4_4:56426979-56426998 Msa0689370:intron 35.0%
GTATAGGAGGATTAATTGCA+GGG - chr4_4:56427007-56427026 Msa0689370:intron 35.0%
TATAGGAGGATTAATTGCAG+GGG - chr4_4:56427008-56427027 Msa0689370:intron 35.0%
ATGGTTATGGTTATGGACAT+GGG - chr4_4:56427085-56427104 Msa0689370:intron 35.0%
! GTCTTTTGTTCAAATGTGTG+TGG - chr4_4:56427356-56427375 Msa0689370:CDS 35.0%
! GAAAGCCCTCAACTTTATTT+AGG - chr4_4:56427394-56427413 Msa0689370:CDS 35.0%
! GGTCTGTTTTCACATATAAC+AGG - chr4_4:56426221-56426240 Msa0689370:three_prime_UTR 35.0%
!! AGATGGTTGATGATGATATC+CGG + chr4_4:56426417-56426436 None:intergenic 35.0%
TATTGTGGAGCATGATAAGA+TGG + chr4_4:56426434-56426453 None:intergenic 35.0%
TCTTATCATGCTCCACAATA+TGG - chr4_4:56426434-56426453 Msa0689370:CDS 35.0%
CAAAGACGTAATATCAGGTA+TGG + chr4_4:56426883-56426902 None:intergenic 35.0%
! TATTGTTGTTATAAAGGGCG+TGG - chr4_4:56426979-56426998 Msa0689370:intron 35.0%
GTATAGGAGGATTAATTGCA+GGG - chr4_4:56427007-56427026 Msa0689370:intron 35.0%
TATAGGAGGATTAATTGCAG+GGG - chr4_4:56427008-56427027 Msa0689370:intron 35.0%
ATGGTTATGGTTATGGACAT+GGG - chr4_4:56427085-56427104 Msa0689370:intron 35.0%
! GTCTTTTGTTCAAATGTGTG+TGG - chr4_4:56427356-56427375 Msa0689370:CDS 35.0%
! GAAAGCCCTCAACTTTATTT+AGG - chr4_4:56427394-56427413 Msa0689370:CDS 35.0%
GATCAAGAATCTAGTGAGAG+AGG - chr4_4:56426200-56426219 Msa0689370:three_prime_UTR 40.0%
TCTTATTCCTATCCACCTCA+AGG - chr4_4:56426281-56426300 Msa0689370:three_prime_UTR 40.0%
GAAGGATAATAACCTCCTTG+AGG + chr4_4:56426338-56426357 None:intergenic 40.0%
ATATCCGGAATGAGGATAAC+CGG + chr4_4:56426402-56426421 None:intergenic 40.0%
! TGATGATGATATCCGGAATG+AGG + chr4_4:56426410-56426429 None:intergenic 40.0%
GGATATGGATAGCCATATTG+TGG + chr4_4:56426449-56426468 None:intergenic 40.0%
TATGGCTATCCATATCCTTC+TGG - chr4_4:56426452-56426471 Msa0689370:CDS 40.0%
TCAAGTGTACCAGAAGGATA+TGG + chr4_4:56426464-56426483 None:intergenic 40.0%
!! CGGCTTGTTAAAATTCTGCT+AGG + chr4_4:56426629-56426648 None:intergenic 40.0%
GGTATAGGAGGATTAATTGC+AGG - chr4_4:56427006-56427025 Msa0689370:intron 40.0%
GGAGGTTATCATCATGGTTA+TGG - chr4_4:56427072-56427091 Msa0689370:intron 40.0%
CATGGTTATGGTTATGGACA+TGG - chr4_4:56427084-56427103 Msa0689370:intron 40.0%
ATGGAAAATTTGGCAAGCGT+TGG - chr4_4:56427118-56427137 Msa0689370:intron 40.0%
!! AAGCATGGTAGGTTTGGTTT+TGG - chr4_4:56427141-56427160 Msa0689370:intron 40.0%
!!! GGTTTTGGAAAGTTTAAGCG+TGG - chr4_4:56427156-56427175 Msa0689370:intron 40.0%
GATCAAGAATCTAGTGAGAG+AGG - chr4_4:56426200-56426219 Msa0689370:three_prime_UTR 40.0%
TCTTATTCCTATCCACCTCA+AGG - chr4_4:56426281-56426300 Msa0689370:three_prime_UTR 40.0%
GAAGGATAATAACCTCCTTG+AGG + chr4_4:56426338-56426357 None:intergenic 40.0%
ATATCCGGAATGAGGATAAC+CGG + chr4_4:56426402-56426421 None:intergenic 40.0%
! TGATGATGATATCCGGAATG+AGG + chr4_4:56426410-56426429 None:intergenic 40.0%
GGATATGGATAGCCATATTG+TGG + chr4_4:56426449-56426468 None:intergenic 40.0%
TATGGCTATCCATATCCTTC+TGG - chr4_4:56426452-56426471 Msa0689370:CDS 40.0%
TCAAGTGTACCAGAAGGATA+TGG + chr4_4:56426464-56426483 None:intergenic 40.0%
!! CGGCTTGTTAAAATTCTGCT+AGG + chr4_4:56426629-56426648 None:intergenic 40.0%
GGTATAGGAGGATTAATTGC+AGG - chr4_4:56427006-56427025 Msa0689370:intron 40.0%
GGAGGTTATCATCATGGTTA+TGG - chr4_4:56427072-56427091 Msa0689370:intron 40.0%
CATGGTTATGGTTATGGACA+TGG - chr4_4:56427084-56427103 Msa0689370:intron 40.0%
ATGGAAAATTTGGCAAGCGT+TGG - chr4_4:56427118-56427137 Msa0689370:intron 40.0%
!! AAGCATGGTAGGTTTGGTTT+TGG - chr4_4:56427141-56427160 Msa0689370:intron 40.0%
!!! GGTTTTGGAAAGTTTAAGCG+TGG - chr4_4:56427156-56427175 Msa0689370:intron 40.0%
TAGGAATAAGAACCAGGTGG+AGG + chr4_4:56426272-56426291 None:intergenic 45.0%
GGATAGGAATAAGAACCAGG+TGG + chr4_4:56426275-56426294 None:intergenic 45.0%
GGTGGATAGGAATAAGAACC+AGG + chr4_4:56426278-56426297 None:intergenic 45.0%
TATTCCTATCCACCTCAAGG+AGG - chr4_4:56426284-56426303 Msa0689370:three_prime_UTR 45.0%
ATAACCTCCTTGAGGTGGAT+AGG + chr4_4:56426291-56426310 None:intergenic 45.0%
GGATAATAACCTCCTTGAGG+TGG + chr4_4:56426296-56426315 None:intergenic 45.0%
GGTGGATAATAACCTCCTTG+AGG + chr4_4:56426299-56426318 None:intergenic 45.0%
AGGTTATTATCCACCGCAAC+CGG - chr4_4:56426304-56426323 Msa0689370:three_prime_UTR 45.0%
GGATAATAACCTCCTTGAGG+TGG + chr4_4:56426296-56426315 None:intergenic 45.0%
!! GCATGTGGTGGATATTGTGA+AGG + chr4_4:56426356-56426375 None:intergenic 45.0%
GGATGATCAAGTGTACCAGA+AGG + chr4_4:56426470-56426489 None:intergenic 45.0%
CAGAATGATGCTCGTTTGAG+AGG - chr4_4:56426931-56426950 Msa0689370:intron 45.0%
! TGTTATAAAGGGCGTGGATC+TGG - chr4_4:56426985-56427004 Msa0689370:intron 45.0%
GGACATGGAGGTTATCATCA+TGG - chr4_4:56427066-56427085 Msa0689370:intron 45.0%
! GGACATGGGAAGTATAAGCA+TGG - chr4_4:56427099-56427118 Msa0689370:intron 45.0%
GTGGAAAATTTGGCAAGCGA+TGG - chr4_4:56427175-56427194 Msa0689370:CDS 45.0%
TAGGAATAAGAACCAGGTGG+AGG + chr4_4:56426272-56426291 None:intergenic 45.0%
GGATAGGAATAAGAACCAGG+TGG + chr4_4:56426275-56426294 None:intergenic 45.0%
GGTGGATAGGAATAAGAACC+AGG + chr4_4:56426278-56426297 None:intergenic 45.0%
TATTCCTATCCACCTCAAGG+AGG - chr4_4:56426284-56426303 Msa0689370:three_prime_UTR 45.0%
ATAACCTCCTTGAGGTGGAT+AGG + chr4_4:56426291-56426310 None:intergenic 45.0%
GGATAATAACCTCCTTGAGG+TGG + chr4_4:56426296-56426315 None:intergenic 45.0%
GGTGGATAATAACCTCCTTG+AGG + chr4_4:56426299-56426318 None:intergenic 45.0%
AGGTTATTATCCACCGCAAC+CGG - chr4_4:56426304-56426323 Msa0689370:three_prime_UTR 45.0%
GGATAATAACCTCCTTGAGG+TGG + chr4_4:56426296-56426315 None:intergenic 45.0%
!! GCATGTGGTGGATATTGTGA+AGG + chr4_4:56426356-56426375 None:intergenic 45.0%
GGATGATCAAGTGTACCAGA+AGG + chr4_4:56426470-56426489 None:intergenic 45.0%
CAGAATGATGCTCGTTTGAG+AGG - chr4_4:56426931-56426950 Msa0689370:intron 45.0%
! TGTTATAAAGGGCGTGGATC+TGG - chr4_4:56426985-56427004 Msa0689370:intron 45.0%
GGACATGGAGGTTATCATCA+TGG - chr4_4:56427066-56427085 Msa0689370:intron 45.0%
! GGACATGGGAAGTATAAGCA+TGG - chr4_4:56427099-56427118 Msa0689370:intron 45.0%
GTGGAAAATTTGGCAAGCGA+TGG - chr4_4:56427175-56427194 Msa0689370:CDS 45.0%
GAATAAGAACCAGGTGGAGG+AGG + chr4_4:56426269-56426288 None:intergenic 50.0%
CAATATCCACCACATGCTGC+AGG - chr4_4:56426359-56426378 Msa0689370:three_prime_UTR 50.0%
GAATGAGGATAACCGGAAGG+TGG + chr4_4:56426395-56426414 None:intergenic 50.0%
CCTTCCGGTTATCCTCATTC+CGG - chr4_4:56426395-56426414 Msa0689370:three_prime_UTR 50.0%
! AAAGGGCGTGGATCTGGTAT+AGG - chr4_4:56426991-56427010 Msa0689370:intron 50.0%
AGGAGGATTAATTGCAGGGG+CGG - chr4_4:56427011-56427030 Msa0689370:intron 50.0%
!! TTTGGCAAGCGTTGGAAGCA+TGG - chr4_4:56427126-56427145 Msa0689370:intron 50.0%
! CGTTGGAAGCATGGTAGGTT+TGG - chr4_4:56427135-56427154 Msa0689370:intron 50.0%
CAAGCGATGGAAGTGATGCA+GGG - chr4_4:56427188-56427207 Msa0689370:CDS 50.0%
AAGCGATGGAAGTGATGCAG+GGG - chr4_4:56427189-56427208 Msa0689370:CDS 50.0%
GAATAAGAACCAGGTGGAGG+AGG + chr4_4:56426269-56426288 None:intergenic 50.0%
CAATATCCACCACATGCTGC+AGG - chr4_4:56426359-56426378 Msa0689370:three_prime_UTR 50.0%
GAATGAGGATAACCGGAAGG+TGG + chr4_4:56426395-56426414 None:intergenic 50.0%
CCTTCCGGTTATCCTCATTC+CGG - chr4_4:56426395-56426414 Msa0689370:three_prime_UTR 50.0%
! AAAGGGCGTGGATCTGGTAT+AGG - chr4_4:56426991-56427010 Msa0689370:intron 50.0%
AGGAGGATTAATTGCAGGGG+CGG - chr4_4:56427011-56427030 Msa0689370:intron 50.0%
!! TTTGGCAAGCGTTGGAAGCA+TGG - chr4_4:56427126-56427145 Msa0689370:intron 50.0%
! CGTTGGAAGCATGGTAGGTT+TGG - chr4_4:56427135-56427154 Msa0689370:intron 50.0%
CAAGCGATGGAAGTGATGCA+GGG - chr4_4:56427188-56427207 Msa0689370:CDS 50.0%
AAGCGATGGAAGTGATGCAG+GGG - chr4_4:56427189-56427208 Msa0689370:CDS 50.0%
GAACCAGGTGGAGGAGGATA+AGG + chr4_4:56426263-56426282 None:intergenic 55.0%
! TGAGGTGGATATGCCGGTTG+CGG + chr4_4:56426320-56426339 None:intergenic 55.0%
CAACCGGCATATCCACCTCA+AGG - chr4_4:56426320-56426339 Msa0689370:three_prime_UTR 55.0%
CCTCCTTGAGGTGGATATGC+CGG + chr4_4:56426326-56426345 None:intergenic 55.0%
TATCCACCACATGCTGCAGG+AGG - chr4_4:56426362-56426381 Msa0689370:three_prime_UTR 55.0%
CCGGAATGAGGATAACCGGA+AGG + chr4_4:56426398-56426417 None:intergenic 55.0%
CACCATCTGTCGCATGGACA+TGG - chr4_4:56427051-56427070 Msa0689370:intron 55.0%
CTCCATGTCCATGCGACAGA+TGG + chr4_4:56427056-56427075 None:intergenic 55.0%
CATCTGTCGCATGGACATGG+AGG - chr4_4:56427054-56427073 Msa0689370:intron 55.0%
!! GCAAGCGTTGGAAGCATGGT+AGG - chr4_4:56427130-56427149 Msa0689370:intron 55.0%
GCAAGCGATGGAAGTGATGC+AGG - chr4_4:56427187-56427206 Msa0689370:CDS 55.0%
GAACCAGGTGGAGGAGGATA+AGG + chr4_4:56426263-56426282 None:intergenic 55.0%
! TGAGGTGGATATGCCGGTTG+CGG + chr4_4:56426320-56426339 None:intergenic 55.0%
CAACCGGCATATCCACCTCA+AGG - chr4_4:56426320-56426339 Msa0689370:three_prime_UTR 55.0%
CCTCCTTGAGGTGGATATGC+CGG + chr4_4:56426326-56426345 None:intergenic 55.0%
TATCCACCACATGCTGCAGG+AGG - chr4_4:56426362-56426381 Msa0689370:three_prime_UTR 55.0%
CCGGAATGAGGATAACCGGA+AGG + chr4_4:56426398-56426417 None:intergenic 55.0%
CACCATCTGTCGCATGGACA+TGG - chr4_4:56427051-56427070 Msa0689370:intron 55.0%
CTCCATGTCCATGCGACAGA+TGG + chr4_4:56427056-56427075 None:intergenic 55.0%
CATCTGTCGCATGGACATGG+AGG - chr4_4:56427054-56427073 Msa0689370:intron 55.0%
!! GCAAGCGTTGGAAGCATGGT+AGG - chr4_4:56427130-56427149 Msa0689370:intron 55.0%
GCAAGCGATGGAAGTGATGC+AGG - chr4_4:56427187-56427206 Msa0689370:CDS 55.0%
!! GGAGGATAAGGTGGTGCTGC+TGG + chr4_4:56426251-56426270 None:intergenic 60.0%
CCAGGTGGAGGAGGATAAGG+TGG + chr4_4:56426260-56426279 None:intergenic 60.0%
CCACCTTATCCTCCTCCACC+TGG - chr4_4:56426257-56426276 Msa0689370:three_prime_UTR 60.0%
CCGGCATATCCACCTCAAGG+AGG - chr4_4:56426323-56426342 Msa0689370:three_prime_UTR 60.0%
CACCACATGCTGCAGGAGGA+GGG - chr4_4:56426366-56426385 Msa0689370:three_prime_UTR 60.0%
TACCCTCCTCCTGCAGCATG+TGG + chr4_4:56426371-56426390 None:intergenic 60.0%
!! GGAGGAGGGTATCCACCTTC+CGG - chr4_4:56426380-56426399 Msa0689370:three_prime_UTR 60.0%
GGGCGTGGATCTGGTATAGG+AGG - chr4_4:56426994-56427013 Msa0689370:intron 60.0%
GGTGCTCACCATCTGTCGCA+TGG - chr4_4:56427045-56427064 Msa0689370:intron 60.0%
!! GGAGGATAAGGTGGTGCTGC+TGG + chr4_4:56426251-56426270 None:intergenic 60.0%
CCAGGTGGAGGAGGATAAGG+TGG + chr4_4:56426260-56426279 None:intergenic 60.0%
CCACCTTATCCTCCTCCACC+TGG - chr4_4:56426257-56426276 Msa0689370:three_prime_UTR 60.0%
CCGGCATATCCACCTCAAGG+AGG - chr4_4:56426323-56426342 Msa0689370:three_prime_UTR 60.0%
CACCACATGCTGCAGGAGGA+GGG - chr4_4:56426366-56426385 Msa0689370:three_prime_UTR 60.0%
TACCCTCCTCCTGCAGCATG+TGG + chr4_4:56426371-56426390 None:intergenic 60.0%
!! GGAGGAGGGTATCCACCTTC+CGG - chr4_4:56426380-56426399 Msa0689370:three_prime_UTR 60.0%
GGGCGTGGATCTGGTATAGG+AGG - chr4_4:56426994-56427013 Msa0689370:intron 60.0%
GGTGCTCACCATCTGTCGCA+TGG - chr4_4:56427045-56427064 Msa0689370:intron 60.0%
! GGTGGATATGCCGGTTGCGG+TGG + chr4_4:56426317-56426336 None:intergenic 65.0%
CCTCCTCCTGCAGCATGTGG+TGG + chr4_4:56426368-56426387 None:intergenic 65.0%
CCACCACATGCTGCAGGAGG+AGG - chr4_4:56426365-56426384 Msa0689370:three_prime_UTR 65.0%
! GGTGGATATGCCGGTTGCGG+TGG + chr4_4:56426317-56426336 None:intergenic 65.0%
CCTCCTCCTGCAGCATGTGG+TGG + chr4_4:56426368-56426387 None:intergenic 65.0%
CCACCACATGCTGCAGGAGG+AGG - chr4_4:56426365-56426384 Msa0689370:three_prime_UTR 65.0%
GCAGGGGCGGCAGCTGCATA+CGG - chr4_4:56427024-56427043 Msa0689370:intron 70.0%
GCAGGGGCGGCAGCTGCATA+CGG - chr4_4:56427024-56427043 Msa0689370:intron 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 56426176 56427458 56426176 ID=Msa0689370;Name=Msa0689370
chr4_4 mRNA 56426176 56427458 56426176 ID=Msa0689370-mRNA-1;Parent=Msa0689370;Name=Msa0689370-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|1|0.5|1|1|1|2|255|168
chr4_4 exon 56426176 56426639 56426176 ID=Msa0689370-mRNA-1:exon:14927;Parent=Msa0689370-mRNA-1
chr4_4 exon 56427161 56427458 56427161 ID=Msa0689370-mRNA-1:exon:14926;Parent=Msa0689370-mRNA-1
chr4_4 CDS 56427161 56427458 56427161 ID=Msa0689370-mRNA-1:cds;Parent=Msa0689370-mRNA-1
chr4_4 CDS 56426431 56426639 56426431 ID=Msa0689370-mRNA-1:cds;Parent=Msa0689370-mRNA-1
chr4_4 three_prime_UTR 56426176 56426430 56426176 ID=Msa0689370-mRNA-1:three_prime_utr;Parent=Msa0689370-mRNA-1
Gene Sequence

>Msa0689370

ATGAACAATGGTAGAAACAAGCATGATCAAGAATCTAGTGAGAGAGGTCTGTTTTCACATATAACAGGCTATCCAGCAGCACCACCTTATCCTCCTCCACCTGGTTCTTATTCCTATCCACCTCAAGGAGGTTATTATCCACCGCAACCGGCATATCCACCTCAAGGAGGTTATTATCCTTCACAATATCCACCACATGCTGCAGGAGGAGGGTATCCACCTTCCGGTTATCCTCATTCCGGATATCATCATCAACCATCTTATCATGCTCCACAATATGGCTATCCATATCCTTCTGGGCGTGGATCTGGTATAGGAGGATTAATTGCAGGGGCGGCAGCTGCATACGGTGCTCACCATCTGTCGCATGGACATGGAGGTTATCATCATGGTTATGGTTATGGACATGGGAAGTATAAGCATGGAAAATTTGGCAAGCGTTGGAAGCATGGTAGGTTTGGTTTTGGAAAGTTTAAGCGTGGAAAATTTGGCAAGCGATGGAAGTGA
Protein sequence

>Msa0689370

MNNGRNKHDQESSERGLFSHITGYPAAPPYPPPPGSYSYPPQGGYYPPQPAYPPQGGYYPSQYPPHAAGGGYPPSGYPHSGYHHQPSYHAPQYGYPYPSGRGSGIGGLIAGAAAAYGAHHLSHGHGGYHHGYGYGHGKYKHGKFGKRWKHGRFGFGKFKRGKFGKRWK*